植物乳杆菌转化亚油酸生成共轭亚油酸的研究
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摘要
共轭亚油酸(CLA)有很好的抗癌、减肥、抗动脉硬化等生理活性。目前,CLA工业化生产是由化学异构法制得,其产物中异构体组成复杂,不利于在食品和医药方面使用。亚油酸生物异构化生成CLA,产物中异构体组成单一,乳酸菌异构化亚油酸生成CLA有很好的应用前景。
本课题对Lactobacillus plantarum转化亚油酸生成CLA过程进行了研究,内容包括菌种分离、筛选、鉴定,菌种诱变选育,CLA发酵过程动力学,洗涤细胞转化亚油酸生成CLA,亚油酸异构酶分离纯化,酶学性质研究和提高发酵牛乳CLA含量实验研究。论文主要内容如下:
1) 从酸菜中分离出一株较高CLA产量的乳酸菌L_(29),鉴定为植物乳杆菌(Lactobacillusplanmrum置信度96.1%)。
2) 以L.plantarum L_(29)为出发菌株,通过紫外线照射和硫酸二乙酯(DES)诱变处理,选育出突变株L.plantarum LT2-6,CLA产量提高18.6%。
3) 对L.planmrum LT2-6在5 L罐规模的分批发酵过程进行了实验分析研究,获得了分批发酵过程动力学曲线,建立了分批发酵过程菌体生长、产物CLA生成和底物(葡萄糖)代谢数学模型。
4) Lplanmrum LT2-6洗涤细胞转化亚油酸生成CLA优化条件为:30℃,pH 7.0,亚油酸浓度1.5%(w/v),细胞浓度20%(w/v)。反应64 h,CLA产量达到最大(7.86g/L),亚油酸转化率为52.4%。经脂肪酸萃取和甲酯化,气相色谱检测显示,土要产物为cis-,trans-9,11.CLA。
5) L.plantarum LT2-6亚油酸异构酶分子量约为48.8 KDa,最适pH 6.0,最适温度30℃,不耐热,温度高于50℃时,稳定性下降很快。金属离子对此酶活力影响顺序为:Mg~(2+)>Ca~(2+)>Mn~(2+)>Fe~(2+)>Zn~(2+)>Na~+。酶动力学参数为:K_m=12.46 μmol/L,V_m=1.933×10~(-1)/μmol/h.mg。
6) 以L.plantarum LT2-6为牛乳发酵剂,转化亚油酸生成CLA的适宜温度为37℃。牛乳中添加葡萄糖和小分子氮源,可促进Lplantarum LT2-6生长和提高CLA产量。牛乳中亚油酸添加量超过0.075%时,对菌体生长和CLA生成均产生抑制。菌体生长对数期末尾和稳定期,CLA生成活跃。发酵24 h,CLA产量最大,生成的产物主要为cis-,trans-9,11-CLA。牛乳中加入0.075%的亚油酸,37℃,发酵24 h,亚油酸转化率为50%。脱脂牛乳添加亚油酸经L.plantarum LT2-6发酵后,CLA含量比普通酸乳提高5～6倍。
Conjugated linoleic acid (CLA) has attracted much attention as a novel type of biologically beneficial functional lipid. For example, CLA reduces carcinogenesis, atherosclerosis, and body fat. Presently, CLA is produced through chemical isomerization of linoleic acid (LA), but by-productions of unexpected isomers are produced, which is not beneficial for the use of CLA in food and medicine. However, CLA isomers are much simpler through biology isomerization of LA than through chemical. And it will bring a bright promise for application of CLA, which is isomerized by lactic acid bacteria.The process of CLA production by Lactobacillus plantarum was studied. The research included bacteria isolation, screening, identification and induced mutation, also fermentation dynamics, CLA production from washed cells, LA isomerase purification and its properties were studied, in the end the CLA concentration in milk was increased by fermentation. Results as the follows:1) Lactic acid bacteria L29, with a high ability to produce CLA, was isolated from pickle. L29 was identified as a strain ofLactobacillus plantarum (confidence level 96.1%) .2) Lplantarum L29 was induced by ultraviolet radiation and DES, then a mutation strain LT2-6 was screened out, by which CLA production could increase 18.6 %.3) Fermentation dynamics curve was obtained by the study of the Lplantarum LT2-6 fermentation in batches in 5 L fermentor. A mathematics model was established based on the cells growth of L. plantarum LT2-6, production CLA accumulation and glucose metabolism.4) Washed cells of Lplantarum LT2-6 as the catalyst, the optimal reaction condition for CLA production was: temperature 30℃, pH 7.0, LA concentration 1.5% (w/v) , cells concentration 20%(w/v). After reacted for 64 h, CLA concentration was up to the maximal (7.86 g/L), and 52.4 % of LA was conversed to CLA. Then fatty acid was extracted, methylated and determined by gas chromatography analysis, the majority of resultants was cis-,trans-9,ll-CLA.5) Molecular weight of the LA isomerase of Lplantarum LT2-6 was about 48.8 KDa. The optimal reaction condition for the LA isomerase was pH 6.0, 30℃. This enzyme was non-resistant to heat relatively, if temperature was up to 50℃, stabilization ability of the enzyme declined soon. Kinds of metal ion had different effects on the enzyme activity, lowering sequence was Mg2+ > Ca2+ > Mn2+ > Fe2+ > Zn2+ > Na+. Km and V,,,, dynamics parameters of LA isomerase, were respectively 12.46 mol/L, 1.933x10-1moVh.mg.6) In the process of fermentation of skim milk by Lplantarum LT2-6, 37℃ was suitable for fermentation. The growth of cells was accelerated significantly supplementing with glucose in milk, so production of CLA was increased. Also small molecular nitrogen source materials supplemented in milk had the same effects on CLA production. However, when LA concentration supplemented in milk was more than 0.075 %, the growth of cells and CLA production were restrained. Production of CLA was active during the end of log phase and stationary phase. Fermentation for 24 h, CLA production was the
maximum. Results showed that the majority of CLA in resultants was cis-,trans-9,ll-CLA. 0.075 % of LA supplemented in milk, and 50% of LA was bio-conversed into CLA in 37℃, fermentation for 24 h. After fermentation by Lplantarum LT2-6, CLA production was higher 5-6 times with skim milk supplemented LA than with market yoghourt without LA.
本课题对Lactobacillus plantarum转化亚油酸生成CLA过程进行了研究,内容包括菌种分离、筛选、鉴定,菌种诱变选育,CLA发酵过程动力学,洗涤细胞转化亚油酸生成CLA,亚油酸异构酶分离纯化,酶学性质研究和提高发酵牛乳CLA含量实验研究。论文主要内容如下:
1) 从酸菜中分离出一株较高CLA产量的乳酸菌L_(29),鉴定为植物乳杆菌(Lactobacillusplanmrum置信度96.1%)。
2) 以L.plantarum L_(29)为出发菌株,通过紫外线照射和硫酸二乙酯(DES)诱变处理,选育出突变株L.plantarum LT2-6,CLA产量提高18.6%。
3) 对L.planmrum LT2-6在5 L罐规模的分批发酵过程进行了实验分析研究,获得了分批发酵过程动力学曲线,建立了分批发酵过程菌体生长、产物CLA生成和底物(葡萄糖)代谢数学模型。
4) Lplanmrum LT2-6洗涤细胞转化亚油酸生成CLA优化条件为:30℃,pH 7.0,亚油酸浓度1.5%(w/v),细胞浓度20%(w/v)。反应64 h,CLA产量达到最大(7.86g/L),亚油酸转化率为52.4%。经脂肪酸萃取和甲酯化,气相色谱检测显示,土要产物为cis-,trans-9,11.CLA。
5) L.plantarum LT2-6亚油酸异构酶分子量约为48.8 KDa,最适pH 6.0,最适温度30℃,不耐热,温度高于50℃时,稳定性下降很快。金属离子对此酶活力影响顺序为:Mg~(2+)>Ca~(2+)>Mn~(2+)>Fe~(2+)>Zn~(2+)>Na~+。酶动力学参数为:K_m=12.46 μmol/L,V_m=1.933×10~(-1)/μmol/h.mg。
6) 以L.plantarum LT2-6为牛乳发酵剂,转化亚油酸生成CLA的适宜温度为37℃。牛乳中添加葡萄糖和小分子氮源,可促进Lplantarum LT2-6生长和提高CLA产量。牛乳中亚油酸添加量超过0.075%时,对菌体生长和CLA生成均产生抑制。菌体生长对数期末尾和稳定期,CLA生成活跃。发酵24 h,CLA产量最大,生成的产物主要为cis-,trans-9,11-CLA。牛乳中加入0.075%的亚油酸,37℃,发酵24 h,亚油酸转化率为50%。脱脂牛乳添加亚油酸经L.plantarum LT2-6发酵后,CLA含量比普通酸乳提高5～6倍。
Conjugated linoleic acid (CLA) has attracted much attention as a novel type of biologically beneficial functional lipid. For example, CLA reduces carcinogenesis, atherosclerosis, and body fat. Presently, CLA is produced through chemical isomerization of linoleic acid (LA), but by-productions of unexpected isomers are produced, which is not beneficial for the use of CLA in food and medicine. However, CLA isomers are much simpler through biology isomerization of LA than through chemical. And it will bring a bright promise for application of CLA, which is isomerized by lactic acid bacteria.The process of CLA production by Lactobacillus plantarum was studied. The research included bacteria isolation, screening, identification and induced mutation, also fermentation dynamics, CLA production from washed cells, LA isomerase purification and its properties were studied, in the end the CLA concentration in milk was increased by fermentation. Results as the follows:1) Lactic acid bacteria L29, with a high ability to produce CLA, was isolated from pickle. L29 was identified as a strain ofLactobacillus plantarum (confidence level 96.1%) .2) Lplantarum L29 was induced by ultraviolet radiation and DES, then a mutation strain LT2-6 was screened out, by which CLA production could increase 18.6 %.3) Fermentation dynamics curve was obtained by the study of the Lplantarum LT2-6 fermentation in batches in 5 L fermentor. A mathematics model was established based on the cells growth of L. plantarum LT2-6, production CLA accumulation and glucose metabolism.4) Washed cells of Lplantarum LT2-6 as the catalyst, the optimal reaction condition for CLA production was: temperature 30℃, pH 7.0, LA concentration 1.5% (w/v) , cells concentration 20%(w/v). After reacted for 64 h, CLA concentration was up to the maximal (7.86 g/L), and 52.4 % of LA was conversed to CLA. Then fatty acid was extracted, methylated and determined by gas chromatography analysis, the majority of resultants was cis-,trans-9,ll-CLA.5) Molecular weight of the LA isomerase of Lplantarum LT2-6 was about 48.8 KDa. The optimal reaction condition for the LA isomerase was pH 6.0, 30℃. This enzyme was non-resistant to heat relatively, if temperature was up to 50℃, stabilization ability of the enzyme declined soon. Kinds of metal ion had different effects on the enzyme activity, lowering sequence was Mg2+ > Ca2+ > Mn2+ > Fe2+ > Zn2+ > Na+. Km and V,,,, dynamics parameters of LA isomerase, were respectively 12.46 mol/L, 1.933x10-1moVh.mg.6) In the process of fermentation of skim milk by Lplantarum LT2-6, 37℃ was suitable for fermentation. The growth of cells was accelerated significantly supplementing with glucose in milk, so production of CLA was increased. Also small molecular nitrogen source materials supplemented in milk had the same effects on CLA production. However, when LA concentration supplemented in milk was more than 0.075 %, the growth of cells and CLA production were restrained. Production of CLA was active during the end of log phase and stationary phase. Fermentation for 24 h, CLA production was the
maximum. Results showed that the majority of CLA in resultants was cis-,trans-9,ll-CLA. 0.075 % of LA supplemented in milk, and 50% of LA was bio-conversed into CLA in 37℃, fermentation for 24 h. After fermentation by Lplantarum LT2-6, CLA production was higher 5-6 times with skim milk supplemented LA than with market yoghourt without LA.
引文
1.Ha YL, Grimm NK, Pariza MW. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid .Carcinogenesis. 1987.8:1881-1887
2.Ip C, Chin SF, Scimeca JA, et al. Mammary cancer prevention by conjugated dienoic derivative of linoleic acid.Cancer Res. 1991.51:6118-6124
3.Pariza MW, Hargraves WA. A beef derived mutagenesis modulator inhibits initiation of mouse epidermal tumrs by 7,12-dimethylbenz[a]anthracene. Carcinogenesis. 1985.6:591-593
4.Parodi PW. Conjugated linoleic acid-A anticarcinogenic fatty acid present in milk fat. Aust J Dairy Technol. 1994.49 (2): 93-97
5.Huang YC, Luedecke LO, Shultz TD. Effect of cheddar cheese consumption on plasma conjugated linoleic acid in men. Nutrition Res. 1994.14 (3): 373-386
6.Chin SF, Storkson JM, Liu W, et al. Conjugated linoleic acid (9,11-and 10,12-octadecadienoic acid) is produced in conventional but not germ-free rats fed linoleic acid.J Nutr. 1994.124:694-701
7. Pariza MW, Ashoor SH, Chu FS, et al. Effects of temperature and time on mutagen formation in pan- fried hamburger. Cancer Lett. 1979. 7:63-69
8. Parodi PW.Conjugated linoleic acid and other anticarcinogenic agents of bovine milk fat. J Dairy Sci 1999.82:1339-1349
9.Yurawecz MP, Hood JK, Roach JAG, et al. Conversion of allylic hydroxy oleate to conjugated inoleic acid and methoxy oleate by acid-catalyzed methylation procedures. J Am Oil Chem Soc. 1994.71 (10): 1149-1155
10.Adlof R, Lamm T. Fractionation of cis- and trans-oleic, linoleic, and conjugated linoleic fatty acid methyl esters by silver ion highperformance liquid chromatography. J Chromatogr. 1998.799:329- 332
11.Chin SF, Liu W, Storkson JM, et al. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J Food Compo Ana.1992.5 (3): 185-197
12 Parodi PW. Conjugated octadecadienoic acids of milk fax. J Dairy Sci. 1977. 60:1550-1553
13.Pardos SC, Torre PD, Sanchez-Muniz FJ. CLA antioxidant or prooxidant? Grasas Aceites. 2000.51:268-274
14.Oshea M, Lawless F, Stanton C, et al. Conjugated linoleic acid in bovine milk fat - a food-based approach to cancer chemoprevention. Trends Food Sci Technol. 1998. (9): 192-196
15.Juaneda P, Cordier O, Gregoire S, et al. Conjugated linoleic acid (CLA) isomers in heat-treated vegetable oils. OCL - Oleagineux Corps Gras Lipides. 2001.8 (1): 94-97
16. Werner SA, Luedecke LO, Shultz TD. Determination of conjugated linoleic acid content and isomer distribution in three Cheddar-type cheeses: effects of cheese culture, processing, and aging. J,Agric Food Chem. 1992.40 (10): 1817-1821
17.Shantha NC, Ram LN, O'Leary J, et al. Conjugated linoleic acid concentrations in dairy products as affected by processing and storage. J Food Sci. 1995.60 (4): 695-697
18.Nichols PL, Herb SF, Riemenschneider RW, Isomers of conjugated fatty acids Alkali-isomerized linoleic acid, J Am Chem Soc. 1951,73:247-252
19.Kepler CR, Hirons KP, Mcneill JJ, et al. Intermediates and products of the biohydrogenation of linoleic acid by Butyrivibrio fibrisolvens.J Biol Chem. 1966.241(6): 1350-1354
20.Ha YL, Storkson J, Pariza MW. Inhibition of benzo(a)pyrene-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Res. 1990.50:1097-1101
21.Ip C, Singh M, Thompson HJ, et al. Conjugated linoleic acid suppresses mammary carcinogenesis and proliferative activity of the mammary gland in the rat. Cancer Res. 1994.54:1212-1215
22. Pariza MW, Park Y, Kim S, et al. Mechanism of body fat reduction by conjugated linoleic acid. FASEB J. 1997.11:139-143
23.Pariza MW, Loretz LJ, Storkson, JM, et al. Mutagens and modulators of mutagenesis in fried ground beef .Cancer Res. 1983.43 (Suppl): 2444s-2446s.
24.Ip C. Review of the effects of trans fatty acids, oleic acid, n-3 polyunsaturated fatty acids, and conjugated linoleic acid on mammary carcinogenesis in animals.Am J Clin. Nutr.1997.66 (Suppl) :S1523-S1529
25.Ip C, Scimeca JA. Conjugated linoleic acid and linoleic acid are distinctive modulators of mammary carcinogenesis. Nutr Cancer. 1997. 27 (2): 131-135
26.Belury MA, Nickel KP, Bird CE, et al. Dietary conjugated linoleic acid modulation of phorbol ester skin tumor promotion. Nutr Cancer. 1996.26:149-157
27.Liew C, Schut HAJ, Chin SF, et al. Protection of conjugated linoleic acids against 2-amino-3-methy- limidazo [4,5-f] quinoline-induced colon carcinogenesis in the F344 rat: a study of inhibitory mechanisms. Carcinogenesis. 1995.16:3037-3043
28.Ip C, Briggs SP, Haegele AD, et al. The efficacy of conjugated linoleic acid in mammary cancer prevention is independent of the level or type of fat in the diet.Carcinogenssis 1996.17:1045-1050
29.Ip C, Jiang C, Thompson HJ, et al. Retention of conjugated linoleic acid in the mammary gland is associated with tumor inhibition during the post-initiation phase of carcinogenesis. Carcinogenesis. 1997.18:755-759
30.Liu KL, Belury MA. Conjugated linoleic acid modulation of phorbol ester-induced events in murine keratinocytes. Lipids. 1997.32:725-730
31.Liu KL, Belury MA. Conjugated linoleic acid reduces arachidonic acid content and PGE2 synthesis in murine keratinocytes. Cancer Lett. 1998.127:15-22
32.Shultz TD, Chew BP, Seaman WR. Differential stimulatory and inhibitory responses of human mcf-7 breast cancer cells to linoleic acid and conjugated linoleic acid in culture. Anticancer Res. 1992.12:2143-2145
33.Shultz TD, Chew BP, Seaman WR, et al. Inhibitory effect of conjugated dienoic derivatives of linoleic acid and beta-carotene on the in vitro growth of human cancer cells.Cancer Lett. 1992.63:125-133
34.Zu HX, Schut HA. Inhibition of 2-amino-3-methylimidazo[4,5-f]quinoline-DNA adduct formation in CDF1 mice by heat-altered derivatives of linoleic acid. Food Chem Toxicol. 1992.30:9-16
35.Schut HAJ, Cummings DA, Smale MHE, et al. DNA adducts of heterocyclic amines: Formation, removal and inhibition by dietary components. Mutat Res Fund. Mol Microbiol. 1997.376:185-194
36. Cunningham DC, Harrison LY, Shultz TD. Proliferative responses of normal human mammary and mcf-7 breast cancer cells to linoleic acid, conjugated linoleic acid and eicosanoid synthesis inhibitors in culture. Anticancer Res. 1997.17:197-203
37.Stangl GI. High dietary levels of a conjugated linoleic acid mixture alter hepatic glycerophospholipid class profile and cholesterol-carrying serum lipoproteins of rats. J Nutr Biochem.2000.11:184-191
38.Igarashi M, Miyazawa T. The growth inhibitory effect of conjugated linoleic acid on a human hepatoma cell line, HepG2, is induced by a change in fatty acid metabolism, but not the facilitation of lipid peroxidation in the cellsJBiochim Biophys Acta-Mol CellBiol Lipids.2001.1530:162-171
39.Cantwell H, Devery RO, Shea M, et al. The effect of conjugated linoleic acid on the antioxidant enzyme defense system in rat hepatocytes. Lipids.1999.34 (8): 833-839
40.Leung YH, Liu RH. trans-10,cis-12-conjugated linoleic acid isomer exhibits stronger oxyradical scavenging capacity than cis-9,trans-11-conjugated linoleic acid isomer. J Agric Food Chem. 2000. 48:5469-5475.
41.Mohan IK, Das UN. Prevention of chemically induced diabetes mellitus in experimental animals by polyunsaturated fatty acids. Nutrition.2001.17:126-151
42.Basu S, Smedman A, Vessby B. Conjugated linoleic acid induces lipid peroxidation in humans. FEBS Lett. 2000.468 (1): 33-36
43.Cook ME, Miller CC, Park Y, et al. Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. Poult Sci. 1993.72:1301-1305
44.Lee KN, Kritchevsky D, Pariza MW. Conjugated linoleic acid and atherosclerosis in rabbitsAtheros- clerosis. 1994.108:19-25
45.Nicolosi RJ, Rogers EJ, Kritchevsky D, et al. Dietary conjugated linoleic acid reduces plasma and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery. 1997.22:266-277
46.Truitt A, McNeill G, Vanderhoek JY. Antiplatelet effects of conjugated linoleic acid isomers. Biochim. Biophys Acta. 1999.1438:239-246
47.Dugan MER, Aalhus JL, Jeremiah LE, et al. The effects of feeding conjugated linoleic acid on subsequent pork quality. CanAnim Sci.1999. 79.45-51
48.Park Y, Albright KJ, Liu W, et al. Effect of conjugated linoleic acid on body composition in mice. Lipids. 1997.32:853-858
49.Sisk M, Hausman D, Martin R, et al. Dietary conjugated linoleic acid reduces adposity in lean but not obese Zucker rats. J Nutr. 2001.131:1668-1674
50.Smedman A, Vessby B. Conjugated linoleic acid supplementation in humans metabolic effects. J Nutr. 2001.36:773-781
51.Park Y, Albright KJ, Storkson JM, et al. Changes in body composition in mice during feeding and withdrawal of conjugated linoleic acid. Lipids. 1999.34:243-248
52.Lehmann JM, Moore LB, Smith OTA, et al. An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPARy). J Biol Chem. 1995.270: 12953-12956
53.Tsuboyama KN, Takahashi M, Tanemura K, et al. Conjugated linoleic acid supplementation reduces adipose tissue by apoptosis and develops lipodystrophy in mice. Diabetes.2000.49:1534-1542
54.Park Y, Storkson JM, Albright KJ, et al. Evidence that the trans-10, cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids. 1999. 34:235-241
55.Choi Y, Kim YC, Han YB, et al.The trans-10,cis-12 isomer of conjugated linoleic acid downregulates stearoyl-CoA desaturase 1 gene expression in 3T3-L1 adipocytes. Nutr. 2000.130:1920-1924
56. West DB, Delany JP, Camet PM, et al. Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am.J. Physiol Regulatory Integrative Comp. Physiol. 1998.44.R667-R672
57.0hnuki K, Haramizu S, Oki K, et al. A single oral administration of conjugated linoleic acid enhanced energy metabolism in mice. Lipids. 2001.37:583-587
58.de Deckeri EAM, van Amesvoort JMM, McNeill GP, et al. Effects of conjugated linoleic acid (CLA)
isomers on lipid levels and peroxisome proliferation in the hamster Br Nutr. 1999.82.309-317
59.Martin JC, Gregoire S, Siess MH, et al. Effects of conjugated linoleic acid isomers on lipid metabolizing enzymes in male rats. Lipids. 2000.35:91-98
60.Evans M, Pariza M, Park Y, et al. Trans-10,cis-12 conjugated linoleic acid reduces triglyceride content while differentially affecting peroxisome proliferator activated receptor-γ2 and aP2 expression in 3T3-L1 preadipocytes. Lipids. 2000.36:1223-1232
61.Houseknecht KL, Vanden HJP, Moya CSY, et al. Dieop conjugated linoleic acd normalizes impaired glucose tolerance in the Zucker diabetic fatty fa/fa rat. Biochem Biophys Res Commun.1998.244: 678-682
62.Schoonjans K, Staels B, Anwerx J. The peroxisome proliferator activated receptors (PPARs) and their effects on lipid metabolism and adpocyte differentiation. Biochim Biophys Acta.1996. 1302: 93-109
63.Satory DL, Smith SB. Conjugated linoleic acid inhibits proliferation but stimulates lipid filling of murine 3T3-L1 preadipocytes. J Nutr. 1998.129:92-97
64. Park Y, Pariza MW. The effects of dietary conjugated nonadecadienoic acid on body composition in mice. Biochim Biophys Acta.2001.1533:171-174
65.Blankson H, Stakkstad J, Fagertun H, et al.Conjugated linoleic acid reduces body fat mass in overweight and obese humans. Nutr. 2000.130:2943-2948
66.Thom E, Wadstein J, Gudmundson O. Conjugated linoleic acid reduces body fat in healthy exercising humans. J Int Med Res. 2001. 29:392-396
67.Yang M, Pariza MW, Cook ME. Dietary conjugated linoleic acid Protects against end stage disease of lupus erythematosus in the NZB/W FI mouse. Immunopharmacol Immunotoxicol.2000.22:433- 449
68.Belury MA, Kempa SA. Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids. 1997.32:199-204
69.Moya CSY, Vanden HJP, Belury MA. Conjugated linoleic acid activates peroxisome proliferator activated receptor α and β subtypes but does not induce hepatic peroxisome proliferation in Sprague Dawley rats. Biochim Biophys Acta. 1999.1436:331-342
70.Moya CSY, Vanden HJP, Belury MA. Conjugated linoleic acd is a potent naturally occuring ligand and activator of PPARa Lipids Res. 1999.40:1426-1433
71.Banni S, Carta G, Angioni E, et al. Distribution of conjugated linoleic acid and metabolites in different lipid fractions in the rat liverlipid Res. 2001.42:1056-1061
72.Kavanaugh CJ, Liu KL, Belury MA. Effect of dietary conjugated linoleic acid on phorbol ester induced PGE2 production and hyperplasia in mouse epidermis. Nutr Cancer.1999.33:132-138
73.Li Y, Watkins BA. Conjugated linoleic acids alter bone fatty acid composition and reduce ex vivo prostaglandin E2 biosynthesis in rats fed n-6 or n-3 fatty acids. Lipids. 1998.33:417-425
74.IP C, Banni S, Angioni E, et al. Conjugated linoleic acid-enriched butter fat alters mammary gland morphogenesis and reduces cancer risk in rats. J Nutr. 1999.129.2135-2142
75.Eggert JM, Belury MA, Kempa SA, et al. Effects of conjugated linoleic acid on the belly firmness and fatty acid composition of genetically lean pigs Anim Sci. 2002.79:2866-2872
76.Sugano M, Tsujita A, Yamasaki M, et al.Conjugated linoleic acid modulates tissue levels of chemical mediator and immunoglobulins in rats Lipids. 1998.33:521-527
77. Liu KL, Belury MA. Conugated linoleic acid reduces arachidonic acid content and PGE2 synthesis in murine keratinocytes. Cancer Lett. 1998.124:1-8
78.Belury MA, Moya CSY, Liu KL.et al. Conjugated Iinoleic acid induces peroxisome proliferators associated enzyme expression and ornithine decarboxylase activity in mouse liver. Nutr Biochem. 1997.8:579-583
79.Bulgarella J, Patton D, Bull A. Modulation of Prostaglandin H synthase activity by conjugated Iinoleic acid (CLA) and specific CLA isomes.Lipids.2001. 36:407-512
80.Park Y, Storkson JM, Ntambi JM, et al. Inhibition of hepatic stearoyl-CoA desaturase activity by trans-10, cis-12 conjugated Iinoleic acid and its derivativesiBiochim Biophys Acta. 2000.1486:285-292
81.Sisk M, Hausman D, Martin R, et al. Dietary conjugated Iinoleic acid reduces adposity in lean but not obese Zucker rats. J Nutr. 2001.131:1668-1674
82.Lee KN, Pariza MW, Ntambi JM. Conjugated Iinoleic acid decreases hepatic stearoyl-CoA desaturase mRNA expression. Biochem. Biophys. Res Commum. 1998.248:817-821
83.Banni S, Angioni E, Casu V, et al. Decrease in Iinoleic acid metabolites as a potential mechanism in cancer risk reduction by conjugated Iinoleic acid. Carcinogenesis. 1999.20:1019-1024
84.Poulos SP, Sisk M, Hausman DB, et al. Pre- and postnatal dietary conjugated Iinoleic acid alters adipose development, body weigh gain and body composition in Sprague-Dawley rats. J Nutr. 2001. 131:2722-2731
85.Ntambi JM, Kim YC. Adipocyte differentiation and gene exprssion.J Nutr .2000.130:3122s-3126s
86.Belury MA, Moya CSY, Lu M, et al. Conjugated Iinoleic acid is an activator and ligand for peroxisome proliferators activated receptor gamma (PPAR gamma). Nutr Res. 2002.22 (7):817~824
87.Ryder JW, Portocarrero CP, Song XM, et al. Isomerspecific antidiabetic properties of conjugated Iinoleic acid. Diabetes.200l.6Q: 1149-1157
88.Sporn GI, Muller H, Kirchgessner M. Conjugated Iinoleic acid effects on circulating hormones, metabolites and lipoproteins, and its proportion in fasting serum and erythrocyte membranes of swine. Eur. J Nutr. 1999.38:271-277
89.Belury MA. Dietary conjugated Iinoleic acid in health: Physiological effects and mechanisms of action. Ann. Rev Nutr.2002.22:505-531
90.Chew BP, Wong TS, Shultz TD, et al. Effects of conjugated dienoic derivatives of Iinoleic acid and beta-carotene in modulating lymphocyte and macrophage function. Anticancer Res. 1997.17:1099- 1106
91. Wong MW, Chew BP, Wong TS, et al.Effects of dietary conjugated Iinoleic acid on lymphocyte function and growth of mammary tumors in mice. Anticancer Res. 1997.17:987-993
92.Hayek MG, Han SN, Wu DY, et al. Dietary conjugated Iinoleic acid influences the immune response of young and old C57BL/6NCrlBR mice. J Nutr. 1999.129:32-38
93.Bassaganya RJ, Hontecillas MR, Zimmerman DR. et al. Dietary conjugated Iinoleic acid modula- tes phenotype and effector functions of porcine CD8 (+) lymphocytes. J Nutr. 2001.131:2370- 2377
94.Bassaganya RJ, Hontecillas MR, Bregendahl K, et al. Effects of dietary conjugated Iinoleic acid in nursery pigs of dirty and clean environments on growth, empty body composition, and immune competence. J Anim Sci.2001.79:714-721
95.Bassaganya RJ, Hontecillas R, Zimmerman DR, et al. Long-term influence of lipid nutrition on the induction of CD8(+) responses to viral or bacterial antigens. Vaccine 2002.20 (9-10): 1435-1444
96.Cook, ME, Jerome DL, Pariza, MW. Broilers fed conjugated Iinoleic acid had enhanced bone ash. Poultry Science. 1997.76 (Supp.l):41-46
97.Watkins BA, Seifert MF. Conjugated Iinoleic acid and bone biology. J Am Coll Nutr. 2000.19:478S- 486S
98. Doyle E. Scientific forum explores CLA knowledge. INFORM. 1998. 9 (1): 69-73
99. Dugan MER, Aalhus JL, Schaefer AL, et al. The effect of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs. Can J Anim Sci. 1997. 77: 723-725
100. Ostrowska E, Muralitharan M, Cross RF, et al. Dietary conjugated linoleic acids increase lean tissue and decrease fat deposition in growing pigs. J Nut. 1999. 129: 2037-2042
101. Bee G. Dietary conjugated linoleic acid consumption during pregnancy and lactation influences growth and tissue composition in weaned pigs. J Nutr. 2000. 130: 2981-2989
102. Belury MA. Conjugated dienoic linoleate: a polyunsaturated fatty acid with enique chemoprotective properties. Nutr Rev. 1995. 53: 83-89
103. Banni S, Day BW, Evans RW, et al. Detection of conjugated diene isomers of linoleic acid in liver lipids of rats fed a cholinedevoid diet indicates that the diet does not cause lipoperoxidation. Nutr Biochem. 1995. 6: 281-89
104. Brown M, Evans M, Mcintosh M. Linoleic acid partially restores the triglyceride content of conjugated linoleic acid-treated cultures of 3T3-L1 preadipocytes. Nutr Biochem. 2001. 12: 381-87
105. Palmquist DL. Ruminal and endogenous synthesis of CLA in cows. Aust. J Dairy Technol. 2001. 56 (2): 134-137
106. Benjamin. AC, Lance. HB, Debra. AD, et al. The role of △9-desaturase in the production of cis-9, trans-11CLA J Nutr Biochem. 2001. 12: 622-630
107. Lin TY, Lin CW, Lee CH. Conjugated linoleic acid concentration as affected by lactic cultures and added linoleic acid. Food Chem. 1999. 67: 1-5
108. Griinari JM, Bauman DE. Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In Advances in Conjugated Linoleic Acid Research, Vol. 1. Champaign IL AOCS Press. 1999. pp. 180-200
109. Corl BA, Baumgard LH, Dwyer DA, et al. The role of △9-desaturase in the production of cis-9, trans-11 CLA. J Nutr Biochem. 2001. 12: 622-630
110. Cook ME, Pariza MW. Dietetic Food Containing Conjugated Linoleic Acid US Patent. 1998. 5, 760, 082
111. Krajca KE, Flow Process for Conjugated Unconjugated Unsaturation of Fatty Acids. US Patent. 1979. 4, 164, 505
112. Iwata T, Kamegai T, Sato Y, et al. Methed for Production Conjugated Linoleic Acid. US Patent. 1999. 5, 986, 116,
113. Saebo A, Skarie C, Jerome D. et al. Conjugated Linoleic Acid compositions and Methods of Making Same. US Patent. 2002. 6, 410, 761,
114.刘春奎.共轭亚油酸的生产方法.中国专利:2001.CN1289785A
115. Reaney MJ, Liu YD, Westcott ND, Method for Commercial Preparation of Conjugated Linoleic Acid. US Patent. 2002. 6, 420, 577,
116.李光友,刘发义,石红旗,微胶囊化共轭亚油酸.中国专利:2001.CNI287114A,
117. Seidel MC. Liquid Chromatography of High Purity Conjugated Fatty Acid. US Patent. 2002. 6, 410, 763,
118. Basu A, Bhaduri S. Process for the Manufacture of Conjugated Polyenoic Fatty Compounds. US Patent. 1985. 0, 160, 544,
119. Sleeter RT. Method of Conjugated Double Bonds in Drying Oils. US Patent. 1998. 5, 719, 301
120. Christie WW, Dobson G, Gunstone FD. Isomers in commercial samples of conjugated linoleic acid. Lipids. 1997. 32: 1231-1235
121. Kim YJ, Liu RH, Bond DR, et al. Effect of linoleic acid concentration on conjugated linoleic acid production by Butyrivibrio fibrisolvens A38. Appl Environ Microbiol. 2000. 66 (12): 5226-5230
122. Kim YJ, Liu RH, Rychlik JL, et al. The enrichment of a ruminal bacterium (Megasphaera elsdenii YJ-4) that produces the trans-10, cis-12 isomer of conjugated linoleic acid. J Appl Microbiol. 2002. 92: 976-982
123. Jiang J, Bjorck L, Fonden R. Production of conjugated linoleic acid by dairy starter cultures. J Appl Microbiol. 1998. 85: 95-102
124. Rainio A, Vahvaselka M, Suomalainen T, et al. Reduction of linoleic acid inhibition in production of conjugated linoleic acid by Propionibacterium freudenreichii ssp shermanii. Can J Microbiol. 2001. 47: 735-740
125. Rainio A, Vahvaselka M, Suomalainen T, et al. Production of conjugated linoleic acid by Propionibacterium freudenreichii ssp shermanii. Lait. 2002. 82 (1): 91-101
126. Lin TY. Conjugated linoleic acid concentration as affected by lactic cultures and additives. Food Chem. 2000. 69: 27-31
127. Lin TY, Lin CW, Wang YJ. Linoleic acid isomerase activity in enzyme extracts from Lactobacillus acidophilus and Propionibacterium freudenreichii ssp shermanii. J Food Sci. 2002. 67(4): 1502-505
128. Pariza, MW. Yang XY. Method of producing conjugated fatty acids. US Patent. 2000. 6, 060, 304.
129. Kim YJ, Liu RH, Increase of conjugated linoleic acid content in milk by fermentation with lactic acid bacteria. J Food Sci. 2002. 67 (5): 1731-1737
130. Ham JS, In YM, Jeong SG, et al. Screening of conjugated linoleic acid producing lactic acid bacteria from fecal samples of healthy babies. Asian-Australasian J Anim Sci. 2002. 15 (7):1031-1035
131. Ogawa J, Matsumura K, Kishino S, et al. Conjugated linoleic acid accumulation via 10-hydroxy-12 octadecaenoic acid during microaerobic transformation of linoleic acid by Lactobacillus acidophilus. Appl Environ Microbiol. 2001. 67 (3): 1246-1252
132. Kishino S, Ogawa J, Omura Y, et al. Conjugated linoleic acid production from linoleic acid by lactic acid bacteria. J Am Oil Chem Soc. 2002. 79 (2): 159-163
133.陈忠周,李艳梅,赵刚等.共轭亚油酸的性质及合成.中国油脂.2000.25(5):41—45
134. Pariza, MW, Yang XY, Method of producing conjugated fatty acids. US Patent. 1999. 5, 856, 149
135.邵群,边际,马丽等.乳酸菌发酵生产共轭亚油酸条件的研究.山东师范大学学报(自然科学版)2001,16(4):443-446
136.曹键,魏明,曾实等.一株嗜酸乳杆菌突变株转化亚油酸为共轭亚油酸条件的研究.食品科学2003,24(9):76-79
137.熊向峰,陈超银,赵声兰等.天然植物油碱异构化合成共轭亚油酸的研究.中国油脂2003,28(4):39-41
138.张亚刚,樊莉,文彬等.紫外可见分光光度法在共轭亚油酸定量分析中的应用.新疆石油学院学报2002,14(2):59—64
139.张亚刚,樊莉,马莉等.用紫外可见分光光度法测定共轭亚油酸的含量.中国科学院研究生院学报 2002,19(4):436-442
140.吴冀华,裘爱泳.共轭亚油酸的分析.中国油脂,2002,27(2):12-13
141. Kishino S, Ogawa J, Ando A, et al. Structural Analysis of Conjugated Linoleic Acid Produced by Lactobacillus plantarum, and Factors Affecting Isomer Production. Biosci Biotechnol Biochem. 2003. 67 (1): 179-182
142. Fogerty AC, Ford GL, Svoronos D. Octadeca-9, 11-dienoic acid in food stuffs and in the lipids of human blood and breast milk. Nutr Rep Int. 1988. 38: 937-944
143.凌代文,东秀珠.乳酸细菌分类鉴定及实验方法.北京:中国轻工业出版社,1999,3:85-129
144.钱存柔,黄仪秀.微生物学实验教程.北京:北京大学出版社,1999
145. Wijtzes T, Bruggeman M R, Nout M J. et al. A computerised system for the identification of lactic acid bacteria. International Food Microbiology. 1997. 38 (1): 65-70
146.杜连祥等.工业微生物实验技术.天津:天津科技出版社,1992
147.姚汝华.微生物工程工艺原理.广州:华南理工大学出版社,1996
148.戚以政,汪叔雄.生化反应动力学与反应器.北京:化学工业出版社,1999,第2版
149.天津轻工业学院等编.工业发酵分析.北京:轻工业出版社,1980
150. Bessa RJB, Santos S J, Ribeiro JMR, et al. Reticulorumen biohydrogenation and the enrichment of ruminant edible products with linoleic acid conjugated isomers. Livestock Prod Sci. 2000. 63: 201-211
151.中华人民共和国国家质量技术监督局.GB/T17376-1998.中华人民共和国国家标准—动植物油脂脂肪酸甲酯制备.北京:中国标准出版社,1998-05-08
152. Roach JAG, Mossoba MM, Yurawecz MP, et al. Chromatographic separation and identification of conjugated linoleic acid isomers. Analytica Chimica Acta. 2002. 465: 207-226
153.中华人民共和国国家质量技术监督局.GB/T 17377-1998动植物油脂 脂肪酸甲酯的气相色谱分析.北京:中国标准出版社,1998-05-08
154. Kishino S, Ogawa J, Ando A, et al. Conjugated alpha-linolenic acid production from a-linolenic linolenic acid by Lactobacillus plantarum AKU 1009a. European J Lipid Sci Technol. 2003. 105 (10): 572-577
155.吴冀华,裘爱泳.气相色谱法分析共轭亚油酸异构体.中国油脂,2002,27(1):65-67
156. Rosson RA, Grund AD, et al. Linoleate Isomerase. World Patent. 2001. 0100846,
157.汪家政,范明.蛋白质技术手册.北京:科学出版社,2001,189-210
158.蔡武城,李碧羽,李玉民.生物化学实验技术教程.上海:复旦大学出版社,1983,1-8
159.曹键,魏明,曾实等.一株嗜酸乳杆菌突变株亚油酸异构酶的纯化及性质.食品与发酵工业 2004,30(2):48-52
160. Vant VP, Dekker JM, Larmars JWJ, et al. Consumption of fermented milk products and breast cancer: A case-control study in the Netherlands. Cancer Res. 1989. 49: 4020-4023
161. Mccarthy AC, La E, Conti CJ. et al. Effect of spray-dried yogurt and lactic acid bacteria on the initiation and promotion stages of chemically induced skin carcinogenesis in mice. Nutr Cancer. 1997. 27: 231-237
162. Kimura KA, Mccartneym A, Mcconnel M, et al. Analysis of fecal populations of bifidobacteria and latobacilli and investigation of the immunological response of their human host to the predominant strains. Appl Environ Microbiol. 1997. 63: 3394-3398
163. Xue L, Lipkin M, Newmark H, et al. Influence of dietary calcium and vitamin D on diet-induced epithelial cell hyperproliferation in mice. J Nat Cancer Inst. 1999. 91: 176—181
164. Cassand P, LevEque F, Decoudu S, et al. Relationship between vitamin A dietary intake and mutagenic activity of some chemical carcinogens, Food Addit Contam. 1990. 7:S58-S59
165. Dhiman TR, Helmink ED, McMahon DJ, et al. Conjugated linoleic acid content of milk and cheese from cows fed extruded oilseeds. J Dairy Sci. 1999, 82: 412-419
166. Kelly ML, Kolver ES, Bauman DE, et al. Effect of intake of pasture on concentrations of conjugated linoleic acid in milk of lactating cows. J Dairy Sci. 1998. 81: 1630-1636
167. McGuire MA, Duckett SK, Andrae G, et al. Effect of high-oil corn on content of conjugated linoleic acid (CLA) in beef. J Dairy Sci. 1998. 81: 301-309
168.杜连祥,赵征,王敏第.乳酸菌及其发酵制品生产技术.天津:天津科学技术出版社,1999,91-95
169.金世林编.乳与乳制品生产.北京:中国轻工业出版社,1990
170. Ando A, Ogawa J, Kishino S, et al. CLA production from ricinoleic acid by lactic acid bacteria. J. Am. Oil Chem. Soc. 2003. 80 (9): 889-894
2.Ip C, Chin SF, Scimeca JA, et al. Mammary cancer prevention by conjugated dienoic derivative of linoleic acid.Cancer Res. 1991.51:6118-6124
3.Pariza MW, Hargraves WA. A beef derived mutagenesis modulator inhibits initiation of mouse epidermal tumrs by 7,12-dimethylbenz[a]anthracene. Carcinogenesis. 1985.6:591-593
4.Parodi PW. Conjugated linoleic acid-A anticarcinogenic fatty acid present in milk fat. Aust J Dairy Technol. 1994.49 (2): 93-97
5.Huang YC, Luedecke LO, Shultz TD. Effect of cheddar cheese consumption on plasma conjugated linoleic acid in men. Nutrition Res. 1994.14 (3): 373-386
6.Chin SF, Storkson JM, Liu W, et al. Conjugated linoleic acid (9,11-and 10,12-octadecadienoic acid) is produced in conventional but not germ-free rats fed linoleic acid.J Nutr. 1994.124:694-701
7. Pariza MW, Ashoor SH, Chu FS, et al. Effects of temperature and time on mutagen formation in pan- fried hamburger. Cancer Lett. 1979. 7:63-69
8. Parodi PW.Conjugated linoleic acid and other anticarcinogenic agents of bovine milk fat. J Dairy Sci 1999.82:1339-1349
9.Yurawecz MP, Hood JK, Roach JAG, et al. Conversion of allylic hydroxy oleate to conjugated inoleic acid and methoxy oleate by acid-catalyzed methylation procedures. J Am Oil Chem Soc. 1994.71 (10): 1149-1155
10.Adlof R, Lamm T. Fractionation of cis- and trans-oleic, linoleic, and conjugated linoleic fatty acid methyl esters by silver ion highperformance liquid chromatography. J Chromatogr. 1998.799:329- 332
11.Chin SF, Liu W, Storkson JM, et al. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J Food Compo Ana.1992.5 (3): 185-197
12 Parodi PW. Conjugated octadecadienoic acids of milk fax. J Dairy Sci. 1977. 60:1550-1553
13.Pardos SC, Torre PD, Sanchez-Muniz FJ. CLA antioxidant or prooxidant? Grasas Aceites. 2000.51:268-274
14.Oshea M, Lawless F, Stanton C, et al. Conjugated linoleic acid in bovine milk fat - a food-based approach to cancer chemoprevention. Trends Food Sci Technol. 1998. (9): 192-196
15.Juaneda P, Cordier O, Gregoire S, et al. Conjugated linoleic acid (CLA) isomers in heat-treated vegetable oils. OCL - Oleagineux Corps Gras Lipides. 2001.8 (1): 94-97
16. Werner SA, Luedecke LO, Shultz TD. Determination of conjugated linoleic acid content and isomer distribution in three Cheddar-type cheeses: effects of cheese culture, processing, and aging. J,Agric Food Chem. 1992.40 (10): 1817-1821
17.Shantha NC, Ram LN, O'Leary J, et al. Conjugated linoleic acid concentrations in dairy products as affected by processing and storage. J Food Sci. 1995.60 (4): 695-697
18.Nichols PL, Herb SF, Riemenschneider RW, Isomers of conjugated fatty acids Alkali-isomerized linoleic acid, J Am Chem Soc. 1951,73:247-252
19.Kepler CR, Hirons KP, Mcneill JJ, et al. Intermediates and products of the biohydrogenation of linoleic acid by Butyrivibrio fibrisolvens.J Biol Chem. 1966.241(6): 1350-1354
20.Ha YL, Storkson J, Pariza MW. Inhibition of benzo(a)pyrene-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Res. 1990.50:1097-1101
21.Ip C, Singh M, Thompson HJ, et al. Conjugated linoleic acid suppresses mammary carcinogenesis and proliferative activity of the mammary gland in the rat. Cancer Res. 1994.54:1212-1215
22. Pariza MW, Park Y, Kim S, et al. Mechanism of body fat reduction by conjugated linoleic acid. FASEB J. 1997.11:139-143
23.Pariza MW, Loretz LJ, Storkson, JM, et al. Mutagens and modulators of mutagenesis in fried ground beef .Cancer Res. 1983.43 (Suppl): 2444s-2446s.
24.Ip C. Review of the effects of trans fatty acids, oleic acid, n-3 polyunsaturated fatty acids, and conjugated linoleic acid on mammary carcinogenesis in animals.Am J Clin. Nutr.1997.66 (Suppl) :S1523-S1529
25.Ip C, Scimeca JA. Conjugated linoleic acid and linoleic acid are distinctive modulators of mammary carcinogenesis. Nutr Cancer. 1997. 27 (2): 131-135
26.Belury MA, Nickel KP, Bird CE, et al. Dietary conjugated linoleic acid modulation of phorbol ester skin tumor promotion. Nutr Cancer. 1996.26:149-157
27.Liew C, Schut HAJ, Chin SF, et al. Protection of conjugated linoleic acids against 2-amino-3-methy- limidazo [4,5-f] quinoline-induced colon carcinogenesis in the F344 rat: a study of inhibitory mechanisms. Carcinogenesis. 1995.16:3037-3043
28.Ip C, Briggs SP, Haegele AD, et al. The efficacy of conjugated linoleic acid in mammary cancer prevention is independent of the level or type of fat in the diet.Carcinogenssis 1996.17:1045-1050
29.Ip C, Jiang C, Thompson HJ, et al. Retention of conjugated linoleic acid in the mammary gland is associated with tumor inhibition during the post-initiation phase of carcinogenesis. Carcinogenesis. 1997.18:755-759
30.Liu KL, Belury MA. Conjugated linoleic acid modulation of phorbol ester-induced events in murine keratinocytes. Lipids. 1997.32:725-730
31.Liu KL, Belury MA. Conjugated linoleic acid reduces arachidonic acid content and PGE2 synthesis in murine keratinocytes. Cancer Lett. 1998.127:15-22
32.Shultz TD, Chew BP, Seaman WR. Differential stimulatory and inhibitory responses of human mcf-7 breast cancer cells to linoleic acid and conjugated linoleic acid in culture. Anticancer Res. 1992.12:2143-2145
33.Shultz TD, Chew BP, Seaman WR, et al. Inhibitory effect of conjugated dienoic derivatives of linoleic acid and beta-carotene on the in vitro growth of human cancer cells.Cancer Lett. 1992.63:125-133
34.Zu HX, Schut HA. Inhibition of 2-amino-3-methylimidazo[4,5-f]quinoline-DNA adduct formation in CDF1 mice by heat-altered derivatives of linoleic acid. Food Chem Toxicol. 1992.30:9-16
35.Schut HAJ, Cummings DA, Smale MHE, et al. DNA adducts of heterocyclic amines: Formation, removal and inhibition by dietary components. Mutat Res Fund. Mol Microbiol. 1997.376:185-194
36. Cunningham DC, Harrison LY, Shultz TD. Proliferative responses of normal human mammary and mcf-7 breast cancer cells to linoleic acid, conjugated linoleic acid and eicosanoid synthesis inhibitors in culture. Anticancer Res. 1997.17:197-203
37.Stangl GI. High dietary levels of a conjugated linoleic acid mixture alter hepatic glycerophospholipid class profile and cholesterol-carrying serum lipoproteins of rats. J Nutr Biochem.2000.11:184-191
38.Igarashi M, Miyazawa T. The growth inhibitory effect of conjugated linoleic acid on a human hepatoma cell line, HepG2, is induced by a change in fatty acid metabolism, but not the facilitation of lipid peroxidation in the cellsJBiochim Biophys Acta-Mol CellBiol Lipids.2001.1530:162-171
39.Cantwell H, Devery RO, Shea M, et al. The effect of conjugated linoleic acid on the antioxidant enzyme defense system in rat hepatocytes. Lipids.1999.34 (8): 833-839
40.Leung YH, Liu RH. trans-10,cis-12-conjugated linoleic acid isomer exhibits stronger oxyradical scavenging capacity than cis-9,trans-11-conjugated linoleic acid isomer. J Agric Food Chem. 2000. 48:5469-5475.
41.Mohan IK, Das UN. Prevention of chemically induced diabetes mellitus in experimental animals by polyunsaturated fatty acids. Nutrition.2001.17:126-151
42.Basu S, Smedman A, Vessby B. Conjugated linoleic acid induces lipid peroxidation in humans. FEBS Lett. 2000.468 (1): 33-36
43.Cook ME, Miller CC, Park Y, et al. Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. Poult Sci. 1993.72:1301-1305
44.Lee KN, Kritchevsky D, Pariza MW. Conjugated linoleic acid and atherosclerosis in rabbitsAtheros- clerosis. 1994.108:19-25
45.Nicolosi RJ, Rogers EJ, Kritchevsky D, et al. Dietary conjugated linoleic acid reduces plasma and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery. 1997.22:266-277
46.Truitt A, McNeill G, Vanderhoek JY. Antiplatelet effects of conjugated linoleic acid isomers. Biochim. Biophys Acta. 1999.1438:239-246
47.Dugan MER, Aalhus JL, Jeremiah LE, et al. The effects of feeding conjugated linoleic acid on subsequent pork quality. CanAnim Sci.1999. 79.45-51
48.Park Y, Albright KJ, Liu W, et al. Effect of conjugated linoleic acid on body composition in mice. Lipids. 1997.32:853-858
49.Sisk M, Hausman D, Martin R, et al. Dietary conjugated linoleic acid reduces adposity in lean but not obese Zucker rats. J Nutr. 2001.131:1668-1674
50.Smedman A, Vessby B. Conjugated linoleic acid supplementation in humans metabolic effects. J Nutr. 2001.36:773-781
51.Park Y, Albright KJ, Storkson JM, et al. Changes in body composition in mice during feeding and withdrawal of conjugated linoleic acid. Lipids. 1999.34:243-248
52.Lehmann JM, Moore LB, Smith OTA, et al. An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPARy). J Biol Chem. 1995.270: 12953-12956
53.Tsuboyama KN, Takahashi M, Tanemura K, et al. Conjugated linoleic acid supplementation reduces adipose tissue by apoptosis and develops lipodystrophy in mice. Diabetes.2000.49:1534-1542
54.Park Y, Storkson JM, Albright KJ, et al. Evidence that the trans-10, cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids. 1999. 34:235-241
55.Choi Y, Kim YC, Han YB, et al.The trans-10,cis-12 isomer of conjugated linoleic acid downregulates stearoyl-CoA desaturase 1 gene expression in 3T3-L1 adipocytes. Nutr. 2000.130:1920-1924
56. West DB, Delany JP, Camet PM, et al. Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am.J. Physiol Regulatory Integrative Comp. Physiol. 1998.44.R667-R672
57.0hnuki K, Haramizu S, Oki K, et al. A single oral administration of conjugated linoleic acid enhanced energy metabolism in mice. Lipids. 2001.37:583-587
58.de Deckeri EAM, van Amesvoort JMM, McNeill GP, et al. Effects of conjugated linoleic acid (CLA)
isomers on lipid levels and peroxisome proliferation in the hamster Br Nutr. 1999.82.309-317
59.Martin JC, Gregoire S, Siess MH, et al. Effects of conjugated linoleic acid isomers on lipid metabolizing enzymes in male rats. Lipids. 2000.35:91-98
60.Evans M, Pariza M, Park Y, et al. Trans-10,cis-12 conjugated linoleic acid reduces triglyceride content while differentially affecting peroxisome proliferator activated receptor-γ2 and aP2 expression in 3T3-L1 preadipocytes. Lipids. 2000.36:1223-1232
61.Houseknecht KL, Vanden HJP, Moya CSY, et al. Dieop conjugated linoleic acd normalizes impaired glucose tolerance in the Zucker diabetic fatty fa/fa rat. Biochem Biophys Res Commun.1998.244: 678-682
62.Schoonjans K, Staels B, Anwerx J. The peroxisome proliferator activated receptors (PPARs) and their effects on lipid metabolism and adpocyte differentiation. Biochim Biophys Acta.1996. 1302: 93-109
63.Satory DL, Smith SB. Conjugated linoleic acid inhibits proliferation but stimulates lipid filling of murine 3T3-L1 preadipocytes. J Nutr. 1998.129:92-97
64. Park Y, Pariza MW. The effects of dietary conjugated nonadecadienoic acid on body composition in mice. Biochim Biophys Acta.2001.1533:171-174
65.Blankson H, Stakkstad J, Fagertun H, et al.Conjugated linoleic acid reduces body fat mass in overweight and obese humans. Nutr. 2000.130:2943-2948
66.Thom E, Wadstein J, Gudmundson O. Conjugated linoleic acid reduces body fat in healthy exercising humans. J Int Med Res. 2001. 29:392-396
67.Yang M, Pariza MW, Cook ME. Dietary conjugated linoleic acid Protects against end stage disease of lupus erythematosus in the NZB/W FI mouse. Immunopharmacol Immunotoxicol.2000.22:433- 449
68.Belury MA, Kempa SA. Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids. 1997.32:199-204
69.Moya CSY, Vanden HJP, Belury MA. Conjugated linoleic acid activates peroxisome proliferator activated receptor α and β subtypes but does not induce hepatic peroxisome proliferation in Sprague Dawley rats. Biochim Biophys Acta. 1999.1436:331-342
70.Moya CSY, Vanden HJP, Belury MA. Conjugated linoleic acd is a potent naturally occuring ligand and activator of PPARa Lipids Res. 1999.40:1426-1433
71.Banni S, Carta G, Angioni E, et al. Distribution of conjugated linoleic acid and metabolites in different lipid fractions in the rat liverlipid Res. 2001.42:1056-1061
72.Kavanaugh CJ, Liu KL, Belury MA. Effect of dietary conjugated linoleic acid on phorbol ester induced PGE2 production and hyperplasia in mouse epidermis. Nutr Cancer.1999.33:132-138
73.Li Y, Watkins BA. Conjugated linoleic acids alter bone fatty acid composition and reduce ex vivo prostaglandin E2 biosynthesis in rats fed n-6 or n-3 fatty acids. Lipids. 1998.33:417-425
74.IP C, Banni S, Angioni E, et al. Conjugated linoleic acid-enriched butter fat alters mammary gland morphogenesis and reduces cancer risk in rats. J Nutr. 1999.129.2135-2142
75.Eggert JM, Belury MA, Kempa SA, et al. Effects of conjugated linoleic acid on the belly firmness and fatty acid composition of genetically lean pigs Anim Sci. 2002.79:2866-2872
76.Sugano M, Tsujita A, Yamasaki M, et al.Conjugated linoleic acid modulates tissue levels of chemical mediator and immunoglobulins in rats Lipids. 1998.33:521-527
77. Liu KL, Belury MA. Conugated linoleic acid reduces arachidonic acid content and PGE2 synthesis in murine keratinocytes. Cancer Lett. 1998.124:1-8
78.Belury MA, Moya CSY, Liu KL.et al. Conjugated Iinoleic acid induces peroxisome proliferators associated enzyme expression and ornithine decarboxylase activity in mouse liver. Nutr Biochem. 1997.8:579-583
79.Bulgarella J, Patton D, Bull A. Modulation of Prostaglandin H synthase activity by conjugated Iinoleic acid (CLA) and specific CLA isomes.Lipids.2001. 36:407-512
80.Park Y, Storkson JM, Ntambi JM, et al. Inhibition of hepatic stearoyl-CoA desaturase activity by trans-10, cis-12 conjugated Iinoleic acid and its derivativesiBiochim Biophys Acta. 2000.1486:285-292
81.Sisk M, Hausman D, Martin R, et al. Dietary conjugated Iinoleic acid reduces adposity in lean but not obese Zucker rats. J Nutr. 2001.131:1668-1674
82.Lee KN, Pariza MW, Ntambi JM. Conjugated Iinoleic acid decreases hepatic stearoyl-CoA desaturase mRNA expression. Biochem. Biophys. Res Commum. 1998.248:817-821
83.Banni S, Angioni E, Casu V, et al. Decrease in Iinoleic acid metabolites as a potential mechanism in cancer risk reduction by conjugated Iinoleic acid. Carcinogenesis. 1999.20:1019-1024
84.Poulos SP, Sisk M, Hausman DB, et al. Pre- and postnatal dietary conjugated Iinoleic acid alters adipose development, body weigh gain and body composition in Sprague-Dawley rats. J Nutr. 2001. 131:2722-2731
85.Ntambi JM, Kim YC. Adipocyte differentiation and gene exprssion.J Nutr .2000.130:3122s-3126s
86.Belury MA, Moya CSY, Lu M, et al. Conjugated Iinoleic acid is an activator and ligand for peroxisome proliferators activated receptor gamma (PPAR gamma). Nutr Res. 2002.22 (7):817~824
87.Ryder JW, Portocarrero CP, Song XM, et al. Isomerspecific antidiabetic properties of conjugated Iinoleic acid. Diabetes.200l.6Q: 1149-1157
88.Sporn GI, Muller H, Kirchgessner M. Conjugated Iinoleic acid effects on circulating hormones, metabolites and lipoproteins, and its proportion in fasting serum and erythrocyte membranes of swine. Eur. J Nutr. 1999.38:271-277
89.Belury MA. Dietary conjugated Iinoleic acid in health: Physiological effects and mechanisms of action. Ann. Rev Nutr.2002.22:505-531
90.Chew BP, Wong TS, Shultz TD, et al. Effects of conjugated dienoic derivatives of Iinoleic acid and beta-carotene in modulating lymphocyte and macrophage function. Anticancer Res. 1997.17:1099- 1106
91. Wong MW, Chew BP, Wong TS, et al.Effects of dietary conjugated Iinoleic acid on lymphocyte function and growth of mammary tumors in mice. Anticancer Res. 1997.17:987-993
92.Hayek MG, Han SN, Wu DY, et al. Dietary conjugated Iinoleic acid influences the immune response of young and old C57BL/6NCrlBR mice. J Nutr. 1999.129:32-38
93.Bassaganya RJ, Hontecillas MR, Zimmerman DR. et al. Dietary conjugated Iinoleic acid modula- tes phenotype and effector functions of porcine CD8 (+) lymphocytes. J Nutr. 2001.131:2370- 2377
94.Bassaganya RJ, Hontecillas MR, Bregendahl K, et al. Effects of dietary conjugated Iinoleic acid in nursery pigs of dirty and clean environments on growth, empty body composition, and immune competence. J Anim Sci.2001.79:714-721
95.Bassaganya RJ, Hontecillas R, Zimmerman DR, et al. Long-term influence of lipid nutrition on the induction of CD8(+) responses to viral or bacterial antigens. Vaccine 2002.20 (9-10): 1435-1444
96.Cook, ME, Jerome DL, Pariza, MW. Broilers fed conjugated Iinoleic acid had enhanced bone ash. Poultry Science. 1997.76 (Supp.l):41-46
97.Watkins BA, Seifert MF. Conjugated Iinoleic acid and bone biology. J Am Coll Nutr. 2000.19:478S- 486S
98. Doyle E. Scientific forum explores CLA knowledge. INFORM. 1998. 9 (1): 69-73
99. Dugan MER, Aalhus JL, Schaefer AL, et al. The effect of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs. Can J Anim Sci. 1997. 77: 723-725
100. Ostrowska E, Muralitharan M, Cross RF, et al. Dietary conjugated linoleic acids increase lean tissue and decrease fat deposition in growing pigs. J Nut. 1999. 129: 2037-2042
101. Bee G. Dietary conjugated linoleic acid consumption during pregnancy and lactation influences growth and tissue composition in weaned pigs. J Nutr. 2000. 130: 2981-2989
102. Belury MA. Conjugated dienoic linoleate: a polyunsaturated fatty acid with enique chemoprotective properties. Nutr Rev. 1995. 53: 83-89
103. Banni S, Day BW, Evans RW, et al. Detection of conjugated diene isomers of linoleic acid in liver lipids of rats fed a cholinedevoid diet indicates that the diet does not cause lipoperoxidation. Nutr Biochem. 1995. 6: 281-89
104. Brown M, Evans M, Mcintosh M. Linoleic acid partially restores the triglyceride content of conjugated linoleic acid-treated cultures of 3T3-L1 preadipocytes. Nutr Biochem. 2001. 12: 381-87
105. Palmquist DL. Ruminal and endogenous synthesis of CLA in cows. Aust. J Dairy Technol. 2001. 56 (2): 134-137
106. Benjamin. AC, Lance. HB, Debra. AD, et al. The role of △9-desaturase in the production of cis-9, trans-11CLA J Nutr Biochem. 2001. 12: 622-630
107. Lin TY, Lin CW, Lee CH. Conjugated linoleic acid concentration as affected by lactic cultures and added linoleic acid. Food Chem. 1999. 67: 1-5
108. Griinari JM, Bauman DE. Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In Advances in Conjugated Linoleic Acid Research, Vol. 1. Champaign IL AOCS Press. 1999. pp. 180-200
109. Corl BA, Baumgard LH, Dwyer DA, et al. The role of △9-desaturase in the production of cis-9, trans-11 CLA. J Nutr Biochem. 2001. 12: 622-630
110. Cook ME, Pariza MW. Dietetic Food Containing Conjugated Linoleic Acid US Patent. 1998. 5, 760, 082
111. Krajca KE, Flow Process for Conjugated Unconjugated Unsaturation of Fatty Acids. US Patent. 1979. 4, 164, 505
112. Iwata T, Kamegai T, Sato Y, et al. Methed for Production Conjugated Linoleic Acid. US Patent. 1999. 5, 986, 116,
113. Saebo A, Skarie C, Jerome D. et al. Conjugated Linoleic Acid compositions and Methods of Making Same. US Patent. 2002. 6, 410, 761,
114.刘春奎.共轭亚油酸的生产方法.中国专利:2001.CN1289785A
115. Reaney MJ, Liu YD, Westcott ND, Method for Commercial Preparation of Conjugated Linoleic Acid. US Patent. 2002. 6, 420, 577,
116.李光友,刘发义,石红旗,微胶囊化共轭亚油酸.中国专利:2001.CNI287114A,
117. Seidel MC. Liquid Chromatography of High Purity Conjugated Fatty Acid. US Patent. 2002. 6, 410, 763,
118. Basu A, Bhaduri S. Process for the Manufacture of Conjugated Polyenoic Fatty Compounds. US Patent. 1985. 0, 160, 544,
119. Sleeter RT. Method of Conjugated Double Bonds in Drying Oils. US Patent. 1998. 5, 719, 301
120. Christie WW, Dobson G, Gunstone FD. Isomers in commercial samples of conjugated linoleic acid. Lipids. 1997. 32: 1231-1235
121. Kim YJ, Liu RH, Bond DR, et al. Effect of linoleic acid concentration on conjugated linoleic acid production by Butyrivibrio fibrisolvens A38. Appl Environ Microbiol. 2000. 66 (12): 5226-5230
122. Kim YJ, Liu RH, Rychlik JL, et al. The enrichment of a ruminal bacterium (Megasphaera elsdenii YJ-4) that produces the trans-10, cis-12 isomer of conjugated linoleic acid. J Appl Microbiol. 2002. 92: 976-982
123. Jiang J, Bjorck L, Fonden R. Production of conjugated linoleic acid by dairy starter cultures. J Appl Microbiol. 1998. 85: 95-102
124. Rainio A, Vahvaselka M, Suomalainen T, et al. Reduction of linoleic acid inhibition in production of conjugated linoleic acid by Propionibacterium freudenreichii ssp shermanii. Can J Microbiol. 2001. 47: 735-740
125. Rainio A, Vahvaselka M, Suomalainen T, et al. Production of conjugated linoleic acid by Propionibacterium freudenreichii ssp shermanii. Lait. 2002. 82 (1): 91-101
126. Lin TY. Conjugated linoleic acid concentration as affected by lactic cultures and additives. Food Chem. 2000. 69: 27-31
127. Lin TY, Lin CW, Wang YJ. Linoleic acid isomerase activity in enzyme extracts from Lactobacillus acidophilus and Propionibacterium freudenreichii ssp shermanii. J Food Sci. 2002. 67(4): 1502-505
128. Pariza, MW. Yang XY. Method of producing conjugated fatty acids. US Patent. 2000. 6, 060, 304.
129. Kim YJ, Liu RH, Increase of conjugated linoleic acid content in milk by fermentation with lactic acid bacteria. J Food Sci. 2002. 67 (5): 1731-1737
130. Ham JS, In YM, Jeong SG, et al. Screening of conjugated linoleic acid producing lactic acid bacteria from fecal samples of healthy babies. Asian-Australasian J Anim Sci. 2002. 15 (7):1031-1035
131. Ogawa J, Matsumura K, Kishino S, et al. Conjugated linoleic acid accumulation via 10-hydroxy-12 octadecaenoic acid during microaerobic transformation of linoleic acid by Lactobacillus acidophilus. Appl Environ Microbiol. 2001. 67 (3): 1246-1252
132. Kishino S, Ogawa J, Omura Y, et al. Conjugated linoleic acid production from linoleic acid by lactic acid bacteria. J Am Oil Chem Soc. 2002. 79 (2): 159-163
133.陈忠周,李艳梅,赵刚等.共轭亚油酸的性质及合成.中国油脂.2000.25(5):41—45
134. Pariza, MW, Yang XY, Method of producing conjugated fatty acids. US Patent. 1999. 5, 856, 149
135.邵群,边际,马丽等.乳酸菌发酵生产共轭亚油酸条件的研究.山东师范大学学报(自然科学版)2001,16(4):443-446
136.曹键,魏明,曾实等.一株嗜酸乳杆菌突变株转化亚油酸为共轭亚油酸条件的研究.食品科学2003,24(9):76-79
137.熊向峰,陈超银,赵声兰等.天然植物油碱异构化合成共轭亚油酸的研究.中国油脂2003,28(4):39-41
138.张亚刚,樊莉,文彬等.紫外可见分光光度法在共轭亚油酸定量分析中的应用.新疆石油学院学报2002,14(2):59—64
139.张亚刚,樊莉,马莉等.用紫外可见分光光度法测定共轭亚油酸的含量.中国科学院研究生院学报 2002,19(4):436-442
140.吴冀华,裘爱泳.共轭亚油酸的分析.中国油脂,2002,27(2):12-13
141. Kishino S, Ogawa J, Ando A, et al. Structural Analysis of Conjugated Linoleic Acid Produced by Lactobacillus plantarum, and Factors Affecting Isomer Production. Biosci Biotechnol Biochem. 2003. 67 (1): 179-182
142. Fogerty AC, Ford GL, Svoronos D. Octadeca-9, 11-dienoic acid in food stuffs and in the lipids of human blood and breast milk. Nutr Rep Int. 1988. 38: 937-944
143.凌代文,东秀珠.乳酸细菌分类鉴定及实验方法.北京:中国轻工业出版社,1999,3:85-129
144.钱存柔,黄仪秀.微生物学实验教程.北京:北京大学出版社,1999
145. Wijtzes T, Bruggeman M R, Nout M J. et al. A computerised system for the identification of lactic acid bacteria. International Food Microbiology. 1997. 38 (1): 65-70
146.杜连祥等.工业微生物实验技术.天津:天津科技出版社,1992
147.姚汝华.微生物工程工艺原理.广州:华南理工大学出版社,1996
148.戚以政,汪叔雄.生化反应动力学与反应器.北京:化学工业出版社,1999,第2版
149.天津轻工业学院等编.工业发酵分析.北京:轻工业出版社,1980
150. Bessa RJB, Santos S J, Ribeiro JMR, et al. Reticulorumen biohydrogenation and the enrichment of ruminant edible products with linoleic acid conjugated isomers. Livestock Prod Sci. 2000. 63: 201-211
151.中华人民共和国国家质量技术监督局.GB/T17376-1998.中华人民共和国国家标准—动植物油脂脂肪酸甲酯制备.北京:中国标准出版社,1998-05-08
152. Roach JAG, Mossoba MM, Yurawecz MP, et al. Chromatographic separation and identification of conjugated linoleic acid isomers. Analytica Chimica Acta. 2002. 465: 207-226
153.中华人民共和国国家质量技术监督局.GB/T 17377-1998动植物油脂 脂肪酸甲酯的气相色谱分析.北京:中国标准出版社,1998-05-08
154. Kishino S, Ogawa J, Ando A, et al. Conjugated alpha-linolenic acid production from a-linolenic linolenic acid by Lactobacillus plantarum AKU 1009a. European J Lipid Sci Technol. 2003. 105 (10): 572-577
155.吴冀华,裘爱泳.气相色谱法分析共轭亚油酸异构体.中国油脂,2002,27(1):65-67
156. Rosson RA, Grund AD, et al. Linoleate Isomerase. World Patent. 2001. 0100846,
157.汪家政,范明.蛋白质技术手册.北京:科学出版社,2001,189-210
158.蔡武城,李碧羽,李玉民.生物化学实验技术教程.上海:复旦大学出版社,1983,1-8
159.曹键,魏明,曾实等.一株嗜酸乳杆菌突变株亚油酸异构酶的纯化及性质.食品与发酵工业 2004,30(2):48-52
160. Vant VP, Dekker JM, Larmars JWJ, et al. Consumption of fermented milk products and breast cancer: A case-control study in the Netherlands. Cancer Res. 1989. 49: 4020-4023
161. Mccarthy AC, La E, Conti CJ. et al. Effect of spray-dried yogurt and lactic acid bacteria on the initiation and promotion stages of chemically induced skin carcinogenesis in mice. Nutr Cancer. 1997. 27: 231-237
162. Kimura KA, Mccartneym A, Mcconnel M, et al. Analysis of fecal populations of bifidobacteria and latobacilli and investigation of the immunological response of their human host to the predominant strains. Appl Environ Microbiol. 1997. 63: 3394-3398
163. Xue L, Lipkin M, Newmark H, et al. Influence of dietary calcium and vitamin D on diet-induced epithelial cell hyperproliferation in mice. J Nat Cancer Inst. 1999. 91: 176—181
164. Cassand P, LevEque F, Decoudu S, et al. Relationship between vitamin A dietary intake and mutagenic activity of some chemical carcinogens, Food Addit Contam. 1990. 7:S58-S59
165. Dhiman TR, Helmink ED, McMahon DJ, et al. Conjugated linoleic acid content of milk and cheese from cows fed extruded oilseeds. J Dairy Sci. 1999, 82: 412-419
166. Kelly ML, Kolver ES, Bauman DE, et al. Effect of intake of pasture on concentrations of conjugated linoleic acid in milk of lactating cows. J Dairy Sci. 1998. 81: 1630-1636
167. McGuire MA, Duckett SK, Andrae G, et al. Effect of high-oil corn on content of conjugated linoleic acid (CLA) in beef. J Dairy Sci. 1998. 81: 301-309
168.杜连祥,赵征,王敏第.乳酸菌及其发酵制品生产技术.天津:天津科学技术出版社,1999,91-95
169.金世林编.乳与乳制品生产.北京:中国轻工业出版社,1990
170. Ando A, Ogawa J, Kishino S, et al. CLA production from ricinoleic acid by lactic acid bacteria. J. Am. Oil Chem. Soc. 2003. 80 (9): 889-894