不良心理应激对人卵巢癌裸鼠血清sIL-2R，VEGF，CA-125的影响
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摘要
目的：利用人卵巢上皮性癌荷瘤裸鼠建立卵巢癌不良心理应激模型,探讨不良心理应激对人卵巢上皮性癌荷瘤裸鼠血清可溶性白介素2受体(sIL-2R)、血管内皮生长因子(VEGF)、CA-125影响。
方法：将24只裸鼠随机分为4组：正常生长组(Ⅰ)、单纯应激组(Ⅱ)、单纯荷瘤组(Ⅲ)、荷瘤+应激组(Ⅳ),每组6只,建立相应的人卵巢上皮性癌荷裸鼠模型和不良心理应激模型。观察皮下瘤生长情况、裸鼠体重的变化,采用酶联免疫吸附法(ELISA)检测各组裸鼠血清中sIL-2R,CA-125,VEGF的含量。结果：Ⅳ裸鼠皮下瘤比Ⅲ生长较快,肿瘤增长率达66.33%。Ⅱ裸鼠血清中sIL-2R、VEGF水平与Ⅰ相比有显著性差异(P<0.05), sIL-2R、VEGF水平明显高于对照组(P<0.05)；Ⅱ裸鼠血清中CA-125水平与Ⅰ相比无显著性差异(P>0.05)。Ⅲ与Ⅰ,Ⅳ与Ⅰ相比sIL-2R、VEGF、CA-125水平均有显著性差异(P<0.05)。
结论：
1、成功建立了不良心理应激束缚模型。
2、不良心理应激使人卵巢癌荷瘤裸鼠肿瘤的瘤体重量明显增加。不良心理应激可导致裸鼠血清中血清中的SIL-2R、VEGF、CA125明显升高,说明不良心理应激可抑制荷瘤裸鼠的免疫功能,可能促进肿瘤快速增长。
Objective:To establish dys-psychological stress model using nude mice bearing human epithelium ovarian carcinoma.To investigate expression of Soluble Interleutin-2 Receptor(sIL-2R), vascular endothelial growth factor(VEGF) and CA-125 in human ovarian carcinoma of adverse psychological stress in nude mice.
Methods:All nude mice were randomized into 4 groups(each n=6):normal control group(Ⅰ),simple stress group(Ⅱ),tumor group(Ⅲ),tumor plus stress group(Ⅳ).Adverse psychological stress model and human ovarian carcinoma xenografs model were established in correlated groups. The growth of tumors were observed in nude mice.The weight of subcutaneous tumor were measured,the concentrations of sIL-2R,VEGF and CA-125 in serum were detected by enzyme-linked immunoabsorbent assay (ELISA).
Results:Compared with tumor group,the tumor grew faster in tumor plus stress group,the growth rate of which was 66.33%(P<0.05).The level of sIL-2R,VEGF in simple stress group was significantly higher than in normal control group(P<0.05). There was not significant difference in the expression of CA-125 level between simple stress group and normal control group (P>0.05).Tumor group and normal control group, tumor plus stress group and normal control group were significantly different when they were compared with correlated group(P<0.05),the concentrations of sIL-2R,VEGF and CA-125 in serum were significantly higher than correlated control group (P<0.05)
Conclusions:
1.Dys-psychological stress model with restraint was successfully established.
2.The dys-psychological stress adds the tumor weight significantly.The level of sIL-2R,VEGF and CA125 in tumor plus stress groupwas significantly higher than in normal control group.Adverse psychological stress can suppress the immune function of tumor-bearing nude mice, resulting in rapid growth of tumors.
方法：将24只裸鼠随机分为4组：正常生长组(Ⅰ)、单纯应激组(Ⅱ)、单纯荷瘤组(Ⅲ)、荷瘤+应激组(Ⅳ),每组6只,建立相应的人卵巢上皮性癌荷裸鼠模型和不良心理应激模型。观察皮下瘤生长情况、裸鼠体重的变化,采用酶联免疫吸附法(ELISA)检测各组裸鼠血清中sIL-2R,CA-125,VEGF的含量。结果：Ⅳ裸鼠皮下瘤比Ⅲ生长较快,肿瘤增长率达66.33%。Ⅱ裸鼠血清中sIL-2R、VEGF水平与Ⅰ相比有显著性差异(P<0.05), sIL-2R、VEGF水平明显高于对照组(P<0.05)；Ⅱ裸鼠血清中CA-125水平与Ⅰ相比无显著性差异(P>0.05)。Ⅲ与Ⅰ,Ⅳ与Ⅰ相比sIL-2R、VEGF、CA-125水平均有显著性差异(P<0.05)。
结论：
1、成功建立了不良心理应激束缚模型。
2、不良心理应激使人卵巢癌荷瘤裸鼠肿瘤的瘤体重量明显增加。不良心理应激可导致裸鼠血清中血清中的SIL-2R、VEGF、CA125明显升高,说明不良心理应激可抑制荷瘤裸鼠的免疫功能,可能促进肿瘤快速增长。
Objective:To establish dys-psychological stress model using nude mice bearing human epithelium ovarian carcinoma.To investigate expression of Soluble Interleutin-2 Receptor(sIL-2R), vascular endothelial growth factor(VEGF) and CA-125 in human ovarian carcinoma of adverse psychological stress in nude mice.
Methods:All nude mice were randomized into 4 groups(each n=6):normal control group(Ⅰ),simple stress group(Ⅱ),tumor group(Ⅲ),tumor plus stress group(Ⅳ).Adverse psychological stress model and human ovarian carcinoma xenografs model were established in correlated groups. The growth of tumors were observed in nude mice.The weight of subcutaneous tumor were measured,the concentrations of sIL-2R,VEGF and CA-125 in serum were detected by enzyme-linked immunoabsorbent assay (ELISA).
Results:Compared with tumor group,the tumor grew faster in tumor plus stress group,the growth rate of which was 66.33%(P<0.05).The level of sIL-2R,VEGF in simple stress group was significantly higher than in normal control group(P<0.05). There was not significant difference in the expression of CA-125 level between simple stress group and normal control group (P>0.05).Tumor group and normal control group, tumor plus stress group and normal control group were significantly different when they were compared with correlated group(P<0.05),the concentrations of sIL-2R,VEGF and CA-125 in serum were significantly higher than correlated control group (P<0.05)
Conclusions:
1.Dys-psychological stress model with restraint was successfully established.
2.The dys-psychological stress adds the tumor weight significantly.The level of sIL-2R,VEGF and CA125 in tumor plus stress groupwas significantly higher than in normal control group.Adverse psychological stress can suppress the immune function of tumor-bearing nude mice, resulting in rapid growth of tumors.
引文
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[3]李凌江,李则宣.精神应激的生物学致病机制研究一Ⅱ：神经内分泌机制.中国行为医学科学,2005,14：156-158.
[4]任宏轩,沈丽达.社会心理因素对癌症的影响[J].中国肿瘤,2005,14(10)：639-641.
[5]TomatisL.Between the body and the mind the involvement of Psychological factors in the deve 1 opment of mu 1 tifactoiral diseases [J]EurJ Cancer,2001,37(85):148-152.
[6]范少光,查宏斌,蒋永刚,等.束缚应激小鼠血中产生的免疫抑制因子及其调节[J].科学通报,1989,20：1584-1587.
[7]陈易新,王天芳,季绍良,等.慢性束缚致大鼠慢性疲劳动物模型的肾上腺皮质超微结构的变化[J].北京中医药大学学报,2000,23(1)：33-35.
[8]Amy NS,Andrew JS,ColinR Y,et al.Chronic restraint stress during early Theiler's virus infection exacerbates the subsequent demyelinating disease in SJL mice[J].J Neuroimmunol, 2004,155(1-2):103-118.
[9]Ortiz,fitagerald L W, Lane S,etal.Biochemical adaptations in the mesolimbic dopamine system in response to repeated stress[J].Neurochopharmarcology,1996,14:443-452.
[10]Masashi Nibuya,Michihiro Takahashi,David S. Russell,et al.Repeated stress increases catalytic TrkB mRNA in rat hippocampus[J].Neuroscience,1999,267 (2):81-84.
[11]严灿,邓中炎,王剑.调肝方对慢性束缚应激大鼠神经内分泌免疫功能的影响[J].中国免疫学杂志,2000,16(9)：488-490.
[12]张沁园,刘延迟,陈利国.肝火疏泄对小肠吸收功能影响的实验研究[J].北京中医药大学学报,1999,5(5)：25-28.
[13]Maryam Al-Mohaisen,Arturo,Cardounel,Mohammed Kalimi. Repeated immobilizationstress increases total cytosoli glucocorticiod receptor in rat liver[J].Steriod,2000,65 (1):8-15.
[14]李蕾.慢性束缚应激和交感神经切除对大鼠胃酸分泌及血清促胃液素水平的影响[J].河南医科大学学报,1998,33(1)：28-29.
[15]刘恩岐.医学实验动物学[M].北京：人民卫生出版社,2004：169.
[16]孙慧,战患利,黄建英.等.人卵巢癌棵鼠移植瘤模型的建立及其生物学特性的实验应用研究[J)中国肿瘤临床,1994.21(4)：786.
[17]Fu X, Hoffman RM. Human ovarian carcinoma mettataticmodels constructed in nude mice by orthotopic transphn-ration of histologically intact patient specimens CJ]. Anti·cancer Res, 1993.139(2):283.
[18]Rygaard J, Povlsen CO. Heterotransplantation of a human malignant tumour to"Nude" mice[J]. Acta PatholMicrobiol Stand,1969,77(4):758-760.
[19]高国兰,邹春芳等.人卵巢上皮癌裸鼠皮下移植瘤模型的建立及生物学性状的鉴定[J].实用癌症杂志,2004,19(5)：454-457.
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