微生物絮凝剂产生菌的筛选及初步应用研究
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摘要
絮凝技术被广泛应用于给水、废水净化,污泥脱水处理中,其絮凝效果主要取决于絮凝剂种类。微生物絮凝剂是一类由微生物产生的代谢产物,主要成分有多糖、糖蛋白、蛋白质、纤维素和DNA等。作为一种新型的水处理剂,它不仅避免了无机、有机高分子絮凝剂的大量使用对环境和人类健康造成的危害,更具有高效、无毒、无二次污染的特点,对多种工业废水都有良好的净化效果,有着广泛的应用前景,因此受到各国研究者的重视。本论文在系统地论述近年来国内外微生物絮凝剂的研制开发现状与发展的基础上,对微生物絮凝剂产生菌种筛选、培养条件优化、微生物絮凝剂的絮凝特性以及应用,进行了研究与探讨,主要内容与所得结论如下:
(1)本试验以校园绿地土壤和陕西杨凌植化工程中心排水口污泥作为菌源,在特定的培养基中培养增殖,平板涂布得到单菌。以高岭土为测试对象,对获得的单菌株絮凝活性进行测定、筛选絮凝剂产生菌。在筛选得到的21株菌中,发现其中一株来源于土壤的菌株具有较强的絮凝活性,初步鉴定为细菌/芽孢杆菌科/芽孢杆菌属,并命名为Bacillus BC—11。
(2)为了最大限度地提高絮凝剂的絮凝活性,在筛选培养基的基础上,对培养基的组成及培养条件进行了优化。在优化后的培养条件下,絮凝剂产生菌能够在细胞内合成絮凝剂并分泌到胞外,生成具有絮凝活性的粘性微生物絮凝剂。
(3)对得到的产絮凝剂微生物的生长曲线进行研究发现,菌Bacillus BC—11在生长的同时产生并积累絮凝剂,将其分泌到细胞外,起絮凝作用的物质主要存在于发酵液中。
(4)为了降低生产成本,采用皂素废水作为替代培养基。在添加0.1%的NaNO3作为外加氮源和0.8%的KH2PO4,调节培养基pH值为7的条件下,对菌Bacillus BC—11进行培养,可以获得廉价的絮凝剂,培养得到絮凝剂的絮凝活性能达到82.57%,说明制药废水也能够做为替代培养基培养产絮凝剂微生物。
(5)应用研究结果表明,由菌Bacillus BC—11产生的絮凝剂,对土壤悬浊液,造纸废水,生活废水和水性油墨废水都有良好的处理效果。尤其是对以射光蓝废水为代表的水性油墨废水的处理效果最佳,其处理效果优于传统使用的无机絮凝剂。对水性油墨废水的处理方法国内外目前还鲜见报道。
Flocculants are widely used in wastewater treatment. The flocculating activity has big deal with the type of flocculants. Micorbial flocculants are newly developed natural macromolecular organic substance, which have the ability to flocculate various wastewater. For their biodegradability, high performance and harmlessness to the environment, microbial flocculants have drowned more and more attention, and become the front line of investigation. This paper summarized a series of basic research and development om preparation of microbial flocculants. This isolation of flocculant-producing bacterium from active sludge and soil, optimization of culture condition for flocculant production, pilot-scale, the composition, characteristic and flocculation mechanism of the microbial flocculants were studied and discussed systematically. The main centents of research and the results are as follows:
(1) For the screening of microbial flocculant-producing microorganism, suspensions from sludge and soil solution were cultivated in given media with Kaolin clay suspension as the test sample for flocculating activity. A flocculant-producinbg bacterium isolated from soil was identified as Bacillus BC—11.
(2) Culture medium and conditions for flocculant production were optimized on the basis of original screening culture medium. Under the optimal culture conditions, Bacillus BC—11 synthesized intracellularly and excreted extracellularly viscous flocculant immediately.
(3) Under the research of growth curve of Bacillus BC—11, the flocculating substances synthesized intracellularly which mainly existed in ferment liquid.
(4) In order to reduce the cost of media, the strain Bacillus BC—11 was cultured in wastewater of yam samponin. The results showed that the strain Bacillus BC—11 could grow and reproduce well, which provided technical and economic feasibility for further production of microbial flocculant on pilot-scale.
(5) The results of application studies indicated that the microbial flocculant produced by Bacillus BC—11 had the ability to flocculate various simulating wastewater and practical wastewater well, especially on the reflex blue wastewater.
(1)本试验以校园绿地土壤和陕西杨凌植化工程中心排水口污泥作为菌源,在特定的培养基中培养增殖,平板涂布得到单菌。以高岭土为测试对象,对获得的单菌株絮凝活性进行测定、筛选絮凝剂产生菌。在筛选得到的21株菌中,发现其中一株来源于土壤的菌株具有较强的絮凝活性,初步鉴定为细菌/芽孢杆菌科/芽孢杆菌属,并命名为Bacillus BC—11。
(2)为了最大限度地提高絮凝剂的絮凝活性,在筛选培养基的基础上,对培养基的组成及培养条件进行了优化。在优化后的培养条件下,絮凝剂产生菌能够在细胞内合成絮凝剂并分泌到胞外,生成具有絮凝活性的粘性微生物絮凝剂。
(3)对得到的产絮凝剂微生物的生长曲线进行研究发现,菌Bacillus BC—11在生长的同时产生并积累絮凝剂,将其分泌到细胞外,起絮凝作用的物质主要存在于发酵液中。
(4)为了降低生产成本,采用皂素废水作为替代培养基。在添加0.1%的NaNO3作为外加氮源和0.8%的KH2PO4,调节培养基pH值为7的条件下,对菌Bacillus BC—11进行培养,可以获得廉价的絮凝剂,培养得到絮凝剂的絮凝活性能达到82.57%,说明制药废水也能够做为替代培养基培养产絮凝剂微生物。
(5)应用研究结果表明,由菌Bacillus BC—11产生的絮凝剂,对土壤悬浊液,造纸废水,生活废水和水性油墨废水都有良好的处理效果。尤其是对以射光蓝废水为代表的水性油墨废水的处理效果最佳,其处理效果优于传统使用的无机絮凝剂。对水性油墨废水的处理方法国内外目前还鲜见报道。
Flocculants are widely used in wastewater treatment. The flocculating activity has big deal with the type of flocculants. Micorbial flocculants are newly developed natural macromolecular organic substance, which have the ability to flocculate various wastewater. For their biodegradability, high performance and harmlessness to the environment, microbial flocculants have drowned more and more attention, and become the front line of investigation. This paper summarized a series of basic research and development om preparation of microbial flocculants. This isolation of flocculant-producing bacterium from active sludge and soil, optimization of culture condition for flocculant production, pilot-scale, the composition, characteristic and flocculation mechanism of the microbial flocculants were studied and discussed systematically. The main centents of research and the results are as follows:
(1) For the screening of microbial flocculant-producing microorganism, suspensions from sludge and soil solution were cultivated in given media with Kaolin clay suspension as the test sample for flocculating activity. A flocculant-producinbg bacterium isolated from soil was identified as Bacillus BC—11.
(2) Culture medium and conditions for flocculant production were optimized on the basis of original screening culture medium. Under the optimal culture conditions, Bacillus BC—11 synthesized intracellularly and excreted extracellularly viscous flocculant immediately.
(3) Under the research of growth curve of Bacillus BC—11, the flocculating substances synthesized intracellularly which mainly existed in ferment liquid.
(4) In order to reduce the cost of media, the strain Bacillus BC—11 was cultured in wastewater of yam samponin. The results showed that the strain Bacillus BC—11 could grow and reproduce well, which provided technical and economic feasibility for further production of microbial flocculant on pilot-scale.
(5) The results of application studies indicated that the microbial flocculant produced by Bacillus BC—11 had the ability to flocculate various simulating wastewater and practical wastewater well, especially on the reflex blue wastewater.
引文
[1] 国家环境保护局.混凝剂与絮凝剂[M].北京:中国环境科学出版社,1991,1
[2] 周群英,高延耀.环境工程微生物学[M].第二版.北京:高等教育出版社,2000,7
[3] 陆柱,蔡兰坤,陈中兴等.水处理药剂[M].北京:化学工业出版社,2002
[4] 崔子文,郝红英.水处理中絮凝剂的研究应用现状[J].山西化工,1999, 19 (1 ):58-61
[5] 马青山,贾瑟,孙丽珉等.絮凝化学和絮凝剂[M].第 1 版.北京:中国环境科学出版社,1990, 1-115
[6] American Water Works Association Coagulation Committee. Committee Report:coagulation as an integrated water treatment process. J Am Water Works Assoc[J], 1989,81(10):72
[7] 姚重华.混凝剂与絮凝剂[M].北京:中国环境科学出版社,1992.
[8] 李琼,肖锦,肖晶.接枝共聚型天然高分子絮凝剂的性能及应用研究[J].工业水处理,1997, 17 (4): 27-29
[9] 陶涛,卢秀清,冷静.微生物絮凝剂研究与应用进展[J].环境科学进展,1999(7): 21—25.
[10] 张育新,康勇.絮凝剂的研究现状及发展趋势[J].化工进展,2002, 21(11):799—804
[11] 常青.水处理絮凝学[M].北京:化学工业出版社,2003
[12] Dentel SK, Gossett JM. Mechanisms of coagulation with aluminums aits. J Am Water Works Assoc[J], 1988,80(4):187
[13] 吴健,戴桂馥.微生物细胞的絮凝与微生物絮凝剂[J].环境污染与防治,1994,16(6):27-32.
[14] 胡勇有,高健.微生物絮凝剂的研究与应用进展[J].环境科学进展,1999, 7(4):24-28
[15] 汤鸿宵.无机高分子复合絮凝剂的研制倾向[J].中国给水排水,1999, 15 (2):1-4
[16] 崔蕴霞,肖谨.铝盐絮凝剂及其环境效应[J].工业水处理,1998,18(3):6—9
[17] 吕新之.铝的污染和危害[J].环境保护,1998,5:38—39
[18] 金晓红,任华炜.环境生物技术应用与环境保护的新进展[J].环境保护,2002,2:18—19
[19] 郑怀礼等. 生物絮凝剂与絮凝技术[M]. 北京:化学工业出版社,2003. 11
[20] 马放,冯玉杰,任南琪等.环境生物技术[M]. 北京:化学工业出版社,2003.4
[21] 张彤,朱怀南,林哲.微生物絮凝剂的研究与应用进展[J]. 应用与环境生物学报,1996,(1):95—105
[22] 李桂娇,尹华,彭辉.生物絮凝剂的研究与开发[J]. 工业水处理,2002,22(3):9—12
[23] 陈坚,任洪强,堵国成等. 环境生物技术应用与发展[M].北京:中国轻工业出版社,2001
[24] Lee SH,Lee SO,Jang KL. Microbial flocculant from Arcuadendrom SP. TS-49[J]. Biotechnology Letters,1995,17(1):95—100
[25](日)有马启等.生物净化环境技术[M].化学工业出版社.北京,1990
[26] 马放,杨基先,金文标等编著.环境生物制剂的开发与应用[M].北京:化学工业出版社,2004.3
[27] 周少奇编著.环境生物技术[M].北京:科学出版社,2003.7
[28] Butterfield CT. Studies of sewage purification Ⅱ a Zooloea-forming bacterium isolated from activated sludge[J]. U.S.public Health rep, 1935,50:671
[29] Junji Nakamura. Purification and Chemical Analysis of Microbial Cell Flocculant produced by Aspergillus sojae AJ7002[J]. Agri. Biol. Chem,1976,40(3):619—624
[30] Takagi H, hadowaki K. Flocculant production by Paecilomyces sp. Taxonomic studies and culturecondition for production[J]. Agri. Biol. Chem, 1985,49(11):3151—3157
[31] Minoru Takeda, Ryuichiro Kurane. A Protien Bioflocculant Produced by Rhodococcus erythropolis[J].Agri.Biol.Chem,1951,55(10):2663—2664
[32] Suh H-H,et al. Characterication of bioflocculant produced by Bacillus sp. DP-152[J].Journal of fermentation and Bioengineering,1997,84(2): 108—112
[33] 王镇,王孔星. 几株絮凝剂产生菌的特性研究[J]. 微生物学报, 1995, 35 (2):121—129
[34] 马放,刘俊良,李淑更等.复合型微生物絮凝剂的开发[J].中国给水排水,2003,19(4):1—4
[35] 李剑,王曙光,高宝玉等.利用乳品废水生产微生物絮凝剂及其应用研究[J].环境工程,2004,22(6):93—94
[36] 王曙光,李剑,高宝玉等.一株产气肠杆菌絮凝特性的研究[J].环境化学,2005,24(2):171—174
[37] Kurane R, Takda K, Suzuki T. Screening for and characteristics of microbial flocculants[J]. Agric Biol Chem,1986,50(9):2301-2307
[38] Nakamura J, Miyashiro S, Hirose Y.Screening,isolation,and some properties of microbial cell flocculants[J]. Agric. Biol. Chem, 1976,40(2):377-383
[39] Sakka K, Takahashi H. Deoxyribonuclease-susceptible floc. forming Pseudomonas sp.[J] Agric Biol Chem,1981,45(12):2869-2876
[40] Takagi H, Kadowaki K. Purification and chemical properties of a flocculant produced by Paecilomyces[J]. Agric. Biol.Chem., 1985. 49( 11):3159-3164
[41] 周旭,黄丽萍,王竞等.利用鱼粉废水生产生物絮凝剂及其性能研究[J].应用与环境生物学报,2003, 9 (4): 436-438
[42] 石璐,刘忠义,唐受印等.微生物絮凝剂产生菌的筛选及培养条件优化[J].环境科学研究,2003, 16 (3): 35-36
[43] 邓述波,胡筱敏,罗茜.寄生曲霉产生絮凝剂的培养条件及其絮凝特性[J].应用与环境生物学报,1998, 4 (4) : 405-408
[44] 何宁,李寅,陆茂林等.营养和环境条件对生物絮凝剂合成的影响[J].应用与环境生物学报,2001, 7 (5): 483-488
[45] 秦培勇等.微生物絮凝剂及其絮凝机理的研究进展[J].内蒙古工业大学学报,2001, 20(3):166-170
[46] 刘紫鹃等.絮凝剂 BP25 的化学组成及结构研究[J].微生物学通报,2001,41 (3):348-352
[47] J.Nakaura,S.Miygeyshi,Y Hirose.Conditions for production of microbial cell flocculant by Aspergillus sojae AJ7002[J]. Agr.Biol.Chem.,1976 40 (7): 1341-1347
[48] 傅旭庆,汪大挥.微生物絮凝剂及其絮凝机理[J].污染防治技术,1998,11(1):6-9
[49] Zhang Tong, Lin Zhe. Microbial Flocculant and Its Application in Environmental Protection[J]. Environmental Sciences. 1999,(1): l—12
[50] 王猛,施宪法,柴晓利.微生物絮凝剂的研究与应用[J].化工环保,2001,21(6):328-332
[51] 翟广飞.产絮凝剂微生物的特征及其培养条件研究[D].武汉理工大学,2003,5
[52] 石璐. 高效微生物絮凝剂产生菌的筛选及培养条件研究[D].湘潭大学,2003,5
[53] 宫小燕,王曙光,栾兆坤等 微生物絮凝剂产生菌的筛选和优化以及在水处理中的应用[J]. 应用与环境生物学报,2003,9(2):196—199
[54] 程丽娟,薛泉宏主编.微生物学实验技术[M].世界图书出版公司,2004,4
[55] 石璐,刘忠义,唐受印等.微生物絮凝剂产生菌的筛选及培养条件优化[J].环境科学研究,2003,16(3):35—36,58
[56] Kurane R, Todea K, Takeda K.Culture conditions for production of microbial flocculant by Rhodococcus erythropolis[J]. Agri. Biol. Chem.,1986,50(9):2309—2313
[57] R.E.步坎南,N.E.吉本斯等. 伯杰细菌鉴定手册(第八版)[M]. 北京:科学出版社,1984,12
[58] Salehizadeh H, Shojaosadati S A. Extracellular biopolymeric flocculants Recent trends and biotechnological importance. Biotechnology Advances,2001,19:371~385
[59] Kurane R, Nohata Y. Microbial Flocculation of Waste Liquids and Oil Emulsion by a Bioflocculant from Alcaligenes latus. Agric Biol Chem,1991,44: 1127~1129
[60] Todea K, Kurane R. Microbial flocculant from Alcaligenes cuipdus KT201[J]. Agri. Biol. Chem.,1991,55(11):2793—2799
[61] Kazuki T, Kurane R. Microbial Flocculant from Alcaligenes cupidus KT201[J]. Agric Biol Chem,1991,55(11):2793—2799
[62] kakii K,Sugahara E,Shirakashi T. isolation and characterization of a Ca+-dependent floc-forming bacterium[J].Ferment.Technol.,1986,64(1):57—62
[63] Takakazu Endo, Kazuo Nakamura, Hajime Takahashi. Pronase—susceptible Floc Forming Bacteria : Relationship between Flocculation and Calcium Ion[J]. Agric Biol Chem, 1976,40 (11): 2289—2295
[64] Kurane R, Hatamochi K, Kakuno T. Purification and Characterization of Lipid Bioflocculant Produced by Rhodococcus erythropoils[J]. Biosci Biotech Biochem, 1994,58(11): 1977—1982
[65] 沈同,王镜岩.生物化学[M].第二版.北京
[66] Kazui S, Hajime T. DNA as a flocculation factor in Pseudomonas sp[J]. Agric. Biol. Chem., 1981 , 45 (12): 2869—2876
[67] Sousa M J, Texeira J A, Mota M. Difference in the flocculation mechanism of Kluyveromyces marxianus and Saccharomyces cerevisiae[J]. Biotechnology Letters, 1992, 14 (3): 213—218
[68] Salehizadeh H, Shojaosadati S A. Extracellular biopolymeric flocculants Recent trends and biotechnological importance[J]. Biotechnology Advances,2001,19:371—385
[69] 宫小燕,栾兆坤,王曙光等. 微生物絮凝剂絮凝特性的研究[J]. 环境化学,2001,20(6):550—556
[70] 李剑,冯贵颖,王曙光等. 絮凝剂产生菌 KLE—1 的特性研究[J]. 西北农林科技大学学报,2004,32(增刊):121—124
[71] Tezuka Y. Cation—dependent flocculation in a flavobacterium species predominant in activated sludge[J]. Applied Microbiology, 1969, 17 (2): 222—226
[72] Endo T,Nakumaru K,Takahashi H. Pronase-susceptible floc forming bacteria:relationship between flocculation and calcium ion. Agri.Biol.Chem.,1976,40(11):2289—2295
[73] Sousa M J, Texeira J A, Mota M. Difference in the flocculation mechanism of Kluyveromyces marxianus and Saccharomyces cerevisiae[J]. Biotechnology Letters, 1992, 14 (3): 213—218
[74] Bar-or Y and Shilo M. Chrarcterization of macromolecular flocculants produced by Phormidium sp. Strain J-1 and by Anabaenopsis circularis PCC6720[J].Applied and Environmental Microbiology,1987,53(9):2226—2230
[75] Choi CW,Yoo SA,Oh IH,Park SH. Characterization of an extracellular flocculating substance produced by a planktonic cyanobacterium, Anabaena sp.[J], Biotechnology Letters,1998,20(7):643—646
[76] 刘春. 皂素生产废水污染特点及治理对策探讨[J]. 环境保护科学,2001,27(6):22—24
[77] 单丽伟,冯贵颖,朱丹等. 上流式厌氧污泥床(UASB)处理皂素废水的研究[J]. 西北农林科技大学学报,2003,31:109—112
[78] 朱丹,冯贵颖,呼世斌等. 皂素废水处理中厌氧污泥活性影响因素的研究[J]. 西北农林科技大学学报,2005,33(12):103—106
[79] 周德庆. 微生物学教程. 第一版. 北京:高等教育出版社,1995
[80] 孔繁翎. 环境生物学. 北京:高等教育出版社,2002,5
[81] Ning He, Yin Li, Jian Chen, Shi-Yi. Identification of a novel bioflocculant from a newly isolated Coynebacterium glutamicum[J]. Biochemical Engineering Journal,2002,11:137—148
[82] Kurane R, Hatamochi K. Chemical structure of lipid bioflocculant produced by Rhodococcus erythropolis[J]. Agri.Biol.Chem.,1995,59(9):1652—1656
[83] 袁志发,周静芋. 试验设计与分析[M].北京,高等教育出版社,2000,8
[84] Kazui S, Hajime T. DNA as a flocculation factor in Pseudomonas sp[J]. Agric. Biol. Chem., 1981 , 45 (12): 2869—2876
[85] Junji N, Shigeyoshi m, Yoshio H. Condition for production of microbial cell flocculant by Aspergillus sojae AJ7002[J]. Agric. Biol. Chem., 1976 , 40 (7): 1341—1347
[86]Salehizadeh H, Shojaosadati S A. Extracellular biopolymeric flocculants Recent trends and biotechnological importance[J]. Biotechnology Advances,2001,19:371—385
[87] 王泽民,王殿伟.造纸废水处理的研究[J].工业水处理,1994,14(2):16—19
[88] 吴福骞. 欧洲造纸废水零排放的进展[J].中华纸业,2004,25(4):56—59
[89] 卢学强. 造纸废水处理技术进展概述[J].四川环境,1998,17(2):25—28
[90] 刘成波,李发生,韩梅等. 紫外-可见分光光度法用于染料废水絮凝脱色效果测定的研究[J].中国环境监测,2001,17(3):22—24
[91] 沈永嘉. 有机颜料品种与应用[M]. 北京:化学工业出版社,2001.7
[92] 费华,张莉莎,周赛春. 丙烯酸类树脂为基料的水性油墨的研究[J]. 华中师范大学学报(自然科学版),2001(9):319—321
[93] 汤纯鹏,蔡凌,钮静,杨智淳. 颜料、油墨废水物化、生化法综合治理技术[J]. 城市环境与城市生态,1999(6):1—3
[94] 高华星,陆兴章,赵德仁. 阳离子高分子絮凝剂用于印刷油墨废水处理[J]. 环境污染与防治,1995(6):9—14
[2] 周群英,高延耀.环境工程微生物学[M].第二版.北京:高等教育出版社,2000,7
[3] 陆柱,蔡兰坤,陈中兴等.水处理药剂[M].北京:化学工业出版社,2002
[4] 崔子文,郝红英.水处理中絮凝剂的研究应用现状[J].山西化工,1999, 19 (1 ):58-61
[5] 马青山,贾瑟,孙丽珉等.絮凝化学和絮凝剂[M].第 1 版.北京:中国环境科学出版社,1990, 1-115
[6] American Water Works Association Coagulation Committee. Committee Report:coagulation as an integrated water treatment process. J Am Water Works Assoc[J], 1989,81(10):72
[7] 姚重华.混凝剂与絮凝剂[M].北京:中国环境科学出版社,1992.
[8] 李琼,肖锦,肖晶.接枝共聚型天然高分子絮凝剂的性能及应用研究[J].工业水处理,1997, 17 (4): 27-29
[9] 陶涛,卢秀清,冷静.微生物絮凝剂研究与应用进展[J].环境科学进展,1999(7): 21—25.
[10] 张育新,康勇.絮凝剂的研究现状及发展趋势[J].化工进展,2002, 21(11):799—804
[11] 常青.水处理絮凝学[M].北京:化学工业出版社,2003
[12] Dentel SK, Gossett JM. Mechanisms of coagulation with aluminums aits. J Am Water Works Assoc[J], 1988,80(4):187
[13] 吴健,戴桂馥.微生物细胞的絮凝与微生物絮凝剂[J].环境污染与防治,1994,16(6):27-32.
[14] 胡勇有,高健.微生物絮凝剂的研究与应用进展[J].环境科学进展,1999, 7(4):24-28
[15] 汤鸿宵.无机高分子复合絮凝剂的研制倾向[J].中国给水排水,1999, 15 (2):1-4
[16] 崔蕴霞,肖谨.铝盐絮凝剂及其环境效应[J].工业水处理,1998,18(3):6—9
[17] 吕新之.铝的污染和危害[J].环境保护,1998,5:38—39
[18] 金晓红,任华炜.环境生物技术应用与环境保护的新进展[J].环境保护,2002,2:18—19
[19] 郑怀礼等. 生物絮凝剂与絮凝技术[M]. 北京:化学工业出版社,2003. 11
[20] 马放,冯玉杰,任南琪等.环境生物技术[M]. 北京:化学工业出版社,2003.4
[21] 张彤,朱怀南,林哲.微生物絮凝剂的研究与应用进展[J]. 应用与环境生物学报,1996,(1):95—105
[22] 李桂娇,尹华,彭辉.生物絮凝剂的研究与开发[J]. 工业水处理,2002,22(3):9—12
[23] 陈坚,任洪强,堵国成等. 环境生物技术应用与发展[M].北京:中国轻工业出版社,2001
[24] Lee SH,Lee SO,Jang KL. Microbial flocculant from Arcuadendrom SP. TS-49[J]. Biotechnology Letters,1995,17(1):95—100
[25](日)有马启等.生物净化环境技术[M].化学工业出版社.北京,1990
[26] 马放,杨基先,金文标等编著.环境生物制剂的开发与应用[M].北京:化学工业出版社,2004.3
[27] 周少奇编著.环境生物技术[M].北京:科学出版社,2003.7
[28] Butterfield CT. Studies of sewage purification Ⅱ a Zooloea-forming bacterium isolated from activated sludge[J]. U.S.public Health rep, 1935,50:671
[29] Junji Nakamura. Purification and Chemical Analysis of Microbial Cell Flocculant produced by Aspergillus sojae AJ7002[J]. Agri. Biol. Chem,1976,40(3):619—624
[30] Takagi H, hadowaki K. Flocculant production by Paecilomyces sp. Taxonomic studies and culturecondition for production[J]. Agri. Biol. Chem, 1985,49(11):3151—3157
[31] Minoru Takeda, Ryuichiro Kurane. A Protien Bioflocculant Produced by Rhodococcus erythropolis[J].Agri.Biol.Chem,1951,55(10):2663—2664
[32] Suh H-H,et al. Characterication of bioflocculant produced by Bacillus sp. DP-152[J].Journal of fermentation and Bioengineering,1997,84(2): 108—112
[33] 王镇,王孔星. 几株絮凝剂产生菌的特性研究[J]. 微生物学报, 1995, 35 (2):121—129
[34] 马放,刘俊良,李淑更等.复合型微生物絮凝剂的开发[J].中国给水排水,2003,19(4):1—4
[35] 李剑,王曙光,高宝玉等.利用乳品废水生产微生物絮凝剂及其应用研究[J].环境工程,2004,22(6):93—94
[36] 王曙光,李剑,高宝玉等.一株产气肠杆菌絮凝特性的研究[J].环境化学,2005,24(2):171—174
[37] Kurane R, Takda K, Suzuki T. Screening for and characteristics of microbial flocculants[J]. Agric Biol Chem,1986,50(9):2301-2307
[38] Nakamura J, Miyashiro S, Hirose Y.Screening,isolation,and some properties of microbial cell flocculants[J]. Agric. Biol. Chem, 1976,40(2):377-383
[39] Sakka K, Takahashi H. Deoxyribonuclease-susceptible floc. forming Pseudomonas sp.[J] Agric Biol Chem,1981,45(12):2869-2876
[40] Takagi H, Kadowaki K. Purification and chemical properties of a flocculant produced by Paecilomyces[J]. Agric. Biol.Chem., 1985. 49( 11):3159-3164
[41] 周旭,黄丽萍,王竞等.利用鱼粉废水生产生物絮凝剂及其性能研究[J].应用与环境生物学报,2003, 9 (4): 436-438
[42] 石璐,刘忠义,唐受印等.微生物絮凝剂产生菌的筛选及培养条件优化[J].环境科学研究,2003, 16 (3): 35-36
[43] 邓述波,胡筱敏,罗茜.寄生曲霉产生絮凝剂的培养条件及其絮凝特性[J].应用与环境生物学报,1998, 4 (4) : 405-408
[44] 何宁,李寅,陆茂林等.营养和环境条件对生物絮凝剂合成的影响[J].应用与环境生物学报,2001, 7 (5): 483-488
[45] 秦培勇等.微生物絮凝剂及其絮凝机理的研究进展[J].内蒙古工业大学学报,2001, 20(3):166-170
[46] 刘紫鹃等.絮凝剂 BP25 的化学组成及结构研究[J].微生物学通报,2001,41 (3):348-352
[47] J.Nakaura,S.Miygeyshi,Y Hirose.Conditions for production of microbial cell flocculant by Aspergillus sojae AJ7002[J]. Agr.Biol.Chem.,1976 40 (7): 1341-1347
[48] 傅旭庆,汪大挥.微生物絮凝剂及其絮凝机理[J].污染防治技术,1998,11(1):6-9
[49] Zhang Tong, Lin Zhe. Microbial Flocculant and Its Application in Environmental Protection[J]. Environmental Sciences. 1999,(1): l—12
[50] 王猛,施宪法,柴晓利.微生物絮凝剂的研究与应用[J].化工环保,2001,21(6):328-332
[51] 翟广飞.产絮凝剂微生物的特征及其培养条件研究[D].武汉理工大学,2003,5
[52] 石璐. 高效微生物絮凝剂产生菌的筛选及培养条件研究[D].湘潭大学,2003,5
[53] 宫小燕,王曙光,栾兆坤等 微生物絮凝剂产生菌的筛选和优化以及在水处理中的应用[J]. 应用与环境生物学报,2003,9(2):196—199
[54] 程丽娟,薛泉宏主编.微生物学实验技术[M].世界图书出版公司,2004,4
[55] 石璐,刘忠义,唐受印等.微生物絮凝剂产生菌的筛选及培养条件优化[J].环境科学研究,2003,16(3):35—36,58
[56] Kurane R, Todea K, Takeda K.Culture conditions for production of microbial flocculant by Rhodococcus erythropolis[J]. Agri. Biol. Chem.,1986,50(9):2309—2313
[57] R.E.步坎南,N.E.吉本斯等. 伯杰细菌鉴定手册(第八版)[M]. 北京:科学出版社,1984,12
[58] Salehizadeh H, Shojaosadati S A. Extracellular biopolymeric flocculants Recent trends and biotechnological importance. Biotechnology Advances,2001,19:371~385
[59] Kurane R, Nohata Y. Microbial Flocculation of Waste Liquids and Oil Emulsion by a Bioflocculant from Alcaligenes latus. Agric Biol Chem,1991,44: 1127~1129
[60] Todea K, Kurane R. Microbial flocculant from Alcaligenes cuipdus KT201[J]. Agri. Biol. Chem.,1991,55(11):2793—2799
[61] Kazuki T, Kurane R. Microbial Flocculant from Alcaligenes cupidus KT201[J]. Agric Biol Chem,1991,55(11):2793—2799
[62] kakii K,Sugahara E,Shirakashi T. isolation and characterization of a Ca+-dependent floc-forming bacterium[J].Ferment.Technol.,1986,64(1):57—62
[63] Takakazu Endo, Kazuo Nakamura, Hajime Takahashi. Pronase—susceptible Floc Forming Bacteria : Relationship between Flocculation and Calcium Ion[J]. Agric Biol Chem, 1976,40 (11): 2289—2295
[64] Kurane R, Hatamochi K, Kakuno T. Purification and Characterization of Lipid Bioflocculant Produced by Rhodococcus erythropoils[J]. Biosci Biotech Biochem, 1994,58(11): 1977—1982
[65] 沈同,王镜岩.生物化学[M].第二版.北京
[66] Kazui S, Hajime T. DNA as a flocculation factor in Pseudomonas sp[J]. Agric. Biol. Chem., 1981 , 45 (12): 2869—2876
[67] Sousa M J, Texeira J A, Mota M. Difference in the flocculation mechanism of Kluyveromyces marxianus and Saccharomyces cerevisiae[J]. Biotechnology Letters, 1992, 14 (3): 213—218
[68] Salehizadeh H, Shojaosadati S A. Extracellular biopolymeric flocculants Recent trends and biotechnological importance[J]. Biotechnology Advances,2001,19:371—385
[69] 宫小燕,栾兆坤,王曙光等. 微生物絮凝剂絮凝特性的研究[J]. 环境化学,2001,20(6):550—556
[70] 李剑,冯贵颖,王曙光等. 絮凝剂产生菌 KLE—1 的特性研究[J]. 西北农林科技大学学报,2004,32(增刊):121—124
[71] Tezuka Y. Cation—dependent flocculation in a flavobacterium species predominant in activated sludge[J]. Applied Microbiology, 1969, 17 (2): 222—226
[72] Endo T,Nakumaru K,Takahashi H. Pronase-susceptible floc forming bacteria:relationship between flocculation and calcium ion. Agri.Biol.Chem.,1976,40(11):2289—2295
[73] Sousa M J, Texeira J A, Mota M. Difference in the flocculation mechanism of Kluyveromyces marxianus and Saccharomyces cerevisiae[J]. Biotechnology Letters, 1992, 14 (3): 213—218
[74] Bar-or Y and Shilo M. Chrarcterization of macromolecular flocculants produced by Phormidium sp. Strain J-1 and by Anabaenopsis circularis PCC6720[J].Applied and Environmental Microbiology,1987,53(9):2226—2230
[75] Choi CW,Yoo SA,Oh IH,Park SH. Characterization of an extracellular flocculating substance produced by a planktonic cyanobacterium, Anabaena sp.[J], Biotechnology Letters,1998,20(7):643—646
[76] 刘春. 皂素生产废水污染特点及治理对策探讨[J]. 环境保护科学,2001,27(6):22—24
[77] 单丽伟,冯贵颖,朱丹等. 上流式厌氧污泥床(UASB)处理皂素废水的研究[J]. 西北农林科技大学学报,2003,31:109—112
[78] 朱丹,冯贵颖,呼世斌等. 皂素废水处理中厌氧污泥活性影响因素的研究[J]. 西北农林科技大学学报,2005,33(12):103—106
[79] 周德庆. 微生物学教程. 第一版. 北京:高等教育出版社,1995
[80] 孔繁翎. 环境生物学. 北京:高等教育出版社,2002,5
[81] Ning He, Yin Li, Jian Chen, Shi-Yi. Identification of a novel bioflocculant from a newly isolated Coynebacterium glutamicum[J]. Biochemical Engineering Journal,2002,11:137—148
[82] Kurane R, Hatamochi K. Chemical structure of lipid bioflocculant produced by Rhodococcus erythropolis[J]. Agri.Biol.Chem.,1995,59(9):1652—1656
[83] 袁志发,周静芋. 试验设计与分析[M].北京,高等教育出版社,2000,8
[84] Kazui S, Hajime T. DNA as a flocculation factor in Pseudomonas sp[J]. Agric. Biol. Chem., 1981 , 45 (12): 2869—2876
[85] Junji N, Shigeyoshi m, Yoshio H. Condition for production of microbial cell flocculant by Aspergillus sojae AJ7002[J]. Agric. Biol. Chem., 1976 , 40 (7): 1341—1347
[86]Salehizadeh H, Shojaosadati S A. Extracellular biopolymeric flocculants Recent trends and biotechnological importance[J]. Biotechnology Advances,2001,19:371—385
[87] 王泽民,王殿伟.造纸废水处理的研究[J].工业水处理,1994,14(2):16—19
[88] 吴福骞. 欧洲造纸废水零排放的进展[J].中华纸业,2004,25(4):56—59
[89] 卢学强. 造纸废水处理技术进展概述[J].四川环境,1998,17(2):25—28
[90] 刘成波,李发生,韩梅等. 紫外-可见分光光度法用于染料废水絮凝脱色效果测定的研究[J].中国环境监测,2001,17(3):22—24
[91] 沈永嘉. 有机颜料品种与应用[M]. 北京:化学工业出版社,2001.7
[92] 费华,张莉莎,周赛春. 丙烯酸类树脂为基料的水性油墨的研究[J]. 华中师范大学学报(自然科学版),2001(9):319—321
[93] 汤纯鹏,蔡凌,钮静,杨智淳. 颜料、油墨废水物化、生化法综合治理技术[J]. 城市环境与城市生态,1999(6):1—3
[94] 高华星,陆兴章,赵德仁. 阳离子高分子絮凝剂用于印刷油墨废水处理[J]. 环境污染与防治,1995(6):9—14