摘要
驼绒藜属植物为藜科半灌木旱生、超旱生植物,主要分布于北温带干旱、半干旱地区,目前该属植物全世界登记种有7个,中国有4个种和1个变种,其抗逆性强、营养丰富,具有良好的生态和经济价值。我国驼绒藜属植物花期较晚、结实率低、种子寿命较短、种群退化严重,为此我们从美国引进抗旱、抗寒、耐高温、生态幅宽、适应性强、开花结实早的北美驼绒藜进行引种驯化,为今后驼绒藜属植物品种选育及杂交育种奠定基础。本研究对在内蒙古引种的北美驼绒藜不同世代群体进行遗传变异规律的研究,包括生物学特性、形态学、细胞学、等位酶、分子遗传学等内容,主要
结果如下:
1.生物学特性研究结果表明北美驼绒藜4个世代群体间在物候期、开花动态、花粉活力、结实率、种子发芽率、活力指数及发芽指数等指标存在较大变异,而形态发育及叶片营养含量动态趋势变异较小;上述指标在世代间变异趋势为: BM0世代各指标测定值基本均小于其它三个世代,BM1世代次之,BM2和BM3世代最大且差异较小。
2.研究表明北美驼绒藜表型性状存在显著或极显著差异。其变异规律为:枝条长度、再生小枝数、叶长、叶宽、株幅的变异系数随世代的增加而减小;株高、叶腋雌花数、枝直径随世代的增加而增大;其它性状在4个世代间变异无规律。广义遗传力分析结果表明:株高、叶腋雌花数、叶宽、雄花穗长遗传力较高,表明受遗传因素影响较大;叶长、枝直径、株幅遗传力较低,表明受环境因素影响较大;表型性状的遗传、表型相关性分析表明,部分表型性状变异受环境和遗传因子共同影响,如枝条长度与着生雌花节数、枝条长度与再生小枝数、着生雌花节数与再生小枝数等性状;少数表型性状间的相关性主要受环境因素影响较大,如叶长和叶宽;其它大部分性状间的相关性主要受遗传因素影响较大;遗传增益分析结果表明:在5%的选择压力下,雄花穗长、再生小枝数、株高、叶宽遗传增益相对较高,表明该性状具有较高的遗传获得量。3.细胞学研究表明,北美驼绒藜世代间核型未发生明显变异,其核型均属1A型,
核型公式均为2n=2x=18=18m,染色体长度比和平均臂比变异系数较小,分别为7.66%和4.79%。
4.等位酶水平分析表明,北美驼绒藜4个世代群体多态位点百分率均为85.71%;北美驼绒藜群体的遗传多样性较大,其中BM1世代群体的遗传多样性最大(He =0.4229),BM3世代群体的遗传多样性最小(He =0.3714);北美驼绒藜的遗传变异主要来自世代群体内(97.2%);北美驼绒藜4个世代群体遗传一致度分析表明世代相隔越远,群体间遗传一致度越小,相隔越近,遗传一致度越大。
5.利用正交试验设计对北美驼绒藜ISSR-PCR反应的5因素(Taq酶、dNTP、引物、Mg2+和模板DNA)4水平进行试验,建立了适合北美驼绒藜的ISSR-PCR最佳反应体系,即20μl的反应体系中含有1×buffer,1.5U Taq酶,0.2mmol/L dNTP,0.5μmol/L引物,2.5 mmol/L Mg2+和10ng模板DNA。
6.北美驼绒藜ISSR-PCR分析共检测到167个位点,多态百分率为89.22%,各世代基因杂合度为0.2725~0.2306,shannon多样性指数为0.4441~0.3888,表明北美驼绒藜种内存在较丰富的遗传多态性;世代间多态性的大小规律为BM0到BM1增大,之后逐代减小;4个世代群体的遗传变异有83.49%分布在群体内,有16.51%分布于群体间;随着世代的增加,群体间遗传分化逐渐减小,世代群体间相隔越远,其遗传分化系数越大;聚类结果显示,BM2和BM3世代首先聚为一支,然后与BM1世代聚为一大支,最后与BM0世代再相聚。
Ceratoides belongs to Chenopodiaceae, which distributed extensively in Eurasia and mainly at arid and extra arid regions of the North Temperate Zone. This genera has 7 plant species all over the world, there are 4 plant species and 1 variation in China. This genera has great value of ecology and economy. Ceratoides in China owned the characteristics as later florescence, low setting percentage, shorter seed longevity and degeneration. On this account, we introduced Ceratoides lanata (Pursh) Powell of early florescence, high setting percentage. All these would provide academic base to breeding and crossbreed of new variety for the future. The paper chose different generations of C. lanata planting in China as the researching object, studied the regularity of genetic variation in biological characteristic, morphological, cytology, allozyme and DNA, revealed the characteristics and regularity of genetic variation after introduction, the main results were as follows:
1. 4 generations of C. lanata were varying in phonological phases, floral dynamic, pollen viability, seed setting rate, germination rate, vigor index and germination index, while varied little in growth and nutrition. Variation trends of indexes above in different generations were: BM0 generation basically was the smallest, and then BM1, BM2 and BM3 generations were the biggest and which two were close.
2. There was a larger variation of C. lanata phenotypic traits. Within generations, the variance coefficient of branch length, regenerated shoots, leaf length, leaf breadth, plant spread were all decreased while plant height, females flowers number in axil, branch diameter were all increased along with the increasing of generations. General potential analysis show that plant height, female flowers number in axil, leaf breadth, staminate flowers panicle length had higher genetic potential and were influenced mostly by genetic factor, while leaf length, branch diameter, plant spread had lower genetic potential and were influenced mostly by environmental factor; Correlation analysis about several phenotypic traits showed that between branch length and nodes number in female flowers, regenerated shoots, between nodes number in Female flowers and regenerated shoots were all influenced both by environmental and genetic factors, the relativity between leaf length and leaf breadth were influenced mostly by environmental factor, and relativities among other indexes were influenced mostly by genetic factor. Genetic gains analysis showed that under 5% choosing pressure, the genetic gains of staminate flowers panicle length, rege- nerated shoots, leaf breadth etc. were all higher.
3. The karyotype of 4 C. lanata generations were all belonged to 1A type which did not variance obviously, karyotype formula was 2n=2x=18=18m, and the coefficient of variation of chromosome length ratio and average arm ratio were 7.66% and 4.79%, respectively.
4. The results of allozyme showed that percent of polymorphic loci of 4 generations of C. lanata all was 85.71%. Different generations of C. lanata all had a larger genetic diversity, and in which BM1 was the largest while BM3 was the least. Genetic variation of C. lanata derived from 4 generations populations. The farther of generations leaved the smaller of genetic identity, and the closer of generations leaved the larger of genetic identity.
5. The orthogonal design was used to optimize ISSR amplification system of C. lanata in five factors (Taq DNA polymerase, dNTP, primer, Mg2+ and DNA templet) at four levels respectively. The best of reaction system of C. lanata was established, 20μl reaction system which contained 1×buffer, 1.5U Taq DNA polymerase, 0.2mmol/L dNTP, 0.5μmol/L primer, 2.5 mmol/L Mg2+ and 10ng template DNA.
6. Results of ISSR-PCR genetic variance of C. lanata showed that 167 loci were detected in 16 ISSR primers, polymorphic loci percent was 89.22%,the gene heterogeneity of 4 generations was 0.2725~0.2306,shannon index was0.4441~0.3888,it showed that there was polymorphism in C. lanata. There was abundant diversity in C. lanata population, from BM0 to BM1, the diversity was increased and after BM1, it was gradually decreased. There was 83.49% genetic variance within generations and 16.51% among generations. Along with the increasing of generation, genetic differentiation among generations was decreasing; and the farther of generations leaved the larger of genetic differentiation coefficient. The results of clustering showed that BM2 and BM3 was the first cluster which with BM1 was the second cluster, and then with BM0 to be the third cluster.
引文
1 王伯荪,彭少磷.植被生态学—群落与生态系统[M].北京:中国环境科学出版社,1997
2 陈家宽,杨继.植物进化生物学[M].武汉:武汉大学出版社.1994,48-93
3 Stebbines G.L. Artifical and natural hybrid in the Gramineae, tribe Horde IV. Two triploid hyrids of Agropyron and EIynus.Anler.J.Bot., 1950,37:338-392
4 Hamrick J.L, Godt M.J.W. Allozyme diversity in plant species. In: Brown H.D.,Clegg M.T., Kahler A. L. et al. plant population genetics, breeding and genetic resources. Sinauer Associates Inc, Sunderland, Massachusetts, USA, 1989, 43-63
5 葛颂,洪德元.遗传多样性及其检测方法,见:中国科学院遗传多样性委员会编,生物多样性研究的原理和方法[M].北京,中国科技出版社,1994:123-140
6 解新明,云锦凤.植物遗传多样性及其检测方法[J].中国草地,2000,22(6):51-59
7 徐炳声,洪德元.细胞分类学发展的现状[M].北京:科学出版社 1987:1-14
8 钱迎倩.生物多样性研究的原理与方法[M].北京:中国科学技术出版社,1994. 123-140
9 肖海峻.鹅观草种质资源遗传多样性研究[D].中国农业科学院学位论文,2007,5
10 根井正利,王家玉译.分子群体遗传学与进化论[M].北京:农业出版社,1983,204-244
11 刘荣宗.真核生物基因组 DNA 多态性[J].生物学通报,1997,32(4): 8-9
12 卫俊智,朱凤绥.植物染色体高分辨显带技术的发展[J].生物学通报,1988,23(10):5-8
13 Barbujani G, Pigliucei M. Geographical patterns of karyotype polymorphism in Italian populations of Ornithogalum montanum (Liliaceae)[J]. Heredity, 1989, 62: 67-75
14 Miyamoto J, Kurita S. C-band polymorphism in the karyotype of Paris tetraphyllaA. Gray (Lilia- ceae) [J]. Cytologia, 1990, 55(2): 301-313
15 沈浩,刘登义.遗传多样性概述[J].生物学杂志,2001,18(3):5-7
16 Vrijenhoek R C. Ecologieal differentiations among clones: the frozen niche model. in: Wbhrmann K, Loesehk V.(eds). Population Biology and Evolution. Springer Verlag, Berlin, 1984, 217-231
17 朱军.遗传学[M].中国农业出版社,2005:350
18 陈国庆,黄宏文,葛学军.濒危植物矮沙冬青的等位酶多样性及居群分化[J].武汉植物学研究,2005,23(2):131-137
19 蒙子宁,杨丽萍,吴丰等.斜带石斑鱼养殖群体遗传多样性的RAPD分析[J].热带海洋学报,2007,
26(2):44-48
20 魏昕,荣廷昭,潘光堂.墨白 964 群体 5 轮混合选择遗传变异的分子生物学研究[J].中国农业科学, 2006,39(2):237-245
21 李朝霞,李健,何玉英等.中国对虾人工选育快速生长群体不同世代间的 AFLP 分析[J]. 高技 术通讯,2006,16(4):435-440
22 Hmariek J L. Isoyzmes, analysis of genetic structure of Plants Populations. In SoltisD, Soltis P. (EDS) Isozymes in Plant biology. Washington: Dioscorides Press, 1989, 87-105
23 Barrett S C H, Eckert C G、Variation, evolution of mating systems in seed Plants. In: Kawano S ed. Biological Approaches, Evolutionary Trends in plants. London: Academic Press, 1990, 229-254
24 Loveless M. R, Hamrick J. L. Ecological determinant of genetic structure in plant populations[J]. Ann Rev.Eco.Syst, 1984, 15:65-95
25 Barrett S C H, Shore J S. Isozyme variation in colonizing Plants.In: Soltis D, Soltis P ed. Isozyme in Plant Biology. Washington: Dioscorides Press, 1989, 106-126
26 张学江.中国卧龙自然保护区不同海拔川滇高山栋群体的遗传变异[M].中国科学院研究生院, 2006:6
27 周纪纶,郑师章,杨持.高等教育出版社[M].1992:293-394
28 崔明昆.苔鲜植物的生态变异及其分类学中的生态学准则[M].云南师范大学学报,2001,21 (4):61-64
29 王欣,雷家军, 张丽娜等.渥丹百合种内居群形态多样性研究[J].沈阳农业大学学报,2007, 38(6): 796-800
30 O’Hanlon P C, Peakall R, Briese DT.A Review of new PCR-based genetic markers and their utility to weed ecology. Weed research, 2000, 40:239-254
31 Anderson E. Introgressive hybridization. Biological Reviews of the Cambridge Philosophical Society, 1953, 28, 280–307
32 Van Droogenbroeck B, Kyndt T, Romeijn-Peeters E. Gheysen G Evidence of natural hybridization and introgression between Vasconcellea species (Caricaceae) from southern Ecuador revealed by chloroplast, mitochondrial and nuclear DNA markers. Annals of Botany, 2006, 97, 793–805
33 Barluenga M, Stolting KN, Salzburger W. Meyer ASympatric speciation in Nicaraguan crater lake cichlid fish [J]. Nature, 2006,439, 719–723
34 Rieseberg LH, Raymond O, Rosenthal DM. Major ecological transitions in wild sunflowers facilitated by hybridization [J].Science, 2003,301, 1211–1216
35 张田,李作洲,刘亚令等.猕猴桃属植物的cpSSR遗传多样性及其同域分布物种的杂交渐渗与同塑[J].生物多样性 2007,15(1):1-22
36 Daniel L H, Andrew G C. Source of variation. in: Daniel L H,Andrew G C.(eds) Principles of Population Genetics. Sinauer Associates, Inc. Publishers. Sunderland, Massachusetts. 1997
37 An1Selleml, Nyoer J L, Bourgeois TLE. Comparison of genetic diversity of the invasive weed Rubus alceifolius Poir.(Rosaeeae) in its native range and in areas ofintroduction, using amplified fragment length Polymorphism(AFLP)markers. Molecular Ecology, 2000, 9:443-455
38 Kolbe JJ, Glor RE, Schettino LR. Genetic variation increases during biological invasion by a Cuban lizard. Nature, 2004, 431:177-181
39 Mccauley DE, Smith RA, Lisenby JD, Hsieh C. The hierarehieal sPatial Distribution of chloroplast DNA Polymorphism across the introdueed range of Silene vulgaris. Moleeular Ecology, 2003, 12:3227-3235
40 贾继增.分子标记种质资源鉴定和分子标记育种[J].中国农业科学,1999,29(4):1-10
41 尚 占 环 , 姚 爱 光 . 生 物 遗 传 多 样 性 研 究 方 法 及 其 保 护 措 施 [J]. 宁 夏 农 学 院 学报,2002,23(l):66-69
42 冯 夏 莲 , 何 承 忠 , 张 志 毅 等 . 植 物 遗 传 多 样 性 研 究 方 法 概 述 [J]. 西 南 林 学 院 学报,2006,(1):51-59
43 Priee S C. Estimates of Population different liation obtained from enzyme Polymorphisms and quan- titative characters. Heredity, 1984, 75:141-142
44 张吉宇,袁庆华,张文淑.我国牧草种质资源及其遗传多样性的研究进展[J].中国草地, 2003, 25(3): 59-65
45 Market C L and Moller F. MultiPle Forms of Enzymes, Tissue, Oniogenetie and Species Specific Pattern [J]. Proceedings of National Academic Science, 1959, 45:753-763
46 Staub J E, Serquen F C, Gupta M. Genetic markers map construction and their application in plant breeding[J]. Hort Science, 1996, 31(5):729-741
47 沈 法 富 , 刘 风 珍 , 于 元 杰 . 分 子 标 记 在 植 物 育 种 中 的 应 用 [J]. 山 东 农 业 大 学 学报,2003,28(1):83-91
48 Beekillann JS, Soller M. Restriction fragment length Polymorphisms in Plant genetic improvement. Oxford Surveys of Plant Molecular and Cell Biology, 1986, 3:196-250
49 Williams J G, Kubelik A R, Livak K J, et al. DNA Polymorphisms Amplified by Arbitrary Primers are Useful as Genetic markers. Nuel.Aeeds.Res. 1990, 18(22):6531-6535
50 Welsh J, Mclenland M. Fingerprinting Genome Using PCR with Arbitrary Primers[J]. Nuel.Aceds Res, 1990, 18(24):7213-7218
51 刘华,贾继增.指纹图谱在作物品种鉴定中的应用[J].作物品种资源.1997(2):45-48
52 Romero C, Pedrye A, Munoz V. Genetic Diversity of Different Apricot Geographical Groups Deter- mined by SSR Markers.Genome, 2003, 46(2):244-252
53 高翔,庞江喜,斐阿卫.分子标记技术在植物遗传多样性研究中的应用[J].河南农业大学学报2002,36(4):356-359
54 Zieticwiez E, Rafalskia, Labuda D. Genome fingerprinting by simple sequence repeats(SSR) anchored polymerase chain reaction amplification[J].Genomics,1994,20:176-183
55 Davila J A, Loaree Y, Ferrer E. Molecular characterization and genetic mapping of random amplified microsatellite polymorphism in barley[J]. Theoretical and Applied Genetics, 1999, 98:265-273
56 Areade A, Anselin F, Faivare R P, et al. Application of AFLP, RAPD and ISSR markers to genetic mapping of European and Japanses larch. Theoretical and Applied Genetics, 2000, 100:299-307
57 Casasoh M, Mattioni C, Cherubina, et al. A genetic linkage map of European chestnut (Castanea sativa Mill.)based on RAPD, ISSR and isozyne markers. Theoretical and Applied Genetics, 2001, 102:1190-1199
58 王建波.SSR 分子标记及其在植物遗传学研究中的应用[J].遗传,2002,24(5):613-616
59 张木清,洪艺殉,李奇伟等.中国斑茅种质资源分子多态性分析[J].植物资源与环境学报, 2004,13(l):l-6
60 Sankar A A, Moore G A. Evaluation of inter simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map[J]. Theor.App.l Genet., 2001, 102: 206-274
61 Moreno S, Martin J P, Ortiz M. Inter-simple sequence repeats PCR for characterization of closely related grapevine germplasm[J]. Euphytica, 1998, 101:117-125
62 Wolfe A D, Randle C P. Relationships within and among species of the holoparasitic genus Hyobanche (Orobanchaceae) inferred from ISSR banding patterns and nucleotide sequences [J]. Syst. Bot., 2001, 26:120-130
63 K.vijayan, P. K. Kar, A. Tikader, et al. Molecular evaluation of genetic variability in wild populations of mulberry (Morusserrata Roxb.) [J].Plant Breeding, 2004, 123: 568- 572
64 Li.C.F.Quiros. Sequencerelated amplified polymorphism (SRAP), a new marker system based a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet, 2001, 103:455-461
65 李爱青.红麻种质资源遗传多样性及其分子标记研究[D]. 湖南农业大学博士学位毕业论文, 2006,7:11, 54-55
66 Seott K D, Eggler P, Seaton G, et al. Analysis of SSRs derived from grape ESTs. Theore- tieal and Applied Genetics, 2000, 100:723-726
67 马啸,张新全,周永红等.高羊茅的分子标记应用进展[J].草业学报,2006,15(2):35-43
68 曾兵.鸭茅种质资源遗传多样性的分子标记及优异种质评价[D]. 四川农业大学博士学位毕业论文,2007:11
69 杜 春 芳 , 刘 惠 民 , 李 润 植 . 单 核 昔 酸 多 态 性 在 作 物 遗 传 及 改 良 中 的 应 用 [J]. 遗传,2003,25(6):735-739
70 徐柱 ,王照 兰 ,肖海俊 .中 国牧草 种质资源 研究利用 及牧草种 子生产 [J]. 中国草地,2000,1:73-76
71 徐胜,张新全.19 份野生扁穗牛鞭草种质农艺性状遗传变异的数量化研究[J].中国草地,2003, 25(4):15-20
72 李景欣,云锦凤.不同生境条件下冰草穗部性状的变异[J].草业科学,2004,21(12):73-77
73 逯晓萍,米福贵,郭世华等.高丹草(高粱×苏丹草)主要农艺性状的遗传参数研究[J].华北农学报.2004,19(3):22-25
74 何文利,陈丽,苏雅拉.紫花苜蓿不同品种(系)形态学性状变异的研究[J].内蒙古科技与经济, 2004, 21:46-47
75 Falcinelli M, Cenci CA, Negri V. An assessment of Dactylis glomerata L. ecotypes. I. Botanical characteristics. Rivista-di-Agronomia, 1983, 17(2):305-313
76 M Aasmo Finne, D A Rognli, I Schjelderup. Genetic variation in a norw egian germplasm collection of white clover [J]. Euphytica, 2000, 112:33-44
77 Araujo M.R.A. et al. Genetic variation and correlation of agronomic traits in tall fescue [J].Canadian Journal of Plant Science, 1983, 63:453-460
78 Nguyen, H.T, Sleper, D.A. Genetic variability of seed yield and reproductive characters in tall fescue [J]. Crop Science, 1983, 23:621-626
79 王洪刚,孔令让,李平路等.中间堰麦草与小麦杂交后代的细胞遗传学及性状特点的研究[J]. 作物学报, 1999, 25(3):373-381
80 孙振雷,刘海学,李景欣.赖草联会消失突变体的细胞遗传学研究[J]. 草业学报, 1994,3(1):44-47
81 冯霞,孙振元,刘建锋等.多年生黑麦草核型分析与组织培养再生植株染色体变异研究[J]. 林业科学研究,2005,18(3):321-324
82 魏秀华,周永红,杨瑞武等.鹅观草属三个物种及其居群间核型变异研究[J]. 草业学报, 2005, 14(2): 57-62
83 张谦,郑国.长期培养的红豆草愈伤组织及其分化芽中染色体变异的研究[J].兰州大学学报, 1996,32(4):110-117
84 阎贵兴.中国草地饲用植物染色体研究[M].呼和浩特:内蒙古人民出版社,2001
85 C. H. Park, N. S. Kim, P. D. Walton .The Giemsa C-Banded Karyotype of Canada Wildrye (Elymus canadensis) . Plant Breeding, 1990, 104(3):248–251
86 R. Issolah, H. Benhizia, N. Khalfallah Karvotype Varyotype within Some Natural Populations of Sulla (Hedysarum coronarium L., Fabaceae) in Algeria Genetic. Resources and Crop Evolution , 2006, 53(8):1653-1664
87 王中仁.植物等位酶分析[M].北京:科学出版社,1996: 1-73, 10-11,145-163
88 张春,周永红,于海清等.鹅观草属、披碱草属、猬草属和仲彬草属物种的酯酶同工酶分析[J]. 四川农业大学学报, 2006,24(2):131-134
89 洪锐民,王昱生,黄大明等.野古草种群克隆的遗传变异和遗传结构[J]. 生态学报,2004, 24(5): 908-913
90 王可青,葛颂,董鸣.根茎禾草沙鞭的等位酶变异及克隆多样性[J]. 植物学报,1999, 41(5):537-540
91 崔继哲,曲来叶,祖元刚.微生境下羊草两种生态型种群的遗传多样性及遗传分化—等位酶分析[J].生态学报, 2000,20(3):434-439
92 Francois Balfourier, Gilles Charmet, Catherine Ravel. Genetic different iation within and between natural population of perennial and annual ryegrass [J]. Heridity, 1998, 87
93 Carlos F Quiroz. Identification of alfalfa plants by enzyme electrophoresis [J]. CropSci, 1980, 20: 262-264
94 M R Morales Corts, M C Crespo Martinez. Variation of PGM and IDH isozymes for identification of alfalfa varieties [J]. Euphytica, 2000, 112:137-143
95 Yamashita M, Abe J, Shimamoto Y. Variation and differentiation of allelic frequencies of isozyme loci in Perennial ryegrass (Lolium perenne) [J]. Journal of Japanese Society of Grassland Science, 1993, 38(4):459-468
96 Balfourier F, Charmet G. Methodological study of genetic resources conservation of perennial ryeg- rass by pooling wild populations [J]. Genetics Selection Evolution, 1994, 26:203-218
97 Schmelzer G H, Renno J F. Genotypic variation in progeny of the agamic grass complex Pennisetum section Brevivalvula in West Africa [J]. Plant Systematics and Evolution. 1999, 215:1-4, 71-83
98 王洁,陈孝,倪丕冲等.应用 RAPD 技术研究不同种山羊草 叶绿体 DNA 的遗传变异[J].作物学报,1998, 24(5):520-528
99 张利,郑有良,魏育明等.仲彬草属物种的 RAPD 遗传变异分析[J]. 北京林业大学学报,2003, 25(3): 11-16
100 解新明,卢小良.利用 RAPD 标记分析狼尾草属牧草品种间的遗传关系[J].草业学报,2005, 14(2): 52-56
101 韩冰,王学敏等.驼绒藜属植物 种 质资 源 遗 传多 样 性 RAPD 分 析 [J].草 地学报, 2002,11(2):155-158
102 Weaver K R.DNA amplification fingerprinting and hybridization analysis of centipede grass[J]. Crop Sci., 1995, 35:881-885
103 Edwards K, Johnstone C, Cthom pson. A simple and rapid method for the preparationof plant gen- omic DNA for PCR analysis [J]. Nucllear Acids Res, 1991, 19:13-49
104 Caetano A, Callahan G I M, illiams P E et al. DNA amplification fingerprinting analysis of bermudagrass (Cynodon dactylon) genetic relationships between species and interspecific crosses [J] There Appl Genet, 1995, 9:228-235
105 万平,王苏玲,陈佩度等.利用RFLP分子标记确定导入小麦的鹅观草(R.kamoji)染色体的部分同源群归属[J]. 遗传学报, 2002,29(2):153-160
106 王秀娥,陈佩度,周波等.小麦-大赖草易位系的 RFLP 分析[J].遗传学报, 2001,28(12): 1142-1150
107 杭焱,金燕,卢宝荣.濒危植物华山新麦草(Psathyrostachy shuashanica)的遗传多样性及其保护[J]. 复旦学报,2004,43(2):260-266
108 Kidwell KK, Austin DF, Osborn TC. RFLP evaluation of nine Medicago accessions representing the original germ plasm sources for north American alfalfa cultivars. Crop Science, 1994, 34: 230 -236
109 Pupilli F, Molecular. cytological and morpho-agro-nomic characterization of hexaploid somatic hybrids of Medicago[J]. Theoretical and Applied Genetics, 1995, 90:347-355
110 Xu W W, Sleper D A. Phylogeny of tall fescue and related species using RFLPs[J]. Theore. Appl. Genet., 1994, 88:685-690
111 Perez V, Osusky M. Molecular and cytogenetic characterization of repetitive DNA sequences from Lolium and Festuca. Applications in the analysis of Festulolium hybrids [J]. Theore.Appl. Genet., 1992, 84: 145-154
112 魏臻武,郭旺珍,张天真等.苜蓿遗传多样性的 SSR 和 ISSR 分析[J].草业科学,2002,(增刊): 180-182
113 魏臻武.利用 SSR,ISSR 和 RAPD 技术构建苜蓿基因组 DNA 指纹图谱[J].草业学报, 2004, 13(3):62-67
114 Jones E S, Dupal M P, Klliker R, et al. Development and characterization of simple sequence repeat(SSR)markers for perennial ryegrass (Loliumpe-renneL.) [J]. Theoretical and Applied Gene- tics, 2001, 102:405-415
115 刘文献,李立会,刘伟华等.华山新麦草居群取样策略的 SSR 分析[J].麦类作物学报, 2006, 26(2):16-20
116 珊丹,赵萌莉,云锦凤等.小麦微卫星引物在克氏针茅和冰草中应用的初步研究[J].中国草地, 2005, 27(4):20-24
117 Mian, M. A. Rouf, Saha. Malay C et al. Use of tall fescue EST-SSR markers in phylogenetic analysis of coolseason forage grasses. Genome, Aug 2005, 48(4):637-647
118 George, Julie, Dobrowolski et al. Assessment of genetic diversity in cultivars ofwhite clover (Tri- folium repens L.) detected by SSR polymorphisms. Genome, Aug 2006, 49(8):919-930
119 张利,周永红,丁春邦等.应用 ISSR 标记研究仲彬草属植物的遗传变异[J].广西植物,2006, 26(4): 375-380
120 席嘉宾,郑玉忠,杨中艺.地毯草 ISSR 反应体系的建立与优化[J].中山大学学报, 2004, 43(3):80-84
121 Dangi R S, Lagu M D, Choudhary L B et al. Assessment of genetic diversity in Trigonella foenu- graecum and Trigonella caerulea using ISSR andRAPD markers [J]. BMC Plant Biol.2004, 4(1): 13-24
122 Hao G, Lee D H, Lee J S et al. A study of taxonomical relationships among species of Korean Allium sect.Sacculiferum (Alliaceae) and related species using inter-simple sequence repeat (ISSR) markers [J]. Bot. Bull. Acad. Sin. 2002, 43:63-68
123 Matlaga D, Karoly K. Long-term grazing effects on genetic variation in Idaho fescue [J]. Range Manage. 2004, 57:275-279
124 Liston A, Wilson B L, Robinson W A et al. The relative importance of sexual reproduction verus clonal spread in an aridland bunchgrass [J].Oecologia, 2003, 137:216-225
125 Esselman E J, Jianqiang L, Crawford D J, et al. Clonal diversity in the rare Calamagrostis porteri spp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers [J]. Mol. Ecol. 1999, 8:443-451
126 姚家玲,洪柳,张友德等.骆炳山龙须草生态型的划分及其遗传差异的 AFLP 分析[J].中国农业科学,2004,37(11):1699-1704
127 白史且,高荣,沈翼等.假俭草遗传多样性的 AFLP 指纹分析[J].高技术通讯,2002,10 :45-49
128 O′Neill. NR, Van Berkum. PB, Lin. JJ et al. Application of Amplified Fragment Length Polymor- phism(AFLP) for the genetic characterization of Colletotrichum pathogens of alfalfa (Medicago sativa). Phytopathology, 1997,(8)7:745-750
129 Bert P F, Sourdille P. A highlydensity molecular map for ryegrass (Lolium perenne) using AFLP markers [J]. Thero Appl Genet., 1999, 99(3-4):445-452
130 Rodan-Ruiz I, Dendauw J. AFLP markers reveal high polymorphic rates in ryegrass (Loliumspp) [J]. Molicu Breeding., 2000, 6(2):125-134
131 张力君.驼绒藜属等几种旱生植物的持水力和蒸腾作用研究[D].内蒙古农业大学博士学位论文, 2003
132 易津,王学敏,乌仁其木格等. 驼绒藜属植物生物学特性研究进展[J].草地学报,2003,11(2):88-89
133 Neilson J A. 1968.New and important additions to the flora of the southwestern Yukon Territory [J]. Canada.Can.Field Natur., 82:114-119
134 Woodmansee R G, Potter L D. 1971. Natural reproduction of winterfat (Eurotia lanata) [J].Range Manage, 24:24-30
135 Romo J T, Redmann R E, Kowalenko B L et al. Growth of winterfat fowling defoliation in Nor- thern Mixed Prairie of Saskatchewan [J].Range Manage. 1995,48, 240-245.
136 Budd A C, Campbell J B. Flowering sequence of a local flora [J]. Range Manage. 1959, 12: 127-132
137 李柱, 杨刚,付爱良,沙吾列,郑晓红.新疆驼绒藜属(藜科)一些新分类群[J].植物研究, 2008, 28 (2): 138-142
138 刘虎俊,王继和,李爱德等.干旱沙区不同种源的绵毛优若藜表现性比较[J].西北植物学报, 2005,25(10):2030-2034
139 贺俊英,王六英,易津等.驼绒藜属 3 种植物开花习性及雌花柱头微形态研究[J].草地学报 2003,11(2):121-124
140 刘海英.驼绒藜属植物生殖生物学特性的研究[D].内蒙古农业大学硕士学位论文,2004
141 刘锦.华北驼绒藜生殖特性与种子败育相关性研究[D].内蒙古农业大学,2007
142 王六英,杜利霞,贺俊英等.驼绒藜属 4 种植物花粉母细胞减数分裂的观察及花粉活力的测定[J]. 内蒙古师范大学学报,2003,32(4):391-396
143 李青丰,易津.驼绒藜属植物种子萌发检验标准及幼苗发育特性的研究[J].内蒙古草业, 1994,(1,2):47-49
144 王学敏,易津.驼绒藜属植物种子萌发条件及其生理特性的研究[J].草地学报,2003,11(2):95-102
145 Mohajery A, Rasti M. Germination temperature forBromu tomentellusBoiss and Eurtia ceratoides [J]. See Science and Technology. 1995, 23(1): 241-243
146 Hou-Jianqiang, Romo J T. Effects of chemical stimulators ogermination of winterfat (Ceratoides lanata (Pursh) J. T Howell)[J]. Seed Science and Technology, 1998, 26(1):9-16
147 库尔班.尼扎米丁.驼绒藜种子发芽、出苗及成株过程的研究[J].新疆农业大学学报,2006, 29(2): 54-57
148 易津,曹自成,乌仁其木格.几种不同贮藏条件对华北驼绒藜种子寿命和活力的影响[J].内蒙古农牧学院学报, 1994,15(1):18-22
149 赵书元.华北驼绒藜种子寿命及其采收贮藏[J].内蒙古草业,1986,(3):42-44
150 乌仁其木格,易津、乔枫.储藏条件对华北驼绒藜种子生理生化特性的影响[C].驼绒藜属牧草种子生物学特性的研究论文集,2002,12:53-56
151 孙祥,陈亚凡.华北驼绒藜根系的初步研究[J].中国草地,1994,(4):39-44
152 王六英,贺俊英,易津等. 华北驼绒藜胚胎发育研究[C].驼绒藜属牧草种子生物学特性的研究论文集,2002,41-44
153 Booth.T. Seedbed ecology of winterfat: cations in diaspore bracts and their effect on germination and early growth. Journal of Range Management 1989, 42(3):178-182
154 Kadyrova.R.B. Gavrilyuk.I.P. Savoskin.I.P. Zaitseva.L.N. Immun℃ hemi cal analysis of species of Astragalus L. in relation to the search for highprotein forms. Trudy Prikladnoi Botanike, Genetike Selektsii 1981, 70(2):129-132
155 Oshanina.N.P. Azimova.T. Changes in contents of nitrogencontaining compounds in assimilating ortans of some desert plants under conditions of soil moisture deficit and high temperature Fiziolo- tiya biokhimiya dikorastushchikh rastenii Uzbekistana. 1975, 140-151
156 易津,王学敏,谷安琳等.驼绒藜属牧草种子水分生理及幼苗耐旱性研究[J].草地学报, 2003, 11 (2):103-109
157 张力君.干旱胁迫下驼绒藜幼苗游离脯氨酸积累与细胞膜透性变化[D].内蒙古农牧学院硕士学位论文,1985
158 王洪春.植物抗性生理[J].植物生理学通讯,1981,(6):72-81
159 何玉惠,蒋志荣,王继和.两种驼绒藜属植物的抗旱生理研究[J].甘肃农业大学学报,2005,40(2):212-215
160 库尔班·尼扎米丁.驼绒藜属饲用植物的初步研究[D].新疆八一农学院硕士学位论文,1987
161 Harper K T, Buren R Van, Kitchen S G, et al. Invasion of alien annuals and ecological consequences in salt desert shrublands of western Utah [J]. General Technical Report Intermountain Research Station. USDA Forest Service, 1996, 338:58-65
162 Chambers J C, Norton B E. Effects of gazing and drought on population dynamics of salt desert shrub species on the desert experimental range, Utah [J]. Journal of Arid Environments, 1993, 24(3):261-175
163 Khasanov O K H, Tadzhiev S F, Kamalov S H K, et al. Features of growing fodder plants in the Amu Darya river Delta [J]. Problems of Desert Development, 1996, (1):44-51
164 Tursunay. Comprehensive evaluation of salt resistance of eight herbage species[J]. Grassland of China, 1995, (1):30-32
165 Tashninova L N. Salt tolerance of planted shrubs in thesalinization of soils in Kalmykia [J]. Pochvovedenie, 1991, (1):86-93
166 陈峰.不同草原类型华北驼绒藜生殖生态学研究[D].内蒙古农业大学硕士学位论文,2007,14-22
167 杨九艳.两种驼绒藜属植物的植物学和生物学特性的研究[D].内蒙古农业大学硕士学位论文, 1988,17-38
168 富象乾,曹自成.三种驼绒藜属植物营养器官的解剖结构[J].中国草地,1995,(2): 32-37
169 祝建,马德滋.旱生植物驼绒藜茎的异常次生结构及其发育[J].西北植物学报,1992,(2): 23-27
170 祝建,马德滋.驼绒藜属植物茎的异常次生加厚的研究[J].宁夏农学院学报,1986,(3):19-22
171 库尔班·尼扎米丁.驼绒藜属三个种的核型分析[J].植物分类学报,1986,24(4):466-468
172 杨久艳,富象乾.三种驼绒藜属植物的染色体核型分析[J].中国草地,1996,(1):67-71
173 乌仁其木格, 易津,李洪涛.驼绒藜属植物牧草种子同工酶多态性研究[C].驼绒藜属牧草种子生物学特性的研究论文集,2002,68-72
174 乌仁其木格, 易津,贮藏条件对华北驼绒藜种子同工酶的影响[C].驼绒藜属牧草种子生物学特性的研究论文集,2002,57-61
175 库尔班·尼扎米丁,易津,王学敏.华北驼绒藜引种试验[J].中国草地,2004,26(1):78-81
176 Slauson.W.L. Ward.R.T. Ecotypic in winterfat in relation to reclamation in oil shale lands. Reclsm- stion and Revegetation Research. 1982,1(4):349-357
177 潘伯荣.三种沙漠牧草的引种比较[J].干旱区研究,1993,(5):32-36
178 Sun Hao feng. Introduction of woody forag Ceratoides arborescensin central Gansu [J]. Acta Prata- culurae Sinica, 1998, 7(1):19-23
179 樊学英,张众,索亚林等.华北驼绒藜育苗关键技术研究[J].内蒙古草业,2007,19(3):22-26
180 库尔班·尼扎米丁.驼绒藜属植物旱作建植技术的研究[J].新疆八一农学院学报,1992, 15 (3) :82-84
181 Ackerman.T.L.Germination and survival of perennial plants pecies in the Mojave Desert. Labora- tory of Nuclesr Medicine and Radiation Biology, University of California, Los Angeles, California, USA. Southwestern Naturalist, 1979, 24(3): 399-408
182 Booth.D.T. Schuman G. E. Seedbed ecology of winterfat fruits vs. threshed seeds J Range Manage 1983,36(3):387-390
183 索亚林,史云威,兰云峰等.驼绒藜天然草场改良及扩繁技术研究[J].内蒙古草业 2003(2):3-4
184 Dickore W B. Zonation of flora and vegetation of the northern declivity of the Karakoram/Kunlun mountains(SWXinjiang China).Geojournal. 1991, 25(2-3): 265-284
185 张新全.草坪草育种学[M].北京:中国农业出版社,2004,29-30
186 Booth, D.T. Winterfat diaspore morphology[J]. Range Manage, 1988, 41:351-353
187 West N.E. Gasto J. Phenology of the aerial portions of shadescale and winterfat in Curlew Valley, Utah [J]. Range Manage, 1978, 31: 43-45
188 Clarke, S.E. Tisdale E.W. The chemical composition of native forage plants of southern Alberta and Saskatchewan in relation to grazing practices. Dominion of Canada, Dep. of Agric. Tech. Bull. 1945, 60
189 张志良,瞿伟菁.高等教育出版社,2004,3:225-226
190 南京农学院.土壤农化分析[M].北京:农业出版社,1980: 191-193
191 彭燕.野生鸭茅种质资源遗传多样性及优异种质筛选[D].四川农业大学博士学位论文,2006
192 刘学诗,刘建秀.中国东部假俭草种质资源多样性初步研究—物候期变异及其规律[J].园艺园林科学, 2004,20(5):180-186
193 李守勇,孙明高,李学宏等. 11 个黑杨无性系物候期变异分析[J].西北林学院学报,2003, 18(3):40-42.
194 侯伯鑫,林峰,余格非等.福建柏地理种源开花与结实变异规律的研究[J].植物遗传资源学报, 2005, 6(2):163-167
195 乌日娅,雍世鹏,包贵平.扁蓿豆生态生物学特性的比较研究[J].中国草地,1994,2:1-7
196 席嘉宾,陈平,刘长春等.中国地毯草野生种质资源的形态学特性和生物学特性的变异研究[J]. 四川草原,2004,12:15-19
197 谢海霞,陈冰,文启凯等.氮、磷、钾肥对“全球红葡萄”产量与品质的影响[J].北方园艺, 2005, 4(2):73-74
198 黄正来,武立权,韩立德.花期追施氮肥对菜用大豆生理指标及产量影响的研究[J].激光生物学报, 2005, l4 (3):193-196
199 中国科学院中国植物志编写组.中国植物志[M].北京:科学出版社, 2002, 39:89-90
200 李春喜,王志,王文林.生物统计学[M].科学出版社 1997, 13-15
201 陈禅友,胡金萍,刘伟等.豇豆品种品质性状及其遗传参数分析[J].江汉大学学报,2007, 35(3)64-68
202 于英,王秀全,张永刚等.北柴胡生长关键期主要性状的遗传参数及育种应用[J].吉林农业大学学报, 2006,28(4):415-418
203 宋锡章,张宝石.春玉米主要穗部性状配合力及遗传参数[J].中国农学通报, 2007, 23(6):245-249
204 王英,庄南生,王石华等.旱稻数量性状的遗传参数分析[J].热带作物学报,2005: 26(3):34-38
205 张振海,张俊杰,胡丽娟. 宁夏主推水稻品种品质性状遗传参数的分析[J].种子,2005: 24(10):69-70
206 马鸿翔,盛炳成,戴子林等. 草莓数量性状遗传变异研究[J]. 果树科学, 1995, 12:68-72
207 杨庆凯.大豆杂交材料主要农艺性状早代遗传变异的试验分析[J].遗传学报,1975, 2:225-230
208 周丰锁.大豆杂交后代主要农艺性状遗传与相关研究[J].作物学报,1981, 7(2):141-148
209 杨会勇.杨树无性系生长过程遗传变异及选择研究[J].南京林业大学,2005:27-30
210 季道藩.遗传学[M].北京:中国农业出版社,1980,87-92
211 宋云民,黄铨,黄永利.湿地松家系生长和材性遗传变异分析[J].林业科学研究,1995,8(6):671-676
212 马育华.植物育种的数量遗传学基础[M].南京:江苏科技出版社,1982
213 盛志廉,陈姚生.数量遗传学[M].科学出版社 1999
214 洪德元.中国和日本产竹叶子(亚种)(鸭跖草)核型的一致性[J].植物分类学报,1986,34(4):264-267
215 李贵全.细胞学研究基础[M].北京:中国林业出版社,2001:79-99
216 孙义凯,赵毓棠,董玉深等.东北地区小麦族 11 种植物的核型报道[J].植物分类学报,1992, 30(4):342-345
217 杨瑞武,周永红,郑有良等.11 个四倍体赖草属物种的核型研究[J].植物分类学报,2004, 42 (2):154-161
218 孙根楼,颜济,杨俊良.鹅观草属三个种的核型研究[J].云南植物研究,1992,14(2):164-168
219 王克平.披碱草的核型分析[J].遗传,1982.4(6):19-20
220 刘玉红.我国 11 种披碱草的核型研究[J].武汉植物学研究,1985,3(4):325-330
221 孙振雷,黄凤兰.赖草的染色体组型及非整倍体[J].哲里木畜牧学院学报,1998,8(2):10-13
222 孙根楼,颜济,杨俊良.仲彬草属和鹅观草属几个种的核型研究[J].植物分类学报,1993, 31 (6):560-564
223 孙彦,周禾,史德宽.新麦草有丝分裂及核型分析[J].草地学报,2000,8(3):193-197
224 冉雪琴,朱晓彤,朱邦长.贵州野豌豆属植物的核型研究[J].草业科学,1997,14(1):5-9
225 万黎明,张晓明.栽培沙打旺与野生沙打旺的核型分析[J].山西师大学报,1997,11 (3):52-55
226 张为民.四种紫花苜蓿的核型分析[J].山西农业大学学报,2005,26(1):74-76
227 贾纳提,维纳汗,李保军等.四种豆科牧草的染色体核型分析[J].草业科学,1996,13(4):11-13
228 黄华,郭水良.一枝黄花属(Solidago)三种欧洲入侵种的生态学研究概况[J].广西科学, 2004, 11(1):69-74
229 杨大翔.用 Adobe Photoshop 进行核型分析.2005,农业网络信息,3:45-46
230 蒋姗姗,梁英民,王作军.利用个人电脑系统及 photoshop 软件进行核型分析[J].第四军医大学学报,2000,l(7):860
231 李萍.青海云杉遗传多样性研究[D].甘肃农业大学博士学位毕业论文,2005,5:5-6
232 胡能书.万贤国.同工酶技术极其应用[M].湖南长沙:湖南科学技术出版社,1985
233 Weeden N.F. Lamb R.C. Genetics and Linkage Analysis of 19 Isozyme Loci in Apple [J]. Amer. Soc. Hor. Sci.1987, 112(5): 856-872
234 方宣钧,黄育民,陈启锋等.若干水稻品种(组合)的等位酶 RAPD 遗传分析[J].中国农业科学, 1999,32(2):1-8
235 顾万春.统计遗传学[M].北京:科学出版社,2004.156-185
236 蔡礼鸿.批把属的等位酶遗传多样性和种间关系及品种鉴定研究[J].中华农业大学 2000,5:25-27
237 韩冰.克氏针茅种群分化及不同退化系列生态变异的研究[D].内蒙古农业大学博士学位毕业论文, 2003,6:21-23
238 何忠效.电泳[M].北京:科学出版社,1999
239 Nei M. Genetic distance between populations [J]. American Naturalist, 1972, 106:283-292
240 内蒙古植物志编写组.内蒙古植物志第二卷[M].内蒙古人民出版社,1978,180
241 Hamrick J L, Godt M J W. Allozyme diversity on plant species [A]. Browm A HD, Clegg M T, Kahler et al. Population Genetic, Breeding, and Germplasm Resources[ C]. Sunderland Massachu- setts: Sinauer, 1989. 43-63
242 Hamrick J L, Godt M J W, Sherman-Broyles S L. Factors influencing levels of genetic diversity in woody plant species. New For, 1992, 6:95-124
243 Brown AHD, Moran G F. Isozymes and the genetic resources of forest trees. In:Conkte M T, eds. Proceedings of a Symposium on Isozymes of North American Forest Trees and Forest Insects.USA For Serv Gen Tech Rep,PSW-48, 1981,1-10
244 Gupta M,Chyi Y S,Romero-Severson J,et al. Amplification of DNA markefs from evolutionarily diverse genomes using single Primers of simple-sequence repeats[J]. Theor appl Genet, 1994, 89:998-1006
245 邱英雄,傅承新.明党参的濒危机制及其保护对策的初步研究[J].生物多样性,2000, 9(2):151-156
246 李巧明,许再富,何田华.濒危植物版纳青梅保护遗传学研究初报[J].植物学报,2002,44(2):246 -249
247 张志勇,李德锋.极度濒危植物五针白皮松保护遗传学研究[J].云南植物研究,2003, 25(5): 544-550
248 盖钧镒.试验统计方法[M].北京:中国农业出版社,2000:286-287
249 张萍,董玉芝,魏岩等.白梭梭ISSR-PCR实验反应体系的建立和优化[J]. 新疆农业大学学报, 2005, 28(4): 11-14
250 Yeh F C, Yang R C, Boyle T. POPGENE version 1.3.1. Microsoft windowbases freeware for population genetic analysis. http//:www.ualberta. ca/fyeh/: University of Alberta and the center for international forestry research, 1999
251 何正文,刘运生,陈立华等.正交设计直观分析优化 PCR 条件[J].湖南医科大学学报,1998, 23(4): 403-404
252 谢运海,夏德安,姜静等.利用正交设计优化水曲柳 ISSR-PCR 反应体系[J].分子植物育种, 2005, 3(3): 445-450.
253 林萍,张含国,谢运海.正交设计优化落叶松 ISSR-PCR 反应体系[J].生物技术,2005,15(5): 34-36
254 陈家宽,杨继.植物进化生物学[M].武汉:武汉大学出版社.1994
255 刘勋甲,郑用琏,刘纪麟.玉米轮回选择群体遗传多样性RAPD分子标记评估[J].中国农业科学, 1999, 32(3):14-20
256 吕宝忠、钟扬等译.分子进化与系统发育[M].高等教育出版社〔美〕Masatosh Sudhir kumar. 2002
257 刘塔斯,林丽美,龚力民等.分子标记中植物 DNA 提取方法的研究进展[J].中南药学2005:3(6): 370-373
258 陈大霞,李隆云,鲁成等.黄连 ISSR 反应条件优化的研究[J].植物研究,2007, 27(1): 77-81
259 金则新,李钧敏. 濒危植物夏蜡梅 ISSR 扩增条件的优化.植物研究[J]. 2007, 27(1):68-72
260 代红艳,张志宏,周传生等.山楂 ISSR 分析体系的建立和优化[J].果树学报, 2007,24(3): 313-318
261 姜静,杨传平,刘桂丰等.桦树 ISSR-PCR 反应体系的优化[J].生态学杂志,2003,22(3):91-93
262 汪结明,项艳,吴大强等.杨树 ISSR 反应体系的建立及正交设计优化[J].核农学报,2007,21 (5): 470-473.
263 曾 兵 , 张 新 全 , 范 彦 等 . 鸭 茅 种 质 资 源 遗 传 多 样 性 的 ISSR 研 究 遗 传 [J].2006, 28(9):1093-1100
264 Qian W. Genetic variation Within and among Populations of a wild rice Oryza granulate from China detected by RAPD and ISSR markers [J]. Theoretical and Applied Genetics, 2001, 102: 440 -449
265 卢萍,赵萌莉,韩国栋等.内蒙古小花棘豆遗传多样性的 ISSR 分析[J].西北植物学报,2007, 27 (6):1102-1107
266 张木清,洪艺殉,李奇伟等.中国斑茅种质资源分子多态性分析.植物资源与环境学报[J]. 2004, 13(l): l-6
267 Morgante M, Olivieri A M. PCR-amplified micro satellite markers in Plant genetics[J]. Plant Journal, 1993, 3:175-182
268 Depeiges A, Goubely C, Lenoir A, et al. Identification of the most represented repeated motifs in Arabidopsis thaliana micro satellite loci. Theoretical and Applied Genetics,1995,91:160-168
269 Oniwa K, Kijima A, Fujio Y. Relationship between genetic variability and quantitative traits in Japanese scallop, Patinopecten yessoensis. Tohoku J.Agr.i Res., 1994, 45:110-121
270 钱韦,葛颂.居群遗传结构研究中显性标记数据分析方法初探[J].遗传学报, 2001, 28(3):244-255
271 Thorpe J P. The molecular clock hypothesis: Biochemical evolution, genetic differentiation and systematic[J]. Annual Review of Ecology Systematics, 1982, 13:139-168
272 Wright S. The genetical structure of populations[J]. Annals of Eugenics, 1951, 15:323-334
273 Cox T.S., Mang Y.T., Gorman M.B. Relationship between coefficient of Parentage and genetic similarity indices in the soybean[J]. Crop Science, 1985, 25:529-532
274 蒋向辉,佘朝文,谷合勇,伍贤进,湖南 9 个地方观赏辣椒品种形态标记与 RAPD 标记的比较研究[J], 江苏农业科学, 2007, 6:119-122
275 严学兵.披碱草属遗传多样性研究[D].中国农业大学博士学位毕业论文,2005
276 Powell W, Morgante M, Andre C, et al. The compar ison of RFLP, AFLP and SSR markers for gemplasm analysis [J]. Molecular Breeding, 1998, 4: 173-177
277 Marsan PA, Castiglioni P, Fusari F, et al. Genetic diversity and its relationship to hybrid perfor- mance in maize as revealed by RFLP and AFLP markers[J]. Theor Appl Genet, 1998, 96: 219-227
278 Schut J W, Qi X, Stem P. Association between relationship measures based on AFLP markers, pedigree data and morphological traits in barley [J]. Theor Appl Genet, 1997, 95: 1161-1168
279 余汉勇,魏兴华,王一平等. 应用形态、等位酶和 SSR 标记研究水稻矮仔占衍生品种的遗传差异,中国水稻科学,2004,18(6):477-482
280 肖猛.濒危植物桃儿七的遗传多样性研究[D]. 四川大学博士学位毕业论文,2006,4
281 王芋华,粗枝云杉天然群体的遗传变异[D].中国科学院研究生院博士学位毕业论文,2006