犊牛口腔黏膜上皮细胞cDNA文库的构建及其水泡性口炎病毒受体的筛选
|
摘要
水泡性口炎病毒(Vesicular Stomatitis Virus, VSV)是引起多种动物水泡性口炎的一种重要人兽共患病病原,属于弹状病毒科的成员。VSV对马、牛、猪特别易感,羊、山羊、多种野生动物和人均有易感性。水泡性口炎与口蹄疫、猪水泡病、猪水泡疹的临床症状十分相似,以口腔黏膜、舌、唇、乳头和蹄冠部上皮出现水泡为主要特征。VSV对不同动物致病性的共同特征为具有嗜上皮性,根据这一特性开展该病毒的致病机制研究,对于水泡性口炎疾病的防治意义重大。
本研究以犊牛口腔黏膜上皮为材料,分离培养犊牛原代口腔黏膜上皮细胞,经形态学观察、细胞贴壁率和生长曲线计算、流式细胞仪检测等系统鉴定,确定已成功建立了一种原代犊牛口腔黏膜上皮细胞培养方法,并获得高纯度的原代上皮细胞,为研究具有嗜上皮性病毒的侵入机制和细胞cDNA文库构建提供了良好的实验体系。将VSV接种于犊牛口腔黏膜上皮细胞,应用超薄切片技术进行病毒的形态发生和细胞超微结构变化特征的观察,通过接毒细胞出现病变和电镜观察到弹状病毒样粒子,证实VSV可感染原代培养的犊牛口腔黏膜上皮细胞,并能繁殖扩增病毒。通过超微结构观察,初步明确VSV对该细胞的侵袭过程,且病毒通过细胞膜和胞浆内的空泡膜上出芽增殖后获得囊膜而成熟。将原代细胞大量培养增殖后,提取细胞的总RNA后分离其mRNA,利用噬菌体表面展示技术,构建了对VSV具有高反应性的CPMBCs高质量cDNA表达文库。未扩增文库的滴度为1.3×107 pfu/mL,文库重组率为95.8%,扩增后文库滴度为2.4×1010 pfu/mL。为高通量地从库中筛选出与VSV相互作用的噬菌体重组子,即寻找该病毒相互作用受体蛋白奠定了坚实的基础。用纯化的VSV与构建的T7噬菌体展示文库进行文库筛选,获得一个VSV可能的受体蛋白基因功能区片段。该基因片段长度为801bp,含有一大小为375bp的开放阅读框,编码124个氨基酸残基,将其命名为CPMBCsCP1。测序结果经登陆NCBI BLAST核酸同源性检索为与人类的剪接因子3b的同源性较高。DNAStar软件和ScanProsite分析结果表明是CPMBCsCP1蛋白亲水性较好,具有免疫原性和良好的抗原性,其生物学活性有可能在细胞信号传导通路中接受多种信号的调控。该项研究不仅为VSV由其受体介导的分子致病机制研究奠定坚实的基础,而且将为我国针对VSV引发疾病的防治研究提供参考依据。
Vesicular stomatitis(VS) is an acute, febrile and highly contagious infectious disease in cattle and in human which caused by vesicular stomatitis virus(VSV). The disease is characterized by vesic in the epithelium of oral mucosa, tongue, lip, papillae and coronet. The disease first occurred in the middle of United States and North America in 1821, then spreading to some countries and areas of South America, Africa, Europe and Asia. Since 1995, outbreaks of VSV had been reported in the western of United States one after another. VSV infection is frequently occurred in cattle, horses, pigs, deer and so on, but human could be infected occasionally. The mainly clinical sign of VSV infection is acute fever which is similar to Flu and Aden fever. In the summer of 2002, outbreaks of VSV were reported in Jilin province and Altay area in Xinjiang province of China. Although the infection spectrum of VSV is broad, the tropism epithelium is a common characteristics for different infected animals. However, up to now, the mechanism of tropism epithelial of VSV is not clear.
In this study, we first cultured calf primary oral mucosal epithelial cells in vitro. The result indicated that we had successfully established a high-grade cultural method of calf primary oral mucosal epithelial cells (CPMBCs) by the observation of morphology, optical microscopy, ultrastructure using scanning electron microscopy and transmission electron microscopy, the identification of cytokeratin by immunohistochemistry and the examination of flow cytometry. This would establish foundation for subsequent research. We observed the morphogenesis of VSV and the changed characteristic of cellular ultrastructure using ultrathin section by collecting cells in different time after infection. The result indicated that the CPMBCs appeared cytopathic effect(CPE) after infection of VSV. We observed virions like bullet by negative staining. The result confirmed that VSV could infect primary CPMBCs. We had initially identified the process of VSV infecting CPMBCs by the observation of cellular ultrastructure. We considered that the maturity of VSV was by pullulation proliferation in cellular membrane and cytoplasm.
The CPMBCs produced typical CPE after infection of VSV, so we supposed that the surface of CPMBCs should exist receptors of VSV. In order to more overall sieve the receptors of VSV, we first established T7 phage display library of CPMBCs in the research. The data showed that the library contained 1.3×107 clones, and approximately 95.8% of the library were recombinant. The titer of the amplied library was 2.4×1010 pfu/mL. The cDNA fragments longer than 300bp in length were 93% by using the PCR identification. The VSV virions after purification by sucrose gradient centrifugation were used to sieve receptors. We coated 96 ELISA plate with purificated virions, then added amplified library to carry out sieving receptors. We carried out five series sieves. At last we spread on the plate using the supernatant of the last serie. We picked out negative colonies, then extracted genome DNA from them. The cDNA inserts in these plaques were amplified by PCR using the primers. The phage DNA were sequenced , and the nucleotides were compared with the Genbank database by BLAST searches. Amino acid sequences were analyzed through ExPASy Proteomics Server and DNAStar software. About 100 clones were randomly picked from individual plaques, and their DNA sequences were amplified by PCR and analysed on agarose gel to determine the size of the inserts. The result of PCR analysis showed that 34% of the phage clones had an insert size of 400bp or longer. The sequence showed that the insert was 801bp in length and contained a 375bp open reading frame(ORF), which was predicted to encode 124 amino acids. The protein was designated CPMBCsCP1 and high homology appears between CPMBCsCP1 and the human splicing factor of 3b. The analytic result of CPMBCsCP1 protein had the characteristic of hydrophilicity, immunogenicity and good antigenicity, and biologic activity of the protein might be regulation by a variety of signals in the transmission pathway of cellular signals.
This study will not only establish a foundation for the study of VSV infectious mechanism mediated by receptors, but also provide certain theory basis for the research of prevention and cure of VSV infection in our country.
本研究以犊牛口腔黏膜上皮为材料,分离培养犊牛原代口腔黏膜上皮细胞,经形态学观察、细胞贴壁率和生长曲线计算、流式细胞仪检测等系统鉴定,确定已成功建立了一种原代犊牛口腔黏膜上皮细胞培养方法,并获得高纯度的原代上皮细胞,为研究具有嗜上皮性病毒的侵入机制和细胞cDNA文库构建提供了良好的实验体系。将VSV接种于犊牛口腔黏膜上皮细胞,应用超薄切片技术进行病毒的形态发生和细胞超微结构变化特征的观察,通过接毒细胞出现病变和电镜观察到弹状病毒样粒子,证实VSV可感染原代培养的犊牛口腔黏膜上皮细胞,并能繁殖扩增病毒。通过超微结构观察,初步明确VSV对该细胞的侵袭过程,且病毒通过细胞膜和胞浆内的空泡膜上出芽增殖后获得囊膜而成熟。将原代细胞大量培养增殖后,提取细胞的总RNA后分离其mRNA,利用噬菌体表面展示技术,构建了对VSV具有高反应性的CPMBCs高质量cDNA表达文库。未扩增文库的滴度为1.3×107 pfu/mL,文库重组率为95.8%,扩增后文库滴度为2.4×1010 pfu/mL。为高通量地从库中筛选出与VSV相互作用的噬菌体重组子,即寻找该病毒相互作用受体蛋白奠定了坚实的基础。用纯化的VSV与构建的T7噬菌体展示文库进行文库筛选,获得一个VSV可能的受体蛋白基因功能区片段。该基因片段长度为801bp,含有一大小为375bp的开放阅读框,编码124个氨基酸残基,将其命名为CPMBCsCP1。测序结果经登陆NCBI BLAST核酸同源性检索为与人类的剪接因子3b的同源性较高。DNAStar软件和ScanProsite分析结果表明是CPMBCsCP1蛋白亲水性较好,具有免疫原性和良好的抗原性,其生物学活性有可能在细胞信号传导通路中接受多种信号的调控。该项研究不仅为VSV由其受体介导的分子致病机制研究奠定坚实的基础,而且将为我国针对VSV引发疾病的防治研究提供参考依据。
Vesicular stomatitis(VS) is an acute, febrile and highly contagious infectious disease in cattle and in human which caused by vesicular stomatitis virus(VSV). The disease is characterized by vesic in the epithelium of oral mucosa, tongue, lip, papillae and coronet. The disease first occurred in the middle of United States and North America in 1821, then spreading to some countries and areas of South America, Africa, Europe and Asia. Since 1995, outbreaks of VSV had been reported in the western of United States one after another. VSV infection is frequently occurred in cattle, horses, pigs, deer and so on, but human could be infected occasionally. The mainly clinical sign of VSV infection is acute fever which is similar to Flu and Aden fever. In the summer of 2002, outbreaks of VSV were reported in Jilin province and Altay area in Xinjiang province of China. Although the infection spectrum of VSV is broad, the tropism epithelium is a common characteristics for different infected animals. However, up to now, the mechanism of tropism epithelial of VSV is not clear.
In this study, we first cultured calf primary oral mucosal epithelial cells in vitro. The result indicated that we had successfully established a high-grade cultural method of calf primary oral mucosal epithelial cells (CPMBCs) by the observation of morphology, optical microscopy, ultrastructure using scanning electron microscopy and transmission electron microscopy, the identification of cytokeratin by immunohistochemistry and the examination of flow cytometry. This would establish foundation for subsequent research. We observed the morphogenesis of VSV and the changed characteristic of cellular ultrastructure using ultrathin section by collecting cells in different time after infection. The result indicated that the CPMBCs appeared cytopathic effect(CPE) after infection of VSV. We observed virions like bullet by negative staining. The result confirmed that VSV could infect primary CPMBCs. We had initially identified the process of VSV infecting CPMBCs by the observation of cellular ultrastructure. We considered that the maturity of VSV was by pullulation proliferation in cellular membrane and cytoplasm.
The CPMBCs produced typical CPE after infection of VSV, so we supposed that the surface of CPMBCs should exist receptors of VSV. In order to more overall sieve the receptors of VSV, we first established T7 phage display library of CPMBCs in the research. The data showed that the library contained 1.3×107 clones, and approximately 95.8% of the library were recombinant. The titer of the amplied library was 2.4×1010 pfu/mL. The cDNA fragments longer than 300bp in length were 93% by using the PCR identification. The VSV virions after purification by sucrose gradient centrifugation were used to sieve receptors. We coated 96 ELISA plate with purificated virions, then added amplified library to carry out sieving receptors. We carried out five series sieves. At last we spread on the plate using the supernatant of the last serie. We picked out negative colonies, then extracted genome DNA from them. The cDNA inserts in these plaques were amplified by PCR using the primers. The phage DNA were sequenced , and the nucleotides were compared with the Genbank database by BLAST searches. Amino acid sequences were analyzed through ExPASy Proteomics Server and DNAStar software. About 100 clones were randomly picked from individual plaques, and their DNA sequences were amplified by PCR and analysed on agarose gel to determine the size of the inserts. The result of PCR analysis showed that 34% of the phage clones had an insert size of 400bp or longer. The sequence showed that the insert was 801bp in length and contained a 375bp open reading frame(ORF), which was predicted to encode 124 amino acids. The protein was designated CPMBCsCP1 and high homology appears between CPMBCsCP1 and the human splicing factor of 3b. The analytic result of CPMBCsCP1 protein had the characteristic of hydrophilicity, immunogenicity and good antigenicity, and biologic activity of the protein might be regulation by a variety of signals in the transmission pathway of cellular signals.
This study will not only establish a foundation for the study of VSV infectious mechanism mediated by receptors, but also provide certain theory basis for the research of prevention and cure of VSV infection in our country.
引文
[1]金宁一,胡仲明,冯书章.新编人兽共患病学[M].北京:科学出版社, 2007.09.
[2]于大海,崔砚林.中国出入境动物检疫规范[M].北京:中国农业出版社, 1997.
[3] Banerjee.A K. The transcription complex of vescular stomtitis virus. Cell.1987,48(3):363~364
[4] Rose J K.Schubert M.Genomes and their products.In Robert R , Wagner ed ,Rhabdovirues.New York:Plenum prees,1987.129-166
[5]殷震,刘景华.动物病毒学(第二版) [M].北京:科学出版社, 1997.11.
[6] Bourhy H,Molecular diversity of the lyssavirus genus,Virology,1993,194:70~81
[7] Richardsor JC,Peluso RW, Inhibition of VSV genome RNA replication but not tuanscription by monoclonal antibodies Specific for the viral P protein,Virology 1996 Feb 1;216(1):26~34
[8] Tordo N,Bourby H,Sather S, etal, Structure pression in bacular virus of the mookola virus glycoprotein, virology, 1993,184: 59~69
[9] Michael S N ,DM Hunt1Identificztion of an amino acid change that affects N protein function in resicular stomatitis virus [J]. Journal of General Virology,1994,75:3591– 3595
[10]Pierre Boudinot ,Sabine Riffault ,Samia Salhi ,et al1VesicularStomatitis Virus and pseudorabies virus induce a vigl/ cig5homdogue in mouse dendritic cells via different pathways [J ]1Journal of General Virology,2000,81:2675– 2682
[11]Josedel L,Barrera,Geoffrey J. Persistence of vesicular stomatitis virus NewJersey RNA in convalescent Hamsters [J ]. Virology,1996,219:453– 464
[12]Charlie D, Structrue of rhabdoviruses[J]. Virology,1999 (2):239– 260
[13]Das T,Gupta A K,Sims P W,et al. Role od cellular case in kinaseⅡin the function of the phosphoprotein(P) subunit of RNA polymerase of vesicular stomatitis virus[J]. The journal of biological chemistry,1995,27:4100– 4107.
[14]Von Kobbe ,et al1Molecular Biology of VSV[J ]1Molecular Cell12000 (6) :12431
[15]Grigera P R,Garcia - Briones M,Periolo O,et al1Immunogencity of an aphthovirus chimera of the glycoprotein of vesicular stomatitis virus[J ]1Journal of Virology ,1996 ,70(12) :8492– 85011
[16]Yang J,Hooper DC,Wunner WH et.al , The specificity of rabies virus RNA encapsidation by nucleoprotein Virology 1998,242;107~117
[17]Yoshiharu M,Hideki T,Kensuke D ,et al1Characterization of pseudotype VSV possessing HCV Envelpe proteins [J ]1Virology ,2001 ,286 :263– 2751
[18]Terstegen L,Gatsios P,Ludwig S,et.al The VSV M protein inhibits glycoprotein 130—dependentSTAT activation, J Immumol 2001 Nov I.167(9):5209~5216
[19]Michel lambed,Alain douart,Eliane joris,et al1Characterization of the innune response of cattle against non - cytopathic and cytopathic biotypes of bovine viral diarrhoea virus [J ]1Journal of General Virology,1997,78:1041–10471
[20]Schlegel R,Tralka TS,Willingham MC,et al. Inhibition of VSV binding and infectivity by phosphatidylserine is phosphatidylserine a VSV- binding site[J]. Cell. 1983,32:639–646.
[21]Schlegel R,Wade M. Neutralized vesicular stomatitis virus binds to host cells by a different "receptor" [J]. Biochem Biophys Res Commun. 1983, 114(2):774-778.
[22]Schlegel R,Willingham MC,Pastan IH. Saturable binding sites for vesicular stomatitis virus on the surface of Vero cells [J]. J Virol. 1982,43(3):871-875.
[23]David A,Coil,and A. Dusty Miller. Phosphatidylserine is not the cell surface receptor for vesicular stomatitis virus[J]. J. Virol. 2004,48(20):10920–10926
[24]Carneiro FA, Bianconi ML, Weissmuller G,et al. Membrane recognition by vesicular stomatitisvirus involves enthalpy-driven protein-lipid interactions[J]. J Virol. 2006,76(8):3756- 3764.
[25] Carneiro FA,Lapido-Loureiro PA,Cordo SM,et al. Probing the interaction between vesicular stomatitis virus and phosphatidylserine[J]. Eur Biophys J. 2006,35(2):145-154.
[26]Letchworth GJ,Rodriguez L L,Barrera J D C1 Vesicular Stomatitis[J ] The Veterinary Journal,1999,15:239 -260
[27]Angela Horn Montel,Grace Hommel- Berrey,Zacharie Brahmi1 Fas- Mediated cytotoxicity induces degradation of Vesicular Stomatitis Virus RNA transcripts and reduces virual titer [ J ] Molecular Immunology,1997,34 ( 15) 1055-10661
[28]Jack D K,Manfred S,Robert A L1 Interverning sequence between the leader region and the nucleocapsid gene of vesicular stomatitis virus RNA transcripts and reduces virual titer [J]. Journal of Virology,1980(5):789~794
[29]梁凤霞,丁明孝,翟中和水泡性口炎病毒的释放的方向性及其细胞骨架的关系[J].病毒学报,1993,9(2):167-170
[30]AhnnedM,Mckenzie MO,Puckettos,et.al, Ability of the Matrix protein of VSV to suppress beta interferon gene expression is genetically correlated with the inhibiton of host RNA and protein Syrthesis,J virol,2003 Apr;77(8):46~57
[31]Flanagan EB,Zamparo JM,Ball LA, et.al, Rearrangemont of the genes of VSV eliminates clinical disease in the natural host. J virol 2001 Jul,75(13):6107~6114
[32]Miller MA,lavine CL,Klas SD:Links Recombinant replication restricted vsv as an expression vector for murine cytokines,protein expr purify 2004.Jom 33(1):92~103
[33]Gupta AK,Banerjee AK, Expression and purification of VSV N-P complex from Es.col,J virol 1997 Jum 71(6):4264~4271
[34] Jayakar HR,whitt MA . Identification of two additional translation products from the matrix(M) gene than contribute to VSV cytopathology,J virol 2002,Aug;76(16):8011~8018
[35] Gaudier M,GaudinY,Cleavage of VSV M Protein prevents self-association and leads to crystallization[J]. Virology. 2001, 288(2):308~314
[36] Flangan EB,schoeb TR,Wertz Gw,VSV with rearranged genomes have altered invasiveness and neuropathogenesis in mice,J virol. 2003. 77(10):5740~5748
[37]Das T.pattnaik AK,Takacs AM et.al, Basic amino acid residues at the C-terminal eleven amino acid region of the P are required for transcription but not for replication of VSV genome RNA,Virology. 1997:238(1):103~114
[38]Whela SP,wertz GW,Regulation of RNA synthesis by the genomic termini of VSV,J Viro. 1999;73(1):297~306
[39]Mathieu ME,Grigera PR,Helenius A, et.al Folding,unfolding and refolding of the VSV glycoprotein,Biochemistry 1996. 35(13):4084~4093
[40]Da poian AT,Gomes AM.Oliveira RJ et.al,Migration of VSV glycoprotein to the nucleus of infected cells. Proc Nat I Acad Sci USA 1996 Aug 6;93(16):8268~8273
[41] Jayakar HR,MurtiKG,whitt MA, Mutations in the PPPY motif of vsv G Protein reduce virus budding by inhibiting a late step in virion release. J viral 2000 Nov;74(21):9818~9827
[42]Lyles DS,Mckenzie MO. Activity of VSV M protein mutants in cell rounding is correlated with the ability to inhibit host gene expression. Virology 1997 Mar 3;229(1):77~89
[43]Mathur M,Baner jee AK Novel binding of GTP to the P of VSV,Gene expr 2002;10(4):193~200
[44]Tordo N.Molecular epidemiology of lyssaviruses Focus on the glycoprotein and pseudogenes J vet . Res 1993,60:315~323
[45]靳喜朋,褚秀玲,霍惠玲.水泡性口炎的发病机制和临床诊治[J].张家口农专学报,2003,19 (2):8.
[46]黄运生.间接ELISA诊断水泡性口炎[J].中国兽医杂志,1989, 15 (4): 47.
[47]Ahmad S,Bassiri M,Banerjee AK,Yilma T. Immunological characterization of the VSV nucleocapsid (N) protein expressed by recombinant baculovirus in Spodoptera exigua larva:use in differential diagnosis between vaccinated and infected animals.
[48]Afshar A,Shakarchi N H,Dulac G C.Development of a competitive enzyme linked immunosorbent assay for detection of bovine,equine,ovine and porcine antibodies to vesicular stomatitis virus.J Clin Microbiol,1993,31:1860～1865
[49]Vernon S D , Webb P A , Recent vesicular stomatitis virus infection detected by immunoglobulin M antibody capture enzyme-linked immunosorbent assay.J Clin Microbiol,1985,22(4):582～586
[50]花群义,金宁一,徐自忠.水泡性口炎病毒核蛋白基因的表达及初步应用[J].生物工程学报,2004,20 (1):130.
[51]Rodriguez L L,Letchworth G J,Spiropoulou C F,Rapid detection ofvesicular stomatitis virus New Jerseyserotype in clinical samples by usingPCR[J ] . Journal of clinical microbiology,1993,(8):2016 - 2020.
[52]Hofner M C,Carpenter W C,Ferris N P A hemi - nested PCR assay for the detection and indentification of vesicular stomatitis virus nucleic acid. Journal of Virological Methods,1994,50:11-20.
[53]Ignacionunez,Blanco E,HernandezTA RT- PCR assay for the differential diagnosis of vesicular viral disease of swine[J ] . Journal of Virological Methods, 1998,72:227-235.
[54]Rasmussen TB,Uttenthal A,Fernández J,Storgaard T. Quantitative multiplex assay for simultaneous detection and identification of Indiana and New Jersey serotypes of vesicular stomatitis virus.J Clin Microbiol. 2005 Jan;43(1):356-362.
[55]花群义等;水泡性口炎检测技术体系的建立2005.12.24,科技成果
[56]杨桂梅,徐自忠. VSV的RT-PCR快速检测方法的建立[J].动物科学与动物医学,2003,20 (2):23.
[57]陈茹.水泡性口炎病毒血清型特异LUX实时荧光RT-PCR检测方法的建立.畜牧兽医学报. 2008, 39(4):522-528
[58]杨桂梅,徐自忠.二重RT-PCR同时检测VSV与BVDV核酸[J].中国预防兽医学报,2003,25 (4):291.
[59] Nunez JI,Blanco E,Hernandez T,et al. A RT-PCR assay for the differential diagnosis of vesicular viral diseases ofs wine [J]. J Virol Methods,1998,72 (2): 227.
[60] Reid SM,Ferris NP, Hutchings GH, et al. Primary diagnosisof foot-and-mouth disease by reverse transcription polymerase chain reaction [J]. J Virolo Methods, 2000,89 (1-2):167.
[61]徐自忠等,水泡性口炎、口蹄疫、猪水泡病和猪瘟检测技术,科技成果. 2005.12.11,
[62] Krumbholz A,Wurm R,Scheck O,et al. Detection of porcine teschoviruses and enteroviruses by LightCycler realtime PCR [J]. J Virol Methods, 2003, 113 (1):51.
[63] Reid SM,Ferris NP,Hutchings GH,et al. Evaluation of realtime reverse transcription polymerase chain reaction assays for the detection of swine vesicular disease virus[J]. J Virol Methods, 2004, 116 (2): 169.
[64] Afshar A, Dulac GC, Wright PF, et al. Application of indirect ELISA for detection of bovine antibodies against vesicular stomatitis viruses [J]. J Vet Diagn Invest, 1993, 5(1): 26.
[65]花群义等.水泡性口炎病毒核蛋白基因的克隆和序列分析.中国生物制品学杂志: 2004, 17 (1): 4.
[66]Katsunori Shinozaki,Oliver Ebert,Savio LC Woo,et al. Treatment of multifocal colorectal carcinoma metastatic to the liver of immune competent and syngeneic rats by hepatic artery infusion of oncolytic vesicular stomatitis virus.Cancer,2005,114(6):659～664
[67]David FS,Brian L,Shane K,et al. Exploiting tumor specific defects in the interferon pathway with apreviously unknown oncolytic virus.N at Med,2000,6(7):821～832
[68]Lee SB,Bablanian R,Esteban M,et al.Regulated expression of the Interferon induced protein kinase p68(PKR) by vaccinia virus recombinants inhibits the replication of vesicular stomatitis virus but not that of poliovirus.Interferon Cytokine Res,1996,16(12):1073～1084
[69]Jin HK,Takada A,Kon Y,et al.Identification of the murine Mx-gene:interferon induced expression of the Mx-protein from the feral mouse gene confers resistance to vesicular stomatitis virus.Virol,1999,73(6):4925～4932
[70]Steinhoff U,Muller U,Schertler A,et al. A ntiviral protection by vesicular stomatitis virusspecific antibodies in alpha/beta interferon receptor deficient mice.Virol,1995,69(4):2153～2163
[71] Balachandran S,Barber GN.Vesicular stomatitis virus(VSV) therapy of tumos. IUBMB Life,2000,50(2):135～138
[72]Siddharth B,Mercedes P,Glen N B,et al. Oncolytic activity of vesicular stomatitis virus is effective against tumors exhibiting Aberrant p53,Ras,or Myc function and involves the induction of apoptosis.Virol,2001,75(7): 3473～3479
[73] Ezelle H J,Markovic D,Barber G N,et al. Generation of Hepatitis C Virus-Like Particles by Use of a Recombinant Vesicular Stomatitis Virus Vector. Virol,2002,76(5):12325～12334
[74]Haglund K,Forman J,Kraèusslich H G,et al. Expression of Human Immunodeficiency Virus Type 1 Gag Protein Precursor and Envelope Proteins from a Vesicular Stomatitis Virus Recombinant:High-Level Production of Virus-like Particles Containing HIV Envelope. Virology,2000,26(8):112～121
[75]Kapadiaa S U,Rosea J K,Lamirande E.et al. Long-term protection from SARS coronavirus infection conferred by a single immunization with an attenuated VSV based vaccine.Virology,2005,340(3):174～182
[76]Publicover J,Ramsburg E ,Rose J K,et al. Asingle-cycle vaccine vector Based on vecicular etomamtitis virus can induce immune responses comparable to those generated by a replication copetent vector.Virol,2005,79(3):13231～13238
[77]Kretzschmar E,Buonocore L,Schnell M J,et al.High-Efficiency Incorporation of Functional Influenza Virus Glycoproteins into Recombinant Vesicular Stomatitis Viruses.Virol,1997,71(3):5982～5989
[78]Rajcani J. Molecular mechanisms of virus spread and virion components as tools of virulence [J]. Acta Microbiol Immunol Hung,2003, 50 (4): 407-443
[79]Norkin LC. Virus receptor: implications for pathogenesis and design of antiviral agents[J]. Clin Micro Rev, 1995,8(2):293
[80]Monaghan P, Gold S, Simpson J, et al. Theαvβ6integrin receptor for foot-and-mouth disease virus is expressed constitutively on the epithelial cells targeted in cattle[J]. J Gen Virol, 2005,86:2769
[81]Mendelsohn CL et al . Cell ,1989 ,56(10) :855—865
[82]Cattaneo R. Four viruses, two bacteria, and one receptor: membrane cofactor protein (CD46) as pathogens' magnet [J]. J Virol, 2004, 78(9): 4385-4388.
[83]Maurer K, Krey T, Moennig V, et al. CD46 is a cellular receptor for bovine viral diarrhea virus [J]. J Virol, 2004, 78 (4): 1792-1799.
[84]Masciopinto F,Campagnoli S,Abrignani S,et al. The sma llextracellular loop of CD81 is necessary for optimal surface expression of the large loop,a putative HCV receptor [J]. Virus Research,2001,80 (1-2):1-10.
[85]Sieczkarski SB,Whittaker GR. Virla entry[J]. Curr Top MicrobiolImmunol,2004,285:1
[86]Schols D. HIV co- receptor inhibitors as novel class of anti- HIV drugs[J]. Antiviral Res, 2006,71(3):216
[87]Jackson T,King A M,Stuart D I,et al. Structure and receptor binding. Virus Res, 2003,91 (1):33-46.
[88]Chen B, Vogan EM, Gong HY, et al. Determining the structure of an unliganded and fully glycosylated SIV gp120 envelope glycoprotein[J].Structure,2005,13(2):197
[89]Jackson T,King AM,Stuart D,et al. Structure and receptor binding [J]. Virus Res,2003,91:33
[90]Urban S, Breiner KM, Fehler F, et al. Avian hepatitis B virus infection is initiated by the interaction of a distinct pre-S subdomain with the cellular receptor gp180. J Virol. 1998, 72(10): 8089-8097.
[91]Schnell MJ, Buonocore L, Whitt MA, et al. The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. J Virol. 1996; 70(4): 2318-2323.
[92]Thomssen R, Bonk S, Propfe C, et al. Association of hepatitis C virus in human sera withβ-lipoprotein [J]. Med Microbiol Immunol, 1992, 181 (5): 293-300.
[93]Monazahian M, B?hme I, Bonk S, et al. Low density lipoprotein receptor as a candidate receptor for hepatitis C virus [J]. J Med Virol,1999, 57 (3):223-229.
[94]Agnello V,Abel G,Elfahal M, et al. Hepatitis C virus and other Flaviviridae viruses enter cells via low density lipoprotein receptor [J]. Proc Nat Acad Sci USA,1999,96 (22):12766-12771.
[95]Irina NG,Michael S,Robert S,et al .β3 integrin mediate the cellular entry of hantavirus that cause respiratory failure. Proc Natl Acad Sci USA,1998,95:7074-7079.
[96]Qing KY,Cathryn M,Jonathan H ,et al . Human fibroblast growth factor receptoel is a co2receptor for infection by adeno2associated virus 2. Nature medicine,1999,5:71-77.
[97]Deng HK,Liu Rong ,Wilfried E ,et al . Identification of a major co-receptor for primary isolates of HIV21. Nature,1996,381:6612666.
[98]Dalziel R G, Hopkins J, Watt N J, et al. Identification of a putative cellular receptor for the lentivirus visna virus [J]. J Gen Virol,1991,72 (Pt8):1905-1911.
[99]Tallet, B. et al. Expression,urification and biological properties of the carboxyl half part of the HTLV-I surface envelope glycoprotein [J]. J. of Chromatography B. 2000,737:85-95.
[100]Angus GD,Peter CLB,Paul RC,et al . The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature,1984,312:763-766.
[101]Maurer K,Krey T,Moennig V,et al. CD46 is a cellular receptor for bovine viral diarrhea virus [J]. J Virol,2004,78 (4):1792-1799.
[102]Dorig R E,Marcil A,Chopra A,et al. The human CD46 molecule is a receptor for measles virus (Edmonston strain) [J]. Cell,1993,75 (2):295–305.
[103]Yeager C L,Ashmun R A,Williams RK,et al. Human aminopeptidase N is a receptor for human coronavirus 229 E [J]. Nature,1992;357:420-422.
[104]Naniche D,Varior-Krishnan G,Cervoni F,et al. Human membrane cofactor protein (CD46) acts as a cellular receptor for measles virus [J]. J Virol,1993,67 (10):6025–6032.
[105]Hanham C A,Zhao F S,Tignor G H. Evidence from the anti-idiotypic network that the acetylcholine receptor is a rabies virus receptor [J]. J Virol,1993;67(1):530-542.
[106]Wang K S,Schmaljohn A L,Kuhn R J,et al. Anti-idiotypic antibodies as probes for the Sindbis virus receptor [J]. Virology,1991;181 (2);694-702.
[107]Haywood A M. .Virus receptors: binding, adhision strengthening and changes in viral structure [J]. Virol,1994,68(1):1-5.
[108]陆慧君,贺文琦,高丰,等.猪血凝性脑脊髓炎病毒PK细胞膜受体的鉴定[J].农业生物技术学报. 2008,16(1):20-23.
[109]Salas-Benito J S,Angel R M D.Identification of two surface proteins from C6/36 cells that bind dengue type4 virus[J]. J Virol,1997,71(10):7246-7252.
[110]尹崇,余健秀等.斜纹夜蛾多角体病毒包埋型受体的鉴定[J].中国病毒学. 2002, 17(1): 66-68.
[111]郭爱珍,陆承平.犬瘟热病毒细胞膜受体的鉴定[J].病毒学报,2000,16(2):155-157.
[112]Maisner A,Schneider-Schaulies J,Liszewski M K,et a1.Binding of measles virus to membrane cofactor protein(CD46):importance of disulfide bonds and N-glycans for the receptor function[J].J Virol,1994,68(10):6299-6304
[113]Gastka M Horvath J,Lentz TL.Rabies virus binding to the nicrotinic acetylcholine recepttor alpha subunit demonstrated by virus overly protein binding assay[J].J Gen Virol,1996,77(10):2437-2440
[114] Karger A,Mettenleiter T C.Identification of cell surface molecules that interact with pseudorabies virus[J]. J Virol. 1996,70(4):2138-2145
[115] Fields B N. Fundamental Virology 2nd ed. [M].New York: Raven Press,1990,87-95.
[116]Wu E,Trauger S A,Pache L,et al. Membrane cofactor protein is a receptor for adenoviruses associated with epidemic keratoc onjunctivitis [J]. J Virol,2004,78 (8):3897–3905.
[117] Haywood A M.. Virus receptors:binding,adhision strengthening,and changes in viral structure. J.Virol.,1994,68(1):1-5.
[118]Salas-Benito J S,Angel R M D.Identification of two surface proteins from C6/36 cells that bind dengue type4 virus[J]. J Virol,1997,71(10):7246-7252.
[119] Smith G P. Filamentous fusion phage:novel expression vectors that display cloned antigen on the virion surface [J]. Science. 1985,228:1315-1317.
[120]Paul P Sche,Kathleen M Mckenzie,et a1. Display cloning:fuctional identification of natural product receptors using cDNA-phage display[J]. Chemistry & Biology,1999,6:707-716.
[121]董倩,施双双,王业东,等. cDNA文库噬菌体展示法的建立及乙型肝炎病毒前S1蛋白结合蛋白筛检[J].解放军医学杂志. 2002, 4(27): 321-322.
[122]Hong S S,Karayan L,Tournier J,et al. Adenovirus type 5 fiber knob binds to MHC class Iα2 domain at the surface of human epithelial and B lymphoblastoid cells [J]. EMBO J,1997,16(9):2294-2306.
[123]Yu F, Christopher CB,Paul EK,et al. HIV-1 entry cofactor:functional cDNA cloning of a seven-transmembrane G protein-coupled receptor[J]. Science,1996, 272:872-877.
[124]Young JA,Bates P,Varmus HE.Isolation of a chicken gene that confers susceptibility to infection by subgroup A avian leukosis and sarcoma viruses[J] Virol. 1993,67(4):1811-1816.
[125]Greve JM Davis G, Meyer AM, et a1. The major human rhinovirus receptor is ICAM-1[J]. Cell,1989,56(5):839-847.
[126]Pileri P,Uematsu Y,Campagnoli S,et al .Binding of hepatitis C Virus to CD81. Science,1998,282 :338-341.
[127]Li WH,Moore MJ,Vasillieva N,et aL.Angiotension converting enzyme 2 is a functional receptor for SARS coronavirus[J].Nature,2003,426(6965):450-454.
[128]刘美德,赵彤言等. C6/36细胞上登革2型病毒受体的免疫共沉淀[J].中国病毒学. 2004,19(3):217-219.
[129]Ryu C J,Cho D Y,Gripon P,et al. An 80-kilodalton protein that binds to the Pre-S1 domain of hepatitis B virus [J]. J Virol,2000,74(1): 110-116.
[130]Fields S,Song O. A novalgenetic system to detect protein-protein interactions[J ]. Nature,1989,340:245-246.
[131]Li L Y,Liu X,Zhang P,et al. Cloning and functional identification of measles virus receptor on marmoset cells [J]. Chin Sci Bull,2002,47 (16):1217-1225.
[132]Aronheim A,Zandi E,Hennemann H,et al. Isolation of an AP-1 represso by a novel method for detecting protein-protein interactions[J]. Mol Cell Biol. 1997,17:3094-3102
[133]Johnsson N , V arshavsky A: Split ubiquitin as a sensor of protein interactions in vivo. Proc NatlA cad Sci USA 1994, 91: 10340-10344.
[134]Gaggar A,Shayakhmetov D M, Lieber A. CD46 is a cellular receptor for group B adenoviruses[J]. Nat Med,2003,9(11):1408-1412.
[135]Tio P H,Jong W W,Cardosa M J. Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1,2 and 3 [J]. Virology,2005.2 (1):25-36.
[136]Reyes-Del Valle J, Chavez-Salinas S, Medina F, et al. Heat shock protein 90 and heat shock protein 70 are components of dengue virus receptor complex in human cells[J]. J Virol,2005,79(8):4557-4567.
[137]Macbeath G,Schreiber SL. Printing proteins as microarrays for high-throughput function determination[J]. Science. 2000,289(5485):1673.
[138]王战会,靳刚.光学椭偏成像技术在生物分子研究中的应用[J].生物工程学报.2000,16(4):429-432.
[139] Jin G,Zhao ZY,Wang ZH et al. IEEE Conference ISIK2000 Workshop on Biomedical Information Engineering. Istanbul Turkey. 2000,137-140.
[140]Lofas S, et al. Bioanalysis with surface plasmon resenance,Sensor and Actuators,1991,B 5.:5-7
[141]Schreiber G. Kinetic studies of protein2protein interactions. Curr Opin Struct Biol ,2002 ,12(1) :41～47
[142] Schreiber G. Kinetic studies of protein2protein interactions. Curr Opin Struct Biol ,2002 ,12(1) :41～47
[143] Smith G P. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface [J]. Science, 1985, 228: 1315~1317.
[144] Dulbecco R, et al. US 1986, No. 4593002.
[145] Scott J K, Smith G P. Searching for peptide ligands with an epitope library [J].Science, 1990, 249: 386~390.
[146] Cwirla S E, Peters E A, Barrett R W, et al. Peptides on phage: A vast library of peptide for identifying ligands [J]. Proc Natl Acad Sci, USA, 1990, 87: 6378~6382.
[147] Devlin J J, Pangainban L C, Devlin P. Random peptide libraries: a source of specific protein binding molecules [J]. Science, 1990, 249: 404~406.
[148] Parmley J F, Smith G P, et al. Antibody-selectable filamentous fd phage vectors: affinity pacification of target genes [J]. Gene, 1988, 73: 305~318.
[149] Geysers H M, Rodda S J, Mason T J, et al. A priori delineation of a peptide which mimics a discontinuous antigenic determinant [J]. Immunol, 1986, 23: 709~715.
[150] McLafferty M A, Kent R B, L.adner R C, et al. M13 bacteriophage displaying disulfide-constrained microproteins [J]. Gene, 1993, 128: 29~36.
[151] Wang Y A, Yu X, Overman S, et al. The structure of a filamentous bacteriophage [J].J Mol Biol. 2006, 361(2): 209~215.
[152] Akita N, Maruta F, Sevmour L W, et al. Identification of oligo peptides binding toperitoneal tumors of gastric cancer [J]. Cancer Sci, 2006, 97 (10): 1075~1081.
[153] Sadanandam A, Varney M L, Kinarsky L, et al. Identification of Functional Cell Adhesion Molecules with a Potential Role in Metastasis by a Combination of in vivo Phage Display and in silico Analysis [J]. OMICS, 2007, Spring 11 (1): 41~57.
[154] Deroo S, Muller C P. Antigenic and immunogenic phage displayed mimotopes as substitute antigens: applications and limitations [J]. Combin Cheer Highthrouput Screen, 2001, 4: 75~110.
[155] Bartoli F, Nuzzo M, Urbanlli T, et al. DNA-based selection and screening of peptide ligands [J]. Nat. Biotechnol, 1998, 16: 1068~1073.
[156] Messmer B T, Benham C J, Thaler D S, et al. Sequential determination of ligands binding to discrete components in heterogeneous mixtures by interactive panning and blocking(IPAB) [J]. J Mol Biol, 2000, 296: 821~832.
[157]高鹏,黄秉仁.噬菌体展示文库及其应用[J].国外医学分子生物学分册, 2001, 23(6): 332-334.
[158]Benhar I. Biotechnological applications of phage and cell display [J]. Biotechnology Advances, 2001, 19 (1): 1~33.
[159] Benhar I, Azriel R, Nahary L, et al. Highly efficient selection of phageantibodies mediated by display antigen expression as LPP-OmpA′fusions on live bacteria [J]. J Mol Biol, 2000, 301 (4): 893~904.
[160] Ruud M T, de Wildt, Ian M Tomlinson, et al. Isolation of receptor-ligand pairs bycapture of long-lived multivalent interaction complexes [J]. Proc Natl Acad Sci USA, 2002, 99: 8530~8535.
[161]夏慧卿,邓洪宽,孙磊,崔国祯等,噬菌体展示文库筛选技术的研究进展,生命科学研究,2007 ,11 (1) : 50～53.
[162]Marco Antonio Arap. Phage display technology-Applications and innovations [J].Genetics and Molecular Biology, 2005, 28 (1): 1~9.
[163]赵慧斌,李晓军,武建国.噬菌体抗体库筛选技术[J].临床检验杂志, 2004, 22 (2) 46~148.
[164]Fukuda M N. Screening of Peptide-displaying phage libraries to identify short peptides mimicking carbohydrates [J]. Methods Enzymol, 2006, 416: 51~60.
[165]Aleksandr V, Pereboev 1, Naseem Ahmed, et al. Epitopes in the interacting regions of L-dystroglycan (PPxY motio and dystrophin (WW domain) [J]. Biochimica et Biophysica Acta, 2001, 1527: 54~60.
[166]Keresztessy Z, Csosz E, Harsfalvi J, et al. Phage display selection of efficient glutamine-donor substrate peptides for transglutaminase 2 [J]. Protein Sci, 2006, 15(11): 2466~2480.
[167]Koiwnen E, Arap W, Rajotte D. Identification of receptor ligands with phage display peptide libraries [J]. J Nuc Med, 1999, 40: 883~888.
[168]Awad A Osman, Holm Uhlig, Barbara Thamm, et al. Use of the phage display technique fordetection of epitopes recognized by polyclonal rebbit gliadin antibodies [J]. FEBS Letters, 1998, 433: 103~107.
[169]Ozawa M, Ohashi K, Onuma M. Identification and characterization of peptides binding to newcastle disease virus by phage display [J]. J Vet Med Sci, 2005, 67 (12):1237~1241.
[170]Mazzuccbelli L, BurritL B, Jesaitis A J, et al. Cell- specific peptide binding by human neutrophils [J]. Blood, 1999, 93 (5): 1738~1748.
[171]Kassner P D, Burg M A, Baird A, et al. Genetic selection of phage engineered for receptor- mediated gene transfer to mammalian cells [J]. Biochem Biophys Res Commun, 1999, 264 (3): 921~928.
[172]Pasqualini R, Ruoslahti E, et al. Organ targeting in vivo using phage display peptide libraries [J]. Nature, 1996, 380: 364~366.
[173]Rajotte D, Ruoslahti, et al. Membrane dipeptidase is the receptor for a lung-targeting peptide identified by in vivo phage display [J]. J Biol Chem, 1999, 274:11593~11598.
[174]Akita N, Maruta F, Sevmour L W, et al. Identification of oligo peptides binding to peritoneal tumors of gastric cancer [J]. Cancer Sci, 2006, 97 (10): 1075~1081.
[175]Sadanandam A, Varney M L, Kinarsky L, et al. Identification of Functional Cell Adhesion Molecules with a Potential Role in Metastasis by a Combination of in vivo Phage Display and in silico Analysis [J]. OMICS, 2007, Spring 11 (1): 41~57.
[176]Deroo S, Muller C P. Antigenic and immunogenic phage displayed mimotopes as substitute antigens: applications and limitations [J]. Combin Cheer Highthrouput Screen, 2001, 4: 75~110.
[177]Bartoli F, Nuzzo M, Urbanlli T, et al. DNA-based selection and screening of peptide ligands [J]. Nat. Biotechnol, 1998, 16: 1068~1073.
[178]Messmer B T, Benham C J, Thaler D S, et al. Sequential determination of ligands binding to discrete components in heterogeneous mixtures by interactive panning and blocking(IPAB) [J]. J Mol Biol, 2000, 296: 821~832.
[179]魏东芝,赖敏.噬菌体抗体库筛选技术[J].生命科学, 2000, 12 (3): 134~137.
[180]Benhar I. Biotechnological applications of phage and cell display [J]. Biotechnology Advances, 2001, 19 (1): 1~33.
[181] Benhar I, Azriel R, Nahary L, et al. Highly efficient selection of phageantibodies mediated by display antigen expression as LPP-OmpA′fusions on live bacteria [J]. J Mol Biol, 2000,301 (4): 893~904.
[182] Ruud M T, de Wildt, Ian M Tomlinson, et al. Isolation of receptor-ligand pairs by capture of long-lived multivalent interaction complexes [J]. Proc Natl Acad Sci USA,2002, 99: 8530~8535.
[183]刘志刚,俞炜源.新型噬菌体表面呈现载体的构建[J].生物技术通讯, 2002, 13 (1) : 50～53.
[184] Marco Antonio Arap. Phage display technology-Applications and innovations [J].Genetics and Molecular Biology, 2005, 28 (1): 1~9.
[185] Wind T, Stausbol G B, Kjaer S, et al. Retrieval of phage displayed scFv fragmentsusing direct bacterial elution [J]. J Immunol Methods, 1997, 209 (1): 75~83.
[186] Vatalit iA et al. Identification and characterization of peptides binding to newcastle disease virus by phage display.Cancer Res, 2000; 60 ( l6) : 4311
[187] Shane A et al. Cell-specific peptide binding by human neutrophils .Proc Natacad Sci USA , 1999; 96: 9497
[188] Daniel R et al. Genetic selection of phage engineered for receptor-mediated gene transfer to mammalian cells[J]. Biochem Biophys Res Commun, J Clin Invest, 1998; 102: 430
[189] MiChael S et al. Targeting in vivo using phage display peptide libraries J Biol Chem, 1997; 272(44) : 27943
[190]Parmley SF, Smith GP. dentification Identification of oligo peptides binding to peritoneal tumors of gastric cancer. Gene, 1988; 73: 305-318
[191]Kay BK. Antigenic and immunogenic phage displayed mimotopes as substitute antigens: applications and limitations Gene, 1993; 128: 59
[192] Cw irla SE. DNA-based selection and screening of peptide ligands Science. 1997; 274: l696
[193] Hoess RH. Sequential determination of ligands binding to discrete components in heterogeneous mixtures by iteractive panning and blocking. Biol, 1993; 3: 572
[194]Swimmer C et al. Phage Display of Ricin B Chain and its Single Binding Domains: System for Screening Galactose-Binding Mutants Proc N at l Acad Sci U SA , 1992; 89:
[195]SggioI et al. Highly efficient selection of phage antibodies mediated by display antigen expression as LPP-OmpA primer fusions on live bacteria.Gene, l995; 152: 35
[196]Choo Y et al. Isolation of receptor-ligand pairs by capture of longlived multivalentinteraction complexes Nature, 1994; 372: 642
[197]Chang TY. Transfusion, 2001; 41 (1) : 6
[198]周俊梅.噬菌体表面展示及其与疫苗研制有关的应用进展[J].国外医学.寄生虫病分册, 1998, 6, 241~246.
[199]Takagi T, Arisawa T, Yamamoto K, et al. Identification of ligands binding specifically to inflammatory intestinal mucosa using phage display [J]. Clin Exp Pharmacol Physiol, 2007, 34 (4): 286~289.
[200] Xu L, Jin B Q, Fan D M. Selection and identification of mimic epitopes for gastric cancer associated antigen MG7-Ag [J]. Cancer Ther, 2003, 2: 301-306.
[201] Romanov V I, Durand D B, Petrenko V A. Phage display selection of peptides that affect prostate carcinoma cells attachment and invasion [J]. Prostate, 2001, 47:239~251.
[202] Zhang Y, Chen J, Zhang Y et al. Panning and Identification of a Colon Tumor Binding Peptide from a Phage Display Peptide Library [J]. J Biomol Screen, 2007,[Epub ahead of print].
[203] Garamet ikoff K et al. Phage display technology-Applications and innovations Nucl Acids Res, 1994; 22; 5761
[204] Tanskanen J et al. Construction of a Multihybrid Display System: Flagellar Filaments Carrying Two Foreign Adhesive Peptides App l Environ M icrobio l, 2000; 66(9) : 4152
[205]沈霞芬主编,家畜组织学与胚胎学[M].中国农业出版社,第三版,2000.
[206] Wille JJ,Mansson-Rahemtulla B,Rahemtulla F.Characterization of human gingival eratinocytes culture in a serumfree medium[J].Arch Oral Biol,1990, 35(12):967-976.
[207]Oda D,Savard CE,Eng L,et al.Reconstituted human oral and esophageal mucosa in culture[J]. In Vitro Cell Dev Biol Anim,1998,34(1):46-52.
[208]Sugimura Y,Hata K,Torii S et al.Transplantation of cultured mucosal epithelium:an experimental study.J Craniomaxillofac Surg,1996,24 (6):352-359.
[209]Gibson P,Rosella O,Young G,et al.Serum free medium increases express- ion of markers of differentiation in human colonic crypt cells. Gut,1994,35(6):791-797.
[210]Reid C,Cloos J,Snow G,et al. A simple and reliable technique for culturing of human oral keratinocytes and fibroblast. Acta Otolaryngol, 1997,117:628-634.
[211]Ueda M,Hata K,Horie K,et al.The potential of oral mucosal cells for cultured epithelium:a preliminary report.Ann Plast Surg,1995,35 (5):498-504.
[212]Germain L,Rouabhia M,Guignard R,et al.Improvement of human keratinocyte isolation and culture using thermolysin.Burns,1993,19∶99.
[213]李宏卫,温玉明,王昌美,祁兵,等.人口腔黏膜上皮细胞的培养[J].南京医科大学学报,2003,23(4):379-381.
[214]Bergmann J E,Singer SJ.Immunoelectron microscopic studies of the intracellular transport of the membrane glycoprotein(G) of vesicular stomatitis virus in infected Chinese hamster ovary cells[J]. J Cell Biol,1983,97 (6):1777- 1787.
[215] Blumberg B M, Leppert M, Kolakofsky D. Interaction of Vesicular Stomatitis Virus leader RNA and nucleocapsid protein may control Vesicular Stomatitis Virus genome replication[J]. Cell,1981,23 (3):837– 845.
[216]王斌,李霞,高船舟.皱纹盘鲍一种球形病毒的感染及其发生[J].中国病毒学,1994,4(12):360-363.
[217] Ono K,Dubois-Dalcq M E, Schubert M et al.A mutated membrane protein of Vesicular Stomatitis Virus has an abnormal distribution within the infected cell and causes defective budding[J].J Viro1,1987,61(5):1332- 1341.
[218] Lisa D. Chong and John K. Rose . Membrane association of functional vesicular stomatitis virus matrix protein in vivo[J]. J. Virol.,1993;67:407-414.
[219] Brian D. Lichty,Heidi McBride,Stephen Hanson,et al. Matrix protein of Vesicular stomatitis virus harbours a cryptic mitochondrial-targeting motif[J]. J. Gen. Virol.,2006;87:3379-3384.
[220] Kalervo Metsikko and Kai SimonsThe budding mechanism of spikeless Vesicular Stomatitis virus particles[J].The EMBO Journal,1986,5(4):1913-1920.
[221] Jian Zhou and Gary W. Blissard. Display of Heterologous Proteins on gp64null Baculovirus Virions and Enhanced Budding Mediated by a Vesicular Stomatitis Virus G-Stem Construct[J]. J. Virol., 2008; 82: 1368-1377.
[222] Takacst A M, Banerjee A K. Inhibition of vesicular stomatitis virus in cells constitutively expressing an antisense RNA targeted against the virus RNA polymerase gene [J]. J Gen Virol.. 1997, 78(pt1): 125-129.
[223] Lambot M, Douart A, Joris E, et al. Characterization of the immune response of cattle against non-cytopathic and cytopathic biotypes of bovine viral diarrhoea virus [J]. J Gen Virol. 2006; 78 ( Pt 5):1041-1047.
[224] Schlegel R, Tralka TS, Willingham MC, et al. Inhibition of VSV binding and infectivity by phosphatidylserine: is phosphatidylserine a VSV- binding site[J]. Cell. 1983, 32:639–646.
[225] Schlegel R, Wade M. Neutralized vesicular stomatitis virus binds to host cells by a different "receptor" [J]. Biochem Biophys Res Commun. 1983, 114(2):774-778.
[226] Schlegel R, Willingham MC, Pastan IH. Saturable binding sites for vesicular stomatitis virus on the surface of Vero cells [J]. J Virol. 1982, 43(3): 871-875.
[227] David A, Coil, and A. Dusty Miller. Phosphatidylserine is not the cell surface receptor for vesicular stomatitis virus[J]. J. Virol. 2004, 48(20):10920–10926.
[228] Carneiro FA, Bianconi ML, Weissmuller G, et al. Membrane recognition by vesicular stomatitis virus involves enthalpy-driven protein-lipid interactions[J]. J Virol. 2006, 76(8): 3756- 3764.
[229] Carneiro FA, Lapido-Loureiro PA, Cordo SM, et al. Probing the interaction between vesicular stomatitis virus and phosphatidylserine[J]. Eur Biophys J. 2006, 35(2):145-154.
[2]于大海,崔砚林.中国出入境动物检疫规范[M].北京:中国农业出版社, 1997.
[3] Banerjee.A K. The transcription complex of vescular stomtitis virus. Cell.1987,48(3):363~364
[4] Rose J K.Schubert M.Genomes and their products.In Robert R , Wagner ed ,Rhabdovirues.New York:Plenum prees,1987.129-166
[5]殷震,刘景华.动物病毒学(第二版) [M].北京:科学出版社, 1997.11.
[6] Bourhy H,Molecular diversity of the lyssavirus genus,Virology,1993,194:70~81
[7] Richardsor JC,Peluso RW, Inhibition of VSV genome RNA replication but not tuanscription by monoclonal antibodies Specific for the viral P protein,Virology 1996 Feb 1;216(1):26~34
[8] Tordo N,Bourby H,Sather S, etal, Structure pression in bacular virus of the mookola virus glycoprotein, virology, 1993,184: 59~69
[9] Michael S N ,DM Hunt1Identificztion of an amino acid change that affects N protein function in resicular stomatitis virus [J]. Journal of General Virology,1994,75:3591– 3595
[10]Pierre Boudinot ,Sabine Riffault ,Samia Salhi ,et al1VesicularStomatitis Virus and pseudorabies virus induce a vigl/ cig5homdogue in mouse dendritic cells via different pathways [J ]1Journal of General Virology,2000,81:2675– 2682
[11]Josedel L,Barrera,Geoffrey J. Persistence of vesicular stomatitis virus NewJersey RNA in convalescent Hamsters [J ]. Virology,1996,219:453– 464
[12]Charlie D, Structrue of rhabdoviruses[J]. Virology,1999 (2):239– 260
[13]Das T,Gupta A K,Sims P W,et al. Role od cellular case in kinaseⅡin the function of the phosphoprotein(P) subunit of RNA polymerase of vesicular stomatitis virus[J]. The journal of biological chemistry,1995,27:4100– 4107.
[14]Von Kobbe ,et al1Molecular Biology of VSV[J ]1Molecular Cell12000 (6) :12431
[15]Grigera P R,Garcia - Briones M,Periolo O,et al1Immunogencity of an aphthovirus chimera of the glycoprotein of vesicular stomatitis virus[J ]1Journal of Virology ,1996 ,70(12) :8492– 85011
[16]Yang J,Hooper DC,Wunner WH et.al , The specificity of rabies virus RNA encapsidation by nucleoprotein Virology 1998,242;107~117
[17]Yoshiharu M,Hideki T,Kensuke D ,et al1Characterization of pseudotype VSV possessing HCV Envelpe proteins [J ]1Virology ,2001 ,286 :263– 2751
[18]Terstegen L,Gatsios P,Ludwig S,et.al The VSV M protein inhibits glycoprotein 130—dependentSTAT activation, J Immumol 2001 Nov I.167(9):5209~5216
[19]Michel lambed,Alain douart,Eliane joris,et al1Characterization of the innune response of cattle against non - cytopathic and cytopathic biotypes of bovine viral diarrhoea virus [J ]1Journal of General Virology,1997,78:1041–10471
[20]Schlegel R,Tralka TS,Willingham MC,et al. Inhibition of VSV binding and infectivity by phosphatidylserine is phosphatidylserine a VSV- binding site[J]. Cell. 1983,32:639–646.
[21]Schlegel R,Wade M. Neutralized vesicular stomatitis virus binds to host cells by a different "receptor" [J]. Biochem Biophys Res Commun. 1983, 114(2):774-778.
[22]Schlegel R,Willingham MC,Pastan IH. Saturable binding sites for vesicular stomatitis virus on the surface of Vero cells [J]. J Virol. 1982,43(3):871-875.
[23]David A,Coil,and A. Dusty Miller. Phosphatidylserine is not the cell surface receptor for vesicular stomatitis virus[J]. J. Virol. 2004,48(20):10920–10926
[24]Carneiro FA, Bianconi ML, Weissmuller G,et al. Membrane recognition by vesicular stomatitisvirus involves enthalpy-driven protein-lipid interactions[J]. J Virol. 2006,76(8):3756- 3764.
[25] Carneiro FA,Lapido-Loureiro PA,Cordo SM,et al. Probing the interaction between vesicular stomatitis virus and phosphatidylserine[J]. Eur Biophys J. 2006,35(2):145-154.
[26]Letchworth GJ,Rodriguez L L,Barrera J D C1 Vesicular Stomatitis[J ] The Veterinary Journal,1999,15:239 -260
[27]Angela Horn Montel,Grace Hommel- Berrey,Zacharie Brahmi1 Fas- Mediated cytotoxicity induces degradation of Vesicular Stomatitis Virus RNA transcripts and reduces virual titer [ J ] Molecular Immunology,1997,34 ( 15) 1055-10661
[28]Jack D K,Manfred S,Robert A L1 Interverning sequence between the leader region and the nucleocapsid gene of vesicular stomatitis virus RNA transcripts and reduces virual titer [J]. Journal of Virology,1980(5):789~794
[29]梁凤霞,丁明孝,翟中和水泡性口炎病毒的释放的方向性及其细胞骨架的关系[J].病毒学报,1993,9(2):167-170
[30]AhnnedM,Mckenzie MO,Puckettos,et.al, Ability of the Matrix protein of VSV to suppress beta interferon gene expression is genetically correlated with the inhibiton of host RNA and protein Syrthesis,J virol,2003 Apr;77(8):46~57
[31]Flanagan EB,Zamparo JM,Ball LA, et.al, Rearrangemont of the genes of VSV eliminates clinical disease in the natural host. J virol 2001 Jul,75(13):6107~6114
[32]Miller MA,lavine CL,Klas SD:Links Recombinant replication restricted vsv as an expression vector for murine cytokines,protein expr purify 2004.Jom 33(1):92~103
[33]Gupta AK,Banerjee AK, Expression and purification of VSV N-P complex from Es.col,J virol 1997 Jum 71(6):4264~4271
[34] Jayakar HR,whitt MA . Identification of two additional translation products from the matrix(M) gene than contribute to VSV cytopathology,J virol 2002,Aug;76(16):8011~8018
[35] Gaudier M,GaudinY,Cleavage of VSV M Protein prevents self-association and leads to crystallization[J]. Virology. 2001, 288(2):308~314
[36] Flangan EB,schoeb TR,Wertz Gw,VSV with rearranged genomes have altered invasiveness and neuropathogenesis in mice,J virol. 2003. 77(10):5740~5748
[37]Das T.pattnaik AK,Takacs AM et.al, Basic amino acid residues at the C-terminal eleven amino acid region of the P are required for transcription but not for replication of VSV genome RNA,Virology. 1997:238(1):103~114
[38]Whela SP,wertz GW,Regulation of RNA synthesis by the genomic termini of VSV,J Viro. 1999;73(1):297~306
[39]Mathieu ME,Grigera PR,Helenius A, et.al Folding,unfolding and refolding of the VSV glycoprotein,Biochemistry 1996. 35(13):4084~4093
[40]Da poian AT,Gomes AM.Oliveira RJ et.al,Migration of VSV glycoprotein to the nucleus of infected cells. Proc Nat I Acad Sci USA 1996 Aug 6;93(16):8268~8273
[41] Jayakar HR,MurtiKG,whitt MA, Mutations in the PPPY motif of vsv G Protein reduce virus budding by inhibiting a late step in virion release. J viral 2000 Nov;74(21):9818~9827
[42]Lyles DS,Mckenzie MO. Activity of VSV M protein mutants in cell rounding is correlated with the ability to inhibit host gene expression. Virology 1997 Mar 3;229(1):77~89
[43]Mathur M,Baner jee AK Novel binding of GTP to the P of VSV,Gene expr 2002;10(4):193~200
[44]Tordo N.Molecular epidemiology of lyssaviruses Focus on the glycoprotein and pseudogenes J vet . Res 1993,60:315~323
[45]靳喜朋,褚秀玲,霍惠玲.水泡性口炎的发病机制和临床诊治[J].张家口农专学报,2003,19 (2):8.
[46]黄运生.间接ELISA诊断水泡性口炎[J].中国兽医杂志,1989, 15 (4): 47.
[47]Ahmad S,Bassiri M,Banerjee AK,Yilma T. Immunological characterization of the VSV nucleocapsid (N) protein expressed by recombinant baculovirus in Spodoptera exigua larva:use in differential diagnosis between vaccinated and infected animals.
[48]Afshar A,Shakarchi N H,Dulac G C.Development of a competitive enzyme linked immunosorbent assay for detection of bovine,equine,ovine and porcine antibodies to vesicular stomatitis virus.J Clin Microbiol,1993,31:1860～1865
[49]Vernon S D , Webb P A , Recent vesicular stomatitis virus infection detected by immunoglobulin M antibody capture enzyme-linked immunosorbent assay.J Clin Microbiol,1985,22(4):582～586
[50]花群义,金宁一,徐自忠.水泡性口炎病毒核蛋白基因的表达及初步应用[J].生物工程学报,2004,20 (1):130.
[51]Rodriguez L L,Letchworth G J,Spiropoulou C F,Rapid detection ofvesicular stomatitis virus New Jerseyserotype in clinical samples by usingPCR[J ] . Journal of clinical microbiology,1993,(8):2016 - 2020.
[52]Hofner M C,Carpenter W C,Ferris N P A hemi - nested PCR assay for the detection and indentification of vesicular stomatitis virus nucleic acid. Journal of Virological Methods,1994,50:11-20.
[53]Ignacionunez,Blanco E,HernandezTA RT- PCR assay for the differential diagnosis of vesicular viral disease of swine[J ] . Journal of Virological Methods, 1998,72:227-235.
[54]Rasmussen TB,Uttenthal A,Fernández J,Storgaard T. Quantitative multiplex assay for simultaneous detection and identification of Indiana and New Jersey serotypes of vesicular stomatitis virus.J Clin Microbiol. 2005 Jan;43(1):356-362.
[55]花群义等;水泡性口炎检测技术体系的建立2005.12.24,科技成果
[56]杨桂梅,徐自忠. VSV的RT-PCR快速检测方法的建立[J].动物科学与动物医学,2003,20 (2):23.
[57]陈茹.水泡性口炎病毒血清型特异LUX实时荧光RT-PCR检测方法的建立.畜牧兽医学报. 2008, 39(4):522-528
[58]杨桂梅,徐自忠.二重RT-PCR同时检测VSV与BVDV核酸[J].中国预防兽医学报,2003,25 (4):291.
[59] Nunez JI,Blanco E,Hernandez T,et al. A RT-PCR assay for the differential diagnosis of vesicular viral diseases ofs wine [J]. J Virol Methods,1998,72 (2): 227.
[60] Reid SM,Ferris NP, Hutchings GH, et al. Primary diagnosisof foot-and-mouth disease by reverse transcription polymerase chain reaction [J]. J Virolo Methods, 2000,89 (1-2):167.
[61]徐自忠等,水泡性口炎、口蹄疫、猪水泡病和猪瘟检测技术,科技成果. 2005.12.11,
[62] Krumbholz A,Wurm R,Scheck O,et al. Detection of porcine teschoviruses and enteroviruses by LightCycler realtime PCR [J]. J Virol Methods, 2003, 113 (1):51.
[63] Reid SM,Ferris NP,Hutchings GH,et al. Evaluation of realtime reverse transcription polymerase chain reaction assays for the detection of swine vesicular disease virus[J]. J Virol Methods, 2004, 116 (2): 169.
[64] Afshar A, Dulac GC, Wright PF, et al. Application of indirect ELISA for detection of bovine antibodies against vesicular stomatitis viruses [J]. J Vet Diagn Invest, 1993, 5(1): 26.
[65]花群义等.水泡性口炎病毒核蛋白基因的克隆和序列分析.中国生物制品学杂志: 2004, 17 (1): 4.
[66]Katsunori Shinozaki,Oliver Ebert,Savio LC Woo,et al. Treatment of multifocal colorectal carcinoma metastatic to the liver of immune competent and syngeneic rats by hepatic artery infusion of oncolytic vesicular stomatitis virus.Cancer,2005,114(6):659～664
[67]David FS,Brian L,Shane K,et al. Exploiting tumor specific defects in the interferon pathway with apreviously unknown oncolytic virus.N at Med,2000,6(7):821～832
[68]Lee SB,Bablanian R,Esteban M,et al.Regulated expression of the Interferon induced protein kinase p68(PKR) by vaccinia virus recombinants inhibits the replication of vesicular stomatitis virus but not that of poliovirus.Interferon Cytokine Res,1996,16(12):1073～1084
[69]Jin HK,Takada A,Kon Y,et al.Identification of the murine Mx-gene:interferon induced expression of the Mx-protein from the feral mouse gene confers resistance to vesicular stomatitis virus.Virol,1999,73(6):4925～4932
[70]Steinhoff U,Muller U,Schertler A,et al. A ntiviral protection by vesicular stomatitis virusspecific antibodies in alpha/beta interferon receptor deficient mice.Virol,1995,69(4):2153～2163
[71] Balachandran S,Barber GN.Vesicular stomatitis virus(VSV) therapy of tumos. IUBMB Life,2000,50(2):135～138
[72]Siddharth B,Mercedes P,Glen N B,et al. Oncolytic activity of vesicular stomatitis virus is effective against tumors exhibiting Aberrant p53,Ras,or Myc function and involves the induction of apoptosis.Virol,2001,75(7): 3473～3479
[73] Ezelle H J,Markovic D,Barber G N,et al. Generation of Hepatitis C Virus-Like Particles by Use of a Recombinant Vesicular Stomatitis Virus Vector. Virol,2002,76(5):12325～12334
[74]Haglund K,Forman J,Kraèusslich H G,et al. Expression of Human Immunodeficiency Virus Type 1 Gag Protein Precursor and Envelope Proteins from a Vesicular Stomatitis Virus Recombinant:High-Level Production of Virus-like Particles Containing HIV Envelope. Virology,2000,26(8):112～121
[75]Kapadiaa S U,Rosea J K,Lamirande E.et al. Long-term protection from SARS coronavirus infection conferred by a single immunization with an attenuated VSV based vaccine.Virology,2005,340(3):174～182
[76]Publicover J,Ramsburg E ,Rose J K,et al. Asingle-cycle vaccine vector Based on vecicular etomamtitis virus can induce immune responses comparable to those generated by a replication copetent vector.Virol,2005,79(3):13231～13238
[77]Kretzschmar E,Buonocore L,Schnell M J,et al.High-Efficiency Incorporation of Functional Influenza Virus Glycoproteins into Recombinant Vesicular Stomatitis Viruses.Virol,1997,71(3):5982～5989
[78]Rajcani J. Molecular mechanisms of virus spread and virion components as tools of virulence [J]. Acta Microbiol Immunol Hung,2003, 50 (4): 407-443
[79]Norkin LC. Virus receptor: implications for pathogenesis and design of antiviral agents[J]. Clin Micro Rev, 1995,8(2):293
[80]Monaghan P, Gold S, Simpson J, et al. Theαvβ6integrin receptor for foot-and-mouth disease virus is expressed constitutively on the epithelial cells targeted in cattle[J]. J Gen Virol, 2005,86:2769
[81]Mendelsohn CL et al . Cell ,1989 ,56(10) :855—865
[82]Cattaneo R. Four viruses, two bacteria, and one receptor: membrane cofactor protein (CD46) as pathogens' magnet [J]. J Virol, 2004, 78(9): 4385-4388.
[83]Maurer K, Krey T, Moennig V, et al. CD46 is a cellular receptor for bovine viral diarrhea virus [J]. J Virol, 2004, 78 (4): 1792-1799.
[84]Masciopinto F,Campagnoli S,Abrignani S,et al. The sma llextracellular loop of CD81 is necessary for optimal surface expression of the large loop,a putative HCV receptor [J]. Virus Research,2001,80 (1-2):1-10.
[85]Sieczkarski SB,Whittaker GR. Virla entry[J]. Curr Top MicrobiolImmunol,2004,285:1
[86]Schols D. HIV co- receptor inhibitors as novel class of anti- HIV drugs[J]. Antiviral Res, 2006,71(3):216
[87]Jackson T,King A M,Stuart D I,et al. Structure and receptor binding. Virus Res, 2003,91 (1):33-46.
[88]Chen B, Vogan EM, Gong HY, et al. Determining the structure of an unliganded and fully glycosylated SIV gp120 envelope glycoprotein[J].Structure,2005,13(2):197
[89]Jackson T,King AM,Stuart D,et al. Structure and receptor binding [J]. Virus Res,2003,91:33
[90]Urban S, Breiner KM, Fehler F, et al. Avian hepatitis B virus infection is initiated by the interaction of a distinct pre-S subdomain with the cellular receptor gp180. J Virol. 1998, 72(10): 8089-8097.
[91]Schnell MJ, Buonocore L, Whitt MA, et al. The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. J Virol. 1996; 70(4): 2318-2323.
[92]Thomssen R, Bonk S, Propfe C, et al. Association of hepatitis C virus in human sera withβ-lipoprotein [J]. Med Microbiol Immunol, 1992, 181 (5): 293-300.
[93]Monazahian M, B?hme I, Bonk S, et al. Low density lipoprotein receptor as a candidate receptor for hepatitis C virus [J]. J Med Virol,1999, 57 (3):223-229.
[94]Agnello V,Abel G,Elfahal M, et al. Hepatitis C virus and other Flaviviridae viruses enter cells via low density lipoprotein receptor [J]. Proc Nat Acad Sci USA,1999,96 (22):12766-12771.
[95]Irina NG,Michael S,Robert S,et al .β3 integrin mediate the cellular entry of hantavirus that cause respiratory failure. Proc Natl Acad Sci USA,1998,95:7074-7079.
[96]Qing KY,Cathryn M,Jonathan H ,et al . Human fibroblast growth factor receptoel is a co2receptor for infection by adeno2associated virus 2. Nature medicine,1999,5:71-77.
[97]Deng HK,Liu Rong ,Wilfried E ,et al . Identification of a major co-receptor for primary isolates of HIV21. Nature,1996,381:6612666.
[98]Dalziel R G, Hopkins J, Watt N J, et al. Identification of a putative cellular receptor for the lentivirus visna virus [J]. J Gen Virol,1991,72 (Pt8):1905-1911.
[99]Tallet, B. et al. Expression,urification and biological properties of the carboxyl half part of the HTLV-I surface envelope glycoprotein [J]. J. of Chromatography B. 2000,737:85-95.
[100]Angus GD,Peter CLB,Paul RC,et al . The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature,1984,312:763-766.
[101]Maurer K,Krey T,Moennig V,et al. CD46 is a cellular receptor for bovine viral diarrhea virus [J]. J Virol,2004,78 (4):1792-1799.
[102]Dorig R E,Marcil A,Chopra A,et al. The human CD46 molecule is a receptor for measles virus (Edmonston strain) [J]. Cell,1993,75 (2):295–305.
[103]Yeager C L,Ashmun R A,Williams RK,et al. Human aminopeptidase N is a receptor for human coronavirus 229 E [J]. Nature,1992;357:420-422.
[104]Naniche D,Varior-Krishnan G,Cervoni F,et al. Human membrane cofactor protein (CD46) acts as a cellular receptor for measles virus [J]. J Virol,1993,67 (10):6025–6032.
[105]Hanham C A,Zhao F S,Tignor G H. Evidence from the anti-idiotypic network that the acetylcholine receptor is a rabies virus receptor [J]. J Virol,1993;67(1):530-542.
[106]Wang K S,Schmaljohn A L,Kuhn R J,et al. Anti-idiotypic antibodies as probes for the Sindbis virus receptor [J]. Virology,1991;181 (2);694-702.
[107]Haywood A M. .Virus receptors: binding, adhision strengthening and changes in viral structure [J]. Virol,1994,68(1):1-5.
[108]陆慧君,贺文琦,高丰,等.猪血凝性脑脊髓炎病毒PK细胞膜受体的鉴定[J].农业生物技术学报. 2008,16(1):20-23.
[109]Salas-Benito J S,Angel R M D.Identification of two surface proteins from C6/36 cells that bind dengue type4 virus[J]. J Virol,1997,71(10):7246-7252.
[110]尹崇,余健秀等.斜纹夜蛾多角体病毒包埋型受体的鉴定[J].中国病毒学. 2002, 17(1): 66-68.
[111]郭爱珍,陆承平.犬瘟热病毒细胞膜受体的鉴定[J].病毒学报,2000,16(2):155-157.
[112]Maisner A,Schneider-Schaulies J,Liszewski M K,et a1.Binding of measles virus to membrane cofactor protein(CD46):importance of disulfide bonds and N-glycans for the receptor function[J].J Virol,1994,68(10):6299-6304
[113]Gastka M Horvath J,Lentz TL.Rabies virus binding to the nicrotinic acetylcholine recepttor alpha subunit demonstrated by virus overly protein binding assay[J].J Gen Virol,1996,77(10):2437-2440
[114] Karger A,Mettenleiter T C.Identification of cell surface molecules that interact with pseudorabies virus[J]. J Virol. 1996,70(4):2138-2145
[115] Fields B N. Fundamental Virology 2nd ed. [M].New York: Raven Press,1990,87-95.
[116]Wu E,Trauger S A,Pache L,et al. Membrane cofactor protein is a receptor for adenoviruses associated with epidemic keratoc onjunctivitis [J]. J Virol,2004,78 (8):3897–3905.
[117] Haywood A M.. Virus receptors:binding,adhision strengthening,and changes in viral structure. J.Virol.,1994,68(1):1-5.
[118]Salas-Benito J S,Angel R M D.Identification of two surface proteins from C6/36 cells that bind dengue type4 virus[J]. J Virol,1997,71(10):7246-7252.
[119] Smith G P. Filamentous fusion phage:novel expression vectors that display cloned antigen on the virion surface [J]. Science. 1985,228:1315-1317.
[120]Paul P Sche,Kathleen M Mckenzie,et a1. Display cloning:fuctional identification of natural product receptors using cDNA-phage display[J]. Chemistry & Biology,1999,6:707-716.
[121]董倩,施双双,王业东,等. cDNA文库噬菌体展示法的建立及乙型肝炎病毒前S1蛋白结合蛋白筛检[J].解放军医学杂志. 2002, 4(27): 321-322.
[122]Hong S S,Karayan L,Tournier J,et al. Adenovirus type 5 fiber knob binds to MHC class Iα2 domain at the surface of human epithelial and B lymphoblastoid cells [J]. EMBO J,1997,16(9):2294-2306.
[123]Yu F, Christopher CB,Paul EK,et al. HIV-1 entry cofactor:functional cDNA cloning of a seven-transmembrane G protein-coupled receptor[J]. Science,1996, 272:872-877.
[124]Young JA,Bates P,Varmus HE.Isolation of a chicken gene that confers susceptibility to infection by subgroup A avian leukosis and sarcoma viruses[J] Virol. 1993,67(4):1811-1816.
[125]Greve JM Davis G, Meyer AM, et a1. The major human rhinovirus receptor is ICAM-1[J]. Cell,1989,56(5):839-847.
[126]Pileri P,Uematsu Y,Campagnoli S,et al .Binding of hepatitis C Virus to CD81. Science,1998,282 :338-341.
[127]Li WH,Moore MJ,Vasillieva N,et aL.Angiotension converting enzyme 2 is a functional receptor for SARS coronavirus[J].Nature,2003,426(6965):450-454.
[128]刘美德,赵彤言等. C6/36细胞上登革2型病毒受体的免疫共沉淀[J].中国病毒学. 2004,19(3):217-219.
[129]Ryu C J,Cho D Y,Gripon P,et al. An 80-kilodalton protein that binds to the Pre-S1 domain of hepatitis B virus [J]. J Virol,2000,74(1): 110-116.
[130]Fields S,Song O. A novalgenetic system to detect protein-protein interactions[J ]. Nature,1989,340:245-246.
[131]Li L Y,Liu X,Zhang P,et al. Cloning and functional identification of measles virus receptor on marmoset cells [J]. Chin Sci Bull,2002,47 (16):1217-1225.
[132]Aronheim A,Zandi E,Hennemann H,et al. Isolation of an AP-1 represso by a novel method for detecting protein-protein interactions[J]. Mol Cell Biol. 1997,17:3094-3102
[133]Johnsson N , V arshavsky A: Split ubiquitin as a sensor of protein interactions in vivo. Proc NatlA cad Sci USA 1994, 91: 10340-10344.
[134]Gaggar A,Shayakhmetov D M, Lieber A. CD46 is a cellular receptor for group B adenoviruses[J]. Nat Med,2003,9(11):1408-1412.
[135]Tio P H,Jong W W,Cardosa M J. Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1,2 and 3 [J]. Virology,2005.2 (1):25-36.
[136]Reyes-Del Valle J, Chavez-Salinas S, Medina F, et al. Heat shock protein 90 and heat shock protein 70 are components of dengue virus receptor complex in human cells[J]. J Virol,2005,79(8):4557-4567.
[137]Macbeath G,Schreiber SL. Printing proteins as microarrays for high-throughput function determination[J]. Science. 2000,289(5485):1673.
[138]王战会,靳刚.光学椭偏成像技术在生物分子研究中的应用[J].生物工程学报.2000,16(4):429-432.
[139] Jin G,Zhao ZY,Wang ZH et al. IEEE Conference ISIK2000 Workshop on Biomedical Information Engineering. Istanbul Turkey. 2000,137-140.
[140]Lofas S, et al. Bioanalysis with surface plasmon resenance,Sensor and Actuators,1991,B 5.:5-7
[141]Schreiber G. Kinetic studies of protein2protein interactions. Curr Opin Struct Biol ,2002 ,12(1) :41～47
[142] Schreiber G. Kinetic studies of protein2protein interactions. Curr Opin Struct Biol ,2002 ,12(1) :41～47
[143] Smith G P. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface [J]. Science, 1985, 228: 1315~1317.
[144] Dulbecco R, et al. US 1986, No. 4593002.
[145] Scott J K, Smith G P. Searching for peptide ligands with an epitope library [J].Science, 1990, 249: 386~390.
[146] Cwirla S E, Peters E A, Barrett R W, et al. Peptides on phage: A vast library of peptide for identifying ligands [J]. Proc Natl Acad Sci, USA, 1990, 87: 6378~6382.
[147] Devlin J J, Pangainban L C, Devlin P. Random peptide libraries: a source of specific protein binding molecules [J]. Science, 1990, 249: 404~406.
[148] Parmley J F, Smith G P, et al. Antibody-selectable filamentous fd phage vectors: affinity pacification of target genes [J]. Gene, 1988, 73: 305~318.
[149] Geysers H M, Rodda S J, Mason T J, et al. A priori delineation of a peptide which mimics a discontinuous antigenic determinant [J]. Immunol, 1986, 23: 709~715.
[150] McLafferty M A, Kent R B, L.adner R C, et al. M13 bacteriophage displaying disulfide-constrained microproteins [J]. Gene, 1993, 128: 29~36.
[151] Wang Y A, Yu X, Overman S, et al. The structure of a filamentous bacteriophage [J].J Mol Biol. 2006, 361(2): 209~215.
[152] Akita N, Maruta F, Sevmour L W, et al. Identification of oligo peptides binding toperitoneal tumors of gastric cancer [J]. Cancer Sci, 2006, 97 (10): 1075~1081.
[153] Sadanandam A, Varney M L, Kinarsky L, et al. Identification of Functional Cell Adhesion Molecules with a Potential Role in Metastasis by a Combination of in vivo Phage Display and in silico Analysis [J]. OMICS, 2007, Spring 11 (1): 41~57.
[154] Deroo S, Muller C P. Antigenic and immunogenic phage displayed mimotopes as substitute antigens: applications and limitations [J]. Combin Cheer Highthrouput Screen, 2001, 4: 75~110.
[155] Bartoli F, Nuzzo M, Urbanlli T, et al. DNA-based selection and screening of peptide ligands [J]. Nat. Biotechnol, 1998, 16: 1068~1073.
[156] Messmer B T, Benham C J, Thaler D S, et al. Sequential determination of ligands binding to discrete components in heterogeneous mixtures by interactive panning and blocking(IPAB) [J]. J Mol Biol, 2000, 296: 821~832.
[157]高鹏,黄秉仁.噬菌体展示文库及其应用[J].国外医学分子生物学分册, 2001, 23(6): 332-334.
[158]Benhar I. Biotechnological applications of phage and cell display [J]. Biotechnology Advances, 2001, 19 (1): 1~33.
[159] Benhar I, Azriel R, Nahary L, et al. Highly efficient selection of phageantibodies mediated by display antigen expression as LPP-OmpA′fusions on live bacteria [J]. J Mol Biol, 2000, 301 (4): 893~904.
[160] Ruud M T, de Wildt, Ian M Tomlinson, et al. Isolation of receptor-ligand pairs bycapture of long-lived multivalent interaction complexes [J]. Proc Natl Acad Sci USA, 2002, 99: 8530~8535.
[161]夏慧卿,邓洪宽,孙磊,崔国祯等,噬菌体展示文库筛选技术的研究进展,生命科学研究,2007 ,11 (1) : 50～53.
[162]Marco Antonio Arap. Phage display technology-Applications and innovations [J].Genetics and Molecular Biology, 2005, 28 (1): 1~9.
[163]赵慧斌,李晓军,武建国.噬菌体抗体库筛选技术[J].临床检验杂志, 2004, 22 (2) 46~148.
[164]Fukuda M N. Screening of Peptide-displaying phage libraries to identify short peptides mimicking carbohydrates [J]. Methods Enzymol, 2006, 416: 51~60.
[165]Aleksandr V, Pereboev 1, Naseem Ahmed, et al. Epitopes in the interacting regions of L-dystroglycan (PPxY motio and dystrophin (WW domain) [J]. Biochimica et Biophysica Acta, 2001, 1527: 54~60.
[166]Keresztessy Z, Csosz E, Harsfalvi J, et al. Phage display selection of efficient glutamine-donor substrate peptides for transglutaminase 2 [J]. Protein Sci, 2006, 15(11): 2466~2480.
[167]Koiwnen E, Arap W, Rajotte D. Identification of receptor ligands with phage display peptide libraries [J]. J Nuc Med, 1999, 40: 883~888.
[168]Awad A Osman, Holm Uhlig, Barbara Thamm, et al. Use of the phage display technique fordetection of epitopes recognized by polyclonal rebbit gliadin antibodies [J]. FEBS Letters, 1998, 433: 103~107.
[169]Ozawa M, Ohashi K, Onuma M. Identification and characterization of peptides binding to newcastle disease virus by phage display [J]. J Vet Med Sci, 2005, 67 (12):1237~1241.
[170]Mazzuccbelli L, BurritL B, Jesaitis A J, et al. Cell- specific peptide binding by human neutrophils [J]. Blood, 1999, 93 (5): 1738~1748.
[171]Kassner P D, Burg M A, Baird A, et al. Genetic selection of phage engineered for receptor- mediated gene transfer to mammalian cells [J]. Biochem Biophys Res Commun, 1999, 264 (3): 921~928.
[172]Pasqualini R, Ruoslahti E, et al. Organ targeting in vivo using phage display peptide libraries [J]. Nature, 1996, 380: 364~366.
[173]Rajotte D, Ruoslahti, et al. Membrane dipeptidase is the receptor for a lung-targeting peptide identified by in vivo phage display [J]. J Biol Chem, 1999, 274:11593~11598.
[174]Akita N, Maruta F, Sevmour L W, et al. Identification of oligo peptides binding to peritoneal tumors of gastric cancer [J]. Cancer Sci, 2006, 97 (10): 1075~1081.
[175]Sadanandam A, Varney M L, Kinarsky L, et al. Identification of Functional Cell Adhesion Molecules with a Potential Role in Metastasis by a Combination of in vivo Phage Display and in silico Analysis [J]. OMICS, 2007, Spring 11 (1): 41~57.
[176]Deroo S, Muller C P. Antigenic and immunogenic phage displayed mimotopes as substitute antigens: applications and limitations [J]. Combin Cheer Highthrouput Screen, 2001, 4: 75~110.
[177]Bartoli F, Nuzzo M, Urbanlli T, et al. DNA-based selection and screening of peptide ligands [J]. Nat. Biotechnol, 1998, 16: 1068~1073.
[178]Messmer B T, Benham C J, Thaler D S, et al. Sequential determination of ligands binding to discrete components in heterogeneous mixtures by interactive panning and blocking(IPAB) [J]. J Mol Biol, 2000, 296: 821~832.
[179]魏东芝,赖敏.噬菌体抗体库筛选技术[J].生命科学, 2000, 12 (3): 134~137.
[180]Benhar I. Biotechnological applications of phage and cell display [J]. Biotechnology Advances, 2001, 19 (1): 1~33.
[181] Benhar I, Azriel R, Nahary L, et al. Highly efficient selection of phageantibodies mediated by display antigen expression as LPP-OmpA′fusions on live bacteria [J]. J Mol Biol, 2000,301 (4): 893~904.
[182] Ruud M T, de Wildt, Ian M Tomlinson, et al. Isolation of receptor-ligand pairs by capture of long-lived multivalent interaction complexes [J]. Proc Natl Acad Sci USA,2002, 99: 8530~8535.
[183]刘志刚,俞炜源.新型噬菌体表面呈现载体的构建[J].生物技术通讯, 2002, 13 (1) : 50～53.
[184] Marco Antonio Arap. Phage display technology-Applications and innovations [J].Genetics and Molecular Biology, 2005, 28 (1): 1~9.
[185] Wind T, Stausbol G B, Kjaer S, et al. Retrieval of phage displayed scFv fragmentsusing direct bacterial elution [J]. J Immunol Methods, 1997, 209 (1): 75~83.
[186] Vatalit iA et al. Identification and characterization of peptides binding to newcastle disease virus by phage display.Cancer Res, 2000; 60 ( l6) : 4311
[187] Shane A et al. Cell-specific peptide binding by human neutrophils .Proc Natacad Sci USA , 1999; 96: 9497
[188] Daniel R et al. Genetic selection of phage engineered for receptor-mediated gene transfer to mammalian cells[J]. Biochem Biophys Res Commun, J Clin Invest, 1998; 102: 430
[189] MiChael S et al. Targeting in vivo using phage display peptide libraries J Biol Chem, 1997; 272(44) : 27943
[190]Parmley SF, Smith GP. dentification Identification of oligo peptides binding to peritoneal tumors of gastric cancer. Gene, 1988; 73: 305-318
[191]Kay BK. Antigenic and immunogenic phage displayed mimotopes as substitute antigens: applications and limitations Gene, 1993; 128: 59
[192] Cw irla SE. DNA-based selection and screening of peptide ligands Science. 1997; 274: l696
[193] Hoess RH. Sequential determination of ligands binding to discrete components in heterogeneous mixtures by iteractive panning and blocking. Biol, 1993; 3: 572
[194]Swimmer C et al. Phage Display of Ricin B Chain and its Single Binding Domains: System for Screening Galactose-Binding Mutants Proc N at l Acad Sci U SA , 1992; 89:
[195]SggioI et al. Highly efficient selection of phage antibodies mediated by display antigen expression as LPP-OmpA primer fusions on live bacteria.Gene, l995; 152: 35
[196]Choo Y et al. Isolation of receptor-ligand pairs by capture of longlived multivalentinteraction complexes Nature, 1994; 372: 642
[197]Chang TY. Transfusion, 2001; 41 (1) : 6
[198]周俊梅.噬菌体表面展示及其与疫苗研制有关的应用进展[J].国外医学.寄生虫病分册, 1998, 6, 241~246.
[199]Takagi T, Arisawa T, Yamamoto K, et al. Identification of ligands binding specifically to inflammatory intestinal mucosa using phage display [J]. Clin Exp Pharmacol Physiol, 2007, 34 (4): 286~289.
[200] Xu L, Jin B Q, Fan D M. Selection and identification of mimic epitopes for gastric cancer associated antigen MG7-Ag [J]. Cancer Ther, 2003, 2: 301-306.
[201] Romanov V I, Durand D B, Petrenko V A. Phage display selection of peptides that affect prostate carcinoma cells attachment and invasion [J]. Prostate, 2001, 47:239~251.
[202] Zhang Y, Chen J, Zhang Y et al. Panning and Identification of a Colon Tumor Binding Peptide from a Phage Display Peptide Library [J]. J Biomol Screen, 2007,[Epub ahead of print].
[203] Garamet ikoff K et al. Phage display technology-Applications and innovations Nucl Acids Res, 1994; 22; 5761
[204] Tanskanen J et al. Construction of a Multihybrid Display System: Flagellar Filaments Carrying Two Foreign Adhesive Peptides App l Environ M icrobio l, 2000; 66(9) : 4152
[205]沈霞芬主编,家畜组织学与胚胎学[M].中国农业出版社,第三版,2000.
[206] Wille JJ,Mansson-Rahemtulla B,Rahemtulla F.Characterization of human gingival eratinocytes culture in a serumfree medium[J].Arch Oral Biol,1990, 35(12):967-976.
[207]Oda D,Savard CE,Eng L,et al.Reconstituted human oral and esophageal mucosa in culture[J]. In Vitro Cell Dev Biol Anim,1998,34(1):46-52.
[208]Sugimura Y,Hata K,Torii S et al.Transplantation of cultured mucosal epithelium:an experimental study.J Craniomaxillofac Surg,1996,24 (6):352-359.
[209]Gibson P,Rosella O,Young G,et al.Serum free medium increases express- ion of markers of differentiation in human colonic crypt cells. Gut,1994,35(6):791-797.
[210]Reid C,Cloos J,Snow G,et al. A simple and reliable technique for culturing of human oral keratinocytes and fibroblast. Acta Otolaryngol, 1997,117:628-634.
[211]Ueda M,Hata K,Horie K,et al.The potential of oral mucosal cells for cultured epithelium:a preliminary report.Ann Plast Surg,1995,35 (5):498-504.
[212]Germain L,Rouabhia M,Guignard R,et al.Improvement of human keratinocyte isolation and culture using thermolysin.Burns,1993,19∶99.
[213]李宏卫,温玉明,王昌美,祁兵,等.人口腔黏膜上皮细胞的培养[J].南京医科大学学报,2003,23(4):379-381.
[214]Bergmann J E,Singer SJ.Immunoelectron microscopic studies of the intracellular transport of the membrane glycoprotein(G) of vesicular stomatitis virus in infected Chinese hamster ovary cells[J]. J Cell Biol,1983,97 (6):1777- 1787.
[215] Blumberg B M, Leppert M, Kolakofsky D. Interaction of Vesicular Stomatitis Virus leader RNA and nucleocapsid protein may control Vesicular Stomatitis Virus genome replication[J]. Cell,1981,23 (3):837– 845.
[216]王斌,李霞,高船舟.皱纹盘鲍一种球形病毒的感染及其发生[J].中国病毒学,1994,4(12):360-363.
[217] Ono K,Dubois-Dalcq M E, Schubert M et al.A mutated membrane protein of Vesicular Stomatitis Virus has an abnormal distribution within the infected cell and causes defective budding[J].J Viro1,1987,61(5):1332- 1341.
[218] Lisa D. Chong and John K. Rose . Membrane association of functional vesicular stomatitis virus matrix protein in vivo[J]. J. Virol.,1993;67:407-414.
[219] Brian D. Lichty,Heidi McBride,Stephen Hanson,et al. Matrix protein of Vesicular stomatitis virus harbours a cryptic mitochondrial-targeting motif[J]. J. Gen. Virol.,2006;87:3379-3384.
[220] Kalervo Metsikko and Kai SimonsThe budding mechanism of spikeless Vesicular Stomatitis virus particles[J].The EMBO Journal,1986,5(4):1913-1920.
[221] Jian Zhou and Gary W. Blissard. Display of Heterologous Proteins on gp64null Baculovirus Virions and Enhanced Budding Mediated by a Vesicular Stomatitis Virus G-Stem Construct[J]. J. Virol., 2008; 82: 1368-1377.
[222] Takacst A M, Banerjee A K. Inhibition of vesicular stomatitis virus in cells constitutively expressing an antisense RNA targeted against the virus RNA polymerase gene [J]. J Gen Virol.. 1997, 78(pt1): 125-129.
[223] Lambot M, Douart A, Joris E, et al. Characterization of the immune response of cattle against non-cytopathic and cytopathic biotypes of bovine viral diarrhoea virus [J]. J Gen Virol. 2006; 78 ( Pt 5):1041-1047.
[224] Schlegel R, Tralka TS, Willingham MC, et al. Inhibition of VSV binding and infectivity by phosphatidylserine: is phosphatidylserine a VSV- binding site[J]. Cell. 1983, 32:639–646.
[225] Schlegel R, Wade M. Neutralized vesicular stomatitis virus binds to host cells by a different "receptor" [J]. Biochem Biophys Res Commun. 1983, 114(2):774-778.
[226] Schlegel R, Willingham MC, Pastan IH. Saturable binding sites for vesicular stomatitis virus on the surface of Vero cells [J]. J Virol. 1982, 43(3): 871-875.
[227] David A, Coil, and A. Dusty Miller. Phosphatidylserine is not the cell surface receptor for vesicular stomatitis virus[J]. J. Virol. 2004, 48(20):10920–10926.
[228] Carneiro FA, Bianconi ML, Weissmuller G, et al. Membrane recognition by vesicular stomatitis virus involves enthalpy-driven protein-lipid interactions[J]. J Virol. 2006, 76(8): 3756- 3764.
[229] Carneiro FA, Lapido-Loureiro PA, Cordo SM, et al. Probing the interaction between vesicular stomatitis virus and phosphatidylserine[J]. Eur Biophys J. 2006, 35(2):145-154.