广西莪术挥发油GC-MS指纹图谱的构建及其谱效关系的研究
|
摘要
本学位论文利用气相色谱.质谱联用仪(GC-MS)分析技术对广西道地产区的10批次广西莪术样品进行比较分析,采用国家药品监督管理局推荐的“中药色谱指纹图谱计算机相似性评价系统”进行模式分析及相似度计算,建立了广西莪术油GC-MS特征指纹图谱,得到广西莪术油GC-MS指纹图谱特征峰面积值；以不同浓度的广西莪术油分别处理鼻咽癌CEN-2细胞,噻唑蓝(MTT)法检测莪术油对CNE-2细胞的毒性和不同浓度的莪术油对CNE-2细胞生长增殖抑制作用；Hochest33342荧光染色法观察凋亡细胞的发生及形态学变化,流式细胞术检测其凋亡率,蛋白免疫印迹法检测核转录因子κB (NF-κB)蛋白的表达；将获得的10批次不同产地的广西莪术油特征峰面积值和莪术油对鼻咽癌CEN-2细胞的增殖抑制率进行灰关联度分析,对原始数据进行规格化,根据规格化值计算参考数列与比较数列的绝对差值、各特征峰与对肿瘤细胞的增殖抑制率的关联系数、各特征峰与对肿瘤细胞的增殖抑制率的关联度。结果如下：
(1)通过GC-MS检测了广西莪术油含有52种化学成分,鉴定了39种成分,主要为萜类及倍半萜衍生物；广西莪术油指纹图谱检测出20个共有特征峰,特征峰峰面积之和占总峰面积的93.0%以上,10批次样品具有较高的相似度(>0.90)；10批次广西莪术油的指纹图谱中各特征成分均得到了较好分离,具有特征性和唯一性。
(2)莪术油对鼻咽癌CNE-2细胞有较强的体外细胞毒性作用,明显的抑制鼻咽癌CNE-2细胞的生长,存在着剂量.时间-效应的关系；莪术油能诱导鼻咽癌CNE-2细胞NF-κB的凋亡,其分子机制之一可能是通过下调NF-κB的表达,从而达到抑制鼻咽癌细胞的增殖,促进凋亡,发挥抑制肿瘤生长的作用。
(3)莪术油中主要的抗肿瘤活性成分为莪术醇、莪术二酮、β-榄香烯、吉马酮、莪术酮,其中莪术醇与肿瘤细胞抑制作用具有高度关联(关联度>0.90),莪术二酮、β-榄香烯、吉马酮、莪术酮与肿瘤细胞抑制作用的关联度均在0.70以上。其它各特征峰代表的化学成分与其增殖抑制作用的关联度均在0.60-0.80之间；依据关联度的大小,各成分对抗肿瘤作用贡献的大小顺序为：莪术醇>莪术二酮>β-榄香烯>吉马酮>莪术酮>α-丁子香烯>δ-瑟林烯>β-石竹烯>β-榄香烯酮>a-蒎烯>α-石竹烯>桉油素>樟脑>2-壬醇>异龙脑莪术烯>δ-榄香烯>莰烯>D-柠檬烯>龙脑。广西莪术抗肿瘤作用是其“有效组分群”共同作用的结果。
本研究建立了广西莪术油GC-MS指纹图谱,确定了广西莪术油“有效组分群”指纹特征对药效贡献的大小,这为中药的“谱效关联”的指纹图谱的建立、中药的质量控制和药效评价标准的制定提供了新的思路和方法。
In this paper, gas chromatography-mass spectrometry (GC-MS) analysis was performed for10samples of genuine producing areas in guangxi from curcuma kwangsiensis. The TIL profiles were evaluated by "Computer Aided Similarity Evaluation System"(developed by Research Center for Modernization of Chinese Medicine, Central South University), the fingerprint of essential oil from Curcuma kwangsiensis was established, and the characteristic peak area of the GC-MS fingerprint was acquired. The nasopharyngeal carcinoma CNE-2cells were treated with curcumol oil at different concentration12.5,25.0,50.0and100.0μg/ml), negative control (Ethanol medium) and positive control (5-Fu). The toxicity of Curcuma oil to CNE-2cells and the effect of inhibiting proliferation were detected by MTT method, the apoptosis was observed and analyzed by hoechst33342flourescence staning and flow cytometry, the expression of NF-κB was detected with western blotting; The characteristic peak area from10sample of genuine producing areas and inhibiting proliferation rate to CNE-2cells were analyzed by grey relational methods, the original data was standardized. At the premise of the standardized data, we calculated the absolute difference of reference series and comparison series, the correlation coefficient and correlation of the characteristic peaks and inhibiting proliferation rate to tumor cells. The results as follows:
(1) Study on fingerprint of essential oil from Curcuma kwangsiensis:
We separated52kinds of components in Curcuma kwangsiensis essential oil and identified39different compounds by GC-MS, mainly terpenoids and sesquiterpene derivatives. The20feature peaks in fingerprints of Curcuma kwangsiensi were identified over93%of the total area of the peaks, the resemblance was higher among10samples(>90%). The characteristic peak area from10sample of genuine producing areas had good separation, characteristics and uniqueness.
(2) Study on pharmacodynamic of anti-tumor effect by Curcuma kwangsiensis essential oil:
The Curcuma oil had strong toxicity to CNE-2cells in vitro, and was capable of significantly inhibiting proliferation of CNE-2, and the effect of inhibiting proliferation was dose-effect relationship. The Curcuma oil induced the apoptosis of NF-κB in the CNE-2cells, the molecular mechanisms may be related to the down regulated of the NF-κB protein level, and thus inhibit the proliferation of nasopharyngeal carcinoma CNE-2cells and promote apoptosis, and inhibit the growth of tumor cells.
(3) Study on fingerprint's spectrum-effect from Curcuma kwangsiensis essential oil:
The main anti-tumor active ingredient in curcumol oil was curcumol, curdione, β-elemene, germacrone and curzerenone. The curcumol was highly correlated with the effect of inhibitiing proliferation to nasopharyngeal carcinoma cell (relational garde>0.90). The relational grade was more than0.70between the curdione, β-elemene, germacrone, curzerenone and the effect of inhibiting proliferation to nasopharyngeal carcinoma cell. The relational grade was between0.60-0.80between other characteristic peaks represent the chemical composition and the effect of inhibitiing proliferation to nasopharyngeal carcinoma cell. According to the relational grade, the sequence of contribution to inhibiting proliferation to nasopharyngeal carcinoma cell was Curcumol> Curdione>β-Elemene> Germacrone> Curzerenone>a-Caryophyllene>δ-Selinene>β-Caryophyllene>β-Elemenone> a-Pinene>a-Caryophyllene> Eucalyptol> Camphor>2-Nonanol> Isoborneol>Curzerene>δ-Elemene> Camphene> D-Limonene> Borneol. The anti-tumor effect of Curcuma kwangsiensis was the result of combined effect of "effective component group".
This study established the fingerprint of essential oil from Curcuma kwangsiensis, identified the effective component group represented by fingerprint contributed to pharmacodynamic, and provide a reference for establishing fingerprint of TCM's spectrum-effect relationship, TCM quality control and develop evaluation criteria of pharmacodynamics.
(1)通过GC-MS检测了广西莪术油含有52种化学成分,鉴定了39种成分,主要为萜类及倍半萜衍生物；广西莪术油指纹图谱检测出20个共有特征峰,特征峰峰面积之和占总峰面积的93.0%以上,10批次样品具有较高的相似度(>0.90)；10批次广西莪术油的指纹图谱中各特征成分均得到了较好分离,具有特征性和唯一性。
(2)莪术油对鼻咽癌CNE-2细胞有较强的体外细胞毒性作用,明显的抑制鼻咽癌CNE-2细胞的生长,存在着剂量.时间-效应的关系；莪术油能诱导鼻咽癌CNE-2细胞NF-κB的凋亡,其分子机制之一可能是通过下调NF-κB的表达,从而达到抑制鼻咽癌细胞的增殖,促进凋亡,发挥抑制肿瘤生长的作用。
(3)莪术油中主要的抗肿瘤活性成分为莪术醇、莪术二酮、β-榄香烯、吉马酮、莪术酮,其中莪术醇与肿瘤细胞抑制作用具有高度关联(关联度>0.90),莪术二酮、β-榄香烯、吉马酮、莪术酮与肿瘤细胞抑制作用的关联度均在0.70以上。其它各特征峰代表的化学成分与其增殖抑制作用的关联度均在0.60-0.80之间；依据关联度的大小,各成分对抗肿瘤作用贡献的大小顺序为：莪术醇>莪术二酮>β-榄香烯>吉马酮>莪术酮>α-丁子香烯>δ-瑟林烯>β-石竹烯>β-榄香烯酮>a-蒎烯>α-石竹烯>桉油素>樟脑>2-壬醇>异龙脑莪术烯>δ-榄香烯>莰烯>D-柠檬烯>龙脑。广西莪术抗肿瘤作用是其“有效组分群”共同作用的结果。
本研究建立了广西莪术油GC-MS指纹图谱,确定了广西莪术油“有效组分群”指纹特征对药效贡献的大小,这为中药的“谱效关联”的指纹图谱的建立、中药的质量控制和药效评价标准的制定提供了新的思路和方法。
In this paper, gas chromatography-mass spectrometry (GC-MS) analysis was performed for10samples of genuine producing areas in guangxi from curcuma kwangsiensis. The TIL profiles were evaluated by "Computer Aided Similarity Evaluation System"(developed by Research Center for Modernization of Chinese Medicine, Central South University), the fingerprint of essential oil from Curcuma kwangsiensis was established, and the characteristic peak area of the GC-MS fingerprint was acquired. The nasopharyngeal carcinoma CNE-2cells were treated with curcumol oil at different concentration12.5,25.0,50.0and100.0μg/ml), negative control (Ethanol medium) and positive control (5-Fu). The toxicity of Curcuma oil to CNE-2cells and the effect of inhibiting proliferation were detected by MTT method, the apoptosis was observed and analyzed by hoechst33342flourescence staning and flow cytometry, the expression of NF-κB was detected with western blotting; The characteristic peak area from10sample of genuine producing areas and inhibiting proliferation rate to CNE-2cells were analyzed by grey relational methods, the original data was standardized. At the premise of the standardized data, we calculated the absolute difference of reference series and comparison series, the correlation coefficient and correlation of the characteristic peaks and inhibiting proliferation rate to tumor cells. The results as follows:
(1) Study on fingerprint of essential oil from Curcuma kwangsiensis:
We separated52kinds of components in Curcuma kwangsiensis essential oil and identified39different compounds by GC-MS, mainly terpenoids and sesquiterpene derivatives. The20feature peaks in fingerprints of Curcuma kwangsiensi were identified over93%of the total area of the peaks, the resemblance was higher among10samples(>90%). The characteristic peak area from10sample of genuine producing areas had good separation, characteristics and uniqueness.
(2) Study on pharmacodynamic of anti-tumor effect by Curcuma kwangsiensis essential oil:
The Curcuma oil had strong toxicity to CNE-2cells in vitro, and was capable of significantly inhibiting proliferation of CNE-2, and the effect of inhibiting proliferation was dose-effect relationship. The Curcuma oil induced the apoptosis of NF-κB in the CNE-2cells, the molecular mechanisms may be related to the down regulated of the NF-κB protein level, and thus inhibit the proliferation of nasopharyngeal carcinoma CNE-2cells and promote apoptosis, and inhibit the growth of tumor cells.
(3) Study on fingerprint's spectrum-effect from Curcuma kwangsiensis essential oil:
The main anti-tumor active ingredient in curcumol oil was curcumol, curdione, β-elemene, germacrone and curzerenone. The curcumol was highly correlated with the effect of inhibitiing proliferation to nasopharyngeal carcinoma cell (relational garde>0.90). The relational grade was more than0.70between the curdione, β-elemene, germacrone, curzerenone and the effect of inhibiting proliferation to nasopharyngeal carcinoma cell. The relational grade was between0.60-0.80between other characteristic peaks represent the chemical composition and the effect of inhibitiing proliferation to nasopharyngeal carcinoma cell. According to the relational grade, the sequence of contribution to inhibiting proliferation to nasopharyngeal carcinoma cell was Curcumol> Curdione>β-Elemene> Germacrone> Curzerenone>a-Caryophyllene>δ-Selinene>β-Caryophyllene>β-Elemenone> a-Pinene>a-Caryophyllene> Eucalyptol> Camphor>2-Nonanol> Isoborneol>Curzerene>δ-Elemene> Camphene> D-Limonene> Borneol. The anti-tumor effect of Curcuma kwangsiensis was the result of combined effect of "effective component group".
This study established the fingerprint of essential oil from Curcuma kwangsiensis, identified the effective component group represented by fingerprint contributed to pharmacodynamic, and provide a reference for establishing fingerprint of TCM's spectrum-effect relationship, TCM quality control and develop evaluation criteria of pharmacodynamics.
引文
[1]ZHANGA JS, GUANA J, YANGA FQ, et al. Qualitative and quantitative analysis of four species of Curcuma rhizomes using twice development thin layer chromatography[J]. Journal of Pharmaceutical and Biomedical Analysis,2008, 48 (3):1024-1028.
[2]WILSON B, ABRAHAM G, MANJU VS, et al. Antimicrobial activity of Curcuma zedoaria and Curcuma malabarica tubers[J]. Journal of Ethnopharmacology,2005,99 (1):147-151
[3]国家药典委员会.中华人民共和国药典：一部[S].北京：中国医药科技出版社,2010.257-258.
[4]JIANHONG ZENG, PING DAI, LINGYUN REN, et al. Apoptosis-induced anti-tumor effect of Curcuma kwangsiensis polysaccharides against human nasopharyngeal carcinoma cells[J]. Carbohydrate Polymers,2012,89 (4): 1067-1072.
[5]YANGA FQ, LI ASP, CHENAY, etal. Identification and quantitation of eleven sesquiterpenes in three species of Curcuma rhizomes by pressurized liquid extraction and gas chromatography-mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis,2005,39 (3):552-558.
[6]DI-YA LU, YAN CAO, LING LI, et al. Comparative analysis of essential oils found in Rhizomes Curcumae and Radix Curcumae by gas chromatography-mass spectrometry[J]. Journal of Pharmaceutical Analysis,2011,1 (3):203-207
[7]CHUNHUI DENG, JIE JI, NING LI, et al. Fast determination of curcumol, curdione and germacrone in three species of Curcuma rhizomes by microwave-assisted extraction followed by headspace solid-phase microextraction and gas chromatography-mass spectrometry[J]. Journal of Chromatography A, 2006,1117 (2):115-120.
[8]王琰,王慕邹.莪术的质量研究[J].药学学报,2001,36(11)：849-853.
[9]周欣,梁光义.不同产地莪术挥发油的研究[J].华西药学杂志,2002,17(3)：201—202.
[10]冯磊,陶文沂,敖宗华,等.莪术挥发油的提取工艺和主要成分测定[J].无锡轻工业大学报,2003,22(1)：90-91.
[11]JUN LI, YUE LIU, JIAN-QIANG LI, et al. Chemical Constituents from the Rhizomes of Curcuma kwangsiensis[J]. Chinese Journal of Natural Medicines, 2011,9 (5):329-333.
[12]MOHAMED BOUAZIZ, THABET YANGUI, SAMI SAYADI, et al. Disinfectant properties of essential oils from Salvia officinalis L.cultivated in Tunisia[J]. Food and Chemical Toxicology,2009,47 (11):2755-2760.
[13]WENQIANG GUAN, SHUFEN LI, RUIXIANG YAN, et al. Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods[J].Food Chemistry,2007,101 (4): 1558-1564.
[14]陈伟,吴畏,黄开华,等.超临界流体技术在生物资源活性成分萃取中的应用及展望[J].上海农业学报,2009,25(1)：135—138
[15]王娟,刘汉清.不同提取方法对莪术油提取的影响[J].安徽医药,2007,11(2)：114-115.
[16]陈淑莲,游静,王国俊.超临界流体萃取分析蓬莪术挥发性成分[J].中草药,2000,31(2)：902-903.
[17]刘娜,余德顺,代明权,等.蓬莪术挥发油提取技术的研究及其化学成分分析[J].西南师范大学学报(自然科学版),2002,27(3)：430-432.
[18]游剑,于英伟,李青坡,等.莪术油微球的含量测定[J].中成药,2005,27(1)：25-28.
[19]王婷,李铁福,林绍强,等.莪术醇的分离鉴定及含量测定[J].中华中医药学刊,2008,26(5)：1018-1020.
[20]ZHU YE, ZHU XP. Determination of curcumol in Rhizoma Curcumae By GC[J]. Zhongguo Zhong Yao Za Zhi,2006,31 (5):389-390.
[21]朱亚尔,朱晓平.毛细管色相谱法测定莪术中莪术醇的含量[J].中国中药杂 志,2006,31(5)：389-390.
[22]魏福祥,邓小丽,陈晓,等.气相色谱法测定莪术挥发油中榄香烯含量[J].河北科技大学学报,2008,26(3)：219-221.
[23]项秀娣,陈素红,楼招欢.气相色谱法测定不同方法提取的莪术挥发油中β.榄香烯的含量[J].浙江中医药大学学报,2011,35(3)：397-399.
[24]乔丽,王静,袁子民RP-HPLC法测定β-榄香烯的含量及有关物质[J].中国现代药物应用,2010,4(5)：1—3.
[25]李成网,王唯红.高效液相色谱法测定莪术油中莪术二酮和牦牛儿酮的含量[J].中国实验方剂学杂志,2007,13(3)：12—14.
[26]孙艳涛,刘姝畅,张振秋.HPLC波长切换法测定莪术残油中莪术二酮莪术醇和吉马酮的含量[J].中华中医药学刊,2010,28(2)：391—392.
[27]边海疆,杜明荦,张振秋.高效液相色谱法测定莪术残油软膏剂中莪术醇、吉玛酮含量[J].中医学报,2010,25(6)：2230-2232.
[28]贾东明,姜义娜,薛胜霞.反相高效液相法测定莪术油凝胶剂中莪术醇和吉马酮含量[J].温州医学院学报,2007,37(5)：500-501.
[29]宋爱莉,殷玉琨.莪术油干预治疗肿瘤的研究及应用概况[J].山东中医药大学学报,2008,32(2)：172—174.
[30]赵华,汤为学.莪术油对人子宫内膜癌细胞株RL-95-2抑制作用的体外研究[J].实用妇产科杂志,2006,22(3)：158—160.
[31]宋利琼,张昌菊.莪术油联合干扰素对小鼠宫颈癌端粒酶活性和细胞凋亡的影响[J].上海中医药杂志,2006,40(7)：68-69
[32]宋爱莉,张敬涛,李静蔚,等.莪术油对乳腺癌癌前病变造模大鼠血液流变学及乳房微循环的影响[J].中华中医药学刊,2008,26(3)：458-460.
[33]WU WY, LUO YJ, CHEN JH, et al. Image cytometric DNA analysis of hepaticcarcinomas carried by mice treaed by curcuma aramatica oil [J]. Chin J IntegrTradit West Med Liver Dis,2009,9 (1):18-20.
[34]SHEN LC, WU WY, ZHANG WB, et al. Effect of curcuma aramatica oil on proliferating cell nuclear antgen of hepatoma in mice [J]. World Chin J Digest, 2007,7 (2):56-157.
[35]唐渊,李晓辉.莪术提取物对肝癌细胞系HepG2的抗癌作用及机制研究[J].中国药理学通报,2007,23(6)：790-794.
[36]JIAN ZHANG, TAO ZHANG, XINYU TI, et al. Curcumin promotes apoptosis in A549/DDP multidrug-resistant human lung adenocarcinoma cells through an miRNA signaling pathway[J].Biochemical and Biophysical Research Communications,2010,399 (1):1-6.
[37]盛辉,苑春莉,王医术.莪术油对肺癌SPC2A4细胞株的体外抑制作用[J].江西中医学院学报,2006,18(5)：51.52.
[38]徐立春,边可君,卜平,等.莪术醇修饰的肿瘤疫苗抗MFC效应的研究[J].现代肿瘤医学,2006,14(11)：1357.1359.
[39]湛学军,徐燕萍,谢大泽,等.发光法分析莪术油等中药对肝、胃癌细胞增殖活性抑制作用的实验研究[J].中国肿瘤临床,2007,34(1)：30-33.
[40]周 红,孙海,钟毅.莪术油对白血病细胞株的体外抑制作用[J].广州中医药大学学报,2004,21(2)：131.133.
[41]周红,钟毅,王沁,等.莪术油对白血病细胞体外净化作用的实验研究[J].华西药学杂志,2004,19(4)：256-260.
[42]李传伟,徐英萍,苗 芳,等.莪术油抗小鼠结肠癌效应的实验研究[J].泰山医学院学报,2005,26(2)：89-91.
[43]冯刚,黄涛,卢宏达,等.莪术油注射液对小鼠移植性S180肉瘤血管形成的抑制作用[J].肿瘤研究与临床,2005,17(4)：233-235.
[44]程剑华,刘伟胜,吴万垠,等.中药莪术油肝动脉灌注治疗原发性肝癌的临床研究[J].中医杂志,1999,40(1)：25-26.
[45]程剑华,常钢,吴万垠,等，莪术油和化疗药对照肝动脉灌注栓塞治疗原发性肝癌的临床研究[J]_中国中西医结合杂志,2001,21(3)：165—167.
[46]徐凯,罗海英,李柳宁,等.中药灌注栓塞为主综合治疗原发性肝癌的临床研究[J].中国中西医结合杂志,2005,25(4)：299-302.
[47]赵勤萍,张荣.浅谈中药有效部位研究对中药产业现代化的影响[J].山西中医,2006,22(3)：48-50.
[48]王智民.中药行业发展急需解决的关键问题分析[J1.中国中药杂志,2006, 31 (1)：81—84.
[49]万明珠,梁逸曾,赵晨曦,等.温莪术挥发性化学成分分析和指纹图谱研究[J].时珍国医国药,2009,20(10)：2428-2429.
[50]QIN HL, DENG AJ, DU GH, et al. Fingerprinting analysis of Rhizoma chuanxiong of commercial types using 1H nuclear magnetic resonance spectroscopy and high performance liquid chromatography method [J]. J Integr Plant Biol,2009,51 (6):537-44.
[51]林文津,徐榕青,张亚敏,等.莲子心提取物毛细管电泳指纹图谱研究[J].中国医药生物技术,2009,4(5)：345-348.
[52]刘和平,谢培山,田润涛.柴胡属药材皂苷高效薄层色谱指纹图谱的研究[J].中药新药与临床药理,2008,19(1)：38-42.
[53]张桂芝.肉桂的紫外光谱鉴定研究[J].时珍国医国药,2005,16(10)：951.953.
[54]牟新利,高鹏,付川.三维荧光中药指纹图谱研究[J].光谱实室,2010,27(1)： 180.183.
[55]韩明霞,周群,李全宏,等.不同产地葛根红外光谱的三级鉴定[J].光谱学与光谱分析,2009,7(2)：1851.1855.
[56]孙国祥,孙丽娜,毕开顺.基于整体化学键振动和价电子跃迁的光谱指纹定量法鉴定麻黄质量[J].中南药学,2010,8(1)：52-57.
[57]孙国祥,胡珊,金杰,等.中药X射线衍射指纹图谱专家系统网格(TCM-XFP-ESG)构建与应用[J].中南药学,2009,7(10)：766-769.
[58]杨红玲,吴鸣建,沈国鹏.射干的X射线衍射Fourier指纹图谱鉴定[J].时珍国医国药,2010,21(1)：181.182.
[59]刘红,邹童,张连华,等.益智挥发油的气相指纹图谱的研究[J].安徽农业科学,2008,36(36)：15961-15962.
[60]刘东辉,黄月纯,张子龙,等.薄荷饮片挥发油气相色谱指纹图谱研究[J].中国药业,2011,20(4)：28-30.
[61]孙玲,杨倩,王金虎,等.柴胡挥发油毛细管GC指纹图谱研究[J].中国药物警戒,2010,8(8)：462-464.
[62]黄天来,洪馨,工宁生.生姜挥发油成分气相色谱指纹图谱研究[J1.中药新药与临床药理,2003,14(2)：123.124.
[63]丁平,曾元儿,何智健,等.不同产地阳春砂挥发油气相色谱指纹图谱研究[J].中国药学杂志,2004,39(6)：418-420.
[64]ZENG JH, XU GB, CHEN X. Application of the chromatographic fingerprint for quality control of essential oil from GuangXi Curcuma kwangsiensis[J]. Med Chem Res,2009,18 (2):158-165.
[65]HU LF, LI SP, CAO H, et al. GC-MS fingerprint of Pogostemon cablin in China[J]. Journal of Pharmaceutical and Biomedical Analysis,2006,42 (2): 200-206.
[66]魏刚,林双峰,方永奇.气相色谱-质谱联用法分析石菖蒲水煎液主要化学成分[J].广州中医药大学学报,2005,22(2)：147—149.
[67]周欣,李章万,王道平,等GC-MS法建立温莪术挥发油指纹图谱研究[J].中国中药杂志,2004,29(12)：1138—1141.
[68]周婧,都晓伟,马宏跃,等.西洋参药材高效液相色谱法指纹图谱的研究[J].时珍国医国药,2006,17(12)：2381-2383.
[69]ZHANG TT, WU Y, HANG TJ. Study on HPLC fingerprint of flavonoids from Houttuynia cordataby comparing with fingerprint reference [J]. Zhong Yao Cai, 2009,32 (5):687-90.
[70]陶敬奇,闻莹莹,王 辉,等.五指毛桃指纹图谱的高效液相色谱-质谱联用研究[J].华南师范大学学报(自然科学版),2011,3(3)：77-79.
[71]张敏,王义明,罗国安.日本救心丹多维指纹图谱研究[J].药学学报,2006,41(12)：1161.1165.
[72]兰作平,曲中堂.试论中药指纹图谱的研究现状及其研究过程的标准化[J].西部药学,2006,3(2)：71-72.
[73]孙国祥,宋文景.中药的毛细管电泳指纹图谱的研究方法[J].中南药学,2008,6(6)：752-754.
[74]谷巍,巢建国,刘训红,等.安徽道地药材牡丹皮高效毛细管电泳指纹图谱研究[J].中国实验方剂学杂志,2011,17(7)：58—60.
[75]YISUN, TAO GUO, YIN SUI, et al. Fingerprint analysis of Flos Carthami by capillary electrophoresis[J]. Journal of Chromatography B,2003,792 (2): 147-152.
[76]张丽艳,罗君,李健,等.吴茱萸药材薄层色谱指纹图谱研究[J].中国实验方剂学杂志,2011,17(12)：72-74.
[77]冯毅凡,周漩,郭晓玲.向量夹角法评价不同产地与商品来源的高良姜挥发油指纹图谱相似度[J].中药材,2006,29(1)：10-13.
[78]王龙星,梁鑫淼.一种评价中药色谱指纹谱相似性的新方法：向量夹角法[J].药学学报,2002,37(9)：713-717.
[79]王成哲,卢佩,沈鸿章.基于模糊聚类的中药对照指纹图谱研究[J].天津科技大学学报,2007,22(2)：62-65.
[80]袁鹏,袁敏,胡跃明.模糊聚类分析法在郁金裂解色谱测定指纹图谱中的应用研究[J].计算机与应用化学,2005,22(3)：201—205.
[81]田兰,毕开顺,赵少春.白术的化学模式识别[J].中国中药杂志,2003,28(2)：52-55.
[82]蒋建平.色谱指纹图谱识别的人工神经网络方法对比研究[J].广东化工,2011,38(2)：58-59.
[83]汤丹,许毅,吴秋云.广藿香指纹图谱解析的人工神经网络方法研究[J].中药材,2004,27(7)：534-536.
[84]韦记青,孔德鑫,唐辉,等.基于主成分分析和聚类分析的野生战骨红外指纹图谱比较研究[J].广西植物,2011,31(4)：559-563.
[85]李晓红,熊志立,虞明阳,等.基于主成分分析的骨碎补药材乙醇和环己烷提取物的高效液相色谱指纹图谱及指标成分的定量分析[J].色谱,2009,27(4)：453-457.
[86]郝燕,董鸿晔,姜楠,等.基于主成分分析的中药色谱指纹图谱多维多息特征数据挖掘方法研究[J].中南药学,2007,5(3)：267-272.
[87]高华荣,孙相民.中药指纹图谱在现代中药制剂中的应用[J].中国实用医药,2009,4(14)：240-241.
[88]朱善岚,黄品芳.HPLC法测定莪术不同炮制品中姜黄素含量[J].海峡药学,2007,19(3)：52—53.
[89]罗春兰,吴爱琴.不同品种莪术挥发油成分GC-MS分析[J].广东药学,2005,15(2)：10.11.
[90]王智民.中药行业发展急需解决的关键问题分析[J].中国中药杂志,2006,31(1)：81.84.
[91]YI JL, GAO L, HUANG XD, et al. Nasopharyngeal carcinoma treated by radical radiotherapy alone:ten year experience in a single institute [J]. Int J Radiat Oncol Biol Phys,2006,65 (1):161-168.
[92]CHENG SH, TSAI SY, YEN KL, et al. Prognostic significance of parapharyngeal space venous plexus and marrow involvement:potential landmarks of dissemination for stage Ⅰ-Ⅲ nasopharyngeal carcinoma [J]. Int J Radiat Biol Phys,2005,61 (2):456-465.
[93]HARlOZINSKA A. Progress in molecular mechanisms of tumor metastasis and angiogenesis[J].Anticancer Res,2005,25 (5):3327-3333.
[94]DIVYA CS, PILLAI MR. Antitumor action of curcumin in human papillomavirus associated cells involves downregulation of viral oncogenes, prevention of NFκB and AP-1 translocation, and modulation of apoptosis[J]. Molecular carcinogenesis,2006,45 (5):320-332.
[95]DEISY HH, GARCIA OP, JORGE L, et al. The contribution of fruits and vegetables to dietary intake of polyphenols and antioxidant capacity in a Mexican rural diet:Importance of fruit and vegetable variety [J]. Food Research International,2011,44 (5):1182-1189.
[96]KATARZYNA P, IRENA BB, MARIOLA M, et al. Changes in male reproductive system and mineral metabolism induced by soy isoflavones administered to rats from prenatal life until sexual maturity [J]. Nutrition,2011, 27 (3):372-379.
[97]PATRICIA ALSP, JOAO ANL, VICTOR APF.Antioxidant protection of low density lipoprotein by procyanidins:structure/activity relationships Original Research Article[J]. Biochemical Pharmacology,2003,66(6):947-954..
[98]PHILIPP SAIKO, AKOS SZAKMARY, WALTER JAEGER, et al. Resveratrol and its analogs:Defense against cancer, coronary disease and neurodegenerative maladies or just a fad? Review Article[J]. Mutation Research/Reviews in Mutation Research,2008,658 (1):68-94.
[99]XU CHEN, JIANHONG ZENG. Investigation on Influential Factors of Volatile Oil and Main Constituent Content from Curcuma kzoangsiensis S. G. Lee C. F. Liang. In Guangxi Producing Areas[J]. Modern Applied Science,2008,2 (1): 53-57.
[100]SEN R, BALTIMORE D. Mufiple nuclear factors interact with the im-munoglobulinenhancersequencees[J]. Cell,2006,46 (5):705-716.
[101]LEE DK, KANG JE, PARK HJ, et al. FBI-1 enhances transcription of the nuclear factor-kappaB (NF-kappaB)-responsive E-selectin gene by nuclear localization of the p65 subunitofNF-kappaB[J]. JBiolChem,2005,280 (30): 27783-22791.
[102]MATSUOS, YAMAZAKIS, TAKESHIGE K, eta.l Crucial roles of binding sites for NF-kappaB and C/EBPs in IkappaB-zeta-mediated transcriptional activation[J]. Biochem J,2007,405 (3):605-609.
[103]KARIN M, CAO Y, GRETEN FR, et al. NF-kappaB in cancer:from innocent bystander to major culprit [J].Nat Rev Cancer,2002,12 (2):301-310.
[104]KIM HJ, HAWKE N, BALDWIN AS. NF-kappa B and IKK as therapeutic targets in cancer [J]. Cell Death Differ,2006,13 (2):738-747.
[105]DOLCET X, LLOBET D, PALLARES J, et al. NF-kB in development and progression of human cancer [J]. Virchows Arch,2005,446 (5):475-482.
[106]SALVATORE C, CAMARDA G, MGGSI CA, et al. NF-kappa B activation contributes to anthracycline resistance pathway in human ovarian carcinoma cell line A2780[J]. Int J Oncol,2005,27 (3):799-806.
[107]SIEBENLISTU, BROWN K, CLAUDIO E. Control of lymphocyte developmentby nuclear factor-kappaB [J]. Nat Rev Immunol,2005,5 (1): 435-445.
[108]BHAKAR AL, TANNIS LL, ZEINDLER C, et al. Constitutive NF-κB activity is required for central neuron survival [J]. Neuroscience,2002,22 (9): 8466-8475.
[109]XIAO-LONG HOU, KYOKO TAKAHASHI, KEN TANAKA, et al. Curcuma drugs and curcumin regulate the expression and function of P-gp in Caco-2 cells in completely opposite ways[J]. International Journal of Pharmaceutics,2008, 358 (1):224-229.
[110]DOLCET X, LLOBET D, PALL ARES J, et al. NF-kB in development and progression of human cancer [J]. Virchows Arch,2005,446 (5):475-482.
[111]IRBACH A, GOLD P, BINDER BR, et al. Signalingmolecules of the NF-kappa B pathway shuttle constitutively between cytoplasm and nucleus [J]. J Biol Chem,2009,277 (13):10842-10851.
[112]JASON HTW, VIVIAN WYL, KAZUO UYH, et al. A small molecule inhibitor of NF-κB, dehydroxymethylepoxyquinomicin (DHMEQ), suppresses growth and invasion of nasopharyngeal carcinoma (NPC) cells Original Research Article[J]. Cancer Letters,2010,287 (1):23-32.
[113]PEERARAT THAINA, PATTREEYA TUNGCHAROEN, MALINEE WONGNAWA, et al. Uterine relaxant effects of Curcuma aeruginosa Roxb.rhizome extracts[J]. Journal of Ethnopharmacology,2009,121 (3): 433-443.
[114]CHEN XU, ZHONG HAI-YAN, ZENG JIAN-HONG, et al. The pharmacological effect of polysaccharides from Curcuma Kwangsiensis on the oxidative status and expression of VCAM-1mRNA of thoracic aorta endothelial cell in high-fat-diet rats [J].Carbohydrate Polymers,2008,74 (3):445-450.
[115]胡延维,韩蓉.莪术油注射液的安全性试验研究[J].中国医院药学杂志,2006,26(7)：904-905.
[116]贾晓斌,陈彦,李霞,等.中药复方物质基础研究新思路和方法[J].中华中医药杂志,2008,23(5)：420-422.
[117]贺福元,邓凯文,石继连,等.中药有效成分群组方技术研究若干基础瓶 颈问题及数理特征化思路的提出[J].中药材,2009,32(1)：1.4.
[118]贺福元,邓凯文,邹欢,等.中药复方谱动学与谱效动力学差异性的研究[J].中国中医杂志,2011,36(2)：136—138.
[119]任德权.中药现代化德几个问题[J].中成药,2003,25(1)：1.3.
[120]罗国安,王义明,曹进,等.建立我国现代中药质量标准体系的研究[J].世界科学技术,2002,4(4)：5—11.
[121]李戎, 闫智勇,李文军,等.创建中药谱效关系学[J].中医教育,2002,(21)2：62-64.
[122]宁黎丽,毕开顺,王瑞,等.吴茱萸汤药效物质基础的方法学研究[J].药学学报,2000,35(2)：131.134.
[123]周立东.建议在天然药物研究中建立“定量组效关系”(QCAR)概念[J].世界科学技术,1999,(1)2：33—34.
[124]杨东风,梁宗锁.中药指纹图谱研究进展[J].中国药房,2007,18(6)：467-470.
[125]刘荣霞,叶敏,果德安.中药质量控制研究的思路与方法[J].中国天然药,2006,4(5)：332-337.
[126]王微.黄芩指纹图谱及谱效关系研究[M].北京：北京中医药大学,2009.43-61.
[127]李洁,孙静.实现中药质量控制突破的思考[J].中华中医药学刊,2007,25(8)：1739.1740
[128]崔红华,邱夏.中药质量标准现代化研究发展趋势[J].中国中医药信息杂志,2007,14(6)：6-7
[129]李萍,刘耀.中药指纹图谱的研究现状和发展趋势[J].中国药业,2009,18(8)：19-21.
[130]李戎,闫智勇,李文军,等.“谱效关系”研究是中药质量与药效标准规范的关键环节[J].医药简讯,2002,10(6)：18—20.
[131]LII SHENG-QING, LIU YUN-SHENG, YANG HUI, et al. Observation on gene augmentation of cytosine deaminase to neural stem cells and its expression in vitro [J]. Chiuese Journal of Clinical Rehabilitation,2005,9 (1):213-215.
[132]于秀琳,任雪松.多元统计分析[M].北京：中国统计出版社,1999.62.
[133]许良, 毕开顺.多元线性回归分析法在蒙药森登汤谱效关系解析中的应用[J].计算机与应用化学,2008,25(10)：1189.1192.
[134]YANG DE-GANG, CHEN GUO, WANG HUI, et al. Learning Vector Quantization Neural Network Method for Network Intrusion Detection[J]. Wuhan University Journal of Naturnal Sciences,2007,23 (1):147-150.
[135]邓聚龙.灰色系统基本方法[M].武汉：华中科技大学出版社,2005.23—30.
[136]许虹,黄丹,郑元.模糊数学在茶多酚药理学研究中的应用[J].数理医药学杂志,2002,15(1)：87-89.
[137]KONG ZHENG-MIN, ZHONG LIANG, ZHU GUANG-XI, et al. Differential multiuser detection using a novel genetic algorithm for ultra-wideband systems in lognormal fading channel [J]. Comput & Electron,2011,12(9):754-765.
[138]杨如乃,胡志忠,卢健.生物嗅觉神经系统模型的模拟与分析[J].生物医学工程研究,2007,25(3)：131.136.
[139]YANG XIN-LING, FU JUN, LUO ZHENG-GUO. Tea classification based on artificial using bionic olfactory neural networks [C]. Proceedings of Third International Symposium on Neural Networks,2006,397 (3):343-348.
[140]马丽,杨琳,金印彬,等.细胞外低钾对心室肌组织钠通道电导影响的计算机仿真研究[J].生物医学工程学杂志,2009,26(1)：1—3.
[141]张锦,李司铎,王如龙,等.KⅢ模型动力学特性研究[J].湖南大学学报,2008,35(3)：76-79.
[142]张强,张霜红,钱建明,等.用灰关联聚类法评价中国少数民族人口素质[J].现代预防医学,2003,30(5)：43-45.
[143]赵筱萍,范骁辉,余杰,等.一类基于组效关系神经网络模型的中药药效预测方法[J].中国中药杂志,2004,29(11)：1082-1084.
[144]刘雪松,施朝晟,程翼宇,等.基于自组织映射神经网络的中药注射剂质量快速鉴别方法[J]_分析化学,2007,35(10)：1483.1486.
[145]范骁辉,程翼宇.中药复杂组效关系的变结构神经网络辨识方法[J].高等学校化学学报,2004,25(11)：2004.2009.
[146]RUDOLPH MERWE, TODD KL, ZHENGDONG LU, et al.Fast neural network surrogates for very high dimensional physics-based models in computational oceanography Original Research Article[J].Neural Networks, 2007,20 (4):462-478.
[147]周立艳,梁生旺,王淑美,等.牡丹皮高效液相色谱“药效指纹图谱”研究[J].时珍国医国药,2008,19(6)：1337—1339.
[148]刘荣霞,叶敏,果德安.中药质量控制研究的思路与方法[J].中国天然药,2006,4(5)：332-337.
[149]吴悦涛.指纹图谱在制定中药质量标准研究中出现的问题与对策[J].黑龙江医药,2007,20(1)：64-65.
[150]薛飞群.中药复方TF-103抗球虫活性与指纹图谱组效关系研究[J].中国兽药杂志,2006,40(3)：1—5.
[151]钱俊,尹莲.加味四妙丸有效部位群HPLC指纹图谱归属分析及谱效关系研究[J].世界科学技术,2007,9(1)：40-43.
[152]刘荣华,余伯阳,陈兰英,等.山楂叶抗大鼠PMN呼吸爆发谱效关系研究[J].中国中药杂志,2008,33(15)：1884.1884
[153]谭鹏.“谱效关系”应用在中药炮制原理研究中的探讨[G].中华中医药学会第六届中药炮制学术会议论文集,2006.202-204.
[154]孙毅坤.川楝子炮制工艺及质量标准研究[D].北京中医药大学博士研究生学位论文,2004.5.
[155]王凤云.绵茵陈药材的提取、精致工艺及其“谱效结合”评价研究[D].广州中医药大学校硕士学位论文,2006.4.
[156]苏薇薇.沙田柚指纹图谱特征与其药效学关系的研究[D].第一军医大学博士学位论文,2005.4.
[157]马莉.主要基于生物热力学表达的中药板蓝根有效物质筛选与品质评价[D],成都中医药大学博士论文,2006.4.
[158]XIE PS, CHEN SB, LIANG YZ, et al. Chromatographic fingerprint analysis-a rational approach for quality assessment of traditional Chinese herbalmedicine [J]. J Chromatogra,2006,12 (1):171-175.
[159]KO-TA CHIANG, NUN-MINGLIU, CHIH-CHUNG CHOU. Machining parameters optimization on the die casting process of magnesium alloy using thegrey-based fuzzy algorithm[J].Int J Adv Manuf Technol,2008,38 (9): 229-237.
[160]李峰,张振秋,康廷国.灰关联度法评价鹿鞭药材质量的研究[J].中药材,2008,31(2)：189.192.
[161]吴忠,郑少珠.浙贝母、川贝母微量元素灰关联度分析及鉴别分类[J].中药材,1997,20(6)：291—292.
[162]周彤,刘菲菲.新灰色关联分析法评价水牛角、黄牛角与广角的氨基酸组成相似性[J].数理医药学杂志,2001,14(6)：557-559.
[163]吴忠,苏薇薇.中药连翘质量的灰色模式识别研究[J].中药材,2000,23(9)：536-537.
[164]刘向东,张阳,鹿茸品种鉴定与质量评价的研究近况[J].辽宁中医药大学学报,2008,10(7)：126—128.
[165]李峰,张振秋,李可强,等.动物药材质量评价模式研究[C].中国药学大会暨第九届中国药师周论文集.长沙：中国药学会,2009.
[166]李峰,张振秋,康廷国.灰关联度法评价鹿鞭药材质量的研究[J].中药材,2008,31(2)：189-192.
[167]李峰,张振秋,李可强,等.基于灰色关联分析的全蝎、蜈蚣药材商品质量评价研究[J].中成药,2010,32(12)：2118-2120.
[168]李峰,刘春丽,张振秋,等.灰色关联度分析法评价僵蚕药材质量研究[J].辽宁中医杂志,2011,38(2)：203-205.
[169]朱诗塔,雷鹏,李新巾,等.掌叶大黄不同炮制品指纹图谱与其止血作用的灰关联度分析[J].中南药学,2009,7(1)：55.58.
[170]王微.黄芩指纹图谱与药效学研究[D].北京中医药大学硕士研究生论文,2009,5.
[171]刘瑜彬,王晖,唐晓峰,等.灰关联聚类法评价补中益气汤对大鼠气虚发热的效果[J].中国实验方剂学杂志,2010,16(18)：124—126.
[172]陈跃飞,喻嵘.葶苈大枣桑白皮汤组方的灰关联分析[J].湖南中医药大学 学报,2009,29(1)：35-37.
[173]曾建红,陈旭,潘艳薇,等.莪术醇的研究进展[J].中药材,2008,31(1)：168.170.
[174]陈旭,曾建红.广西莪术挥发油化学成分的研究[J].药物生物技术,2008,15(4)：324-326.
[175]陈旭,曾建红.光照强度对广西莪术挥发油及莪术醇含量的影响[J].广西植物,2008,23(5)：378-380.
[176]杨丰庆,李绍平,陈莹,等.不同来源莪术中莪术醇、吉马酮、莪术二酮GC-定量分析[J].药物分析杂志,2005,25(7)：827.829.
[177]WU B, ZHU JS, ZHANG Y, et al. Predictive value of MTT assay as an in vitro chemosensitivity testing for gastric cancer:one institution's experience[J]. World JGastroenterol,2008,14 (19):3064-3068.
[178]BLUMENTHAL RD, GOLDENBERG DM. Methods and goals for the use of in vitro and in vivo chemosensitivity testing [J]. Mol Biotechnol,2007,35 (2): 185-197.
[179]PERFETTO SP, CHATTOPADHYAY PK, ROEDERER M. Seventeen-colour flow cytometry:unravelling the immune system[J]. Nat Rev Immunol,2004, 4 (8):648-655.
[180]INOUE T, NASU Y, TSUSHIMA T, et al. Chromosomal numerical aberrations of exfoliated cells in the urine detected by fluorescence in situ hybridization: clinical implication for the detection of bladder cancer[J]. Urol Res,2000,28 (1):57-61.
[181]MANS DR, ROEHAAB, SEHWARTSMANN G. Anti-cancer drugs diseovery and development in Brazil:Targeted plant collection as a Rational strategy to aequire candidate anti-cancer compounds [J].Oncologist,2000,5(3):185-198.
[182]SAKUDA S, TAMURA S, YAMADAA, et al. NF-kappaB activation in non-parenchymal liver cells after partial hepatectomy in rats:possible involvement in expression of heparin-binding epidermal growth factor-like growth factor[J].J hepatol,2002,36 (4); 527-533.
[183]赵同伟,陈小毅,李飞虹,等.鼻咽癌恶性病变中NF-κB, C-myc和hTERT等表达及意义[J].广东医学院学报,2005,23(1)：17—19.
[184]邓聚龙.灰色系统基本方法[M].武汉：华中理工大学出版社,1987.17.
[185]于萍,李克.使用Microsoft Excel进行数据的灰关联分析[J].微型电脑应用,2011,27(3)：29-31.
[186]陆兔林.中药莪术有效物质及饮片规范化研究[D].南京：南京中医药大学,2003.5.
[2]WILSON B, ABRAHAM G, MANJU VS, et al. Antimicrobial activity of Curcuma zedoaria and Curcuma malabarica tubers[J]. Journal of Ethnopharmacology,2005,99 (1):147-151
[3]国家药典委员会.中华人民共和国药典：一部[S].北京：中国医药科技出版社,2010.257-258.
[4]JIANHONG ZENG, PING DAI, LINGYUN REN, et al. Apoptosis-induced anti-tumor effect of Curcuma kwangsiensis polysaccharides against human nasopharyngeal carcinoma cells[J]. Carbohydrate Polymers,2012,89 (4): 1067-1072.
[5]YANGA FQ, LI ASP, CHENAY, etal. Identification and quantitation of eleven sesquiterpenes in three species of Curcuma rhizomes by pressurized liquid extraction and gas chromatography-mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis,2005,39 (3):552-558.
[6]DI-YA LU, YAN CAO, LING LI, et al. Comparative analysis of essential oils found in Rhizomes Curcumae and Radix Curcumae by gas chromatography-mass spectrometry[J]. Journal of Pharmaceutical Analysis,2011,1 (3):203-207
[7]CHUNHUI DENG, JIE JI, NING LI, et al. Fast determination of curcumol, curdione and germacrone in three species of Curcuma rhizomes by microwave-assisted extraction followed by headspace solid-phase microextraction and gas chromatography-mass spectrometry[J]. Journal of Chromatography A, 2006,1117 (2):115-120.
[8]王琰,王慕邹.莪术的质量研究[J].药学学报,2001,36(11)：849-853.
[9]周欣,梁光义.不同产地莪术挥发油的研究[J].华西药学杂志,2002,17(3)：201—202.
[10]冯磊,陶文沂,敖宗华,等.莪术挥发油的提取工艺和主要成分测定[J].无锡轻工业大学报,2003,22(1)：90-91.
[11]JUN LI, YUE LIU, JIAN-QIANG LI, et al. Chemical Constituents from the Rhizomes of Curcuma kwangsiensis[J]. Chinese Journal of Natural Medicines, 2011,9 (5):329-333.
[12]MOHAMED BOUAZIZ, THABET YANGUI, SAMI SAYADI, et al. Disinfectant properties of essential oils from Salvia officinalis L.cultivated in Tunisia[J]. Food and Chemical Toxicology,2009,47 (11):2755-2760.
[13]WENQIANG GUAN, SHUFEN LI, RUIXIANG YAN, et al. Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods[J].Food Chemistry,2007,101 (4): 1558-1564.
[14]陈伟,吴畏,黄开华,等.超临界流体技术在生物资源活性成分萃取中的应用及展望[J].上海农业学报,2009,25(1)：135—138
[15]王娟,刘汉清.不同提取方法对莪术油提取的影响[J].安徽医药,2007,11(2)：114-115.
[16]陈淑莲,游静,王国俊.超临界流体萃取分析蓬莪术挥发性成分[J].中草药,2000,31(2)：902-903.
[17]刘娜,余德顺,代明权,等.蓬莪术挥发油提取技术的研究及其化学成分分析[J].西南师范大学学报(自然科学版),2002,27(3)：430-432.
[18]游剑,于英伟,李青坡,等.莪术油微球的含量测定[J].中成药,2005,27(1)：25-28.
[19]王婷,李铁福,林绍强,等.莪术醇的分离鉴定及含量测定[J].中华中医药学刊,2008,26(5)：1018-1020.
[20]ZHU YE, ZHU XP. Determination of curcumol in Rhizoma Curcumae By GC[J]. Zhongguo Zhong Yao Za Zhi,2006,31 (5):389-390.
[21]朱亚尔,朱晓平.毛细管色相谱法测定莪术中莪术醇的含量[J].中国中药杂 志,2006,31(5)：389-390.
[22]魏福祥,邓小丽,陈晓,等.气相色谱法测定莪术挥发油中榄香烯含量[J].河北科技大学学报,2008,26(3)：219-221.
[23]项秀娣,陈素红,楼招欢.气相色谱法测定不同方法提取的莪术挥发油中β.榄香烯的含量[J].浙江中医药大学学报,2011,35(3)：397-399.
[24]乔丽,王静,袁子民RP-HPLC法测定β-榄香烯的含量及有关物质[J].中国现代药物应用,2010,4(5)：1—3.
[25]李成网,王唯红.高效液相色谱法测定莪术油中莪术二酮和牦牛儿酮的含量[J].中国实验方剂学杂志,2007,13(3)：12—14.
[26]孙艳涛,刘姝畅,张振秋.HPLC波长切换法测定莪术残油中莪术二酮莪术醇和吉马酮的含量[J].中华中医药学刊,2010,28(2)：391—392.
[27]边海疆,杜明荦,张振秋.高效液相色谱法测定莪术残油软膏剂中莪术醇、吉玛酮含量[J].中医学报,2010,25(6)：2230-2232.
[28]贾东明,姜义娜,薛胜霞.反相高效液相法测定莪术油凝胶剂中莪术醇和吉马酮含量[J].温州医学院学报,2007,37(5)：500-501.
[29]宋爱莉,殷玉琨.莪术油干预治疗肿瘤的研究及应用概况[J].山东中医药大学学报,2008,32(2)：172—174.
[30]赵华,汤为学.莪术油对人子宫内膜癌细胞株RL-95-2抑制作用的体外研究[J].实用妇产科杂志,2006,22(3)：158—160.
[31]宋利琼,张昌菊.莪术油联合干扰素对小鼠宫颈癌端粒酶活性和细胞凋亡的影响[J].上海中医药杂志,2006,40(7)：68-69
[32]宋爱莉,张敬涛,李静蔚,等.莪术油对乳腺癌癌前病变造模大鼠血液流变学及乳房微循环的影响[J].中华中医药学刊,2008,26(3)：458-460.
[33]WU WY, LUO YJ, CHEN JH, et al. Image cytometric DNA analysis of hepaticcarcinomas carried by mice treaed by curcuma aramatica oil [J]. Chin J IntegrTradit West Med Liver Dis,2009,9 (1):18-20.
[34]SHEN LC, WU WY, ZHANG WB, et al. Effect of curcuma aramatica oil on proliferating cell nuclear antgen of hepatoma in mice [J]. World Chin J Digest, 2007,7 (2):56-157.
[35]唐渊,李晓辉.莪术提取物对肝癌细胞系HepG2的抗癌作用及机制研究[J].中国药理学通报,2007,23(6)：790-794.
[36]JIAN ZHANG, TAO ZHANG, XINYU TI, et al. Curcumin promotes apoptosis in A549/DDP multidrug-resistant human lung adenocarcinoma cells through an miRNA signaling pathway[J].Biochemical and Biophysical Research Communications,2010,399 (1):1-6.
[37]盛辉,苑春莉,王医术.莪术油对肺癌SPC2A4细胞株的体外抑制作用[J].江西中医学院学报,2006,18(5)：51.52.
[38]徐立春,边可君,卜平,等.莪术醇修饰的肿瘤疫苗抗MFC效应的研究[J].现代肿瘤医学,2006,14(11)：1357.1359.
[39]湛学军,徐燕萍,谢大泽,等.发光法分析莪术油等中药对肝、胃癌细胞增殖活性抑制作用的实验研究[J].中国肿瘤临床,2007,34(1)：30-33.
[40]周 红,孙海,钟毅.莪术油对白血病细胞株的体外抑制作用[J].广州中医药大学学报,2004,21(2)：131.133.
[41]周红,钟毅,王沁,等.莪术油对白血病细胞体外净化作用的实验研究[J].华西药学杂志,2004,19(4)：256-260.
[42]李传伟,徐英萍,苗 芳,等.莪术油抗小鼠结肠癌效应的实验研究[J].泰山医学院学报,2005,26(2)：89-91.
[43]冯刚,黄涛,卢宏达,等.莪术油注射液对小鼠移植性S180肉瘤血管形成的抑制作用[J].肿瘤研究与临床,2005,17(4)：233-235.
[44]程剑华,刘伟胜,吴万垠,等.中药莪术油肝动脉灌注治疗原发性肝癌的临床研究[J].中医杂志,1999,40(1)：25-26.
[45]程剑华,常钢,吴万垠,等，莪术油和化疗药对照肝动脉灌注栓塞治疗原发性肝癌的临床研究[J]_中国中西医结合杂志,2001,21(3)：165—167.
[46]徐凯,罗海英,李柳宁,等.中药灌注栓塞为主综合治疗原发性肝癌的临床研究[J].中国中西医结合杂志,2005,25(4)：299-302.
[47]赵勤萍,张荣.浅谈中药有效部位研究对中药产业现代化的影响[J].山西中医,2006,22(3)：48-50.
[48]王智民.中药行业发展急需解决的关键问题分析[J1.中国中药杂志,2006, 31 (1)：81—84.
[49]万明珠,梁逸曾,赵晨曦,等.温莪术挥发性化学成分分析和指纹图谱研究[J].时珍国医国药,2009,20(10)：2428-2429.
[50]QIN HL, DENG AJ, DU GH, et al. Fingerprinting analysis of Rhizoma chuanxiong of commercial types using 1H nuclear magnetic resonance spectroscopy and high performance liquid chromatography method [J]. J Integr Plant Biol,2009,51 (6):537-44.
[51]林文津,徐榕青,张亚敏,等.莲子心提取物毛细管电泳指纹图谱研究[J].中国医药生物技术,2009,4(5)：345-348.
[52]刘和平,谢培山,田润涛.柴胡属药材皂苷高效薄层色谱指纹图谱的研究[J].中药新药与临床药理,2008,19(1)：38-42.
[53]张桂芝.肉桂的紫外光谱鉴定研究[J].时珍国医国药,2005,16(10)：951.953.
[54]牟新利,高鹏,付川.三维荧光中药指纹图谱研究[J].光谱实室,2010,27(1)： 180.183.
[55]韩明霞,周群,李全宏,等.不同产地葛根红外光谱的三级鉴定[J].光谱学与光谱分析,2009,7(2)：1851.1855.
[56]孙国祥,孙丽娜,毕开顺.基于整体化学键振动和价电子跃迁的光谱指纹定量法鉴定麻黄质量[J].中南药学,2010,8(1)：52-57.
[57]孙国祥,胡珊,金杰,等.中药X射线衍射指纹图谱专家系统网格(TCM-XFP-ESG)构建与应用[J].中南药学,2009,7(10)：766-769.
[58]杨红玲,吴鸣建,沈国鹏.射干的X射线衍射Fourier指纹图谱鉴定[J].时珍国医国药,2010,21(1)：181.182.
[59]刘红,邹童,张连华,等.益智挥发油的气相指纹图谱的研究[J].安徽农业科学,2008,36(36)：15961-15962.
[60]刘东辉,黄月纯,张子龙,等.薄荷饮片挥发油气相色谱指纹图谱研究[J].中国药业,2011,20(4)：28-30.
[61]孙玲,杨倩,王金虎,等.柴胡挥发油毛细管GC指纹图谱研究[J].中国药物警戒,2010,8(8)：462-464.
[62]黄天来,洪馨,工宁生.生姜挥发油成分气相色谱指纹图谱研究[J1.中药新药与临床药理,2003,14(2)：123.124.
[63]丁平,曾元儿,何智健,等.不同产地阳春砂挥发油气相色谱指纹图谱研究[J].中国药学杂志,2004,39(6)：418-420.
[64]ZENG JH, XU GB, CHEN X. Application of the chromatographic fingerprint for quality control of essential oil from GuangXi Curcuma kwangsiensis[J]. Med Chem Res,2009,18 (2):158-165.
[65]HU LF, LI SP, CAO H, et al. GC-MS fingerprint of Pogostemon cablin in China[J]. Journal of Pharmaceutical and Biomedical Analysis,2006,42 (2): 200-206.
[66]魏刚,林双峰,方永奇.气相色谱-质谱联用法分析石菖蒲水煎液主要化学成分[J].广州中医药大学学报,2005,22(2)：147—149.
[67]周欣,李章万,王道平,等GC-MS法建立温莪术挥发油指纹图谱研究[J].中国中药杂志,2004,29(12)：1138—1141.
[68]周婧,都晓伟,马宏跃,等.西洋参药材高效液相色谱法指纹图谱的研究[J].时珍国医国药,2006,17(12)：2381-2383.
[69]ZHANG TT, WU Y, HANG TJ. Study on HPLC fingerprint of flavonoids from Houttuynia cordataby comparing with fingerprint reference [J]. Zhong Yao Cai, 2009,32 (5):687-90.
[70]陶敬奇,闻莹莹,王 辉,等.五指毛桃指纹图谱的高效液相色谱-质谱联用研究[J].华南师范大学学报(自然科学版),2011,3(3)：77-79.
[71]张敏,王义明,罗国安.日本救心丹多维指纹图谱研究[J].药学学报,2006,41(12)：1161.1165.
[72]兰作平,曲中堂.试论中药指纹图谱的研究现状及其研究过程的标准化[J].西部药学,2006,3(2)：71-72.
[73]孙国祥,宋文景.中药的毛细管电泳指纹图谱的研究方法[J].中南药学,2008,6(6)：752-754.
[74]谷巍,巢建国,刘训红,等.安徽道地药材牡丹皮高效毛细管电泳指纹图谱研究[J].中国实验方剂学杂志,2011,17(7)：58—60.
[75]YISUN, TAO GUO, YIN SUI, et al. Fingerprint analysis of Flos Carthami by capillary electrophoresis[J]. Journal of Chromatography B,2003,792 (2): 147-152.
[76]张丽艳,罗君,李健,等.吴茱萸药材薄层色谱指纹图谱研究[J].中国实验方剂学杂志,2011,17(12)：72-74.
[77]冯毅凡,周漩,郭晓玲.向量夹角法评价不同产地与商品来源的高良姜挥发油指纹图谱相似度[J].中药材,2006,29(1)：10-13.
[78]王龙星,梁鑫淼.一种评价中药色谱指纹谱相似性的新方法：向量夹角法[J].药学学报,2002,37(9)：713-717.
[79]王成哲,卢佩,沈鸿章.基于模糊聚类的中药对照指纹图谱研究[J].天津科技大学学报,2007,22(2)：62-65.
[80]袁鹏,袁敏,胡跃明.模糊聚类分析法在郁金裂解色谱测定指纹图谱中的应用研究[J].计算机与应用化学,2005,22(3)：201—205.
[81]田兰,毕开顺,赵少春.白术的化学模式识别[J].中国中药杂志,2003,28(2)：52-55.
[82]蒋建平.色谱指纹图谱识别的人工神经网络方法对比研究[J].广东化工,2011,38(2)：58-59.
[83]汤丹,许毅,吴秋云.广藿香指纹图谱解析的人工神经网络方法研究[J].中药材,2004,27(7)：534-536.
[84]韦记青,孔德鑫,唐辉,等.基于主成分分析和聚类分析的野生战骨红外指纹图谱比较研究[J].广西植物,2011,31(4)：559-563.
[85]李晓红,熊志立,虞明阳,等.基于主成分分析的骨碎补药材乙醇和环己烷提取物的高效液相色谱指纹图谱及指标成分的定量分析[J].色谱,2009,27(4)：453-457.
[86]郝燕,董鸿晔,姜楠,等.基于主成分分析的中药色谱指纹图谱多维多息特征数据挖掘方法研究[J].中南药学,2007,5(3)：267-272.
[87]高华荣,孙相民.中药指纹图谱在现代中药制剂中的应用[J].中国实用医药,2009,4(14)：240-241.
[88]朱善岚,黄品芳.HPLC法测定莪术不同炮制品中姜黄素含量[J].海峡药学,2007,19(3)：52—53.
[89]罗春兰,吴爱琴.不同品种莪术挥发油成分GC-MS分析[J].广东药学,2005,15(2)：10.11.
[90]王智民.中药行业发展急需解决的关键问题分析[J].中国中药杂志,2006,31(1)：81.84.
[91]YI JL, GAO L, HUANG XD, et al. Nasopharyngeal carcinoma treated by radical radiotherapy alone:ten year experience in a single institute [J]. Int J Radiat Oncol Biol Phys,2006,65 (1):161-168.
[92]CHENG SH, TSAI SY, YEN KL, et al. Prognostic significance of parapharyngeal space venous plexus and marrow involvement:potential landmarks of dissemination for stage Ⅰ-Ⅲ nasopharyngeal carcinoma [J]. Int J Radiat Biol Phys,2005,61 (2):456-465.
[93]HARlOZINSKA A. Progress in molecular mechanisms of tumor metastasis and angiogenesis[J].Anticancer Res,2005,25 (5):3327-3333.
[94]DIVYA CS, PILLAI MR. Antitumor action of curcumin in human papillomavirus associated cells involves downregulation of viral oncogenes, prevention of NFκB and AP-1 translocation, and modulation of apoptosis[J]. Molecular carcinogenesis,2006,45 (5):320-332.
[95]DEISY HH, GARCIA OP, JORGE L, et al. The contribution of fruits and vegetables to dietary intake of polyphenols and antioxidant capacity in a Mexican rural diet:Importance of fruit and vegetable variety [J]. Food Research International,2011,44 (5):1182-1189.
[96]KATARZYNA P, IRENA BB, MARIOLA M, et al. Changes in male reproductive system and mineral metabolism induced by soy isoflavones administered to rats from prenatal life until sexual maturity [J]. Nutrition,2011, 27 (3):372-379.
[97]PATRICIA ALSP, JOAO ANL, VICTOR APF.Antioxidant protection of low density lipoprotein by procyanidins:structure/activity relationships Original Research Article[J]. Biochemical Pharmacology,2003,66(6):947-954..
[98]PHILIPP SAIKO, AKOS SZAKMARY, WALTER JAEGER, et al. Resveratrol and its analogs:Defense against cancer, coronary disease and neurodegenerative maladies or just a fad? Review Article[J]. Mutation Research/Reviews in Mutation Research,2008,658 (1):68-94.
[99]XU CHEN, JIANHONG ZENG. Investigation on Influential Factors of Volatile Oil and Main Constituent Content from Curcuma kzoangsiensis S. G. Lee C. F. Liang. In Guangxi Producing Areas[J]. Modern Applied Science,2008,2 (1): 53-57.
[100]SEN R, BALTIMORE D. Mufiple nuclear factors interact with the im-munoglobulinenhancersequencees[J]. Cell,2006,46 (5):705-716.
[101]LEE DK, KANG JE, PARK HJ, et al. FBI-1 enhances transcription of the nuclear factor-kappaB (NF-kappaB)-responsive E-selectin gene by nuclear localization of the p65 subunitofNF-kappaB[J]. JBiolChem,2005,280 (30): 27783-22791.
[102]MATSUOS, YAMAZAKIS, TAKESHIGE K, eta.l Crucial roles of binding sites for NF-kappaB and C/EBPs in IkappaB-zeta-mediated transcriptional activation[J]. Biochem J,2007,405 (3):605-609.
[103]KARIN M, CAO Y, GRETEN FR, et al. NF-kappaB in cancer:from innocent bystander to major culprit [J].Nat Rev Cancer,2002,12 (2):301-310.
[104]KIM HJ, HAWKE N, BALDWIN AS. NF-kappa B and IKK as therapeutic targets in cancer [J]. Cell Death Differ,2006,13 (2):738-747.
[105]DOLCET X, LLOBET D, PALLARES J, et al. NF-kB in development and progression of human cancer [J]. Virchows Arch,2005,446 (5):475-482.
[106]SALVATORE C, CAMARDA G, MGGSI CA, et al. NF-kappa B activation contributes to anthracycline resistance pathway in human ovarian carcinoma cell line A2780[J]. Int J Oncol,2005,27 (3):799-806.
[107]SIEBENLISTU, BROWN K, CLAUDIO E. Control of lymphocyte developmentby nuclear factor-kappaB [J]. Nat Rev Immunol,2005,5 (1): 435-445.
[108]BHAKAR AL, TANNIS LL, ZEINDLER C, et al. Constitutive NF-κB activity is required for central neuron survival [J]. Neuroscience,2002,22 (9): 8466-8475.
[109]XIAO-LONG HOU, KYOKO TAKAHASHI, KEN TANAKA, et al. Curcuma drugs and curcumin regulate the expression and function of P-gp in Caco-2 cells in completely opposite ways[J]. International Journal of Pharmaceutics,2008, 358 (1):224-229.
[110]DOLCET X, LLOBET D, PALL ARES J, et al. NF-kB in development and progression of human cancer [J]. Virchows Arch,2005,446 (5):475-482.
[111]IRBACH A, GOLD P, BINDER BR, et al. Signalingmolecules of the NF-kappa B pathway shuttle constitutively between cytoplasm and nucleus [J]. J Biol Chem,2009,277 (13):10842-10851.
[112]JASON HTW, VIVIAN WYL, KAZUO UYH, et al. A small molecule inhibitor of NF-κB, dehydroxymethylepoxyquinomicin (DHMEQ), suppresses growth and invasion of nasopharyngeal carcinoma (NPC) cells Original Research Article[J]. Cancer Letters,2010,287 (1):23-32.
[113]PEERARAT THAINA, PATTREEYA TUNGCHAROEN, MALINEE WONGNAWA, et al. Uterine relaxant effects of Curcuma aeruginosa Roxb.rhizome extracts[J]. Journal of Ethnopharmacology,2009,121 (3): 433-443.
[114]CHEN XU, ZHONG HAI-YAN, ZENG JIAN-HONG, et al. The pharmacological effect of polysaccharides from Curcuma Kwangsiensis on the oxidative status and expression of VCAM-1mRNA of thoracic aorta endothelial cell in high-fat-diet rats [J].Carbohydrate Polymers,2008,74 (3):445-450.
[115]胡延维,韩蓉.莪术油注射液的安全性试验研究[J].中国医院药学杂志,2006,26(7)：904-905.
[116]贾晓斌,陈彦,李霞,等.中药复方物质基础研究新思路和方法[J].中华中医药杂志,2008,23(5)：420-422.
[117]贺福元,邓凯文,石继连,等.中药有效成分群组方技术研究若干基础瓶 颈问题及数理特征化思路的提出[J].中药材,2009,32(1)：1.4.
[118]贺福元,邓凯文,邹欢,等.中药复方谱动学与谱效动力学差异性的研究[J].中国中医杂志,2011,36(2)：136—138.
[119]任德权.中药现代化德几个问题[J].中成药,2003,25(1)：1.3.
[120]罗国安,王义明,曹进,等.建立我国现代中药质量标准体系的研究[J].世界科学技术,2002,4(4)：5—11.
[121]李戎, 闫智勇,李文军,等.创建中药谱效关系学[J].中医教育,2002,(21)2：62-64.
[122]宁黎丽,毕开顺,王瑞,等.吴茱萸汤药效物质基础的方法学研究[J].药学学报,2000,35(2)：131.134.
[123]周立东.建议在天然药物研究中建立“定量组效关系”(QCAR)概念[J].世界科学技术,1999,(1)2：33—34.
[124]杨东风,梁宗锁.中药指纹图谱研究进展[J].中国药房,2007,18(6)：467-470.
[125]刘荣霞,叶敏,果德安.中药质量控制研究的思路与方法[J].中国天然药,2006,4(5)：332-337.
[126]王微.黄芩指纹图谱及谱效关系研究[M].北京：北京中医药大学,2009.43-61.
[127]李洁,孙静.实现中药质量控制突破的思考[J].中华中医药学刊,2007,25(8)：1739.1740
[128]崔红华,邱夏.中药质量标准现代化研究发展趋势[J].中国中医药信息杂志,2007,14(6)：6-7
[129]李萍,刘耀.中药指纹图谱的研究现状和发展趋势[J].中国药业,2009,18(8)：19-21.
[130]李戎,闫智勇,李文军,等.“谱效关系”研究是中药质量与药效标准规范的关键环节[J].医药简讯,2002,10(6)：18—20.
[131]LII SHENG-QING, LIU YUN-SHENG, YANG HUI, et al. Observation on gene augmentation of cytosine deaminase to neural stem cells and its expression in vitro [J]. Chiuese Journal of Clinical Rehabilitation,2005,9 (1):213-215.
[132]于秀琳,任雪松.多元统计分析[M].北京：中国统计出版社,1999.62.
[133]许良, 毕开顺.多元线性回归分析法在蒙药森登汤谱效关系解析中的应用[J].计算机与应用化学,2008,25(10)：1189.1192.
[134]YANG DE-GANG, CHEN GUO, WANG HUI, et al. Learning Vector Quantization Neural Network Method for Network Intrusion Detection[J]. Wuhan University Journal of Naturnal Sciences,2007,23 (1):147-150.
[135]邓聚龙.灰色系统基本方法[M].武汉：华中科技大学出版社,2005.23—30.
[136]许虹,黄丹,郑元.模糊数学在茶多酚药理学研究中的应用[J].数理医药学杂志,2002,15(1)：87-89.
[137]KONG ZHENG-MIN, ZHONG LIANG, ZHU GUANG-XI, et al. Differential multiuser detection using a novel genetic algorithm for ultra-wideband systems in lognormal fading channel [J]. Comput & Electron,2011,12(9):754-765.
[138]杨如乃,胡志忠,卢健.生物嗅觉神经系统模型的模拟与分析[J].生物医学工程研究,2007,25(3)：131.136.
[139]YANG XIN-LING, FU JUN, LUO ZHENG-GUO. Tea classification based on artificial using bionic olfactory neural networks [C]. Proceedings of Third International Symposium on Neural Networks,2006,397 (3):343-348.
[140]马丽,杨琳,金印彬,等.细胞外低钾对心室肌组织钠通道电导影响的计算机仿真研究[J].生物医学工程学杂志,2009,26(1)：1—3.
[141]张锦,李司铎,王如龙,等.KⅢ模型动力学特性研究[J].湖南大学学报,2008,35(3)：76-79.
[142]张强,张霜红,钱建明,等.用灰关联聚类法评价中国少数民族人口素质[J].现代预防医学,2003,30(5)：43-45.
[143]赵筱萍,范骁辉,余杰,等.一类基于组效关系神经网络模型的中药药效预测方法[J].中国中药杂志,2004,29(11)：1082-1084.
[144]刘雪松,施朝晟,程翼宇,等.基于自组织映射神经网络的中药注射剂质量快速鉴别方法[J]_分析化学,2007,35(10)：1483.1486.
[145]范骁辉,程翼宇.中药复杂组效关系的变结构神经网络辨识方法[J].高等学校化学学报,2004,25(11)：2004.2009.
[146]RUDOLPH MERWE, TODD KL, ZHENGDONG LU, et al.Fast neural network surrogates for very high dimensional physics-based models in computational oceanography Original Research Article[J].Neural Networks, 2007,20 (4):462-478.
[147]周立艳,梁生旺,王淑美,等.牡丹皮高效液相色谱“药效指纹图谱”研究[J].时珍国医国药,2008,19(6)：1337—1339.
[148]刘荣霞,叶敏,果德安.中药质量控制研究的思路与方法[J].中国天然药,2006,4(5)：332-337.
[149]吴悦涛.指纹图谱在制定中药质量标准研究中出现的问题与对策[J].黑龙江医药,2007,20(1)：64-65.
[150]薛飞群.中药复方TF-103抗球虫活性与指纹图谱组效关系研究[J].中国兽药杂志,2006,40(3)：1—5.
[151]钱俊,尹莲.加味四妙丸有效部位群HPLC指纹图谱归属分析及谱效关系研究[J].世界科学技术,2007,9(1)：40-43.
[152]刘荣华,余伯阳,陈兰英,等.山楂叶抗大鼠PMN呼吸爆发谱效关系研究[J].中国中药杂志,2008,33(15)：1884.1884
[153]谭鹏.“谱效关系”应用在中药炮制原理研究中的探讨[G].中华中医药学会第六届中药炮制学术会议论文集,2006.202-204.
[154]孙毅坤.川楝子炮制工艺及质量标准研究[D].北京中医药大学博士研究生学位论文,2004.5.
[155]王凤云.绵茵陈药材的提取、精致工艺及其“谱效结合”评价研究[D].广州中医药大学校硕士学位论文,2006.4.
[156]苏薇薇.沙田柚指纹图谱特征与其药效学关系的研究[D].第一军医大学博士学位论文,2005.4.
[157]马莉.主要基于生物热力学表达的中药板蓝根有效物质筛选与品质评价[D],成都中医药大学博士论文,2006.4.
[158]XIE PS, CHEN SB, LIANG YZ, et al. Chromatographic fingerprint analysis-a rational approach for quality assessment of traditional Chinese herbalmedicine [J]. J Chromatogra,2006,12 (1):171-175.
[159]KO-TA CHIANG, NUN-MINGLIU, CHIH-CHUNG CHOU. Machining parameters optimization on the die casting process of magnesium alloy using thegrey-based fuzzy algorithm[J].Int J Adv Manuf Technol,2008,38 (9): 229-237.
[160]李峰,张振秋,康廷国.灰关联度法评价鹿鞭药材质量的研究[J].中药材,2008,31(2)：189.192.
[161]吴忠,郑少珠.浙贝母、川贝母微量元素灰关联度分析及鉴别分类[J].中药材,1997,20(6)：291—292.
[162]周彤,刘菲菲.新灰色关联分析法评价水牛角、黄牛角与广角的氨基酸组成相似性[J].数理医药学杂志,2001,14(6)：557-559.
[163]吴忠,苏薇薇.中药连翘质量的灰色模式识别研究[J].中药材,2000,23(9)：536-537.
[164]刘向东,张阳,鹿茸品种鉴定与质量评价的研究近况[J].辽宁中医药大学学报,2008,10(7)：126—128.
[165]李峰,张振秋,李可强,等.动物药材质量评价模式研究[C].中国药学大会暨第九届中国药师周论文集.长沙：中国药学会,2009.
[166]李峰,张振秋,康廷国.灰关联度法评价鹿鞭药材质量的研究[J].中药材,2008,31(2)：189-192.
[167]李峰,张振秋,李可强,等.基于灰色关联分析的全蝎、蜈蚣药材商品质量评价研究[J].中成药,2010,32(12)：2118-2120.
[168]李峰,刘春丽,张振秋,等.灰色关联度分析法评价僵蚕药材质量研究[J].辽宁中医杂志,2011,38(2)：203-205.
[169]朱诗塔,雷鹏,李新巾,等.掌叶大黄不同炮制品指纹图谱与其止血作用的灰关联度分析[J].中南药学,2009,7(1)：55.58.
[170]王微.黄芩指纹图谱与药效学研究[D].北京中医药大学硕士研究生论文,2009,5.
[171]刘瑜彬,王晖,唐晓峰,等.灰关联聚类法评价补中益气汤对大鼠气虚发热的效果[J].中国实验方剂学杂志,2010,16(18)：124—126.
[172]陈跃飞,喻嵘.葶苈大枣桑白皮汤组方的灰关联分析[J].湖南中医药大学 学报,2009,29(1)：35-37.
[173]曾建红,陈旭,潘艳薇,等.莪术醇的研究进展[J].中药材,2008,31(1)：168.170.
[174]陈旭,曾建红.广西莪术挥发油化学成分的研究[J].药物生物技术,2008,15(4)：324-326.
[175]陈旭,曾建红.光照强度对广西莪术挥发油及莪术醇含量的影响[J].广西植物,2008,23(5)：378-380.
[176]杨丰庆,李绍平,陈莹,等.不同来源莪术中莪术醇、吉马酮、莪术二酮GC-定量分析[J].药物分析杂志,2005,25(7)：827.829.
[177]WU B, ZHU JS, ZHANG Y, et al. Predictive value of MTT assay as an in vitro chemosensitivity testing for gastric cancer:one institution's experience[J]. World JGastroenterol,2008,14 (19):3064-3068.
[178]BLUMENTHAL RD, GOLDENBERG DM. Methods and goals for the use of in vitro and in vivo chemosensitivity testing [J]. Mol Biotechnol,2007,35 (2): 185-197.
[179]PERFETTO SP, CHATTOPADHYAY PK, ROEDERER M. Seventeen-colour flow cytometry:unravelling the immune system[J]. Nat Rev Immunol,2004, 4 (8):648-655.
[180]INOUE T, NASU Y, TSUSHIMA T, et al. Chromosomal numerical aberrations of exfoliated cells in the urine detected by fluorescence in situ hybridization: clinical implication for the detection of bladder cancer[J]. Urol Res,2000,28 (1):57-61.
[181]MANS DR, ROEHAAB, SEHWARTSMANN G. Anti-cancer drugs diseovery and development in Brazil:Targeted plant collection as a Rational strategy to aequire candidate anti-cancer compounds [J].Oncologist,2000,5(3):185-198.
[182]SAKUDA S, TAMURA S, YAMADAA, et al. NF-kappaB activation in non-parenchymal liver cells after partial hepatectomy in rats:possible involvement in expression of heparin-binding epidermal growth factor-like growth factor[J].J hepatol,2002,36 (4); 527-533.
[183]赵同伟,陈小毅,李飞虹,等.鼻咽癌恶性病变中NF-κB, C-myc和hTERT等表达及意义[J].广东医学院学报,2005,23(1)：17—19.
[184]邓聚龙.灰色系统基本方法[M].武汉：华中理工大学出版社,1987.17.
[185]于萍,李克.使用Microsoft Excel进行数据的灰关联分析[J].微型电脑应用,2011,27(3)：29-31.
[186]陆兔林.中药莪术有效物质及饮片规范化研究[D].南京：南京中医药大学,2003.5.