茶薪菇液体培养胞外代谢产物制备及其抗氧化特性研究
|
摘要
为了研究茶薪菇(Agrocybe chaxingu Huang)液体培养胞外代谢产物体内外抗氧化特性,本研究以油茶枝与麦麸为主要原料,通过三个试验:比较不同营养基质的茶薪菇液体培养胞外代谢产物中多糖与酚类物质含量及其体外抗氧化特性,并研究对液体培养不同胞外代谢产物的分离制备技术(试验一);利用β-环状糊精包被的液体培养上清液,分析茶薪菇胞外代谢产物混合物对小鼠脏器指数与血清中抗氧化指标的影响(试验二);在此基础上,进一步分析茶薪菇胞外多糖与酚类代谢产物对小鼠生长性能、脏器指数以及血清中抗氧化指标的影响(试验三)。
分别以麦麸、油茶枝水提液培养基作为对照(CK-WB和CK-YC),与麦麸和油茶枝的混合水提液培养基(WB-YC)做比较;测定液体培养茶薪菇上清液中多糖与酚类物含量及其体外抗氧化特性;冷冻干燥醇析分离的胞外多糖与酚类产物,并分析制备产物的多糖与多酚物质含量。对茶薪菇的液体培养结果显示:上清液中,胞外多糖含量CK-WB组与WB-YC组高于CK-YC组(P<0.05),而酚类代谢物含量WB-YC组与CK-WB组高于CK-YC组(P<0.05);三组茶薪菇液体培养上清液对羟自由基的体外清除率顺序为:CK-WB > WB-YC > CK-YC(P<0.05)。
利用β-环状糊精(β-CD)包被,喷雾干燥制备茶薪菇胞外代谢产物混合粉;以4%油茶枝水提液组(CK-YC)为对照,对小鼠进行不同灌胃水平的比较试验。结果显示:灌胃不同剂量的4%油茶枝水提液(CK-YC)以及3%麦麸水提液+4%油茶枝水提液(WB-YC)的茶薪菇液体培养上清液制备胞外代谢产物,小鼠胸腺指数都较生理盐水灌胃组呈提高趋势;以灌胃600-800mg/kg·bw·d剂量的WB-YC组喷雾干燥制备产物,小鼠血清中SOD指标显著高于对照组(P<0.05)。
通过醇析分离,冷冻干燥制备茶薪菇液体培养胞外多糖与胞外酚类物质;分析其对小鼠灌胃试验的在体抗氧化特点。试验结果显示:与对照组相比,WB-YC组与CK-YC组的茶薪菇胞外酚类代谢物,能显著提高小鼠血清中的SOD活力(P<0.05),而其他各组的茶薪菇液体培养胞外多糖也不同程度地显示对提高小鼠血液中SOD活力的促进作用。同时,小鼠血液中MDA含量分析也显示,与对照组相比,不仅3%麦麸+4%油茶枝多糖组(P<0.05),其他各茶薪菇胞外代谢产物灌胃组均不同程度地降低血液中MDA含量。此外,分别灌胃茶薪菇液体培养胞外多糖与胞外酚类物,对小鼠脏器指数没有明显影响作用。
In this study, the water extracts of Camellia oleifera Abel branch powder and wheat bran were used in liquid medium for the submerged culture of Agrocybe chaxingu Huang. Three experiments were conducted, in which to compare the extracellular metabolites of A. chaxingu in polysaccharides and phenolic compounds from different culture medium and to study the techniques in the preparation for extracellular metabolites (Experiment 1); to evaluate the in vivo antioxidant effect of spray dried extracellular metabolites withβ-cyclodextrin (Experiment 2), freee dried extracellular polysaccharides and phenolic metabolites (Experiment 3), respectively, in mice. The growing performance, of mice and their viscera index were compared, the SOD and MDA content in serum were measured.
The water extract of wheat bran (CK-WB), Camellia oleifera Abel branches (CK-YC) and the mixture of the water extract of wheat bran and Camellia oleifera Abel branches (WB-YC) were compared as three culture media. The extracellular polysaccharide and phenolic compounds in the supernatant of submerged culture was separated with ethanol precipitation, then freeze dried. The content of polysaccharides and phenolic, and their in vitro antioxidant characters were determined. The results showed that the total polysaccharides in supernatant of subermerged culture was higher in the group of CK-WB and WB-YC than in CK-YC (P <0.05), while the total phenolic content was higher in WB-YC group followed by CK-WB than in CK-YC group (P <0.05). The clerarance rate of hydroxyl radical in Fention system rated in the order: CK-WB> WB-YC> CK-YC (P <0.05).
The extracellular metabolites of A. chaxingu were spray dried by use ofβ-cyclodextrin and were given to mice orally by gavage in different dosages. The results showed that the thymus index of mice was increased by giving spray dried submerged culture supernatant in all dosage level for CK-YC and WB-YC. In addition, for mice given 600-800mg/kgbw.d of spray dried supernatant of submerged culture, the SOD of mice serum was significantly higher in WB-YC than in CK-YC group (P <0.05).
Further more, the effect of phenolic and polysaccharide metabolits on improving antioxidation capacity of mice in vivo was evaluated. Comparing with mice given with saline, the extracellular phenolic metabolites of A. chaxingu in WB-YC and CK-YC groups increased SOD activity in mice serum significantly (P <0.05). The increases in SOD activity were also observed in mice give by polysaccharide metabolits. Moreover, comparing with mice given with saline, the decrease of MDA content in mice serum was found from various groups, especially in polysaccharide group of WB-YC (P<0.05).
分别以麦麸、油茶枝水提液培养基作为对照(CK-WB和CK-YC),与麦麸和油茶枝的混合水提液培养基(WB-YC)做比较;测定液体培养茶薪菇上清液中多糖与酚类物含量及其体外抗氧化特性;冷冻干燥醇析分离的胞外多糖与酚类产物,并分析制备产物的多糖与多酚物质含量。对茶薪菇的液体培养结果显示:上清液中,胞外多糖含量CK-WB组与WB-YC组高于CK-YC组(P<0.05),而酚类代谢物含量WB-YC组与CK-WB组高于CK-YC组(P<0.05);三组茶薪菇液体培养上清液对羟自由基的体外清除率顺序为:CK-WB > WB-YC > CK-YC(P<0.05)。
利用β-环状糊精(β-CD)包被,喷雾干燥制备茶薪菇胞外代谢产物混合粉;以4%油茶枝水提液组(CK-YC)为对照,对小鼠进行不同灌胃水平的比较试验。结果显示:灌胃不同剂量的4%油茶枝水提液(CK-YC)以及3%麦麸水提液+4%油茶枝水提液(WB-YC)的茶薪菇液体培养上清液制备胞外代谢产物,小鼠胸腺指数都较生理盐水灌胃组呈提高趋势;以灌胃600-800mg/kg·bw·d剂量的WB-YC组喷雾干燥制备产物,小鼠血清中SOD指标显著高于对照组(P<0.05)。
通过醇析分离,冷冻干燥制备茶薪菇液体培养胞外多糖与胞外酚类物质;分析其对小鼠灌胃试验的在体抗氧化特点。试验结果显示:与对照组相比,WB-YC组与CK-YC组的茶薪菇胞外酚类代谢物,能显著提高小鼠血清中的SOD活力(P<0.05),而其他各组的茶薪菇液体培养胞外多糖也不同程度地显示对提高小鼠血液中SOD活力的促进作用。同时,小鼠血液中MDA含量分析也显示,与对照组相比,不仅3%麦麸+4%油茶枝多糖组(P<0.05),其他各茶薪菇胞外代谢产物灌胃组均不同程度地降低血液中MDA含量。此外,分别灌胃茶薪菇液体培养胞外多糖与胞外酚类物,对小鼠脏器指数没有明显影响作用。
In this study, the water extracts of Camellia oleifera Abel branch powder and wheat bran were used in liquid medium for the submerged culture of Agrocybe chaxingu Huang. Three experiments were conducted, in which to compare the extracellular metabolites of A. chaxingu in polysaccharides and phenolic compounds from different culture medium and to study the techniques in the preparation for extracellular metabolites (Experiment 1); to evaluate the in vivo antioxidant effect of spray dried extracellular metabolites withβ-cyclodextrin (Experiment 2), freee dried extracellular polysaccharides and phenolic metabolites (Experiment 3), respectively, in mice. The growing performance, of mice and their viscera index were compared, the SOD and MDA content in serum were measured.
The water extract of wheat bran (CK-WB), Camellia oleifera Abel branches (CK-YC) and the mixture of the water extract of wheat bran and Camellia oleifera Abel branches (WB-YC) were compared as three culture media. The extracellular polysaccharide and phenolic compounds in the supernatant of submerged culture was separated with ethanol precipitation, then freeze dried. The content of polysaccharides and phenolic, and their in vitro antioxidant characters were determined. The results showed that the total polysaccharides in supernatant of subermerged culture was higher in the group of CK-WB and WB-YC than in CK-YC (P <0.05), while the total phenolic content was higher in WB-YC group followed by CK-WB than in CK-YC group (P <0.05). The clerarance rate of hydroxyl radical in Fention system rated in the order: CK-WB> WB-YC> CK-YC (P <0.05).
The extracellular metabolites of A. chaxingu were spray dried by use ofβ-cyclodextrin and were given to mice orally by gavage in different dosages. The results showed that the thymus index of mice was increased by giving spray dried submerged culture supernatant in all dosage level for CK-YC and WB-YC. In addition, for mice given 600-800mg/kgbw.d of spray dried supernatant of submerged culture, the SOD of mice serum was significantly higher in WB-YC than in CK-YC group (P <0.05).
Further more, the effect of phenolic and polysaccharide metabolits on improving antioxidation capacity of mice in vivo was evaluated. Comparing with mice given with saline, the extracellular phenolic metabolites of A. chaxingu in WB-YC and CK-YC groups increased SOD activity in mice serum significantly (P <0.05). The increases in SOD activity were also observed in mice give by polysaccharide metabolits. Moreover, comparing with mice given with saline, the decrease of MDA content in mice serum was found from various groups, especially in polysaccharide group of WB-YC (P<0.05).
引文
[1] Edwin N Frankel, Anne S Meyer. The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidants[J]. Sci Food and Agric, 2000, 80:1925-1941.
[2]杨冬梅.莲藕抗氧化特性研究[D].浙江大学博士论文,2007.
[3]徐清萍.镇江香醋抗氧化性研究[D].江南大学博士论文,2005.
[4]田金强,朱克瑞,李新明,等.阿魏菇多糖的抗氧化功能及其对果蝇寿命的影响[J].食品科学,2006,27(4):223-225.
[5] Lin JK, Lin CH, Liang YC, et al. Survey of catechins gallie acid and methylantions in green oolong Pu-erh and black teas[J]. Agrie Food Chem, 1998, 46:3635.
[6] Lin YL, Juan IM, Liang YC, et al. ComPosition of PolyPhenols in fresh tea leaves and Asso- ciations of their Oxygen-radical-absorbing capacity with antiProliferative actions in fibrobla- st cells[J].Agric Food Chem,1996,44:1387.
[7]郑德勇,安鑫南.植物抗氧化剂的研究概况与发展趋势[J].林产化学与工业,2004,24 (3): 114-117.
[8]李慎新,曹新志,钟俊波,等.几种中草药抗氧化性成分的还原能力及总酚含量比较研究[J].安徽农业科学,2008,36(29):12755—12756.
[9]潘英明,梁英,黄志清等.苏木、紫草等五种中草药抗氧化活性的研究[J].天然产物研究与开发,2004,(1):43-45.
[10]成喜雨,崔馨,刘春朝,等中草药抗氧化活性研究进展[J].天然产物研究与开发,2006, 18: 514-518.
[11] Khachik F,Beecher G,Smith J.Lutein,lycopene and their oxidative metabolics in hemo prevendon of cancer[J].Celle Biochem Supplement,1995,13(22):236-246.
[12]张名位,郭宝江,彭永宏等华南主要蔬菜抗氧化作用研究[J].华南师范大学学报,2003, 4:104-109.
[13]张佃志,方允中.某些蔬菜与水果的抗氧化活性观察[J].营养学报,1990,12(2):191-196.
[14]郭长江,韦京豫,杨继军等66种蔬菜、水果抗氧化活性的比较研究[J].营养学报,2003, 25(2):203-205.
[15]林秋实,陈吉棣.山楂及山楂黄酮预防大鼠脂质代谢紊乱的分子机制研究[J].营养学报, 2000,22:131-136.
[16]扬莉.西番莲果汁抗衰老作用的实验研究.营养学报,1995.17:438.
[17]柳中申.莉玫果抗衰老实验研究中医药学报,1990,4:2.
[18]沈恒胜,陈君琛,汤葆莎,等.栽培料纤维转化与食用菌酚类抗氧化营养的关系[J].福建农业学报,2007,22(4):337-340.
[19]沈恒胜,陈君琛,汤葆莎,等.生物去硅化作用对提高稻草降解率的处理效果探讨[J].中国农业科学,2006,39(2):406-411.
[20]沈恒胜,陈君琛,汤葆莎,等.食用菌生产的可持续发展与再生资源利用[J].中国食物与营养, 2005,10:18-20.
[21] Kmlo, Peter CK, Cheung. Antioxidant activity of extracts from the fruiting bodies of Agrocybe aegerita var alba[J].Food Chemistry,2005,89(4):533-539.
[22] Jeng-Leun Mau, Chieh-No, Chang, Shih-Jeng Huang. Antioxidant properties of methanolic extracts from Grifola frondosMorchella esculenta and Termitomyces albuminousus mycelia [J].Food Chemistry,2004,87:111-118.
[23] Aziz Turkoglu, Mehmet Emin, Duru Nazime Mercan.Antioxidant and antimicrobial activities of Laetiporus sulphureus(Bull.)Murrill[J].Food chemistry,2007,101:267-273.
[24]徐静娟,邬敏辰,陈屏.茶树菇多糖对荷瘤动物模型影响的研究[J].食品科学, 2005,26:246 -248.
[25]陈少英,王卫东,黎云.茶树菇多糖对小鼠免疫功能影响的初步研究[J].中国食用菌, 1999 ,24:34-36.
[26]张松,刘金庆,梅晓灯,等.茶树菇活性提取物抗氧化和延缓衰老作用的研究[J].营养学报, 2008,30(3):294-297.
[27]赵扬帆,林鸳缘,郑宝东.姬松茸酚类物质抗氧化活性的研究[J].农产品加工学刊,2008: 44-46.
[28]赵俊霞,袁广峰,徐瑞雅等.草菇培养物中粗三萜和黄酮含量及抗氧化抗肿瘤活性研究[J].南昌水专学报,2007,26(3):426-43.
[29]盛伟,吴萍,郭慧,等.白灵菇菌丝体胞内与胞外多糖体外抗氧化活性研究[J].中国林副特产,2008,3:8-10.
[30]李顺峰,张丽华,付娟妮,等.真姬菇子实体多糖体外抗氧化特性研究[J].西北农业学报, 2008 ,17(4):302-305.
[31]盖玉红.灵芝多糖抗氧化活性研究[M].吉林农业大学硕士论文,2007.
[32]王晓炜.大球盖菇营养成分分析、多糖提取分离及抗氧化作用研究[M].南京师范大学, 2007.
[33]沈侃,邵雪玲,邓小卫.平菇多糖清除O2及对红细胞膜自由基氧化的影响.氨基酸和生物资源[J].2002.24(2):19-21.
[34]李书倩,辛广,戴亚东,等.白灵菇多糖抗氧化性质的研究[J].鞍山师范学院学报, 2007, 9 (2):27-29.
[35]张连富,王莲芳,窦春霞,等.富硒食用菌多糖的体外抗氧化研究[J].天然产物研究与开发2007,19:1044-1047.
[36]史亚丽,杨立红,蔡德华,等.杏鲍菇多糖对力竭小鼠抗氧化、抗损伤的作用[J]体育学刊, 2005,12(1):56-58.
[37]余芳.富硒绿茶功能成分的抗氧化和抗肿瘤作用及其机理研究[D].南京:南京农业大学博士学位论文,2007.
[38]曹素芳,吴法先,李学锋,等.提高食用菌中硒含量的研究[J].中国食用菌,1992,11(5):10-12.
[39]姚敏.富硒灵芝的研究[J].上海大学学报,1996,2(5):513-519.
[40]谢必峰,林琳,施巧琴,等.灵芝菌的深层培养及富硒特性[J].食品与发酵工业,1996 (4): 54 -57.
[41]林琳,谢必峰,施巧琴,等.富硒金针菇的深层培养及其特性[J].中国食用菌,1997,16(2):36 -38.
[42]魏华,谢俊杰,傅金衡,等.灵芝富硒培养研究[J].食用菌,1996,18(3):7-8.
[43]邓超,邬敏辰.茶树菇深层发酵产物与子实体营养成分的分析[J].安徽农业科学,2007, 35 (5):27-28.
[44]程光宇,王晓炜,吴京燕,等.16种食用菌超氧物歧化酶(SOD)活性、热稳定性及同工酶的研究[J].南京师火学报,2006,29(4):96-100.
[45]吴国荣,陆长梅,周长芳,等.生理高温对佛州侧耳超氧物歧化酶的影响[J].菌物系统,2000, 19(3):37I-376.
[46]高文波,翁国.斌绿茶多酚抗氧化作用及其机制研究进展[J].国际药学研究杂志2009,36(5): 332-335.
[47]杨士钊,郑维发.影响桦褐孔菌培养菌丝体水解鞣质积累的因素[J].中草药,2007,38(12): 1874-1878.
[48]王晓炜,詹巍,陶明煊,等.大球盖菇营养成分、抗氧化活性物质分析.食用菌,2007,(6):62-63.
[49]中国营养学会.中国营养学会中国居民膳食营养素参考摄入量[M].北京中国轻工业出版社,2000,210-215.
[50]陆祖福,吴小南.慈菇营养素成分分析与抗氧化作用机理探讨[J].海峡预防医学杂志,2002, 8(1):50-51.
[51]徐红娟,邵红梅,孙书芹.高效液相色谱法测定香菇、猴头菇、金针菇3种食用菌中的7种水溶性维生素[J].三东化工,1998(5):581-582.
[52] Lucia R, Magdalena M, Daniela K, et al. Antiradical and antioxidant activities of alkaloids isolated from Mahonia aquifolium. Structural as Pects[J]. Bioorganie&Medicinalehemistry, 2004, 12(17):4709.
[53]方起程.天然药物化学研究[M].北京:中国协和医科大学出版社,2006.6.
[54]孔垂华,徐效华.有机物的分离和结构鉴定[M].北京:化学工业出版社,2003.7.
[55]梁振益,吴娟.薄荷提取物抗氧化性能的研究[J].中国油脂,2008,33(2):26-28.
[56]徐静.水果抗氧化活性与成分分析以及对衰老机体抗氧化功能的干预作用[D].南京:中国人民解放军军事医学科学院,博士论文.2007.
[57]吴俐.油茶枝液体培养茶薪菇代谢产物的抗氧化活性[D].福建:福建农林大学硕士论文, 2009.
[58] Riera E, Golas Y, Blanco A, et al. Mass transfer enhancement in supercritical fluids extraction by means of power ultrasound[J]. Ultrasonics Sonochemistry, 2004, 11 (3-4):241-244.
[59]项昭保,霍丹群,任绍光.超声波在中草药化学成分提取中的应用[J].自然杂志,2001, 23 (5):289-291.
[60]张磊.食用菌多糖超声浸提工艺及控制系统的研究[D].硕士论文.南京林业大学,2008.
[61]赖小玲,郑秀玲,郑浩文,等.超声波法提取茶薪菇粗多糖的工艺[J].食品与发酵工业,2007, 33(6):145-148.
[62]王厚廷,乔善义,杨明,等.超滤法提取六味地黄汤活性多糖的工艺研究[J].解放军药学学报, 2001,17(2):69-71.
[63]念保义,陈铭,王铮敏.超滤膜分离香菇多糖的研究[J].化学工业与工程技术,2003, 24 (4) 27 -29.
[64]潘仲巍,朱锦富,李惠芬,等.超滤膜分离技术提取茶多酚的研究[J].泉州师范学院学报, 2008,26(4):52-58.
[65]申进文,余海尤,霍云凤等斜生褐孔菌多糖组分的纯化及其生物活性研究[J].菌物学报2009,28(4):564-570.
[66]傅明辉,叶秀仪,郭秀苹,等.茶薪菇子实体多糖的分离纯化和抗氧化活性的测定[J].药物生物技术,2004,11(5):321-323.
[67]陈智毅,廖森泰,刘学铭,等.白金针菇子实体的单、双糖组成及其含量分析[J].食用菌学报. 2008,15(2):57-60.
[68]庞战军,周玫,陈瑗.自由基医学研究方法[M].北京:人民卫生出版社,2000.
[69]方允中,郑荣梁.自由基生物学的理论与应用[M].北京:科学出版社,2002.
[70]郑荣梁.自由基生命科学进展(第8集)[C].北京:原子能出版社,2001.
[71] Aruoma O I. Characterization of Drugs as Antioxidant Prophylactics[J]. Free Radical Biol Med,1996,20:675-705.
[72] Block G, Pattersen B, Subar A. Vegetables and Cancer Prevention:A Review of the Epidemi- ological Evidence[J]. Nute Cancer,1992,52:121-133.
[73] Duthie S J, MA A, Ross MA. Antioxidant Supplementation Decrease Oxidative DNA Dama- gein Human Lymphocytes[J]. Cancer Res,1996,56:1291-1295.
[74]刘小兵,朴建华.生物活性物质的抗氧化能力评价方法及其研究进展[J].中国食品卫生杂志,2008,20(5):440-444.
[75]崔英俊.滑菇多糖的抗衰老生物学效应[J].东北农业大学理学硕士学位论文,2002.
[76]幸浩洋,胡新珉,刘鸿莲,等.O3衰老小鼠模型的DNA损伤研究[J].华西医大学报, 2001, 32(2) :229-231.
[77]吴兰,娄宁,周玫,等.Ebselen对阿霉素致的小鼠亚急性心、肝、肾组织损伤的保护作用[J].基础医学与临床,1996,16(1):49-52.
[78]朱淑云,杨建雄,李发荣.连翘叶提取物对小鼠氧化损伤的保护作用[J].中药药理与临床, 2004,20(1):18-20.
[79]岳丽红,程广东,王长平,等.西藏灵菇酸奶对衰老模型小鼠抗氧化作用的研究[J].佳木斯大学学报,2008,26(3):430-432.
[80]张秀娟,杨姗姗,刘佰华,等蛹虫草提取物对H22小鼠抗氧化能力的影响[J].时珍国医国药,2008,19(4) : 943-944.
[81] Takeuchi N, Matsumiya K, Takahashi Y, et a1. Thiobarbituric acid reactive substances (TBARS) and lipid metabolism in atocopherol deficient rats [J]. Experimental Gerontology, 1977,12(1-2):63-68.
[82] Frenkel K, Karkoszka J, Glassma T, et al. Serum auto antibodies recognizing 5-hydroxymet -hyl- 2’-deoxyuridine anoxidized DNA base as biomarkers of cancer risk in women[J]. Cancer Epidemiol Biomarkers Prev, 1998(7):49-57.
[83] Zhao L, Zhao G, Hui B, et a1. Effect of Selenium on Increasing the Antioxidant Activity of Pprotein Extracts from a Selenium enriched Mushroom Species of the Ganoderma genus [J]. Journal of Food Science, 2006, 69(3):184-188.
[84] Chevion M, Berenshtein E, Stadtman E R. Human studies related to protein oxidation: protein carbonyl content as a marker of damage [J]. Free Radic Res, 2000,33(Supp1):99-108.
[85]黄志勇.茶薪菇人工培养成功中国食用菌,1995.(1):16.
[86]张松.食用菌学[M].广州:华南理工大学出版社,2000.
[87]杨月明,李美良.茶树菇栽培技术[M].北京:金盾出版社,2001.
[88]邓超,邬敏辰.茶树菇深层发酵产物与子实体营养成分的分析[J].安徽农业科学, 2007, 35(5):1342-1343.
[89]许旭萍,佘晨兴,李惠珍,等.柱状田头菇的栽培及生物化学研究概况[J].药物生物技术, 2003,10(2):117-120.
[90]袁广峰,徐瑞雅,张树斌,等茶薪菇培养物中粗三萜含量测定及抗氧化抗肿瘤活性研究[J]食用菌学报,2007,14(2):41-43.
[91]谢宝柱,张巧利,晁红雨.新型饲料添加剂茶多酚[J].饲料与营养,2005,(5):16-18.
[92]方允中,郑荣梁.自由基生物学的理论与应用[M].北京:科学出版社,2003:327-329.
[93] Cerutti RA.Prooxidant states and tumour promotion [J].Science, 1985, 227(4685):375.
[94]徐清萍.镇江香醋抗氧化性研究[D].江南大学博士论文,2005.
[95]王晓炜.大球盖菇营养成分分析、多糖提取分离及抗氧化作用研究[D].南京:南京师范大学硕士学位论文,2007.
[96]陈少英,王卫东,石鹤.茶树菇粗多糖体内抗氧化活性研究[J].湖北师范学院学报:自然科学版,2007,27(1):84-87.
[97]陈向东,刘晓雯,吴梧桐.灰树花多酚的提取和活性研究[J].食品与生物技术学报,2005,24 (4):26-31.
[98]沈恒胜,陈君琛等.栽培料纤维转化与食用菌酚类抗氧化营养的关系[J].福建农业学报, 2007,22(4):337-340.
[99]赵俊霞,袁广峰,徐瑞雅等.草菇培养物中粗三萜和黄酮含量及抗氧化抗肿瘤活性研究[J].南昌水专学报,2007,26(3):426-432.
[100]池玉杰,伊洪伟,刘智会.红平菇菌株H1的培养特性与营养成分分析[J].东北林业大学学报,2007,35(1):53-57.
[101]许旭萍,佘晨兴,李惠珍等.柱状田头菇的栽培及生物化学研究概况[J].药物生物技术, 2003,10(2):117-120.
[102]林琳,谢必峰,施巧琴等.富硒金针菇的深层培养及其特性[J].中国食用菌, 1997, 16 (2):36-38.
[103]谢必峰,林琳,施巧琴等.灵芝菌的深层培养及富硒特性[J].食品与发酵工业,1996 (4):54-57.
[104]魏华,谢俊杰,傅金衡等.灵芝富硒培养研究[J].食用菌,1996,18(3):7-8.
[105]暴增海,马桂珍,张建臣.茶薪菇的生物学特性及开发利用[J].食用菌,2007 (5):230-231.
[106]徐静娟,邬敏辰,陈屏.茶树菇多糖对荷瘤动物模型影响的研究[J].食品科学, 2005, 26: 246-248.
[107]陈少英,王卫东,黎云.茶树菇多糖对小鼠免疫功能影响的初步研究[J].中国食用菌, 24: 34-36.
[108] Na Y., Xin T, Ye X, et a1. CrystaLLization and preLiminary crystaLLographic studies of the recombinant antitumour Lectin from the edibLemushroom Agrocybe aegentita[J]. Biochim- Bfophys Acta, 2005,1751:209-212.
[109]王家鹏.灵芝多糖抗自由基与延缓衰老的实验研究[M]中医药大学硕士论文,2006.
[110] Ribeiro B, Rangel J, Valentao P, et al. Contents of carboxylic acids and two phenolics and antioxidant activity of dried portuguese wild edible mushrooms [J]. Agric Food Chem, 2006,54(22):8530-8537.
[111] Anh D H, Ullrich R, Benndorf D, et al. The coprophilous mushroom Coprinus radianse cretes a haloperoxidase that catalyzes aromatic peroxygenation [J]. Appl Environ Micro- biol, 2007,73(17):5477-5485.
[112]党西强,何小解,易著文等.微胶囊化儿茶素对阿霉素肾病大鼠总抗氧化能力的影响[J].食品科学,2007,28(1):299-301.
[113]陈梅香,张子德,马俊莲等微胶囊技术在抗氧化剂中的应用[J].河北农业大学学报,2002, (25):244-245.
[114]王俊强,顾震,马天贵等.微胶囊壁材的选择及其在食品工业中的应用[J].江西科学, 2008,26(2):242-244.
[115]陈志宏,齐聪儒,杨松鹤器官衰老机理和自由基学说[J].承德医学院学报,2003, 20 (2): 143-144.
[116]宋巧梅,唐方,谢肄聪.人参养荣汤对D-半乳糖致衰老小鼠抗氧化作用及脏器指数的影响[J].中国老年学杂志,2003,24(6):386-387.
[117]金宗濂.保健品的功能评价与开发[M].北京:中国轻工业出版社,2001.
[118]司传平.医学免疫学实验[M].北京:人民卫生出版社,1999.
[119]张松,刘金庆,梅晓灯等.茶树菇活性提取物抗氧化和延缓衰老作用的研究[J].营养学报,2008,30(3):294-296.
[120]胡庭俊,苏子杰,帅学宏.鬼臼多糖对免疫功能低下小鼠体内自由基水平的影响[J].安微农业科学,2009,37(7):2995-2996.
[121]罗燕,谷新利,徐小芳等.当归多糖对杂交肉鸡血清中SOD、MDA、GSH-Px的影响[J].黑龙江畜牧兽医科技版,2009,(7):105-106.
[122]高文波,翁国.斌绿茶多酚抗氧化作用及其机制研究进展[J].国际药学研究杂志2009,36 (5):332-335.
[123]朱磊,王振宇,黑木耳多糖对小鼠抗疲劳作用的研究[J].营养学报,2008,30(4):430-432.
[124]金莹,孙爱东.苹果多酚对小鼠的抗氧化作用研究[J].中国营养与食物,2006,(1):32-33.
[125]熊何健,周常义,郑新阳等.葡萄籽多酚对高脂膳食小鼠降血脂和抗氧化功能的影响[J].江西农业学报,2008,20(1):105-106.
[126]张本光. Agaricus blazei Murill降解麦麸阿拉伯木聚糖的营养学研究[D].福建:福建农林大学硕士论文, 2008.
[127]邓超,邬敏辰.茶树菇深层发酵条件的研究[J].江苏食品与发酵,2005(2):7-9.
[2]杨冬梅.莲藕抗氧化特性研究[D].浙江大学博士论文,2007.
[3]徐清萍.镇江香醋抗氧化性研究[D].江南大学博士论文,2005.
[4]田金强,朱克瑞,李新明,等.阿魏菇多糖的抗氧化功能及其对果蝇寿命的影响[J].食品科学,2006,27(4):223-225.
[5] Lin JK, Lin CH, Liang YC, et al. Survey of catechins gallie acid and methylantions in green oolong Pu-erh and black teas[J]. Agrie Food Chem, 1998, 46:3635.
[6] Lin YL, Juan IM, Liang YC, et al. ComPosition of PolyPhenols in fresh tea leaves and Asso- ciations of their Oxygen-radical-absorbing capacity with antiProliferative actions in fibrobla- st cells[J].Agric Food Chem,1996,44:1387.
[7]郑德勇,安鑫南.植物抗氧化剂的研究概况与发展趋势[J].林产化学与工业,2004,24 (3): 114-117.
[8]李慎新,曹新志,钟俊波,等.几种中草药抗氧化性成分的还原能力及总酚含量比较研究[J].安徽农业科学,2008,36(29):12755—12756.
[9]潘英明,梁英,黄志清等.苏木、紫草等五种中草药抗氧化活性的研究[J].天然产物研究与开发,2004,(1):43-45.
[10]成喜雨,崔馨,刘春朝,等中草药抗氧化活性研究进展[J].天然产物研究与开发,2006, 18: 514-518.
[11] Khachik F,Beecher G,Smith J.Lutein,lycopene and their oxidative metabolics in hemo prevendon of cancer[J].Celle Biochem Supplement,1995,13(22):236-246.
[12]张名位,郭宝江,彭永宏等华南主要蔬菜抗氧化作用研究[J].华南师范大学学报,2003, 4:104-109.
[13]张佃志,方允中.某些蔬菜与水果的抗氧化活性观察[J].营养学报,1990,12(2):191-196.
[14]郭长江,韦京豫,杨继军等66种蔬菜、水果抗氧化活性的比较研究[J].营养学报,2003, 25(2):203-205.
[15]林秋实,陈吉棣.山楂及山楂黄酮预防大鼠脂质代谢紊乱的分子机制研究[J].营养学报, 2000,22:131-136.
[16]扬莉.西番莲果汁抗衰老作用的实验研究.营养学报,1995.17:438.
[17]柳中申.莉玫果抗衰老实验研究中医药学报,1990,4:2.
[18]沈恒胜,陈君琛,汤葆莎,等.栽培料纤维转化与食用菌酚类抗氧化营养的关系[J].福建农业学报,2007,22(4):337-340.
[19]沈恒胜,陈君琛,汤葆莎,等.生物去硅化作用对提高稻草降解率的处理效果探讨[J].中国农业科学,2006,39(2):406-411.
[20]沈恒胜,陈君琛,汤葆莎,等.食用菌生产的可持续发展与再生资源利用[J].中国食物与营养, 2005,10:18-20.
[21] Kmlo, Peter CK, Cheung. Antioxidant activity of extracts from the fruiting bodies of Agrocybe aegerita var alba[J].Food Chemistry,2005,89(4):533-539.
[22] Jeng-Leun Mau, Chieh-No, Chang, Shih-Jeng Huang. Antioxidant properties of methanolic extracts from Grifola frondosMorchella esculenta and Termitomyces albuminousus mycelia [J].Food Chemistry,2004,87:111-118.
[23] Aziz Turkoglu, Mehmet Emin, Duru Nazime Mercan.Antioxidant and antimicrobial activities of Laetiporus sulphureus(Bull.)Murrill[J].Food chemistry,2007,101:267-273.
[24]徐静娟,邬敏辰,陈屏.茶树菇多糖对荷瘤动物模型影响的研究[J].食品科学, 2005,26:246 -248.
[25]陈少英,王卫东,黎云.茶树菇多糖对小鼠免疫功能影响的初步研究[J].中国食用菌, 1999 ,24:34-36.
[26]张松,刘金庆,梅晓灯,等.茶树菇活性提取物抗氧化和延缓衰老作用的研究[J].营养学报, 2008,30(3):294-297.
[27]赵扬帆,林鸳缘,郑宝东.姬松茸酚类物质抗氧化活性的研究[J].农产品加工学刊,2008: 44-46.
[28]赵俊霞,袁广峰,徐瑞雅等.草菇培养物中粗三萜和黄酮含量及抗氧化抗肿瘤活性研究[J].南昌水专学报,2007,26(3):426-43.
[29]盛伟,吴萍,郭慧,等.白灵菇菌丝体胞内与胞外多糖体外抗氧化活性研究[J].中国林副特产,2008,3:8-10.
[30]李顺峰,张丽华,付娟妮,等.真姬菇子实体多糖体外抗氧化特性研究[J].西北农业学报, 2008 ,17(4):302-305.
[31]盖玉红.灵芝多糖抗氧化活性研究[M].吉林农业大学硕士论文,2007.
[32]王晓炜.大球盖菇营养成分分析、多糖提取分离及抗氧化作用研究[M].南京师范大学, 2007.
[33]沈侃,邵雪玲,邓小卫.平菇多糖清除O2及对红细胞膜自由基氧化的影响.氨基酸和生物资源[J].2002.24(2):19-21.
[34]李书倩,辛广,戴亚东,等.白灵菇多糖抗氧化性质的研究[J].鞍山师范学院学报, 2007, 9 (2):27-29.
[35]张连富,王莲芳,窦春霞,等.富硒食用菌多糖的体外抗氧化研究[J].天然产物研究与开发2007,19:1044-1047.
[36]史亚丽,杨立红,蔡德华,等.杏鲍菇多糖对力竭小鼠抗氧化、抗损伤的作用[J]体育学刊, 2005,12(1):56-58.
[37]余芳.富硒绿茶功能成分的抗氧化和抗肿瘤作用及其机理研究[D].南京:南京农业大学博士学位论文,2007.
[38]曹素芳,吴法先,李学锋,等.提高食用菌中硒含量的研究[J].中国食用菌,1992,11(5):10-12.
[39]姚敏.富硒灵芝的研究[J].上海大学学报,1996,2(5):513-519.
[40]谢必峰,林琳,施巧琴,等.灵芝菌的深层培养及富硒特性[J].食品与发酵工业,1996 (4): 54 -57.
[41]林琳,谢必峰,施巧琴,等.富硒金针菇的深层培养及其特性[J].中国食用菌,1997,16(2):36 -38.
[42]魏华,谢俊杰,傅金衡,等.灵芝富硒培养研究[J].食用菌,1996,18(3):7-8.
[43]邓超,邬敏辰.茶树菇深层发酵产物与子实体营养成分的分析[J].安徽农业科学,2007, 35 (5):27-28.
[44]程光宇,王晓炜,吴京燕,等.16种食用菌超氧物歧化酶(SOD)活性、热稳定性及同工酶的研究[J].南京师火学报,2006,29(4):96-100.
[45]吴国荣,陆长梅,周长芳,等.生理高温对佛州侧耳超氧物歧化酶的影响[J].菌物系统,2000, 19(3):37I-376.
[46]高文波,翁国.斌绿茶多酚抗氧化作用及其机制研究进展[J].国际药学研究杂志2009,36(5): 332-335.
[47]杨士钊,郑维发.影响桦褐孔菌培养菌丝体水解鞣质积累的因素[J].中草药,2007,38(12): 1874-1878.
[48]王晓炜,詹巍,陶明煊,等.大球盖菇营养成分、抗氧化活性物质分析.食用菌,2007,(6):62-63.
[49]中国营养学会.中国营养学会中国居民膳食营养素参考摄入量[M].北京中国轻工业出版社,2000,210-215.
[50]陆祖福,吴小南.慈菇营养素成分分析与抗氧化作用机理探讨[J].海峡预防医学杂志,2002, 8(1):50-51.
[51]徐红娟,邵红梅,孙书芹.高效液相色谱法测定香菇、猴头菇、金针菇3种食用菌中的7种水溶性维生素[J].三东化工,1998(5):581-582.
[52] Lucia R, Magdalena M, Daniela K, et al. Antiradical and antioxidant activities of alkaloids isolated from Mahonia aquifolium. Structural as Pects[J]. Bioorganie&Medicinalehemistry, 2004, 12(17):4709.
[53]方起程.天然药物化学研究[M].北京:中国协和医科大学出版社,2006.6.
[54]孔垂华,徐效华.有机物的分离和结构鉴定[M].北京:化学工业出版社,2003.7.
[55]梁振益,吴娟.薄荷提取物抗氧化性能的研究[J].中国油脂,2008,33(2):26-28.
[56]徐静.水果抗氧化活性与成分分析以及对衰老机体抗氧化功能的干预作用[D].南京:中国人民解放军军事医学科学院,博士论文.2007.
[57]吴俐.油茶枝液体培养茶薪菇代谢产物的抗氧化活性[D].福建:福建农林大学硕士论文, 2009.
[58] Riera E, Golas Y, Blanco A, et al. Mass transfer enhancement in supercritical fluids extraction by means of power ultrasound[J]. Ultrasonics Sonochemistry, 2004, 11 (3-4):241-244.
[59]项昭保,霍丹群,任绍光.超声波在中草药化学成分提取中的应用[J].自然杂志,2001, 23 (5):289-291.
[60]张磊.食用菌多糖超声浸提工艺及控制系统的研究[D].硕士论文.南京林业大学,2008.
[61]赖小玲,郑秀玲,郑浩文,等.超声波法提取茶薪菇粗多糖的工艺[J].食品与发酵工业,2007, 33(6):145-148.
[62]王厚廷,乔善义,杨明,等.超滤法提取六味地黄汤活性多糖的工艺研究[J].解放军药学学报, 2001,17(2):69-71.
[63]念保义,陈铭,王铮敏.超滤膜分离香菇多糖的研究[J].化学工业与工程技术,2003, 24 (4) 27 -29.
[64]潘仲巍,朱锦富,李惠芬,等.超滤膜分离技术提取茶多酚的研究[J].泉州师范学院学报, 2008,26(4):52-58.
[65]申进文,余海尤,霍云凤等斜生褐孔菌多糖组分的纯化及其生物活性研究[J].菌物学报2009,28(4):564-570.
[66]傅明辉,叶秀仪,郭秀苹,等.茶薪菇子实体多糖的分离纯化和抗氧化活性的测定[J].药物生物技术,2004,11(5):321-323.
[67]陈智毅,廖森泰,刘学铭,等.白金针菇子实体的单、双糖组成及其含量分析[J].食用菌学报. 2008,15(2):57-60.
[68]庞战军,周玫,陈瑗.自由基医学研究方法[M].北京:人民卫生出版社,2000.
[69]方允中,郑荣梁.自由基生物学的理论与应用[M].北京:科学出版社,2002.
[70]郑荣梁.自由基生命科学进展(第8集)[C].北京:原子能出版社,2001.
[71] Aruoma O I. Characterization of Drugs as Antioxidant Prophylactics[J]. Free Radical Biol Med,1996,20:675-705.
[72] Block G, Pattersen B, Subar A. Vegetables and Cancer Prevention:A Review of the Epidemi- ological Evidence[J]. Nute Cancer,1992,52:121-133.
[73] Duthie S J, MA A, Ross MA. Antioxidant Supplementation Decrease Oxidative DNA Dama- gein Human Lymphocytes[J]. Cancer Res,1996,56:1291-1295.
[74]刘小兵,朴建华.生物活性物质的抗氧化能力评价方法及其研究进展[J].中国食品卫生杂志,2008,20(5):440-444.
[75]崔英俊.滑菇多糖的抗衰老生物学效应[J].东北农业大学理学硕士学位论文,2002.
[76]幸浩洋,胡新珉,刘鸿莲,等.O3衰老小鼠模型的DNA损伤研究[J].华西医大学报, 2001, 32(2) :229-231.
[77]吴兰,娄宁,周玫,等.Ebselen对阿霉素致的小鼠亚急性心、肝、肾组织损伤的保护作用[J].基础医学与临床,1996,16(1):49-52.
[78]朱淑云,杨建雄,李发荣.连翘叶提取物对小鼠氧化损伤的保护作用[J].中药药理与临床, 2004,20(1):18-20.
[79]岳丽红,程广东,王长平,等.西藏灵菇酸奶对衰老模型小鼠抗氧化作用的研究[J].佳木斯大学学报,2008,26(3):430-432.
[80]张秀娟,杨姗姗,刘佰华,等蛹虫草提取物对H22小鼠抗氧化能力的影响[J].时珍国医国药,2008,19(4) : 943-944.
[81] Takeuchi N, Matsumiya K, Takahashi Y, et a1. Thiobarbituric acid reactive substances (TBARS) and lipid metabolism in atocopherol deficient rats [J]. Experimental Gerontology, 1977,12(1-2):63-68.
[82] Frenkel K, Karkoszka J, Glassma T, et al. Serum auto antibodies recognizing 5-hydroxymet -hyl- 2’-deoxyuridine anoxidized DNA base as biomarkers of cancer risk in women[J]. Cancer Epidemiol Biomarkers Prev, 1998(7):49-57.
[83] Zhao L, Zhao G, Hui B, et a1. Effect of Selenium on Increasing the Antioxidant Activity of Pprotein Extracts from a Selenium enriched Mushroom Species of the Ganoderma genus [J]. Journal of Food Science, 2006, 69(3):184-188.
[84] Chevion M, Berenshtein E, Stadtman E R. Human studies related to protein oxidation: protein carbonyl content as a marker of damage [J]. Free Radic Res, 2000,33(Supp1):99-108.
[85]黄志勇.茶薪菇人工培养成功中国食用菌,1995.(1):16.
[86]张松.食用菌学[M].广州:华南理工大学出版社,2000.
[87]杨月明,李美良.茶树菇栽培技术[M].北京:金盾出版社,2001.
[88]邓超,邬敏辰.茶树菇深层发酵产物与子实体营养成分的分析[J].安徽农业科学, 2007, 35(5):1342-1343.
[89]许旭萍,佘晨兴,李惠珍,等.柱状田头菇的栽培及生物化学研究概况[J].药物生物技术, 2003,10(2):117-120.
[90]袁广峰,徐瑞雅,张树斌,等茶薪菇培养物中粗三萜含量测定及抗氧化抗肿瘤活性研究[J]食用菌学报,2007,14(2):41-43.
[91]谢宝柱,张巧利,晁红雨.新型饲料添加剂茶多酚[J].饲料与营养,2005,(5):16-18.
[92]方允中,郑荣梁.自由基生物学的理论与应用[M].北京:科学出版社,2003:327-329.
[93] Cerutti RA.Prooxidant states and tumour promotion [J].Science, 1985, 227(4685):375.
[94]徐清萍.镇江香醋抗氧化性研究[D].江南大学博士论文,2005.
[95]王晓炜.大球盖菇营养成分分析、多糖提取分离及抗氧化作用研究[D].南京:南京师范大学硕士学位论文,2007.
[96]陈少英,王卫东,石鹤.茶树菇粗多糖体内抗氧化活性研究[J].湖北师范学院学报:自然科学版,2007,27(1):84-87.
[97]陈向东,刘晓雯,吴梧桐.灰树花多酚的提取和活性研究[J].食品与生物技术学报,2005,24 (4):26-31.
[98]沈恒胜,陈君琛等.栽培料纤维转化与食用菌酚类抗氧化营养的关系[J].福建农业学报, 2007,22(4):337-340.
[99]赵俊霞,袁广峰,徐瑞雅等.草菇培养物中粗三萜和黄酮含量及抗氧化抗肿瘤活性研究[J].南昌水专学报,2007,26(3):426-432.
[100]池玉杰,伊洪伟,刘智会.红平菇菌株H1的培养特性与营养成分分析[J].东北林业大学学报,2007,35(1):53-57.
[101]许旭萍,佘晨兴,李惠珍等.柱状田头菇的栽培及生物化学研究概况[J].药物生物技术, 2003,10(2):117-120.
[102]林琳,谢必峰,施巧琴等.富硒金针菇的深层培养及其特性[J].中国食用菌, 1997, 16 (2):36-38.
[103]谢必峰,林琳,施巧琴等.灵芝菌的深层培养及富硒特性[J].食品与发酵工业,1996 (4):54-57.
[104]魏华,谢俊杰,傅金衡等.灵芝富硒培养研究[J].食用菌,1996,18(3):7-8.
[105]暴增海,马桂珍,张建臣.茶薪菇的生物学特性及开发利用[J].食用菌,2007 (5):230-231.
[106]徐静娟,邬敏辰,陈屏.茶树菇多糖对荷瘤动物模型影响的研究[J].食品科学, 2005, 26: 246-248.
[107]陈少英,王卫东,黎云.茶树菇多糖对小鼠免疫功能影响的初步研究[J].中国食用菌, 24: 34-36.
[108] Na Y., Xin T, Ye X, et a1. CrystaLLization and preLiminary crystaLLographic studies of the recombinant antitumour Lectin from the edibLemushroom Agrocybe aegentita[J]. Biochim- Bfophys Acta, 2005,1751:209-212.
[109]王家鹏.灵芝多糖抗自由基与延缓衰老的实验研究[M]中医药大学硕士论文,2006.
[110] Ribeiro B, Rangel J, Valentao P, et al. Contents of carboxylic acids and two phenolics and antioxidant activity of dried portuguese wild edible mushrooms [J]. Agric Food Chem, 2006,54(22):8530-8537.
[111] Anh D H, Ullrich R, Benndorf D, et al. The coprophilous mushroom Coprinus radianse cretes a haloperoxidase that catalyzes aromatic peroxygenation [J]. Appl Environ Micro- biol, 2007,73(17):5477-5485.
[112]党西强,何小解,易著文等.微胶囊化儿茶素对阿霉素肾病大鼠总抗氧化能力的影响[J].食品科学,2007,28(1):299-301.
[113]陈梅香,张子德,马俊莲等微胶囊技术在抗氧化剂中的应用[J].河北农业大学学报,2002, (25):244-245.
[114]王俊强,顾震,马天贵等.微胶囊壁材的选择及其在食品工业中的应用[J].江西科学, 2008,26(2):242-244.
[115]陈志宏,齐聪儒,杨松鹤器官衰老机理和自由基学说[J].承德医学院学报,2003, 20 (2): 143-144.
[116]宋巧梅,唐方,谢肄聪.人参养荣汤对D-半乳糖致衰老小鼠抗氧化作用及脏器指数的影响[J].中国老年学杂志,2003,24(6):386-387.
[117]金宗濂.保健品的功能评价与开发[M].北京:中国轻工业出版社,2001.
[118]司传平.医学免疫学实验[M].北京:人民卫生出版社,1999.
[119]张松,刘金庆,梅晓灯等.茶树菇活性提取物抗氧化和延缓衰老作用的研究[J].营养学报,2008,30(3):294-296.
[120]胡庭俊,苏子杰,帅学宏.鬼臼多糖对免疫功能低下小鼠体内自由基水平的影响[J].安微农业科学,2009,37(7):2995-2996.
[121]罗燕,谷新利,徐小芳等.当归多糖对杂交肉鸡血清中SOD、MDA、GSH-Px的影响[J].黑龙江畜牧兽医科技版,2009,(7):105-106.
[122]高文波,翁国.斌绿茶多酚抗氧化作用及其机制研究进展[J].国际药学研究杂志2009,36 (5):332-335.
[123]朱磊,王振宇,黑木耳多糖对小鼠抗疲劳作用的研究[J].营养学报,2008,30(4):430-432.
[124]金莹,孙爱东.苹果多酚对小鼠的抗氧化作用研究[J].中国营养与食物,2006,(1):32-33.
[125]熊何健,周常义,郑新阳等.葡萄籽多酚对高脂膳食小鼠降血脂和抗氧化功能的影响[J].江西农业学报,2008,20(1):105-106.
[126]张本光. Agaricus blazei Murill降解麦麸阿拉伯木聚糖的营养学研究[D].福建:福建农林大学硕士论文, 2008.
[127]邓超,邬敏辰.茶树菇深层发酵条件的研究[J].江苏食品与发酵,2005(2):7-9.