益母草对雌激素干预下大鼠子宫内膜ER、PR表达的影响
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摘要
目的：本实验旨在观察雌性未成年大鼠在单一雌激素干预下不同时间段子宫内膜组织的病理变化特征,通过免疫组织化学方法检测大鼠子宫内膜ER、PR的表达,探讨用中药益母草对无排卵性功血大鼠子宫内膜的修复作用机制。
方法：选用雌性未成年大鼠,将大鼠随机分为2组：空白对照组和模型组。空白对照组给予生理盐水连续灌胃5周；模型组给予戊酸雌二醇灌胃3周,即全周期雌激素干预。3周后将模型组分为雌激素组、益母草组和模型组；模型组用生理盐水灌胃2周,雌激素组给予戊酸雌二醇灌胃2周,益母草组用益母草颗粒灌胃2周。通过不同时间段取大鼠的子宫组织,观察不同状态下使用雌激素干预以及使用药物治疗子宫内膜组织的病理变化；运用免疫组化法测定雌激素干预下和药物治疗下对子宫内膜ER、PR的影响。
结果：第1-3周空白对照组与模型组相比,模型组子宫内膜ER、PR的表达均逐渐增高,空白对照组子宫内膜ER、PR的表达无明显变化,均低于模型组,差异有统计学意义(P<0.05)；第4-5周在药物的治疗作用下,益母草组和雌激素组的ER、PR表达均有所降低,差异有统计学意义(P<0.05)；益母草组与雌激素组比较无明显差异(P>0.05)。
结论：实验结果表明,对于单一雌激素干预下的大鼠,在戊酸雌二醇干预的1-3周子宫内膜中ER、PR的水平表现不同程度的增高；在4-5周药物的治疗作用下,益母草组和雌激素组大鼠子宫内膜组织中ER、PR水平降低,说明益母草和戊酸雌二醇均能起到良好的治疗作用。
Objective:This study was designed to observe the pathological changes characteristic of female immature rats in different of times in the intervention of a single estrogen endometrial tissue continuity, the rat endometrial ER and PR expression detected by immunohistochemical methods to explore the use of Chinese medicine leonurus heterophylluy sweet DUB rat endometrial repair mechanism.
Method:Female immutare rats, the rats were randomly divided into two groups:blank control group and model group. The blank control group was given physiological saline stomach for irrigation five weeks; the model group was given estradiol valerat three weeks, the namely complete cycle estrogen intervention. Three weeks after the model group will be divided into estrogen group, the group and the model group; Model group with saline water irrigation stomach two weeks, estrogen group for estradiol valerat stomach to two weeks, the group used the filling particles stomach two weeks. Through the different period take the rat uterine tissue, observe different state intervention and the use of drugs used estrogen to treat endometrial the pathological changes of the organization; Use immunohistochemical method to determine the intervention of estrogen and medication of endometrial ER, PR influence.
Results:The model group endometrial ER, PR expression is higher, blank control group endometrial ER, PR expression no change, the blank control group were all lower than model group, the difference was statistically significant (P<0.05); Four to five weeks in drug treatment effect, the group and estrogen group ER, PR express reduced, the difference was statistically significant (P<0.05); The group and estrogen group there were no significant difference (P>0.05).
Conclusions:The experimental results show that, for a single estrogen with rats, fill in the one to three inlets intervention in the week endometrial ER, PR performance level of different level increased; In four to five weeks drug treatment effect, the group and estrogen rats, endometrial tissue ER, PRreduced level, explain and supplement of motherwort all can play a good inlets treatment function.
方法：选用雌性未成年大鼠,将大鼠随机分为2组：空白对照组和模型组。空白对照组给予生理盐水连续灌胃5周；模型组给予戊酸雌二醇灌胃3周,即全周期雌激素干预。3周后将模型组分为雌激素组、益母草组和模型组；模型组用生理盐水灌胃2周,雌激素组给予戊酸雌二醇灌胃2周,益母草组用益母草颗粒灌胃2周。通过不同时间段取大鼠的子宫组织,观察不同状态下使用雌激素干预以及使用药物治疗子宫内膜组织的病理变化；运用免疫组化法测定雌激素干预下和药物治疗下对子宫内膜ER、PR的影响。
结果：第1-3周空白对照组与模型组相比,模型组子宫内膜ER、PR的表达均逐渐增高,空白对照组子宫内膜ER、PR的表达无明显变化,均低于模型组,差异有统计学意义(P<0.05)；第4-5周在药物的治疗作用下,益母草组和雌激素组的ER、PR表达均有所降低,差异有统计学意义(P<0.05)；益母草组与雌激素组比较无明显差异(P>0.05)。
结论：实验结果表明,对于单一雌激素干预下的大鼠,在戊酸雌二醇干预的1-3周子宫内膜中ER、PR的水平表现不同程度的增高；在4-5周药物的治疗作用下,益母草组和雌激素组大鼠子宫内膜组织中ER、PR水平降低,说明益母草和戊酸雌二醇均能起到良好的治疗作用。
Objective:This study was designed to observe the pathological changes characteristic of female immature rats in different of times in the intervention of a single estrogen endometrial tissue continuity, the rat endometrial ER and PR expression detected by immunohistochemical methods to explore the use of Chinese medicine leonurus heterophylluy sweet DUB rat endometrial repair mechanism.
Method:Female immutare rats, the rats were randomly divided into two groups:blank control group and model group. The blank control group was given physiological saline stomach for irrigation five weeks; the model group was given estradiol valerat three weeks, the namely complete cycle estrogen intervention. Three weeks after the model group will be divided into estrogen group, the group and the model group; Model group with saline water irrigation stomach two weeks, estrogen group for estradiol valerat stomach to two weeks, the group used the filling particles stomach two weeks. Through the different period take the rat uterine tissue, observe different state intervention and the use of drugs used estrogen to treat endometrial the pathological changes of the organization; Use immunohistochemical method to determine the intervention of estrogen and medication of endometrial ER, PR influence.
Results:The model group endometrial ER, PR expression is higher, blank control group endometrial ER, PR expression no change, the blank control group were all lower than model group, the difference was statistically significant (P<0.05); Four to five weeks in drug treatment effect, the group and estrogen group ER, PR express reduced, the difference was statistically significant (P<0.05); The group and estrogen group there were no significant difference (P>0.05).
Conclusions:The experimental results show that, for a single estrogen with rats, fill in the one to three inlets intervention in the week endometrial ER, PR performance level of different level increased; In four to five weeks drug treatment effect, the group and estrogen rats, endometrial tissue ER, PRreduced level, explain and supplement of motherwort all can play a good inlets treatment function.
引文
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[2]石晓敏,顾宁,曹云莉,朱振华,戊酸雌二醇在绝经后妇女取环术前的应用,西南国防医药年,2009,19(5)：518-509
[3]谢贝贝,杨钟莉戊酸雌二醇对建立人工周期的作用,中国妇幼保健杂志,2006,21(12)：1687
[4]王月鹏,赵行字,张慧锋,成年大鼠性周期阴道细胞变化探究,中国科技信息,2007,17：192-193
[5]唐果渝蔡雅琴,KGF对无排型功血子宫内膜上皮细胞ER和PR的调节研究,现代医药卫生,2010,26(21)：3227-3229
[6]张以文,正常子宫出血及功能失调性子宫出血的出血机制,实用妇产科杂志,1998,14(2)：62-64
[7]Sumida C, Pasqualini JR, Antiestrogens antagonize the stimulatory effect of epidermal growth factor on the induction of progesterone receptor in fetal uterine cells in culture, Endocrinology,2005,124(2):591
[8]李晶华,冯力民,ER. PR结构在女性生殖器官的分布,中国妇产科临床杂志,2004,5(6)：475-477
[9]Barkhem T, Nilsson S, Gustafsson JA,Molecular mechanisms physiological consequences and pharmacological implication of estrogen receptor action, American Journal of pharmaco Genomics,2004,4(1):19-28
[10]王欣,成娅,程湘,无排卵功血子宫内膜生长修复相关因素的研究进展,临床军医杂志,2007,35(3)：447-448
[11]MaybinJA, Hirani N, Jabbour HN, Critchley HO, Novel roles for hypoxia and prostaglandin E2 in the regulation of IL-8 during endometrial repair,Am J Pathol, 2011,178(3):1245-56
[12]于君,许俐,子宫内膜局部微环境与功能性子宫出血相关性研究进展,实用医药杂志,2007,9(24)：1119-1120
[13]孙燕,乔福元,徐金下,旷晓聪,无排卵性功能失调性子宫出血患者子宫 内膜雌、孕激素受体及基质金属蛋白酶表达变化的研究,生殖医学杂志,2006,15(5)：301-304
[14]王从斌,从肾论治青春期功能失调性子官出血,辽宁中医学院学报,2002,(4)：293
[15]李坤寅,王慧颖,欧阳慧卿教授治疗崩漏经验介绍,新中医,2005,37(8)
[16]朱丽红,邵文彬,杨家林教授论治崩漏,中医研究,2005,18(1)：56-58
[17]邓高丕,陈莉舒,袁烁,青春期功血出血期的中医证治规律研究, 中国医疗前沿,2009,4(17)：13-14
[18]朱丽红,无排卵型子宫出血的中草药治疗,中国临床医生,2008,31(11)：57-58
[19]王亚芬,崩漏病因与证治文献探讨,中医文献杂志,2009,24-26
[20]翁菊仙,无排卵型功能失调性子宫出血诊断与中西医治疗体会,现代中西医结合杂志,2005,14(19)：2572-2573
[21]朱南孙,中国传统医学在功能失调性子宫出血治疗方面的应用,中国实用妇科与产科杂志,2004,20(4)：207
[22]夏敏,翁双燕,中药人工周期疗法治疗青春期功血51例,实用中医药杂志,2004,20(3)：125
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