交泰丸方药的半仿生提取法实验研究
摘要
目的：为交泰丸剂改研究优选提取工艺；进一步验证半仿生提取法(简称SBE法)的科学性和实用性。方法：先以小檗碱、肉桂酸、总生物碱、挥发油、干浸膏含量为指标，用均匀设计优选出交泰丸方药的SBE法工艺条件；再将方药组合成2组，用半仿生提取法提取，提取液作指标成分的含量测定及甲醇精制液的薄层色谱、挥发油的气相色谱比较，优选出交泰丸方药SBE药材的最佳组合方式；然后对该方药最佳组合的SBE液作不同醇沉浓度的成分比较，优选出半仿生提取醇沉法(SBAE法)的最佳醇沉浓度；在此基础上作SBE法、水提取法(WE法)、SBAE法、水提取醇沉法(WAE法)4种方法提取液的成分比较及SBE液与WE液的镇静作用比较。结果：交泰丸方药SBE的工艺条件为：第1煎用水以HCL调至pH2.20，第2、3煎用水依次以NaOH调至pH6.50，8.70；煎煮时间依次为170min，80min，40min；方药提取时以黄连、肉桂分别提取为佳；4种方法提取液5个指标成分的综合评价值为：SBE液＞WE液＞SBAE液＞WAE液；SBE液较WE液的镇静作用明显增强。结论：交泰丸方药成分提取时以SBE法为佳。本研究进一步验证了SBE法的科学性和实用性，完善了中药复方剂改用SBE法的基本研究模式，并为中药药对的研究提供了借鉴。
Objective: Optimizing the extract technology in the modification of the formula of Jiaotai Pill and further verifying the correctness and practicality of semi-bionic extraction(SBE). Methods: At first, optimizing the SBE technical conditions for Jiaotai Pill ingredients through even design while berberine, cinnamic acid, total alkaloids, volatile oil and dry extract were adopted as indexs; Then, dividing the ingredients into 2 combinations and extracting by the SBE method respectively, determining the contents of index components in the each extract, comparing the TLC of absolute methanol solution and the GC of volatile oil, and making out the SBE s best ingredients combination For the formula of Jiaotai Pill. The SBE extract of the optimized ingredients combination was put to further comparison of components in different conditions of alcohol precipitating concentrations, confirming the most ideal alcohol precipitating concentration of the semi-bionic extraction by precipitation with alcohol (SBAE) for the formula. On this base, comparison of components were conducted over the four extracts derived from four different kinds of extraction methods that are the SBE, the water extraction(WE), SBAE, and the water extraction by precipitation with alcohol (WAE), and the mitigtive functions of the extracts of SBE and WE were compared. Results: The technical condition of SBE for the formula of Jiaotai Pill was: The pH value in first extraction was adjusted to 2.20 by the use of HCL solution. And then the pH values in the second and third extraction should be adjusted to 6. 50 and 8.70 by the use of NaOH solution, the durations of
each section were 170min, 80min and 40min, respectively. it is better to extract Rhizoma Coptidis and Cortex Cinnamomi seperately. The comprehensive values assessed by the contents of 5 index components in the four extracts were : SBE >WE>SBAE>WAE. The mitigtive function of SBE extract is obviously more effective than that of WE. Conclusion: SBE method is the best among the four methods in the extraction of Jiaotai Pill components. The study firmly verifies that the SBE method is correct and practical, consummates the basic study model of Traditional Medicine compound s modification by using the SBE method , provides some experiences for Traditional Medicine s matching study.
Objective: Optimizing the extract technology in the modification of the formula of Jiaotai Pill and further verifying the correctness and practicality of semi-bionic extraction(SBE). Methods: At first, optimizing the SBE technical conditions for Jiaotai Pill ingredients through even design while berberine, cinnamic acid, total alkaloids, volatile oil and dry extract were adopted as indexs; Then, dividing the ingredients into 2 combinations and extracting by the SBE method respectively, determining the contents of index components in the each extract, comparing the TLC of absolute methanol solution and the GC of volatile oil, and making out the SBE s best ingredients combination For the formula of Jiaotai Pill. The SBE extract of the optimized ingredients combination was put to further comparison of components in different conditions of alcohol precipitating concentrations, confirming the most ideal alcohol precipitating concentration of the semi-bionic extraction by precipitation with alcohol (SBAE) for the formula. On this base, comparison of components were conducted over the four extracts derived from four different kinds of extraction methods that are the SBE, the water extraction(WE), SBAE, and the water extraction by precipitation with alcohol (WAE), and the mitigtive functions of the extracts of SBE and WE were compared. Results: The technical condition of SBE for the formula of Jiaotai Pill was: The pH value in first extraction was adjusted to 2.20 by the use of HCL solution. And then the pH values in the second and third extraction should be adjusted to 6. 50 and 8.70 by the use of NaOH solution, the durations of
each section were 170min, 80min and 40min, respectively. it is better to extract Rhizoma Coptidis and Cortex Cinnamomi seperately. The comprehensive values assessed by the contents of 5 index components in the four extracts were : SBE >WE>SBAE>WAE. The mitigtive function of SBE extract is obviously more effective than that of WE. Conclusion: SBE method is the best among the four methods in the extraction of Jiaotai Pill components. The study firmly verifies that the SBE method is correct and practical, consummates the basic study model of Traditional Medicine compound s modification by using the SBE method , provides some experiences for Traditional Medicine s matching study.
引文
[1] 张坤瑞，刘耀，高军．交泰丸治疗失眠证149例临床研究[J]．实用中医药杂志，1994，10(4)：12．
[2] 高伯正．交泰丸释名[J]．光明中医杂志，1996，(6)：6．
[3] 附属第一医院中医科．交泰丸治疗50例神经官能症的疗效观察[J]．北京医学院学报，1975(3)：162．
[4] 陈元和．交泰丸治疗失眠证23例[J]．实用中西医结合杂志，1992，5(11)：695．
[5] 张坤瑞，刘耀，高军．交泰丸治疗失眠证149例临床研究[J]．实用中医药杂志，1994，10(4)：12．
[6] 姜静娴，孙固祖．安眠外贴灵的临床观察[J]．中国中医基础医学杂志(增刊)，1997，(3)：87．
[7] 何永田，边可陶，袁福茹．交泰丸加味治疗快速型心律失常68例[J]浙江中医杂志，1992，27(9)：386．
[8] 张文华．交泰丸加味治疗室性早搏68例[J]．广西中医药，1994，17(4)：151．
[9] 胡兆满．黄连伍肉桂治疗口腔疾病[J]．中医杂志．1989，24(4)：181．
[10] 苗明三，李新华，许风云，等．交泰丸对中枢神经系统的影响[J]．中药药理与临床，1991，7(1)：26．
[11] 许凤云，孙曙光，苗明三，等．交泰丸对心血管系统的影响[J]．河南中医，1992，12(6)：266．
[12] 周洪雷，魏璐雪，杨东辉，等．高效毛细管电泳法测定黄连肉桂配伍前后主要生物碱的含量[J]．中国中药杂志，1999，24(5)：308．
[13] 徐艳春，魏璐雪，王亚丽．高效液相色谱法测定黄连配伍肉桂前后肉桂酸的含量[J]．中国中药杂志，2001，26(1)：47．
[14] 张兆旺，孙秀梅．试论“半仿生提取法”制备中药口服制剂[J]．中国中药杂志，1995，20(11)：670．
[15] 张瑞亭，张兆旺，孙秀梅．思维方式的转换与中药“半仿生提取法”[J]．中国中药杂志，1997，22(9)：542．
[16] 孙秀梅，张兆旺，郭婕，等．芍甘止痛颗粒剂4种提取方法的成分比较．中成药，1996，
18(11)：3．
[17] 孙秀梅，张兆旺，丁菊英，等．寒痛定泡腾冲剂4种提取方法的比较．中国中药杂志，1996，21(3)：159．
[18] 孙秀梅，张兆旺，张学兰，等．四神茶剂4种提取方法的成分比较．中国中药杂志，1997，22(6)：349．
[19] 张学兰，张兆旺，孙秀梅，等．四神茶剂4种提取方法的药效学比较．中国中药杂志，1997，(增刊)：201．
[20] 张兆旺，孙秀梅，王蕾，等．麻杏石甘汤2种提取方法的成分比较．中国中药杂志，1997，22(7)：413．
[21] 张兆旺，王英姿．当归苦参丸2种方法提取液的成分比较．中国中药杂志，1999，24(12)：734．
[22] 孙秀梅，张兆旺，修彦凤．参附汤2种方法提取液的成分比较．中成药(电子版)，1999．
[23] 吕青涛，张兆旺，孙秀梅，等．乙肝颗粒剂4种方法提取液的成分比较．中成药 2000，22(11)：752．
[24] 孙秀梅，张兆旺，卢连华，等．黄连解毒颗粒剂4种提取方法的成分比较．中国中药杂志，1996，21(8)：474．
[25] 张兆旺，王英姿，孙秀梅．当归苦参丸2种方法提取液的成分比较．中国中药杂志，1999，24(12)：734．
[26] 北京医学院，北京中医学院．中草药成分化学[M]．北京：人民出版社．1981．125．
[27] 中华人民共和国药典(一部)[S]．北京：化学工业出版社，2000．105．
[2] 高伯正．交泰丸释名[J]．光明中医杂志，1996，(6)：6．
[3] 附属第一医院中医科．交泰丸治疗50例神经官能症的疗效观察[J]．北京医学院学报，1975(3)：162．
[4] 陈元和．交泰丸治疗失眠证23例[J]．实用中西医结合杂志，1992，5(11)：695．
[5] 张坤瑞，刘耀，高军．交泰丸治疗失眠证149例临床研究[J]．实用中医药杂志，1994，10(4)：12．
[6] 姜静娴，孙固祖．安眠外贴灵的临床观察[J]．中国中医基础医学杂志(增刊)，1997，(3)：87．
[7] 何永田，边可陶，袁福茹．交泰丸加味治疗快速型心律失常68例[J]浙江中医杂志，1992，27(9)：386．
[8] 张文华．交泰丸加味治疗室性早搏68例[J]．广西中医药，1994，17(4)：151．
[9] 胡兆满．黄连伍肉桂治疗口腔疾病[J]．中医杂志．1989，24(4)：181．
[10] 苗明三，李新华，许风云，等．交泰丸对中枢神经系统的影响[J]．中药药理与临床，1991，7(1)：26．
[11] 许凤云，孙曙光，苗明三，等．交泰丸对心血管系统的影响[J]．河南中医，1992，12(6)：266．
[12] 周洪雷，魏璐雪，杨东辉，等．高效毛细管电泳法测定黄连肉桂配伍前后主要生物碱的含量[J]．中国中药杂志，1999，24(5)：308．
[13] 徐艳春，魏璐雪，王亚丽．高效液相色谱法测定黄连配伍肉桂前后肉桂酸的含量[J]．中国中药杂志，2001，26(1)：47．
[14] 张兆旺，孙秀梅．试论“半仿生提取法”制备中药口服制剂[J]．中国中药杂志，1995，20(11)：670．
[15] 张瑞亭，张兆旺，孙秀梅．思维方式的转换与中药“半仿生提取法”[J]．中国中药杂志，1997，22(9)：542．
[16] 孙秀梅，张兆旺，郭婕，等．芍甘止痛颗粒剂4种提取方法的成分比较．中成药，1996，
18(11)：3．
[17] 孙秀梅，张兆旺，丁菊英，等．寒痛定泡腾冲剂4种提取方法的比较．中国中药杂志，1996，21(3)：159．
[18] 孙秀梅，张兆旺，张学兰，等．四神茶剂4种提取方法的成分比较．中国中药杂志，1997，22(6)：349．
[19] 张学兰，张兆旺，孙秀梅，等．四神茶剂4种提取方法的药效学比较．中国中药杂志，1997，(增刊)：201．
[20] 张兆旺，孙秀梅，王蕾，等．麻杏石甘汤2种提取方法的成分比较．中国中药杂志，1997，22(7)：413．
[21] 张兆旺，王英姿．当归苦参丸2种方法提取液的成分比较．中国中药杂志，1999，24(12)：734．
[22] 孙秀梅，张兆旺，修彦凤．参附汤2种方法提取液的成分比较．中成药(电子版)，1999．
[23] 吕青涛，张兆旺，孙秀梅，等．乙肝颗粒剂4种方法提取液的成分比较．中成药 2000，22(11)：752．
[24] 孙秀梅，张兆旺，卢连华，等．黄连解毒颗粒剂4种提取方法的成分比较．中国中药杂志，1996，21(8)：474．
[25] 张兆旺，王英姿，孙秀梅．当归苦参丸2种方法提取液的成分比较．中国中药杂志，1999，24(12)：734．
[26] 北京医学院，北京中医学院．中草药成分化学[M]．北京：人民出版社．1981．125．
[27] 中华人民共和国药典(一部)[S]．北京：化学工业出版社，2000．105．