抵当口服液治疗脑出血（急性期）的理论探讨与实验研究
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摘要
本文从理论和实验两个方面初步探讨了抵当口服液治疗脑出血(急性期)的机制。
理论研究部分回顾了古今治疗脑出血的研究概况，提出瘀阻脑络是脑出血的主要病理基础，瘀久化热，多与痰火相互搏结，而现痰热腑实证，故可以化瘀通腑法治之。并在此基础上探讨了化瘀通腑法的代表方抵当汤制成的抵当口服液治疗脑出血的理论依据。
实验研究从脑出血后脑水肿指数和脑细胞凋亡程度两个方面观察了抵当口服液的脑保护作用，并从自由基损伤，脑细胞耐缺氧能力等方面进一步探讨了抵当口服液的脑保护机制。结果显示抵当口服液对大鼠脑出血模型具有减轻脑水肿，降低丙二醛(MDA)，升高转铁蛋白(Tf)、转铁蛋白受体(Tf—R)、低氧诱导因子(HIF)，减轻出血后脑细胞凋亡的作用。说明本方治疗脑出血的机制是多组分、多靶点的，既有对疾病关键环节、关键靶点的直接作用，又有对疾病辅助环节、次要靶点的调节作用，通过对整体状态的综合调节，达到治疗脑出血的目的。
The main purpose of this paper is to study the treatment mechanism of Didang Oral Solution through reviewing literature and animal experiments
In the fist part ,we reviewed the general situation of studing cerebral hemorrhage with traditional Chinese medicine;according to that, we consider blood stasis outside brain vesseles is the main pathological base of cerebral hemorrhage.Blood stasis induces heat if not been elimilated .Most cerebral hemorrhage patients have phlegm and heat in their bodies and have the symptoms of astriction,fever, cough, gasp.So, removing blood stasis and purgation is a very effective therapy method for cerebral hemorrhage.
Didang Oral Solution has the effect of removing blood stasis and purgation,so we take it as the representative agent of removing blood stasis and purgation method.
In the second part,we study the influence of Didang Oral Solution to encephaledema, hypoxia inducible factor(HIF),transferrin(Tf), transferrin-receptor(Tf-R), brain cell apoptosis, nitrogen monoxide(NO), superoxide dismutase(SOD),MDA of cerebral hemorrhage rat models with immunohistochemistry .The results reveal that Didang Oral Solution could alleviate encephaledema and brain cell apoptosis,decrease MDA,increase SOD,NO, HIF ,Tf ,Tf-R in the rat brain.
理论研究部分回顾了古今治疗脑出血的研究概况，提出瘀阻脑络是脑出血的主要病理基础，瘀久化热，多与痰火相互搏结，而现痰热腑实证，故可以化瘀通腑法治之。并在此基础上探讨了化瘀通腑法的代表方抵当汤制成的抵当口服液治疗脑出血的理论依据。
实验研究从脑出血后脑水肿指数和脑细胞凋亡程度两个方面观察了抵当口服液的脑保护作用，并从自由基损伤，脑细胞耐缺氧能力等方面进一步探讨了抵当口服液的脑保护机制。结果显示抵当口服液对大鼠脑出血模型具有减轻脑水肿，降低丙二醛(MDA)，升高转铁蛋白(Tf)、转铁蛋白受体(Tf—R)、低氧诱导因子(HIF)，减轻出血后脑细胞凋亡的作用。说明本方治疗脑出血的机制是多组分、多靶点的，既有对疾病关键环节、关键靶点的直接作用，又有对疾病辅助环节、次要靶点的调节作用，通过对整体状态的综合调节，达到治疗脑出血的目的。
The main purpose of this paper is to study the treatment mechanism of Didang Oral Solution through reviewing literature and animal experiments
In the fist part ,we reviewed the general situation of studing cerebral hemorrhage with traditional Chinese medicine;according to that, we consider blood stasis outside brain vesseles is the main pathological base of cerebral hemorrhage.Blood stasis induces heat if not been elimilated .Most cerebral hemorrhage patients have phlegm and heat in their bodies and have the symptoms of astriction,fever, cough, gasp.So, removing blood stasis and purgation is a very effective therapy method for cerebral hemorrhage.
Didang Oral Solution has the effect of removing blood stasis and purgation,so we take it as the representative agent of removing blood stasis and purgation method.
In the second part,we study the influence of Didang Oral Solution to encephaledema, hypoxia inducible factor(HIF),transferrin(Tf), transferrin-receptor(Tf-R), brain cell apoptosis, nitrogen monoxide(NO), superoxide dismutase(SOD),MDA of cerebral hemorrhage rat models with immunohistochemistry .The results reveal that Didang Oral Solution could alleviate encephaledema and brain cell apoptosis,decrease MDA,increase SOD,NO, HIF ,Tf ,Tf-R in the rat brain.
引文
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