竹黄颗粒剂Ⅱ对银屑病患者IL-8和RANTES影响的研究
摘要
目的:观察竹黄颗粒剂Ⅱ号对趋化因子IL-8和RANTES的影响,探讨其作用机理。
方法:临床观察治疗共纳入60例,男33例,女27例,随机分为竹黄颗粒剂Ⅱ号治疗组(治疗组)和银屑灵冲剂治疗组(对照组)。采用ELISA法检测血清和皮损中IL-8及RANTES的含量。另纳入10名健康人为正常组。治疗前后分别进行银屑病面积与严重程度指数(PAIS)和皮肤病生活质量(DQOLS)的评估。
结果:1.竹黄颗粒剂Ⅱ治疗银屑病的有效率为93.3%,优于银屑灵的73.3%(P＜0.05)。2.竹黄颗粒剂Ⅱ号能较好改善银屑病人生活质量(P＜0.05)。3.竹黄颗粒剂Ⅱ号能较好缩小银屑病患者皮损面积,改善红斑、丘疹、鳞屑、瘙瘁症状(P＜0.05),优于银屑灵冲剂治疗组(P＜0.05)。4.竹黄颗粒剂Ⅱ号能减少血清及皮损中IL-8的含量(P＜0.05),优于银屑灵冲剂治疗组(P＜0.05)。血清中IL-8含量与皮损中IL-8含量成正相关。5.竹黄颗粒剂Ⅱ号能减少血清及皮损中RANTES的含量(P＜0.05),优于银屑灵冲剂治疗组(P＜0.05)。血清中RANTES含量与皮损中RANTES含量成正相关。
结论:竹黄颗粒剂Ⅱ号治疗用于治疗银屑病有较好的临床疗效。研究表明其能降低血液及皮损中趋化因子IL-8和RANTES含量,减轻局部炎症浸润,抑制角质形成细胞过度增殖,从而达到治疗银屑病的目的。
Objective: To investigate the mechanism of Zhu Huang Granule-II in the treatment of psoriasis vulgaris and to observe its effects on Chemokine IL-8 and RANTES.
Method: 60 suffers involed were divided into two groups, treated group (n=30) and control group(n=30) at random. The patients in treated group were treated with Zhu Huang Granule-II (ZHG-II), which in control group were treated with Yingxueling chong ji . The level of IL-8 and RANTES were detected by ELISA, both in sera and lesion samples, before and after treatment.10 healthy persons were tested also, acting as normal compare group. Psoriasis area and severity index(PAIS)and dermatosis quality of life statistical forms (DQOLS) were evaluated before and after treatment.
Results: 1. The level of IL-8 in sera and leision samples after treatment were lower than that of before treatment (P<0. 05). Treatment group was better than control group (P<0. 05). 2. The level of RANTES in sera and leision samples after treatment were lower than that of before treatment(P<0.05). Treatment group was better than control group(P<0.05). 3. PAIS index reduced(P<0.05). 4. DQOLS index reduced (P<0. 05).
Conclusion: It was suggested from the results that ZHG-II Zhu could reduce the level of IL-8 and RANTES both in sera and lesion samples. So that it can improve suffers' clinical symptoms and living quality.
方法:临床观察治疗共纳入60例,男33例,女27例,随机分为竹黄颗粒剂Ⅱ号治疗组(治疗组)和银屑灵冲剂治疗组(对照组)。采用ELISA法检测血清和皮损中IL-8及RANTES的含量。另纳入10名健康人为正常组。治疗前后分别进行银屑病面积与严重程度指数(PAIS)和皮肤病生活质量(DQOLS)的评估。
结果:1.竹黄颗粒剂Ⅱ治疗银屑病的有效率为93.3%,优于银屑灵的73.3%(P＜0.05)。2.竹黄颗粒剂Ⅱ号能较好改善银屑病人生活质量(P＜0.05)。3.竹黄颗粒剂Ⅱ号能较好缩小银屑病患者皮损面积,改善红斑、丘疹、鳞屑、瘙瘁症状(P＜0.05),优于银屑灵冲剂治疗组(P＜0.05)。4.竹黄颗粒剂Ⅱ号能减少血清及皮损中IL-8的含量(P＜0.05),优于银屑灵冲剂治疗组(P＜0.05)。血清中IL-8含量与皮损中IL-8含量成正相关。5.竹黄颗粒剂Ⅱ号能减少血清及皮损中RANTES的含量(P＜0.05),优于银屑灵冲剂治疗组(P＜0.05)。血清中RANTES含量与皮损中RANTES含量成正相关。
结论:竹黄颗粒剂Ⅱ号治疗用于治疗银屑病有较好的临床疗效。研究表明其能降低血液及皮损中趋化因子IL-8和RANTES含量,减轻局部炎症浸润,抑制角质形成细胞过度增殖,从而达到治疗银屑病的目的。
Objective: To investigate the mechanism of Zhu Huang Granule-II in the treatment of psoriasis vulgaris and to observe its effects on Chemokine IL-8 and RANTES.
Method: 60 suffers involed were divided into two groups, treated group (n=30) and control group(n=30) at random. The patients in treated group were treated with Zhu Huang Granule-II (ZHG-II), which in control group were treated with Yingxueling chong ji . The level of IL-8 and RANTES were detected by ELISA, both in sera and lesion samples, before and after treatment.10 healthy persons were tested also, acting as normal compare group. Psoriasis area and severity index(PAIS)and dermatosis quality of life statistical forms (DQOLS) were evaluated before and after treatment.
Results: 1. The level of IL-8 in sera and leision samples after treatment were lower than that of before treatment (P<0. 05). Treatment group was better than control group (P<0. 05). 2. The level of RANTES in sera and leision samples after treatment were lower than that of before treatment(P<0.05). Treatment group was better than control group(P<0.05). 3. PAIS index reduced(P<0.05). 4. DQOLS index reduced (P<0. 05).
Conclusion: It was suggested from the results that ZHG-II Zhu could reduce the level of IL-8 and RANTES both in sera and lesion samples. So that it can improve suffers' clinical symptoms and living quality.
引文
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2.佟长顺.中国皮肤性病学杂志.2002,3
3.邵长庚,张国家,包寅德.全国1984年银屑病调查报告.中华皮肤科杂志,1986,19:25-32
4.Tokura,et al.Arch Dermatol Res,1999,291(7-8):382
5.Yamaoto,et al.Eur J Dermatol,1999,9(1):17)
6.Bunse T,et al.Arch Dermatol Venereol(Stockh),1996,76(1):1
7.Tsuboi R,et al.Jpn J Med Myo01,1995,36(2):127
8.Weissenbom,et al.J Invest Dermatol,1999,113(1):122
9.Ametz MD,et al.Psycho ed,1985:47(6):528
10.Harvima RJ,et al.Acta Derm Vevereol(stockh),1996,76:467
11.J Koo JY,Do JH,Lee CS.Psychodermatology[J].J Am Acad Detmatol,2000,43(5 Pt 1):848
12.Harvima RJ,Viinamaki H,Hravima IT,et al.Association of psychic stress with clinical severity and symptoms of psoriatic patients[J].Acta Derm Venereol,1996,76(6):476
13.刘建新,危玲,李花.《黄帝内经》所论情志致病原因之我见.中医研究,2004,6:10
14.武刚.情志学说研究思路探析.安微中医学院学 报,2001,20(4):20-23
15.何文彬.黄帝内经心身疾病方法及其特点.中国医药学报,2000,15(6):22
16.董湘玉主编.中医心理学基础.北京:科学技术出版社,2003:126
17.黄跃东,李珀,赵俊芳.中医情志的发生机制刍议.福建中医学院学报.2004,4:43
18.严灿,徐志伟.肝主疏泄调畅情志功能的中枢神经生物学机制探讨.中国中西医结合杂志,2005,5:459
19.严灿,徐志伟,李艳.调肝健脾补肾方药对反复心理应激大鼠的中枢调整作用.广州中医药大学学报 2003;30(2):1431-1433
20.Pukuoka M,Ogino Y,Sato H,et al.RANTES expression in psoriatic skin,and regulation of RANTES and IL-8 production in cultured epidermal keratinocytes by active vitamin D3(tacalctol).Br J Detmatol,1998,138(1):63
21.杨志波,欧阳恒.竹黄颗粒剂治疗银屑病的临床研究.中国中医药信息杂志,2001,1:
22.杨志波,刘炽等.竹黄颗粒剂Ⅱ号对寻常性银屑病患者生活质量影响的研究.中国皮肤性病学杂志,2004,2:
23.杨志波,王军文等.竹黄颗粒剂对培养人角朊细胞增殖的影响.中国医师杂志,2001,3:
24.向亚平,陈晋文等.竹黄颗粒剂Ⅱ号对寻常性银屑病皮损MMP-2TIMP-2的影响.中国皮肤性病学杂志,2004,11
25.杨志波.竹黄颗粒剂Ⅱ号对应激小鼠皮肤肥大细胞影响的实验研究.湖南中医学院学报,2002,2:
26.李冠勇,刘华昌,尹格平,等.银屑病患者活化血小板的流式细胞分析.中华皮肤科杂志,1997,30(2):97
27.任晓丽,白莉.银屑病病患者血液流变学的研究.山西医药杂志,2004,10:862
28.吴一菲.120例银屑病患者精神情绪因素分析.中华今日医学杂.2003,1:25
29.王继湖,於如军,徐长忠.药物配合健康教育治疗银屑病58例疗效观察58例疗效观察.皮肤病与与性病,2005,2:18
30.熊抗美,赵志付,王彩凤.中医心身并治法在心身疾病康复中的应用.中国临床康复,2005,2:184
31.陶迪生.欧阳恒治疗泛发性脓疱型银屑病经验.湖南中医杂志.1998,4:41
32.钟金宝,殷新.禤国维教授治疗银屑病经验介绍.新中医.2004,4:11
1.北京中医医院.赵炳南临床经验集.北京:人民卫生出版社,1979:211
2.秦万章.皮肤病研究.上海:上海科学技术出版社,1990.326
3.顾伯华.中医外科临床手册.上海:上海科学技术出版社,1986.364
4.蒋蔚.从痹论治银屑病.四川中医,1999,17(12):14
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