奶牛产后生殖机能恢复与生长抑素基因免疫
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摘要
繁殖力和泌乳性能是反映奶牛生产性能的重要因素,阐明奶牛生殖系统发育／恢复规律,对于最大限度发挥奶牛繁殖潜能、开发繁殖新技术具有重要的意义；而利用现代生物技术,提高奶牛泌乳水平,是提高奶牛养殖经济效益的关键。本研究首先利用B型超声波诊断技术研究不同胎次奶牛产后子宫复旧与卵巢机能恢复的规律,为提高奶牛产后繁殖力进而促进奶牛生产提供理论依据；之后利用基因免疫技术研究生长抑素基因免疫对奶牛生殖机能恢复和泌乳性能的作用,为开发提高奶牛繁殖力和泌乳力的基因疫苗奠定基础。在用新型生长抑素DNA疫苗——pVGS/2SS-asd免疫奶牛前,首先利用大鼠模型进行试验,探讨其对大鼠繁殖及泌乳性能的影响,并对其可能存在的安全性问题进行评价,为应用大动物(如奶牛)提供依据。然后将pVGS/2SS-asd生长抑素DNA疫苗免疫产后奶牛,研究其对奶牛产后繁殖及泌乳性能的影响。
1.奶牛产后子宫复旧与卵巢机能恢复规律研究
良好的子宫复旧和卵巢活动对产后奶牛再次受孕并缩短产犊间隔是非常重要的。本研究的目的是在产后营养和泌乳条件相对一致的情况下,评估胎次对中国荷斯坦奶牛产后子宫复旧和卵巢活动恢复的影响。选取46头中国荷斯坦奶牛,其中初产18头、产第二胎13头、经产15头。奶牛产后子宫和卵巢检测采用超声波诊断技术,干物质摄入量(DMI)、产奶量、体况评分(BCS)测定依据DHI(奶牛群体改良)标准方法,奶样中雌二醇浓度(E2)测定采用放射免疫测定(RIA)方法。结果显示,初产奶牛的子宫孕角恢复时间(天数)显著多于其他胎次的奶牛。就产后首次和第二次排卵的间隔天数及产后首次排卵前的卵泡波数目而言,初产奶牛也比其他胎次奶牛多。总之,中国荷斯坦奶牛在相似的营养和泌乳条件下,随着胎次的增长,奶牛产后子宫复旧与卵巢活动恢复时间明显缩短。
2.生长抑素基因免疫对大鼠繁殖和泌乳性能的影响
为了探讨新型生长抑素DNA疫苗——pVGS/2SS-asd对大鼠繁殖和泌乳性能的影响,同时分析疫苗剂量和注射方式对免疫效果的影响,将216只SD雌性大鼠随机分为9组,每组24只。试验组：T1～T3组大鼠按高(2×109 CFU)、中(2×108CFU)、低(2×107CFU)三种剂量进行股四头肌两点注射pVGS/2SS-asd重组菌200μL; T4～T6组所用疫苗及其用量对应于T1～T3组,进行背部皮下注射。对照组：C0组注射10%铝胶生理盐水(肌肉注射和皮下注射各半)；C1组肌肉注射2×109CFU不含质粒的C500菌200μL；C2组皮下注射2×109CFU不含质粒的C500菌200μL。各组在首次免疫后间隔4周,用同等剂量疫苗进行加强免疫2次。结果表明,这种新型pVGS/2SS-asd生长抑素DNA疫苗对大鼠有很好的免疫原性,能诱导高水平的免疫应答,而且通过加强免疫能在整个试验期保持较长时间(约11～13周)。在免疫方式上,肌肉注射在诱导产生抗体水平的绝对值(OD=0.823～0.075)和免疫应答的持久性(约13周)方面比皮下注射有优势。在免疫剂量上,并不存在剂量依赖关系,两种免疫方式均以中剂量优势明显。无论是肌肉注射或皮下注射,三种免疫剂量都对大鼠繁殖性能没有影响,但间接促进了产后泌乳期仔鼠的生长,改善了大鼠的泌乳性能。其中以肌肉注射的中剂量组的仔鼠断奶窝增重(215.8±18.8g)明显高于其他各组,与对照组相比差异显著。皮下注射的中剂量组次之(212.2±13.6g)。
3.生长抑素基因疫苗染色体整合的安全性评价
在用pVGS/2SS-asd生长抑素DNA疫苗肌肉注射和皮下注射SD大鼠试验结束时,采集各组母鼠的心、肝、脾、肺、肾、卵巢和肌肉以及断奶仔鼠的心、肝、脾、肺和肾等组织,提取基因组DNA,采用PCR方法检测质粒DNA与宿主染色体整合情况。结果表明,未发现质粒整合至母鼠和仔鼠基因组DNA中,揭示生长抑素DNA疫苗在染色体整合方面对大鼠是安全的。
4.生长抑素基因免疫对奶牛繁殖和泌乳性能的影响
为了探讨新型生长抑素DNA疫苗——pVGS/2SS-asd对产后奶牛繁殖和泌乳性能的影响,将40头产后奶牛随机分为4组,每组10头。3个试验组奶牛从奶牛产后第7d开始,分高(1×1011CFU)、中(1×1010CFU)、低(1×109CFU)三种剂量肌肉注射pVGS/2SS-asd重组菌1mL,对照组肌肉注射10%铝胶生理盐水1mL。首次免疫(注射当天0d)后间隔4周,用同等剂量疫苗加强免疫2次。结果表明,pVGS/2SS-asd生长抑素DNA疫苗对奶牛有很好的免疫原性,各剂量组均能诱导一定强度的免疫应答。高剂量组在诱导产生抗体最大峰值(OD=0.490±0.171)和抗体阳性牛比例的维持(100.0%持续7周左右)上优势明显。免疫后各组奶牛子宫颈和子宫孕角复旧时间、首次排卵时间和排卵卵泡最大直径等方面差异不显著,说明该疫苗对奶牛的繁殖性能没有不良影响,而对泌乳性能有一定的促进作用,其中以中剂量组奶牛优势明显,在首次免疫后有5周时间(第2～6周)产奶量明显高于其他组。
Reproduction and lactation are important factors which reflect the performance of dairy cattle. The explanation of the development/resumption laws of cows'reproductive system plays a significant role in the full development of the potential for cows' reproduction and new reproductive technologies. Modern biotechnology has been used to improve the milk level of Dairy cows, which is the key to improving the economic efficiency of dairy farms. The first part of this study is to investigate the law of uterine involution and ovarian function resumption of Chinese Holstein dairy cows with different parities by B-mode ultrasound during postpartum, so as to provide the research basis for improving the fertility of postpartum dairy cows. The second part is to study the role of somatostatin gene immunization on reproductive function recovery and lactation performance of dairy cows by genetic immunization technology, and to provide the basis for the development of genetic vaccine which improve the fertility and lactation of dairy cows. A novel somatostatin DNA vaccine—pVGS/2SS-asd was tested on the rat model prior to the dairy cow, and used to explore the effects on the fertility and lactation performance of rats. The safety issue of this DNA vaccine on rats was also evaluated. These results provided the basis for the application of large animals (such as dairy cows). Then postpartum dairy cows were immunized against the pVGS/2SS-asd somatostatin DNA vaccine, exploring the effects on the fertility and lactation performance of the postpartum dairy cows.
1. Study of uterine involution and resumption of ovarian activities in postpartum dairy cows with different parities.
Favorable uterine involution and ovarian activity are very important for the next pregnancy and the shorter calving interval of postpartum cows. The objective of this study was to evaluate the effect of parity on uterine involution and resumption of ovarian activity in Chinese Holstein dairy cows after calving under similar postpartum nutritional and lactational conditions.46 Chinese Holstein dairy cows in various parities (primiparous=18; biparous=13; multiparous= 15) were selected. The status of uterus and ovaries was detected by ultrasonography. According to DHI (Dairy Herd Improvement) standard method, dry matter intake (DMI), milk yield, body condition score (BCS) were determined, and estradiol (E2) concentration in milk samples were measured by radioimmunoassay (RIA). The results showed that days of the previous gravid uterine horn involution were significantly greater in primiparous dairy cows than in biparous and multiparous dairy cows. Days from calving to ovulation (first and second) and the number of follicular waves to first ovulation were also significantly greater in primiparous cows than in multiparous cows. In summary, under similar postpartum nutritional and lactational conditions, postpartum uterine involution and resumption of ovarian activity became progressively shorter as the number of parity increased in Chinese Holstein cows
2.The effects of immunization against a novel somatostatin DNA vaccine—pVGS/2SS-asd on fertility and lactation in female rats.
In order to investigate the effects of immunization against a novel somatostatin DNA vaccine—pVGS/2SS-asd on fertility and lactation in female rats, and analyze the effects of immunization doses and delivery routes on immune effect of this DNA vaccine,216 female Sprague-Dawley rats were randomly divided into 9 groups,24 per group. Test groups:rats of T1-T3 groups were primarily intramuscular immunized against pVGS/2SS-asd in high (2×109 CFU/200μL), middle (2×108 CFU/200μL) or low (2×107 CFU/200μL) dose; rats of T4-T6 groups in the same dosage as T1-T3 groups were primarily subcutaneous immunized against pVGS/2SS-asd s. Control groups:rats of CO group (half intramuscular injection and half subcutaneous injection) were immunized against 10% aluminum hydroxide gel saline (200μL); rats of C1 group were intramuscular immunized against C500 (2×109 CFU/200μL); rats of C2 group were subcutaneous immunized against C500 (2×109 CFU/200μL). Booster immunizations in the same dosage as the primary immunization were performed twice at 4-week intervals. The results showed that this novel pVGS/2SS-asd somatostatin DNA vaccine had good immunogenicity, induced a high level of immune response, and maintained a longer time (approximately 11-13 ws) by booster immunization in female rats. In the delivery routes, intramuscular immunity in the antibody absolute value (OD= 0.823±0.075) and persistent immune response (approximately 13 ws) was more advantages than subcutaneous immunity. In dose effect, there was no dose-dependent among all tests, and middle dose groups of two delivery routes were obvious advantages than other dose groups. Whether intramuscular or subcutaneous, the reproductive performance of all rats immunized with different doses was not affected. However, pVGS/2SS-asd somatostatin DNA vaccine in postpartum lactating rats contributes to the growth of offspring, and improves the lactation performance of female rats indirectly. Weaning weight gain (215.8±18.8 g) of offspring of the middle dose intramuscular group was significantly higher than those of other groups, with a significant difference between the middle dose intramuscular group and the control group, and followed by the middle dose subcutaneous group (212.2±13.6 g). 3.Safety evaluation of somatostatin DNA vaccine in chromosomal integration.
At the end of immunization against pVGS/2SS-asd somatostatin DNA vaccine in rats with intramuscular or subcutaneous injections, multiple tissues such as heart, liver, spleen, lung, kidney, ovary and muscle were removed from each female rat of test groups, respectively. Moreover, tissue samples of the pups, such as heart, liver, spleen, lung and kidney were also collected after weaning, respectively. The genomic DNA was extracted, and then the genomic DNA samples were detected by sensitive PCR method. The results showed that the plasmid was not integrated into maternal and offspring genomic DNA, and the result revealed that the somatostatin DNA vaccine in chromosomal integration was safe to rats.
4. Effects of Somatostatin gene immunization on the reproduction and lactation performance of dairy cows.
The purpose of this study was to explore the effects of the new somatostatin DNA vaccine—pVGS/2SS-asd on the reproduction and lactation performance of postpartum dairy cows.40 postpartum cows were randomly divided into 4 groups,10 per group. At 7d after calving,3 test group cows were primarily intramuscular immunized against pVGS/2SS-asd in high (1×1011 CFU/1mL), the middle (1×1010CFU/1mL), and low (1×109 CFU/1mL) dose; the control group cows were intramuscular immunized against 10% aluminum hydroxide gel saline(1mL). Booster immunizations in the same dosage as the primary immunization (injection day as 0 d) were performed twice at 4-week intervals. The results showed that the novel pVGS/2SS-asd somatostatin DNA vaccine for cows had good immunogenicity and could induce a certain intensity of immune response. High dose group in the induction of the maximum antibody peak value (OD= 0.490±0.171) and the persistent high proportion of antibody positive cattle (100.0% for 7 w) had obvious advantages than other dose groups. After immunization, there was no significant difference among all the groups in the gravid uterine horn and uterine cervical involution, intervals of first ovulation and ovulation follicle diameter, this somatostatin DNA vaccine did not adversely affect the reproductive performance of dairy cows. Moreover it also confirmed that in the similar nutritional conditions, this vaccine improved the lactation performance of cows. The middle dose group had more advantages than other dose groups. Milk yields of the middle dose group were significantly higher than other groups for 5 weeks (2w to 6w) after primary immunization.
1.奶牛产后子宫复旧与卵巢机能恢复规律研究
良好的子宫复旧和卵巢活动对产后奶牛再次受孕并缩短产犊间隔是非常重要的。本研究的目的是在产后营养和泌乳条件相对一致的情况下,评估胎次对中国荷斯坦奶牛产后子宫复旧和卵巢活动恢复的影响。选取46头中国荷斯坦奶牛,其中初产18头、产第二胎13头、经产15头。奶牛产后子宫和卵巢检测采用超声波诊断技术,干物质摄入量(DMI)、产奶量、体况评分(BCS)测定依据DHI(奶牛群体改良)标准方法,奶样中雌二醇浓度(E2)测定采用放射免疫测定(RIA)方法。结果显示,初产奶牛的子宫孕角恢复时间(天数)显著多于其他胎次的奶牛。就产后首次和第二次排卵的间隔天数及产后首次排卵前的卵泡波数目而言,初产奶牛也比其他胎次奶牛多。总之,中国荷斯坦奶牛在相似的营养和泌乳条件下,随着胎次的增长,奶牛产后子宫复旧与卵巢活动恢复时间明显缩短。
2.生长抑素基因免疫对大鼠繁殖和泌乳性能的影响
为了探讨新型生长抑素DNA疫苗——pVGS/2SS-asd对大鼠繁殖和泌乳性能的影响,同时分析疫苗剂量和注射方式对免疫效果的影响,将216只SD雌性大鼠随机分为9组,每组24只。试验组：T1～T3组大鼠按高(2×109 CFU)、中(2×108CFU)、低(2×107CFU)三种剂量进行股四头肌两点注射pVGS/2SS-asd重组菌200μL; T4～T6组所用疫苗及其用量对应于T1～T3组,进行背部皮下注射。对照组：C0组注射10%铝胶生理盐水(肌肉注射和皮下注射各半)；C1组肌肉注射2×109CFU不含质粒的C500菌200μL；C2组皮下注射2×109CFU不含质粒的C500菌200μL。各组在首次免疫后间隔4周,用同等剂量疫苗进行加强免疫2次。结果表明,这种新型pVGS/2SS-asd生长抑素DNA疫苗对大鼠有很好的免疫原性,能诱导高水平的免疫应答,而且通过加强免疫能在整个试验期保持较长时间(约11～13周)。在免疫方式上,肌肉注射在诱导产生抗体水平的绝对值(OD=0.823～0.075)和免疫应答的持久性(约13周)方面比皮下注射有优势。在免疫剂量上,并不存在剂量依赖关系,两种免疫方式均以中剂量优势明显。无论是肌肉注射或皮下注射,三种免疫剂量都对大鼠繁殖性能没有影响,但间接促进了产后泌乳期仔鼠的生长,改善了大鼠的泌乳性能。其中以肌肉注射的中剂量组的仔鼠断奶窝增重(215.8±18.8g)明显高于其他各组,与对照组相比差异显著。皮下注射的中剂量组次之(212.2±13.6g)。
3.生长抑素基因疫苗染色体整合的安全性评价
在用pVGS/2SS-asd生长抑素DNA疫苗肌肉注射和皮下注射SD大鼠试验结束时,采集各组母鼠的心、肝、脾、肺、肾、卵巢和肌肉以及断奶仔鼠的心、肝、脾、肺和肾等组织,提取基因组DNA,采用PCR方法检测质粒DNA与宿主染色体整合情况。结果表明,未发现质粒整合至母鼠和仔鼠基因组DNA中,揭示生长抑素DNA疫苗在染色体整合方面对大鼠是安全的。
4.生长抑素基因免疫对奶牛繁殖和泌乳性能的影响
为了探讨新型生长抑素DNA疫苗——pVGS/2SS-asd对产后奶牛繁殖和泌乳性能的影响,将40头产后奶牛随机分为4组,每组10头。3个试验组奶牛从奶牛产后第7d开始,分高(1×1011CFU)、中(1×1010CFU)、低(1×109CFU)三种剂量肌肉注射pVGS/2SS-asd重组菌1mL,对照组肌肉注射10%铝胶生理盐水1mL。首次免疫(注射当天0d)后间隔4周,用同等剂量疫苗加强免疫2次。结果表明,pVGS/2SS-asd生长抑素DNA疫苗对奶牛有很好的免疫原性,各剂量组均能诱导一定强度的免疫应答。高剂量组在诱导产生抗体最大峰值(OD=0.490±0.171)和抗体阳性牛比例的维持(100.0%持续7周左右)上优势明显。免疫后各组奶牛子宫颈和子宫孕角复旧时间、首次排卵时间和排卵卵泡最大直径等方面差异不显著,说明该疫苗对奶牛的繁殖性能没有不良影响,而对泌乳性能有一定的促进作用,其中以中剂量组奶牛优势明显,在首次免疫后有5周时间(第2～6周)产奶量明显高于其他组。
Reproduction and lactation are important factors which reflect the performance of dairy cattle. The explanation of the development/resumption laws of cows'reproductive system plays a significant role in the full development of the potential for cows' reproduction and new reproductive technologies. Modern biotechnology has been used to improve the milk level of Dairy cows, which is the key to improving the economic efficiency of dairy farms. The first part of this study is to investigate the law of uterine involution and ovarian function resumption of Chinese Holstein dairy cows with different parities by B-mode ultrasound during postpartum, so as to provide the research basis for improving the fertility of postpartum dairy cows. The second part is to study the role of somatostatin gene immunization on reproductive function recovery and lactation performance of dairy cows by genetic immunization technology, and to provide the basis for the development of genetic vaccine which improve the fertility and lactation of dairy cows. A novel somatostatin DNA vaccine—pVGS/2SS-asd was tested on the rat model prior to the dairy cow, and used to explore the effects on the fertility and lactation performance of rats. The safety issue of this DNA vaccine on rats was also evaluated. These results provided the basis for the application of large animals (such as dairy cows). Then postpartum dairy cows were immunized against the pVGS/2SS-asd somatostatin DNA vaccine, exploring the effects on the fertility and lactation performance of the postpartum dairy cows.
1. Study of uterine involution and resumption of ovarian activities in postpartum dairy cows with different parities.
Favorable uterine involution and ovarian activity are very important for the next pregnancy and the shorter calving interval of postpartum cows. The objective of this study was to evaluate the effect of parity on uterine involution and resumption of ovarian activity in Chinese Holstein dairy cows after calving under similar postpartum nutritional and lactational conditions.46 Chinese Holstein dairy cows in various parities (primiparous=18; biparous=13; multiparous= 15) were selected. The status of uterus and ovaries was detected by ultrasonography. According to DHI (Dairy Herd Improvement) standard method, dry matter intake (DMI), milk yield, body condition score (BCS) were determined, and estradiol (E2) concentration in milk samples were measured by radioimmunoassay (RIA). The results showed that days of the previous gravid uterine horn involution were significantly greater in primiparous dairy cows than in biparous and multiparous dairy cows. Days from calving to ovulation (first and second) and the number of follicular waves to first ovulation were also significantly greater in primiparous cows than in multiparous cows. In summary, under similar postpartum nutritional and lactational conditions, postpartum uterine involution and resumption of ovarian activity became progressively shorter as the number of parity increased in Chinese Holstein cows
2.The effects of immunization against a novel somatostatin DNA vaccine—pVGS/2SS-asd on fertility and lactation in female rats.
In order to investigate the effects of immunization against a novel somatostatin DNA vaccine—pVGS/2SS-asd on fertility and lactation in female rats, and analyze the effects of immunization doses and delivery routes on immune effect of this DNA vaccine,216 female Sprague-Dawley rats were randomly divided into 9 groups,24 per group. Test groups:rats of T1-T3 groups were primarily intramuscular immunized against pVGS/2SS-asd in high (2×109 CFU/200μL), middle (2×108 CFU/200μL) or low (2×107 CFU/200μL) dose; rats of T4-T6 groups in the same dosage as T1-T3 groups were primarily subcutaneous immunized against pVGS/2SS-asd s. Control groups:rats of CO group (half intramuscular injection and half subcutaneous injection) were immunized against 10% aluminum hydroxide gel saline (200μL); rats of C1 group were intramuscular immunized against C500 (2×109 CFU/200μL); rats of C2 group were subcutaneous immunized against C500 (2×109 CFU/200μL). Booster immunizations in the same dosage as the primary immunization were performed twice at 4-week intervals. The results showed that this novel pVGS/2SS-asd somatostatin DNA vaccine had good immunogenicity, induced a high level of immune response, and maintained a longer time (approximately 11-13 ws) by booster immunization in female rats. In the delivery routes, intramuscular immunity in the antibody absolute value (OD= 0.823±0.075) and persistent immune response (approximately 13 ws) was more advantages than subcutaneous immunity. In dose effect, there was no dose-dependent among all tests, and middle dose groups of two delivery routes were obvious advantages than other dose groups. Whether intramuscular or subcutaneous, the reproductive performance of all rats immunized with different doses was not affected. However, pVGS/2SS-asd somatostatin DNA vaccine in postpartum lactating rats contributes to the growth of offspring, and improves the lactation performance of female rats indirectly. Weaning weight gain (215.8±18.8 g) of offspring of the middle dose intramuscular group was significantly higher than those of other groups, with a significant difference between the middle dose intramuscular group and the control group, and followed by the middle dose subcutaneous group (212.2±13.6 g). 3.Safety evaluation of somatostatin DNA vaccine in chromosomal integration.
At the end of immunization against pVGS/2SS-asd somatostatin DNA vaccine in rats with intramuscular or subcutaneous injections, multiple tissues such as heart, liver, spleen, lung, kidney, ovary and muscle were removed from each female rat of test groups, respectively. Moreover, tissue samples of the pups, such as heart, liver, spleen, lung and kidney were also collected after weaning, respectively. The genomic DNA was extracted, and then the genomic DNA samples were detected by sensitive PCR method. The results showed that the plasmid was not integrated into maternal and offspring genomic DNA, and the result revealed that the somatostatin DNA vaccine in chromosomal integration was safe to rats.
4. Effects of Somatostatin gene immunization on the reproduction and lactation performance of dairy cows.
The purpose of this study was to explore the effects of the new somatostatin DNA vaccine—pVGS/2SS-asd on the reproduction and lactation performance of postpartum dairy cows.40 postpartum cows were randomly divided into 4 groups,10 per group. At 7d after calving,3 test group cows were primarily intramuscular immunized against pVGS/2SS-asd in high (1×1011 CFU/1mL), the middle (1×1010CFU/1mL), and low (1×109 CFU/1mL) dose; the control group cows were intramuscular immunized against 10% aluminum hydroxide gel saline(1mL). Booster immunizations in the same dosage as the primary immunization (injection day as 0 d) were performed twice at 4-week intervals. The results showed that the novel pVGS/2SS-asd somatostatin DNA vaccine for cows had good immunogenicity and could induce a certain intensity of immune response. High dose group in the induction of the maximum antibody peak value (OD= 0.490±0.171) and the persistent high proportion of antibody positive cattle (100.0% for 7 w) had obvious advantages than other dose groups. After immunization, there was no significant difference among all the groups in the gravid uterine horn and uterine cervical involution, intervals of first ovulation and ovulation follicle diameter, this somatostatin DNA vaccine did not adversely affect the reproductive performance of dairy cows. Moreover it also confirmed that in the similar nutritional conditions, this vaccine improved the lactation performance of cows. The middle dose group had more advantages than other dose groups. Milk yields of the middle dose group were significantly higher than other groups for 5 weeks (2w to 6w) after primary immunization.
引文
1.白莉雅.pGM-CSF/SS基因免疫对雌鼠生长和繁殖的影响及其安全性研究.[硕士学位论文]. 武汉：华中农业大学图书馆,2008.
2.曹少先,杨利国,茆达干等.生长抑素基因疫苗pcS/SS构建及其在HeLa细胞中的表达.中国兽医学报,2004a,24：153-156.
3.曹少先.生长抑素DNA疫苗的构建及其对大鼠生长和相关激素的影响.[博士学位论文].南京：南京农业大学,2004b.
4.曹少先,张文伟,杨利国等.生长抑素基因疫苗质粒pcS/2SS的构建、表达及免疫.农业生物技术学报,2005a,13：477-481.
5.曹少先,杨利国,张文伟等.生长抑素基因疫苗质粒pEGS/2SS的构建及表达.中国兽医学报,2005b,25：499-502.
6.曹少先,杨利国,茆达干等.生长抑素DNA疫苗pEGS/2SS在大鼠体内的表达与分布.南京农业大学学报,2005c,28：137-139.
7.曹少先,何晓红,杨利国等.生长抑素基因疫苗pES/2SS的构建与表达.扬州大学学报(农业与生命科学版),2007,28：5-8.
8.陈汉荣,鲁成发,杨娅.医学影像技术丛书.超声显像分册.武汉：湖北科学技术出版社,2000,1-9
9.陈熠,贺建华.营养因素对奶牛繁殖性能的影响及提高繁殖率的措施.广东畜牧兽医科技,2009,34：21-23.
10.戴贤君,方维焕.减毒沙门氏菌为载体的草鱼口服生长抑素DNA疫苗的构建及其稳定性.中国兽医学报,2005a,25：356-358.
11.戴贤君,方维焕.减毒沙门氏菌为载体的口服生长抑素DNA疫苗对草鱼的安全性.中国兽医学报,2005b,25：466-469.
12.戴贤君.减毒沙门氏菌为载体的生长抑素DNA疫苗构建、表达及对草鱼生长影响研究.[博士学位论文].杭州：浙江大学,2005c.
13.何剑斌,田文儒.奶牛子宫复旧的研究方法.黑龙江畜牧兽医,1998,11：26-27
14.扈正甲,虎秉贤,张志强.产犊季节、胎次和产奶量对奶牛胎间距的影响.宁夏农林科技,2003,3：35-36；
15.黄健,郭建巍,蔡美英.辅助性T细胞表位预测.微生物学免疫学进展,2004,32：50-52.
16.黄维.染色体-质粒平衡致死系统的构建和应用。生物技术通讯,2002,13：378-382.
17.姜淑珍,扬在宾,闫庆兵等.奶牛微量元素锌营养研究进展.中国饲料,2004,2：25-27.
18.孔繁江,刘波,周传伟等.奶牛围产期注射维生素D3对生产和繁殖的影响.中国兽医杂志,1999,25：16.
19.李恭贺,何宝祥,张超.奶牛β-胡萝卜素营养及其缺乏症的防治.河北畜牧兽医,2003,19：38-39.
20.李静霞,王杨,汪连云.浅谈Se-VE在奶牛中的应用.江西畜牧兽医杂志,2004,1：24.
21.梁爱心,姚艳丰,杨利国等.小鼠对pGM-CSF/SS生长抑素基因疫苗的免疫应答.华中农业大学学报,2006,25：540-543.
22.刘大程,弓关.奶牛不孕症原因及防治措施.畜牧与饲料科学,2004,4：47-48.
23.刘建文,乐国伟,施用晖等.生长抑素pcDNA3s/SS质粒的构建与鉴定.无锡轻工大学学报,2002,21：187-190.
24.刘学剑.奶牛营养与繁殖疾病的关系.中国奶牛,1994,4：22-23.
25.舒邓群,茆达干,杨利国等.以减毒沙门氏菌为载体的GM-CSF与生长抑素融合表达质粒对小鼠淋巴细胞增殖和GH及IGF-Ⅰ分泌的影响.畜牧兽医学报,2006,37：814-818.
26.舒邓群,陈荣达,杨利国等.以减毒沙门氏菌为载体的GM-CSF与生长抑素DNA疫苗的安全性和稳定性.中国兽医学报,2007a,27：825-829.
27.舒邓群,茆达干,杨利国等.粒细胞-巨噬细胞集落刺激因子与生长抑素融合表达质粒对断奶仔猪生长及相关激素的影响.南京农业大学学报,2007b,30：92-96.
28.舒邓群,茆达干,杨利国等.粒细胞-巨噬细胞集落刺激因子基因佐剂对DNA疫苗的免疫调节作用.畜牧与兽医,2007c,39： 57-61.
29.舒邓群,茆达干,杨利国等.粒细胞-巨噬细胞集落刺激因子(GM-CSF)与生长抑素(SS)融合表达质粒的构建及其对小鼠的免疫效果.农业生物技术学报, 2008a,16：41-46.
30.舒邓群,茆达干,杨利国等.GM-CSF与SS融合表达质粒对小鼠淋巴细胞增殖及GH和IGF-1分泌的影响.扬州大学学报(农业与生命科学版),2008b,29：11-15.
31.孙树汉.影响核酸疫苗应用研究的若干问题.第二军医大学学报,2002,23：353-354.
32.孙迎基,汪兴生,张新元等.生长抑素DNA疫苗在COS细胞中的瞬时表达.药物生物技术,2002,9：71-73.
33.滕家波,苏国富.载体-宿主平衡致死系统在减毒伤寒沙门氏活菌苗中的应用.国外医学免疫学分册,1999,22：143-146.
34.田文儒,何剑斌,丛霞等.产后奶牛子宫内膜的扫描电镜观察.中国兽医学报,2004,24：183-186.
35.王新华,魏学良,李跃民等.奶牛产后子宫内膜复旧与腺体的形态计量研究.中国兽医科技,1997,27(1)：28-30.
36.汪腊根,欧阳建华,刘春生.微量元素、维生素对奶牛性能的影响.湖南饲料,2004,1：20-22.
37.薛春林,茆达干,杨利国等.生长抑素基因免疫对湖羊羔羊生长及GH和IGF-Ⅰ的影响.中国农业大学学报,2006,11：16-22.
38.薛春林,茆达干,杨利国等.基因佐剂CpGDNA对生长抑素DNA疫苗pES/2SS的作用.中国农业科学,2007,40：834-841.
39.杨利国,胡少昶,魏平华等.酶免疫测定技术.南京：南京大学出版社,1998,432-433.
40.杨利国主编.动物繁殖学.北京：中国农业出版社,2003,39-41,48-54,62-66,73-77.
41.杨章平,王杏龙,贝水荣等.黑白花奶牛产犊间隔及影响因素的分析.畜牧与兽医,1998,30：65-66.
42.张青萍,王新房. B型超声诊断学.上海：上海科学技术出版社,1992,13-17
43.周虚.营养对奶牛繁殖的影响.中国奶牛,2001,4：32-34.
44. Armstrong D G, McEvoy T G, Baxter G, et al. Effect of dietary energy and protein on bovine follicular dynamics and embryo production in vitro:Associations with the ovarian insulinlike growth factor system. Biol Reprod,2001,64:1624-1632.
45. Babiuk S, Baca-Estrada M E, Foldvari M, et al. Electroporation improves the efficacy of DNA vaccines in large animals. Vaccine,2002,20:3399-3408.
46. Babiuk S, van Drunen Littel-van den Hurk S, Babiuk L A. Delivery of DNA vaccines using electroporation. Methods Mol Med,2006,127:73-82.
47. Balogh O, Kovacs K, Kulcsar M, et al. Alul polymorphism of the bovine growth hormone (GH) gene, resumption of ovarian cyclicity, milk production and loss of body condition at the onset of lactation in dairy cows. Theriogenology,2009, 71:553-559.
48. Baruselli P S, Mucciolo R G, Vaina W G, et al. Postpartum uterine involution in buffalo cows (Bubalus bubalis). Bol Ind Anim,1996,53:51-56.
49. Bauer H, Darji A, Chakraborty T, et al. Salmonella-mediated oral DNA vaccination using stabilized eukaryotic expression plasmids. Gene Ther,2005,12:364-372.
50. Bauman D E and Vernon R G. Effects of exogenous bovine somatotropin on lactation. Annu Rev Nutr,1993,13:437-461.
51. Baumon D E, Epard P J, De Geeter M J. Response of high-producing dairy cows to longterm treatment with pituitary somatotropin and recombinant somatotropin. J Dairy Sci,1985,68:1352-1362.
52. Beam S W, Butler W R. Energy balance and ovarian follicle development prior to the first ovulation postpartum in dairy cows receiving three levels of dietary fat. Biol Reprod,1997,56:133-142.
53. Beam S W, Butler W R. Energy balance effects on follicular development and first ovulation in post-partum cows. J Repro. Fertil Suppl,1999,54:411-424
54. Bodles-Brakhop A M, Heller R, Draghia-Akli R. Electroporation for the delivery of DNA-based vaccines and immunotherapeutics:current clinical developments. Mol Ther,2009,17:585-592.
55. Bollo E, Biolatti B, Galloni M. Scanning electron microscopy of pathologic changesin the epithelial surface of unterus and uterine tubes of cow. Am J Vet Res, 1990,51(1):137-142.
56. Bona C, Radu D, Kodera T. Molecular studies on the diversification of hemagglutinin-specific human neonatal repertoire subsequent to immunization with naked DNA. Vaccine,2004,22:1624-1630
57. Bot A, Bot S, Garcia-Sastre A, et al. DNA immunization of newborn mice with a plasmid-expressing nucleoprotein of influenza virus. Viral Immunol,1996,9: 207-210.
58. Butler W R, Smith R D. Interrelationships between energy balance and postpartum reproductive function in dairy cattle. J Dairy Sci,1989,72:767-783.
59. Butler W R. Energy balance relationships with follicular development, ovulation and fertility in postpartum dairy cows. Livest Prod Sci,2003,83:211-221.
60. Butler W R. Nutritional interactions with reproductive performance in dairy cattle. Anim Reprod Sci,2000,60-61:449-457
61. Cardenas R, Lin X, Canosa L F, et al. Estradiol reduces pituitary responsiveness to somatostatin (SRIF-14) and down-regulates the expression of somatostatin sst2 receptors in female goldfish pituitary. Gen Comp Endocr,2003,132:119-124.
62. Chan R C, Gutierrez B, Ichim T E, et al. Enhancement of DNA cancer vaccine efficacy by combination with anti-angiogenesis in regression of established subcutaneous B16 melanoma. Oncol Rep,2009,22(5):1197-1203.
63. Chen A, Laskar-Levy O, Koch Y. Selective expression of neuropeptides in the rat mammary gland:Somatostatin gene is expressed during lactation. Endocrinology, 1999,140(12):5915-5921.
64. Colazo M G, Gordon M B, Rajamahendran R, et al. Pregnancy rates to timed artificial insemination in dairy cows treated with gonadotropin-releasing hormone or porcine luteinizing hormone. Theriogenology,2009,72:262-270.
65. Cui Z. DNA vaccine. Adv Genet,2005,54:257-289.
66. Darwash A O, Lamming G E, Woolliams J A. Estimation of genetic variation in the interval from calving to postpartum ovulation of dairy cows. J Dairy Sci,1997, 80:1227-1234.
67. Dimmick M A, Gimenez T, Spitzer J C. Ovarian endocrine activity and development of ovarian follicles during the postpartum interval in beef cows. Anim Reprod Sci, 1991,24:173-183.
68. Dobson H, Smith R, Royal M, et al. The high-producing dairy cow and its reproductive performance. Reprod Domest Anim,2007,42:17-23.
69. Elsaesser F, Drath S. The potential of immuno-neutralization of somatostatin for improving pig performance. Livest Prod Sci,1995,42:255-263.
70. Engin E, Stellbrink J, Treit D, et al. Anxiolytic and antidepressant effects of intracerebroventricularly administered somatostatin:behavioral and neurophysiological evidence. Neuroscience,2008,157(3):666-676.
71. Eo S K, Lee S, Chun S, et al. Modulation of immunity against herpes simplex virus infection via mucosal genetic transfer of plasmid DNA encoding chemokines. J Virol, 2001,75(2):569-578.
72. Farmer C, Brazeau P. Colostrum and milk composition of sows immunised against somatostatin and its carrier protein. Can J Anim Sci,1992,72:177-180.
73. Ferguson J D, Galligan D T, Thomsen N. Principal descriptors of body condition score in Holstein cows. J Dairy Sci,1994,77:2695-2703.
74. Flint D J, Tonner E, Beattie J, et al. Investigation of the mechanism of action of growth hormone in stimulating lactation in the rat. J Endcrinol,1992,134(3): 377-383.
75. Fourichon C, Seegers H, Malher X. Effects of disease on reproduction in the diary cow. A meta-analysis. Theriogenology,1999,53:1729-1759.
76. Galan J E, Nakayama K, Curtiss R 3rd. Cloning and characterization of the asd gene of Salmonella typhimurium:use in stable maintenance of recombinant plasmids in Salmonella vaccine strains. Gene,1990,94:29-35.
77. Garcia-Peniche T B, Cassell B G, Pearson R E, et al. Comparisons of Holsteins with Brown Swiss and Jersey Cows on the Same Farm for Age at First Calving and First Calving Interval. J Dairy Sci,2005,88:790-796.
78. Garmory H S, Brown KA, Titball R W. DNA vaccines:improving expression of antigens. Genet Vaccines Ther,2003, 1(1):2
79. Garnsworthy P C, Lock A, Mann G E, et al. Nutrition, metabolism, and fertility in dairy cows:1. Dietary energy source and ovarian function. J Dairy Sci,2008, 91:3814-3823.
80. Garssen G J, Welling A M A W, Spencer G S G. Active immunisation against somatostatin increases milk yield in goats. Neth JAgric Sci,1987,35:84-86.
81. Gilbert R 0, Shin S T, Guard C L, et al. Prevalence of endometritis and its effects on reproductive performance of dairy cows, Theriogenology,2005,64:1879-1888.
82. Gonzalez Sanchez J P, Bianchini Sobrinho E, Goncalves A A M. Uterine involution in dairy Gir cows, according to postpartum period and parity. Arq Bras Med Vet Zoo, 1999,51:345-351.
83. Gorewit R C, Oltenacu P A, Butler W R, et al. Effect of somatostatin (SRIF) administration on serum hormones, glucose and milk production in lactating cattle. J Dairy Sci,1977,60(Suppl.1):109. (Abstr.)
84. Grimard B, Humblot P, Ponter A A, et al. Influence of postpartum energy restriction on energy status, plasma LH and oestradiol secretion and follicular development in suckled beef cows. JReprod Fertil,1995,104:173-179.
85. Grohn Y T, Rajala-Schultz P. J. Epidemiology of reproductive performance in dairy cows. Anim Reprod Sci,2000,60-61:605-614.
86. Gumen A, Sartori R, Costa F M, et al. A GnRH/LH surge without subsequent progesterone exposure can induce development of follicular cysts. J Dairy Sci,2002, 85:43-50.
87. Gupta S, Gupta H K, Soni J. Effect of vitamin E and selenium supplementation on concentrations of plasma cortisol and erythrocyte lipid peroxides and the incidence of retained fetal membranes in crossbred dairy cattle. Theriogenology,2004, 64:1273-1286.
88. Hajurka J, Macak V, Hura V. Influence of health status of reproductive organs on uterine involution in dairy cows. B Vet Ⅰ Pulawy,2005,49:53-58.
89. Han L, Liang A X, Zhang J, et al. Evaluation of the VP22 gene adjuvant for enhancement of DNA vaccine against somatostatin in mice. Animal,2008,2: 1569-1574.
90. Han Y K, Kim I H. Risk factors for retained placenta and the effect of retained placenta on the occurrence of postpartum diseases and subsequent reproductive performance in dairy cows. J Vet Sci,2005,6:53-59.
91. Hatler T B, Hayes S H, Laranja da Fonesca L F, et al. Relationship between endogenous progesterone and follicular dynamics in lactating dairy cows with ovarian follicular cysts. Biol Reprod,2003,69:218-223.
92. Holloway A C, Sheridan M A, Leatherland J F. Estradiol inhibits plasma somatostatin 14 (SRIF-14) levels and inhibits the response of somatotrophic cells to SRIF-14 challenge in vitro in rainbow trout, Oncorhynchus mykiss. Gen Comp Endocr,1997, 106:407-414.
93. Horvath J E, Schally A V. Reciprocal changes in prolactin and growth hormone secretion in vitro after in vitro estrogen treatment. Acta Biologica Hungarica,1994, 45(2-4):249-262.
94. Houe H,Φstergaard S, Thilsing-Hansen T, et al. Milk fever and subclinical hypocalcaemia-an evaluation of parameters on incidence risk, diagnosis, risk factors and biological effects as input for a decision support system for disease control. Acta Veterinaria Scandinavica,2001,42:1-29.
95. Hu J, Cladel N, Balogh K, et al. Mucosally delivered peptides prime strong immunity in HLA-A2.1 transgenic rabbits. Vaccine,2010, Mar 20. [Epub ahead of print].
96. Hurley W L, Doane R M. Recent development in the roles of vitamins and mineral in production. J Dairy Sci,1989,72:784-804.
97. Ichino M, Mor G, Conover J, et al. Factors associated with the development of neonatal tolerance after the administration of a plasmid DNA vaccine. J Immunol, 1999,162:3814-3818.
98. Jechlinger W. Optimization and delivery of plasmid DNA for vaccination. Expert Rev Vaccines,2006,5:803-825.
99.Julien S, Laine J, Morisset J. Regulation of rat pancreatic CCKB receptor and somatostatin expression by insulin Diabetes,2004,53(6):1526-1534.
100. Kamimura S, Ohgi T, Takahashi M, et al. Postpartum resumption of ovarian activity and uterine involution monitored by ultrasonography in Holstein cows. J Vet Med Sci, 1993,55:643-647.
101. Kang H Y, Srinivasan J, Curtiss R 3rd. Immune responses to recombinant pneumococcal PspA antigen delivered by live attenuated Salmonella enterica serovar typhimurium vaccine. Infect Immun,2002,70:1739-1749.
102. Kim I H, Kang H G. Risk factors for postpartum endometritis and the effect of endometritis on reproductive performance in dairy cows in Korea. J Reprod Develop, 2003,49,485-491.
103. Kim J Y, Chung M I, Cho K K, et al. Effects of active immunization against somatostatin or somatostatin analogues on male rat growth. Food Agr Immunol,2001, 13(3):141-149.
104. Kirkwood R N, Korchinski R S, Thacker P A, et al. Observations on the influence of active immunization against somatostatin on the reproductive performance of sheep and pigs. JReprod Immunol,1990,17:229-238.
105. Koch Y, Werner H, Fridkin M. Hypothalamic hormones in milk. Endocr Regul, 1991,25(1-2):128-133.
106. Kohan-Ghadr H R, Lefebvre R C, Fecteau G, et al. Ultrasonographic and histological characterization of the placenta of somatic nuclear transfer-derived pregnancies in dairy cattle. Theriogenology,2008,69:218-230.
107. Krowczynska A M, Rudders R A, Krontiris T G. The human minisatellite consensus at breakpoints of oncogene translocations. Nucleic Acids Res,1990,18(5):1121-1127.
108. Kuhn M T, Hutchison J L, Wiggans G R. Characterization of Holstein Heifer Fertility in the United States. J Dairy Sci,2006,89:4907-4920.
109. Laven R A, Drew S B. Dietary protein and the reproductive performance of cows. Vet Rec,1999,11:687-695.
110. Laven R A, Peters A R. Bovine retained placenta:aetiology, pathogenesis and economic loss. Vet Rec,1996,139:465-471.
111. LeBlanc S J, Duffield T F, Leslie K E, et al. Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. J Dairy Sci,2002,85:2223-2236.
112. Ledwith B J, Manam S, Troilo P J, et al. Plasmid DNA vaccines:investigation of integration into host cellular DNA following intramuscular injection in mice. Intervirology,2000a,43:258-272.
113. Ledwith B J, Manam S, Troilo P J, et al. Plasmid DNA vaccines:assay for integration into host genomic DNA. Dev Biol,2000b,104:33-43.
114. Lee S C, Shin S H. Somatostatin does not inhibit prolactin synthesis in normal male rat pituitary cells but inhibits prolactin synthesis in estradiol-primed pituitary cells. J Endcrinol,1996,148(1):69-76.
115. Li G X, Qiu H J, Han C G, et al. Vaccination of plasmid DNA encoding somatostatin gene fused with GP5 gene of porcine reproductive and respiratory syndrome virus induces anti-GP5 antibodies and promotes growth performance in immunized pigs. Agr Sci China,2006,5:234-240.
116. Li G X, Zhou Y J, Yu H, et al. Prime-boost immunization with HA/C3d DNA followed by a recombinant pseudorabies virus boost enhanced protective immunity against H3N2 swine influenza virus in mice. Res Vet Sci,2010,88(2):345-351.
117. Liang A X, Cao S X, Han L, et al. Construction and evaluation of the eukaryotic expression plasmid encoding two copies of somatostatin genes fused with hepatitis B surface antigen gene S. Vaccine,2008,26:2935-2941.
118. Liang A X, Han L, Hua G H, et al. Construction of a fusion protein expression vector pGS/2SS-M4GFP without antibiotic resistance gene and its subcellular localization in different cell lines. Biologicals,2009,37:37-43.
119. Lin X W, Otto C J, Peter R E. Evolution of neuroendocrine peptide systems: Gonadotropin-releasing hormone and somatostatin. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol,1998; 119(3):375-388.
120. Liu D, Wang J, Niu Z X. Contribution of Chinese Pekin duck complement component C3d-P29 repeats to enhancement of Th2-biased immune responses against NDV F gene induced by DNA immunization. Immunopharmacol Immunotoxicol,2010, Feb 11. [Epub ahead of print]
121. Lucy M C. Reproductive loss in high-producing dairy cattle:where will it end? J Dairy Sci,2001,84:1277-1293.
122. Maizon D O, Oltenacu P A, Grohn Y T, et al. Effects of diseases on reproductive performance in Swedish red and white dairy cattle. Prev Vet Med,2004,66:113-126.
123. Manam S, Ledwith B J, Barnum A B, et al. Plasmid DNA vaccines:tissue distribution and effects of DNA sequence, adjuvants and delivery method on integration into host DNA. Intervirology,2000,43:273-281.
124. Mc Parland S, Kearney J F, Rath M, et al. Inbreeding effects on milk production, calving performance, fertility, and conformation in Irish Holstein-Friesians. J Dairy Sci,2007,90:4411-4419.
125. Mears G J. Immunization of lambs against somatostatin improve growth rate. Can J Anim Sci,1990; 70:1091-1097.
126. Medjitna T D, Stadler C, Bruckner L, et al. DNA vaccines:safety aspect assessment and regulation. Dev Biol,2006,126:261-270.
127. Mee J F, Buckley F. Management, nutrition and breeding strategies to improve dairy herd fertility. In:Proceedings of the Teagasc Dairy Conference.2003.
128. Miettinen P V. Uterine involution in Finnish dairy cows. Acta Vet Scand,1990, 31:181-185.
129. Milosevic V, Nestorovic N, Filipovic B, et al. Centrally applied somatostatin influences morphology of pituitary FSH cells but not FSH release. Gen Physiol Biophys,2004,23:375-380.
130. Montaserti A, Pourheydar M, Khazaei M, et al. Anti-fertility effects of physalis alkekengi alcoholic extract in female rat. Iran J Reprod Med,2007,5:13-16.
131. Mor G, Yamshchikov G, Sedegah M, et al. Induction of neonatal tolerance by plasmid DNA vaccination of mice. J Clin Invest,1996,98:2700-2705.
132. Movsesyan N, Mkrtichyan M, Petrushina I, et al. DNA epitope vaccine containing complement component C3d enhances anti-amyloid-beta antibody production and polarizes the immune response towards a Th2 phenotype. J Neuroimmunol,2008, 205:57-63.
133. Myung K H. Effect of recombinant bovine somatotropin on milk production and milk composition in dairy cows. Asian Austral JAnim,1990,3:247-252.
134. Nakayama K, Kelly S M, Curtiss R 3rd. Construction of an asd+ expression-cloning vector:stable maintenance and high level expression of cloned genes in a Salmonella vaccine strain. Biotechnology,1988,6:693-697.
135. National Research Council. Nutrient Requirements of Dairy Cattle.7th rev. ed. Natl. Acad. Sci., Washington, DC,2001.
136. Nogueira L A G, Pinheiro L E L, Norte A L, et al. Uterine involution and return to cycling ovarian activity in zebu cows. Revista Brasileira De Reproducao Anima, 1993,17:49-56.
137. Norman H D, Wright J R, Hubbard S M, et al. Reproductive status of Holstein and Jersey cows in the United States. J Dairy Sci,2009,92:3517-3528.
138. Okano A, Tomizuka T. Ultrasonic observation of postpartum uterine involution in the cow. Theriogenology,1987,27:369-376.
139. Pillon D, Caraty A, Fabre-Nys C, et al. Regulation by estradiol of hypothalamic somatostatin gene expression:possible involvement of somatostatin in the control of luteinizing hormone secretion in the ewe. Biol Reprod,2004,71:38-44.
140. Pizzi F, Rizzi R, Maltecca C, et al. Fertility and longevity in the Reggiana cattle breed. Ital J Anim Sci,2003,2(suppl.1):151-153.
141. Pryce J E, Royal M D, Garnsworthy P C, et al. Fertility in the high-producing dairy cow. Livest Prod Sci,2004,86:125-135.
142. Pryce J E, Veerkamp R F. The incorporation of fertility indices in genetic improvement programmes. Occ Publ Br Soc Anim Sci,2001,26:237-250.
143. Ralph M W. Biology of the uterus.2nd ed. New York:Plenum Press,1977.
144. Rao A V N, Babu M S, Rao H R M. Evaluation of fertility traits in Jersey cows. Indian Vet J,1995,72:939-942.
145. Reisine T. Somatostatin. Cell Mol Neurobiol,1995; 15(6):597-614.
146. Rekwot P I, Ogwu D, Oyedipe E O. Influence of bull biostimulation, season and parity on resumption of ovarian activity of zebu (Bos indicus) cattle following parturition. Anim Reprod Sci,2000,63:1-11.
147. Ren SG, Kim S, Taylor J, et al. Suppression of rat and human growth hormone and prolactin secretion by a novel somatostatin/dopaminergic chimeric ligand. J Clin Endocrinol Metab,2003,88(11):5414-5421
148. Robertson J S. Safety considerations for nucleic acid vaccines. Vaccine,1994,12: 1526-1528.
149. Roche J F. The effect of nutritional management of the dairy cow on reproductive efficiency. Anim Reprod Sci,2006,96:282-296.
150. Romero J J, Canas R, Baldwin R L. A technique for estimating milk production in rats. JNutr,1975,105:413-420.
151. Royal M D, Darwash A O, Flint A P F, et al. Declining fertility in dairy cattle: changes in traditional and endocrine parameters of fertility. Anim Sci,2000,70: 487-501.
152. Riidiger N S, Gregersen N, Kielland-Brandt M C. One shortwell conserved region of Alu-sequences is involved in human gene rearrangements and has homology with prokaryotic chi. Nucleic Acids Res,1995,23:256-260.
153. Sa Filho M F, Ayres H, Ferreira R M, et al. Equine chorionic gonadotropin and gonadotropin-releasing hormone enhance fertility in a norgestomet-based, timed artificial insemination protocol in suckled Nelore (Bos indicus) cows. Theriogenology,2010,73:651-658.
154. Sampson D A and Jansen G R, Measurement of milk yield in the lactating rat from pup weight and weight gain. J Pediatr Gastroenterol Nutr,1984,3:613-617.
155. Samuelsson B, Uvnas-Moberg K, Gorewit R C, et al. Profiles of the hormones somatostatin, gastrin, CCK, prolactin, growth hormone and cortisol. I. In dairy cows that are milked and fed separately or milked and fed simultaneously. Livest Prod Sci, 1996,46:49-56.
156. Savio JD, Boland M P, Roche J F. Development of dominant follicles and length of ovarian cycles in postpartum dairy cows. J Reprod Fertil,1990,88:581-591.
157. Scrable H, Stambrook P J. A genetic program for deletion of foreign DNA from the mammalian genome. Mutat Res,1999,429(2):225-237.
158. Shata M T, Stevceva L, Agwale S, et al. Recent advances with recombinant bacterial vaccine vectors. Molecular Medicine Today,2000,6:66-71.
159. Sheldon I M, Dobson H. Effect of administration of eCG to postpartum cows on folliculogenesis in the ovary ipsilateral to the previously gravid uterine horn and uterine involution. J Reprod Fertil,2000,119:157-163.
160. Sheldon I M, Dobson H. Postpartum uterine health in cattle. Anim Reprod Sci,2004, 82-83:295-306.
161. Sheldon I M. The postpartum uterus. Vet Clin NAm-Food A,2004,20:569-591.
162. Shrestha H K, Nakao T, Suzuki T, et al. Relationships between body condition score, body weight, and some nutritional parameters in plasma and resumption of ovarian cyclicity postpartum during pre-service period in high-producing dairy cows in a subtropical region in Japan. Theriogenology,2005,64:855-66.
163. Simpson R B, Chase C C Jr, Spicer L J, et al. Effect of exogenous insulin on plasma and follicular insulin-like growth factor Ⅰ, insulin-like growth factor bindingprotein activity, follicular oestradiol and progesterone, and follicular growth in superovulated Angus and Brahman cows. J Reprod Fertil,1994,102:483-492.
164. Sinclair K D, Kuran M, Gebbie F E, et al. Nitrogen metabolism and fertility in cattle: Ⅱ. Development of oocytes recovered from heifers offered diets differing in their rate of nitrogen release in the rumen. J Anim Sci,2000,78:2670-2680.
165. Spicer L J, Alpizar E, Echternkamp S E. Effects of insulin, insulin-like growth factor I, and gonadotropins on bovine granulose cell proliferation, progesterone production, estradiol production and or insulin-like growth factor-I production. J Anim Sci,1993,71:1232-1241.
166. Sun S S, Jang K, Kuk K, et al. The effects of somatostatin passive immunization on milk yield, plasma hormone and metabolite concentrations in rats. Asian Austral J Anim,1997,10:324-328.
167. Sun S S, Myung K H, Spencer G S G. Influence of endogenous somatostatin on lactation in rats. Asian Austral J Anim,1996,9(3):337-340.
168. Tanaka T, Arai M, Ohtani S, et al. Influence of parity on follicular dynamics and resumption of ovarian cycle in postpartum dairy cows. Anim Reprod Sci,2008, 108:134-143.
169. Temin H M. Overview of biological effects of addition of DNA moleculars to cells. J Med Virol,1990,31:13-17.
170. Ulmer J B, Wahren B, Liu M A. DNA vaccines:recent technological and clinical advances. Discov Med,2006,6(33):109-112
171. Van Vugt H H, Swarts H J, Van de Heijning B J, et al. Centrally applied somatostatin inhibits the estrogen-induced luteinizing hormone surge via hypothalamic gonadotropin-releasing hormone cell activation in female rats. Biol Reprod,2004,71:813-819.
172. Vanholder T, Leroy J L, Dewulf J, et al. Hormonal and metabolic profiles of high-yielding dairy cows prior to ovarian cyst formation or first ovulation post partum. Reprod Domest Anim,2005,40:460-467.
173. Wagner W C, Hansel W. Reproductive physiology of the post partum cow. I. Clinical and histological findings. J Reprod Fertil,1969,18(3):493-500.
174. Waterston A M, Gumbrell L, Bratt T, et al. Phase I study of TNF-a Auto VaccIne in Patients with metastatic cancer. Cancer Immunol Immunother,2005,54:848-857
175. Westwood C T, Lean I J, Garavin J K, et al. Effects of genetic merit and varying dietary protein degradability on lactating dairy cows. J Dairy Sci,2000, 83:2926-2940.
176. Xue C L, Mao D G, Yang L G, et al. Effect of gene adjuvant CpGDNA on the somatostatin DNA vaccine in mice. Scientia Agricultura Sinica,2007; 40(4):834-41.
177. Yi I, Lim D, Kim J, et al. Effects of active immunization against somatostatin or somatostatin analogues on milk production in rats. Nutr Res,1999,19(7):1061-1072.
178. Zain A E, Nakao T, Raouf M A, et al. Factors in the resumption of ovarian activity and uterine involution in postpartum dairy cows. Anim Reprod Sci,1995,38: 203-214.
179. Zulu V C, Sawamukai Y, Nakada K, et al. Relationship among insulin-like growth factor-Ⅰ, blood metabolites and postpartum ovarian function in dairy cows. J Vet Med Sci, 2002,64:879-885.
2.曹少先,杨利国,茆达干等.生长抑素基因疫苗pcS/SS构建及其在HeLa细胞中的表达.中国兽医学报,2004a,24：153-156.
3.曹少先.生长抑素DNA疫苗的构建及其对大鼠生长和相关激素的影响.[博士学位论文].南京：南京农业大学,2004b.
4.曹少先,张文伟,杨利国等.生长抑素基因疫苗质粒pcS/2SS的构建、表达及免疫.农业生物技术学报,2005a,13：477-481.
5.曹少先,杨利国,张文伟等.生长抑素基因疫苗质粒pEGS/2SS的构建及表达.中国兽医学报,2005b,25：499-502.
6.曹少先,杨利国,茆达干等.生长抑素DNA疫苗pEGS/2SS在大鼠体内的表达与分布.南京农业大学学报,2005c,28：137-139.
7.曹少先,何晓红,杨利国等.生长抑素基因疫苗pES/2SS的构建与表达.扬州大学学报(农业与生命科学版),2007,28：5-8.
8.陈汉荣,鲁成发,杨娅.医学影像技术丛书.超声显像分册.武汉：湖北科学技术出版社,2000,1-9
9.陈熠,贺建华.营养因素对奶牛繁殖性能的影响及提高繁殖率的措施.广东畜牧兽医科技,2009,34：21-23.
10.戴贤君,方维焕.减毒沙门氏菌为载体的草鱼口服生长抑素DNA疫苗的构建及其稳定性.中国兽医学报,2005a,25：356-358.
11.戴贤君,方维焕.减毒沙门氏菌为载体的口服生长抑素DNA疫苗对草鱼的安全性.中国兽医学报,2005b,25：466-469.
12.戴贤君.减毒沙门氏菌为载体的生长抑素DNA疫苗构建、表达及对草鱼生长影响研究.[博士学位论文].杭州：浙江大学,2005c.
13.何剑斌,田文儒.奶牛子宫复旧的研究方法.黑龙江畜牧兽医,1998,11：26-27
14.扈正甲,虎秉贤,张志强.产犊季节、胎次和产奶量对奶牛胎间距的影响.宁夏农林科技,2003,3：35-36；
15.黄健,郭建巍,蔡美英.辅助性T细胞表位预测.微生物学免疫学进展,2004,32：50-52.
16.黄维.染色体-质粒平衡致死系统的构建和应用。生物技术通讯,2002,13：378-382.
17.姜淑珍,扬在宾,闫庆兵等.奶牛微量元素锌营养研究进展.中国饲料,2004,2：25-27.
18.孔繁江,刘波,周传伟等.奶牛围产期注射维生素D3对生产和繁殖的影响.中国兽医杂志,1999,25：16.
19.李恭贺,何宝祥,张超.奶牛β-胡萝卜素营养及其缺乏症的防治.河北畜牧兽医,2003,19：38-39.
20.李静霞,王杨,汪连云.浅谈Se-VE在奶牛中的应用.江西畜牧兽医杂志,2004,1：24.
21.梁爱心,姚艳丰,杨利国等.小鼠对pGM-CSF/SS生长抑素基因疫苗的免疫应答.华中农业大学学报,2006,25：540-543.
22.刘大程,弓关.奶牛不孕症原因及防治措施.畜牧与饲料科学,2004,4：47-48.
23.刘建文,乐国伟,施用晖等.生长抑素pcDNA3s/SS质粒的构建与鉴定.无锡轻工大学学报,2002,21：187-190.
24.刘学剑.奶牛营养与繁殖疾病的关系.中国奶牛,1994,4：22-23.
25.舒邓群,茆达干,杨利国等.以减毒沙门氏菌为载体的GM-CSF与生长抑素融合表达质粒对小鼠淋巴细胞增殖和GH及IGF-Ⅰ分泌的影响.畜牧兽医学报,2006,37：814-818.
26.舒邓群,陈荣达,杨利国等.以减毒沙门氏菌为载体的GM-CSF与生长抑素DNA疫苗的安全性和稳定性.中国兽医学报,2007a,27：825-829.
27.舒邓群,茆达干,杨利国等.粒细胞-巨噬细胞集落刺激因子与生长抑素融合表达质粒对断奶仔猪生长及相关激素的影响.南京农业大学学报,2007b,30：92-96.
28.舒邓群,茆达干,杨利国等.粒细胞-巨噬细胞集落刺激因子基因佐剂对DNA疫苗的免疫调节作用.畜牧与兽医,2007c,39： 57-61.
29.舒邓群,茆达干,杨利国等.粒细胞-巨噬细胞集落刺激因子(GM-CSF)与生长抑素(SS)融合表达质粒的构建及其对小鼠的免疫效果.农业生物技术学报, 2008a,16：41-46.
30.舒邓群,茆达干,杨利国等.GM-CSF与SS融合表达质粒对小鼠淋巴细胞增殖及GH和IGF-1分泌的影响.扬州大学学报(农业与生命科学版),2008b,29：11-15.
31.孙树汉.影响核酸疫苗应用研究的若干问题.第二军医大学学报,2002,23：353-354.
32.孙迎基,汪兴生,张新元等.生长抑素DNA疫苗在COS细胞中的瞬时表达.药物生物技术,2002,9：71-73.
33.滕家波,苏国富.载体-宿主平衡致死系统在减毒伤寒沙门氏活菌苗中的应用.国外医学免疫学分册,1999,22：143-146.
34.田文儒,何剑斌,丛霞等.产后奶牛子宫内膜的扫描电镜观察.中国兽医学报,2004,24：183-186.
35.王新华,魏学良,李跃民等.奶牛产后子宫内膜复旧与腺体的形态计量研究.中国兽医科技,1997,27(1)：28-30.
36.汪腊根,欧阳建华,刘春生.微量元素、维生素对奶牛性能的影响.湖南饲料,2004,1：20-22.
37.薛春林,茆达干,杨利国等.生长抑素基因免疫对湖羊羔羊生长及GH和IGF-Ⅰ的影响.中国农业大学学报,2006,11：16-22.
38.薛春林,茆达干,杨利国等.基因佐剂CpGDNA对生长抑素DNA疫苗pES/2SS的作用.中国农业科学,2007,40：834-841.
39.杨利国,胡少昶,魏平华等.酶免疫测定技术.南京：南京大学出版社,1998,432-433.
40.杨利国主编.动物繁殖学.北京：中国农业出版社,2003,39-41,48-54,62-66,73-77.
41.杨章平,王杏龙,贝水荣等.黑白花奶牛产犊间隔及影响因素的分析.畜牧与兽医,1998,30：65-66.
42.张青萍,王新房. B型超声诊断学.上海：上海科学技术出版社,1992,13-17
43.周虚.营养对奶牛繁殖的影响.中国奶牛,2001,4：32-34.
44. Armstrong D G, McEvoy T G, Baxter G, et al. Effect of dietary energy and protein on bovine follicular dynamics and embryo production in vitro:Associations with the ovarian insulinlike growth factor system. Biol Reprod,2001,64:1624-1632.
45. Babiuk S, Baca-Estrada M E, Foldvari M, et al. Electroporation improves the efficacy of DNA vaccines in large animals. Vaccine,2002,20:3399-3408.
46. Babiuk S, van Drunen Littel-van den Hurk S, Babiuk L A. Delivery of DNA vaccines using electroporation. Methods Mol Med,2006,127:73-82.
47. Balogh O, Kovacs K, Kulcsar M, et al. Alul polymorphism of the bovine growth hormone (GH) gene, resumption of ovarian cyclicity, milk production and loss of body condition at the onset of lactation in dairy cows. Theriogenology,2009, 71:553-559.
48. Baruselli P S, Mucciolo R G, Vaina W G, et al. Postpartum uterine involution in buffalo cows (Bubalus bubalis). Bol Ind Anim,1996,53:51-56.
49. Bauer H, Darji A, Chakraborty T, et al. Salmonella-mediated oral DNA vaccination using stabilized eukaryotic expression plasmids. Gene Ther,2005,12:364-372.
50. Bauman D E and Vernon R G. Effects of exogenous bovine somatotropin on lactation. Annu Rev Nutr,1993,13:437-461.
51. Baumon D E, Epard P J, De Geeter M J. Response of high-producing dairy cows to longterm treatment with pituitary somatotropin and recombinant somatotropin. J Dairy Sci,1985,68:1352-1362.
52. Beam S W, Butler W R. Energy balance and ovarian follicle development prior to the first ovulation postpartum in dairy cows receiving three levels of dietary fat. Biol Reprod,1997,56:133-142.
53. Beam S W, Butler W R. Energy balance effects on follicular development and first ovulation in post-partum cows. J Repro. Fertil Suppl,1999,54:411-424
54. Bodles-Brakhop A M, Heller R, Draghia-Akli R. Electroporation for the delivery of DNA-based vaccines and immunotherapeutics:current clinical developments. Mol Ther,2009,17:585-592.
55. Bollo E, Biolatti B, Galloni M. Scanning electron microscopy of pathologic changesin the epithelial surface of unterus and uterine tubes of cow. Am J Vet Res, 1990,51(1):137-142.
56. Bona C, Radu D, Kodera T. Molecular studies on the diversification of hemagglutinin-specific human neonatal repertoire subsequent to immunization with naked DNA. Vaccine,2004,22:1624-1630
57. Bot A, Bot S, Garcia-Sastre A, et al. DNA immunization of newborn mice with a plasmid-expressing nucleoprotein of influenza virus. Viral Immunol,1996,9: 207-210.
58. Butler W R, Smith R D. Interrelationships between energy balance and postpartum reproductive function in dairy cattle. J Dairy Sci,1989,72:767-783.
59. Butler W R. Energy balance relationships with follicular development, ovulation and fertility in postpartum dairy cows. Livest Prod Sci,2003,83:211-221.
60. Butler W R. Nutritional interactions with reproductive performance in dairy cattle. Anim Reprod Sci,2000,60-61:449-457
61. Cardenas R, Lin X, Canosa L F, et al. Estradiol reduces pituitary responsiveness to somatostatin (SRIF-14) and down-regulates the expression of somatostatin sst2 receptors in female goldfish pituitary. Gen Comp Endocr,2003,132:119-124.
62. Chan R C, Gutierrez B, Ichim T E, et al. Enhancement of DNA cancer vaccine efficacy by combination with anti-angiogenesis in regression of established subcutaneous B16 melanoma. Oncol Rep,2009,22(5):1197-1203.
63. Chen A, Laskar-Levy O, Koch Y. Selective expression of neuropeptides in the rat mammary gland:Somatostatin gene is expressed during lactation. Endocrinology, 1999,140(12):5915-5921.
64. Colazo M G, Gordon M B, Rajamahendran R, et al. Pregnancy rates to timed artificial insemination in dairy cows treated with gonadotropin-releasing hormone or porcine luteinizing hormone. Theriogenology,2009,72:262-270.
65. Cui Z. DNA vaccine. Adv Genet,2005,54:257-289.
66. Darwash A O, Lamming G E, Woolliams J A. Estimation of genetic variation in the interval from calving to postpartum ovulation of dairy cows. J Dairy Sci,1997, 80:1227-1234.
67. Dimmick M A, Gimenez T, Spitzer J C. Ovarian endocrine activity and development of ovarian follicles during the postpartum interval in beef cows. Anim Reprod Sci, 1991,24:173-183.
68. Dobson H, Smith R, Royal M, et al. The high-producing dairy cow and its reproductive performance. Reprod Domest Anim,2007,42:17-23.
69. Elsaesser F, Drath S. The potential of immuno-neutralization of somatostatin for improving pig performance. Livest Prod Sci,1995,42:255-263.
70. Engin E, Stellbrink J, Treit D, et al. Anxiolytic and antidepressant effects of intracerebroventricularly administered somatostatin:behavioral and neurophysiological evidence. Neuroscience,2008,157(3):666-676.
71. Eo S K, Lee S, Chun S, et al. Modulation of immunity against herpes simplex virus infection via mucosal genetic transfer of plasmid DNA encoding chemokines. J Virol, 2001,75(2):569-578.
72. Farmer C, Brazeau P. Colostrum and milk composition of sows immunised against somatostatin and its carrier protein. Can J Anim Sci,1992,72:177-180.
73. Ferguson J D, Galligan D T, Thomsen N. Principal descriptors of body condition score in Holstein cows. J Dairy Sci,1994,77:2695-2703.
74. Flint D J, Tonner E, Beattie J, et al. Investigation of the mechanism of action of growth hormone in stimulating lactation in the rat. J Endcrinol,1992,134(3): 377-383.
75. Fourichon C, Seegers H, Malher X. Effects of disease on reproduction in the diary cow. A meta-analysis. Theriogenology,1999,53:1729-1759.
76. Galan J E, Nakayama K, Curtiss R 3rd. Cloning and characterization of the asd gene of Salmonella typhimurium:use in stable maintenance of recombinant plasmids in Salmonella vaccine strains. Gene,1990,94:29-35.
77. Garcia-Peniche T B, Cassell B G, Pearson R E, et al. Comparisons of Holsteins with Brown Swiss and Jersey Cows on the Same Farm for Age at First Calving and First Calving Interval. J Dairy Sci,2005,88:790-796.
78. Garmory H S, Brown KA, Titball R W. DNA vaccines:improving expression of antigens. Genet Vaccines Ther,2003, 1(1):2
79. Garnsworthy P C, Lock A, Mann G E, et al. Nutrition, metabolism, and fertility in dairy cows:1. Dietary energy source and ovarian function. J Dairy Sci,2008, 91:3814-3823.
80. Garssen G J, Welling A M A W, Spencer G S G. Active immunisation against somatostatin increases milk yield in goats. Neth JAgric Sci,1987,35:84-86.
81. Gilbert R 0, Shin S T, Guard C L, et al. Prevalence of endometritis and its effects on reproductive performance of dairy cows, Theriogenology,2005,64:1879-1888.
82. Gonzalez Sanchez J P, Bianchini Sobrinho E, Goncalves A A M. Uterine involution in dairy Gir cows, according to postpartum period and parity. Arq Bras Med Vet Zoo, 1999,51:345-351.
83. Gorewit R C, Oltenacu P A, Butler W R, et al. Effect of somatostatin (SRIF) administration on serum hormones, glucose and milk production in lactating cattle. J Dairy Sci,1977,60(Suppl.1):109. (Abstr.)
84. Grimard B, Humblot P, Ponter A A, et al. Influence of postpartum energy restriction on energy status, plasma LH and oestradiol secretion and follicular development in suckled beef cows. JReprod Fertil,1995,104:173-179.
85. Grohn Y T, Rajala-Schultz P. J. Epidemiology of reproductive performance in dairy cows. Anim Reprod Sci,2000,60-61:605-614.
86. Gumen A, Sartori R, Costa F M, et al. A GnRH/LH surge without subsequent progesterone exposure can induce development of follicular cysts. J Dairy Sci,2002, 85:43-50.
87. Gupta S, Gupta H K, Soni J. Effect of vitamin E and selenium supplementation on concentrations of plasma cortisol and erythrocyte lipid peroxides and the incidence of retained fetal membranes in crossbred dairy cattle. Theriogenology,2004, 64:1273-1286.
88. Hajurka J, Macak V, Hura V. Influence of health status of reproductive organs on uterine involution in dairy cows. B Vet Ⅰ Pulawy,2005,49:53-58.
89. Han L, Liang A X, Zhang J, et al. Evaluation of the VP22 gene adjuvant for enhancement of DNA vaccine against somatostatin in mice. Animal,2008,2: 1569-1574.
90. Han Y K, Kim I H. Risk factors for retained placenta and the effect of retained placenta on the occurrence of postpartum diseases and subsequent reproductive performance in dairy cows. J Vet Sci,2005,6:53-59.
91. Hatler T B, Hayes S H, Laranja da Fonesca L F, et al. Relationship between endogenous progesterone and follicular dynamics in lactating dairy cows with ovarian follicular cysts. Biol Reprod,2003,69:218-223.
92. Holloway A C, Sheridan M A, Leatherland J F. Estradiol inhibits plasma somatostatin 14 (SRIF-14) levels and inhibits the response of somatotrophic cells to SRIF-14 challenge in vitro in rainbow trout, Oncorhynchus mykiss. Gen Comp Endocr,1997, 106:407-414.
93. Horvath J E, Schally A V. Reciprocal changes in prolactin and growth hormone secretion in vitro after in vitro estrogen treatment. Acta Biologica Hungarica,1994, 45(2-4):249-262.
94. Houe H,Φstergaard S, Thilsing-Hansen T, et al. Milk fever and subclinical hypocalcaemia-an evaluation of parameters on incidence risk, diagnosis, risk factors and biological effects as input for a decision support system for disease control. Acta Veterinaria Scandinavica,2001,42:1-29.
95. Hu J, Cladel N, Balogh K, et al. Mucosally delivered peptides prime strong immunity in HLA-A2.1 transgenic rabbits. Vaccine,2010, Mar 20. [Epub ahead of print].
96. Hurley W L, Doane R M. Recent development in the roles of vitamins and mineral in production. J Dairy Sci,1989,72:784-804.
97. Ichino M, Mor G, Conover J, et al. Factors associated with the development of neonatal tolerance after the administration of a plasmid DNA vaccine. J Immunol, 1999,162:3814-3818.
98. Jechlinger W. Optimization and delivery of plasmid DNA for vaccination. Expert Rev Vaccines,2006,5:803-825.
99.Julien S, Laine J, Morisset J. Regulation of rat pancreatic CCKB receptor and somatostatin expression by insulin Diabetes,2004,53(6):1526-1534.
100. Kamimura S, Ohgi T, Takahashi M, et al. Postpartum resumption of ovarian activity and uterine involution monitored by ultrasonography in Holstein cows. J Vet Med Sci, 1993,55:643-647.
101. Kang H Y, Srinivasan J, Curtiss R 3rd. Immune responses to recombinant pneumococcal PspA antigen delivered by live attenuated Salmonella enterica serovar typhimurium vaccine. Infect Immun,2002,70:1739-1749.
102. Kim I H, Kang H G. Risk factors for postpartum endometritis and the effect of endometritis on reproductive performance in dairy cows in Korea. J Reprod Develop, 2003,49,485-491.
103. Kim J Y, Chung M I, Cho K K, et al. Effects of active immunization against somatostatin or somatostatin analogues on male rat growth. Food Agr Immunol,2001, 13(3):141-149.
104. Kirkwood R N, Korchinski R S, Thacker P A, et al. Observations on the influence of active immunization against somatostatin on the reproductive performance of sheep and pigs. JReprod Immunol,1990,17:229-238.
105. Koch Y, Werner H, Fridkin M. Hypothalamic hormones in milk. Endocr Regul, 1991,25(1-2):128-133.
106. Kohan-Ghadr H R, Lefebvre R C, Fecteau G, et al. Ultrasonographic and histological characterization of the placenta of somatic nuclear transfer-derived pregnancies in dairy cattle. Theriogenology,2008,69:218-230.
107. Krowczynska A M, Rudders R A, Krontiris T G. The human minisatellite consensus at breakpoints of oncogene translocations. Nucleic Acids Res,1990,18(5):1121-1127.
108. Kuhn M T, Hutchison J L, Wiggans G R. Characterization of Holstein Heifer Fertility in the United States. J Dairy Sci,2006,89:4907-4920.
109. Laven R A, Drew S B. Dietary protein and the reproductive performance of cows. Vet Rec,1999,11:687-695.
110. Laven R A, Peters A R. Bovine retained placenta:aetiology, pathogenesis and economic loss. Vet Rec,1996,139:465-471.
111. LeBlanc S J, Duffield T F, Leslie K E, et al. Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. J Dairy Sci,2002,85:2223-2236.
112. Ledwith B J, Manam S, Troilo P J, et al. Plasmid DNA vaccines:investigation of integration into host cellular DNA following intramuscular injection in mice. Intervirology,2000a,43:258-272.
113. Ledwith B J, Manam S, Troilo P J, et al. Plasmid DNA vaccines:assay for integration into host genomic DNA. Dev Biol,2000b,104:33-43.
114. Lee S C, Shin S H. Somatostatin does not inhibit prolactin synthesis in normal male rat pituitary cells but inhibits prolactin synthesis in estradiol-primed pituitary cells. J Endcrinol,1996,148(1):69-76.
115. Li G X, Qiu H J, Han C G, et al. Vaccination of plasmid DNA encoding somatostatin gene fused with GP5 gene of porcine reproductive and respiratory syndrome virus induces anti-GP5 antibodies and promotes growth performance in immunized pigs. Agr Sci China,2006,5:234-240.
116. Li G X, Zhou Y J, Yu H, et al. Prime-boost immunization with HA/C3d DNA followed by a recombinant pseudorabies virus boost enhanced protective immunity against H3N2 swine influenza virus in mice. Res Vet Sci,2010,88(2):345-351.
117. Liang A X, Cao S X, Han L, et al. Construction and evaluation of the eukaryotic expression plasmid encoding two copies of somatostatin genes fused with hepatitis B surface antigen gene S. Vaccine,2008,26:2935-2941.
118. Liang A X, Han L, Hua G H, et al. Construction of a fusion protein expression vector pGS/2SS-M4GFP without antibiotic resistance gene and its subcellular localization in different cell lines. Biologicals,2009,37:37-43.
119. Lin X W, Otto C J, Peter R E. Evolution of neuroendocrine peptide systems: Gonadotropin-releasing hormone and somatostatin. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol,1998; 119(3):375-388.
120. Liu D, Wang J, Niu Z X. Contribution of Chinese Pekin duck complement component C3d-P29 repeats to enhancement of Th2-biased immune responses against NDV F gene induced by DNA immunization. Immunopharmacol Immunotoxicol,2010, Feb 11. [Epub ahead of print]
121. Lucy M C. Reproductive loss in high-producing dairy cattle:where will it end? J Dairy Sci,2001,84:1277-1293.
122. Maizon D O, Oltenacu P A, Grohn Y T, et al. Effects of diseases on reproductive performance in Swedish red and white dairy cattle. Prev Vet Med,2004,66:113-126.
123. Manam S, Ledwith B J, Barnum A B, et al. Plasmid DNA vaccines:tissue distribution and effects of DNA sequence, adjuvants and delivery method on integration into host DNA. Intervirology,2000,43:273-281.
124. Mc Parland S, Kearney J F, Rath M, et al. Inbreeding effects on milk production, calving performance, fertility, and conformation in Irish Holstein-Friesians. J Dairy Sci,2007,90:4411-4419.
125. Mears G J. Immunization of lambs against somatostatin improve growth rate. Can J Anim Sci,1990; 70:1091-1097.
126. Medjitna T D, Stadler C, Bruckner L, et al. DNA vaccines:safety aspect assessment and regulation. Dev Biol,2006,126:261-270.
127. Mee J F, Buckley F. Management, nutrition and breeding strategies to improve dairy herd fertility. In:Proceedings of the Teagasc Dairy Conference.2003.
128. Miettinen P V. Uterine involution in Finnish dairy cows. Acta Vet Scand,1990, 31:181-185.
129. Milosevic V, Nestorovic N, Filipovic B, et al. Centrally applied somatostatin influences morphology of pituitary FSH cells but not FSH release. Gen Physiol Biophys,2004,23:375-380.
130. Montaserti A, Pourheydar M, Khazaei M, et al. Anti-fertility effects of physalis alkekengi alcoholic extract in female rat. Iran J Reprod Med,2007,5:13-16.
131. Mor G, Yamshchikov G, Sedegah M, et al. Induction of neonatal tolerance by plasmid DNA vaccination of mice. J Clin Invest,1996,98:2700-2705.
132. Movsesyan N, Mkrtichyan M, Petrushina I, et al. DNA epitope vaccine containing complement component C3d enhances anti-amyloid-beta antibody production and polarizes the immune response towards a Th2 phenotype. J Neuroimmunol,2008, 205:57-63.
133. Myung K H. Effect of recombinant bovine somatotropin on milk production and milk composition in dairy cows. Asian Austral JAnim,1990,3:247-252.
134. Nakayama K, Kelly S M, Curtiss R 3rd. Construction of an asd+ expression-cloning vector:stable maintenance and high level expression of cloned genes in a Salmonella vaccine strain. Biotechnology,1988,6:693-697.
135. National Research Council. Nutrient Requirements of Dairy Cattle.7th rev. ed. Natl. Acad. Sci., Washington, DC,2001.
136. Nogueira L A G, Pinheiro L E L, Norte A L, et al. Uterine involution and return to cycling ovarian activity in zebu cows. Revista Brasileira De Reproducao Anima, 1993,17:49-56.
137. Norman H D, Wright J R, Hubbard S M, et al. Reproductive status of Holstein and Jersey cows in the United States. J Dairy Sci,2009,92:3517-3528.
138. Okano A, Tomizuka T. Ultrasonic observation of postpartum uterine involution in the cow. Theriogenology,1987,27:369-376.
139. Pillon D, Caraty A, Fabre-Nys C, et al. Regulation by estradiol of hypothalamic somatostatin gene expression:possible involvement of somatostatin in the control of luteinizing hormone secretion in the ewe. Biol Reprod,2004,71:38-44.
140. Pizzi F, Rizzi R, Maltecca C, et al. Fertility and longevity in the Reggiana cattle breed. Ital J Anim Sci,2003,2(suppl.1):151-153.
141. Pryce J E, Royal M D, Garnsworthy P C, et al. Fertility in the high-producing dairy cow. Livest Prod Sci,2004,86:125-135.
142. Pryce J E, Veerkamp R F. The incorporation of fertility indices in genetic improvement programmes. Occ Publ Br Soc Anim Sci,2001,26:237-250.
143. Ralph M W. Biology of the uterus.2nd ed. New York:Plenum Press,1977.
144. Rao A V N, Babu M S, Rao H R M. Evaluation of fertility traits in Jersey cows. Indian Vet J,1995,72:939-942.
145. Reisine T. Somatostatin. Cell Mol Neurobiol,1995; 15(6):597-614.
146. Rekwot P I, Ogwu D, Oyedipe E O. Influence of bull biostimulation, season and parity on resumption of ovarian activity of zebu (Bos indicus) cattle following parturition. Anim Reprod Sci,2000,63:1-11.
147. Ren SG, Kim S, Taylor J, et al. Suppression of rat and human growth hormone and prolactin secretion by a novel somatostatin/dopaminergic chimeric ligand. J Clin Endocrinol Metab,2003,88(11):5414-5421
148. Robertson J S. Safety considerations for nucleic acid vaccines. Vaccine,1994,12: 1526-1528.
149. Roche J F. The effect of nutritional management of the dairy cow on reproductive efficiency. Anim Reprod Sci,2006,96:282-296.
150. Romero J J, Canas R, Baldwin R L. A technique for estimating milk production in rats. JNutr,1975,105:413-420.
151. Royal M D, Darwash A O, Flint A P F, et al. Declining fertility in dairy cattle: changes in traditional and endocrine parameters of fertility. Anim Sci,2000,70: 487-501.
152. Riidiger N S, Gregersen N, Kielland-Brandt M C. One shortwell conserved region of Alu-sequences is involved in human gene rearrangements and has homology with prokaryotic chi. Nucleic Acids Res,1995,23:256-260.
153. Sa Filho M F, Ayres H, Ferreira R M, et al. Equine chorionic gonadotropin and gonadotropin-releasing hormone enhance fertility in a norgestomet-based, timed artificial insemination protocol in suckled Nelore (Bos indicus) cows. Theriogenology,2010,73:651-658.
154. Sampson D A and Jansen G R, Measurement of milk yield in the lactating rat from pup weight and weight gain. J Pediatr Gastroenterol Nutr,1984,3:613-617.
155. Samuelsson B, Uvnas-Moberg K, Gorewit R C, et al. Profiles of the hormones somatostatin, gastrin, CCK, prolactin, growth hormone and cortisol. I. In dairy cows that are milked and fed separately or milked and fed simultaneously. Livest Prod Sci, 1996,46:49-56.
156. Savio JD, Boland M P, Roche J F. Development of dominant follicles and length of ovarian cycles in postpartum dairy cows. J Reprod Fertil,1990,88:581-591.
157. Scrable H, Stambrook P J. A genetic program for deletion of foreign DNA from the mammalian genome. Mutat Res,1999,429(2):225-237.
158. Shata M T, Stevceva L, Agwale S, et al. Recent advances with recombinant bacterial vaccine vectors. Molecular Medicine Today,2000,6:66-71.
159. Sheldon I M, Dobson H. Effect of administration of eCG to postpartum cows on folliculogenesis in the ovary ipsilateral to the previously gravid uterine horn and uterine involution. J Reprod Fertil,2000,119:157-163.
160. Sheldon I M, Dobson H. Postpartum uterine health in cattle. Anim Reprod Sci,2004, 82-83:295-306.
161. Sheldon I M. The postpartum uterus. Vet Clin NAm-Food A,2004,20:569-591.
162. Shrestha H K, Nakao T, Suzuki T, et al. Relationships between body condition score, body weight, and some nutritional parameters in plasma and resumption of ovarian cyclicity postpartum during pre-service period in high-producing dairy cows in a subtropical region in Japan. Theriogenology,2005,64:855-66.
163. Simpson R B, Chase C C Jr, Spicer L J, et al. Effect of exogenous insulin on plasma and follicular insulin-like growth factor Ⅰ, insulin-like growth factor bindingprotein activity, follicular oestradiol and progesterone, and follicular growth in superovulated Angus and Brahman cows. J Reprod Fertil,1994,102:483-492.
164. Sinclair K D, Kuran M, Gebbie F E, et al. Nitrogen metabolism and fertility in cattle: Ⅱ. Development of oocytes recovered from heifers offered diets differing in their rate of nitrogen release in the rumen. J Anim Sci,2000,78:2670-2680.
165. Spicer L J, Alpizar E, Echternkamp S E. Effects of insulin, insulin-like growth factor I, and gonadotropins on bovine granulose cell proliferation, progesterone production, estradiol production and or insulin-like growth factor-I production. J Anim Sci,1993,71:1232-1241.
166. Sun S S, Jang K, Kuk K, et al. The effects of somatostatin passive immunization on milk yield, plasma hormone and metabolite concentrations in rats. Asian Austral J Anim,1997,10:324-328.
167. Sun S S, Myung K H, Spencer G S G. Influence of endogenous somatostatin on lactation in rats. Asian Austral J Anim,1996,9(3):337-340.
168. Tanaka T, Arai M, Ohtani S, et al. Influence of parity on follicular dynamics and resumption of ovarian cycle in postpartum dairy cows. Anim Reprod Sci,2008, 108:134-143.
169. Temin H M. Overview of biological effects of addition of DNA moleculars to cells. J Med Virol,1990,31:13-17.
170. Ulmer J B, Wahren B, Liu M A. DNA vaccines:recent technological and clinical advances. Discov Med,2006,6(33):109-112
171. Van Vugt H H, Swarts H J, Van de Heijning B J, et al. Centrally applied somatostatin inhibits the estrogen-induced luteinizing hormone surge via hypothalamic gonadotropin-releasing hormone cell activation in female rats. Biol Reprod,2004,71:813-819.
172. Vanholder T, Leroy J L, Dewulf J, et al. Hormonal and metabolic profiles of high-yielding dairy cows prior to ovarian cyst formation or first ovulation post partum. Reprod Domest Anim,2005,40:460-467.
173. Wagner W C, Hansel W. Reproductive physiology of the post partum cow. I. Clinical and histological findings. J Reprod Fertil,1969,18(3):493-500.
174. Waterston A M, Gumbrell L, Bratt T, et al. Phase I study of TNF-a Auto VaccIne in Patients with metastatic cancer. Cancer Immunol Immunother,2005,54:848-857
175. Westwood C T, Lean I J, Garavin J K, et al. Effects of genetic merit and varying dietary protein degradability on lactating dairy cows. J Dairy Sci,2000, 83:2926-2940.
176. Xue C L, Mao D G, Yang L G, et al. Effect of gene adjuvant CpGDNA on the somatostatin DNA vaccine in mice. Scientia Agricultura Sinica,2007; 40(4):834-41.
177. Yi I, Lim D, Kim J, et al. Effects of active immunization against somatostatin or somatostatin analogues on milk production in rats. Nutr Res,1999,19(7):1061-1072.
178. Zain A E, Nakao T, Raouf M A, et al. Factors in the resumption of ovarian activity and uterine involution in postpartum dairy cows. Anim Reprod Sci,1995,38: 203-214.
179. Zulu V C, Sawamukai Y, Nakada K, et al. Relationship among insulin-like growth factor-Ⅰ, blood metabolites and postpartum ovarian function in dairy cows. J Vet Med Sci, 2002,64:879-885.