双峰驼自然发酵乳对小鼠抗应激和抗氧化能力的影响
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摘要
为探讨双峰驼自然发酵乳(酸驼乳)的抗应激和抗氧化作用,本实验以清洁级昆明种小白鼠为实验动物,给予不同剂量的双峰驼自然发酵乳一定时期后,较为系统地检测了受试小鼠的抗应激和抗氧化相关指标。在抗应激试验中,将实验动物随机分成3组,Ⅰ组为空白对照组,按0.3 mL /10g剂量灌服生理盐水;Ⅱ组为发酵驼乳低剂量组,Ⅲ组为发酵驼乳高剂量组,分别按0.15 mL /10g剂量和0.3 mL /10g剂量灌服双峰驼自然发酵乳,连续灌服7天,观察双峰驼自然发酵乳对小鼠负重游泳时间﹑耐常压缺氧时间﹑耐高温时间和耐低温时间的影响;定量检测对慢性热应激小鼠的日增重、饲料转化率、胸腺指数和脾脏指数以及血清葡萄糖、白蛋白和总蛋白含量、碱性磷酸酶(AKP)活力、肌酸激酶(CK)活力、乳酸脱氢酶(LDH)活力等的影响。抗氧化试验的分组、灌服发酵驼乳的途径和剂量同上。在末次灌服30min后,采血并处死取样,测定血清、肝、脑和骨骼肌的总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力,丙二醛(MDA)含量和总抗氧化能力(T-AOC)等。
试验结果显示,双峰驼自然发酵乳高剂量组能显著延长小鼠负重游泳时间﹑耐缺氧存活时间﹑耐高温存活时间、耐低温存活时间,极显著提高血清葡萄糖、总蛋白含量和极显著降低AKP、CK活性;白蛋白含量和LDH活性有所上升,但尚未达到显著水平;双峰驼自然发酵乳高剂量组还能显著或极显著(p<0.05或p<0.01)降低小鼠血清、肝、脑组织和骨骼肌MDA含量;能显著或极显著(p<0.05或p<0.01)提高血清、脑组织和骨骼肌T-SOD、CAT、GSH-Px和T-AOC活力,而对肝组织T-SOD、CAT、GSH-PX和T-AOC活力的影响则无明显的规律性。因此,双峰驼自然发酵乳具有一定的抗急慢性应激反应作用和较强的抗氧化作用。
To investigate the effects of bactrian camel fermented milk on the anti-stress and antioxidant capacity of mice, the some related indicators of anti-stress and antioxidant were systematically evaluated on kunming mice administered with different doses of camel milk in this study. In anti-stress experiment: the experimental animals were randomly allocated into 3 group of groupⅠ,ⅡandⅢ. GroupⅠwas the control, received 0.3 mL / 10g dose of normal saline; GroupⅡa ndⅢwere experimental group administered by per os with 0.15 mL/10g and 0.3 mL/10g of bactrian camel fermented milk respectively for 7 successive days. Then the effects of fermented camel milk on the time for loading swimming, hypoxia survival, heat resisting survival and cold resisting survival of mice were detected. As well as, the effects of fermented camel milk on the daily weight gain, feed conversion rate, thymus and spleen index, the content of serum glucose, albumin and total protein, the activity of alkaline phosphates (AKP), creatine kinase (CK) and lactate dehydrogenises (LDH) were examined in chronic heat stressed mice, respectively. In Antioxidation experiment: the dose and administration route of fermented camel milk were same as anti-stress experiment. After 30min of last administration, blood samples were collected and the activity of total superoxide dismutase(T-SOD), catalase (CAT), glutathione peroxidase (GST-PX), total antioxidation capacity (T-AOC) and the content of malondialdehyde (MDA) in liver, muscle and brain tissue were detected respectively.
The result showed that Bactrian camel fermented milk in high-dose group significantly extended the time of loading swimming, hypoxia survival, heat resisting survival of mice, and significantly increased the content of serum total protein and glucose and significantly decreased the activity of AKP and CK. Compared with the control group, the high dose of the Bactrian camel fermented milk significantly decreased the activities of GSH-PX, CAT and T-AOC and the content of MDA in serum, liver, muscle and brain tissue, respectively; significantly increased the activities of T-SOD, GSH-PX, CAT and T-AOC in serum, muscle and brain tissue, respectively. However, there were no significant regularity for the activities of T-SOD, CAT and T-AOC in liver at the high dose of the Bactrian camel fermented milk group. Therefore, Bactrian camel fermented milk has a certain effects on anti-stress and antioxidant capacity of mice.
试验结果显示,双峰驼自然发酵乳高剂量组能显著延长小鼠负重游泳时间﹑耐缺氧存活时间﹑耐高温存活时间、耐低温存活时间,极显著提高血清葡萄糖、总蛋白含量和极显著降低AKP、CK活性;白蛋白含量和LDH活性有所上升,但尚未达到显著水平;双峰驼自然发酵乳高剂量组还能显著或极显著(p<0.05或p<0.01)降低小鼠血清、肝、脑组织和骨骼肌MDA含量;能显著或极显著(p<0.05或p<0.01)提高血清、脑组织和骨骼肌T-SOD、CAT、GSH-Px和T-AOC活力,而对肝组织T-SOD、CAT、GSH-PX和T-AOC活力的影响则无明显的规律性。因此,双峰驼自然发酵乳具有一定的抗急慢性应激反应作用和较强的抗氧化作用。
To investigate the effects of bactrian camel fermented milk on the anti-stress and antioxidant capacity of mice, the some related indicators of anti-stress and antioxidant were systematically evaluated on kunming mice administered with different doses of camel milk in this study. In anti-stress experiment: the experimental animals were randomly allocated into 3 group of groupⅠ,ⅡandⅢ. GroupⅠwas the control, received 0.3 mL / 10g dose of normal saline; GroupⅡa ndⅢwere experimental group administered by per os with 0.15 mL/10g and 0.3 mL/10g of bactrian camel fermented milk respectively for 7 successive days. Then the effects of fermented camel milk on the time for loading swimming, hypoxia survival, heat resisting survival and cold resisting survival of mice were detected. As well as, the effects of fermented camel milk on the daily weight gain, feed conversion rate, thymus and spleen index, the content of serum glucose, albumin and total protein, the activity of alkaline phosphates (AKP), creatine kinase (CK) and lactate dehydrogenises (LDH) were examined in chronic heat stressed mice, respectively. In Antioxidation experiment: the dose and administration route of fermented camel milk were same as anti-stress experiment. After 30min of last administration, blood samples were collected and the activity of total superoxide dismutase(T-SOD), catalase (CAT), glutathione peroxidase (GST-PX), total antioxidation capacity (T-AOC) and the content of malondialdehyde (MDA) in liver, muscle and brain tissue were detected respectively.
The result showed that Bactrian camel fermented milk in high-dose group significantly extended the time of loading swimming, hypoxia survival, heat resisting survival of mice, and significantly increased the content of serum total protein and glucose and significantly decreased the activity of AKP and CK. Compared with the control group, the high dose of the Bactrian camel fermented milk significantly decreased the activities of GSH-PX, CAT and T-AOC and the content of MDA in serum, liver, muscle and brain tissue, respectively; significantly increased the activities of T-SOD, GSH-PX, CAT and T-AOC in serum, muscle and brain tissue, respectively. However, there were no significant regularity for the activities of T-SOD, CAT and T-AOC in liver at the high dose of the Bactrian camel fermented milk group. Therefore, Bactrian camel fermented milk has a certain effects on anti-stress and antioxidant capacity of mice.
引文
1宁夏农学院,内蒙古农牧学院主编.养驼学[M].北京:农业出版社,1983.2
2贺新民.中国骆驼资源图志[M].长沙:湖南科学技术出版社,2003:72-73.
3赵电波,吉日木图,刘红霞.内蒙古阿拉善盟双峰驼驼乳理化性质研究[J].乳业科学与技术,2005,(3):112-114.
4斯钦,刘颖,巴图.驼奶中氨基酸和人体必需微量金属元素的测定[J].宁夏大学学报(自然科学版),1999,3(1):68-69.
5陆东林,张静,何晓瑞.驼乳的化学成分和加工利用[J].中国乳业,2008(7):36-38.
6 Alhadrami GA, CamelA, RoginskiH, FuquayJW, FoxPF. Encyclopedia of Dairy Sciences[M]. UK:Academic Press2003.616～622.
7 MerinU,BernsteinSD,Bloch-DamtiN,etal.Acom-parative study of milk proteins in camel(Camelus dromedarius)andbovine colostrum[J].Livestock Product Sci,2001,67:297-301.
8 ElagamyEI.Effect of heat treatment on camel milk pro-teins with respect to antimicrobial factors:a comparison with cows’and buffalo milk protein[J].Food Chemistry,2000,68:227-232.
9 Alhadrami GA.Camel[A].Roginski H,Fuquay JW,Fox PF.Encyclopedia of Dairy Sciences[M].UK:Academic Press.2003,616-622.
10 Farah Z.Composition and characteristics of camel milk〔J〕.Journal of Dairy Research.1993,60:603-626.
11 Yagil R.Camel milk.A Review[J].International Journal of Animal Science.1987,(2):81-99.
12 YagilR,ZagorskiO,van Crefeld C,et al.Scienceand camel's milk production. Chameuxet dromedaries,an meauxl aitiers[M].Ed.Saint Marin,G.Expan tifique Francais,Paris,1994:75-79.
13范洪波,哈斯苏荣,吉日木图.驼乳及其抗微生物因子的特性[J].畜牧与饲料科学,2006(1):37-40.
14赵电波,白艳,张文彬.自然发酵双峰驼驼乳营养价值的研究[J].乳业科学与技术,2007(6):305-307.
15马敏.牛奶与酸奶哪个好[J].科学与生活,2005(5):44-45.
16拉·巴勒道尔吉,策·纳木斯来.蒙古艾日嘎[M].呼和浩持:内蒙古人民出版社,1990;137-138.
17乌·扎木苏.酸马奶疗法.呼和浩持:内蒙古人民出版社,1986.
18古丽孜亚·卡克巴依,新华·那比.新疆哈萨克族传统发酵驼乳抗炎作用的初步研究[J].中国乳品工业,2007,35(9):8-10.
19顾瑞霞,伊萌,等.乳酸菌抗肿瘤特性的研究进展[J].中国微生态学杂志.1999,8,11(4):253-255.
20郭伟剑,于尔辛,郑颂国,等.健脾理气药诱导人肝癌细胞SMMC27721凋亡的研究[J].世界华人消化杂志.2000,8(1),52-55.
21郭建功,王初一,哈斯苏荣,吉日木图,等.双峰驼自然发酵乳对小鼠腹水型肝癌细胞H22的影响[J].中国奶牛,2009(1):9-11.
22王初一,郭建功,哈斯苏荣,吉日木图,等.苏尼特双峰驼鲜驼乳对荷瘤小鼠免疫功能的影响及其抗肿瘤作用[J].食品科学,2009,30(3):270-273.
23 Zimecki M ,Artym J. The effect of psychic stress on the immune response[J]. Postepy Hig Med Dosw ,2004 ,8:166-175.
24 Shi Y,Devadas S,Greeneltch KM ,et al.Stressed to death: impli-cation of lymphocyte apoptosis for psychoneuroimmunology[J].Brain Behav Immun ,2003 ,17 (l) :18-26.
25柴磊,李联杰.猪应激及防止对策.畜牧兽医科技信息(J).2005,3:56-58
26 Rosch PJ. Stress and memory loss:some speculations and solutions[J]. Stress Med,1997 ,13 (1) :1 - 6.
27石慧琳,李树田,王冬兰.应激对大鼠摄食量的影响及其机制的探讨[J].中国公共卫生,2003 ,19 (10) :1220-1221
28盛清凯,武英,王文治.添加Vc对热应激蛋鸡免疫性能的影响[J].畜牧与兽医, 2006 , 38(1): 38-40.
29李俊杰,桑润滋,田树军.热应激与牛血液成分的变化[J ],家畜生态,2001 ,22(4): 56-59
30高春燕,田程瑞.枸杞多糖体外氧化特性研究[J].粮食与汕脂,2005,7:28-29.
31王振纲.谈谈自由基[N].健康报,2002,(2002-07-18).
32范娜,刘海樱,王威.五种水果提取物抗氧活性的研究[J].食品研究与开发,2006,27(3):1-3.
33傅伟.自由基生物学理论在运动医学中的应用与疲劳发生机制的新解释[J].体育科研,1992(1):39-41.
34 Davies J,Quintanilha AT,Brooks tissue damage produced by exercise[J].Biochem Biophy Res Comm, 1982,107(4):1198-1205.
35陈永昌,汪凤维,黄耀,等.应激时大鼠胃粘膜中MDA含量及SOD和GSH-Px活性的变化[J].镇江医学院学报,1996,6(3):213-214.
36陆任云,许燕.应激大鼠肾脏中MDA含量及GSH-Px、CAT和Ca - ATPase活性的变化[J].镇江医学院学报,1998,8(3): 291–292.
37 McCord J.Oxygen-derived free radicals in postischemic tissue in-jury New EnglJ Med,1985,312: 159.
38方允中,郑荣梁.自由基生物学的理论与应用[M].北京:科学出版社,2002:213-233.
39刘凤华,于同泉,吴国娟,等.热应激中蛋鸡血液和肝脏SOD活性的动态变化.北京农学院学报,2003,18(2):112-114.
40 Lay Jr DC,Wilson ME.Physiological Indicators of Stress in Domestic Livestock. Physiological Indicators of Stress(online lecture),2001.
41黄忠远,杨军平,邱丽瑛.逍遥丸对慢性心理应激小鼠免疫系统的影响[J].江西中医学院学报,2007,19(6):68-69.
42 Derry DM.Thyroid hormone therapy in patients infected with human immunodeficiency virus:a clinical approach to treatment.Med Hypotheses,1996,47:227-233.
43 Gciger PG,Lin F,Girotti AW.Selenoperoxidase mediated cytoprotection against the damaging effects of tertbutyl hydoperoxide on leukaemia lcukernia cells [J].Free RADIC Biol Med,1993,14(3):251-266.
44 Mutlu-Türkoglu U,Erbil Y,Oztezcan S,et al.The effect of selenium and/ or Vitamin E treatments on radiation-induced intestinal injury in rats[J].Life Sciences,2000,66(20):1905-1913.
45何激进,方热军.微量元素硒的研究进展[J].饲料博览,2007(11):52-54.
46王金秋,林德贵,王雷,宋筱瑜,等.不同硒化合物对小鼠抗氧化性损伤作用的研究[J].畜牧兽医学报,2007, 38(12):1373-1376.
47王胜林,林映才,郑梨.抗热应激剂对肥育猪血清生化指标的影响[J].中国畜牧杂志,2003,39(1):11-12.
48孙长勉,栾新红,曹中赞.维生素E对热应激条件下肉鸡甲状腺激素分泌的影响[J].沈阳农业大学学报,2002,33(5):360-362.
49何德肆,胡述光,袁慧.添加不同抗热应激剂对热应激奶牛体内微量元素影响的研究[J].家畜生态学报,2005,26(6):22-25.
50顾宪红.鸡对热应激反应及预防措施[J].中国饲料,1994,a(3): 15-16; 1994,b(4):34-35.
51刘风华,谢仲权.抗热应激中草药添加剂的研究[J].中兽医医药杂志,1999,24(1):17-18.
52吴德峰,胡美华,黄建晖.抗热应激中草药添加剂对奶牛血液生化指标和小白鼠热应激模型ACTH指标的影响[J].福建农林大学学报:自然科学版,2005,34(2),255-259.
53文凤云,董淑丽,李晓丽,等.热应激对獭兔血液生化指标的影响[J].饲养与繁殖,2009(3):17-20.
54夏万良,戴小莲,徐铁山,周汉林,孙卫平,黄显洲,等.海南黑山羊血液生化指标与体重的相关分析[J].热带农业科学,2009,29(9):9-13.
55艾春香,陈立侨,高露姣,温小波,江洪波,等.VC对河蟹血清和组织中超氧化物歧化酶及磷酸酶活性的影响[J].台湾海峡,2002,21(4):431-437.
56 Lew H,Pyke S,Quintanilha A.Changes in the glutathione status of plasma,liver andmuscle following exhaustive exercise in rats[J].FEBS Lett.1985,185:262-266.
57 Leeuwenburgh CJ,Hollander R,Ji LL,et al.Adaptations of glutathione antioxidant system to endurance t raining are tissue and muscle fiber specific[J].Am J Physiol.1997,272 :R363-R369.
58岳丽红,程广东,王长平,周清波等.西藏灵菇酸奶对衰老模型小鼠抗氧化作用的研究[J].佳木斯大学学报(自然科学版),2008,26(3):430-432.
59游秋云,陈靓.红芪提取物对小鼠抗应激作用的实验研究[J].湖北中医杂志,2006,2(28):15-16.
60杜念兴.兽医免疫学[M]北京:中国农业出版社,2000.
61蒋定文,郭明敢,李楚芳,等.高温对小鼠胸腺和脾脏T细胞亚群的影响[J].中国公共卫生,2001,17(10):890-892.
62曾静,罗海吉,张勇,等.L-精氨酸对热应激小鼠胸腺T细胞亚群的影响[J].热带医学杂志,2003,3(2):155-157.
63蒲万霞,董鹏程,戴凤菊,等.热应激对小鼠免疫功能的影响[J].中兽医医药杂志,2005,14(5):10.
64焦玉兰,翟超,史万玉,秦建华,包永占,钟秀会,赵玉龙,等.“抗暑一号”对热应激小鼠部分血液生理生化指标的影响[J].动物医学进展,2009,30(7):29-32.
65范石军.热应激对产蛋鸡体组织的过氧化损伤及抗氧化的营养素的协同保护效应[D].黑龙江哈尔滨:东北农业大学,1998.
66董淑丽,邓雯,雷雪芹.热应激对动物理化特性及生产性能的影响[J].河南科技大学学报(农学版),2003,23(1):59–62.
67樊华,樊丽,王峰,张淑.热应激对泌乳奶牛血液生化指标的影响[J].中国畜牧兽医,2007,34(4):45-46.
68郑卓夫.减轻家禽热应激的添加剂研究进展[J].中国畜牧杂志,1992,28(4):56-58.
69郑过灿,肖尚友,穆小静,等.自由基检测技术发展.广州化学,2006 ,31 (3):37-44.
70 Wojtaszewski JFP,Richter EA.Glucose utilization during exercise: inuence of endurance training[J].Acta Physiol Scand,1998,162:351-358.
71李秀娟,刘福英,张焕铃,等.复肝春六号对H22荷瘤小鼠的抑瘤及免疫的调节作用[J ].中国老年学杂志,2006(6):92-93.
72杨晓萍,罗祖友,吴谋成.油菜花粉多糖的制备及其对荷瘤小鼠的影响[J].食品科学,2005 ,26(12):202-204.
73张江巍,曹郁生,李海星等.乳酸菌抗氧化活性及检测方法[J].中国乳品工业,2005,33(9):53-56.
74 TERBLANCHE SE. the effects of exhaustive exercise on the activity leveles of catalasein various tissues of male and female rates[J]. cell biology international, 2000,23(11):749-753
75 V. A. Ilyukha.Superoxide Dismutase and Catalase in the Organs of Mammals of Different Ecogenesis[J] .Journal of Evolutionary Biochemistry and Physiology, 2001,37(3):241-245
76金鸣,李金荣,蔡亚欣等.红花水溶性成分抗氧化作用的研究[J].心肺血管病杂志,1998,17(4):227-279
2贺新民.中国骆驼资源图志[M].长沙:湖南科学技术出版社,2003:72-73.
3赵电波,吉日木图,刘红霞.内蒙古阿拉善盟双峰驼驼乳理化性质研究[J].乳业科学与技术,2005,(3):112-114.
4斯钦,刘颖,巴图.驼奶中氨基酸和人体必需微量金属元素的测定[J].宁夏大学学报(自然科学版),1999,3(1):68-69.
5陆东林,张静,何晓瑞.驼乳的化学成分和加工利用[J].中国乳业,2008(7):36-38.
6 Alhadrami GA, CamelA, RoginskiH, FuquayJW, FoxPF. Encyclopedia of Dairy Sciences[M]. UK:Academic Press2003.616～622.
7 MerinU,BernsteinSD,Bloch-DamtiN,etal.Acom-parative study of milk proteins in camel(Camelus dromedarius)andbovine colostrum[J].Livestock Product Sci,2001,67:297-301.
8 ElagamyEI.Effect of heat treatment on camel milk pro-teins with respect to antimicrobial factors:a comparison with cows’and buffalo milk protein[J].Food Chemistry,2000,68:227-232.
9 Alhadrami GA.Camel[A].Roginski H,Fuquay JW,Fox PF.Encyclopedia of Dairy Sciences[M].UK:Academic Press.2003,616-622.
10 Farah Z.Composition and characteristics of camel milk〔J〕.Journal of Dairy Research.1993,60:603-626.
11 Yagil R.Camel milk.A Review[J].International Journal of Animal Science.1987,(2):81-99.
12 YagilR,ZagorskiO,van Crefeld C,et al.Scienceand camel's milk production. Chameuxet dromedaries,an meauxl aitiers[M].Ed.Saint Marin,G.Expan tifique Francais,Paris,1994:75-79.
13范洪波,哈斯苏荣,吉日木图.驼乳及其抗微生物因子的特性[J].畜牧与饲料科学,2006(1):37-40.
14赵电波,白艳,张文彬.自然发酵双峰驼驼乳营养价值的研究[J].乳业科学与技术,2007(6):305-307.
15马敏.牛奶与酸奶哪个好[J].科学与生活,2005(5):44-45.
16拉·巴勒道尔吉,策·纳木斯来.蒙古艾日嘎[M].呼和浩持:内蒙古人民出版社,1990;137-138.
17乌·扎木苏.酸马奶疗法.呼和浩持:内蒙古人民出版社,1986.
18古丽孜亚·卡克巴依,新华·那比.新疆哈萨克族传统发酵驼乳抗炎作用的初步研究[J].中国乳品工业,2007,35(9):8-10.
19顾瑞霞,伊萌,等.乳酸菌抗肿瘤特性的研究进展[J].中国微生态学杂志.1999,8,11(4):253-255.
20郭伟剑,于尔辛,郑颂国,等.健脾理气药诱导人肝癌细胞SMMC27721凋亡的研究[J].世界华人消化杂志.2000,8(1),52-55.
21郭建功,王初一,哈斯苏荣,吉日木图,等.双峰驼自然发酵乳对小鼠腹水型肝癌细胞H22的影响[J].中国奶牛,2009(1):9-11.
22王初一,郭建功,哈斯苏荣,吉日木图,等.苏尼特双峰驼鲜驼乳对荷瘤小鼠免疫功能的影响及其抗肿瘤作用[J].食品科学,2009,30(3):270-273.
23 Zimecki M ,Artym J. The effect of psychic stress on the immune response[J]. Postepy Hig Med Dosw ,2004 ,8:166-175.
24 Shi Y,Devadas S,Greeneltch KM ,et al.Stressed to death: impli-cation of lymphocyte apoptosis for psychoneuroimmunology[J].Brain Behav Immun ,2003 ,17 (l) :18-26.
25柴磊,李联杰.猪应激及防止对策.畜牧兽医科技信息(J).2005,3:56-58
26 Rosch PJ. Stress and memory loss:some speculations and solutions[J]. Stress Med,1997 ,13 (1) :1 - 6.
27石慧琳,李树田,王冬兰.应激对大鼠摄食量的影响及其机制的探讨[J].中国公共卫生,2003 ,19 (10) :1220-1221
28盛清凯,武英,王文治.添加Vc对热应激蛋鸡免疫性能的影响[J].畜牧与兽医, 2006 , 38(1): 38-40.
29李俊杰,桑润滋,田树军.热应激与牛血液成分的变化[J ],家畜生态,2001 ,22(4): 56-59
30高春燕,田程瑞.枸杞多糖体外氧化特性研究[J].粮食与汕脂,2005,7:28-29.
31王振纲.谈谈自由基[N].健康报,2002,(2002-07-18).
32范娜,刘海樱,王威.五种水果提取物抗氧活性的研究[J].食品研究与开发,2006,27(3):1-3.
33傅伟.自由基生物学理论在运动医学中的应用与疲劳发生机制的新解释[J].体育科研,1992(1):39-41.
34 Davies J,Quintanilha AT,Brooks tissue damage produced by exercise[J].Biochem Biophy Res Comm, 1982,107(4):1198-1205.
35陈永昌,汪凤维,黄耀,等.应激时大鼠胃粘膜中MDA含量及SOD和GSH-Px活性的变化[J].镇江医学院学报,1996,6(3):213-214.
36陆任云,许燕.应激大鼠肾脏中MDA含量及GSH-Px、CAT和Ca - ATPase活性的变化[J].镇江医学院学报,1998,8(3): 291–292.
37 McCord J.Oxygen-derived free radicals in postischemic tissue in-jury New EnglJ Med,1985,312: 159.
38方允中,郑荣梁.自由基生物学的理论与应用[M].北京:科学出版社,2002:213-233.
39刘凤华,于同泉,吴国娟,等.热应激中蛋鸡血液和肝脏SOD活性的动态变化.北京农学院学报,2003,18(2):112-114.
40 Lay Jr DC,Wilson ME.Physiological Indicators of Stress in Domestic Livestock. Physiological Indicators of Stress(online lecture),2001.
41黄忠远,杨军平,邱丽瑛.逍遥丸对慢性心理应激小鼠免疫系统的影响[J].江西中医学院学报,2007,19(6):68-69.
42 Derry DM.Thyroid hormone therapy in patients infected with human immunodeficiency virus:a clinical approach to treatment.Med Hypotheses,1996,47:227-233.
43 Gciger PG,Lin F,Girotti AW.Selenoperoxidase mediated cytoprotection against the damaging effects of tertbutyl hydoperoxide on leukaemia lcukernia cells [J].Free RADIC Biol Med,1993,14(3):251-266.
44 Mutlu-Türkoglu U,Erbil Y,Oztezcan S,et al.The effect of selenium and/ or Vitamin E treatments on radiation-induced intestinal injury in rats[J].Life Sciences,2000,66(20):1905-1913.
45何激进,方热军.微量元素硒的研究进展[J].饲料博览,2007(11):52-54.
46王金秋,林德贵,王雷,宋筱瑜,等.不同硒化合物对小鼠抗氧化性损伤作用的研究[J].畜牧兽医学报,2007, 38(12):1373-1376.
47王胜林,林映才,郑梨.抗热应激剂对肥育猪血清生化指标的影响[J].中国畜牧杂志,2003,39(1):11-12.
48孙长勉,栾新红,曹中赞.维生素E对热应激条件下肉鸡甲状腺激素分泌的影响[J].沈阳农业大学学报,2002,33(5):360-362.
49何德肆,胡述光,袁慧.添加不同抗热应激剂对热应激奶牛体内微量元素影响的研究[J].家畜生态学报,2005,26(6):22-25.
50顾宪红.鸡对热应激反应及预防措施[J].中国饲料,1994,a(3): 15-16; 1994,b(4):34-35.
51刘风华,谢仲权.抗热应激中草药添加剂的研究[J].中兽医医药杂志,1999,24(1):17-18.
52吴德峰,胡美华,黄建晖.抗热应激中草药添加剂对奶牛血液生化指标和小白鼠热应激模型ACTH指标的影响[J].福建农林大学学报:自然科学版,2005,34(2),255-259.
53文凤云,董淑丽,李晓丽,等.热应激对獭兔血液生化指标的影响[J].饲养与繁殖,2009(3):17-20.
54夏万良,戴小莲,徐铁山,周汉林,孙卫平,黄显洲,等.海南黑山羊血液生化指标与体重的相关分析[J].热带农业科学,2009,29(9):9-13.
55艾春香,陈立侨,高露姣,温小波,江洪波,等.VC对河蟹血清和组织中超氧化物歧化酶及磷酸酶活性的影响[J].台湾海峡,2002,21(4):431-437.
56 Lew H,Pyke S,Quintanilha A.Changes in the glutathione status of plasma,liver andmuscle following exhaustive exercise in rats[J].FEBS Lett.1985,185:262-266.
57 Leeuwenburgh CJ,Hollander R,Ji LL,et al.Adaptations of glutathione antioxidant system to endurance t raining are tissue and muscle fiber specific[J].Am J Physiol.1997,272 :R363-R369.
58岳丽红,程广东,王长平,周清波等.西藏灵菇酸奶对衰老模型小鼠抗氧化作用的研究[J].佳木斯大学学报(自然科学版),2008,26(3):430-432.
59游秋云,陈靓.红芪提取物对小鼠抗应激作用的实验研究[J].湖北中医杂志,2006,2(28):15-16.
60杜念兴.兽医免疫学[M]北京:中国农业出版社,2000.
61蒋定文,郭明敢,李楚芳,等.高温对小鼠胸腺和脾脏T细胞亚群的影响[J].中国公共卫生,2001,17(10):890-892.
62曾静,罗海吉,张勇,等.L-精氨酸对热应激小鼠胸腺T细胞亚群的影响[J].热带医学杂志,2003,3(2):155-157.
63蒲万霞,董鹏程,戴凤菊,等.热应激对小鼠免疫功能的影响[J].中兽医医药杂志,2005,14(5):10.
64焦玉兰,翟超,史万玉,秦建华,包永占,钟秀会,赵玉龙,等.“抗暑一号”对热应激小鼠部分血液生理生化指标的影响[J].动物医学进展,2009,30(7):29-32.
65范石军.热应激对产蛋鸡体组织的过氧化损伤及抗氧化的营养素的协同保护效应[D].黑龙江哈尔滨:东北农业大学,1998.
66董淑丽,邓雯,雷雪芹.热应激对动物理化特性及生产性能的影响[J].河南科技大学学报(农学版),2003,23(1):59–62.
67樊华,樊丽,王峰,张淑.热应激对泌乳奶牛血液生化指标的影响[J].中国畜牧兽医,2007,34(4):45-46.
68郑卓夫.减轻家禽热应激的添加剂研究进展[J].中国畜牧杂志,1992,28(4):56-58.
69郑过灿,肖尚友,穆小静,等.自由基检测技术发展.广州化学,2006 ,31 (3):37-44.
70 Wojtaszewski JFP,Richter EA.Glucose utilization during exercise: inuence of endurance training[J].Acta Physiol Scand,1998,162:351-358.
71李秀娟,刘福英,张焕铃,等.复肝春六号对H22荷瘤小鼠的抑瘤及免疫的调节作用[J ].中国老年学杂志,2006(6):92-93.
72杨晓萍,罗祖友,吴谋成.油菜花粉多糖的制备及其对荷瘤小鼠的影响[J].食品科学,2005 ,26(12):202-204.
73张江巍,曹郁生,李海星等.乳酸菌抗氧化活性及检测方法[J].中国乳品工业,2005,33(9):53-56.
74 TERBLANCHE SE. the effects of exhaustive exercise on the activity leveles of catalasein various tissues of male and female rates[J]. cell biology international, 2000,23(11):749-753
75 V. A. Ilyukha.Superoxide Dismutase and Catalase in the Organs of Mammals of Different Ecogenesis[J] .Journal of Evolutionary Biochemistry and Physiology, 2001,37(3):241-245
76金鸣,李金荣,蔡亚欣等.红花水溶性成分抗氧化作用的研究[J].心肺血管病杂志,1998,17(4):227-279