结核分枝杆菌c-di-GMP代谢相关蛋白的生物化学研究的功能衰退
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摘要
结核病是严重危害人类健康的传染病,由结核分枝杆菌(Mycobacterium tuberculosis,MTB)慢性感染引起,是目前传染病中仅次于艾滋病的第二号杀手。到目前为止,包括研究最深入的结核分枝杆菌菌株H37Rv在内的多个菌株已经被测序,为我们进行结核分枝杆菌的生物学研究,以及发展新的预防治疗结核方法提供了良好的基础。
环二鸟苷酸(c-di-GMP)是近十余年发现的细菌胞内第二信使,由二分子cGMP通过3′,5?-磷酸二酯键连接而成,在P. aeruginosa、伤寒沙门氏菌(Salmonella typhimurium)等G?细菌和S. aureus等G+细菌中广泛存在。近年来,随着微生物基因组的大规模测序,环二鸟苷酸(c-di-GMP)吸引了越来越多的关注。c-di-GMP在细胞内的水平分别受到含GGDEF结构域(二鸟苷酸环化酶活性)和EAL结构域(磷酸二酯酶活性)蛋白的正向和负向调节,是原核生物中普遍存在的多细胞行为调节因子,通过多种交叉信号网络调控生物膜形成、耐药性、毒力因子表达等生理过程。
Countless millions of people in the world have died from tuberculosis, a chronic infectious disease caused by the tubercle bacillus. The complete genome sequence of the best-characterized Mycobacterium tuberculosis strain, H37Rv, has been determined and analysed in order to improve the understanding of the biology of this slow-growing pathogen and to help finding new prophylactic and therapeutic interventions.
Bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) has come to the limelight as one of the result of the recent advances in microbial genomics.C-di-GMP is known as a novel global second messenger in bacteria. The GGDEF and EAL domain proteins, which are involved in c-di-GMP synthesis and degradation, respectively, are ubiquitous in bacterial genomes. These proteins affect cell differentiation and multicellular behaviour as well as the interactions between the microorganisms and their eukaryotic hosts.
环二鸟苷酸(c-di-GMP)是近十余年发现的细菌胞内第二信使,由二分子cGMP通过3′,5?-磷酸二酯键连接而成,在P. aeruginosa、伤寒沙门氏菌(Salmonella typhimurium)等G?细菌和S. aureus等G+细菌中广泛存在。近年来,随着微生物基因组的大规模测序,环二鸟苷酸(c-di-GMP)吸引了越来越多的关注。c-di-GMP在细胞内的水平分别受到含GGDEF结构域(二鸟苷酸环化酶活性)和EAL结构域(磷酸二酯酶活性)蛋白的正向和负向调节,是原核生物中普遍存在的多细胞行为调节因子,通过多种交叉信号网络调控生物膜形成、耐药性、毒力因子表达等生理过程。
Countless millions of people in the world have died from tuberculosis, a chronic infectious disease caused by the tubercle bacillus. The complete genome sequence of the best-characterized Mycobacterium tuberculosis strain, H37Rv, has been determined and analysed in order to improve the understanding of the biology of this slow-growing pathogen and to help finding new prophylactic and therapeutic interventions.
Bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) has come to the limelight as one of the result of the recent advances in microbial genomics.C-di-GMP is known as a novel global second messenger in bacteria. The GGDEF and EAL domain proteins, which are involved in c-di-GMP synthesis and degradation, respectively, are ubiquitous in bacterial genomes. These proteins affect cell differentiation and multicellular behaviour as well as the interactions between the microorganisms and their eukaryotic hosts.
引文
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