模拟瘤胃条件下苜蓿对粗饲料产气特性和发酵特性的研究
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摘要
本试验通过体外模拟瘤胃装置比较了黑麦草、苜蓿、麦秸、玉米秸、青贮玉米秸等五种粗饲料的产气性能和发酵性能,并研究了苜蓿添加到黑麦草、麦秸、玉米秸、青贮玉米秸中的组合效应。结果表明:黑麦草、苜蓿、麦秸、玉米秸、青贮玉米秸在12h、24h、48h的累计产气量和产气参数a、b、a+b、c差异极显著(P<0.01);五种粗饲料经48h体外培养后的DM、NDF降解率差异极显著(P<0.01),并且苜蓿的DM降解率最高,次之是青贮玉米秸、黑麦草、麦秸、玉米秸;对于NDF的降解率苜蓿最高,次之是黑麦草、青贮玉米秸、玉米秸、麦秸;五种粗饲料对模拟瘤胃内的pH值、NH_3-N浓度都有极显著的影响(P<0.01)。
黒麦草、麦秸、玉米秸、青贮玉米秸中添加0%、25%、50%、75%、100%的苜蓿在12h、24h、48h的累计产气量及其估测产气参数值a、b、a+b、c、DM和NDF降解率之间差异极显著(P<0.01),DM、NDF降解率随苜蓿添加量的增大而增大,并且添加苜蓿的DM、NDF降解率均高于未添加时的降解率。
黑麦草中苜蓿占到25%、50%、75%时,在12h、48h的累计产气量上的组合效应均显著(P<0.05),对于24h的产气量的组合效应,苜蓿添加25%、50%都达到了显著(P<0.05),但添加75%时不显著(P>0.05),产气效应随着添加量的增加正效应降低;黑麦草中添加25%、50%、75%时对DM降解率的影响不显著(P>0.05),添加25%、50%对NDF的降解率影响显著(P<0.05),75%时达到了极显著(P<0.01)。DM、NDF的降解率在添加75%时产生的正效应最高,次之是25%、50%。
苜蓿占25%时对麦秸在12h产气量上的组合效应不显著(P>0.05),50%时显著(P<0.05),75%时达到了极显著(P<0.01);当苜蓿占到25%、50%、75%时,对麦秸在24h、48h的累计产气量和DM、NDF降解率上的组合效应均显著(P<0.05)。麦秸中添加量增大时,12h、24h、48h、DM和NDF降解率的组合效应随之增大,并且在添加75%时达到最大。
玉米秸中添加25%、50%、75%的苜蓿时,在12h累计产气量上的组合效应极显著(P<0.01),24h时添加25%、50%的苜蓿的组合效应极显著(P<0.01),添加75%时显著(P<0.05);48h时添加25%的苜蓿的组合效应极显著(P<0.01),50%、75%时显著(P<0.05);添加25%、50%、75%的苜蓿对玉米秸DM、NDF降解率的影响显著(P<0.05)。苜蓿占25%对12h、24h、48h产气量的组合效应最高,75%时最低;以DM、NDF降解
The study was the determination of in vitro fermentation and gas production characteristics of 5 forages.And influence of in vitro fermentation and gas production characteristics when alfalfa addition to ryegrass,wheatstraw,cornstalk,cornsilage. The results of experiment showed that 5 sorts of roughages affected gas production at 12h,24h,48h,gas permeters,a,b,a+b,c,degradability of DM, NDF at incubated time 48h significantly(P<0.01).And alflafa’s degradability of DM was
higherthanryegrass,cornsilage,cornstalk,wheatstraw.alfalfa’sdegradability of NDF was higherthanryegrass,cornsilage,cornstalk,wheatstraw.5 forges significantly affected ruminal pH,NH_3-N at the end of 48h(p<0.01).
Addition of alflafa significantly affected gas productin at 12h,24h,48h and gas permeters a,b,a+b,candDM,NDFdegradability among forage(P<0.01).DM,NDF degradability increased with the addition of alfalfa,and was higher than no addition the addition of alfalfa to ryegrass significiantly affected combinational effect of gas production at 12h,48h.For 24h,addition 25%,50% of combinational effect wasgreater(P<0.05),but 75% was no significiant. Combinatinal effect for gas was increased with addition of alfalfa.At the hand of DM degrdability,the addition of alfalfa was no significiant,but for NDF degradabiltiy,addition of 25%,50% was significiant,and 75% was greater.For DM,NDF degradability ,75% was higher than 25%,50%.The addition of 25% alfalfa to wheatstraw was no different gas production but 50% was significiant(P<0.05),75% was greater(P<0.01) at 12h. gas at 24h,48h,and DM,NDF degradability were significiant with the addition of alfalfa to wheatstraw (P<0.05).Combinational effect for gas,DM or NDF degradability was increased with addition,and 75% was highest.
The addition of alfalfa to cornstalk significiantly affected combinational effect of gas
黒麦草、麦秸、玉米秸、青贮玉米秸中添加0%、25%、50%、75%、100%的苜蓿在12h、24h、48h的累计产气量及其估测产气参数值a、b、a+b、c、DM和NDF降解率之间差异极显著(P<0.01),DM、NDF降解率随苜蓿添加量的增大而增大,并且添加苜蓿的DM、NDF降解率均高于未添加时的降解率。
黑麦草中苜蓿占到25%、50%、75%时,在12h、48h的累计产气量上的组合效应均显著(P<0.05),对于24h的产气量的组合效应,苜蓿添加25%、50%都达到了显著(P<0.05),但添加75%时不显著(P>0.05),产气效应随着添加量的增加正效应降低;黑麦草中添加25%、50%、75%时对DM降解率的影响不显著(P>0.05),添加25%、50%对NDF的降解率影响显著(P<0.05),75%时达到了极显著(P<0.01)。DM、NDF的降解率在添加75%时产生的正效应最高,次之是25%、50%。
苜蓿占25%时对麦秸在12h产气量上的组合效应不显著(P>0.05),50%时显著(P<0.05),75%时达到了极显著(P<0.01);当苜蓿占到25%、50%、75%时,对麦秸在24h、48h的累计产气量和DM、NDF降解率上的组合效应均显著(P<0.05)。麦秸中添加量增大时,12h、24h、48h、DM和NDF降解率的组合效应随之增大,并且在添加75%时达到最大。
玉米秸中添加25%、50%、75%的苜蓿时,在12h累计产气量上的组合效应极显著(P<0.01),24h时添加25%、50%的苜蓿的组合效应极显著(P<0.01),添加75%时显著(P<0.05);48h时添加25%的苜蓿的组合效应极显著(P<0.01),50%、75%时显著(P<0.05);添加25%、50%、75%的苜蓿对玉米秸DM、NDF降解率的影响显著(P<0.05)。苜蓿占25%对12h、24h、48h产气量的组合效应最高,75%时最低;以DM、NDF降解
The study was the determination of in vitro fermentation and gas production characteristics of 5 forages.And influence of in vitro fermentation and gas production characteristics when alfalfa addition to ryegrass,wheatstraw,cornstalk,cornsilage. The results of experiment showed that 5 sorts of roughages affected gas production at 12h,24h,48h,gas permeters,a,b,a+b,c,degradability of DM, NDF at incubated time 48h significantly(P<0.01).And alflafa’s degradability of DM was
higherthanryegrass,cornsilage,cornstalk,wheatstraw.alfalfa’sdegradability of NDF was higherthanryegrass,cornsilage,cornstalk,wheatstraw.5 forges significantly affected ruminal pH,NH_3-N at the end of 48h(p<0.01).
Addition of alflafa significantly affected gas productin at 12h,24h,48h and gas permeters a,b,a+b,candDM,NDFdegradability among forage(P<0.01).DM,NDF degradability increased with the addition of alfalfa,and was higher than no addition the addition of alfalfa to ryegrass significiantly affected combinational effect of gas production at 12h,48h.For 24h,addition 25%,50% of combinational effect wasgreater(P<0.05),but 75% was no significiant. Combinatinal effect for gas was increased with addition of alfalfa.At the hand of DM degrdability,the addition of alfalfa was no significiant,but for NDF degradabiltiy,addition of 25%,50% was significiant,and 75% was greater.For DM,NDF degradability ,75% was higher than 25%,50%.The addition of 25% alfalfa to wheatstraw was no different gas production but 50% was significiant(P<0.05),75% was greater(P<0.01) at 12h. gas at 24h,48h,and DM,NDF degradability were significiant with the addition of alfalfa to wheatstraw (P<0.05).Combinational effect for gas,DM or NDF degradability was increased with addition,and 75% was highest.
The addition of alfalfa to cornstalk significiantly affected combinational effect of gas
引文
[1]吕小荣,努尔夏提·朱马酉,吕小莲.我国秸秆还田技术现状与发展前景[J].现代化农业,2004,9:41～42.
[2]王晶,周禾.提高秸秆类饲料利用率,大力发展节粮型畜牧业[J].草食家畜,2004,12(4):46～49.
[3]韩正康,陈杰编著.反刍动物瘤胃的消化和代谢[M].科学出版社,1988.
[4]杨再强,王立新,胡建平.攀西河谷退耕还草中四种优质牧草饲用价值的研究[J].青海农林科技,2005,1:3～5.
[5]刘小飞,李科云.黑麦草对奶牛生产性能影响的研究进展[J].饲料与畜牧,2005,4:19～20.
[6]王继强,张波,刘福柱.苜蓿及其产品的营养价值和在畜牧业中的应用[J].饲料广角,2003,16:19～21.
[7]雒秋江.粗饲料的加工和利用[J].新疆畜牧业,1998,3:14～17.
[8]G A Broderick.Performance of lactating dairy cows fed either alfalfa silage or alfalfa hay as the sole forage[J].Journal of Dairy Science,1995,78:321～329.
[9]朱顺国,邢壮,张微,等.玉米秸秆 NDF 与 ADF 含量变化规律的研究[J].中国奶牛,2001,1:24～26.
[10]冯仰廉,张子仪.地质粗饲料的营养价值及合理利用[J].中国畜牧杂志,2001,37(6):3～5.
[11]谷泽涵,刁其玉,张乃锋.粗纤维及其利用中的调控[J].饲料广角,2004,15:14～16.
[12]梁业森.非常规饲料资源的开发与利用[M].北京:中国农业出版社,1996.
[13]彭健.日粮纤维定义、成分、分析方法及加工影响[J].国外畜牧学猪与禽,1999,(4):8～11.
[14]阎贵龙.影响秸秆营养价值的作物学因素及复合化学处理的效果研究[M].中国农业大学,2005,5.
[15]D C Tomlin,R R Johnson,B A Dehority.Relationship of lignification to in vitro cellulose digestibility of grasses and legumes[J].J Anim Sci,1964,23:l 61～161.
[16]E B Cowling,W Brown.Structural features of cellulosic materials in relation to enzymatic hydrolysis.In celluloses and their applications.Hajny GJ and Reese ET[J].Advanced Chemical Series,1969,95:152～187.
[17]R C Campling,M Freer,C C Balch.Factors affecting the voluntary intake of food by cows.The effect of urea on the voluntary intake of oat straw[J].Br.J Nutr,1962,16:115～124.
[18]G P Singly,T J Klopfenstein.Kinetics of microbial protein production and in vitro digestibility of straw ammoniated and/or supplemented with NPN and protein[J].Indian Journal of Animal Sciences,1994,64(8):867～871.
[19]刘建新,吴耀明,叶均安,等.我国南方提高秸秆饲料利用效率的模式研究[M].动物营养研究进展,北京:中国农业大学出版社,1997,274～283.
[20]M W Bosch,S J Tamminga,Van Bruchem.Dietary and animal factor affecting rumen capacity in dairy cows[J].Archives of Animal Breeding,1991,34:469～481.
[21]张吉鹃,李龙瑞,邹庆华.粗饲料在反刍动物营养中的作用及影响粗饲料营养价值的因素[J].畜牧兽医科技信息,2003,19(11):15～18.
[22]王福兆.乳牛学[M].北京:科学技术文献出版社,2004.
[23]D R Mertens.Creating a system for meeting the fiber requirements of dairy cows[J].J Dairy Sci,1997,80:1463～1481.
[24]C B Bailey,C C Balch.Saliva secretion and its relation to feeding in cattle.The composition and rate of secretion in the cow during rest[J].Br 1961,15:371～382
[25]S Michael,Allen.Relationship Between Fermentation Acid Production in the Rumen and the Requirement for Physically Effective Fiber[J].J Dairy Sci,1997,80:1447～1462.
[26]张显东.不同种类粗饲料及添加缓冲剂对绵羊瘤胃发酵的影响[D].东北农业大学,2004,5.
[27]G Chen,M J Wolin.Effect of monensin and lasalocidsodiu on the growth of methanogenic and rumen saccharolytic bacteria[J].Applied andEnvironmental Microbiology,1982,9:253～320.
[28]I D Girard,C R Jones,K A Dawson.Lactic acid utilization rumen-simulating cultures receiving a yeastculture supplement[J].J Anim Sci,1993,71:288～289.
[29]A Bonhomme.Rumen ciliates: their metabolism and relationships with bacteria and their hosts[J].Anim Feed Sci Techno1,1990,30:203～266.
[30]D E Akin,et al.,.Rumen bacterial and fungal degradation of Digitaria pentzii grown with or without sulfur[J].Appl Envir Microbial,1983 46:738～748.
[31]Y W Ho,N Abdullah,S Jalaudin.Colonization of guinea grass by anaerobic fungi in swamp buffalo and cattle[J].Anim Feed Sci Technol,1988,24:161～171.
[32]T T Teeri . Crystalline cellulose degradation:new insight into the function of cellobiohydrolases[J].TrendsBiotechno1,1997,15:160～167.
[33]敬红文.秸秆饲料的加工与利用[J].草食家畜,1999,2:22～24.
[34]李飞,李胜利.生物处理对秸秆的瘤胃干物质降解率的影响[J].中国草食动物,2000,21:3～5.
[35]刘稼芳,李杰.秸秆资源作为饲料的开发利用[J].饲料博览,1998,10(2):20～21.
[36]刘建胜.我国秸秆资源分布及利用现状的分析[D].中国农业大学,2005,2.
[37]张颖,黄必志,毛华明.粗饲料在反刍动物中的营养作用及其影响因素[J].中国动物保健,2005,6:31～33.
[38]晏向华,瞿明仁,黎观红,等.秸秆饲料资源开发技术与应用研究新进展[J].江西饲料,2001,2:19～22.
[39]郭庭双.秸秆畜牧业[M].上海:上海科学技术出版社,1994,21～29.
[40]王钟建.提高秸秆营养价值研究进展[[J].饲料工业,1998,19(5):12～14.
[41]王磊,吕耀奎,高立贞,等.饲料资源开发新技术—热喷[J].江西饲料,1995,(1):18～19.
[42]王学玲,周广森,张美婷,等.热喷氨化锯末饲喂肉牛试验研究「J].中国饲料,1995,(10):30～31.
[43]史兴乔,边革,刘玉英.秸秆饲料的调制技术[J].辽宁畜牧兽医,1998,(5):30～31.
[44]刘学剑.粗饲料的膨化、热喷和微生物发酵技术[J].粮食与饲料工业,1995,(3):22～23.
[45]夏兆刚,孟庆翔.提高秸秆饲用价值的方法和途径[J].饲料研究,1999,6:21～21.
[46]贺鸣.TMR 中粗饲料不同颗粒大小对干奶牛咀嚼行为和瘤胃发酵的影响[D].中国农业大学,2005,6.
[47]A T Siliva,E R ?rskov.Fibre degradation in the rumen of animal receiving hay untreated or ammonia-treated straw[J].Animal Feed Sci and Technology,1988,19:277～288.
[48]S G Haddad,R J Grant,T J Klopfenstein.Digestibility of Alkali-Treated Wheat Straw Measured In Vitro or In Vivo Using Holstein Heifers[J].J Anim Sci.1995,73:3258～3265.
[49]刘德先译.干湿两种方法处理秸秆的效果[J].饲料博览,1986,1:17～17.
[50]Valizadeh R.Study on chemical treatment of wheatstraw with Norea alkali[J].J Proceedings the British Society of Animal Science,2003,168:189～196.
[51]张巧娥,冯家保,王京.氨化稻草饲喂泌乳母牛的试验[J].宁夏农学院学报,1995,16(3):36～38.
[52]朱秋华,钱国英.氨化稻草与羊草饲喂奶牛对比试验[J].上海畜牧兽医通讯,2001,(1):11～13.
[53]许丽,韩友文,张淑芳.不同处理方法对玉米秸不同处理方法对玉米秸干物质和粗纤维瘤胃降解率的影响[J].黑龙江畜牧兽医.2002,5:15～16..
[54]梁刑文主编.饲料原料与品质检测[M].北京:中国林业出版社,1996.
[55]M G Cameron,G C Jr Fahey,J Clark et al.,.Effects of feeding alkaline hydrogen peroxide-treated wheat straw-based diets on intake,digestion,ruminal fermentation,and production responses by mid-lactation dairy cows[J].Anim Sci,1991,69:1775～1787.
[56]张力军,马野,宋显城,等.氨化玉米秸秆饲喂高产奶牛试验[J].饲料博览,1994,(6):10～11.
[57]V Heidarian,A A Naserian,R Valisadeh.The effect of corn silage treated with urea and ammonia on milk production and composition in early lactation Holstein-Friesian cows[J].J Proceedings of the British Society of Animal Science,2004,174:156～163.
[58]A Davtalabzarghi,R Valizadeh,A A Nasserian.The effect.of corn silage treatedwith urea and ammonia on performance of Holstein dairy cows in mid lactation[J].J Proceedings of the British Society of Animal Science,2004,168:152～163.
[59]W F Brown,M B Adjei.Urea ammoniation effects on the feeding value of guineagrass(Panicum maximum)hay[J].J Anim Sci,1995,73:3085～3093.
[60]毛华明,冯仰廉.尿素和氢氧化钙处理作物秸秆提高营养价值的研究[J].中国畜牧杂志,1991,27(5):3～5.
[61]刘伟摘译.粗饲料的氨化[J].饲料广角,1994,6:12～12.
[62]M J Traxler,D G Fox,P J VanSoest et al.,.Predicting forage indigestible NDF from lignin concentration[J].J Anim Sci,1998, 76:1469～1480.
[63]L M Johnson,J H Harrison,D Davidson,et al.,.Corn silage management.Effects of hybrid,maturity, and mechanical processing on chemical and physical characteristics[J].J Dairy Sci,2002,85:833～853.
[64]L M Johnson,J H Harrison,D Davidson,et al.,.Corn Silage Management.Effects of Hybrid,Maturity, Chop Length,and Mechanical Processing on Rate and Extent of Digestion[J].J Dairy Sci,2003,86:3299～3310.
[65]石传林,蒋玉国,范光勇.啤酒糟王米秸混贮料饲喂泌乳牛的效果[J].中国乳业,2001,(11):18～19.
[66]单淑兰,赵友库,王淑荣,等.窝瓜瓢与玉米秸混贮方法和饲喂奶牛效果[J].中国奶牛,1998,(1):30～31.
[65]张为鹏,胡昌军,沈美清.苹果渣与玉米秸混合青贮饲喂奶牛的试验[J].山东畜牧兽医,2002,(6):4～5.
[68]朱国生,李中利.添加纤维素酶制作玉米秸青贮料饲喂奶山羊的试验效果[J].饲料博览,2002,9:42～42.
[69]祁凤华,徐春生,王新峰.多糖降解酶对苜蓿干草营养物质瘤胃降解率的影响[J].石河子大学自然科学(版),2005,23(5):609～612.
[70]李海英,王新峰,徐春生,等.添加复合纤维素酶对粗饲料人工瘤胃产气量和干物质消失率的影响[J].黑龙江畜牧兽医,2006,3:56～59.
[71]孔祥浩.泌乳早期乳牛日粮中添加纤维分解酶的效果[J].饲料博览,2000,6:26～28.
[72]K A Beauchemin,L M Rode,et al.,.Fibrolytic enzymes increase fiber digestibility and growth rate of steers fed dry forages[J].Can J Anim Sci,1995,75:641～644.
[73]G E Lewis.effect of direct fed fibrolytic enzymes on the digestive characteristics of a forage based diet fed to beef steers[J].J Anim Sci,1996,74:3020～3028.
[74]D J Schingoethe.Response of lactating dairy cows to a cellulase and xylanase enzyme mixture applied to forages at the time of feeding[J].J Dairy Sci,1999,82(5):996～1003.
[75]L Kung.The effect of treating forages with fibrolytic enzymes on its nutritive value and lactation performance on of dairy cows[J].Journal of Dairy Science,2000,83:112～115.
[76]H Fazaeli,A Azizi,Z A M Jelan et al.,.Effect of fungal treatmenton the chemical composition,in vitro digestibility and in sacco degradability of wheat straw[J].Proceedings of the British Society of Animal Science,2003,14:125～134.
[77]Y Wang,B M Spratling,D R ZoBell et al.,.Effect of alkali pretreatment of wheat straw on the effcacy of exogenous fibrolytic enzymes[J].J Anim Sci,2004,82:198～208.
[78]李秀波.有效微生物菌群及其在养殖业中的应用[J].国外畜牧科技,1997,24(5):24～26.
[79]李义海,谢宏.活菌制剂在反刍家畜饲养中的应用[J].饲料工业,1998,19(1) :30～3138.
[80]刘晓强.菌化秸秆饲料研究[J].草食家畜,1998,4:39～40.
[81]孟庆翔,肖训军,俞宏,等.微生物处理小麦秸作为牛长肥育牛饲料的营养价值[J].中国畜牧杂志,1999,35(6):3～5.
[82]涂国全,张宏玉,张宝,等.EM 在粗词料发酵中的转化效果[J].中国饲料,1999,16:9～11.
[83]王建兵,韩继福,高宏伟,等.微生物接种剂和酶制剂对玉米秸秆发酵品质的影响[J].内蒙古畜牧科学.2001,2:17～19
[84]郭旭生,崔慰贤.提高秸秆饲料利用率和营养价值的研究进展[J].宁夏农学院学报,2002,23(3):56～60。
[85]潭支良,卢德勋.提高粗饲料利用效率的系统组合营养技术及其组合效应的研究进展[J].饲料博览,1999,11(7):6～10.
[86]朱宇涟,张勇.反刍动物粗饲料利用的营养调控[J].草业科学,2003 ,20(6):636～39.
[87]F L Mould, E R Φrskov, S O Mann.Associative effects of mixed feeds.1.Effects of type and level of supplementation and the influence of the rumen fluid pH on cellulolysis in vivo and dry matter digestion of various roughages[J].Animal Feed Science and Technology, 1983, 10:15～30.
[88]G Y Zhao,P Lebzien.Developent of an in vitro incubation technique for the estimation of the utilizable crude protein (uCP)in feeds for cattle[J].Arch Anim Nutri,2000,153:293～302.
[89]A K Mosi,M H Butterworth.The voluntary intake and digestibility of combination of cerea lcrep residue and legume hay for sheep[J].Animal Feed Science and Technoligy,1995,12:241～251.
[90]A bdulrazak.Supplementation with Gliricidia sepium and leucaenaeu cocephala on voluntary food intake,digestibility,rumen fermentation and liveweigh to fcross bred steers offered Zeamays stover[J].Livestock Production Science,1997,49:53～62.
[91]苏海涯.反刍动物饲料日粮中桑叶与饼粕类饲料间组合效应的研究[D].浙江大学.2002,5.
[92]周传社,汤少勋,姜海林,等.农作物秸秆体外发酵营养特性及其组合利用研究[J].应用生态学报,2005,10(16):1862～1867.
[93]郭冬生.反刍动物日粮组合效应对瘤胃发酵和可利用粗蛋白的影响研究[D].中国农业大学,2004,6.
[94]J M A Tilley,R A Terry.A two stage technique for the in-vitro digestion of forage crops[J].J Brit Grassl Soc,1963,18:104～111.
[95]K H Menke,A Salewski,H Steingass et al.,.The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when liquor in vitro[J].J Agr Sci,1979,93:217～222.
[96]U Krirhnamoorthy,H Soller,H Steingass et al.,.Comparative study on rumen fermentation of energy supplements in vitro[J].J Anim Physiollgy and Anim Nutrition,1991,65:20～35.
[97]P H Doane,P Schofield,A N Pell.Neutral Detergent Fiber Disappearance and Gas and Volatile Fatty Acid;Production During the In Vitro Fermentation of Six Forages[J].J Anim Sci,1997,75:3342～3352.
[98]孙世民.精料型日粮中添加抗生素对绵羊消化代谢的影响[D].新疆农业大学,2004,6.
[99]K Menke,L Raab,A Salewski,et al.,.The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production technique when they are incubated with rumen liquor in vitro[J].J Agric Sci Cambridge ,1979,93:217～222.
[100]丁角立,张维汉.用活体外人工瘤胃法评定反刍动物饲料能量价值和有机物消化率[J].中国畜牧杂志,1983,5:2～5.
[101]金得哲,王苏宁.应用人工瘤胃法评定梅花鹿常用饲料营养价值的研究[J].中国畜牧杂志,1987,6:7～10.
[102]陈化鹏,李枫,孙中武,等.体外消化试验法的初步评价[J].东北林业大学学报,1997,25(5):81～84.
[103]J A Huntington,C Rymer,D I Givers.The effect of host diet on the gas production of hay and high-temperature dried grass[J].Animal Science,1998, 67(1):59～64.
[104]J Scehovic.In vitro measurement of rumen microbial activity in the presence of extracts from natural grassland spieces[J].Forages,1998, 154:249～260.
[105]G P Singh,Madhu Mohini.Level of green maize affecting methane production on roughage based diet[J].Indian Journal of Animal Sciences,1999,69(1):54～58.
[106]A A M Marinho.Modifications in the reticulum-rumen of sheep fed high nitrate diets.Influence of nitrite concentration on cellulose fermentation in vitro[J].Portuguesa de Ciências Veterinárias,1999,94(532):189～193.
[107]Z S Davies,D Mason,A E BrooKs et al.,.An automated system for measuring gas production from forages inoculated with rumen fluid and its use determining the effect of enzymes on grass silage[J].Animal Feed Science and Technology,2000,83(3/4):205～210.
[108]赵广永,冯仰廉.用短期人工瘤胃发酵法研究饮水温度对反刍动物瘤胃发酵的影响[J].中国畜牧杂志,1996,32(5):8～10.
[109]赵广永,冯仰廉.皂化菜子油脚对人工瘤胃发酵的影响[J].中国畜牧杂志,1999,35(1):10～11.
[110]孟庆祥.精料水平与秸秆氨化对绵羊日粮消化、氮存留与进食量的影响[J].北京农业大学学报,1991,(3):109～111.
[111]K P Aiple,H Steingass,W Drochner.Prediction of the net energy content of raw materials and compound feeds for ruminants by different laboratory methods[J].Arch Anim Nutr,1996,49:213～220.
[112]吴天星,彭大惠.应用羊粪液取代瘤胃液两级离体消化试验的探索[J].动物营养学报,1995,7(4):33～37.
[113]王全军,毛胜勇.水牛瘤胃液和粪便中微生物区系体外发酵活力的比较[J].中国草食动物,2000,2(3):10～11.
[114]张永根,王志博,宋 平,等.用粪液法与尼龙袋法测定牧草有机物和蛋白质降解率的比较研究[J].东北农业大学学报,2005,36(6):750～755.
[115]卢德勋,谢崇文.现代反刍动物营养研究方法和技术[M].农业出版社:1991.
[116]Lu D-X(卢德勋),Xie C-W(谢崇文).The Research Methods and Techniques on Ruminant Nutrition[M].Beijing:Agriculture Press,1990.
[117]杨胜.饲料分析及饲料质量检测技术[M].北京:中国农业出版社,1994.
[118]许琴.利用体外产气法研究玉米秸秆瘤胃发酵的影响因素[D].新疆农业大学,2002,5.
[119]E R Φrskov,M c Donald L.The estimation of protein degradability in the rumen from incubation measurements weighted according to the rate of passage[J].Journal of Agricultural Science Cambridge,1979,92:499～503.
[120]胡小平,王长发.SAS 基础及统计实例教程[M].西安地图出版社.2001,7.
[121]张勇.反刍动物粗饲料利用的营养调控[J].草业科学,2003,20(6):36～39.
[122]P C Hoff man,D K combs et al.,.Performance of lactating dairy cows fed Alfalfa silage or perennial ryegrass silage[J].Journal of Dairy Science,1998,81(1):162～168.
[123]G Getachew,P H Robinson et al.,.Relation between chemical composition,dry matter degradation and in vitro gas production of several ruminant feeds[J].J Animal Sci,2004,111:57～71.
[124]王加启,冯仰廉.不同粗饲料日粮发酵规律及合成瘤胃微生物蛋白质效率研究[J].黄牛杂志,1994,20:82～84.
[125]高腾云,王艳玲,郭金玲,等.粗饲料及肉牛日粮的降解规律研究[J].黄牛杂志,1998,24(5):11～13.
[126]刘大林,赵国琦,王学峰,等.豆科与禾本科牧草在山羊瘤胃内的降解率的比较[J].四川畜牧兽医,2000,(3):9～11.
[127]王加启,冯仰廉.日粮精粗比对瘤胃微生物合成效率的影响[J].畜牧兽医学报,1995,26(4):301～307.
[128]王云.绵羊日粮中添加青绿饲料对饲料营养成分瘤胃降解率的影响[D].山东农业大学,2003,6.
[129]刘圈炜,何云,王成章,等.苜蓿在反刍动物饲料中应用的研究进展[J].粮食与饲料工业,2006,3:30～31.
[130]郭艳丽.含高果胶饲料及不同加工处理的饲粮对绵羊生产性能、瘤胃和血液代谢参数的影响[D].2004,6.
[131]李莉.体外法研究肤对瘤胃液pH、氨氮浓度、菌体蛋白氮浓度、中性洗涤纤维降解率及其产气量的影响[D].内蒙古农业大学,2001,6.
[132]郭旭生.秸秆生化复合处理技术及系统营养调控在肉羊日粮配制中的应用研究[D].宁夏大学,2004,4.
[133]J X Liu,A Susenbeth,K H Sudekum.In vitro gas production measurements to evaluate interactions between untreated and chemically treated ricestraws,grass hay,and mulberry leaves[J].J Anim Sci,2002,80:517～524.
[134]F L Mould,E R ?rskove.Manipulation of rumen fluid pH and its influence on cellulolysis in sacco, dry matter degradation and the rumen microflora of sheep offered either hay or concentrate[J].Anim Feed Sci Technol,1983,10:1～4.
[135]W H Hoover,S R Storkes.Balancing carbohydrates and proteins for optimum rumen microbial yield[J].J DairySci,1991,74:36～30.
[136]M F J Van Houtert.The production and metabolism of volatile fatty acids by ruminants fed roughages:A review[J].Anim Feed Sci Technol,1993,43:189～225.
[137]孙国君,潘晓亮,祁凤华等.粗饲料氨基酸在瘤胃内降解规律研究[J].中国饲料,2003,15:11～12.
[138]刘哲,张昌吉,郝正里等.饲喂含不同秸秆的全日粮颗粒料对绵羊瘤胃及血液代谢参数的影响[J].中国饲料,2005,11:12～14.
[139]刘哲.含不同秸秆的全日粮颗粒料对绵羊瘤胃代谢参数及营养物质消化代谢的影响[D].甘肃农业大学,2004,6.
[2]王晶,周禾.提高秸秆类饲料利用率,大力发展节粮型畜牧业[J].草食家畜,2004,12(4):46～49.
[3]韩正康,陈杰编著.反刍动物瘤胃的消化和代谢[M].科学出版社,1988.
[4]杨再强,王立新,胡建平.攀西河谷退耕还草中四种优质牧草饲用价值的研究[J].青海农林科技,2005,1:3～5.
[5]刘小飞,李科云.黑麦草对奶牛生产性能影响的研究进展[J].饲料与畜牧,2005,4:19～20.
[6]王继强,张波,刘福柱.苜蓿及其产品的营养价值和在畜牧业中的应用[J].饲料广角,2003,16:19～21.
[7]雒秋江.粗饲料的加工和利用[J].新疆畜牧业,1998,3:14～17.
[8]G A Broderick.Performance of lactating dairy cows fed either alfalfa silage or alfalfa hay as the sole forage[J].Journal of Dairy Science,1995,78:321～329.
[9]朱顺国,邢壮,张微,等.玉米秸秆 NDF 与 ADF 含量变化规律的研究[J].中国奶牛,2001,1:24～26.
[10]冯仰廉,张子仪.地质粗饲料的营养价值及合理利用[J].中国畜牧杂志,2001,37(6):3～5.
[11]谷泽涵,刁其玉,张乃锋.粗纤维及其利用中的调控[J].饲料广角,2004,15:14～16.
[12]梁业森.非常规饲料资源的开发与利用[M].北京:中国农业出版社,1996.
[13]彭健.日粮纤维定义、成分、分析方法及加工影响[J].国外畜牧学猪与禽,1999,(4):8～11.
[14]阎贵龙.影响秸秆营养价值的作物学因素及复合化学处理的效果研究[M].中国农业大学,2005,5.
[15]D C Tomlin,R R Johnson,B A Dehority.Relationship of lignification to in vitro cellulose digestibility of grasses and legumes[J].J Anim Sci,1964,23:l 61～161.
[16]E B Cowling,W Brown.Structural features of cellulosic materials in relation to enzymatic hydrolysis.In celluloses and their applications.Hajny GJ and Reese ET[J].Advanced Chemical Series,1969,95:152～187.
[17]R C Campling,M Freer,C C Balch.Factors affecting the voluntary intake of food by cows.The effect of urea on the voluntary intake of oat straw[J].Br.J Nutr,1962,16:115～124.
[18]G P Singly,T J Klopfenstein.Kinetics of microbial protein production and in vitro digestibility of straw ammoniated and/or supplemented with NPN and protein[J].Indian Journal of Animal Sciences,1994,64(8):867～871.
[19]刘建新,吴耀明,叶均安,等.我国南方提高秸秆饲料利用效率的模式研究[M].动物营养研究进展,北京:中国农业大学出版社,1997,274～283.
[20]M W Bosch,S J Tamminga,Van Bruchem.Dietary and animal factor affecting rumen capacity in dairy cows[J].Archives of Animal Breeding,1991,34:469～481.
[21]张吉鹃,李龙瑞,邹庆华.粗饲料在反刍动物营养中的作用及影响粗饲料营养价值的因素[J].畜牧兽医科技信息,2003,19(11):15～18.
[22]王福兆.乳牛学[M].北京:科学技术文献出版社,2004.
[23]D R Mertens.Creating a system for meeting the fiber requirements of dairy cows[J].J Dairy Sci,1997,80:1463～1481.
[24]C B Bailey,C C Balch.Saliva secretion and its relation to feeding in cattle.The composition and rate of secretion in the cow during rest[J].Br 1961,15:371～382
[25]S Michael,Allen.Relationship Between Fermentation Acid Production in the Rumen and the Requirement for Physically Effective Fiber[J].J Dairy Sci,1997,80:1447～1462.
[26]张显东.不同种类粗饲料及添加缓冲剂对绵羊瘤胃发酵的影响[D].东北农业大学,2004,5.
[27]G Chen,M J Wolin.Effect of monensin and lasalocidsodiu on the growth of methanogenic and rumen saccharolytic bacteria[J].Applied andEnvironmental Microbiology,1982,9:253～320.
[28]I D Girard,C R Jones,K A Dawson.Lactic acid utilization rumen-simulating cultures receiving a yeastculture supplement[J].J Anim Sci,1993,71:288～289.
[29]A Bonhomme.Rumen ciliates: their metabolism and relationships with bacteria and their hosts[J].Anim Feed Sci Techno1,1990,30:203～266.
[30]D E Akin,et al.,.Rumen bacterial and fungal degradation of Digitaria pentzii grown with or without sulfur[J].Appl Envir Microbial,1983 46:738～748.
[31]Y W Ho,N Abdullah,S Jalaudin.Colonization of guinea grass by anaerobic fungi in swamp buffalo and cattle[J].Anim Feed Sci Technol,1988,24:161～171.
[32]T T Teeri . Crystalline cellulose degradation:new insight into the function of cellobiohydrolases[J].TrendsBiotechno1,1997,15:160～167.
[33]敬红文.秸秆饲料的加工与利用[J].草食家畜,1999,2:22～24.
[34]李飞,李胜利.生物处理对秸秆的瘤胃干物质降解率的影响[J].中国草食动物,2000,21:3～5.
[35]刘稼芳,李杰.秸秆资源作为饲料的开发利用[J].饲料博览,1998,10(2):20～21.
[36]刘建胜.我国秸秆资源分布及利用现状的分析[D].中国农业大学,2005,2.
[37]张颖,黄必志,毛华明.粗饲料在反刍动物中的营养作用及其影响因素[J].中国动物保健,2005,6:31～33.
[38]晏向华,瞿明仁,黎观红,等.秸秆饲料资源开发技术与应用研究新进展[J].江西饲料,2001,2:19～22.
[39]郭庭双.秸秆畜牧业[M].上海:上海科学技术出版社,1994,21～29.
[40]王钟建.提高秸秆营养价值研究进展[[J].饲料工业,1998,19(5):12～14.
[41]王磊,吕耀奎,高立贞,等.饲料资源开发新技术—热喷[J].江西饲料,1995,(1):18～19.
[42]王学玲,周广森,张美婷,等.热喷氨化锯末饲喂肉牛试验研究「J].中国饲料,1995,(10):30～31.
[43]史兴乔,边革,刘玉英.秸秆饲料的调制技术[J].辽宁畜牧兽医,1998,(5):30～31.
[44]刘学剑.粗饲料的膨化、热喷和微生物发酵技术[J].粮食与饲料工业,1995,(3):22～23.
[45]夏兆刚,孟庆翔.提高秸秆饲用价值的方法和途径[J].饲料研究,1999,6:21～21.
[46]贺鸣.TMR 中粗饲料不同颗粒大小对干奶牛咀嚼行为和瘤胃发酵的影响[D].中国农业大学,2005,6.
[47]A T Siliva,E R ?rskov.Fibre degradation in the rumen of animal receiving hay untreated or ammonia-treated straw[J].Animal Feed Sci and Technology,1988,19:277～288.
[48]S G Haddad,R J Grant,T J Klopfenstein.Digestibility of Alkali-Treated Wheat Straw Measured In Vitro or In Vivo Using Holstein Heifers[J].J Anim Sci.1995,73:3258～3265.
[49]刘德先译.干湿两种方法处理秸秆的效果[J].饲料博览,1986,1:17～17.
[50]Valizadeh R.Study on chemical treatment of wheatstraw with Norea alkali[J].J Proceedings the British Society of Animal Science,2003,168:189～196.
[51]张巧娥,冯家保,王京.氨化稻草饲喂泌乳母牛的试验[J].宁夏农学院学报,1995,16(3):36～38.
[52]朱秋华,钱国英.氨化稻草与羊草饲喂奶牛对比试验[J].上海畜牧兽医通讯,2001,(1):11～13.
[53]许丽,韩友文,张淑芳.不同处理方法对玉米秸不同处理方法对玉米秸干物质和粗纤维瘤胃降解率的影响[J].黑龙江畜牧兽医.2002,5:15～16..
[54]梁刑文主编.饲料原料与品质检测[M].北京:中国林业出版社,1996.
[55]M G Cameron,G C Jr Fahey,J Clark et al.,.Effects of feeding alkaline hydrogen peroxide-treated wheat straw-based diets on intake,digestion,ruminal fermentation,and production responses by mid-lactation dairy cows[J].Anim Sci,1991,69:1775～1787.
[56]张力军,马野,宋显城,等.氨化玉米秸秆饲喂高产奶牛试验[J].饲料博览,1994,(6):10～11.
[57]V Heidarian,A A Naserian,R Valisadeh.The effect of corn silage treated with urea and ammonia on milk production and composition in early lactation Holstein-Friesian cows[J].J Proceedings of the British Society of Animal Science,2004,174:156～163.
[58]A Davtalabzarghi,R Valizadeh,A A Nasserian.The effect.of corn silage treatedwith urea and ammonia on performance of Holstein dairy cows in mid lactation[J].J Proceedings of the British Society of Animal Science,2004,168:152～163.
[59]W F Brown,M B Adjei.Urea ammoniation effects on the feeding value of guineagrass(Panicum maximum)hay[J].J Anim Sci,1995,73:3085～3093.
[60]毛华明,冯仰廉.尿素和氢氧化钙处理作物秸秆提高营养价值的研究[J].中国畜牧杂志,1991,27(5):3～5.
[61]刘伟摘译.粗饲料的氨化[J].饲料广角,1994,6:12～12.
[62]M J Traxler,D G Fox,P J VanSoest et al.,.Predicting forage indigestible NDF from lignin concentration[J].J Anim Sci,1998, 76:1469～1480.
[63]L M Johnson,J H Harrison,D Davidson,et al.,.Corn silage management.Effects of hybrid,maturity, and mechanical processing on chemical and physical characteristics[J].J Dairy Sci,2002,85:833～853.
[64]L M Johnson,J H Harrison,D Davidson,et al.,.Corn Silage Management.Effects of Hybrid,Maturity, Chop Length,and Mechanical Processing on Rate and Extent of Digestion[J].J Dairy Sci,2003,86:3299～3310.
[65]石传林,蒋玉国,范光勇.啤酒糟王米秸混贮料饲喂泌乳牛的效果[J].中国乳业,2001,(11):18～19.
[66]单淑兰,赵友库,王淑荣,等.窝瓜瓢与玉米秸混贮方法和饲喂奶牛效果[J].中国奶牛,1998,(1):30～31.
[65]张为鹏,胡昌军,沈美清.苹果渣与玉米秸混合青贮饲喂奶牛的试验[J].山东畜牧兽医,2002,(6):4～5.
[68]朱国生,李中利.添加纤维素酶制作玉米秸青贮料饲喂奶山羊的试验效果[J].饲料博览,2002,9:42～42.
[69]祁凤华,徐春生,王新峰.多糖降解酶对苜蓿干草营养物质瘤胃降解率的影响[J].石河子大学自然科学(版),2005,23(5):609～612.
[70]李海英,王新峰,徐春生,等.添加复合纤维素酶对粗饲料人工瘤胃产气量和干物质消失率的影响[J].黑龙江畜牧兽医,2006,3:56～59.
[71]孔祥浩.泌乳早期乳牛日粮中添加纤维分解酶的效果[J].饲料博览,2000,6:26～28.
[72]K A Beauchemin,L M Rode,et al.,.Fibrolytic enzymes increase fiber digestibility and growth rate of steers fed dry forages[J].Can J Anim Sci,1995,75:641～644.
[73]G E Lewis.effect of direct fed fibrolytic enzymes on the digestive characteristics of a forage based diet fed to beef steers[J].J Anim Sci,1996,74:3020～3028.
[74]D J Schingoethe.Response of lactating dairy cows to a cellulase and xylanase enzyme mixture applied to forages at the time of feeding[J].J Dairy Sci,1999,82(5):996～1003.
[75]L Kung.The effect of treating forages with fibrolytic enzymes on its nutritive value and lactation performance on of dairy cows[J].Journal of Dairy Science,2000,83:112～115.
[76]H Fazaeli,A Azizi,Z A M Jelan et al.,.Effect of fungal treatmenton the chemical composition,in vitro digestibility and in sacco degradability of wheat straw[J].Proceedings of the British Society of Animal Science,2003,14:125～134.
[77]Y Wang,B M Spratling,D R ZoBell et al.,.Effect of alkali pretreatment of wheat straw on the effcacy of exogenous fibrolytic enzymes[J].J Anim Sci,2004,82:198～208.
[78]李秀波.有效微生物菌群及其在养殖业中的应用[J].国外畜牧科技,1997,24(5):24～26.
[79]李义海,谢宏.活菌制剂在反刍家畜饲养中的应用[J].饲料工业,1998,19(1) :30～3138.
[80]刘晓强.菌化秸秆饲料研究[J].草食家畜,1998,4:39～40.
[81]孟庆翔,肖训军,俞宏,等.微生物处理小麦秸作为牛长肥育牛饲料的营养价值[J].中国畜牧杂志,1999,35(6):3～5.
[82]涂国全,张宏玉,张宝,等.EM 在粗词料发酵中的转化效果[J].中国饲料,1999,16:9～11.
[83]王建兵,韩继福,高宏伟,等.微生物接种剂和酶制剂对玉米秸秆发酵品质的影响[J].内蒙古畜牧科学.2001,2:17～19
[84]郭旭生,崔慰贤.提高秸秆饲料利用率和营养价值的研究进展[J].宁夏农学院学报,2002,23(3):56～60。
[85]潭支良,卢德勋.提高粗饲料利用效率的系统组合营养技术及其组合效应的研究进展[J].饲料博览,1999,11(7):6～10.
[86]朱宇涟,张勇.反刍动物粗饲料利用的营养调控[J].草业科学,2003 ,20(6):636～39.
[87]F L Mould, E R Φrskov, S O Mann.Associative effects of mixed feeds.1.Effects of type and level of supplementation and the influence of the rumen fluid pH on cellulolysis in vivo and dry matter digestion of various roughages[J].Animal Feed Science and Technology, 1983, 10:15～30.
[88]G Y Zhao,P Lebzien.Developent of an in vitro incubation technique for the estimation of the utilizable crude protein (uCP)in feeds for cattle[J].Arch Anim Nutri,2000,153:293～302.
[89]A K Mosi,M H Butterworth.The voluntary intake and digestibility of combination of cerea lcrep residue and legume hay for sheep[J].Animal Feed Science and Technoligy,1995,12:241～251.
[90]A bdulrazak.Supplementation with Gliricidia sepium and leucaenaeu cocephala on voluntary food intake,digestibility,rumen fermentation and liveweigh to fcross bred steers offered Zeamays stover[J].Livestock Production Science,1997,49:53～62.
[91]苏海涯.反刍动物饲料日粮中桑叶与饼粕类饲料间组合效应的研究[D].浙江大学.2002,5.
[92]周传社,汤少勋,姜海林,等.农作物秸秆体外发酵营养特性及其组合利用研究[J].应用生态学报,2005,10(16):1862～1867.
[93]郭冬生.反刍动物日粮组合效应对瘤胃发酵和可利用粗蛋白的影响研究[D].中国农业大学,2004,6.
[94]J M A Tilley,R A Terry.A two stage technique for the in-vitro digestion of forage crops[J].J Brit Grassl Soc,1963,18:104～111.
[95]K H Menke,A Salewski,H Steingass et al.,.The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when liquor in vitro[J].J Agr Sci,1979,93:217～222.
[96]U Krirhnamoorthy,H Soller,H Steingass et al.,.Comparative study on rumen fermentation of energy supplements in vitro[J].J Anim Physiollgy and Anim Nutrition,1991,65:20～35.
[97]P H Doane,P Schofield,A N Pell.Neutral Detergent Fiber Disappearance and Gas and Volatile Fatty Acid;Production During the In Vitro Fermentation of Six Forages[J].J Anim Sci,1997,75:3342～3352.
[98]孙世民.精料型日粮中添加抗生素对绵羊消化代谢的影响[D].新疆农业大学,2004,6.
[99]K Menke,L Raab,A Salewski,et al.,.The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production technique when they are incubated with rumen liquor in vitro[J].J Agric Sci Cambridge ,1979,93:217～222.
[100]丁角立,张维汉.用活体外人工瘤胃法评定反刍动物饲料能量价值和有机物消化率[J].中国畜牧杂志,1983,5:2～5.
[101]金得哲,王苏宁.应用人工瘤胃法评定梅花鹿常用饲料营养价值的研究[J].中国畜牧杂志,1987,6:7～10.
[102]陈化鹏,李枫,孙中武,等.体外消化试验法的初步评价[J].东北林业大学学报,1997,25(5):81～84.
[103]J A Huntington,C Rymer,D I Givers.The effect of host diet on the gas production of hay and high-temperature dried grass[J].Animal Science,1998, 67(1):59～64.
[104]J Scehovic.In vitro measurement of rumen microbial activity in the presence of extracts from natural grassland spieces[J].Forages,1998, 154:249～260.
[105]G P Singh,Madhu Mohini.Level of green maize affecting methane production on roughage based diet[J].Indian Journal of Animal Sciences,1999,69(1):54～58.
[106]A A M Marinho.Modifications in the reticulum-rumen of sheep fed high nitrate diets.Influence of nitrite concentration on cellulose fermentation in vitro[J].Portuguesa de Ciências Veterinárias,1999,94(532):189～193.
[107]Z S Davies,D Mason,A E BrooKs et al.,.An automated system for measuring gas production from forages inoculated with rumen fluid and its use determining the effect of enzymes on grass silage[J].Animal Feed Science and Technology,2000,83(3/4):205～210.
[108]赵广永,冯仰廉.用短期人工瘤胃发酵法研究饮水温度对反刍动物瘤胃发酵的影响[J].中国畜牧杂志,1996,32(5):8～10.
[109]赵广永,冯仰廉.皂化菜子油脚对人工瘤胃发酵的影响[J].中国畜牧杂志,1999,35(1):10～11.
[110]孟庆祥.精料水平与秸秆氨化对绵羊日粮消化、氮存留与进食量的影响[J].北京农业大学学报,1991,(3):109～111.
[111]K P Aiple,H Steingass,W Drochner.Prediction of the net energy content of raw materials and compound feeds for ruminants by different laboratory methods[J].Arch Anim Nutr,1996,49:213～220.
[112]吴天星,彭大惠.应用羊粪液取代瘤胃液两级离体消化试验的探索[J].动物营养学报,1995,7(4):33～37.
[113]王全军,毛胜勇.水牛瘤胃液和粪便中微生物区系体外发酵活力的比较[J].中国草食动物,2000,2(3):10～11.
[114]张永根,王志博,宋 平,等.用粪液法与尼龙袋法测定牧草有机物和蛋白质降解率的比较研究[J].东北农业大学学报,2005,36(6):750～755.
[115]卢德勋,谢崇文.现代反刍动物营养研究方法和技术[M].农业出版社:1991.
[116]Lu D-X(卢德勋),Xie C-W(谢崇文).The Research Methods and Techniques on Ruminant Nutrition[M].Beijing:Agriculture Press,1990.
[117]杨胜.饲料分析及饲料质量检测技术[M].北京:中国农业出版社,1994.
[118]许琴.利用体外产气法研究玉米秸秆瘤胃发酵的影响因素[D].新疆农业大学,2002,5.
[119]E R Φrskov,M c Donald L.The estimation of protein degradability in the rumen from incubation measurements weighted according to the rate of passage[J].Journal of Agricultural Science Cambridge,1979,92:499～503.
[120]胡小平,王长发.SAS 基础及统计实例教程[M].西安地图出版社.2001,7.
[121]张勇.反刍动物粗饲料利用的营养调控[J].草业科学,2003,20(6):36～39.
[122]P C Hoff man,D K combs et al.,.Performance of lactating dairy cows fed Alfalfa silage or perennial ryegrass silage[J].Journal of Dairy Science,1998,81(1):162～168.
[123]G Getachew,P H Robinson et al.,.Relation between chemical composition,dry matter degradation and in vitro gas production of several ruminant feeds[J].J Animal Sci,2004,111:57～71.
[124]王加启,冯仰廉.不同粗饲料日粮发酵规律及合成瘤胃微生物蛋白质效率研究[J].黄牛杂志,1994,20:82～84.
[125]高腾云,王艳玲,郭金玲,等.粗饲料及肉牛日粮的降解规律研究[J].黄牛杂志,1998,24(5):11～13.
[126]刘大林,赵国琦,王学峰,等.豆科与禾本科牧草在山羊瘤胃内的降解率的比较[J].四川畜牧兽医,2000,(3):9～11.
[127]王加启,冯仰廉.日粮精粗比对瘤胃微生物合成效率的影响[J].畜牧兽医学报,1995,26(4):301～307.
[128]王云.绵羊日粮中添加青绿饲料对饲料营养成分瘤胃降解率的影响[D].山东农业大学,2003,6.
[129]刘圈炜,何云,王成章,等.苜蓿在反刍动物饲料中应用的研究进展[J].粮食与饲料工业,2006,3:30～31.
[130]郭艳丽.含高果胶饲料及不同加工处理的饲粮对绵羊生产性能、瘤胃和血液代谢参数的影响[D].2004,6.
[131]李莉.体外法研究肤对瘤胃液pH、氨氮浓度、菌体蛋白氮浓度、中性洗涤纤维降解率及其产气量的影响[D].内蒙古农业大学,2001,6.
[132]郭旭生.秸秆生化复合处理技术及系统营养调控在肉羊日粮配制中的应用研究[D].宁夏大学,2004,4.
[133]J X Liu,A Susenbeth,K H Sudekum.In vitro gas production measurements to evaluate interactions between untreated and chemically treated ricestraws,grass hay,and mulberry leaves[J].J Anim Sci,2002,80:517～524.
[134]F L Mould,E R ?rskove.Manipulation of rumen fluid pH and its influence on cellulolysis in sacco, dry matter degradation and the rumen microflora of sheep offered either hay or concentrate[J].Anim Feed Sci Technol,1983,10:1～4.
[135]W H Hoover,S R Storkes.Balancing carbohydrates and proteins for optimum rumen microbial yield[J].J DairySci,1991,74:36～30.
[136]M F J Van Houtert.The production and metabolism of volatile fatty acids by ruminants fed roughages:A review[J].Anim Feed Sci Technol,1993,43:189～225.
[137]孙国君,潘晓亮,祁凤华等.粗饲料氨基酸在瘤胃内降解规律研究[J].中国饲料,2003,15:11～12.
[138]刘哲,张昌吉,郝正里等.饲喂含不同秸秆的全日粮颗粒料对绵羊瘤胃及血液代谢参数的影响[J].中国饲料,2005,11:12～14.
[139]刘哲.含不同秸秆的全日粮颗粒料对绵羊瘤胃代谢参数及营养物质消化代谢的影响[D].甘肃农业大学,2004,6.