舒肝安神方治疗失眠的实验研究
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摘要
目的:研究舒肝安神方对小鼠睡眠的影响。
方法:健康昆明小鼠120只,随机分为三大组。第一组40只随机分成空白对照组、艾司唑仑组、舒肝安神方大剂量组、舒肝安神方小剂量组,各组分别给予等体积蒸馏水、艾司唑仑、舒肝安神方大剂量和小剂量灌胃,连续给药7天,观察舒肝安神方对小鼠自主活动的影响,在完成对自主活动影响的观察后,取血检查血生化指标,然后处死,取肝脏做病理切片。第二组40只按上述方法分组并分别给药7天后,予以戊巴比妥钠阈上剂量腹腔注射,观察舒肝安神方对小鼠睡眠潜伏期和睡眠持续时间的影响。第三组40只健康昆明小鼠按上述方法分组并分别给药7天后,予以戊巴比妥钠阈下剂量腹腔注射,观察舒肝安神方对小鼠入睡率的影响。
结果:舒肝安神方与艾司唑仑均能减少小鼠的自主活动,舒肝安神方大小剂量之间无显著差异;舒肝安神方和艾司唑仑均能显著缩短小鼠的睡眠潜伏期、延长小鼠的睡眠持续时间;艾司唑仑、舒肝安神大剂量均能显著增加小鼠的入睡率,舒肝安神方与艾司唑仑之间无显著差异;未发现舒肝安神方对小鼠的生理生化指标的影响和小鼠肝脏的毒性作用。
结论:舒肝安神方可明显降低小鼠的自主活动、缩短睡眠潜伏期、延长睡眠持续时间、提高入睡率、对小鼠睡眠有明显的促进作用,对小鼠的生理生化指标无影响,对小鼠肝脏组织无毒性作用。舒肝安神方是治疗失眠的有效方剂,具有明显的镇静催眠效果。
Objective:To study the effect of prescription of ShuGanAnShen for the rats of sedative and hypnosis.
Materials and Methods:120 Kun Ming healthy rats were evenly divided into three groups randomly. 40 rats in the first group were evenly divided into four groups randomly: control group, estazolam group, large dose of ShuGanAnShen group and small dose of ShuGanAnShen group. Rats in four groups were perfused by the same volume of distilled water, estazolam solution, large dose of ShuGanAnShen decoction, small dose of ShuGanAnShen decoction respectively. After seven days, each group was observed respectively to learn the prescription effect in aspect of autonomic activities. After this procedure, blood was taken from the rats in large dose of ShuGanAnShen group for biochemical assay. Then the rats were killed in order to get liver biopsy. 40 rats in the second group were grouped and irrigated medicine as the first group. Seven days later, the rats were perfused sodium pentobarbital above the threshold by celiac cavity and observed the effect of ShuGanAnShen in sleep latent time, sleep duration. The third group was as the same as the second group except that sodium pentobarbital were given subthreshold.
Results:The prescription of ShuGanAnShen and Estazolam both could reduce the frequency of rats’autonomic activities,there was no significant difference between large and low dose group of ShuGanAnShen. The two drugs could also apparently decrease sleeping incubation period and prolong sleep duration. Estazolam and large dose group of ShuGanAnShen could increase the rate of falling asleep and there was no significant difference between the two groups. No effect of biochemical indexes and toxicity of liver of rats were discovered during the treatment by prescription of ShuGanAnShen.
Conclusions:Prescription of ShuGanAnShen could decrease the frequency of rats’ autonomic activities and period of falling asleep. It could also prolong sleeping time and increase the rate of falling asleep of rats. The prescription had an obviously effect on sleeping and no effect on liver and biochemical indexes .This prescription had effective treatments on insomnia.
方法:健康昆明小鼠120只,随机分为三大组。第一组40只随机分成空白对照组、艾司唑仑组、舒肝安神方大剂量组、舒肝安神方小剂量组,各组分别给予等体积蒸馏水、艾司唑仑、舒肝安神方大剂量和小剂量灌胃,连续给药7天,观察舒肝安神方对小鼠自主活动的影响,在完成对自主活动影响的观察后,取血检查血生化指标,然后处死,取肝脏做病理切片。第二组40只按上述方法分组并分别给药7天后,予以戊巴比妥钠阈上剂量腹腔注射,观察舒肝安神方对小鼠睡眠潜伏期和睡眠持续时间的影响。第三组40只健康昆明小鼠按上述方法分组并分别给药7天后,予以戊巴比妥钠阈下剂量腹腔注射,观察舒肝安神方对小鼠入睡率的影响。
结果:舒肝安神方与艾司唑仑均能减少小鼠的自主活动,舒肝安神方大小剂量之间无显著差异;舒肝安神方和艾司唑仑均能显著缩短小鼠的睡眠潜伏期、延长小鼠的睡眠持续时间;艾司唑仑、舒肝安神大剂量均能显著增加小鼠的入睡率,舒肝安神方与艾司唑仑之间无显著差异;未发现舒肝安神方对小鼠的生理生化指标的影响和小鼠肝脏的毒性作用。
结论:舒肝安神方可明显降低小鼠的自主活动、缩短睡眠潜伏期、延长睡眠持续时间、提高入睡率、对小鼠睡眠有明显的促进作用,对小鼠的生理生化指标无影响,对小鼠肝脏组织无毒性作用。舒肝安神方是治疗失眠的有效方剂,具有明显的镇静催眠效果。
Objective:To study the effect of prescription of ShuGanAnShen for the rats of sedative and hypnosis.
Materials and Methods:120 Kun Ming healthy rats were evenly divided into three groups randomly. 40 rats in the first group were evenly divided into four groups randomly: control group, estazolam group, large dose of ShuGanAnShen group and small dose of ShuGanAnShen group. Rats in four groups were perfused by the same volume of distilled water, estazolam solution, large dose of ShuGanAnShen decoction, small dose of ShuGanAnShen decoction respectively. After seven days, each group was observed respectively to learn the prescription effect in aspect of autonomic activities. After this procedure, blood was taken from the rats in large dose of ShuGanAnShen group for biochemical assay. Then the rats were killed in order to get liver biopsy. 40 rats in the second group were grouped and irrigated medicine as the first group. Seven days later, the rats were perfused sodium pentobarbital above the threshold by celiac cavity and observed the effect of ShuGanAnShen in sleep latent time, sleep duration. The third group was as the same as the second group except that sodium pentobarbital were given subthreshold.
Results:The prescription of ShuGanAnShen and Estazolam both could reduce the frequency of rats’autonomic activities,there was no significant difference between large and low dose group of ShuGanAnShen. The two drugs could also apparently decrease sleeping incubation period and prolong sleep duration. Estazolam and large dose group of ShuGanAnShen could increase the rate of falling asleep and there was no significant difference between the two groups. No effect of biochemical indexes and toxicity of liver of rats were discovered during the treatment by prescription of ShuGanAnShen.
Conclusions:Prescription of ShuGanAnShen could decrease the frequency of rats’ autonomic activities and period of falling asleep. It could also prolong sleeping time and increase the rate of falling asleep of rats. The prescription had an obviously effect on sleeping and no effect on liver and biochemical indexes .This prescription had effective treatments on insomnia.
引文
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[3] Danie1 J,Buysse MD.Sleep disorders and psychiatry (Review of psyehiatry). Vol.24.Washington DC:American Dsychiatric Association,2005:185.
[4] Neil S. The physio1ogy of sleep and the impact of ageing.Europ Urol,2005,3(6):1723.
[5]王伟治.神经病学[M].人民卫生出版社.2005,第五版:257.
[6]杜冠华.实验药理学[M].中国协和大学出版社.2004,第五版:32-33.
[7]徐淑云,卞如濂,陈修.药理实验方法学[M].北京:人民卫生出版社,1982:465,479.
[8]吴江,贾建平,崔丽英;神经病学[M].北京:人民卫生出版社,2005(8):380.
[9]赵乐章;中缝背核5-经色胺能神经元在睡眠调节中的作用研究[J].中国应用生理学杂志,2003,19(2):175-78.
[10] Galindo-Charles Luis,Herandez-Lopez S,Galarraga E,et al.Serotoninergic Dorsal raphe neurons possess functional postsynaptic nicotinic acetylcholine raceptors.Synapse,2008,62(8):601-615.
[11] Landolt HP,Wehrle R.Antagonism of serotonergic 5-HT2A/2C receptors:mutual improvement of sleep,cognition and mood? European Joumal of Neuroscience; 2009,29(9):1795-1809.
[12] Lin Y,Quartermain D,Dunn Adrian J,et al.Possible dopaminergic stimulation of locus coeruleusαl-adrenoceptors involved in behavioral activation.Synapse,2008,62(7):516-523.
[13] Verret L,Fort P,Gervasoni D,et al;Localization of the neurons active during paradoxieal(REM)sleep and projecting to the locus coeruleus noradrenergic neurons in the rat·The Joumal of Comparative Neurology,2006,495(5):573-586.
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