摘要
以7个水稻三系雄性不育系(华37A、T98A、Ⅱ-32A、V20A、金23A、优ⅠA、农富A)和7个两系不育系(株1S、陆18S、C815S、Y58S、龙S、Z9S、培矮64S)为材料,对花器性状和柱头外露率进行了比较研究,并作了花器性状与柱头外露率的相关性和通径分析。主要结果如下:
(1)颖花性状:14个不育系的颖花开张时间以Ⅱ-32A的为最长,达6.37h,培矮64S和Y58S较短,分别只有1.83h和1.36h。张颖角度最大的是T98A,为42.89°,其它不育系的张颖角度也都比较大,均在35°以上。
(2)柱头性状:T98A和Y58S的柱头长度最长,均为1.97mm、农富A和V20A较短分别为1.58mm和1.54 mm;T98A的柱头宽度最大,为0.84mm,农富A和Z9S较窄,分别为0.64mm和0.52mm;陆18S柱头跨度最大,为3.60mm,华37A和T98A较小,分别为2.91mm和2.88 mm;柱头面积和柱头体积最大的是T98A,农富A和Z9S较小。
(3)柱头外露率及逐日变化:柱头总外露率最高的是Y58S,为89.41%,其它不育系柱头外露率从高至低依次为培矮64S、T98A、农富A、Z9S、龙S、株1S、C815S、华37A、陆18S、优ⅠA、金23A、Ⅱ-32A、V20A。14个不育系的柱头外露率都有随着开花天数的增加而逐渐下降的趋势。
(4)对花器性状与柱头外露率的相关性和通径分析结果表明,柱头外露率与柱头跨度、颖花长、颖花长宽比和子房长度的相关系数分别为0.7848、0.5246、0.6307、0.6165,都达到了极显著水平,其它性状与柱头外露率相关但不显著。颖花长宽比对柱头外露率的直接通径系数最大,为1.5366;柱头跨度对柱头外露率的直接通径系数为0.6204;子房长对柱头外露率的直接通径系数为0.0573;颖花长对柱头外露率的直接通径系数为-0.9847,颖花长是通过影响颖花长宽比对柱头外露率产生影响。
Floral characters,exserted stigmas percentage of 7 three-line MS lines(hua37A, T98A,Ⅱ-32A,V20A,jin23A,youⅠA,longfuA) and 7 two-line MS lines(zhu1S, lu18S,C815S,Y58S,longS,Z9S,peiai64S) and the correlation and path analysis of floral characters related to exserted stigmas percentage.The main results showed:
(1) Spikelet traits.Spikelet opening time ofⅡ-32A(4.18h) was longest. peiai64S(1.83h) and Y58S(1.36h) were short.Opening angle of spikelet of T98A(42.89°) was biggest,the other are all beyond 35°.
(2) Floral characters.Stigma length of T98A and Y58S(1.97 mm) were longest. longfuA(1.58 mm) and V20A(1.54 mm) were short.Stigma width of T98A were widest(0.84 mm).longfuA(0.64 mm) and Z9S(0.52 mm) were narrow.Stigma span of lu18S(3.60 mm) were biggest.Hua37A(2.91 mm) and T98A(2.88 mm) were small.Stigma area and volume of T98A were biggest,longfuA and V20A were small.
(3) Exserted stigma percentage and variety per day.Exserted stigma percentage of Y58S(89.41%),the order of other MS lines in the sequence of descending was peiai64S,T98A,longfuA,Z9S,longS,lu1S,C815S,hua37A,lu18S,youⅠA,jin23A,Ⅱ-32 A,V20A.The exserted stigma percentage of 14 MS lines all descend with the day by day.
(4) The correlation and path analysis of floral characters related to exserted stigma percentage showed that stigma span,spikelet length,length-width ratio of spikelet and ovary length to exserted stigma percentage,their correlation coefficient was 0.7848,0.5246,0.6307 and 0.6165,respectively.The correlation of exserted stigma percentage and other traits had no significant.The direct effect of length-width ratio of spikelet to exserted stigma percentage was the biggest.The path coefficient was 1.5366.The path coefficient of stigma span to exserted stigma percentage was 0.6204.The path coefficient of ovary length to exserted stigma percentage was 0.0573. The path coefficient of spikelet length to exserted stigma percentage was -0.9847.This results showed that spikelet length mainly through the impact of length-width ratio of spikelet exposed to exserted stigma percentage impact.
引文
[1]李稳香,田全国.种子生产原理与技术[M].北京:中国农业出版社,2005:38-108.
[2]许世觉.中国杂交水稻制种技术的发展[J].杂交水稻,1994(3-4):50-51,57.
[3]童海军,马华升.提高杂交水稻制种异交结实率技术[J].中国稻米,1997,(5):14-17.
[4]龙俐华,舒琨.提高杂交稻异交率研究[J].湖南农业大学学报,2000,26(3):167-170.
[5]闵绍楷,申宗坦,熊振民.水稻高产育种[M].北京:中国农业出版杜,1990.
[6]刘平.进一步提高水稻不育系柱头外露率的途径[J].杂交水稻,1998,13(3):33-4.
[7]刘平,祖同梅.提高水稻不育系柱头外露率的栽培技术[J].安徽农学通报,1998,4(2):59-60.
[8]陈稚,曹文元,谢小华.提高杂交水稻制种母本柱头外露率的技术措施[J].杂交水稻,2006,21(3):31-32.
[9]南存枢,叶杰林.怎样提高杂交水稻制种母本柱头外露率[J].温州农业科技,1999,(2):32-33.
[10]田大成,黄三奎,段永国,王友红.水稻不育系花时和受粉时间与异交结实率的关系[J].杂交水稻,2004,(3):50-54.
[11]许克农,李泽炳,李成荃.光(温)敏核不育水稻的育性和开花习性研究[J].安徽农业科学,1992,20(4):293-301.
[12]贺浩华,彭小松,余秋英,等.水稻光温敏核不育系的开花习性观察[J].中国水稻科学,1998,12(4):245-248.
[13]田大成.水稻异交栽培学[M].成都:四川科学技术出版社,1991:70-83.
[14]田大成,张素英,秦春林.提高柱头外露率是杂交稻制种稳产高产的关键措施[J].四川农业科技,1990,(2):16-18.
[15]魏东.影响杂交水稻制种产量的制约因素及提高异交结实率的技术途径[J].种子,2002,(5):84-86.
[16]沈和森.提高杂交水稻制种异交结实率探讨[J].种子,1995,(1):38-39.
[17]林建荣,吴明国,宋昕蔚.三系粳稻不育系开花习性与异交结实率的关系[J].杂交水稻,2006,21(5):69-72.
[18]杨保汉.不育系柱头外露率及其结实率研究[J].杂交水稻,1997,12(1):13-15.
[19]李庆荣.柱头外露率的环境变异及其在异交结实中的作用[J].种子科技,1995,(2):32-33.
[20]邓秋生.保花肥对母本柱头外露及结实率的影响[J].杂交水稻,1995,(1):13-14,37.
[21]吕凯,魏凤娟,吴永辉,等.施肥和激素对水稻不育系柱头外露率和结实率的影响[J].安徽农业科学,2003,31(4):641-642.
[22]田大成,秦春林,张素英,等.母本柱头外露率与环境条件及结实率关系的初步研究[J].杂交水稻,1990,(3):17-20.
[23]邓应德,唐道传,郭名奇,等.“九二○”对水稻不育系异交结实率的影响及其原因分析[J].杂交水稻,1998,13(2):38-39.
[24]李陶,陈一吾.水稻柱头外露率的遗传分析[J].作物学报,1987,13(40):314-321.
[25]严文贵.几个水稻同核异质雄性不育系的比较研究[J].四川农业大学学报,1985,3(2):63-72.
[26]严文贵.几个水稻同核异质不育系的异交习性研究[J].杂交水稻,1987,(4):8-11.
[27]杨仁崔,卢浩然.稻属(Oryza L.)的核质互作效应研究[J].中国农业科学,1989,22(2):56-63.
[28]王文明,周开达,文宏灿,等.水稻异交性状的质核互作分析[J].四川农业大学学报,1995,13(4):451-455.
[29]张静,陈国荣,黄大军,等.滇型杂交粳稻保持系与不育系柱头外露的遗传关系[J].云南农业大学学报,2005,(4):459-461.
[30]凌祖铭.水稻柱头外露性状的研究,Ⅱ.水稻柱头外露性状的遗传研究[J].北京农业大学学报,1989,(01):23-27.
[31]张戟,陆建国,盛金元,等.籼粳交后代柱头外露率的遗传表现与选择[J].江苏农业科学.2000,(02):6-9.
[32]Virmani S S,Athwal D S.Inheritance of floral characteristic influencing out-crossing in rice[J].Crop Sci.,1974,14:350-353.
[33]Hassan M A,Siddiq E A.In heritance of anther size and stigma exsertion in rice[J].IndianJGenet,1984,44(3):544547.
[34]Yang RC(杨仁崔),Lu HR(卢浩然).Genetic studies on floral characters of Chinese indica early-varieties in rice[A].In:Hunan Hybrid Rice Research Center(HHRRC).Proceedings of International Symposium on HybridRice,6-10 Octoberl986,Changsha,China[C].Peking:Scientific Periodical Press,1986.357-360.
[35]喻婷,张玲,胡中立,等.水稻籼粳交DH群体花器性状的遗传分析[J].武汉植物学研究.2003,(6):459-463
[36]Virmani S S,Athwal D S.Genetic variability in floral characteristic influencing out-crossing in Oryza sativa L.[J].Crop Sci,1973,13:66-67.
[37]Yirmani S S.Heterosis and HybridRice Breeding[M].Berlin Heideberg NewYork:Spring-verlag,1994.
[38]NanaiH,KatoH.Improving pollination characteristics of japonica rice[A].In:IRRI ed.Hybrid Rice.Proceedings of the International Symposium[C].Manila:IRRI,1988.165-173.
[39]Parmar K S,Siddiq E A,Swaminathan M S.Variation in components of flowering behavior of rice[J].Indian J Genet,1979,39(3):542-550.
[40]Parmar K S,Siddiq E A,Swaminathan M S.Variation in anther and stigma characteristics in rice[J].Indian J Genet,1979,39(3):551-559.
[41]武小金,袁隆平.温敏核不育水稻柱头外露率的群体改良研究[J].作物学报,1998,24(1):68-73.
[42]Keddie J S,Carroll B J,Thomas C M,et al.Plant Cell,1998,10(6):877-888.
[43]杨仁崔.水稻花器性状的配合力研究[J].作物学报,1987,13(3):223-227.
[44]孙传清,王象坤,吉村淳,等.普通野生稻和亚洲栽培稻遗传多样性的研究[J].遗传学报,2000,27(3):227-234.
[45]Xiao J H,Grandillo S,Sang N A,et al.Genes from wild rice improve yield[J].Nature,1996,384:223-224.
[46]李晨,孙传清,穆平,等.花药长度和柱头外露率的QTL定位[J].遗传学报,2001,28(8):746-751.
[47]王象坤,程侃声,黄迈威,等.亚洲栽培稻起源演化中两个重要稻种类型的研究[J].遗传学报,1987,14(4):262-270.
[48]陈立云.两系法杂交水稻的理论与技术[M].上海:上海科学技术出版社,2001:126.
[49]李文宏,董国军,胡新民,等.水稻柱头外露率的QTL分析[J].遗传学报,2003,30(7):637-640.
[50]沈圣泉,庄杰云,包劲松,等.水稻柱头外露率QTL s定位及其互作分析[J].生物数学学报,2006,21(4):610-614.
[51]徐建龙,薛庆中,梅捍卫,等.水稻数量性状位点(QTL)定位研究进展[J].浙江农业学报,2001,13(5):315-321.
[52]何平,李仕贵,李晶昭,等.影响稻米品质几个性状的基因座位分析[J].科学通报,1998,43(16):1747-1750.
[53]王鸿昌,梁克勤,万邦惠,等.水稻光温敏核不育系培矮64S的开花习性观察[J].杂交水稻,1995,(3):12-14.
[54]肖层林,李仕贵,李晶昭,等.水稻籼型温敏不育系培矮64S异交特性研究[J].杂交水稻,2000,(15):59-61.