红掌离体培养再生体系的建立
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摘要
本文主要以红掌的叶片作为外植体进行了离体培养的研究。在红掌离体培养技术研究过程中,通过对红掌外植体的选择,愈伤组织的诱导,不定芽的诱导和增殖,以及红掌组培苗根系诱导的最佳条件进行了筛选,最终建立了红掌离体培养再生体系。主要研究结果如下:
1、红掌外植体的选择。主要是对红掌不同的外植体进行消毒效果的研究。结果表明:使用2.5%次氯酸钠或0.1%升汞进行消毒,叶片和佛焰苞片的消毒效果最好,初展叶片和初展佛焰苞片污染率为0.0%;叶柄、花柄和茎段的消毒效果最差,最高污染率达到97.5%。在叶片和佛焰苞消毒中,使用0.1% HgCl2 8min进行消毒,叶片和佛焰苞片消毒效果最好。
2、红掌愈伤组织的诱导。主要以叶片为外植体,进行愈伤组织的诱导。探讨不同激素、不同激素组合、蔗糖处理等对愈伤组织诱导的影响。结果表明:最适宜的培养基为MS+6-BA 1.0 mg/L+2,4-D 0.1 mg/L+KT 0.05mg/L,初展叶片在这种培养基中进行暗培养,愈伤组织诱导率最高为86.67%,且愈伤组织生长良好。
3、红掌不定芽的诱导。主要从不同激素、不同激素组合、蔗糖处理等对红掌不定芽的诱导影响。结果表明:考虑到不定芽的质量和数量,在改良MS+6-BA 1.0mg/L+IBA 0.3mg/L的培养基中进行光照培养,对红掌不定芽的诱导效果最好,不定芽的诱导率高为87.5%,平均诱导芽数为5.8±1.08个。
4、红掌不定芽复壮培养。结果表明:最适宜的壮苗培养基为MS+6-BA 1.0mg/L+IBA 0.3mg/L,不定芽在这种培养基中生长最快,45天后平均株高可达到5.59㎝。对不定芽进行复壮时,发现在原愈伤组织诱导培养基中不定芽生长状况良好。
5、红掌组培苗根系的诱导。主要探讨不同激素对不定芽生根的影响。把不定芽接入生根培养基中进行生根。结果表明:红掌最适宜的培养基为1/2MS+IBA 0.7+NAA 0.3(mg/L)。在这种培养基中,可有效的促进不定芽提早生根,根系比较粗壮,根毛较多,植株生长旺盛。
1. Anthurium explants choice. Take the explants choice of anthurium andraeanum. Disscuessed disinfection effect of the different explants . The results indicated that: we taked 2.5% of Sodium hypochlorite and 0.1%of Mercuric chloride, Anthurium leaves and Petales were disinfected, this effect was very good. Rate of contamination of leaves and Petales reached 0.0%;This stems of leaf, petal and stems were disinfected ,which effect was worst, and rate of contamination reached 97.5%.In the leaves and Petales disinfection, we taked 0.1% HgCl2 8 minutes for disinfection, we finded disinfectan effect of leaves and Petales are best in all explants.
2. Anthurium Callus induction.Take the leaf of anthurium andraeanum as explants, which inducted Callus of anthurium andraeanum. Discussed the different hormone, the different hormone combination, sucrose density processing and so on had influence of Callus induction .The results indicated that: the most suitable callus induction culture medium was MS+6-BA 1.0+2,4-D 0.1+KT 0.05 in dark, the leaf was cultured in this medium, The highest rate of Callus induction reached to 86.67%,and Callus growed well.
3. Anthurium adventitious buds induction. Discussed the different hormone, the different hormone combination, sucrose density processing and so on had influence of anthurium adventitious buds induction . The results inducted that: in improvement Medium, taked MS + 6-BA1.0 + IBA0.3 (mg/L) in Lighting, The induction of anthurium adventitious buds growed well,and the rate of adventitious buds induction reached to 87.5%,and the average number of buds rached to5.8±1.08.
4. Anthurium adventitious buds culture of growth. The results inducted that: The most suitable growth culture medium of adventitious buds was MS + 6-BA1.0 +IBA0.3(mg/L).in this medium, adventitious buds growed very fast,and the average height reached to 5.59 cm after 45 days. in the growth medium of adventitious buds, we found that adventitious buds growed well in the original callus induction medium.
5. Induction of Adventitious buds roots. Main discussed the different hormone, the different hormone combination, had influence of roots of anthurium adventitious .we would induct adventitious buds to carry on the root formation medium.The results inducted that: the root of adventitious buds was suitable the induction culture medium which was 1/2MS+IBA0.7+NAA0.3(mg/L). Induction of roots was growed very early and fast in the Anthurium. It was effective that promoted early roots in this medium, comparison sturdy roots; more roots hair and vigorous plants growth.
1、红掌外植体的选择。主要是对红掌不同的外植体进行消毒效果的研究。结果表明:使用2.5%次氯酸钠或0.1%升汞进行消毒,叶片和佛焰苞片的消毒效果最好,初展叶片和初展佛焰苞片污染率为0.0%;叶柄、花柄和茎段的消毒效果最差,最高污染率达到97.5%。在叶片和佛焰苞消毒中,使用0.1% HgCl2 8min进行消毒,叶片和佛焰苞片消毒效果最好。
2、红掌愈伤组织的诱导。主要以叶片为外植体,进行愈伤组织的诱导。探讨不同激素、不同激素组合、蔗糖处理等对愈伤组织诱导的影响。结果表明:最适宜的培养基为MS+6-BA 1.0 mg/L+2,4-D 0.1 mg/L+KT 0.05mg/L,初展叶片在这种培养基中进行暗培养,愈伤组织诱导率最高为86.67%,且愈伤组织生长良好。
3、红掌不定芽的诱导。主要从不同激素、不同激素组合、蔗糖处理等对红掌不定芽的诱导影响。结果表明:考虑到不定芽的质量和数量,在改良MS+6-BA 1.0mg/L+IBA 0.3mg/L的培养基中进行光照培养,对红掌不定芽的诱导效果最好,不定芽的诱导率高为87.5%,平均诱导芽数为5.8±1.08个。
4、红掌不定芽复壮培养。结果表明:最适宜的壮苗培养基为MS+6-BA 1.0mg/L+IBA 0.3mg/L,不定芽在这种培养基中生长最快,45天后平均株高可达到5.59㎝。对不定芽进行复壮时,发现在原愈伤组织诱导培养基中不定芽生长状况良好。
5、红掌组培苗根系的诱导。主要探讨不同激素对不定芽生根的影响。把不定芽接入生根培养基中进行生根。结果表明:红掌最适宜的培养基为1/2MS+IBA 0.7+NAA 0.3(mg/L)。在这种培养基中,可有效的促进不定芽提早生根,根系比较粗壮,根毛较多,植株生长旺盛。
1. Anthurium explants choice. Take the explants choice of anthurium andraeanum. Disscuessed disinfection effect of the different explants . The results indicated that: we taked 2.5% of Sodium hypochlorite and 0.1%of Mercuric chloride, Anthurium leaves and Petales were disinfected, this effect was very good. Rate of contamination of leaves and Petales reached 0.0%;This stems of leaf, petal and stems were disinfected ,which effect was worst, and rate of contamination reached 97.5%.In the leaves and Petales disinfection, we taked 0.1% HgCl2 8 minutes for disinfection, we finded disinfectan effect of leaves and Petales are best in all explants.
2. Anthurium Callus induction.Take the leaf of anthurium andraeanum as explants, which inducted Callus of anthurium andraeanum. Discussed the different hormone, the different hormone combination, sucrose density processing and so on had influence of Callus induction .The results indicated that: the most suitable callus induction culture medium was MS+6-BA 1.0+2,4-D 0.1+KT 0.05 in dark, the leaf was cultured in this medium, The highest rate of Callus induction reached to 86.67%,and Callus growed well.
3. Anthurium adventitious buds induction. Discussed the different hormone, the different hormone combination, sucrose density processing and so on had influence of anthurium adventitious buds induction . The results inducted that: in improvement Medium, taked MS + 6-BA1.0 + IBA0.3 (mg/L) in Lighting, The induction of anthurium adventitious buds growed well,and the rate of adventitious buds induction reached to 87.5%,and the average number of buds rached to5.8±1.08.
4. Anthurium adventitious buds culture of growth. The results inducted that: The most suitable growth culture medium of adventitious buds was MS + 6-BA1.0 +IBA0.3(mg/L).in this medium, adventitious buds growed very fast,and the average height reached to 5.59 cm after 45 days. in the growth medium of adventitious buds, we found that adventitious buds growed well in the original callus induction medium.
5. Induction of Adventitious buds roots. Main discussed the different hormone, the different hormone combination, had influence of roots of anthurium adventitious .we would induct adventitious buds to carry on the root formation medium.The results inducted that: the root of adventitious buds was suitable the induction culture medium which was 1/2MS+IBA0.7+NAA0.3(mg/L). Induction of roots was growed very early and fast in the Anthurium. It was effective that promoted early roots in this medium, comparison sturdy roots; more roots hair and vigorous plants growth.
引文
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