摘要
白蜡窄吉丁(Agrilus planipennis Fairmaire)是白蜡属树木的毁灭性蛀干害虫,自然分布于东北亚地区,近年来传播到美国和加拿大。目前在国内外一些地区危害较重。
为利用天敌控制白蜡窄吉丁的危害,对该虫寄生性天敌的生物学作了研究。测定了白蜡窄吉丁及2寄生性优势天敌白蜡吉丁柄腹茧蜂(Spathius agrili Yang)和白蜡吉丁啮小蜂(Tetrastichus planipennisi Yang)在越冬阶段的过冷却点和冰点。对新种白蜡吉丁肿腿蜂(Scleroderma pupariae Yang et Yao)的生物学、行为学和繁育技术进行了研究。主要结论如下:
白蜡窄吉丁的过冷却点在-26.38~-23.04℃之间,冰点在-21.72~-11.64℃之间;白蜡吉丁柄腹茧蜂的过冷却点为-26.28℃,冰点为-22.50℃;白蜡吉丁啮小蜂的过冷却点为-24.48℃,冰点为-21.87℃。结合当地的气候条件探讨了白蜡窄吉丁及其天敌可能的地理分布范围,并认为越冬对种群数量的影响较小。
白蜡吉丁肿腿蜂是白蜡窄吉丁幼虫和蛹的一种有效寄生蜂,该蜂1年发生5代,世代重叠严重,自然寄生率为13.9%,雌性成蜂不补充营养的条件下平均寿命可达27.38±0.43天,雄性成蜂平均寿命为12.43±0.35天。性比(♀:♂)为18.9~49.5:1,第一代雌性有翅率最高为56.6%。有孤雌生殖现象,其后代全部为雄性。在室内自然变温条件下,卵、幼虫、茧蛹期和全世代的发育起点温度分别为16.89±0.79、17.03±1.42、16.90±1.68和15.31±0.47℃。有效积温分别为33.82±4.13、49.11±7.93、128.88±27.87和277.00±14.15日.度。根据当地的气候预测该蜂每年可发生4.75代,与实际相符。
白蜡吉丁肿腿蜂的寄主有白蜡窄吉丁各龄幼虫、合欢吉丁幼虫、苹果小吉丁幼虫和蛹、花椒窄吉丁幼虫、核桃窄吉丁幼虫、麻天牛幼虫、黄粉虫蛹、光肩星天牛低龄幼虫、芦螟幼虫和蛹、复纹狭天牛幼虫、一种苹果天牛幼虫。白蜡吉丁肿腿蜂种的行为学复杂,对寄主有较强的搜索力和攻击力,其搜索机制可能主要是来自于寄主虫粪的气味,与寄主本身发出的气味关系可能不大。该蜂的茧在8±2℃下贮藏74天后,羽化率不到10%。成蜂在8±2℃下贮藏78天后,雄性全部死亡,雌性有滤纸或蜂蜜水保湿的情况下死亡率为5.0~41.7%,不保湿的情况下死亡率为0,成蜂经过低温贮藏与否对后代的产卵、出蜂量没有显著的差异。
用白蜡吉丁蛹做寄主人工繁育该蜂表明,雌蜂一生能产卵3~5次,每次的平均出蜂量为32.9±1.2头。采用虫蜂比为1:2的繁殖方法出蜂量大于的1:1接蜂方法。用酒精处理寄主对产卵、羽化率和出蜂量没有显著的影响。
Agrilus planipennis Fairmaire (EAB) is an important destroyed wood-boring beetle attacking ash trees, and natural distributed northeast Asia. In recent years, it spread American and Canada. At present, this pest outbreak domestic and abroad, therefore, lead to enormous economic and ecology damage.
In order to use natural enemies control EAB, the biological of enemies were studied. The super-cooling point and freezing point, in overwintering, of Spathius agrili Yang and Tetrastichus planipennisi Yang were determined. Scleroderma pupariae Yang et Yao was a new species and its biological, behavior and rearing techniques were researched. The mainly work and results of this paper are as follow:
Results indicated that the super-cooling point and freezing point of EAB were ranged from -26.38 to -23.04℃and from -21.72 to -11.64℃respectively. The super-cooling point and freezing point of S. agrili were -26.28℃and -22.50℃, and T. planipennisi were 24.48℃and -21.87℃respectively. According to local climate that we predicted low temperature in winter is a little influence to their overwintering population.
S. pupariae, ectoparasitoid of emerald ash borer (EAB) pupa, is a new effective natural enemy to EAB. It has 5 generations a year, and the generations overlaps heavily. Natural parasitod rate was 13.9%. The female adults average longevous were about 27.38±0.43 days without any nutrition supplementation and male adult were about 12.43±0.35 days. The sex ratio(♀:♂) was about 18.9~49.5:1. The female alate rate was different in every generation, and the rate in first generation was higher than in any other generation, it could reach 56.6%. Wasps parthenogenesis was observed without amphimixis, and their generations were all males.
The development threshold temperature and effective accumulated temperature of S. pupariae were examined in room temperature. Results showed that the development threshold temperature of egg, larval, pupal stages and the whole generation were 16.89±0.79, 17.03±1.42, 16.90±1.68 and 15.31±0.47℃, respectively. The effective accumulated temperatures for these stages were 33.82±4.13, 49.11±7.93, 128.88±27.87 and 277.00±14.15 degree-day, respectively. According to local climate we predicted that this wasp may produce 4.75 generations one year, which was consistent with the practice.
Host range of S. pupariae were determined in laboratory. The results showed that the wasp had a wide host range, including larva of EAB, Agrilus viduus, Agrilus mali, Agrilus zanthoxylumi, Agrilus lewisiellus, Falderman gebleri, Tenebrio molitor, Anoplophphora glabripennis, Chilo luteellus, Oatrinia orientalis, stenhomalus complicates, and another long-horned beetle. Pupa of EAB, Agrilus mali, Tenebrio molito and Chilo luteellus. The behaviour of S. pupariae was very complicated. The storage techniques and searching mechanism of S. pupariae were studied. Results indecated that wasps had stronger ability to search and attack the hosts, and its search mechanism possibly by identifying the hosts frass smell and had no relations with the smell that host larva sent out. The emergence rate was no more than 10 percent of the cocoon after stored 74 days in 8±2℃. After stored in 8±2℃for 78 days, male adults were all dead, and the death rate of female adults was 5.0~41.7 percent by using the water to keep humid, but without such condition, the death rate of female adults was 0. Low temperature storage had no significant difference in contrast to storage under room condition on their generations between oviposition and emergence number.
The artificial rearing techniques were also researched. The results showed that female adults oviposited 3~5 times in their lifetime, and average emergence wasps were 32.9±1.2, in using pupa of EAB as host. The rearing method which inoculated one wasp or two to the pupa in the tube was studied, and the results indicated that the amount of propagation with the former method was more than the amount with the latter one. The amount of oviposition and emergence with the treatment to host by alcohol had no difference with the control group.
引文
[1] Haack R. A., Jendek E., Liu H. P., et al. The emerald ash borer: a new exotic pest in North America[J]. Newsletter of Michigan Entomological Society, 2002,47(3 - 4):1~5
[2] Jendek E. Studies in the East Palaearctic species of the genus Agrilus Dahl, 1823 (Coleoptera: Buprestidae) [J]. Part I. Entomological Problems 1994,25(1):9~25
[3] 赵汗青,王小艺,杨忠岐,等.检疫性害虫——白蜡窄吉丁[J].植物检疫,2006,20(2):89~91
[4] 刘义果.沈阳地区白蜡窄吉丁的研究[J].沈阳市园林科学研究所内部刊物,1966
[5] 金若忠,栾庆书,云丽丽,等.花曲柳窄吉丁生物学调查[J].辽宁林业科技,2005,(5):22~24
[6] 刘海清,马润生,李庆海.梣小吉丁虫的调查研究及防治技术[J].天津农林科技,1996,1(135):46~48
[7] 尹鸿刚.利用天敌控制林业有害生物的发展思路[J].天津农林科技,2004,(2):9~10
[8] Scarrt T. A., McCullough D. G., Howse G. M. Forest Health Alert-3(Agrilus planipennis)-Emerald Ash Borer[R]. Canadian Food Inspection Agency, 2002
[9] McCullough D. G., Roberts D. L. Emerald Ash Borer[J]. USDA Forest Service, Northeastern Area State and Private Forestry[J], Pest Alert NAPR-07-02.2002
[10] 新种描述文献待发表
[11] Wei X, Reardon D., Wu Y, et al. Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), in China: a review and distribution survey[J]. 昆虫学报, 2004,47(5):679~685
[12] 刘桂军,刘恩山.花曲柳窄吉丁生活史及天敌的观察和防治[J].天津农林科技,2004,4(2):20~22
[13] 萧刚柔主编.中国森林昆虫(第二版)[M].北京:中国林业出版社,1992,400~401
[14] Herms D. A.; Stone A. K.; Chatfield J. A. Emerald ash borer: the beginning of the end of ash in North America? [J]. Special Circular - Ohio Agricultural Research and Development Center. Ohio Agricultural Research and Development Center, USA: 2004,(193):62~71
[15] 中国科学院动物所.中国农业昆虫(上册)[M],北京:农业出版社,1986,445~446
[16] 张良玉,陈周羡,杨国栋,等.绒毛白蜡窄吉丁虫防治技术研究[J].园林科技通讯,1995,4(1):1~7
[17] 赵同海,高瑞桐,LIU H P,等.花曲柳窄吉丁的寄主植物范围、危害和防治对策[J].昆虫学报, 2005, 48(4) :594~599
[18] 刘海军,温俊宝,骆有庆,等.北美地区一新外来入侵种-花曲柳窄吉丁[J].昆虫知识, 2005, 42(3): 348~352
[19] 王小艺,杨忠岐,刘桂军,等.白蜡窄吉丁幼虫的龄数和龄期测定[J].林业科学,2005,41(3):97~102
[20] 王小艺.白蜡窄吉丁的生物学及其生物防治研究[D].北京:中国林业科学研究院博士后研究工作报告,2005
[21] Wang X Y, Yang Z Q, Strazanac, J.C, et al. The biology and ecology of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) in China[J]. Journal of Entomological Science, (Accept, inpress)
[22] United States, Department of Agriculture Forest Service Northeastern Area State and Private Forestry[J]. Pest alert, NA-PR-02-04,2004,11
[23] Invasive species: how to identify emerald ash borer[J].Journal of Forestry, 2004,(102):5,4~5
[24] 赵同海,迟成刚,高瑞桐,等.花曲柳窄吉丁在不同地区年生活史的补充研究[J].中国森林病虫,2005, 24(6):17~20
[25] Robert A. h., Eduard J., Liu H. P. et al. Entomol. Soc., 2002,(47):3~4
[26] 王小艺,忠岐.白蜡窄吉丁幼虫及其天敌在空间格局上的关系[J].应用生态学报, 2005,16(8): 1427~1431
[27] 魏霞,Wu Yun,Reardon Dick.等.花曲柳窄吉丁的空间分布[J].昆虫知识,2006,43(5):712~715
[28] 孙铁环,李庆海.天津市森林病虫害发生及防治对策[J].天津农林科技,2004,(2):1~4
[29] http://www.ly.lf.gov.cn/ArticleShow.asp?ArticleID=676 廊坊林业网站
[30] 国家林业局.检防函[2003]14 号――“国家林业局防止外来林业有害生物管理办公室关于颁发林业危险性有害生物名单的通知”.北京,2004
[31] http://www.bjnw.gov.cn/jqdt/nchjjsyzz/nchjzz/200607/t20060706_22541.html. 北京农网,关于发布北京市补充林业检疫性有害生物名单的通知
[32] 周明华,冉俊祥,杜国兴,等.绿梣树吉丁虫[J].植物检疫,2204,18(2):88~90
[33] Emerson D. Wood recyclers respond to beetle infestation[J]. BioCycle, 2005,46(7):22-24
[34] Muirhead J R, Leung B, Overdijk C, et al. Modelling local and long-distance dispersal of invasive emerald ash borer Agrilus planipennis (Coleoptera) in North America[J]. Diversity and Distributions, 2006,12(1):71~79
[35] http://www.invasive.org/eab/美国农业部林务局和动植物检疫局主办
[36] http://www.emeraldashborer.info/
[37] http://www.aphis.usda.gov/newsroom/content/2006/11/eab_fed_order.shtml. 美国农业部动植物检验检疫局
[38] T. Davis Sydnor, Matthew Bumgardner, Andrew Todd, et al. The Potential Economic Impacts of Emerald Ash Bore (Agrilus planipennis) on Ohio, U.S. [J]. Communities. Arboriculture & Urban Forestry, 2007,33(1):48~54
[39] Kenneth R. Marchant. Managing the Emerald Ash Borer in Canada. Emerald Ash Borer Research and Technology Development Meeting[C]. Pittsburgh Pennsylvania. 2005,9(26~27):3~4
[40] Robert A. Haack, Toby R. Petrice. Emerald Ash Borer Adult Dispersal[C]. Emerald Ash Borer Research and Technology Development Meeting,2004
[41] Robin A. J. Taylor., Therese M. Poland., Leah S. Bauer. et al. Is Emerald Ash Borer an Obligate Migrant. Emerald Ash Borer Research and Technology Development Meeting. Pittsburgh Pennsylvania. 2005,9(26~27):14
[42] Dobesberger E. J. Agrilus planipennis: Pest Risk Assessment-Plant Health Risk Assessment Unit, Nepean, Ontario, Canada. [R]. Canadian Food Inspection Agency, 2002
[43] 赵汗青,王小艺,杨忠岐,等.利用图像对白蜡窄吉丁的自动识别[J].植物检疫,2006,20(5):286~287
[44] http://www.aphis.usda.gov/newsroom/content/2006/11/eab
[45] Animal and Plant Health Inspection Service , United States Department of Agriculture. Questions and Answers: Emerald Ash Borer Quarantine[J]. Plant Protection and Quarantine, 2006,( 11):25~28
[46] McCullougu D. G., David C., Poland M. Control of Emerald Ash Borer[C]. Research and Technology Development Meeting. September30-October, 2003. Michigan, USA. 27~28
[47] Leais P. A. Control of Emerald Ash Borer: Direct Trunk Injection Technologies[C]. Emerald Ash Borer Research and Technology Development Meeting. September30-October, 2003. Michigan, USA. 29
[48] Leah S . Bauer., Liu H. P., Haack R. A., et al. Natural Enemies of Emerald Ash Borer in Southestern Michigan.Emerald Ash Borer[C]. Research and Technology Development Meeting. September 30-October1, 2003. Michigan, USA. 33~34
[49] 孙龙强.白蜡窄吉丁病原真菌调查与分离研究[D].北京:中国林业科学研究院硕士学位论文.2006,29-34
[50] Liu H P, Bauer L. S., Gao R T. Exploration survey for the Emerald Ash Borer,Agrilus planipennis (Coleoptera: Buprestidae),and its natural enemies in China[J]. The great lakes entomologist, 2003, 36(3&4):191~204
[51] Bauer, L. S., Liu H P., Haack R. A.,et al.Natural enemies of emerald ash borer in southeastern Michigan[J], pp 33-34. In: Emerald Ash Borer Research and Technology Development Meeting, Port Huron, MI, 30 September-1 October 2003. UDSA Forest Service, Fort Collins, CO. FHTET-2004-02
[52] Zhang Y Z, Huang D W, Zhao T H, et al. Two new species of egg parasitoids (Hymenoptera: Encyrtidae) of wood-boring beetle pests from China[J]. Phytoparasitica. 2005.33(3):253~260
[53] Mastro V., Reardon R. Emerald Ash Borer: Research and Technology Development Meeting,Romulus, Michigan, USDA Forest Service publication, 2004,5-6
[54] Yang Zhong-qi, Strazanac J S, Marsh P M, et al. First Recorded Parasitoid from China of Agrilus planipennis: A New Species of Spathius (Hymenoptera: Braconidae: Doryctinae)[J]. Annals of the Entomological Society of America, 2005,98(5):636~642
[55] Yang Zhong-qi, Strazanac, J.C, Yao Yan-xia, and Wang Xiao-yi.. A new species of emerald ash borer parasitoid from China belonging to the genus Tetrastichus Haliday (Hymenoptera: Eulophidae)[J]. Proceedings of the Entomological Society of Washington, 2006,108(3):550-558
[56] 王小艺,杨忠岐,刘桂军,等.白蜡吉丁柄腹茧锋的羽化和产卵与寄主之间的关系[J].生态学报, 2006, 26(4):1104~1109
[57] 王小艺,杨忠岐.白蜡窄吉丁生物学及其生物防治研究[C].全国生物防治会议论文集,上海,2005.4
[58] Francese, J. A. Mastro, V. C. Oliver, et al. Evaluation of colors for trapping Agrilus planipennis (Coleoptera: Buprestidae)[J]. Journal of Entomological Science. 2005,40(1):93~95
[59] Poland M., Peter G., Gary G.t et al. Developing Attractants and Trapping Techniques for the Emerald Ash Bore[C]r. Emerald Ash Borer Resesrch and Technology Development Meeting. September30-October, 2003. Michigan, USA. 15~16
[60] 李中新,刘玉升.寄生蜂的繁殖与利用进展[J].山东农业大学学报(自然科学版),2003,34(2):289~293
[61] 陕西省林科院,河南省林科院主编.林虫寄生蜂图志[M].陕西:天则出版社,1990
[62] 王大洲,师国洪.生物防治在林木害虫综合治理中地位的研究[J].河北林业科技,2005,(3):12~14
[63] 刘辉芳,王志刚,黄大庄.中国林木天牛天敌应用现状[J].河北农业大学学报卷增刊,2002,(25): 224~226
[64] 盛茂领.中国古北区林不钻蛀害虫天敌姬蜂(膜翅目:姬蜂科)分类研究[D].北京:北京林业大学博士论文,2005
[65] 杨伟,周祖基.我国天牛类害虫生物防治概况[J].四川林业科技,2001,22(3):49~53
[66] 赵修复主编.害虫生物防治[M].北京:中国农业出版社,2004,34~36,89~90
[67] 舒惠国,李保同著.生物防治概述[M].北京:中国人事出版社,2005,7~17,57~67
[68] Simmonds F. J. 1944. The propagation of insect parasites on unnatural hosts[J]. Bull. Entomol. Res. 35: 219~226
[69] 胡京生.昆虫寄生天敌人工饲养技术研究的现状及其展望.刘同先,康乐主编.昆虫学研究进展与展望[M].北京:科学技术出版社,2005,387~401
[70] 忻介六,苏德明等,编著.昆虫、螨类、蜘蛛德人工饲料[M].北京:科学出版社,1979,157~171
[71] 王延年,郑忠庆,周永生等,编著.等.昆虫人工饲料手册[M].上海:上海科学技术出版社,1984,237~250
[72] Nettle, W. C. Jr., Wilson. In vitro rearing of parasitoids: Role of host factors in nutrition. Arch[J]. Insect Biochem. Physiol,1990,(13):167~175
[73] Grenier, S., Greany, P. D., Cohen, A. C. Potential for mass release of insect parasitoids and predators through development of artificial cultural technique[M]. In: Rosen. D., Bennett, F. D., Capinera, J. L., Pest management in the subtropics. Biological control-a Florida perspective. Andover, England: Intercept Ltd,1994,181~205
[74] Bratti, A., Campadelli, G. In vitro rearing of Exerista larvarum on tissue culture basrd diets[J]. Entomol. Exp,1995,(74):47~53
[75] Hu, J. S., Vinson, S. B. The in vitro development from egg to prepupa of Campoletis somorensis (Hymenoptera: Ichnemonidae) in an artificial medium: important of physical factors[J].Insect Physiol,1998,(44):455~461
[76] Richerson, J. V. & Borden, J. H. Host finding behaviour of Coeloides brunneri (Hymenoptera: Braconidae)[J]. Canadian Entomologist,1972.(104):1235~1250
[77] 张连芹,宋世涵,范景祥.斑头陡盾茧蜂防治粗鞘双条杉天牛的研究[J].林业科学, 1987, 23(3): 306~313
[78] Millar J. G., Paine T. D., Campbell C. D. et al .Methods for rearing Syngaster lepidus and Jarra phoracantha(Hymenoptera: Braconidae), larval parasitoids of the phloem-colonizing longhorned beetles Phoracantha semipunctata and P. recurva (Coleoptera: Cerambycidae)[J]. Bulletin of entomological research, 2002,(92):141~146
[79] River A. The relationship between host selection behavior and offspring fitness in a koinobiont parasitoid. Ecological Entomology, 2000,( 25):467~472
[80] Joyce, A. L., Millar, J. G. et al. The effect of host size on the sex ration of Syngaster lepidus, a parasitoid of eucalyptus longhorned borer(Phoracantha spp.)[J],Biological control, 2002
[81] 萧刚柔,吴坚.防治天牛的有效天敌——管氏肿腿腿蜂[J].林业科学,1983(增刊):81~84
[82] 王功桂,周灵会,王长旭,等.管氏肿腿蜂防治松墨天牛技术[J].中国森林病虫,2005,23(3):32~34
[83] 皮忠庆,王福维,高立军,等.管氏肿腿蜂防治青杨天牛试验[J].中国森林病虫,2001,(6):20~22
[84] 冯建森,牛犇,王敦兴,等.甘肃引进管氏肿腿蜂防治青杨天牛有效性分析研究[J].甘肃林业科技, 2005,30(1):24~26,30
[85] 程惠珍,陈君,张国珍,等.应用天牛肿腿蜂防治菊天牛的初步研究[J].中国中药杂志, 1993, 18(12): 716~717
[86] 陈君,程惠珍.肿腿蜂的应用研究进展[J].中国生物防治,2000,16(4):166~170
[87] 张连芹,宋世函,范军祥.利用蜜蜂雄蛹繁殖管氏肿腿蜂[J].昆虫天敌,1984 ,6(4):244~247
[88] 张连芹,宋世涵,范军祥.大袋蛾繁殖管氏肿腿蜂的初步研究[J].生物防治通报,1987,3(3):114~116
[89] 程惠珍,卢美娟,林谦壮.用玉米螟作寄主繁殖管氏肿腿蜂[J].生物防治通报,1989,5(4):145~148
[90] 钱明惠.不同寄主繁育管氏肿腿蜂的初步研究[J].广东林业科技,1999,15(3):44~46
[91] 李理,温硕洋,谢以权,等.管氏肿腿蜂离体培养研究[J].昆虫天敌,1994,16(1):6~7
[92] 周娜,姚圣忠,胡德夫,等.管氏肿腿蜂的人工繁育与应用研究进展[J].干旱区研究,2005,22(4): 569~575
[93] 谢振东,张绪成,张佩勇,等.用栗山天牛幼虫做寄主人工繁殖管氏肿腿蜂的试验研究[J].吉林林业科技,1999(6) :11~12
[94] 田慎鹏,徐志强.不同温度条件对利用黄粉甲繁育管氏肿腿蜂的影响[J].昆虫知识, 2003,40(4):356~359
[95] 程彬,李晓颖,李贞淑.人工繁育管氏肿腿蜂几项技术指标的试验[J].吉林林业科技,1998,(5):19,52
[96] 邹庆旭,王日龙.肿腿蜂繁育及其应用技术[J].山西林业,2001,(5):24~25
[97] 宋世涵,范军祥.异地管氏肿腿蜂交配繁蜂防治粗鞘双条杉天牛的效果[J].生物防治通报, 1985, 1(4):39
[98] 萧刚柔.天牛的两种新寄生天敌——川硬皮肿腿蜂及海南硬皮肿腿蜂(膜翅目,肿腿蜂科 ) [J].林业科学研究,1995,(8) (专刊):1~5
[99] 周祖基,杨伟.川硬皮肿腿蜂生物学特性的研究(膜翅目:肿腿蜂科)[J].林业科学,1997,33(5): 475~480
[100] 肖银波,周建华,肖育贵,等.川硬皮肿腿蜂防治云斑天牛试验初报[J].四川林业科技, 2003, 24(4): 37~41
[101] 周祖基,曾垂惠,杨伟,等.川硬皮肿腿蜂环境适应性的初步研究[J].森林病虫通讯,1996,(3):4~6
[102] 周祖基.川硬皮肿腿蜂研究概述[J].四川林业科技,1999,20(3):59~61
[103] 杨德敏,曾垂惠,杨萍,等.三峡库区利用川硬皮肿腿蜂防治天牛类害虫[J].中国生物防治, 1999, 15(3):140~141
[104] 黄琼,胡杰,杨春平,等.贮藏条件对川硬皮肿腿蜂存活率的影响[J].昆虫知识,2006,43(5):673~677
[105] 曾垂惠,杨德敏,叶伟军.川硬皮肿腿蜂的人工繁育技术研究[J].四川林业科技,1997,18(3):14~17
[106] 杨德敏,曾垂惠,周祖基,等.川硬皮肿腿蜂中间寄主的人工繁育技术研究[J].中国森林病虫, 1998, (2):12~13,19
[107] 黄琼,周祖基,杨伟,等.繁育川硬皮肿腿蜂替代寄主的筛选[J].昆虫学报,2005,48(3):375~379
[108] 中国森林编辑委员会编著.中国森林第 3 卷阔叶林[M].北京:中国林业出版社,2000,1399~1408
[109] 杨忠岐.利用天敌昆虫控制我国重大林木害虫研究进展[J].中国生物防治,2004,20(4):221~227
[110] MacFarlane, D. W.; Meyer S. P. Characteristics and distribution of potential ash tree hosts for emerald ash borer[J]. Forest Ecology and Management, 2005.(1-3):15~24
[111] T. Davis Sydnor, Matthew Bumgardner, Andrew Todd, et al. The Potential Economic Impacts of Emerald Ash Bore (Agrilus planipennis) on Ohio, U.S. [J]. Communities. Arboriculture & Urban Forestry, 2007,33(1):48~54
[112] http://www.inspection.gc.ca/english/plaveg/pestrava/agrpla/mc/2006ontarioe.shtml 加拿大食品检疫局
[113] 张喆.国外花曲柳窄吉丁危害与防治现状简介[J].天津农林科技,2004,(2):27~28
[114] 天津市大港区地方史志编修委员会.大港区志(1) [M].天津,1998
[115] 中国区政大典编委会编著.中国区政大典(1) [M].杭州:浙江人民出版社,1998:1025
[116] 刘宁,文丽萍,何康来,等.不同地理种群亚洲玉米螟抗寒力研究[J].植物保护学保,2005,32(2): 163~168
[117] 陈子星,徐夕水.生物统计 SAS 程序题解[M].北京:中国农业科学院计算中心,1997
[118] 南京农学院主编.昆虫生态及预测预报[M].北京:农业出版社,1985:320~324
[119] 景晓红,康乐.昆虫耐寒性的测定与评价方法[J].昆虫知识,2004,41(1):7~10
[120] 杨忠岐.白蛾周氏啮小蜂的有效积温及发育起点温度研究[J].林业科学,2000,36(6):119~122
[121] 周祥,李世平,黄光斗,等.椰心叶甲啮小蜂发育起点温度和有效积温研究[J].昆虫天敌, 2006,28(1): 8~12