血管内皮生长因子C及细胞外基质与非小细胞肺癌
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摘要
目的：探讨非小细胞肺癌(NSCLC)中血管内皮生长因子C (VEGF-C)与细胞外基质(extra cellular matrix, ECM)中主要成份纤连蛋白(fibronectin, Fn)和Ⅳ型胶原蛋白(collagen IV, cIV)的表达及临床意义。方法：应用免疫组织化学SP法检测120例NSCLC癌组织以及这120例NSCLC癌旁组织、120例正常肺组织(癌旁组织取自相应肿瘤旁,距肿瘤病灶边缘大于2 cm,正常肺组织取自距肿瘤边缘5cm以上,两者的术后病理切片证实均为正常肺组织)中VEGF-C与ECM成分纤连蛋白(fibronectin, Fn)和Ⅳ型胶原蛋白(collagen IV, cIV)的表达,同时分析其与NSCLC患者临床病理特征之间的关系以及VEGF-C与纤连蛋白(fibronectin, Fn)和Ⅳ型胶原蛋白(collagen IV, cIV)表达的相关性。结果：NSCLC组织中VEGF-C. Fn和cIV的阳性表达率分别为95.8%、78.3%和50%,三者阳性表达率明显高于癌旁组织和正常肺组织(P<0.05)；Fn表达及VEGF-C强阳性表达与患者淋巴结转移有关；VEGF-C阳性表达与Fn表达呈正相关(r=0.757,P<0.001), VEGF-C阳性表达与cIV表达呈正相关(r=0.651,P<0.001)；VEGF-C阳性和Fn阳性与NSCLC患者预后正相关,(P<0.01)。结论：VEGF-C强阳性表达以及Fn表达与NSCLC患者淋巴结转移有关,二者在NSCLC转移中具有协同促进作用,影响预后,Fn可以作为新的靶向治疗的靶点。
Objective:To investigate the expressions and their clinical significance of vascular endothelial growth factor C(VEGF-C)and extra cellular matrix (ECM) components in non-small cell lung cancer (NSCLC).Methods:Expressions of VEGF-C and ECM components (fibronectin,FN and collagenⅣ,cIV) in 120 cases of NSCLC tissues and 120 cases of normal lung tissues and 120 cases of Carcinoma beside the organization were detected by immunohistological analysis.(cancer tissue adjacent from corresponding beside, is apart from the tumor lesion tumor edge greater than 2 cm, from normal lung tissue from the tumor edge 5cm above, both the postoperative pathologic open biopsy are normal lung tissue) Their relationship with clinical features of NSCLC and the correlation of exp ression of VEGF-C and Fn and cⅣwere analyzed.Results:The positive exp ression rates of VEGF-C,Fn,and cⅣwere95.8%,78.3%,and 50%in NSCLC tissues.The exp ressions of VEGF-C and Fn and cIV were significantly higher than those in normal lung tissues and Carcinoma beside the organization (P< 0.05).Fn expression and VEGF-C strong positive expression and patients lymph node metastasis are concerned.VEGF-C positive expression and positively correlated with Fn expression (r
0.757, P< 0.001), VEGF-C positive expression and positively correlated cIV expression (r= 0.651, P< 0.001),VEGF-C positive and Fn positive and the prognosis of patients with NSCLC positive correlation, (P<0.01). Conclusion:VEGF-C strong positive expression and Fn expression and NSCLC patients lymph node metastasis relevant, Both in NSCLC transfer has collaborative promoting effect, affect prognosis, Fn can be used as a new targeted therapy targets.
Objective:To investigate the expressions and their clinical significance of vascular endothelial growth factor C(VEGF-C)and extra cellular matrix (ECM) components in non-small cell lung cancer (NSCLC).Methods:Expressions of VEGF-C and ECM components (fibronectin,FN and collagenⅣ,cIV) in 120 cases of NSCLC tissues and 120 cases of normal lung tissues and 120 cases of Carcinoma beside the organization were detected by immunohistological analysis.(cancer tissue adjacent from corresponding beside, is apart from the tumor lesion tumor edge greater than 2 cm, from normal lung tissue from the tumor edge 5cm above, both the postoperative pathologic open biopsy are normal lung tissue) Their relationship with clinical features of NSCLC and the correlation of exp ression of VEGF-C and Fn and cⅣwere analyzed.Results:The positive exp ression rates of VEGF-C,Fn,and cⅣwere95.8%,78.3%,and 50%in NSCLC tissues.The exp ressions of VEGF-C and Fn and cIV were significantly higher than those in normal lung tissues and Carcinoma beside the organization (P< 0.05).Fn expression and VEGF-C strong positive expression and patients lymph node metastasis are concerned.VEGF-C positive expression and positively correlated with Fn expression (r
0.757, P< 0.001), VEGF-C positive expression and positively correlated cIV expression (r= 0.651, P< 0.001),VEGF-C positive and Fn positive and the prognosis of patients with NSCLC positive correlation, (P<0.01). Conclusion:VEGF-C strong positive expression and Fn expression and NSCLC patients lymph node metastasis relevant, Both in NSCLC transfer has collaborative promoting effect, affect prognosis, Fn can be used as a new targeted therapy targets.
引文
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