两个TRP离子通道受体在黑腹果蝇免疫反应中的初步研究
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摘要
先天性免疫是机体抵抗一切病原体的第一道防线。在过去的二十年中,黑腹果蝇(Drosophila melanogaster)作为研究先天性免疫的一种特别有用的模型生物,从遗传学的角度详细剖析了先天性免疫的各个层面。由这些研究而来的诸多认识直接导致了哺乳动物体系的一些受体如Toll-like受体,及其信号通路的重大发现。先天性免疫在从昆虫到哺乳动物进化上的保守已经被证实。我的研究工作主要围绕黑腹果蝇的两种瞬时受体电位通道受体(TRP channelreceptor):CG31284和CG34123。在论文里,我通过P因子介导的不精确剪切获得这两个TRP基因各自的果蝇突变体,其中,一个是CG31284基因的功能增益突变,另一个则为CG34123的功能缺失突变。我们用一些病原体感染这些果蝇突变体,发现这两个突变体有不同的免疫效应:CG31284表达量增多的果蝇对这类感染更有抵抗力,而CG34123表达量减少的果蝇对这类感染表现的比较敏感。以上结果表明:TRP通道受体在果蝇的免疫反应中扮演着一定的角色。
Innate immunity is the first line to defense host against all kinds of pathogens.In last two decades,Drosophila melanogaster has served as a particularly useful model Organism for studying and genetically dissecting the aspect of innate immunity.The immense knowledge derived from such studies has led to the important discovery of the receptors like the toll like receptors and their signalling pathways in mammalian systems.Innate immunity has been shown to be evolutionarily conserved from insect to mammals.My research work is focused on two transient receptor potential(TRP) channel receptors in Drosophila melanogaster:CG31284 and CG34123.In my thesis, mutant flies of the two TRP genes have been generated by P-element mediated imprecise excision.One gain of function mutation for CG31284 and one loss of function mutation for CG34123 were produced.We infected these mutant flies with a number of pathogens and the two alleles showed different effects to infection.The flies with increased expression of CG31284 were more resistant to infection,while those with decreased expression of CG34123 were more susceptible to infection. These results indicate that TRP receptors have a role in Drosophila immune responses.
Innate immunity is the first line to defense host against all kinds of pathogens.In last two decades,Drosophila melanogaster has served as a particularly useful model Organism for studying and genetically dissecting the aspect of innate immunity.The immense knowledge derived from such studies has led to the important discovery of the receptors like the toll like receptors and their signalling pathways in mammalian systems.Innate immunity has been shown to be evolutionarily conserved from insect to mammals.My research work is focused on two transient receptor potential(TRP) channel receptors in Drosophila melanogaster:CG31284 and CG34123.In my thesis, mutant flies of the two TRP genes have been generated by P-element mediated imprecise excision.One gain of function mutation for CG31284 and one loss of function mutation for CG34123 were produced.We infected these mutant flies with a number of pathogens and the two alleles showed different effects to infection.The flies with increased expression of CG31284 were more resistant to infection,while those with decreased expression of CG34123 were more susceptible to infection. These results indicate that TRP receptors have a role in Drosophila immune responses.
引文
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