灯笼草对致敏小鼠肝损伤的保护作用
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摘要
目的观察致敏小鼠是否确实存在肝损伤，探讨灯笼草果实及宿萼对卵清蛋白致敏小鼠肝损伤的影响，研究灯笼草防治小儿食物过敏更有效的药用部分。
方法制备小鼠过敏模型；灌服不同浓度的灯笼草果实和宿萼的水提物；采取血液和肝脏标本，间接测定血清中IgE及ALT水平；并对肝脏样本进行HE染色及免疫组织化学染色，检测TNF-α、IFN-γ的阳性率；并对果实和宿萼组的药效作用进行比较研究。
结果1．激发组小鼠IgE、ALT、TNF-α和IFN-γ含量均明显升高；2．高、中浓度灯笼草果实和宿萼，均可明显抑制卵清蛋白介导的小鼠被动过敏反应(P<0.01>，缓解小鼠过敏引起的肝脏炎症；实验组小鼠的IgE、ALT、TNF-α和IFN-γ含量明显低于OVA激发组，差异有显著性(P<0.01)，低浓度组不明显。3．宿萼组降低IgE、ALT、TNF-α和IFN-γ的作用明显优于果实组(P<0.05)。
结论1．致敏小鼠除肠道、皮肤、呼吸道炎变外，确实存在明显的肝损伤，这种损伤可能是由IgE介导的速发型免疫反应，并且与细胞因子IFN-γ和TNF-α有密切关系。2．高、中浓度灯笼草果实及宿萼对致敏小鼠的免疫性肝损伤均有明显的保护作用。3．灯笼草宿萼较果实对致敏小鼠抗炎抗过敏及保护肝损伤的作用更加明显。4．灯笼草对治疗小儿食物过敏所致肝损伤可能具有一定的疗效。
Objective: To observation sends the sensitive ratwhether truly has the liver damage, to investigate theinfluence of physalis Peruviana L on OVA induced senstitiveliver injury. To approach the medicinal purposes part ofchildren food allergy.
Methods: Preparation small rat allergy model. Fill thedifferent density the physalis Peruviana L's fruit and calyxshuitiwu. Adopting blood and liver and indirect determinationblood serum IgE and ALT level; liver pathological changesunder microscope were observed. Histochemistry dyeingexamines the TNF-α、IFN-γmasculine gender rate. And Calyxgroup's fuction conducts the comparison rearch to the fruit.
Results: 1.The stimulations groups rat IgE、ALT、TNF-α、IFN-γObviously elevates content; 2.The high and middledoseage of might inhibit the dermato-allergic reaction of ratand mitigating the inflammation of intestine. Blood serumIgE and ALT in the model group were higher than in normalcontrol group (p＜0.01) the quantity of TNF-α、IFN-γwere higher than in normal group(p＜0.01). Which proved that OVAintravenously had already led to rat liver damage. Calyxgroup'change is more oboiously.
Conclusions: 1) Outside sends the sensitive rat exceptthe intestinal tract, the skin, the respiratory tract inflammationchanges, truly has the obvious liver damage, this kind ofdamage is the fast hairstyle immune response which maybelies between by IgE leads, and the cell factor TNF-α、IFN-γhas the close relation. 2) The high and middledoseagesphysalis Peruviana L's fruit and Sue sends the sensitive rat'simmunity liver damage to the OVA to the obviously protectivefunction. 3) To compare with fruit and Calyx to the OVA withthe result that the anti-inflammation. Anti-allergy fuction ismore obviously. 4) The physalis Peruviana L has somepossibly curative effect of children food allergy.
方法制备小鼠过敏模型；灌服不同浓度的灯笼草果实和宿萼的水提物；采取血液和肝脏标本，间接测定血清中IgE及ALT水平；并对肝脏样本进行HE染色及免疫组织化学染色，检测TNF-α、IFN-γ的阳性率；并对果实和宿萼组的药效作用进行比较研究。
结果1．激发组小鼠IgE、ALT、TNF-α和IFN-γ含量均明显升高；2．高、中浓度灯笼草果实和宿萼，均可明显抑制卵清蛋白介导的小鼠被动过敏反应(P<0.01>，缓解小鼠过敏引起的肝脏炎症；实验组小鼠的IgE、ALT、TNF-α和IFN-γ含量明显低于OVA激发组，差异有显著性(P<0.01)，低浓度组不明显。3．宿萼组降低IgE、ALT、TNF-α和IFN-γ的作用明显优于果实组(P<0.05)。
结论1．致敏小鼠除肠道、皮肤、呼吸道炎变外，确实存在明显的肝损伤，这种损伤可能是由IgE介导的速发型免疫反应，并且与细胞因子IFN-γ和TNF-α有密切关系。2．高、中浓度灯笼草果实及宿萼对致敏小鼠的免疫性肝损伤均有明显的保护作用。3．灯笼草宿萼较果实对致敏小鼠抗炎抗过敏及保护肝损伤的作用更加明显。4．灯笼草对治疗小儿食物过敏所致肝损伤可能具有一定的疗效。
Objective: To observation sends the sensitive ratwhether truly has the liver damage, to investigate theinfluence of physalis Peruviana L on OVA induced senstitiveliver injury. To approach the medicinal purposes part ofchildren food allergy.
Methods: Preparation small rat allergy model. Fill thedifferent density the physalis Peruviana L's fruit and calyxshuitiwu. Adopting blood and liver and indirect determinationblood serum IgE and ALT level; liver pathological changesunder microscope were observed. Histochemistry dyeingexamines the TNF-α、IFN-γmasculine gender rate. And Calyxgroup's fuction conducts the comparison rearch to the fruit.
Results: 1.The stimulations groups rat IgE、ALT、TNF-α、IFN-γObviously elevates content; 2.The high and middledoseage of might inhibit the dermato-allergic reaction of ratand mitigating the inflammation of intestine. Blood serumIgE and ALT in the model group were higher than in normalcontrol group (p＜0.01) the quantity of TNF-α、IFN-γwere higher than in normal group(p＜0.01). Which proved that OVAintravenously had already led to rat liver damage. Calyxgroup'change is more oboiously.
Conclusions: 1) Outside sends the sensitive rat exceptthe intestinal tract, the skin, the respiratory tract inflammationchanges, truly has the obvious liver damage, this kind ofdamage is the fast hairstyle immune response which maybelies between by IgE leads, and the cell factor TNF-α、IFN-γhas the close relation. 2) The high and middledoseagesphysalis Peruviana L's fruit and Sue sends the sensitive rat'simmunity liver damage to the OVA to the obviously protectivefunction. 3) To compare with fruit and Calyx to the OVA withthe result that the anti-inflammation. Anti-allergy fuction ismore obviously. 4) The physalis Peruviana L has somepossibly curative effect of children food allergy.
引文
[1] Hughes DA, Mills C.[J].Biologist, 2001, 48 (5):201-204.
[2] Chandr RK.[J].Am J Clin Nutr, 1997, 66: 526s-529s.
[3] 胡燕，黎海芪．[J]．中华儿科杂志，2000，38(7)：431—434
[4] 付萍．食物过敏与营养健康[J]．国外医学卫生学分册，2004，31(2)： 111-115
[5] Y,Ueno K,Yofu S,et al.The expression of IL-4, IL-6 and TNF-α in food sensitized mice after oral challenge[J]. Int Arch Allergy Immunol, 1999, 118(2-4): 226-227
[6] Iikura Y ,Iwasaki A,Tsubaki T, et al. Study of liver function in infants with atopic dermatitis using 13C-Methacetin breath test[J]. Int Arch Allergy Immunol, 1995,107(1-3): 189-193
[7] 艾国平．肠道黏膜免疫的构成与功能[J]．免疫学杂志，2000，16(4)： 82-83
[8] 蓝程．食物过敏疾病的免疫发病机制研究进展[J]．国外医学．内科学分册，2001，28(10)：433-435
[9] Mosmann TR Cherwinski H ,Bond MW, et al. Two types of routine helper T cell clone.I.Definition according to profiles of hyphokine Activitiesand secreted proteins [J].J.Immunol, 1986, 136(5):2348-2357
[10] 畅阳，梁仁．白介素-10与支气管哮喘的发病[J]．国外医学内科学册 1998，20(5)：207-209．
[11] Mudler R, Chancz P,Campell A M, et al.Different cytokine patterns in bronchial biopsies in asthma and chronic bronchitis[J].J Respir Med, 1996,90(2):79-85
[12] Ganmer F, Leist M, Lohse AW, et al. Concanavalin A induced T-cell-mediated hepatic injury in mice: the role of tumor necrosis factor. Hepatology, 1995, 21(1): 190—198.
[13] Ksontini R, Colagiovanni DB, Joseqhs MD, et al. Disparate roles for TNF—alpha and Fas ligand in Concanavalin A induced hepatitis. J Im-nmnol, 1998, 160(8): 4082—4089.
[14] Kusters S, GantnerF, KunstleG, et al. Interferon gamma plays a critical role in T cell—dependent liver injury in mice initiated by Coneanavalin A. Gastroenterology, 1996, 111(2): 462—471.
[15] Knolle PA, Gerken G, Loser E, et al. R01e ofsinusoidal endothelial cells of the liver in Concanavalin Ainduced hepatic injury in mice. Hepatolo—gy, 1996, 24(4): 824—829.
[16] Tiegs G. Experimental hepatitis and role of cytokines. Acta Gastroen terol Belg, 1997, 60(2): 176—179.
[17] 骆抗先．乙型肝炎基础和临床．北京：人民卫生出版社，1997， 118-123．
[18] Minthara H, Uno M, Seki Y, et al. Critical involvement of interferon gamma in the pathogenesis of T-cell activation-associated hepatitis and regulatory mechanisms of interleukin-6 for the manifestations of hepatitis. Hepatology, 1996, 23(6): 1608—1615
[19] M. Kawai and T. Matsuura, S. Kyuno, H. Matsuki, M. Takenaka etc. A new physalin from physalis alkekengi structure of physalin L. [J]. Phytochemistry, 1987, 26: 3313
[20] LI L Y.PANG Z Y, wu Y L. Utilization and culture technology of grand berry[J]. China J Wilding Plant Resource(中国野生植物资源)，2001, 16(2): 94. 95.
[21] 国家药典委员会．中华人民共和国药典[M]．北京：化学工业出版社．296．1
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[23] 中国科学院植物研究所．中国高等植物图鉴(第三册)[M]．北京：科学出版社，1983．716
[1]．国家药典委员会．中华人民共和国药典[M]．北京：化学工业出版社，296．1
[2]．江苏新乡医学院．中药大词典(下)[M]．上海：上海科学技术出版社．1986．2532
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[4]．胡春霞，佟风琴．酸浆果皮色素的提取及稳定性研究[J]．辽宁师专学报，2001，3(2)：91—93
[5]．郭文场等．中国药用花卉[M]．北京：学术书刊出版社，1989．90-93
[6]．周小平，杨晓虹等酸浆果实与宿萼无机元素的比较分析[J]．白求恩医科大学学报，2001，27(4)：363-364
[7]．管宪环等．微量元素，1989，4：30
[8]．周静，王莉，李艳，鲁建疆．酸浆果实营养成分分析[J]．营养学报，1997，19(2)，243—245
[9]．杨晓虹，陈滴，周晓平．酸浆果实无机元素和氨基酸的含量测定[J]．人参研究，2000，12(2)：34—36
[10]．王和平，徐美术．锦灯笼降血糖作用的实验研究[1J]．中医药信息， 2004，21(1)：53—55
[11]．李方莲，范恩学．中药挂金灯的降血脂作用的实验研究[J]．中 国老年学杂志，2006，26(1)：91-92
[12]．龚珊，单立冬，张玉英等．挂金灯镇痛作用的实验观察[J]，苏州大学学报(医学版)2002，22(4)：380—382
[13]．甄清，李静等。锦灯笼宿萼提取物体外抗菌作用研究[J]，天然药物研究与开发 2006，18：273-274
[14]．李萍，盛巡．王晓中．酸浆苦素B对活化多性核中性细胞化学发光及H2O2产生的影响[J]．中国病理生理杂志，1996，12(6)：626
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[2] Chandr RK.[J].Am J Clin Nutr, 1997, 66: 526s-529s.
[3] 胡燕，黎海芪．[J]．中华儿科杂志，2000，38(7)：431—434
[4] 付萍．食物过敏与营养健康[J]．国外医学卫生学分册，2004，31(2)： 111-115
[5] Y,Ueno K,Yofu S,et al.The expression of IL-4, IL-6 and TNF-α in food sensitized mice after oral challenge[J]. Int Arch Allergy Immunol, 1999, 118(2-4): 226-227
[6] Iikura Y ,Iwasaki A,Tsubaki T, et al. Study of liver function in infants with atopic dermatitis using 13C-Methacetin breath test[J]. Int Arch Allergy Immunol, 1995,107(1-3): 189-193
[7] 艾国平．肠道黏膜免疫的构成与功能[J]．免疫学杂志，2000，16(4)： 82-83
[8] 蓝程．食物过敏疾病的免疫发病机制研究进展[J]．国外医学．内科学分册，2001，28(10)：433-435
[9] Mosmann TR Cherwinski H ,Bond MW, et al. Two types of routine helper T cell clone.I.Definition according to profiles of hyphokine Activitiesand secreted proteins [J].J.Immunol, 1986, 136(5):2348-2357
[10] 畅阳，梁仁．白介素-10与支气管哮喘的发病[J]．国外医学内科学册 1998，20(5)：207-209．
[11] Mudler R, Chancz P,Campell A M, et al.Different cytokine patterns in bronchial biopsies in asthma and chronic bronchitis[J].J Respir Med, 1996,90(2):79-85
[12] Ganmer F, Leist M, Lohse AW, et al. Concanavalin A induced T-cell-mediated hepatic injury in mice: the role of tumor necrosis factor. Hepatology, 1995, 21(1): 190—198.
[13] Ksontini R, Colagiovanni DB, Joseqhs MD, et al. Disparate roles for TNF—alpha and Fas ligand in Concanavalin A induced hepatitis. J Im-nmnol, 1998, 160(8): 4082—4089.
[14] Kusters S, GantnerF, KunstleG, et al. Interferon gamma plays a critical role in T cell—dependent liver injury in mice initiated by Coneanavalin A. Gastroenterology, 1996, 111(2): 462—471.
[15] Knolle PA, Gerken G, Loser E, et al. R01e ofsinusoidal endothelial cells of the liver in Concanavalin Ainduced hepatic injury in mice. Hepatolo—gy, 1996, 24(4): 824—829.
[16] Tiegs G. Experimental hepatitis and role of cytokines. Acta Gastroen terol Belg, 1997, 60(2): 176—179.
[17] 骆抗先．乙型肝炎基础和临床．北京：人民卫生出版社，1997， 118-123．
[18] Minthara H, Uno M, Seki Y, et al. Critical involvement of interferon gamma in the pathogenesis of T-cell activation-associated hepatitis and regulatory mechanisms of interleukin-6 for the manifestations of hepatitis. Hepatology, 1996, 23(6): 1608—1615
[19] M. Kawai and T. Matsuura, S. Kyuno, H. Matsuki, M. Takenaka etc. A new physalin from physalis alkekengi structure of physalin L. [J]. Phytochemistry, 1987, 26: 3313
[20] LI L Y.PANG Z Y, wu Y L. Utilization and culture technology of grand berry[J]. China J Wilding Plant Resource(中国野生植物资源)，2001, 16(2): 94. 95.
[21] 国家药典委员会．中华人民共和国药典[M]．北京：化学工业出版社．296．1
[22] 江苏新乡医学院．中药大词典(下)[M]．上海：上海科学技术出版社．1986．2532
[23] 中国科学院植物研究所．中国高等植物图鉴(第三册)[M]．北京：科学出版社，1983．716
[1]．国家药典委员会．中华人民共和国药典[M]．北京：化学工业出版社，296．1
[2]．江苏新乡医学院．中药大词典(下)[M]．上海：上海科学技术出版社．1986．2532
[3]．吉林省中医中药研究所．长白山植物药志[M]．长春：吉林人民出版社，1982．1005—1005
[4]．胡春霞，佟风琴．酸浆果皮色素的提取及稳定性研究[J]．辽宁师专学报，2001，3(2)：91—93
[5]．郭文场等．中国药用花卉[M]．北京：学术书刊出版社，1989．90-93
[6]．周小平，杨晓虹等酸浆果实与宿萼无机元素的比较分析[J]．白求恩医科大学学报，2001，27(4)：363-364
[7]．管宪环等．微量元素，1989，4：30
[8]．周静，王莉，李艳，鲁建疆．酸浆果实营养成分分析[J]．营养学报，1997，19(2)，243—245
[9]．杨晓虹，陈滴，周晓平．酸浆果实无机元素和氨基酸的含量测定[J]．人参研究，2000，12(2)：34—36
[10]．王和平，徐美术．锦灯笼降血糖作用的实验研究[1J]．中医药信息， 2004，21(1)：53—55
[11]．李方莲，范恩学．中药挂金灯的降血脂作用的实验研究[J]．中 国老年学杂志，2006，26(1)：91-92
[12]．龚珊，单立冬，张玉英等．挂金灯镇痛作用的实验观察[J]，苏州大学学报(医学版)2002，22(4)：380—382
[13]．甄清，李静等。锦灯笼宿萼提取物体外抗菌作用研究[J]，天然药物研究与开发 2006，18：273-274
[14]．李萍，盛巡．王晓中．酸浆苦素B对活化多性核中性细胞化学发光及H2O2产生的影响[J]．中国病理生理杂志，1996，12(6)：626
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