优酪酸、植酸酶及霉毒素吸附剂在猪生产中的应用
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
为解决仔猪腹泻、粪便氮磷污染及饲料黄曲霉菌污染对养猪业的影响,本课题在贵州台农种养殖公司龙里种猪场内进行了断奶仔猪日粮添加优酪酸、生长仔猪日粮中添加植酸酶、繁殖母猪日粮添加霉毒素吸附剂试验。
     试验一:用48头28日龄,体重相近、健康的断奶仔猪随机分为对照组、试验Ⅰ、Ⅱ、Ⅲ组4个组每组12头,试验组日粮中分别添加0.5%、1%、1.5%优酪酸,进行了28天饲养试验。结果表明:对照组、试验Ⅰ、Ⅱ、Ⅲ组的平均日增重分别为0.376kg、0.398kg、0.410kg、0.409kg,其中以试验Ⅱ组的平均日增重最高,分别比对照组高9.04%(p<0.05)、比试验Ⅰ组高3.02%、比试验Ⅲ组高0.24%;料肉比分别为1.75:1、1.70:1、1.67:1、1.67:1,其中以试验Ⅱ组的料肉比最低,分别比对照组低4.57%(p<0.05),比试验Ⅰ组低1.76%、比试验Ⅲ组基本持平;仔猪腹泻发生率分别为66.67%、33.33%、16.67%、33.33%,其中以试验Ⅱ组腹泻发生率最低。
     试验二:用96头体重30Kg左右杜×长×大三元杂仔猪,随机分为A(对照组)、B、C、D组4个组每组24头,试验组分别在日粮中添加250U/kg、500U/kg、750U/kg植酸酶,进行了42d饲养试验,另取12头分4组每组3头进行消化试验。结果表明:A、B、C、D组的平均日增重分别为0.75kg、0.765kg、0.78kg、0.77kg,C组试验组的平均日增重分别比对照组高1.05%(P>0.05),比B组高4.09%(P<0.05),比D组高2.64%(P>0.05),猪粪中CP分别降低了5.45%(P>0.05),8.26%(P<0.05)和8.15%(P<0.05),钙含量分别降低了1.30%(P>0.05),4.56%(P<0.01)和3.58%(P>0.01);磷含量分别降低了6.09%(P<0.05),9.57%(P<.01)和6.96%(P<0.01)。
     试验三:用32头健康、体况良好5胎次大约克经产母猪,随机分为对照组、试验1,2,3组4个组,每组8头,试验组在分别日粮中添加0.15%、0.3%、0.45%霉毒素吸附剂,分妊娠后期(86 d-分娩)、哺乳期(0-28 d)2个阶段进行了饲养试验。结果表明,对照组和3个试验组产仔数分别为10.25、10.56、11.5、11.10,试验2组产仔数最高;仔猪初生窝重分别为11.38 kg、13.19 kg、15.76kg、14.87 kg,其中试验2组仔猪初生窝重最高;4个组的初生个体重依序分别为1.30 kg、1.35 kg、1.44 kg、1.41 kg,分别比对照组高0.05 kg、0.14 kg、0.11 kg,4个组断奶个体重依序分别为6.68kg、6.88kg、7.71kg、7.53kg,3个试验组都比对照组高,4个组母猪的受胎率依序分别为78.25%、82.13%、85.76%、84.98%,试验2组的受胎率最高;4个组的流产率依序分别为4.65%、2.55%、1.82%、2.09%,对照组的流产率最高,试验2组流产率最低,整个试验期间,母猪的总耗料量和体重损失差异不显著(P>0.05)。
     从而认为,在仔猪日粮中添加优酪酸可以提高哺乳仔猪的增重、饲料转化效率、预防仔猪腹泻,以添加1%为宜;添加植酸酶可以明显减少生长猪粪便中蛋白质、钙、磷等的排泄量,对生长和饲料转化效率、降低饲养成本有一定作用,以添加500U/kg植酸酶效果最佳;在母猪日粮中添加霉毒素吸附剂可以保证母猪繁殖能力,降低流产率、提高初生窝重具有明显作用,以添加0.3%霉毒素吸附剂效果最为明显。此三项技术在养猪生产中具有很高的推广应用价值。
The experiment was located at Longli pig breeding farm of Tailong company about optimal butyric acid added with piglets diet,weaned piglets diet added with phytase and sow fertility diet added with aflatoxin adsorbent,in order to solve the effects of piglet diarrhea、nitrogen and phosphorus pollution from feces and aspergillus flavus pollution of feed on pig industry.
     We choosed 48 weaned piglets of 28 days old,which are healthy and weight close for the experiment 1,there are four groups and 12 replicates per group,and four levels of optimal butyric acid(CK,0.5%,1%,1.5%) added to their diet for 28 d.The results indicated that the average daily gain of weaned piglets were 0.376kg in groupⅠ、0.398kg in groupⅡ、0.410kg in groupⅢand 0.409kg in group 3,the groupⅡhad the greatest average daily gain in the four groups,9.04%(p<0.05 )higher than CK, 3.02%higher than group 1 and 0.24%higher than group 4;feed conversion ratio of CK、groupⅠ、groupⅡand groupⅢwere 1.75:1、1.70:1、1.67:1、1.67:1,the groupⅡhad the lowest feed conversion ratio in the four groups,4.57%(p<0.05)lower than CK,1.76%lower than groupⅠand equal to groupⅢ;diarrhea incidence of CK、groupⅠ、groupⅡand groupⅢwere 66.67%、33.33%、16.67%、33.33%,and the groupⅡhad the lowest diarrhea incidence in the four groups.
     There are 96 Duroc×Landrace×Yorkshire three-breed cross piglets of 30kg weight for the experiment 2,they were divided to four groups and 24 rcplicatcs per group,and four levels phytase(CK、250U/kg、500U/kg、750U/kg) added to their diet for 42 d,additional 12 DLY three-breed cross piglets for digestion trial.The result indicated that the average daily gain was 0.75kg、0.765kg、0.78kg and 0.77kg respcctiely for A、group B、group C and groupD the average daily gain of groupA、groupB and group C was respectiely 1.05%(P>0.05),4.09(P<0.05),2.64%(P>0.05) higher than groupA,CP in fece of groupB、group C and group D was respectiely 5.45%(P>0.05),8.26%(P<0.05) and 8.15%(P<0.05) lower than groupA,Ca was respectiely 1.30%(P>0.05),4.56%(P<0.01) and 3.58%(P>0.01) lower than groupA, P was respectiely 6.09%(P<0.05),9.57%(P<.01) and 6.96%(P<0.01) lower than groupA.
     There are 32 healthy Yorkshire multiparous sows of 5 fetal times for the experiment 3,they were divided to four groups and 8 replicates per group,and four levds penicillic toxin adsorbent(CK、0.15%、0.3%、0.45%) added to their diet,the feeding was in two phases of late pregnancy(86 d to delivery) and lactation period(0 d to 28 d).The result shows that litter size was 10.25、10.56、11.5 and 11.10 respectiely for CK、group 1、group 2 and group 3,and group 2 had the greatest litter size,litter weight of piglet of CK、group 1、group2、group 3 was respectively 11.38 kg、13.19 kg、15.76 kg、14.87 kg,and group 2 had the highest litter weight of piglet,piglets birth weight was respectively 1.30 kg、1.35 kg、1.44 kg、1.41 kg,the three treat groups are all higher than CK;conception rate was respectively 78.25%、82.13%、85.76%、84.98%,and group 2 had the highest conception rate in the four groups;abortion rate was respectively 4.65%、2.55%、1.82%、2.09%,CK had the highest abortion rate.The total feed consumption and body weight loss of sows had not significant difference(P>0.05) during the experiment period.
     Therefore,with the application of optimal butyric acid in feed,the weight gain of suckling piglets and feed conversion ratio increased,piglet diarrhea decreased, particularly,it had a best effect with 1%optimal butyric acid in feed;the CP、Ca and P in fece of growing pigs decreased,the gain and feed conversion rate improved and the feed cost reduced with the application of phytase,the efficiency was greatest when phytase in 500U/kg concentration;The application of aflatoxin adsorbent in sows diet can raise the litter weight,of piglet,decrease abortion rate,it had a best effect with the addition of 0.3%penicillic toxin adsorbent.the
引文
[1]2006年贵阳市统计年鉴.
    [2]白正辉.黄曲霉毒素致猪原发性肝细胞癌的诊断[J].中国动物检疫,2001,18(10):32-32.
    [3]柏巧明,何波,陈建华,等.魔芋属药植资源的研究湖北农学院学报[J],2000,20(3):213-217.
    [4]宝英,朱晓慧,刘佳,酪酸菌对动物肠道致病菌体外拮抗作用的研究[J].生物技术,2005,15(1):37-39.
    [5]崔汉钧,和智明,贾成禹魔芋葡甘露聚糖固定化环糊精葡基转移酶的研究天然产物研究与开发[J].饲料土业,1993,5(3):48-54.
    [6]董银果,徐恩波.中国猪肉安全问题及对策[J].西南农业大学学报,2005,3(3):47-50.
    [7]樊哲炎.HACCP体系在无公害养猪上的应用和推广研究[D].浙江:浙江大学,2004.6.
    [8]范兴良译.断奶仔猪日粮以磷酸为基础的酸化剂[J].国外畜牧学-猪与禽,1995(3):10.
    [9]付德顺.植酸酶在配合饲料中的应用[J].河南畜牧兽医,2000,27:27-28.
    [10]顾公望和周汉高.黄曲霉毒素与肝细胞癌分子生物学研究[J].浙江肿瘤,1998 4(4):265-267
    [11]郭松林.植酸酶营养学研究近况[J].上海饲料,2000,(5):22-25.
    [12]国家技术监督局.饲料卫生标准及检测方法[M].北京:中国标准出版社,1992.64-69.
    [13]韩延明,杨凤,周安国.生长猪饲粮中添加微生物植酸酶或麦鼓对生产性能和植酸磷利用率的影响[J].四川农业大学学报,1995,13(3):327-331.
    [14]贺长青,黄生强,马海明.植酸酶对仔猪生长性能影响的研究[J].黑龙江畜牧兽医,2002,(5):14.
    [15]侯永清,梁敦素等.早期断奶仔猪日粮中添加不同种类酸化剂的效果[J].中国畜牧杂志,1996(6):8-10.
    [16]黄俊,韩铭海,陈小娥,等.新型微生物饲料添加剂的开发及应用效果研究 [J].饲料工业,2003,24(12):40-43.
    [17]霍启光.动物磷营养与磷源[M].北京:中国农业科学技术出版社,2002.
    [18]李德发.仔猪料中添加柠檬酸对营养物质消化率的影响[J].中国饲料1993(4):7-9.
    [19]李家携,于炎湖.黄曲霉毒素对产蛋鸡生产性能及健康影响的研究[J].华中农业大学学报,1991,10(3):290-294.
    [20]刘伯淳,于守洋主编.营养与食品卫生学[M].北京人民卫生出版社,1987:146-167.
    [21]刘忠琛.浅谈我国无公害猪肉生产存在的问题与对策[J].黑龙江畜牧兽医.2003(11):62-63
    [22]罗德元,李玉琼魔芋精粉对诱发小鼠肺癌的抑癌效果[J].中华肿瘤杂志,1992,14(1):48-50.
    [23]马玺,单安山.植酸酶研究进展及其在饲料工业中的应用[J].粮食与饲料工业,2001,(4):27-30.
    [24]茅彩萍,顾振纶魔芋的研究进展[J].中国野生植物资源,1998,17(4):15-18.
    [25]莫卫平,蒙义文,贾成禹,等葡甘聚糖凝胶及其衍生物的研究离子交换与吸附,1992,8(1):5-9.
    [26]潘红英,王德刚,郑卫兵.无公害肉猪生产的生物安全控制技术[J].畜牧兽医科技信息,2004(08):15-18.
    [27]荣富,陈克嶙,张春勇等.猪植酸酶营养及其环境意义[J].饲料工业,1999,20(12):27-291.
    [28]石宝明等.饲用酸化剂的作用与应用[J].饲料土业,1999,(1):3-5.
    [29]史凯来,王秀坤,刘伟等.PH值对植酸酶活性的影响及其在猪、鸡消化道中的分析[J].饲料研究,2000,(7):8-9.
    [30]唐宝英,朱晓慧,刘佳.新一代微生态制剂--酪酸菌的研究和开发前景[J].中国微生态学杂志,2000,12(5):297.
    [31]王春林,赵京扬.猪营养中植酸及植酸酶研究[J].中国饲料,2000,18:13-15.
    [32]王凤来,张曼夫,陈清明等.日粮磷和钙磷比例对小型猪(香猪)血清、肠、骨碱性磷酸酶及血清钙磷的影响[J].动物营养学报,,2001,13(1):36-J42.
    [33]王建华.霉变饲料的霉菌毒素与防霉去毒技术[J].动物科学与动物医学,1995,12(1):5-7.
    [34]王恬.无公害猪安全生产手册[M],北京:中国农业出版社.2008.111-119.
    [35]王学玲等.仔猪饲喂柠檬酸和乳酸的效果[J].饲料研究,1995(4):12-15.
    [36]项涛,何欣,丁丽敏等.鸡日粮中应用植酸酶的研究进展[J].饲料研究,1998,(12):13-15.
    [37]熊国平,王淑云,于继英.促进我国植酸酶发展的对策[J].中国饲料,2001,(19):6-7.
    [38]许万祥.有机酸味剂对保持仔猪健康和增进仔猪生长性能的生物学作用[J].饲料与畜牧,1997(1):25-27
    [39]杨桂苹.丁酸梭菌的生物学功能[J].中国微生态学杂志,1998,10(5):306-310.
    [40]袁森泉.霉菌饲料污染的处理方法[J].广东饲料,1999,3:23-25.
    [41]张雪平,陆俭,傅恩武.酪酸梭菌与双歧杆菌对肠道致病菌的体外生物拮抗作用[J].中国微生态学杂志,2001,13(5):260-262.
    [42]赵熙,冉陆,杨宝兰,等.丁酸梭菌活菌制剂对肠道菌群影响的研究[J].中国微生态杂志,1999,11(6):332-333,338.
    [43]钟道强.降低猪饲料中钙磷比例以提高植酸酶活性[J].饲料工业,2000,21(12):44.
    [44]周岩民等.不同酸化剂对饲料制粒性能及酸化效果的影响[J].粮食与饲料土业,1997(11):14-15.
    [45]朱靖环,杨永红,毛华明.植酸酶的研究与应用进展[J].微生物学杂志,2002,22(1):43-46.
    [46]朱梅梦,王军,陈建欣.植酸酶在饲料中的应用研究与展望[J].饲料广角,2002,(18):22-24.
    [47]朱仁俊,高士争,葛长荣等.植酸酶对仔猪饲料消化率的影响[J].饲料工业,2001,22(9):26-27.
    [48]朱晓慧,唐宝英,刘佳.酪酸菌对肠道有益菌的增殖作用和共生关系研究[J].中国微生态学杂志,2004,16(4):193-196.
    [49]庄志发,贺连智,冯紫慧.简述微生态活菌(酪酸菌)制剂的研究应用[J].山东食品发酵,1999(1):32-35.
    [50]Dell B L,et al.Complexation of phytate with proteins and cations in corn germ and oilseed meals.J.Agri.Food chem,1976,24(4):804-808.
    [51]UPACI-UB,Enzyme nomenclature recommendations,Supplement 1.Biochem Byophys Acta,1976,429:1.
    [52]Jongbloed A W et al.Effect of pelleting mixed feeds on phytase activity and the apparent absorbability of phosphorus and calcUm in pigs.Anim Feed Sci Tec.1990,28:233-242.
    [53]Kuroiwa T,IwanagaM,Kobari K.Preventive effect of ClostridUm butyricum M588 against the prolferation of ClostridUm difficile during antimicrobial therapy[J].J.Pn.A Inf.d.,1990(64):1425-1432.
    [54]Maddiah V T.Phytic acids studies.Proc Soc.Exp.Bio.Med,1964,115:391.
    [55]Mitchell.R.D,H.M.Edwards.Additive egects of phytase and 1,25-(OH)D3 on phosphorus utilization in broiler chicks.Poult.Sci,1996,228,75(supplt):76.
    [56]Nelson T S,et al.The availability of phytate phosphotus in soybean meal before and after treatment with a mold phytase.Poultry Science,1968174:813-820.
    [57]Pallauf J,G Rimbach,et al.Effect of phytase supplementation to phytase-rich diet based on wheat,barley and Soya on the bioavailability of dietary phosphorus,calcUm,magnesUm,znic and protein in piglets.J Agdbiol.Res,1994,47:39.
    [58]Peeler H T.Biological availability of nutrients in feeds:Availability of major mineralions.J Anim Sci,1972,35:695-712.
    [59]Pointllart A N,Fontaine and M.Thomasset.Phytate phosphorus utilization and intestinal phosphatases in pigs fed low phosphorus:wheat or corn diets.Nutr,Rep.Int.1984,29:473-483.
    [60]Power R.Phytase:The Limitations to its universal use and how biotechnology is responding.In:Lyons T P(ed.).Biotechnology In The Feed Industry.Alltech Technical Publication.Nicholasv81e(KY):Alltech Inc.1994.
    [61]Qian H,et al.Adverse Effects of Wide CalcUm:Phosphorus Ratios on Supplemental Phytase Efficacy for Weanling Pigs Fed Two Dietary Phosphorus Levels. J Anim.Sci, 1996,74:1288-1297.
    
    [62]Ravindran et al .Phytates:occurrence,bioavailability and implications in poultry nutrition. Poultry andAvain Biology Reviews,1995,6(2):125-143.
    [63]Shenermamn Von S E et al. In vitro and in vivo experiments on the hydrolysis of phytate.Activity of plant phytase.J Anim Physiol Anim Nutr, 1988, 60:64-75.
    [64]Singh M, A D Krorian. Inhibition of trypsin activity in vitro by ytate.JAgric.Food Chem, 1982,30:799.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700