日粮能量对朗德鹅和溆浦鹅生长性能及肌肉生成抑制因子（MSTN）基因表达影响的研究
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摘要
朗德鹅和溆浦鹅是国内主要的鹅肥肝品种。在肥肝鹅产业化开发过程中,品系培育是其中的一个重要方面。大多数研究表明,日粮ME水平对早期鹅增重的影响比蛋白质水平影响大,但少有研究者就能量一个因素对肥肝鹅品系的早期生长发育进行特定研究。本课题通过改变日粮代谢能,结合朗德鹅和溆浦鹅早期生长情况、关键日龄屠体指标、相关血液生化指标和血清激素水平和肌肉生成抑制因子(MSTN)基因表达量等因素,探索朗德鹅和溆浦鹅早期生长发育规律、超饲养前期所需要的适宜ME营养需要和涉及早期生长发育的相关规律。试验结果分别摘要如下:
试验1 0～70d阶段能量对朗德鹅公鹅生长发育影响的研究
目的:研究不同日粮能量水平对朗德鹅公鹅超饲养前期(0～70d)生长性能指标、屠体性能、体尺指标、血液生化指标、激素水平和肌肉成分的影响研究。方法:本试验用90只0日龄初生重大致相同的朗德鹅公鹅为试验对象,通过添加豆油来设计不高中低三种不同代谢能水平(0～3w:13.38MJ/kg、12.13MJ/kg和10.87MJ/kg;4～10w:13.81MJ/kg、12.55MJ/kg和11.30MJ/kg)的日粮,试验鹅随机分为试验Ⅰ组,试验Ⅱ组,试验Ⅲ组,每组30只,分栏饲养70天。每周进行称重和体尺测定;于21d、70d进行屠宰试验,屠宰前采集血液进行血清参数和血清激素水平检测;屠宰后测定肌肉成分。结果:(1)适宜朗德鹅公鹅能量水平:0～21d为12.13MJ/kg、22～70d为12.55MJ/Kg;(2)日粮能量对于朗德鹅公鹅屠宰率、全净膛率、半净膛率、腿肌率的影响差异均不显著(p＞0.05);(3)朗德鹅公鹅肌肉中的蛋白质含量超过21%;胸肌和腿肌总脂含量都是随着日粮能量浓度提高而提高,Ⅰ组胸肌和腿肌总脂含量比Ⅲ组分别高24.22%和34.73%;胸肌中各项化学成分含量高于腿肌;(4)21d时,朗德鹅公鹅血清中TG、LDL、HLDL差异不显著(p＞0.05);70d时,朗德鹅公鹅血清中AST、LDL和HLDL的含量差异不显著(p＞0.05);(5)21d血清IGF—Ⅰ测定值(♂Ⅲ除外)均高于70d时各组测定值;且ME水平对21d时血清IGF—Ⅰ水平影响差异显著(p＜0.05);21d血清GH测定值随日粮能量的升高而升高,70d时,公鹅各组GH差异不显著(p＞0.05)。
试验2 0～70d阶段能量对朗德鹅母鹅生长发育影响的研究
目的:研究不同日粮能量水平对朗德鹅母鹅超饲养前期(0～70d)生长性能指标、屠体性能、体尺指标、血液生化指标、激素水平和肌肉成分的影响研究。方法:同朗德鹅公鹅。结果:(1)适宜朗德鹅母鹅能量水平:0～21d为13.38MJ/kg、22～70d为13.81MJ/Kg;(2)各组母鹅腿肌中干物质含量分别要高于同组的胸肌干物质含量约4.58%、0.21%和10.29%;胸肌中总脂含量分别要高于同组的腿肌总脂含量约1.53%、6.91%和1.88%;(3)21d时,朗德鹅母鹅TG、LDL、HLDL差异不显著(p＞0.05);70d时,朗德鹅母鹅血清中AST、LDL、TC、AKP和HLDL的含量差异不显著(p＞0.05)。(4)21d血清IGF—Ⅰ测定值均高于70d测定值;21d时,母鹅IGF—Ⅰ各组差异不显著(p＞0.05),70d差异显著(p＜0.05)。21d时,母鹅GH随日粮能量的提高而提高。
试验3 0～70d阶段能量对溆浦鹅生长发育影响的研究
目的:研究不同日粮能量水平对溆浦鹅超饲养前期(0～70d)生长性能指标、屠体性能、体尺指标、血液生化指标、激素水平和肌肉成分的影响研究。方法:同朗德鹅公鹅。结果:(1)适宜溆浦鹅能量水平:0～21d能量水平为13.38MJ/kg、22～70d能量水平为13.81MJ/Kg。(2)采食中等能量的溆浦鹅Ⅱ组在屠宰率、全净膛率、半净膛率、腹脂率和胸肌率五个指标上和其余两组差异显著(p＜0.05);对于皮下脂肪厚度和肌间脂肪厚度两个指标上溆浦鹅Ⅰ、Ⅱ组和Ⅲ组之间差异显著(p＜0.05);Ⅱ组的腹脂重和腹脂率高于其余两组,差异显著(p＜0.05)。(3)采食不同能量日粮溆浦鹅胸肌和腿肌蛋白质含量差异不显著(p＞0.05);Ⅰ组胸肌中总脂含量分别比Ⅱ、Ⅲ组胸肌总脂含量高4.46%和8.66%;Ⅰ组腿肌中总脂含量分别比Ⅱ、Ⅲ组腿肌总脂含量高4.99%和11.63%。(4)21d和70d时,溆溆浦鹅血清IGF—Ⅰ测定值差异不显著(p＞0.05);21d时血清GH测定值低于70d时测定值。
试验1—3结论:
(1)朗德鹅公鹅、朗德鹅母鹅和溆浦鹅早期适宜ME水平:0～21d分别为12.13MJ/kg、13.38MJ/kg和13.38MJ/kg;22～70d分别为12.55MJ/kg、13.81MJ/kg和13.81MJ/kg;(2)本试验中,日粮能量水平可以影响鹅的体尺发育;(3)ME水平显著影响朗德鹅部分屠宰指标(公鹅屠体重、全净膛重、半净膛重、胸肌重、腿肌重和腹脂重);日粮能量水平对朗德鹅屠宰率、全净膛率、半净膛率、腿肌率的影响差异均不显著;(4)ME水平极显著影响溆浦鹅的屠宰率、全净膛率、半净膛率、腹脂率和胸肌率等五个指标;(5)朗德鹅肌肉粗脂肪含量高于溆浦鹅肌肉粗脂肪含量;同等能量水平下朗德鹅公鹅粗蛋白质、粗脂肪和粗灰分含量高于朗德鹅母鹅;ME水平会影响肌肉化学组成;(6)GH水平随ME提高而提高;血清IGF—Ⅰ水平和日粮能量水平无相关性。
试验4朗德鹅、溆浦鹅肌肉生成抑制因子(MSTN)基因的克隆和能量对其表达量影响的研究
目的:采用反转录—聚合酶链反应(RT—PCR)技术,克隆朗德鹅、溆浦鹅肌肉生成抑制因子(MSTN)基因,研究MSTN基因表达和日粮能量及其部分血清激素含量的相互关系,了解MSTN的功能,为水禽分子营养和分子育种提供基础资料。方法:从朗德鹅、溆浦鹅的腿肌中抽提总RNA,用两步法RT—PCR扩增出MSTN基因的cDNA编码序列,以pGEM—T Vector为载体,将该片段克隆到大肠杆菌(Escherichia coli)中。通过筛选阳性克隆,双酶切鉴定后测序;以MSTN基因的克隆为基础,以β—actin为内标,构建优化的半定量RT—PCR方法,研究三种不同能量对朗德鹅公鹅、朗德鹅母鹅和溆浦鹅在21d和70d两个时期肌肉组织MSTN基因表达的差异;同时用放免法测定21、70d的血清GH、IGF—Ⅰ浓度。结果:(1)克隆到朗德鹅、溆浦鹅MSTN基因cDNA的部分序列,其片段大小为1128bp,编码375个氨基酸组成的多肽,与已发表的鸡、鸭、鹅的MSTN核苷酸相似性分别为94%、94%、99%;氨基酸的相似性分别为98%、97%、98%;(2)21d时,朗德鹅公鹅、母鹅和溆浦鹅MSTN表达量差异不显著;(3)70d时朗德鹅公鹅MSTN的表达量情况为Ⅰ＞Ⅲ＞Ⅱ,朗德鹅母鹅MSTN的表达量情况为Ⅲ＞Ⅱ＞Ⅰ,溆浦鹅MSTN的表达量情况为Ⅲ＞Ⅱ＞Ⅰ;(4)日粮能量对21d后朗德鹅、溆浦鹅MSTN基因的表达有影响,MSTN基因的表达量和血清IGF—Ⅰ的变化趋势基本一致;与血清GH含量无相关性,GH和能量的高低呈正相关。结论:日粮能量对21d后朗德鹅、溆浦鹅MSTN基因的表达有影响,且MSTN基因表达量和血清IGF—Ⅰ具有相关性,和血清GH无相关性。
Landes geese and Xupu geese are the main kinds domestic fatty liver geese in China currently.Breeding fatty liver geese is one of the most important aspects in the development process of geese industrialization.Most researchers consider ME levels exert more greater influence on earlier period geese gain than protein level,but up to the present,there are no researchers who research a energy factor to the geese kind specifically.The earlier growth performance rule of landes geese and Xupu geese,the standard of feasible ME nutrition needing beyond earlier period and over-feeding in this two kinds of geese were researched connected with the earlier period growth performace, the carcass traits,MSTN gene expression,IGF-Ⅰand GH level in serum which closely related with growth development.The results summarized as follows:
Experiment 1 Effect of Energy on Male Landes Geese Growth Performance on 0-70 Days Stage
Objective:To research effect of different ME level on growth performance,the carcass trait,body measurement,serum Parameters,hormone level and muscle composition of male landes geese in pre-overfeeding(0-70 days).Methods:90 0-day-old Landes geese about similar body weight each were used to study the effects of diets containing different metabolic energy levels(0～21d:13.38MJ/kg,12.13MJ/kg and 10.87MJ/k. 22～70d:13.81MJ/kg、12.55MJ/kg and 11.30MJ/kg) through adding soybean oil with equal CP%on bird growth performance,dressed performance,the carcass traits,body measurement,partial serum parameters and IGF-Ⅰ,GH levels in serum.Results:(1) The best ME to male geese is 12.13MJ/kg(0-21d) and 12.55MJ/kg(22-70d) in order to obtaining the best growth performance.(2) Using soybean oil as energy adding,the dressed carcass percentage,eviscerated carcass weight rate,semi-eviscerated carcass weight rate,leg muscle rate of male landes geese were showed no significantly differences(p＞0.05) between these treatments.(3) Protein content in landes male geese's muscle was all beyond 21%whatever fed with any kinds of energy.Total fattiness content of chest and leg muscle increased with daily food energy concentration,in this experiment that of♂Ⅰwhich food intake energy was the highest respectively higher 24.22%and 34.73%than that of♀Ⅲwhich food intake energy was the lowest;each composition content in chest muscle was larger than that in leg muscle.(4)TG,LDL and HLDL in serum were showed no significantly differences(p＞0.05) between these treatments at 21d; at 70d,the AST,LDL and HLDL in serum were showed no significantly differences (p＞0.05).(5) IGF—Ⅰlevel in serum at 21d was higher than that at 70d except♂Ⅲ.IGF-Ⅰlevel showed significantly differences(p＜0.05) at 21d.Male landes geese's GH levels in serum at 21d was increased with ME,at 70d,that difference of all groups was unremarkable(p＞0.05).
Experiment 2 Effect of Energy on female Landes Geese Growth Performance on 0-70 Days Stage
Objective:To research effect of different ME levels on growth performance,the carcass trait,body measurement,serum Parameters,hormone level and muscle composition of female landes geese in pre-overfeeding(0-70 days).Methods:According to the design of experiment 1 above.Results:(1) The best ME to female geese is 13.38MJ/kg (0-21d) and 13.81MJ/kg(22-70d) in order to obtaining the best growth performance.(2) Muscle basic composition analysis of 70d female landes geese showed the dry material content of leg muscle was higher than chest muscle about 4.58%,0.21%and 10.29%in the same treatment,total fattiness content of leg muscle was higher than chest muscle about 1.53%,6.91%and 1.88%in the same treatment.(3) Using soybean oil as energy adding,the TG,LDL and HLDL in serum were showed no significantly differences (p＞0.05) between these treatments at 21d.At 70d,the AST,LDL TC AKP and HLDL in serum were showed no significantly differences(p＞0.05).(4) IGF—Ⅰlevel in serum at 21d was higher than that at 70d each treatment.IGF-Ⅰlevel showed no significantly differences(p＞0.05) at 21d,while it showed significantly differences(p＜0.05) at 70d,; female landes geese's serum GH level at 21d was increased with ME.
Experiment 3 Effect of Energy on Xupu Geese Growth Performance on 0-70 Days Stage
Objective:To research effect of different ME level on growth performance,the carcass trait,body measurement,serum Parameters,hormone level and muscle composition of Xupu geese in pre-overfeeding(0-70 days).Methods:According to the design of experiment 1 above.Results:(1) The best ME to Xupu geese is 13.38MJ/kg (0-21d) and 13.81MJ/kg(22-70d) in order to obtaining the best growth performance.(2) The carcass rate,eviscerated carcass rate,semi- eviscerated carcass rate,abdomen fat ratio and chest muscle rate were significant(p＜0.05) compared to the second group and the other two groups.As for subcutaneous fat layer thickness and intermuscle fat thickness,diversity were significant(p＜0.05) amone three groups.Abdomen fat weight and abdomen fat ratio of the second group were high than other two groups(p＜0.05).(3) Protein content of breast and leg muscle of Xupu geese which intake different energy diety has not significant diversity among three groups.Total lipids of chest muscle of the first group higher 4.46%,8.66%than the second and the third group,respectivelly.Total lipids of leg muscle of the first group higher 4.99%,11.63%than the second and the third group respectivelly.(4)There have no significant differences(p＞0.05) of IGF-Ⅰlevels at 21d or 70d.GH levels in serum of Xupu geese at 21d were low than its at 70d. Conclusions of Experiment 1-3:(1) The best ME to male geese is 12.13MJ/kg(0-21d) and 12.55MJ/kg(22-70d) in order to obtaining the best growth performance.The best ME to female geese is 13.38MJ/kg(0-21d) and 13.81MJ/kg(22-70d) in order to obtaining the best growth performance.The best ME to Xupu geese is 13.38MJ/kg(0-21d) and 13.81MJ/kg(22-70d) in order to obtaining the best growth performance.(2)In this experiment,ME could influence body sizes in limited range;(3) ME influence some parameters which have closely relation with growth performance like slaught weight of male landes geese,eviscerated carcass weight,semi-eviscerated carcass weight,breast muscle weight,leg muscle weight and abdominal fat weight of landes geese were showed significantly differences(p＜0.05) between these treatments;but dressed carcass percentage,eviscerated carcass weight rate,semi-eviscerated carcass weight rate,leg muscle rate of landes geese were showed no significantly differences(p＞0.05) which effected by ME level.(4)ME levels influenced Xupu geese's dressed carcass percentage, eviscerated carcass weight,semi-eviscerated carcass weight rate,abdominal fat rate and breast muscle rate significantly(p＜0.05).(5)Muscle lipid content of landes geese was higher than it of Xupu geese.CP,total lipid and DM content of male landes geese were higher than those of female landes geese,and the chemical constitution of geese muscles would changed with ME levels.(6) GH levels in serum were influenced by ME levels, while there was a positive relationship between them;IGF-Ⅰlevels in serum has no relationship with ME levels.
Experiment 4 The Research of MSTN Gene Clone and energy Influence on MSTN Gene Expression in Landes Geese and Xupu Geese
Objective:Cloning of MSTN gene of Landes geese and Xupu geese the relationship was researched between MSTN gene expression and different energy diets or IGF-Ⅰ,GH in serum in order to know the function of MSTN gene and provide information for molecular nutriology and breeding of waterfowl.Methods:Genome RNA was extracted from muscle of Landes geese and Xupu geese,MSTN gene mRNA was amplified by using RT-PCR,the PCR product was cloned into pGEM-T vector.The MSTN gene was isolated and sequenced from the positive clones screened Based on the MSTN gene clone, usingβ-actin as inner control,constructing an optimal semi-quantitative RT-PCR method, the difference of MSTN gene expression at 21 and 70 days by feeding different energy diets,at one time we detected IGF-Ⅰand GH in serum.Results:(1) The unique DNA fragment about 1128bp in length was obtained.Sequence analysis revealed that it has a length of 1128 nucleotides which encodes a 375 amino peptide.Compared with homologous sequence of human,rat,duck and goose,it displayed a fairly high degree conservation.The homologue of the nucleotide sequence are 94%,94%,99%respectively, the homologue of the amino acid sequence are 98%,97%,98%sespectively.(2) At 21 days,the expressions of MSTN in Landes geese or Xupu geese were showed no significantly differences.(3) At 70 days,the expression of MSTN in male wasⅠ＞Ⅲ＞Ⅱby 70d,to female wasⅢ＞Ⅱ＞Ⅰby 70d,to Xupu geese wasⅢ＞Ⅱ＞Ⅰby 70d.(4) In 21～70 days period,the expression of MSTN was changed with IGF-Ⅰin serum,it was not associated with GH in serum.GH was significant correlations with energy.Conclusions: The different energy influenced MSTN gene expression of landes geese and Xupu geese; in this period,the MSTN gene expression was associated with IGF-Ⅰin serum,but it was not associated with GH in serum.
试验1 0～70d阶段能量对朗德鹅公鹅生长发育影响的研究
目的:研究不同日粮能量水平对朗德鹅公鹅超饲养前期(0～70d)生长性能指标、屠体性能、体尺指标、血液生化指标、激素水平和肌肉成分的影响研究。方法:本试验用90只0日龄初生重大致相同的朗德鹅公鹅为试验对象,通过添加豆油来设计不高中低三种不同代谢能水平(0～3w:13.38MJ/kg、12.13MJ/kg和10.87MJ/kg;4～10w:13.81MJ/kg、12.55MJ/kg和11.30MJ/kg)的日粮,试验鹅随机分为试验Ⅰ组,试验Ⅱ组,试验Ⅲ组,每组30只,分栏饲养70天。每周进行称重和体尺测定;于21d、70d进行屠宰试验,屠宰前采集血液进行血清参数和血清激素水平检测;屠宰后测定肌肉成分。结果:(1)适宜朗德鹅公鹅能量水平:0～21d为12.13MJ/kg、22～70d为12.55MJ/Kg;(2)日粮能量对于朗德鹅公鹅屠宰率、全净膛率、半净膛率、腿肌率的影响差异均不显著(p＞0.05);(3)朗德鹅公鹅肌肉中的蛋白质含量超过21%;胸肌和腿肌总脂含量都是随着日粮能量浓度提高而提高,Ⅰ组胸肌和腿肌总脂含量比Ⅲ组分别高24.22%和34.73%;胸肌中各项化学成分含量高于腿肌;(4)21d时,朗德鹅公鹅血清中TG、LDL、HLDL差异不显著(p＞0.05);70d时,朗德鹅公鹅血清中AST、LDL和HLDL的含量差异不显著(p＞0.05);(5)21d血清IGF—Ⅰ测定值(♂Ⅲ除外)均高于70d时各组测定值;且ME水平对21d时血清IGF—Ⅰ水平影响差异显著(p＜0.05);21d血清GH测定值随日粮能量的升高而升高,70d时,公鹅各组GH差异不显著(p＞0.05)。
试验2 0～70d阶段能量对朗德鹅母鹅生长发育影响的研究
目的:研究不同日粮能量水平对朗德鹅母鹅超饲养前期(0～70d)生长性能指标、屠体性能、体尺指标、血液生化指标、激素水平和肌肉成分的影响研究。方法:同朗德鹅公鹅。结果:(1)适宜朗德鹅母鹅能量水平:0～21d为13.38MJ/kg、22～70d为13.81MJ/Kg;(2)各组母鹅腿肌中干物质含量分别要高于同组的胸肌干物质含量约4.58%、0.21%和10.29%;胸肌中总脂含量分别要高于同组的腿肌总脂含量约1.53%、6.91%和1.88%;(3)21d时,朗德鹅母鹅TG、LDL、HLDL差异不显著(p＞0.05);70d时,朗德鹅母鹅血清中AST、LDL、TC、AKP和HLDL的含量差异不显著(p＞0.05)。(4)21d血清IGF—Ⅰ测定值均高于70d测定值;21d时,母鹅IGF—Ⅰ各组差异不显著(p＞0.05),70d差异显著(p＜0.05)。21d时,母鹅GH随日粮能量的提高而提高。
试验3 0～70d阶段能量对溆浦鹅生长发育影响的研究
目的:研究不同日粮能量水平对溆浦鹅超饲养前期(0～70d)生长性能指标、屠体性能、体尺指标、血液生化指标、激素水平和肌肉成分的影响研究。方法:同朗德鹅公鹅。结果:(1)适宜溆浦鹅能量水平:0～21d能量水平为13.38MJ/kg、22～70d能量水平为13.81MJ/Kg。(2)采食中等能量的溆浦鹅Ⅱ组在屠宰率、全净膛率、半净膛率、腹脂率和胸肌率五个指标上和其余两组差异显著(p＜0.05);对于皮下脂肪厚度和肌间脂肪厚度两个指标上溆浦鹅Ⅰ、Ⅱ组和Ⅲ组之间差异显著(p＜0.05);Ⅱ组的腹脂重和腹脂率高于其余两组,差异显著(p＜0.05)。(3)采食不同能量日粮溆浦鹅胸肌和腿肌蛋白质含量差异不显著(p＞0.05);Ⅰ组胸肌中总脂含量分别比Ⅱ、Ⅲ组胸肌总脂含量高4.46%和8.66%;Ⅰ组腿肌中总脂含量分别比Ⅱ、Ⅲ组腿肌总脂含量高4.99%和11.63%。(4)21d和70d时,溆溆浦鹅血清IGF—Ⅰ测定值差异不显著(p＞0.05);21d时血清GH测定值低于70d时测定值。
试验1—3结论:
(1)朗德鹅公鹅、朗德鹅母鹅和溆浦鹅早期适宜ME水平:0～21d分别为12.13MJ/kg、13.38MJ/kg和13.38MJ/kg;22～70d分别为12.55MJ/kg、13.81MJ/kg和13.81MJ/kg;(2)本试验中,日粮能量水平可以影响鹅的体尺发育;(3)ME水平显著影响朗德鹅部分屠宰指标(公鹅屠体重、全净膛重、半净膛重、胸肌重、腿肌重和腹脂重);日粮能量水平对朗德鹅屠宰率、全净膛率、半净膛率、腿肌率的影响差异均不显著;(4)ME水平极显著影响溆浦鹅的屠宰率、全净膛率、半净膛率、腹脂率和胸肌率等五个指标;(5)朗德鹅肌肉粗脂肪含量高于溆浦鹅肌肉粗脂肪含量;同等能量水平下朗德鹅公鹅粗蛋白质、粗脂肪和粗灰分含量高于朗德鹅母鹅;ME水平会影响肌肉化学组成;(6)GH水平随ME提高而提高;血清IGF—Ⅰ水平和日粮能量水平无相关性。
试验4朗德鹅、溆浦鹅肌肉生成抑制因子(MSTN)基因的克隆和能量对其表达量影响的研究
目的:采用反转录—聚合酶链反应(RT—PCR)技术,克隆朗德鹅、溆浦鹅肌肉生成抑制因子(MSTN)基因,研究MSTN基因表达和日粮能量及其部分血清激素含量的相互关系,了解MSTN的功能,为水禽分子营养和分子育种提供基础资料。方法:从朗德鹅、溆浦鹅的腿肌中抽提总RNA,用两步法RT—PCR扩增出MSTN基因的cDNA编码序列,以pGEM—T Vector为载体,将该片段克隆到大肠杆菌(Escherichia coli)中。通过筛选阳性克隆,双酶切鉴定后测序;以MSTN基因的克隆为基础,以β—actin为内标,构建优化的半定量RT—PCR方法,研究三种不同能量对朗德鹅公鹅、朗德鹅母鹅和溆浦鹅在21d和70d两个时期肌肉组织MSTN基因表达的差异;同时用放免法测定21、70d的血清GH、IGF—Ⅰ浓度。结果:(1)克隆到朗德鹅、溆浦鹅MSTN基因cDNA的部分序列,其片段大小为1128bp,编码375个氨基酸组成的多肽,与已发表的鸡、鸭、鹅的MSTN核苷酸相似性分别为94%、94%、99%;氨基酸的相似性分别为98%、97%、98%;(2)21d时,朗德鹅公鹅、母鹅和溆浦鹅MSTN表达量差异不显著;(3)70d时朗德鹅公鹅MSTN的表达量情况为Ⅰ＞Ⅲ＞Ⅱ,朗德鹅母鹅MSTN的表达量情况为Ⅲ＞Ⅱ＞Ⅰ,溆浦鹅MSTN的表达量情况为Ⅲ＞Ⅱ＞Ⅰ;(4)日粮能量对21d后朗德鹅、溆浦鹅MSTN基因的表达有影响,MSTN基因的表达量和血清IGF—Ⅰ的变化趋势基本一致;与血清GH含量无相关性,GH和能量的高低呈正相关。结论:日粮能量对21d后朗德鹅、溆浦鹅MSTN基因的表达有影响,且MSTN基因表达量和血清IGF—Ⅰ具有相关性,和血清GH无相关性。
Landes geese and Xupu geese are the main kinds domestic fatty liver geese in China currently.Breeding fatty liver geese is one of the most important aspects in the development process of geese industrialization.Most researchers consider ME levels exert more greater influence on earlier period geese gain than protein level,but up to the present,there are no researchers who research a energy factor to the geese kind specifically.The earlier growth performance rule of landes geese and Xupu geese,the standard of feasible ME nutrition needing beyond earlier period and over-feeding in this two kinds of geese were researched connected with the earlier period growth performace, the carcass traits,MSTN gene expression,IGF-Ⅰand GH level in serum which closely related with growth development.The results summarized as follows:
Experiment 1 Effect of Energy on Male Landes Geese Growth Performance on 0-70 Days Stage
Objective:To research effect of different ME level on growth performance,the carcass trait,body measurement,serum Parameters,hormone level and muscle composition of male landes geese in pre-overfeeding(0-70 days).Methods:90 0-day-old Landes geese about similar body weight each were used to study the effects of diets containing different metabolic energy levels(0～21d:13.38MJ/kg,12.13MJ/kg and 10.87MJ/k. 22～70d:13.81MJ/kg、12.55MJ/kg and 11.30MJ/kg) through adding soybean oil with equal CP%on bird growth performance,dressed performance,the carcass traits,body measurement,partial serum parameters and IGF-Ⅰ,GH levels in serum.Results:(1) The best ME to male geese is 12.13MJ/kg(0-21d) and 12.55MJ/kg(22-70d) in order to obtaining the best growth performance.(2) Using soybean oil as energy adding,the dressed carcass percentage,eviscerated carcass weight rate,semi-eviscerated carcass weight rate,leg muscle rate of male landes geese were showed no significantly differences(p＞0.05) between these treatments.(3) Protein content in landes male geese's muscle was all beyond 21%whatever fed with any kinds of energy.Total fattiness content of chest and leg muscle increased with daily food energy concentration,in this experiment that of♂Ⅰwhich food intake energy was the highest respectively higher 24.22%and 34.73%than that of♀Ⅲwhich food intake energy was the lowest;each composition content in chest muscle was larger than that in leg muscle.(4)TG,LDL and HLDL in serum were showed no significantly differences(p＞0.05) between these treatments at 21d; at 70d,the AST,LDL and HLDL in serum were showed no significantly differences (p＞0.05).(5) IGF—Ⅰlevel in serum at 21d was higher than that at 70d except♂Ⅲ.IGF-Ⅰlevel showed significantly differences(p＜0.05) at 21d.Male landes geese's GH levels in serum at 21d was increased with ME,at 70d,that difference of all groups was unremarkable(p＞0.05).
Experiment 2 Effect of Energy on female Landes Geese Growth Performance on 0-70 Days Stage
Objective:To research effect of different ME levels on growth performance,the carcass trait,body measurement,serum Parameters,hormone level and muscle composition of female landes geese in pre-overfeeding(0-70 days).Methods:According to the design of experiment 1 above.Results:(1) The best ME to female geese is 13.38MJ/kg (0-21d) and 13.81MJ/kg(22-70d) in order to obtaining the best growth performance.(2) Muscle basic composition analysis of 70d female landes geese showed the dry material content of leg muscle was higher than chest muscle about 4.58%,0.21%and 10.29%in the same treatment,total fattiness content of leg muscle was higher than chest muscle about 1.53%,6.91%and 1.88%in the same treatment.(3) Using soybean oil as energy adding,the TG,LDL and HLDL in serum were showed no significantly differences (p＞0.05) between these treatments at 21d.At 70d,the AST,LDL TC AKP and HLDL in serum were showed no significantly differences(p＞0.05).(4) IGF—Ⅰlevel in serum at 21d was higher than that at 70d each treatment.IGF-Ⅰlevel showed no significantly differences(p＞0.05) at 21d,while it showed significantly differences(p＜0.05) at 70d,; female landes geese's serum GH level at 21d was increased with ME.
Experiment 3 Effect of Energy on Xupu Geese Growth Performance on 0-70 Days Stage
Objective:To research effect of different ME level on growth performance,the carcass trait,body measurement,serum Parameters,hormone level and muscle composition of Xupu geese in pre-overfeeding(0-70 days).Methods:According to the design of experiment 1 above.Results:(1) The best ME to Xupu geese is 13.38MJ/kg (0-21d) and 13.81MJ/kg(22-70d) in order to obtaining the best growth performance.(2) The carcass rate,eviscerated carcass rate,semi- eviscerated carcass rate,abdomen fat ratio and chest muscle rate were significant(p＜0.05) compared to the second group and the other two groups.As for subcutaneous fat layer thickness and intermuscle fat thickness,diversity were significant(p＜0.05) amone three groups.Abdomen fat weight and abdomen fat ratio of the second group were high than other two groups(p＜0.05).(3) Protein content of breast and leg muscle of Xupu geese which intake different energy diety has not significant diversity among three groups.Total lipids of chest muscle of the first group higher 4.46%,8.66%than the second and the third group,respectivelly.Total lipids of leg muscle of the first group higher 4.99%,11.63%than the second and the third group respectivelly.(4)There have no significant differences(p＞0.05) of IGF-Ⅰlevels at 21d or 70d.GH levels in serum of Xupu geese at 21d were low than its at 70d. Conclusions of Experiment 1-3:(1) The best ME to male geese is 12.13MJ/kg(0-21d) and 12.55MJ/kg(22-70d) in order to obtaining the best growth performance.The best ME to female geese is 13.38MJ/kg(0-21d) and 13.81MJ/kg(22-70d) in order to obtaining the best growth performance.The best ME to Xupu geese is 13.38MJ/kg(0-21d) and 13.81MJ/kg(22-70d) in order to obtaining the best growth performance.(2)In this experiment,ME could influence body sizes in limited range;(3) ME influence some parameters which have closely relation with growth performance like slaught weight of male landes geese,eviscerated carcass weight,semi-eviscerated carcass weight,breast muscle weight,leg muscle weight and abdominal fat weight of landes geese were showed significantly differences(p＜0.05) between these treatments;but dressed carcass percentage,eviscerated carcass weight rate,semi-eviscerated carcass weight rate,leg muscle rate of landes geese were showed no significantly differences(p＞0.05) which effected by ME level.(4)ME levels influenced Xupu geese's dressed carcass percentage, eviscerated carcass weight,semi-eviscerated carcass weight rate,abdominal fat rate and breast muscle rate significantly(p＜0.05).(5)Muscle lipid content of landes geese was higher than it of Xupu geese.CP,total lipid and DM content of male landes geese were higher than those of female landes geese,and the chemical constitution of geese muscles would changed with ME levels.(6) GH levels in serum were influenced by ME levels, while there was a positive relationship between them;IGF-Ⅰlevels in serum has no relationship with ME levels.
Experiment 4 The Research of MSTN Gene Clone and energy Influence on MSTN Gene Expression in Landes Geese and Xupu Geese
Objective:Cloning of MSTN gene of Landes geese and Xupu geese the relationship was researched between MSTN gene expression and different energy diets or IGF-Ⅰ,GH in serum in order to know the function of MSTN gene and provide information for molecular nutriology and breeding of waterfowl.Methods:Genome RNA was extracted from muscle of Landes geese and Xupu geese,MSTN gene mRNA was amplified by using RT-PCR,the PCR product was cloned into pGEM-T vector.The MSTN gene was isolated and sequenced from the positive clones screened Based on the MSTN gene clone, usingβ-actin as inner control,constructing an optimal semi-quantitative RT-PCR method, the difference of MSTN gene expression at 21 and 70 days by feeding different energy diets,at one time we detected IGF-Ⅰand GH in serum.Results:(1) The unique DNA fragment about 1128bp in length was obtained.Sequence analysis revealed that it has a length of 1128 nucleotides which encodes a 375 amino peptide.Compared with homologous sequence of human,rat,duck and goose,it displayed a fairly high degree conservation.The homologue of the nucleotide sequence are 94%,94%,99%respectively, the homologue of the amino acid sequence are 98%,97%,98%sespectively.(2) At 21 days,the expressions of MSTN in Landes geese or Xupu geese were showed no significantly differences.(3) At 70 days,the expression of MSTN in male wasⅠ＞Ⅲ＞Ⅱby 70d,to female wasⅢ＞Ⅱ＞Ⅰby 70d,to Xupu geese wasⅢ＞Ⅱ＞Ⅰby 70d.(4) In 21～70 days period,the expression of MSTN was changed with IGF-Ⅰin serum,it was not associated with GH in serum.GH was significant correlations with energy.Conclusions: The different energy influenced MSTN gene expression of landes geese and Xupu geese; in this period,the MSTN gene expression was associated with IGF-Ⅰin serum,but it was not associated with GH in serum.
引文
1.Ahreus EH Jr,Blankenhorn DH,Effect on human serum lipids of substituting plant for animal fat in diet,Proc Soc Wxp Biol Med 86:872-878.
2.Baeza E,Juin H,Rebours G,Constantin P,Marche G,Leterrier C.Effect of genotype,sex and rearing temperature on carcase and meat quality of Guinea Fowl.Brit Poult Sci,2001,42(4):470-476.
3.Abdullah Y,Abdullah,Hussein S,Musallam.Effect of different levels of energy on carcass composition and meat quality of male black goats kids.Livest Sci,2007,107:70-80.
4.Anthony Guernec,Bernadette Chevalier,Michel J Duclos.Nutrient supply enhances both IGF-I and MSTN mRNA levels in chicken skeletal muscle.Domestic Animal Endocrinology.2004Mar;26:143-54.
5.Baly DL,Horuk R,The biology and chemistry of the glucose transports.Biochim Biophys Acta,1998,947:571-590.
6.Barb C R.The Brain-Pituitary-Adipocyte Axis:Role of Leptin in modulating neuroendo-crine function.J Anim Sci,1999,77:1249-1257.
7.Berry,B W,Smith G.C,Cross H R.Constant time versus constant temperature cooking of beef loin steaks as influenced by marbling characteristics and intramuscle fat contant.J Anim Sci,1981,52(5):1034-1040.
8.Burks TF,Galligan JJ,Porreca F,Barber WD,Structure,biosynthesis,and glycosy-lation of human small intestinal mAS Tase-glucoamylase.J Biol Chem 1985(263):19709-19717.
9.Campion DR.Novakofki J.Technical perspective of biotechnology for control of growth and product quality in meat product.Biotechnology for Control of Growth and Product Quality in Meat Production:Implications and Acceptability.Pvan der Wal,GM Weber,F J van der Wilt.Pudoc Wageningen.1991.17-18.
10.Carlson,C,FW BOTH,SE Gordon,Skeletal muscle myostatin Mma Expression is fiber-type specific and increases during hindlimb unloading,American Journal of Physiology,1999277:R601-R606.
11.Chen E Y,Liao Y C,Smith D H,et al.The human growth hormone locus:nucleotide sequence,biology and evolution.Genomics,1989,4,479-497,
12.Clark SD,Jump DB,Regulation of gene transcriptionby polyunsaturated fatty acids.Prog Lipid Res 1993,32:139-149
13.Decaux J-F,Antoine B,Kahn A,Regulation of the expression of the L-type pyruvate kinase gene in adult rat hepamcytes in primary culture.J Bioi Chem 1989;246:11584-90
14. Dessay E, Wacrenier N, Marche G. Effect of Selection for Improved Body Weight and Composition on Muscle and Meat Characteristics in Muscovy Duck . British Poultry Science, 2002,43 (4) : 560-568.
15. Emilia KOLLAROVA, Zuzana SALAGOVA. Effect of energy intake on lipid profile in pigs. Special Number Proceedings of the International Scientific Conference on the Occasion of the 55th Anniversary of the Slovak Agricultural University in Nitra. 2001, Acta fytotechnica et zootechnica
16. Frahat A, Chavez E R. Effects of line , Dietary protein , sex, age and feed withdrawal on IGF-I in white Peking Ducks[J ] .Poult Sci, 1999, 78 :1307-1312.
17. Freitas, Almir Chalegre de, FUENTES, Maria de Fatima Freire, FREITAS, Ednardo Rodrigues, sucupira, Francislene Silveira, OLIVEIRA, Bruno Cesar Moura de, ESPINDOLA, Gastao, Barreto. Dietary crude protein and metabolizable energy levels for meat quails. Rev Bras Zootecn, 2006,35 (4) : 1705-1710
18. Fu Z W, Tadashi N , Hisanori K. Vitamin A deficiency reduces IGF-I gene expression and increases IGF-I receptor and insulin receptor gene expression in tissues of Japanese quail, J Nutr, 2001,131:1189-1194.
19. Gheisari, A. A., Saraeian, O., Toghiani, M. Response of male broiler breeder chicks to diets containing different levels of energy, protein and sulfur amino acids. J Sci Technol Agric Nat Res, 2005,9 (3) : 185-195
20. Gla-ert HP,Dielary tat,gene expression, and carcino-genesis. In Berdanier CD,Hargrove JL (eds), Nutrition and gene expression.CRC Press,Boca Raton,FL, 1993,247-268
21. Goda T,Bustament S,Koldovsky O.Dietary regulation of intestinal lactase and sucrase in adult rats:quantitative comparison of effect of lactose and sucrose,J Pediatr, Gastroenterol Nutr,1985,4:998-1008;
22. Grobet L,Martin L J,Poncelet D.A deletion in bovine myostatin gene causes the double-muscled phenotype in cattle. Nature Genetics, 1997,17:71:74.
23. Grundy SM,Vega GL,Two different views of the relationship of hypertriglyceridemia to coronary heart disease,implication for treatment,Arch intern Med, 1992,152:28-34
24. Havel RJ,Kane IP,Structure and metabolism of plasma lipoproteins,In Scriver CR,Beaudet AL,Sly WS,Valle D ( eds) ,The meatbolic basis of inherited disease,7th ed,McGraw-Hill,New York,1995,1841-1851
25. Havenstein G B, Ferket P R, Qureshi M A. Growth, livability and feed conversion of 1957 Versus 2001 broilers when fed "typical" 1957 and 2001 broiler diets. Poult Sci, 2003, 82 (10) : 1500-1508
26. Hossain M E, Shahjalal M, Khan M J, Bhuiyan A A. Effect of dietary energy supplementation on feed intake, growth and reproductive performance of sheep under grazing condition. Pakistan J Nutr,2003,2 (3) : 148-152
27. Hu L,Guo DX,Hu R,Liu M,Wang L.Luan XH,Expression rule of MSTN gene in big bones chicken,animal technology, 2003,20 (11), 42-44 (in Chinese). Lauterio TJ ,Scanes CG Journal Nutrition, 1987,117:758 -763.
28. Izevbigi E B, Robbbins K R. Effect of season on metabolizable energy requirement for broiler growth, carcass yield and carcass composition. Tennessee Farm Home Sci, 1988,148:8-12
29. Jenn-Chung Hsu, Ta-Wei LU, Peter W S, Chiou,et al. Effect of different sources of dietary fibre on growth performance and apparent digestibility in geese.Animal Feed Science Technology, 1996,60:93-102
30. Jensen L S, Wyatt C L, Fancher B I. Sulfur amino acid requirement of broiler chicks from 3 to 6 weeks of age. Poult Sci, 1989, 68:163-168
31. Jeroch H, Schubert R, Prinz M, Petzod M. Energy and crude protein requirements of young fattening geese. 2. Influence of varying energy and crude protein contents of feed on carcass quality. Arch Tieremahr, 1978,28 (2) : 111-122
32. Ji S.Myostatin expression in porcine tissues:tissue specificity and developmental and postnatal regulation American Journal of Physiology, 1998,275: R1265-R1273.
33. Jiang S Q, Ding F Y, Lin Y C. Study on requirement of metabolizable energy for 0-21-day-old Lingnan yellow broiler chicks. J South China Agric Univ, 2003,24 (2) : 73-76
34. John P,McMurty. Nutritional and developmental roles of insulin-like growth factors in poultry.Journal of Nutrition, 1998,128 :302S - 305S.
35. Kambadur R,Sharma M,Smith T P.Mutations in myostatin in double-muscled Belgian Blue and Pledmontese cattle .Genome Research, 1997,7 (9) :910-916.
36. Katoh K, Obura YC. Developmental and nutritional astral of somatotropic axis in the ruminant [ J ]. Asian,Aust J Anim Sci, 2001,14 (Special issue ) : 91 -99.
37. Kauffman R G, Carpenter Z L, Bray R W, Hoekstra W G Biochemical properties of pork and their relationship to quality intramuscular fat. J Food Sci, 1963,29: 70-74
38. Kim W K, Kim M H, Seo D S, Lee C Y, Suk Y O, Ko Y. Associations between feed efficiency, body growth and serum insulin-like growth factor-I level for Korean native ogol chickens. Asian Austral JAnim Sci, 2005, 18 (4) : 532-517
39. Kinsell LW,GD MIchael,Letter to editor,Am J Clin Nutr, 1955, 3:247-253
40. Kita K, Ivoda C, Miki K, et al. Relationship of IGF-I mRNA levels to tissue development in chicken embryo of different strains .Asian-Aust J Anim Sci. 2000,13 :1653-1658 .
41. Kita K, Okumuru J. Chicken insulin-like growth factor-I stimulates protein synthesis of chicken embryo myoblasts culture in serum-free medium .Asian-Aust JAnim Sci. 2001,14 :17-20 .
42. Kiyoshi K, Frances B C, Yoshitaka K J, et al. Expression of insulin-like growth factor-I during chicken development .Endocrinology. 2001,142 :1234-1239 .
43. Kocamis H, Kirkpatrick D C, Klandorf H, et al. In Ovo Administration of recombinant human IGF-I alters postnatal growth and development of the broiler chicken .Poult Sci. 1998, 77:1913-1919.
44. Larzul C,Rouvier R,Guy GEstimation of genetic parametersfor growth carcass and over feeding traits in a white goosestrain.Genetics Selection Evolution 2000,32 (4) :415-427.
45. Latorre M A, Lazaro R, Valencia D G, Medel P, Mateos G G The effects of gender and slaughter weight on the growth performance, carcass traits, and meat quality characteristics of heavy pigs. J Anim Sci, 2004, 82 (2) : 526-533
46. Lauterio TJ ,Scanes CG Hormonal responses to protein restriction in two strains of chickens with different growth. characteristics.Journal Nutrition, 1987,117 :758-763.
47. Lee C Y, Lee H P, Jeong J H, et al. Effects of restricted feeding, low-energy diet and implantation of trenbolone acetate plus estriol on growth, carcass traits, and circulating concentrations of insulin-like growth factor ( IGF )-I and (IGF)-binding protein-3 in finishing barrows .J Anim Sci. 2002, 80 :84-93 .
48. Li Q F, Xie Z, Qiang Ba Y Z, et al. Study on the Correlation between Blood Enzyme Activity and Production Performance in Tibetan Yaks. Developmental & Reproductive Biology, 2003,11 (1): 8-12.
49. Life Sciences Research Office.The evaluation of the energy of certain sugar alcohols used as food ingredients.Federation of American Societies for Experimmental Biology,Bethesda,MD. 1994
50. Lilmatta M,Towle HC,Clarke S,Jump DB.Dietary polyunsaturated fatty acids interfere with the insulin/glucose activation of L-type pyruvate kinase gene transcription.Mol Endocrinol 1994; 8:1147-53
51. LIN J,ARNOLD H B,DELLA-FERA M A. Myostatin knockout in mice increases myogenesis and decreases adipogenesis. Biochemical and Biophysical Research Communica- tions.2002,291 (3) .701-706.
52. Liu Z,Thompson KS,Towle HC,Carbohydrate regulation of the rat L-type private kinase gene requires two nuclear factors:LF-Al and a member of the c-myc family.J Biol Chem 1993; 268:12787-95
53. Lu WF.Porcine Myostatin gene Expression and its Location in Different Tissues.Ph D dissertation,Veterinary College of PLA. 2003,11-13.
54. Ma XY, Cao YC, Shu DM,Bi YZ, Clonging and expression of myostatin gene and animal immunity experiment,Science in china series c, 2004,34 (6) :522-526. (in Chinese)
55. Ma XY, Shi ZD, Cao YC, Bi YZ, Ma JY, Cloning and sequencing analysis of myostatin gene of chicken and goose, journal of south china agricultural university (natural science edition) , 2004, 4 (2) ,85-88. (in Chinese)
56. Mazumder N K, Mazumder A. Relational between Plasma Alkalinephospha Tase White Enzyme Activity in White Leghorn Chicken. Indian J Ani Sci, 1991,61 (3) : 299-300.
57. Mcguinness M C, Lreg D W, Balon W L. Effects of factor and refeeding on the somatotropic and thyroidares in Juvenile Chickens . Poult Sci, 1998 , 77 (suppl 1) : 49.
58. McMurtyJP. .J Nutr, 1998 ,128 :302S-305S;
59. Mcpherron A C, Lawler A M, Lee S J. Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member. Nature, 1997,3 87 (6628) :83-89.
60. McPherron A C, Lee S J. Double muscling in cattle due to mutations in the myostatin gene. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94: 12457-12461.
61. Mcpherron A C,LEE S J. Suppression of body fat accumulationin myostatin-deficient mice. The Journal of Clinical Investigation,2002,109 (5) .595-601.
62. Mendes A A, Moreira J, de Oliveira E G. Effect of dietary energy on performance, carcass yield and abdominal fat of broiler chickens. Rev Bras Zootecn, 2004,33 (6) : 2300-2307
63. Mensink RP,Katan MB.Effects of dietary fatty acids on serum lipids and lipoproteins:a meta-analysis of 27 trials.Arteriosclerosis,1992,12:911-919
64. Mignon-Grasteau S, Beaumont C, Le Bihan-Duval E, Poivey JP, De Rochambeau H, Ricard FH.. Genetic parameters of growth curve parameters in male and female chickens. Brit Poult Sci , 1999,40 (1) : 44-51
65. Mohanna C, Nys Y. Effect of dietary zinc content and sources on the growth, body zinc deposition and retention, zinc excretion and immune response in chickens . British Poultry Science, 1999,40 (1) : 108-114.
66. Morrill JS,Kwong LK,Sunshine P,et al. Dietary CHO and stimulation of carbohy- drates along cillus column of fasted rat jejumum,AM J Physiol,1989,256:G158-G165;
67. Nahashon S N, Adefope N, Amenyenu A N, Wright D. Effects of Dietary Metabolizable Energy and Crude Protein Concentrations on Growth Performance and Carcass Characteristics of French Guinea Broilers. Poult Sci, 2005,84 (2) : 337-344
68. Nahashon S N, Adefope N, Amenyenu A, Wright D. Effect of varying metabolizable energy and crude protein concentrations in diets of Pearl Gray guinea fowl pullets 1. Growth performance. Poult Sci, 2006c, 85 (10) : 1847-54
69. Nahashon S N, Aggreg S E, Adefope N A, Amenyenu A. Modeling Growth Characteristics of Meat-Type Guinea Fowl. Poult Sci, 2006b, 85 (5) : 943-946
70. Nahashon S N, Aggrey S E, Adefope N A, Amenyenu A, Wright D. Growth Characteristics of Pearl Gray Guinea Fowl as Predicted by the Richards,Gompertz,and Logistic Models.Poult Sci,2006a,85(2):359-363
71.Nahashon S,Adefope N,Amenyenu A,Lema M,Wright D.Growth and carcass characteristics of French guinea broilers fed diets with varying concentrations of metabolizable energy.J Sustain Agile,2006d,27(4):25-43
72.Nawaz H,Mushtaq T,Yaqoob M.Effect of Varying Levels of Energy and Protein on Live Performance and Carcass Characteristics of Broiler Chicks.Poult Sci,2006,43(4):388-393
73.Nguyen T V,Bunchasak C.Effects of dietary protein and energy on growth performance and carcass characteristics of Betong chicken at early growth stage.Songklanakarin J Sci Technol,2005,27(6):1171-1178
74.NRC编:《家禽营养需要》(第九次修订版),蔡辉益等译,中国农业科技出版社,1994
75.Nutrient Requirements of Poultry,Ninth Revised Edition,1994,National Research Council,1994.
76.Panda A K,Niranjan M,Reddy B L N,Sharma R P.Influence of dietary energy on growth,immunecompetence and carcass characteristics of coloured broiler chickens.Anita Nutr Feed Technol,2006,6(1):115-121
77.Persia M E,Dehority B A,Lilburn M S.The Effects of Enzyme Supplementation of Corn-and Wheat-Based Diets on Nutrient Digestion and Cecal Microbial Populations in Turkeys.Journal of Applied Poultry Research,2002,11(2):134-145.
78.Rausch M I,Tripp M W,Goroni K E.The influence of level of feeding on growth and serum insulin-like growth factor-Ⅰ and insulin-like growth factor binding proteins in growing beef cattle supplemented with somatotropin.J Anim Sci.2002,80:94-100.
79.Ren J B,Zhao G Y,Li Y X,Meng Q X.Influence of dietary lysine level on whole-body protein turnover,plasma IGF-I,GH and insulin concentration in growing pigs.Livest Sci,(2006),doi:10.1016/j.livsci.2006.10.009
80.Rios R,Cameiro I.Myostatin is an inhibitor of myogenic differentiation.American Journal of Physiology Cell Physiology,2002,282(5):C993-999.
81.Roberson R H,Francis D W.The effect of protein level,iodinated casein and supplemental methionine on the performance of White Chinese geese.Poult Sci,1963,42:863
82.Saleh E A,Watkins S E,Waldroup A L,Waldroup P W.Effects of Dietary Nutrient Density on Performance and Carcass Quality of Male Broilers Grown for Further Processing.Int J Poult Sci,2004,3(1):1-10
83.Schubert R,Jeroch H,Hennig A.Studies of the energy and crude protein requirements of young fattening geese.Arch Tierernahr,1977,27(12):720-730
84.Scott M L,Nesheim M C,Young R J.Nutrition of the chicken.3rd ed.New York:Scott and Associates,1982
85. Se-Jin Lee.Alexandra C Mcpherron.Myostatin and the control of skeletal muscle mass.Current Opinion in Genetics & Development, 1999,9:604-607.
86. Semanza GS,Auricchio S.Small intestinal disaccharidases.In Scriver CR, Beauset AL,Sly WS,Valle D (eds) ,The metabolic basis of inherites disease,6th ed. McGraw Hill,New York,1989,2975-2997;
87. Shaoquan, J, RL Losinski, S G Comelius, et al.Myostatin expression in porcine tissues: tissue specificity and developmental and postnatal regulation. American Journal of Physiology 1998,275:R1265.
88. Sharma M,Kambadur R,Matthews K GMyostatin,a transforming growth factor-β superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct. Journal of Cellular Physiology, 1999,180:1 -9.
89. Sonstegard TS,Rohrer GA, Smith TP. Myostatin maps to porcine chromosome 15 by linkage and physical analyses. Anim Genet, 1998,29 (1) : 19-22.
90. Stevenson M.H. Effects of diets of varying energy concentrations on the growth and carcass composition of geese. Brit Poult Sci, 1985,26:493-504
91. Stick C D, Martin J L Baxter R C. Production of IGF-I and its binding protein by breeding cow hepatocytes in primary culture. Endocrinology, 1998,16 (3) : 1094-1101
92. Tall AR.Plasma cholesterol ester transfer protein.J Lipid Res, 1993,34:1255-1274
93. Tesseraud S , Pym R A E, Bihan E L, et al. Response of broilers selected on carcass quality to dietary protein supply :Live performance , muscle , development, and circulating in sulin like growth factors (IGF-Ⅰ and Ⅱ) Poult Sci,2003,82:1011-1016.
94. ThomasM,L angley B,Berry C.Myostatin, a negative regulator of muscle growth, functions by inh ibiting myoblast proliferation. Journal of Biological Chemistry, 2000; 275:40235-40243.
95. Vasilators-Younken R, Scanes C G Growth hormone and insulin-like growth factors in poultry growth: required, optimal, or ineffective. Poult Sci, 1991,70 (8) : 1764-1780
96. Vasilatos2younken R. Absence of growth hormone2induced avian muscle growth in vivo [J ] . Poult Sci, 1999, 78 : 759-768.
97. Vasilatos-younken R. Absence of growth hormone-induced avian muscle growth in vivo .Poult Sci. 1999, 78 :759-768 .
98. Vaulont S,Puzenat N,Levrat F,et al.Proteins binding to the liver-specific pyruvate kinase gene promoter:a unique combination of known factors.J Mol Biol 1989; 209:205-19.
99. Waldroup P W, Tidwell N M, Izat A L. The effect of energy and amino acid levels on performance and carcass quality of male and female broilers grown separately. Poult Sci, 1990,69:1513-1521
100. Wang W J, Huang L S, Chen K F, et al. Polymorphism of insulin-like growth factor-I Gene in 13 pig breeds and its relationship with pig growth and carcass traits .Asian-Aust J Anim Sci. 2002, 15:1391-1394.
101.Whang K Y,Donovan S M,Easter R A.Effects of protein deprivation on subsequent efficiency of dietary protein utilization in finishing pigs.Asian-Aust J Anim Sci.2000,13:659-665.
102.Yab Lanski T.Correlation between the Activities of the Plasma Enzymes GOT,GPT,AKP and Some Performance Qualities in Pigs.Col Sci Works,1986,(30):599-616.
103.Yalcin S,Ozkan S,Acikgoz,Z,Ozkan,K.Influence of dietary energy on bird performance,carcass parts yields and nutrient composition of breast meat of natural optimum and summer temperature.Brit Poult Sci,1998,39(5):633-638
104.Yamada K,Goda T,Bustamente S,Koldovsky O(1983).Different effectof starvation on activity of sucrase and lactase in rat jujunoileum.AM J Physiol 245:G449-G455;
105.Yu B.Effect of Different Sources of Dietary Fiber on Growth Performance,Intestinal Morphology and Caecal Carbohydeases of Domestic Geese.British Poultry Science,1998,39(4):560-567.
106.Zanusso J T,Oliveiva R F M,Donzele J L.Metabolizable energy levels for broilers(1 to 21days) maintained under a thermoneutral enviroment.Rev Bras Zootecn,1999,28(5):1068-1074
107.Zhang KY,Cheng DW.Insulin-like growth factor and its nutritional manipulation,Chinese Journal of Animal Science,2002,38(6),42-44.(in Chinese)
108.Zimrners T A,M V Davies,L G Koniaris,P Haynes,A F ESquela,K N Tomkinson,A C Mcpherron,N M Wolfman,Se-Jin LEE,(2002).Induction of Cachexia in mice by systemically administered myostatin.Science,296(5572):1468-94.
109.曹爱青,毛宗林,胡骏鹏,范卫星,何瑞国,不同能量水平对朗德鹅生长曲线的影响研究,饲料工业,2007第28卷第7期,34-37.
110.陈国宏,卞建春,王克华等.地方鸡种血浆AKP和GPT及CPK活力比较研究.西北农业大学学报,1998,26(4):66-70.
111.陈国宏.中国部分地方鸡种肉品质性状比较研究.[博士学位论文].杨凌:西北农业大学,1997
112.陈继兰,吕连山,赵玲,方丽.石歧黄肉鸡前期日粮适宜的能量和蛋白质水平的研究.中国畜牧杂志,1998,34(4):10-12
113.陈金文,杨山,莫棣华,文伯珍,赵河山,胡刚安.日粮能量和蛋白水平对肉鸡腹脂和血脂的影响.动物营养学报,1998,10(1):20-28
114.陈耀王.高档营养食品鹅肥肝的生产.科学技术文献出版社,北京.1987.
115.程红娜.合浦鹅饲粮能量、粗蛋白质、钙和磷适宜水平的研究.[硕士学位论文].南宁:广西大学,2005.
116.邓兴照,彭华,刘福柱.类胰岛素生长因子-Ⅰ及其营养调控,中国畜牧杂志,2005,41(3),39-41.
117.杜卫国,鲍毅新,施利强,俞华英.社鼠内脏器官重量和水分含量的季节性变化.动物学杂志.1999,34(1):23-25.
118.范卫星,何瑞国,胡骏鹏,曹爱青,朗德鹅血液性状与体脂沉积间相关关系的研究,饲料工业,2007第28卷第15期,36-38.
119.范卫星,何瑞国,胡骏鹏,曹爱青等,日粮能量水平对超饲养前溆浦鹅脂肪代谢的影响研究,中国饲料,2007第13期,18-21.
120.方立超.不同的饲粮能量和蛋白质水平对肉鸡肉质及痛风症发生率的影响.[硕士学位论文].重庆:西南农业大学,2001.
121.房兴堂,王景明,雷丛,张海琪,刘玉梅,孙刚,王立强.肉鸡消化器官生长规律的研究.畜禽业,1999(1):12-12.
122.房兴堂,王景明,雷丛,张海琪,刘玉梅.肉鸡内脏器官生长规律的初步探讨.中国家禽,1998,20(6):10-12.
123.付予华,刘琦山,荣雷,李吕木,吴剑平,张明生.不同能蛋水平日粮对皖西白鹅早期增重的试验.中国家禽,1991(5):29-31.
124.高波.新太湖鹅不同世代肉用性能改进效果及RAPD和活性研究.[硕士学位论文].扬州:扬州大学,2000.
125.葛剑,谷子林,李英,魏忠华,郑常山.河北柴鸡1-16周龄生长曲线分析与拟合的比较研究.中国家禽,2005,27(14):9-11.
126.呙于明.家禽营养[M].北京:中国农业大学出版社.1997,19-20.
127.郝正里,肖金玉,项光华等.维生素A、D、E添加水平对产蛋鸡血清与蛋黄生化指标的影响.甘肃农业大学学报,2000,35(3):243-250.
128.何瑞国,刘祥友,李翔,赵阿勇,赵娜,张巍,周樱.坚持科学发展观,持续发展湖北水禽业.中国农学通报,2005,21(增刊):210-213.
129.候曼玲.食品分析.化学工业出版社.北京.2004.5.
130.胡骏鹏,何瑞国,范卫星,曹爱青等,豆油能量对朗德鹅超饲养前期生长性能,血清参数及其相关激素水平的影响研究,中国粮油学报(待发).
131.胡骏鹏,何瑞国,范卫星,曹爱青等溆浦鹅肌肉生成抑制因子(MSTN)基因的克隆及其表达量与日粮能量,血清IGF-I和GH的关系研究,畜牧兽医学报(待发).
132.胡骏鹏,李翔,范卫星,曹爱青,赵阿勇,何瑞国等,朗德鹅肌肉生成抑制因子(MSTN)基因的克隆及其表达量与日粮能量,血清IGF-I和GH的关系研究,中国农业科学(待发).
133.胡兰,郭东新,胡锐,刘梅,王娜,栾新红.大骨鸡中MSTN基因表达规律性的研究.动物科技,2003年第20卷第11期,42-44.
134.黄建亮.江山白鹅早期生长发育特性的研究.中国草食动物,2000,2(4):20-21.
135.蒋守群,丁发源,林映才,余德谦,杨晓建.能量水平对0-21日龄岭南黄鸡生产性能、胴体品质和体组成的影响.中国家禽学报,2003,7(1):83-86.
136.井文倩.三类型黄羽肉鸡适宜能量水平的比较研究.[硕士学位论文].泰安:山东农业大学,2002
137.孔保华等,肉制品工艺学.黑龙江科学技术出版社,哈尔滨.1996.
138.李福昌,王悦忠,颜炳军.AA肉仔鸡肌肉酸度的测定报告.中国家禽,1993,(5):24-25.
139.李俊波,左绍群,张克英.生长前期丝羽乌骨鸡饲料适宜能量、蛋白水平研究.动物营养学报,2000,12(3):37-43.
140.李文立,王宝维,刘光磊,等.不同钙磷水平对0-4周龄五龙鹅生产性能及血浆和胫骨生化指标的影响.动物营养学报,2004,17.
141.李文立,王宝维,刘光磊,等.不同钙磷水平对5-8周龄五龙鹅生产性能及血浆和胫骨生化指标的影响.中国农学通报,2005,21(3):15-19.
142.李翔,糙米型饲粮对鹅鸭肥肝性能影响及朗德鹅肥肝脂肪沉积规律的研究,华中农业大学2005届博士论文,12-16.
143.廖玉英,杨家晃,何仁春,秦黎梅,麦伟虹,卢桂猷.日粮代谢能水平对鹅生长性能、养分利用率及肉质的影响.粮食与饲料工业,2006(5):38-41.
144.林树茂等,不同禽肉脂肪酸组成的比较研究.中国畜牧杂志,2004,40(12):18-20.
145.刘定发,柳小春,施启顺,陈斌,王治平.番鸭血浆酶活性及其与产肉性能关系的研究.当代畜牧,1999,(2):41-42.
146.刘建文,施用晖,乐国伟.动物生长轴的激素调控.中国饲料,2003,(14):7-9.
147.刘思扬.肉用狮头鹅技术产量指标测试初报.广东畜牧兽医杂志,2000,9(25):3.
148.刘翔友,何瑞国等,日粮粗纤维对朗德鹅生产性能和血清参数影响的研究,中国粮油学报,2006,6,21(3),178-182.
149.刘雨龙,刘惠兰.蛋鸡育成阶段内脏器官的生长分析.饲料与畜牧,1995,(1):9-12.
150.刘玉兰,冯定远.性别对生长肥育猪生长性能、胴体品质和肌肉营养成分的影响.粮食与饲料工业,2005,(3):39-41.
151.卢德勋.系统动物营养学导论.北京:中国农业出版社出版,2004.
152.吕明斌,黄启贤.日粮添加大豆油对肉用仔鸡的饲喂效果.中国饲料,1997,(12):17-18.
153.吕文发.猪肌生成抑制素(Myostatin,MSTN)表达及组织定位研究,博士学位论文,中国人民解放军军需大学,2003,11-13.
154.罗庆斌,何大乾,尹荣楷,龚绍明,孙国荣.我国养鹅业现状及发展趋势.中国家禽,2006,28(4):1-4.
155.麻艳群.饲粮营养水平、纤维来源对鹅利用纤维潜力和生产性能的影响.[硕士学位论文].南宁:广西大学,2005.
156.马国龙,孙鹏翔.肉用中型仔鹅能量和蛋白质需要量的研究.水禽世界,2006,(2):33-37.
157.马现永,曹永长,舒鼎铭,毕英佐,肌肉生成抑制因子基因的克隆、表达及动物免疫实验,中国科学,C辑,2004,34(6):522-526.
158.马现永,施振旦,曹永长,毕英佐,马静云.鸡、鹅肌肉生成抑制因子基因的克隆和序列分析,华南农业大学学报(自然科学版)2004年4月第25卷第2期,85-88.
159.毛国祥,陈柯,赵万里.隆昌鹅、太湖鹅及新太湖鹅部分血液生化指标研究.动物科学与动物医 学2000,17,(5):18-20.
160.毛国祥,赵万里.太湖鹅、隆昌鹅及新太湖鹅生长规律研究.动物科学与动物医学,2001,18(2):11-13.
161.毛国祥,赵万里.新太湖鹅,太湖鹅和隆昌鹅肌肉品质比较研究.动物科学和动物医学,2000,17(1):16-19.
162.米玉玲,王安.低温高能饲粮对笼养产蛋鸭生产性能及生化指标的影响.动物营养学报,2003,15(1):21-25.
163.闵育娜,侯水生,高玉鹏,黄苇,赵玲,喻俊英.0-4周龄肉仔鹅能量和蛋白质需要的研究.黑龙江畜牧兽医,2005a(6):26-27.
164.闵育娜,侯水生,高玉鹏,黄苇,赵玲,喻俊英.日粮能量蛋白水平对肉仔鹅胴体性能和血液生化指标的影响.西北农林科技大学学报(自然科学版),2005b,33(6):40-44.
165.莫棣华.对“广黄”鸡的能量和蛋白质需要的研究.广东农业科学学,1983,(3):27-30.
166.欧茶海等.不同日龄和性别腾冲雪鸡肉的营养成分分析.西南民族学院学报,1999,25(2):181-186.
167.欧阳克蕙,王文君,瞿明仁,范玉庆.油脂对泰和乌骨鸡生长后期生产性能的影响.江西饲料,2002(6):9-10.
168.祁永红.扬州鹅早期生长发育规律及其肉用性能的研究.[硕士学位论文].扬州:扬州大学,2001.
169.卿晓红等,三穗鸭肉、蛋及羽毛粉营养成分含量分析.贵州畜牧兽医,1996,20(5):11-12.
170.邱祥聘,周世永.丝羽乌骨鸡肉品质的研究概况.畜禽业,2002(7):8-9.
171.佘德勇.北京鸭和樱桃谷鸭生长性能、肌肉理化特性比较及填饲对其影响.[硕士学位论文].北京:中国农业大学,2005.
172.沈元新.绍鸭发育与营养研究.成都:成都科技大学出版社,1997:57-58.
173.盛志廉,吴常信.数量遗传学.北京:中国农业出版社,1995:104.
174.盛志廉,吴常信.数量遗传学.北京:中国农业出版社,1995:114-115.
175.宋成义,经荣斌.畜禽GH基因多态性与生产性能相关研究进展,国外畜牧科技,2000,27(2),25-27.
176.苏秀侠,张源久,于秀芳,生群.肉仔鹅日粮能量、蛋白和纤维水平的探讨.四川畜禽,1997(7):41-44.
177.孙逊,朱尚权.生长激素的结构与功能,国外医学-生理,病理科学与临床分册,1999,19(1):6-9.
178.孙永峰,陈少康,吴伟.肉用品系鹅生长发育与性状间相关分析.吉林畜牧兽医,2003,8:1-3.
179.王磊,王康宁.影响胃肠道发育因素的研究进展.饲料工业,2006,27(5):13-16.
180.王宝维,刘光磊,张名爱,等.五龙鹅快长系早期生长规律与酶活性关系.西南农业大学学报(自然科学版),2005,27(1):102-106.
181.王宝维,潘庆杰,朱新产,等.“五龙鹅”品种选育与研究进展.中国家禽,2002,24(18):28-32.
182.王宝维,王雷,贾晓晖等.五龙鹅早期生长发育与血清酶活性的关系,甘肃农业大学学报,2005,6 (3):297-301.
183.王秉栋.动物性食品卫生理化检验.中国农业出版社,北京,1994.
184.王林,陈光伟,吴信生,杨凤萍,王金玉,林大光,孔佩兰.肉兔血浆AKP、GPT活性与生产性能关系的研究.畜禽业,2003,(8):12-11.
185.王绮,张晓娜.胰岛素样生长因子的分泌及功能对人体的影响.临床荟萃,2006,21(12):900-901.
186.王淑萍,杨连玉,吴伟.日粮营养水平对雏鹅饲喂效果观察.吉林畜牧兽医,2002(5):1-3.
187.王彦文,宋志琪.日粮能蛋水平对肉仔鸡生产性能的影响.甘肃畜牧兽医,1996,26(1):1-4.
188.王阳铭,王琳,杨文清,宋金昌,李绶章.肉用仔鹅集约化饲养条件下的能量和蛋白质需要.西南农业学报,1999,12(2):103-111.
189.王元林,袁锦和,杨震,等.江苏太湖鹅产蛋期若干生理生化指标的测定(初报).南京农学院报,1983,(3):97-99.
190.王志耕等.皖西白鹅肉脂特性研究.畜牧兽医学报,2002,33(4):332-335.
191.王志跃,陈伟亮,白群安,王健,王欢莉.新扬州鸡生长模型的比较研究.黑龙江畜牧兽医,2002,(12):4-6.
192.闻芝梅,陈君石主译,《现代营养学》(第七版),人民卫生出版社,1998年4月第一版,44-46:49-58.
193.乌尼尔夫,芒来.动物生长激素的研究现状.畜牧与饲料科学,2005,5:37-39.
194.吴东,夏伦志,周明,等.中草药饲料添加剂对种鹅繁殖性能和血清生化指标的影响.粮食与饲料工业,2005,6:41-43.
195.吴信生,杨凤萍,王金玉,林大光,孔佩兰,张振华,沈幼章,翟频,娄志荣,朱满兴,朱瑾佳,潘雨来.肉兔血浆碱性磷酸酶活性比较及其与生产性能的研究.中国养兔杂志,2000,(6):14-16.
196.夏中生,麻艳群,杨家晃等.饲粮纤维来源对合浦鹅生产性能、血清生化指标和养分代谢的影响.畜牧与兽医,2006,38(7):7-10.
197.谢广富.鹅肉营养成分分析及营养价值评定,中国家禽,1999(4).
198.谢庄,杨茂成.不同杂交组合鹅生长规律的比较.南京农业大学学报,1994,17(1):83-87.
199.徐明生,欧阳克蕙,上官新晨,王文君,陈锦屏.油脂对泰和乌鸡生产性能和肌肉脂肪酸组成的影响.福建农林大学学报(自然科学版),2006,35(1):67-72.
200.许盛海,包文斌,程金花,黄军,张红霞,陈国宏.鸭生长激素(GH)基因编码区及调控区多态性分析,畜牧兽医学报,2007,38(9):907-912.
201.许盛海,陈国宏.鸡生长激素基因的研究进展,上海畜牧兽医通讯,2006,3:18-20.
202.颜炳学,邓学梅,费箐,胡晓湘,吴常信,李宁.鸡生长激素基因单核苷酸多态与生长及屠体性状的相关性,科学通报,2003,6,48(2):1304-1307.
203.杨凤.动物营养学(第二版).北京:中国农业出版社,2002.
204.杨光荣,李翠蓉.鹅血浆某些生化指标的测定.畜牧与兽医学报,2004,36(10):29.
205.杨加豹,张亚平,李定发,邓发强,王利琴.日粮能量水平对肉鸭生产性能影响及“标准曲线法”测 定饲用酶制剂的表观代谢能值.中国饲料,2004,(19):12-12.
206.杨凌,葛建东.不同能量水平对四季鹅育肥效果的影响.畜牧与兽医,1998,30(2):67.
207.杨宁.家禽生产学.北京:中国农业出版社.2002,289-292.
208.杨山,家禽生产学,中国农业出版社,1995.
209.叶绍辉,张华英,段纲,欧茶海,李润泉,丁自柏.60-90日龄不同性别腾冲雪鸡血液生化指标差异研究.云南农业大学学报,1999,14(4):345-348.
210.余斌.赖氨酸铜对仔猪生长的影响及其作用机理研究.广州:华南农业大学学位论文,2002.
211.俞照正,陈维虎,王亚琴,等.8周龄浙东白鹅体重体尺和免疫器官的发育研究.中国草食动物,2005,25(2):23-24.
212.张碧丽.生长激素与肾脏,国外医学-内分泌分册,1997,17(2):94-96.
213.张春雷,刘福柱,侯水生.育雏期不同能量蛋白质水平对肉鹅生产性能影响.中国饲料,2004,(18):24-25.
214.张红,龚道清,张军,万建洪,狄志钢,郑建高,谭国才.溧阳鸡生长曲线分析与拟合的研究.畜牧与兽医,2006,38(2):7-9.
215.张克英,陈代文.类胰岛素生长因子及其营养调控,中国畜牧杂志,2002年第38期第6卷,42-44.
216.张喜武.中国水禽业发展现状及对策.中国家禽,2005,27(10):1-3.
217.张显花.中草药饲料添加剂对仔猪生长性能和日粮养分消化率的影响.[硕士学位论文].武汉:华中农业大学图书馆,2005.
218.赵阿勇.朗德鹅对能量蛋白质和钙营养需要的研究.[硕士学位论文].武汉:华中农业大学图书馆,2004.
219.赵向红.有效温度与日粮能量及蛋白水平对肉鸡消化功能的影响.[硕士学位论文].北京:中国农业大学,2005.
220.中国预防医学科学院,营养与卫生研究所,食品成分表.1991.
221.周光宏.肉品学.北京:中国农业科技出版社.1998.
222.周桂莲,林映才,蒋守群,丁发源,郭吉余,余德谦,杨晓健.饲粮代谢能水平对22-42日龄黄羽肉鸡生长性能、胴体品质以及部分血液生化指标影响的研究.饲料工业,2004,25(3):35-38.
223.周洪松,赵益贤.根据雏鸡血清酶活性对蛋鸡进行早期选择的研究[J].畜牧兽医学报,1991,22(3):220-224.
224.毕英左,傅伟龙.动物科学进展.北京:中国农业科技出版社,2000.
225.朱志明,强巴央宗,朱猛进,纪素玲,唐晓惠,莫德林,刘榜.藏鸡生长曲线拟合和分析的比较研究.中国农业科学,2006,39(10):2159-2162.
2.Baeza E,Juin H,Rebours G,Constantin P,Marche G,Leterrier C.Effect of genotype,sex and rearing temperature on carcase and meat quality of Guinea Fowl.Brit Poult Sci,2001,42(4):470-476.
3.Abdullah Y,Abdullah,Hussein S,Musallam.Effect of different levels of energy on carcass composition and meat quality of male black goats kids.Livest Sci,2007,107:70-80.
4.Anthony Guernec,Bernadette Chevalier,Michel J Duclos.Nutrient supply enhances both IGF-I and MSTN mRNA levels in chicken skeletal muscle.Domestic Animal Endocrinology.2004Mar;26:143-54.
5.Baly DL,Horuk R,The biology and chemistry of the glucose transports.Biochim Biophys Acta,1998,947:571-590.
6.Barb C R.The Brain-Pituitary-Adipocyte Axis:Role of Leptin in modulating neuroendo-crine function.J Anim Sci,1999,77:1249-1257.
7.Berry,B W,Smith G.C,Cross H R.Constant time versus constant temperature cooking of beef loin steaks as influenced by marbling characteristics and intramuscle fat contant.J Anim Sci,1981,52(5):1034-1040.
8.Burks TF,Galligan JJ,Porreca F,Barber WD,Structure,biosynthesis,and glycosy-lation of human small intestinal mAS Tase-glucoamylase.J Biol Chem 1985(263):19709-19717.
9.Campion DR.Novakofki J.Technical perspective of biotechnology for control of growth and product quality in meat product.Biotechnology for Control of Growth and Product Quality in Meat Production:Implications and Acceptability.Pvan der Wal,GM Weber,F J van der Wilt.Pudoc Wageningen.1991.17-18.
10.Carlson,C,FW BOTH,SE Gordon,Skeletal muscle myostatin Mma Expression is fiber-type specific and increases during hindlimb unloading,American Journal of Physiology,1999277:R601-R606.
11.Chen E Y,Liao Y C,Smith D H,et al.The human growth hormone locus:nucleotide sequence,biology and evolution.Genomics,1989,4,479-497,
12.Clark SD,Jump DB,Regulation of gene transcriptionby polyunsaturated fatty acids.Prog Lipid Res 1993,32:139-149
13.Decaux J-F,Antoine B,Kahn A,Regulation of the expression of the L-type pyruvate kinase gene in adult rat hepamcytes in primary culture.J Bioi Chem 1989;246:11584-90
14. Dessay E, Wacrenier N, Marche G. Effect of Selection for Improved Body Weight and Composition on Muscle and Meat Characteristics in Muscovy Duck . British Poultry Science, 2002,43 (4) : 560-568.
15. Emilia KOLLAROVA, Zuzana SALAGOVA. Effect of energy intake on lipid profile in pigs. Special Number Proceedings of the International Scientific Conference on the Occasion of the 55th Anniversary of the Slovak Agricultural University in Nitra. 2001, Acta fytotechnica et zootechnica
16. Frahat A, Chavez E R. Effects of line , Dietary protein , sex, age and feed withdrawal on IGF-I in white Peking Ducks[J ] .Poult Sci, 1999, 78 :1307-1312.
17. Freitas, Almir Chalegre de, FUENTES, Maria de Fatima Freire, FREITAS, Ednardo Rodrigues, sucupira, Francislene Silveira, OLIVEIRA, Bruno Cesar Moura de, ESPINDOLA, Gastao, Barreto. Dietary crude protein and metabolizable energy levels for meat quails. Rev Bras Zootecn, 2006,35 (4) : 1705-1710
18. Fu Z W, Tadashi N , Hisanori K. Vitamin A deficiency reduces IGF-I gene expression and increases IGF-I receptor and insulin receptor gene expression in tissues of Japanese quail, J Nutr, 2001,131:1189-1194.
19. Gheisari, A. A., Saraeian, O., Toghiani, M. Response of male broiler breeder chicks to diets containing different levels of energy, protein and sulfur amino acids. J Sci Technol Agric Nat Res, 2005,9 (3) : 185-195
20. Gla-ert HP,Dielary tat,gene expression, and carcino-genesis. In Berdanier CD,Hargrove JL (eds), Nutrition and gene expression.CRC Press,Boca Raton,FL, 1993,247-268
21. Goda T,Bustament S,Koldovsky O.Dietary regulation of intestinal lactase and sucrase in adult rats:quantitative comparison of effect of lactose and sucrose,J Pediatr, Gastroenterol Nutr,1985,4:998-1008;
22. Grobet L,Martin L J,Poncelet D.A deletion in bovine myostatin gene causes the double-muscled phenotype in cattle. Nature Genetics, 1997,17:71:74.
23. Grundy SM,Vega GL,Two different views of the relationship of hypertriglyceridemia to coronary heart disease,implication for treatment,Arch intern Med, 1992,152:28-34
24. Havel RJ,Kane IP,Structure and metabolism of plasma lipoproteins,In Scriver CR,Beaudet AL,Sly WS,Valle D ( eds) ,The meatbolic basis of inherited disease,7th ed,McGraw-Hill,New York,1995,1841-1851
25. Havenstein G B, Ferket P R, Qureshi M A. Growth, livability and feed conversion of 1957 Versus 2001 broilers when fed "typical" 1957 and 2001 broiler diets. Poult Sci, 2003, 82 (10) : 1500-1508
26. Hossain M E, Shahjalal M, Khan M J, Bhuiyan A A. Effect of dietary energy supplementation on feed intake, growth and reproductive performance of sheep under grazing condition. Pakistan J Nutr,2003,2 (3) : 148-152
27. Hu L,Guo DX,Hu R,Liu M,Wang L.Luan XH,Expression rule of MSTN gene in big bones chicken,animal technology, 2003,20 (11), 42-44 (in Chinese). Lauterio TJ ,Scanes CG Journal Nutrition, 1987,117:758 -763.
28. Izevbigi E B, Robbbins K R. Effect of season on metabolizable energy requirement for broiler growth, carcass yield and carcass composition. Tennessee Farm Home Sci, 1988,148:8-12
29. Jenn-Chung Hsu, Ta-Wei LU, Peter W S, Chiou,et al. Effect of different sources of dietary fibre on growth performance and apparent digestibility in geese.Animal Feed Science Technology, 1996,60:93-102
30. Jensen L S, Wyatt C L, Fancher B I. Sulfur amino acid requirement of broiler chicks from 3 to 6 weeks of age. Poult Sci, 1989, 68:163-168
31. Jeroch H, Schubert R, Prinz M, Petzod M. Energy and crude protein requirements of young fattening geese. 2. Influence of varying energy and crude protein contents of feed on carcass quality. Arch Tieremahr, 1978,28 (2) : 111-122
32. Ji S.Myostatin expression in porcine tissues:tissue specificity and developmental and postnatal regulation American Journal of Physiology, 1998,275: R1265-R1273.
33. Jiang S Q, Ding F Y, Lin Y C. Study on requirement of metabolizable energy for 0-21-day-old Lingnan yellow broiler chicks. J South China Agric Univ, 2003,24 (2) : 73-76
34. John P,McMurty. Nutritional and developmental roles of insulin-like growth factors in poultry.Journal of Nutrition, 1998,128 :302S - 305S.
35. Kambadur R,Sharma M,Smith T P.Mutations in myostatin in double-muscled Belgian Blue and Pledmontese cattle .Genome Research, 1997,7 (9) :910-916.
36. Katoh K, Obura YC. Developmental and nutritional astral of somatotropic axis in the ruminant [ J ]. Asian,Aust J Anim Sci, 2001,14 (Special issue ) : 91 -99.
37. Kauffman R G, Carpenter Z L, Bray R W, Hoekstra W G Biochemical properties of pork and their relationship to quality intramuscular fat. J Food Sci, 1963,29: 70-74
38. Kim W K, Kim M H, Seo D S, Lee C Y, Suk Y O, Ko Y. Associations between feed efficiency, body growth and serum insulin-like growth factor-I level for Korean native ogol chickens. Asian Austral JAnim Sci, 2005, 18 (4) : 532-517
39. Kinsell LW,GD MIchael,Letter to editor,Am J Clin Nutr, 1955, 3:247-253
40. Kita K, Ivoda C, Miki K, et al. Relationship of IGF-I mRNA levels to tissue development in chicken embryo of different strains .Asian-Aust J Anim Sci. 2000,13 :1653-1658 .
41. Kita K, Okumuru J. Chicken insulin-like growth factor-I stimulates protein synthesis of chicken embryo myoblasts culture in serum-free medium .Asian-Aust JAnim Sci. 2001,14 :17-20 .
42. Kiyoshi K, Frances B C, Yoshitaka K J, et al. Expression of insulin-like growth factor-I during chicken development .Endocrinology. 2001,142 :1234-1239 .
43. Kocamis H, Kirkpatrick D C, Klandorf H, et al. In Ovo Administration of recombinant human IGF-I alters postnatal growth and development of the broiler chicken .Poult Sci. 1998, 77:1913-1919.
44. Larzul C,Rouvier R,Guy GEstimation of genetic parametersfor growth carcass and over feeding traits in a white goosestrain.Genetics Selection Evolution 2000,32 (4) :415-427.
45. Latorre M A, Lazaro R, Valencia D G, Medel P, Mateos G G The effects of gender and slaughter weight on the growth performance, carcass traits, and meat quality characteristics of heavy pigs. J Anim Sci, 2004, 82 (2) : 526-533
46. Lauterio TJ ,Scanes CG Hormonal responses to protein restriction in two strains of chickens with different growth. characteristics.Journal Nutrition, 1987,117 :758-763.
47. Lee C Y, Lee H P, Jeong J H, et al. Effects of restricted feeding, low-energy diet and implantation of trenbolone acetate plus estriol on growth, carcass traits, and circulating concentrations of insulin-like growth factor ( IGF )-I and (IGF)-binding protein-3 in finishing barrows .J Anim Sci. 2002, 80 :84-93 .
48. Li Q F, Xie Z, Qiang Ba Y Z, et al. Study on the Correlation between Blood Enzyme Activity and Production Performance in Tibetan Yaks. Developmental & Reproductive Biology, 2003,11 (1): 8-12.
49. Life Sciences Research Office.The evaluation of the energy of certain sugar alcohols used as food ingredients.Federation of American Societies for Experimmental Biology,Bethesda,MD. 1994
50. Lilmatta M,Towle HC,Clarke S,Jump DB.Dietary polyunsaturated fatty acids interfere with the insulin/glucose activation of L-type pyruvate kinase gene transcription.Mol Endocrinol 1994; 8:1147-53
51. LIN J,ARNOLD H B,DELLA-FERA M A. Myostatin knockout in mice increases myogenesis and decreases adipogenesis. Biochemical and Biophysical Research Communica- tions.2002,291 (3) .701-706.
52. Liu Z,Thompson KS,Towle HC,Carbohydrate regulation of the rat L-type private kinase gene requires two nuclear factors:LF-Al and a member of the c-myc family.J Biol Chem 1993; 268:12787-95
53. Lu WF.Porcine Myostatin gene Expression and its Location in Different Tissues.Ph D dissertation,Veterinary College of PLA. 2003,11-13.
54. Ma XY, Cao YC, Shu DM,Bi YZ, Clonging and expression of myostatin gene and animal immunity experiment,Science in china series c, 2004,34 (6) :522-526. (in Chinese)
55. Ma XY, Shi ZD, Cao YC, Bi YZ, Ma JY, Cloning and sequencing analysis of myostatin gene of chicken and goose, journal of south china agricultural university (natural science edition) , 2004, 4 (2) ,85-88. (in Chinese)
56. Mazumder N K, Mazumder A. Relational between Plasma Alkalinephospha Tase White Enzyme Activity in White Leghorn Chicken. Indian J Ani Sci, 1991,61 (3) : 299-300.
57. Mcguinness M C, Lreg D W, Balon W L. Effects of factor and refeeding on the somatotropic and thyroidares in Juvenile Chickens . Poult Sci, 1998 , 77 (suppl 1) : 49.
58. McMurtyJP. .J Nutr, 1998 ,128 :302S-305S;
59. Mcpherron A C, Lawler A M, Lee S J. Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member. Nature, 1997,3 87 (6628) :83-89.
60. McPherron A C, Lee S J. Double muscling in cattle due to mutations in the myostatin gene. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94: 12457-12461.
61. Mcpherron A C,LEE S J. Suppression of body fat accumulationin myostatin-deficient mice. The Journal of Clinical Investigation,2002,109 (5) .595-601.
62. Mendes A A, Moreira J, de Oliveira E G. Effect of dietary energy on performance, carcass yield and abdominal fat of broiler chickens. Rev Bras Zootecn, 2004,33 (6) : 2300-2307
63. Mensink RP,Katan MB.Effects of dietary fatty acids on serum lipids and lipoproteins:a meta-analysis of 27 trials.Arteriosclerosis,1992,12:911-919
64. Mignon-Grasteau S, Beaumont C, Le Bihan-Duval E, Poivey JP, De Rochambeau H, Ricard FH.. Genetic parameters of growth curve parameters in male and female chickens. Brit Poult Sci , 1999,40 (1) : 44-51
65. Mohanna C, Nys Y. Effect of dietary zinc content and sources on the growth, body zinc deposition and retention, zinc excretion and immune response in chickens . British Poultry Science, 1999,40 (1) : 108-114.
66. Morrill JS,Kwong LK,Sunshine P,et al. Dietary CHO and stimulation of carbohy- drates along cillus column of fasted rat jejumum,AM J Physiol,1989,256:G158-G165;
67. Nahashon S N, Adefope N, Amenyenu A N, Wright D. Effects of Dietary Metabolizable Energy and Crude Protein Concentrations on Growth Performance and Carcass Characteristics of French Guinea Broilers. Poult Sci, 2005,84 (2) : 337-344
68. Nahashon S N, Adefope N, Amenyenu A, Wright D. Effect of varying metabolizable energy and crude protein concentrations in diets of Pearl Gray guinea fowl pullets 1. Growth performance. Poult Sci, 2006c, 85 (10) : 1847-54
69. Nahashon S N, Aggreg S E, Adefope N A, Amenyenu A. Modeling Growth Characteristics of Meat-Type Guinea Fowl. Poult Sci, 2006b, 85 (5) : 943-946
70. Nahashon S N, Aggrey S E, Adefope N A, Amenyenu A, Wright D. Growth Characteristics of Pearl Gray Guinea Fowl as Predicted by the Richards,Gompertz,and Logistic Models.Poult Sci,2006a,85(2):359-363
71.Nahashon S,Adefope N,Amenyenu A,Lema M,Wright D.Growth and carcass characteristics of French guinea broilers fed diets with varying concentrations of metabolizable energy.J Sustain Agile,2006d,27(4):25-43
72.Nawaz H,Mushtaq T,Yaqoob M.Effect of Varying Levels of Energy and Protein on Live Performance and Carcass Characteristics of Broiler Chicks.Poult Sci,2006,43(4):388-393
73.Nguyen T V,Bunchasak C.Effects of dietary protein and energy on growth performance and carcass characteristics of Betong chicken at early growth stage.Songklanakarin J Sci Technol,2005,27(6):1171-1178
74.NRC编:《家禽营养需要》(第九次修订版),蔡辉益等译,中国农业科技出版社,1994
75.Nutrient Requirements of Poultry,Ninth Revised Edition,1994,National Research Council,1994.
76.Panda A K,Niranjan M,Reddy B L N,Sharma R P.Influence of dietary energy on growth,immunecompetence and carcass characteristics of coloured broiler chickens.Anita Nutr Feed Technol,2006,6(1):115-121
77.Persia M E,Dehority B A,Lilburn M S.The Effects of Enzyme Supplementation of Corn-and Wheat-Based Diets on Nutrient Digestion and Cecal Microbial Populations in Turkeys.Journal of Applied Poultry Research,2002,11(2):134-145.
78.Rausch M I,Tripp M W,Goroni K E.The influence of level of feeding on growth and serum insulin-like growth factor-Ⅰ and insulin-like growth factor binding proteins in growing beef cattle supplemented with somatotropin.J Anim Sci.2002,80:94-100.
79.Ren J B,Zhao G Y,Li Y X,Meng Q X.Influence of dietary lysine level on whole-body protein turnover,plasma IGF-I,GH and insulin concentration in growing pigs.Livest Sci,(2006),doi:10.1016/j.livsci.2006.10.009
80.Rios R,Cameiro I.Myostatin is an inhibitor of myogenic differentiation.American Journal of Physiology Cell Physiology,2002,282(5):C993-999.
81.Roberson R H,Francis D W.The effect of protein level,iodinated casein and supplemental methionine on the performance of White Chinese geese.Poult Sci,1963,42:863
82.Saleh E A,Watkins S E,Waldroup A L,Waldroup P W.Effects of Dietary Nutrient Density on Performance and Carcass Quality of Male Broilers Grown for Further Processing.Int J Poult Sci,2004,3(1):1-10
83.Schubert R,Jeroch H,Hennig A.Studies of the energy and crude protein requirements of young fattening geese.Arch Tierernahr,1977,27(12):720-730
84.Scott M L,Nesheim M C,Young R J.Nutrition of the chicken.3rd ed.New York:Scott and Associates,1982
85. Se-Jin Lee.Alexandra C Mcpherron.Myostatin and the control of skeletal muscle mass.Current Opinion in Genetics & Development, 1999,9:604-607.
86. Semanza GS,Auricchio S.Small intestinal disaccharidases.In Scriver CR, Beauset AL,Sly WS,Valle D (eds) ,The metabolic basis of inherites disease,6th ed. McGraw Hill,New York,1989,2975-2997;
87. Shaoquan, J, RL Losinski, S G Comelius, et al.Myostatin expression in porcine tissues: tissue specificity and developmental and postnatal regulation. American Journal of Physiology 1998,275:R1265.
88. Sharma M,Kambadur R,Matthews K GMyostatin,a transforming growth factor-β superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct. Journal of Cellular Physiology, 1999,180:1 -9.
89. Sonstegard TS,Rohrer GA, Smith TP. Myostatin maps to porcine chromosome 15 by linkage and physical analyses. Anim Genet, 1998,29 (1) : 19-22.
90. Stevenson M.H. Effects of diets of varying energy concentrations on the growth and carcass composition of geese. Brit Poult Sci, 1985,26:493-504
91. Stick C D, Martin J L Baxter R C. Production of IGF-I and its binding protein by breeding cow hepatocytes in primary culture. Endocrinology, 1998,16 (3) : 1094-1101
92. Tall AR.Plasma cholesterol ester transfer protein.J Lipid Res, 1993,34:1255-1274
93. Tesseraud S , Pym R A E, Bihan E L, et al. Response of broilers selected on carcass quality to dietary protein supply :Live performance , muscle , development, and circulating in sulin like growth factors (IGF-Ⅰ and Ⅱ) Poult Sci,2003,82:1011-1016.
94. ThomasM,L angley B,Berry C.Myostatin, a negative regulator of muscle growth, functions by inh ibiting myoblast proliferation. Journal of Biological Chemistry, 2000; 275:40235-40243.
95. Vasilators-Younken R, Scanes C G Growth hormone and insulin-like growth factors in poultry growth: required, optimal, or ineffective. Poult Sci, 1991,70 (8) : 1764-1780
96. Vasilatos2younken R. Absence of growth hormone2induced avian muscle growth in vivo [J ] . Poult Sci, 1999, 78 : 759-768.
97. Vasilatos-younken R. Absence of growth hormone-induced avian muscle growth in vivo .Poult Sci. 1999, 78 :759-768 .
98. Vaulont S,Puzenat N,Levrat F,et al.Proteins binding to the liver-specific pyruvate kinase gene promoter:a unique combination of known factors.J Mol Biol 1989; 209:205-19.
99. Waldroup P W, Tidwell N M, Izat A L. The effect of energy and amino acid levels on performance and carcass quality of male and female broilers grown separately. Poult Sci, 1990,69:1513-1521
100. Wang W J, Huang L S, Chen K F, et al. Polymorphism of insulin-like growth factor-I Gene in 13 pig breeds and its relationship with pig growth and carcass traits .Asian-Aust J Anim Sci. 2002, 15:1391-1394.
101.Whang K Y,Donovan S M,Easter R A.Effects of protein deprivation on subsequent efficiency of dietary protein utilization in finishing pigs.Asian-Aust J Anim Sci.2000,13:659-665.
102.Yab Lanski T.Correlation between the Activities of the Plasma Enzymes GOT,GPT,AKP and Some Performance Qualities in Pigs.Col Sci Works,1986,(30):599-616.
103.Yalcin S,Ozkan S,Acikgoz,Z,Ozkan,K.Influence of dietary energy on bird performance,carcass parts yields and nutrient composition of breast meat of natural optimum and summer temperature.Brit Poult Sci,1998,39(5):633-638
104.Yamada K,Goda T,Bustamente S,Koldovsky O(1983).Different effectof starvation on activity of sucrase and lactase in rat jujunoileum.AM J Physiol 245:G449-G455;
105.Yu B.Effect of Different Sources of Dietary Fiber on Growth Performance,Intestinal Morphology and Caecal Carbohydeases of Domestic Geese.British Poultry Science,1998,39(4):560-567.
106.Zanusso J T,Oliveiva R F M,Donzele J L.Metabolizable energy levels for broilers(1 to 21days) maintained under a thermoneutral enviroment.Rev Bras Zootecn,1999,28(5):1068-1074
107.Zhang KY,Cheng DW.Insulin-like growth factor and its nutritional manipulation,Chinese Journal of Animal Science,2002,38(6),42-44.(in Chinese)
108.Zimrners T A,M V Davies,L G Koniaris,P Haynes,A F ESquela,K N Tomkinson,A C Mcpherron,N M Wolfman,Se-Jin LEE,(2002).Induction of Cachexia in mice by systemically administered myostatin.Science,296(5572):1468-94.
109.曹爱青,毛宗林,胡骏鹏,范卫星,何瑞国,不同能量水平对朗德鹅生长曲线的影响研究,饲料工业,2007第28卷第7期,34-37.
110.陈国宏,卞建春,王克华等.地方鸡种血浆AKP和GPT及CPK活力比较研究.西北农业大学学报,1998,26(4):66-70.
111.陈国宏.中国部分地方鸡种肉品质性状比较研究.[博士学位论文].杨凌:西北农业大学,1997
112.陈继兰,吕连山,赵玲,方丽.石歧黄肉鸡前期日粮适宜的能量和蛋白质水平的研究.中国畜牧杂志,1998,34(4):10-12
113.陈金文,杨山,莫棣华,文伯珍,赵河山,胡刚安.日粮能量和蛋白水平对肉鸡腹脂和血脂的影响.动物营养学报,1998,10(1):20-28
114.陈耀王.高档营养食品鹅肥肝的生产.科学技术文献出版社,北京.1987.
115.程红娜.合浦鹅饲粮能量、粗蛋白质、钙和磷适宜水平的研究.[硕士学位论文].南宁:广西大学,2005.
116.邓兴照,彭华,刘福柱.类胰岛素生长因子-Ⅰ及其营养调控,中国畜牧杂志,2005,41(3),39-41.
117.杜卫国,鲍毅新,施利强,俞华英.社鼠内脏器官重量和水分含量的季节性变化.动物学杂志.1999,34(1):23-25.
118.范卫星,何瑞国,胡骏鹏,曹爱青,朗德鹅血液性状与体脂沉积间相关关系的研究,饲料工业,2007第28卷第15期,36-38.
119.范卫星,何瑞国,胡骏鹏,曹爱青等,日粮能量水平对超饲养前溆浦鹅脂肪代谢的影响研究,中国饲料,2007第13期,18-21.
120.方立超.不同的饲粮能量和蛋白质水平对肉鸡肉质及痛风症发生率的影响.[硕士学位论文].重庆:西南农业大学,2001.
121.房兴堂,王景明,雷丛,张海琪,刘玉梅,孙刚,王立强.肉鸡消化器官生长规律的研究.畜禽业,1999(1):12-12.
122.房兴堂,王景明,雷丛,张海琪,刘玉梅.肉鸡内脏器官生长规律的初步探讨.中国家禽,1998,20(6):10-12.
123.付予华,刘琦山,荣雷,李吕木,吴剑平,张明生.不同能蛋水平日粮对皖西白鹅早期增重的试验.中国家禽,1991(5):29-31.
124.高波.新太湖鹅不同世代肉用性能改进效果及RAPD和活性研究.[硕士学位论文].扬州:扬州大学,2000.
125.葛剑,谷子林,李英,魏忠华,郑常山.河北柴鸡1-16周龄生长曲线分析与拟合的比较研究.中国家禽,2005,27(14):9-11.
126.呙于明.家禽营养[M].北京:中国农业大学出版社.1997,19-20.
127.郝正里,肖金玉,项光华等.维生素A、D、E添加水平对产蛋鸡血清与蛋黄生化指标的影响.甘肃农业大学学报,2000,35(3):243-250.
128.何瑞国,刘祥友,李翔,赵阿勇,赵娜,张巍,周樱.坚持科学发展观,持续发展湖北水禽业.中国农学通报,2005,21(增刊):210-213.
129.候曼玲.食品分析.化学工业出版社.北京.2004.5.
130.胡骏鹏,何瑞国,范卫星,曹爱青等,豆油能量对朗德鹅超饲养前期生长性能,血清参数及其相关激素水平的影响研究,中国粮油学报(待发).
131.胡骏鹏,何瑞国,范卫星,曹爱青等溆浦鹅肌肉生成抑制因子(MSTN)基因的克隆及其表达量与日粮能量,血清IGF-I和GH的关系研究,畜牧兽医学报(待发).
132.胡骏鹏,李翔,范卫星,曹爱青,赵阿勇,何瑞国等,朗德鹅肌肉生成抑制因子(MSTN)基因的克隆及其表达量与日粮能量,血清IGF-I和GH的关系研究,中国农业科学(待发).
133.胡兰,郭东新,胡锐,刘梅,王娜,栾新红.大骨鸡中MSTN基因表达规律性的研究.动物科技,2003年第20卷第11期,42-44.
134.黄建亮.江山白鹅早期生长发育特性的研究.中国草食动物,2000,2(4):20-21.
135.蒋守群,丁发源,林映才,余德谦,杨晓建.能量水平对0-21日龄岭南黄鸡生产性能、胴体品质和体组成的影响.中国家禽学报,2003,7(1):83-86.
136.井文倩.三类型黄羽肉鸡适宜能量水平的比较研究.[硕士学位论文].泰安:山东农业大学,2002
137.孔保华等,肉制品工艺学.黑龙江科学技术出版社,哈尔滨.1996.
138.李福昌,王悦忠,颜炳军.AA肉仔鸡肌肉酸度的测定报告.中国家禽,1993,(5):24-25.
139.李俊波,左绍群,张克英.生长前期丝羽乌骨鸡饲料适宜能量、蛋白水平研究.动物营养学报,2000,12(3):37-43.
140.李文立,王宝维,刘光磊,等.不同钙磷水平对0-4周龄五龙鹅生产性能及血浆和胫骨生化指标的影响.动物营养学报,2004,17.
141.李文立,王宝维,刘光磊,等.不同钙磷水平对5-8周龄五龙鹅生产性能及血浆和胫骨生化指标的影响.中国农学通报,2005,21(3):15-19.
142.李翔,糙米型饲粮对鹅鸭肥肝性能影响及朗德鹅肥肝脂肪沉积规律的研究,华中农业大学2005届博士论文,12-16.
143.廖玉英,杨家晃,何仁春,秦黎梅,麦伟虹,卢桂猷.日粮代谢能水平对鹅生长性能、养分利用率及肉质的影响.粮食与饲料工业,2006(5):38-41.
144.林树茂等,不同禽肉脂肪酸组成的比较研究.中国畜牧杂志,2004,40(12):18-20.
145.刘定发,柳小春,施启顺,陈斌,王治平.番鸭血浆酶活性及其与产肉性能关系的研究.当代畜牧,1999,(2):41-42.
146.刘建文,施用晖,乐国伟.动物生长轴的激素调控.中国饲料,2003,(14):7-9.
147.刘思扬.肉用狮头鹅技术产量指标测试初报.广东畜牧兽医杂志,2000,9(25):3.
148.刘翔友,何瑞国等,日粮粗纤维对朗德鹅生产性能和血清参数影响的研究,中国粮油学报,2006,6,21(3),178-182.
149.刘雨龙,刘惠兰.蛋鸡育成阶段内脏器官的生长分析.饲料与畜牧,1995,(1):9-12.
150.刘玉兰,冯定远.性别对生长肥育猪生长性能、胴体品质和肌肉营养成分的影响.粮食与饲料工业,2005,(3):39-41.
151.卢德勋.系统动物营养学导论.北京:中国农业出版社出版,2004.
152.吕明斌,黄启贤.日粮添加大豆油对肉用仔鸡的饲喂效果.中国饲料,1997,(12):17-18.
153.吕文发.猪肌生成抑制素(Myostatin,MSTN)表达及组织定位研究,博士学位论文,中国人民解放军军需大学,2003,11-13.
154.罗庆斌,何大乾,尹荣楷,龚绍明,孙国荣.我国养鹅业现状及发展趋势.中国家禽,2006,28(4):1-4.
155.麻艳群.饲粮营养水平、纤维来源对鹅利用纤维潜力和生产性能的影响.[硕士学位论文].南宁:广西大学,2005.
156.马国龙,孙鹏翔.肉用中型仔鹅能量和蛋白质需要量的研究.水禽世界,2006,(2):33-37.
157.马现永,曹永长,舒鼎铭,毕英佐,肌肉生成抑制因子基因的克隆、表达及动物免疫实验,中国科学,C辑,2004,34(6):522-526.
158.马现永,施振旦,曹永长,毕英佐,马静云.鸡、鹅肌肉生成抑制因子基因的克隆和序列分析,华南农业大学学报(自然科学版)2004年4月第25卷第2期,85-88.
159.毛国祥,陈柯,赵万里.隆昌鹅、太湖鹅及新太湖鹅部分血液生化指标研究.动物科学与动物医 学2000,17,(5):18-20.
160.毛国祥,赵万里.太湖鹅、隆昌鹅及新太湖鹅生长规律研究.动物科学与动物医学,2001,18(2):11-13.
161.毛国祥,赵万里.新太湖鹅,太湖鹅和隆昌鹅肌肉品质比较研究.动物科学和动物医学,2000,17(1):16-19.
162.米玉玲,王安.低温高能饲粮对笼养产蛋鸭生产性能及生化指标的影响.动物营养学报,2003,15(1):21-25.
163.闵育娜,侯水生,高玉鹏,黄苇,赵玲,喻俊英.0-4周龄肉仔鹅能量和蛋白质需要的研究.黑龙江畜牧兽医,2005a(6):26-27.
164.闵育娜,侯水生,高玉鹏,黄苇,赵玲,喻俊英.日粮能量蛋白水平对肉仔鹅胴体性能和血液生化指标的影响.西北农林科技大学学报(自然科学版),2005b,33(6):40-44.
165.莫棣华.对“广黄”鸡的能量和蛋白质需要的研究.广东农业科学学,1983,(3):27-30.
166.欧茶海等.不同日龄和性别腾冲雪鸡肉的营养成分分析.西南民族学院学报,1999,25(2):181-186.
167.欧阳克蕙,王文君,瞿明仁,范玉庆.油脂对泰和乌骨鸡生长后期生产性能的影响.江西饲料,2002(6):9-10.
168.祁永红.扬州鹅早期生长发育规律及其肉用性能的研究.[硕士学位论文].扬州:扬州大学,2001.
169.卿晓红等,三穗鸭肉、蛋及羽毛粉营养成分含量分析.贵州畜牧兽医,1996,20(5):11-12.
170.邱祥聘,周世永.丝羽乌骨鸡肉品质的研究概况.畜禽业,2002(7):8-9.
171.佘德勇.北京鸭和樱桃谷鸭生长性能、肌肉理化特性比较及填饲对其影响.[硕士学位论文].北京:中国农业大学,2005.
172.沈元新.绍鸭发育与营养研究.成都:成都科技大学出版社,1997:57-58.
173.盛志廉,吴常信.数量遗传学.北京:中国农业出版社,1995:104.
174.盛志廉,吴常信.数量遗传学.北京:中国农业出版社,1995:114-115.
175.宋成义,经荣斌.畜禽GH基因多态性与生产性能相关研究进展,国外畜牧科技,2000,27(2),25-27.
176.苏秀侠,张源久,于秀芳,生群.肉仔鹅日粮能量、蛋白和纤维水平的探讨.四川畜禽,1997(7):41-44.
177.孙逊,朱尚权.生长激素的结构与功能,国外医学-生理,病理科学与临床分册,1999,19(1):6-9.
178.孙永峰,陈少康,吴伟.肉用品系鹅生长发育与性状间相关分析.吉林畜牧兽医,2003,8:1-3.
179.王磊,王康宁.影响胃肠道发育因素的研究进展.饲料工业,2006,27(5):13-16.
180.王宝维,刘光磊,张名爱,等.五龙鹅快长系早期生长规律与酶活性关系.西南农业大学学报(自然科学版),2005,27(1):102-106.
181.王宝维,潘庆杰,朱新产,等.“五龙鹅”品种选育与研究进展.中国家禽,2002,24(18):28-32.
182.王宝维,王雷,贾晓晖等.五龙鹅早期生长发育与血清酶活性的关系,甘肃农业大学学报,2005,6 (3):297-301.
183.王秉栋.动物性食品卫生理化检验.中国农业出版社,北京,1994.
184.王林,陈光伟,吴信生,杨凤萍,王金玉,林大光,孔佩兰.肉兔血浆AKP、GPT活性与生产性能关系的研究.畜禽业,2003,(8):12-11.
185.王绮,张晓娜.胰岛素样生长因子的分泌及功能对人体的影响.临床荟萃,2006,21(12):900-901.
186.王淑萍,杨连玉,吴伟.日粮营养水平对雏鹅饲喂效果观察.吉林畜牧兽医,2002(5):1-3.
187.王彦文,宋志琪.日粮能蛋水平对肉仔鸡生产性能的影响.甘肃畜牧兽医,1996,26(1):1-4.
188.王阳铭,王琳,杨文清,宋金昌,李绶章.肉用仔鹅集约化饲养条件下的能量和蛋白质需要.西南农业学报,1999,12(2):103-111.
189.王元林,袁锦和,杨震,等.江苏太湖鹅产蛋期若干生理生化指标的测定(初报).南京农学院报,1983,(3):97-99.
190.王志耕等.皖西白鹅肉脂特性研究.畜牧兽医学报,2002,33(4):332-335.
191.王志跃,陈伟亮,白群安,王健,王欢莉.新扬州鸡生长模型的比较研究.黑龙江畜牧兽医,2002,(12):4-6.
192.闻芝梅,陈君石主译,《现代营养学》(第七版),人民卫生出版社,1998年4月第一版,44-46:49-58.
193.乌尼尔夫,芒来.动物生长激素的研究现状.畜牧与饲料科学,2005,5:37-39.
194.吴东,夏伦志,周明,等.中草药饲料添加剂对种鹅繁殖性能和血清生化指标的影响.粮食与饲料工业,2005,6:41-43.
195.吴信生,杨凤萍,王金玉,林大光,孔佩兰,张振华,沈幼章,翟频,娄志荣,朱满兴,朱瑾佳,潘雨来.肉兔血浆碱性磷酸酶活性比较及其与生产性能的研究.中国养兔杂志,2000,(6):14-16.
196.夏中生,麻艳群,杨家晃等.饲粮纤维来源对合浦鹅生产性能、血清生化指标和养分代谢的影响.畜牧与兽医,2006,38(7):7-10.
197.谢广富.鹅肉营养成分分析及营养价值评定,中国家禽,1999(4).
198.谢庄,杨茂成.不同杂交组合鹅生长规律的比较.南京农业大学学报,1994,17(1):83-87.
199.徐明生,欧阳克蕙,上官新晨,王文君,陈锦屏.油脂对泰和乌鸡生产性能和肌肉脂肪酸组成的影响.福建农林大学学报(自然科学版),2006,35(1):67-72.
200.许盛海,包文斌,程金花,黄军,张红霞,陈国宏.鸭生长激素(GH)基因编码区及调控区多态性分析,畜牧兽医学报,2007,38(9):907-912.
201.许盛海,陈国宏.鸡生长激素基因的研究进展,上海畜牧兽医通讯,2006,3:18-20.
202.颜炳学,邓学梅,费箐,胡晓湘,吴常信,李宁.鸡生长激素基因单核苷酸多态与生长及屠体性状的相关性,科学通报,2003,6,48(2):1304-1307.
203.杨凤.动物营养学(第二版).北京:中国农业出版社,2002.
204.杨光荣,李翠蓉.鹅血浆某些生化指标的测定.畜牧与兽医学报,2004,36(10):29.
205.杨加豹,张亚平,李定发,邓发强,王利琴.日粮能量水平对肉鸭生产性能影响及“标准曲线法”测 定饲用酶制剂的表观代谢能值.中国饲料,2004,(19):12-12.
206.杨凌,葛建东.不同能量水平对四季鹅育肥效果的影响.畜牧与兽医,1998,30(2):67.
207.杨宁.家禽生产学.北京:中国农业出版社.2002,289-292.
208.杨山,家禽生产学,中国农业出版社,1995.
209.叶绍辉,张华英,段纲,欧茶海,李润泉,丁自柏.60-90日龄不同性别腾冲雪鸡血液生化指标差异研究.云南农业大学学报,1999,14(4):345-348.
210.余斌.赖氨酸铜对仔猪生长的影响及其作用机理研究.广州:华南农业大学学位论文,2002.
211.俞照正,陈维虎,王亚琴,等.8周龄浙东白鹅体重体尺和免疫器官的发育研究.中国草食动物,2005,25(2):23-24.
212.张碧丽.生长激素与肾脏,国外医学-内分泌分册,1997,17(2):94-96.
213.张春雷,刘福柱,侯水生.育雏期不同能量蛋白质水平对肉鹅生产性能影响.中国饲料,2004,(18):24-25.
214.张红,龚道清,张军,万建洪,狄志钢,郑建高,谭国才.溧阳鸡生长曲线分析与拟合的研究.畜牧与兽医,2006,38(2):7-9.
215.张克英,陈代文.类胰岛素生长因子及其营养调控,中国畜牧杂志,2002年第38期第6卷,42-44.
216.张喜武.中国水禽业发展现状及对策.中国家禽,2005,27(10):1-3.
217.张显花.中草药饲料添加剂对仔猪生长性能和日粮养分消化率的影响.[硕士学位论文].武汉:华中农业大学图书馆,2005.
218.赵阿勇.朗德鹅对能量蛋白质和钙营养需要的研究.[硕士学位论文].武汉:华中农业大学图书馆,2004.
219.赵向红.有效温度与日粮能量及蛋白水平对肉鸡消化功能的影响.[硕士学位论文].北京:中国农业大学,2005.
220.中国预防医学科学院,营养与卫生研究所,食品成分表.1991.
221.周光宏.肉品学.北京:中国农业科技出版社.1998.
222.周桂莲,林映才,蒋守群,丁发源,郭吉余,余德谦,杨晓健.饲粮代谢能水平对22-42日龄黄羽肉鸡生长性能、胴体品质以及部分血液生化指标影响的研究.饲料工业,2004,25(3):35-38.
223.周洪松,赵益贤.根据雏鸡血清酶活性对蛋鸡进行早期选择的研究[J].畜牧兽医学报,1991,22(3):220-224.
224.毕英左,傅伟龙.动物科学进展.北京:中国农业科技出版社,2000.
225.朱志明,强巴央宗,朱猛进,纪素玲,唐晓惠,莫德林,刘榜.藏鸡生长曲线拟合和分析的比较研究.中国农业科学,2006,39(10):2159-2162.