艾灸对DPN模型大鼠神经生长因子的影响研究
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摘要
目的：研究艾灸对糖尿病周围神经病变(DPN)模型大鼠坐骨神经神经生长因子(NGF)的影响，从神经保护的角度探讨艾灸治疗DPN的部分作用机制。
方法：以链脲佐菌素腹腔注射诱导形成的DPN模型大鼠作为研究对象，根据“益气通络解毒”的治疗原则，艾灸“胰俞”、“足三里”穴，每日1次，每穴15分钟，连续治疗56天。通过监测血糖、尿糖、体重、饮食量等一般情况变化，结合神经电生理方法检测神经传导速度，评价艾灸对DPN治疗效应；HE染色、光镜观察坐骨神经组织结构变化；免疫组化检测坐骨神经NGF表达；酶联免疫吸附法检测坐骨神经NGF含量。
结果：治疗后，艾灸组大鼠血糖水平明显低于模型组(P<0.01)；艾灸组感觉神经传导速度明显快于模型组(P<0.01)。艾灸组病理形态改变较模型组有明显改善。模型组大鼠坐骨神经NGF阳性表达较弱，艾灸组明显增强，阳性表达面积、积分光密度、平均灰度值比较，P<0.01；艾灸组NGF含量也明显高于模型组(P<0.01)。
结论1．艾灸能有效改善DPN模型大鼠周围神经病变症状、体征。2．艾灸改善DPN模型大鼠周围神经症状的效应可能与艾灸促进NGF表达、提高NGF含量，促进神经保护有关。
Objective: To study the influence of moxibustion therapy on diabetic peripheral neuropathy (DPN) rats sciatic nerve growth factor (NGF) and provide experimental evidence for neuroprotect mechanism on DPN treated by moxibustion. Methods: The DPN rats induced by i.p. Streptozotocin were treated by moxibustion on " Yishu ( EX-B3)" and "Zusanli ( ST36 ) " acupoints according to "Yi Qi Tong Luo Jie Du" principle. The treatment was once daily and lasted for 56 days. We detected the changes of blood sugar, urine sugar, quantity of drinking and eating. We used nerve electrophysiological method to test the nerve conduction velocity (NCV) changes. We observed the morphological changes of sciatic nerve by optic microscope after HE stained and analyzed quantititily the positive expression of NGF on DPN rats sciatic nerve after immunohistochemistry stained. In addition, we measured the concentration of NGF by enzyme-linked immuno sorbent assay (ELISA) . Results: 1.Comparing with model group,the blood sugar level of moxibustion group are obviously low (P<0.01), the NCV are obviously quick (P<0.01), and the morphological changes of sciatic nerve get obviously improved. 2. Comparing with model group,the expression level of NGF in the sciatic nerve of moxibustion-treated group are significantly high (P<0.01), and the concentration of NGF in sciatic nerve of moxibustion-treated group are obviously more (P<0.01) . Conclusion: Moxibustion therapy can improve symptoms and sign of DPN. The mechanism may be related to enhance the expression and increase the concentration of NGF to protect DPN nerve.
方法：以链脲佐菌素腹腔注射诱导形成的DPN模型大鼠作为研究对象，根据“益气通络解毒”的治疗原则，艾灸“胰俞”、“足三里”穴，每日1次，每穴15分钟，连续治疗56天。通过监测血糖、尿糖、体重、饮食量等一般情况变化，结合神经电生理方法检测神经传导速度，评价艾灸对DPN治疗效应；HE染色、光镜观察坐骨神经组织结构变化；免疫组化检测坐骨神经NGF表达；酶联免疫吸附法检测坐骨神经NGF含量。
结果：治疗后，艾灸组大鼠血糖水平明显低于模型组(P<0.01)；艾灸组感觉神经传导速度明显快于模型组(P<0.01)。艾灸组病理形态改变较模型组有明显改善。模型组大鼠坐骨神经NGF阳性表达较弱，艾灸组明显增强，阳性表达面积、积分光密度、平均灰度值比较，P<0.01；艾灸组NGF含量也明显高于模型组(P<0.01)。
结论1．艾灸能有效改善DPN模型大鼠周围神经病变症状、体征。2．艾灸改善DPN模型大鼠周围神经症状的效应可能与艾灸促进NGF表达、提高NGF含量，促进神经保护有关。
Objective: To study the influence of moxibustion therapy on diabetic peripheral neuropathy (DPN) rats sciatic nerve growth factor (NGF) and provide experimental evidence for neuroprotect mechanism on DPN treated by moxibustion. Methods: The DPN rats induced by i.p. Streptozotocin were treated by moxibustion on " Yishu ( EX-B3)" and "Zusanli ( ST36 ) " acupoints according to "Yi Qi Tong Luo Jie Du" principle. The treatment was once daily and lasted for 56 days. We detected the changes of blood sugar, urine sugar, quantity of drinking and eating. We used nerve electrophysiological method to test the nerve conduction velocity (NCV) changes. We observed the morphological changes of sciatic nerve by optic microscope after HE stained and analyzed quantititily the positive expression of NGF on DPN rats sciatic nerve after immunohistochemistry stained. In addition, we measured the concentration of NGF by enzyme-linked immuno sorbent assay (ELISA) . Results: 1.Comparing with model group,the blood sugar level of moxibustion group are obviously low (P<0.01), the NCV are obviously quick (P<0.01), and the morphological changes of sciatic nerve get obviously improved. 2. Comparing with model group,the expression level of NGF in the sciatic nerve of moxibustion-treated group are significantly high (P<0.01), and the concentration of NGF in sciatic nerve of moxibustion-treated group are obviously more (P<0.01) . Conclusion: Moxibustion therapy can improve symptoms and sign of DPN. The mechanism may be related to enhance the expression and increase the concentration of NGF to protect DPN nerve.
引文
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1．蒋雨平编．临床神经病学．上海医科大学出版社[M]．1999，370-378
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