IGF-2与VEGF在翼状胬肉中的表达及意义
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摘要
目的：
本实验通过对胰岛素样生长因子2(IGF-2)与血管内皮生长因子(VEGF)在翼状胬肉中表达情况的研究,进一步探讨二者之间的相关性,为深入地研究和积极治疗翼状胬肉提供理论依据,并且为术前用药、术后防止复发奠定基础。方法：
研究对象均来自2009年11月至2010年10月期间在延边大学附属医院手术治疗的翼状胬肉患者及斜视患者共52例。实验前均排除其他眼部疾病。
实验组：选取胬肉患者翼状胬肉标本42例,患者年龄在38岁至65岁之间,均单眼发病(男性19例,女性23例)。按病变组织有无充血、肥厚将翼状胬肉分为两组：(1)静止期组17例,(2)进行期组25例。
对照组：选择斜视患者正常结膜标本10例(均为球结膜),患者年龄在32岁至58岁之间(男性6例,女性4例)。
采用免疫组化SABC法分别检测IGF-2与VEGF在两组之间的表达情况。
结果：
1.IGF-2在翼状胬肉进行期组、翼状胬肉静止期组、正常结膜组的阳性表达率分别为80.0%、41.2%、0.0%,进行期组分别与静止期组、正常结膜组相比较,差异有统计学意义(P<0.05);翼状胬肉静止期组与正常结膜组相比较,差异无统计学意义(P>0.05)。
2. VEGF在翼状胬肉进行期组、翼状胬肉静止期组、正常结膜组的阳性表达率分别为92.0%、52.9%、30.0%,进行期组分别与静止期组、正常结膜组相比较,差异有统计学意义(P<0.05);翼状胬肉静止期组与正常结膜组相比较,差异无统计学意义(P>0.05)。
3.用等级资料的相关性分析检验在翼状胬肉中IGF-2与VEGF两种因子阳性表达的相关性,结果显示二项指标的表达结果相互间呈正相关(r=0.378,P<0.05)。结论：
1.IGF-2与VEGF在翼状胬肉进行期、静止期中的表达均高于正常结膜；
2.IGF-2与VEGF可能促使翼状胬肉的发生、发展；
3.IGF-2与VEGF在翼状胬肉的发病机制中可能具有协同作用。
Objective:
The objective is to provide the theory basis for pathological mechanism, premedicant and prevent the recurrence of Pterygium through evaluate the expression level and relationship of IGF-2, VEGF in pterygium.
Methods:
52 cases patients with pterygium and strabismus were selected, all the patients were surgeried in yanbian university hospital from 2009.11 to 2010.10,28. All the cases were excluded other diseases of eye before surgery. All the cases were invaded into two groups.
Experiment group:42 cases of pterygium, Age from38 to 65 and all the cases were with unilateral eye diseases(male:19, female:23). According to the the degree of local congestion and hypertrophy all patients were also divided into two groups: (1)Static group consisting of 17 patients; (2)Progressive group consisting of 25 patients.
Control group:10 cases of patients with strabismus were selected as control group, Age from32 to 58(male:6, female:4).
Evaluating the expression level of IGF-2 and VEGF in the two groups by immunohistochemistry.
Results:
1. The positive expression rate of IGF-2 in static group, progressive group and control group was 80.0%,41.2%vand 0.0% respectively, There were significant differences between the pterygium group and static group, control group respectively (P<0.05); There was no differences between the static group and control group in pterygium (P>0.05).
2. The positive expression rate of VEGF in static group, progressive group and control group was 92.0%,52.9%vand30.0% respectively, There were significant differences between the pterygium group and static group, control group respectively (P<0.05); There was no differences between the static group and control group in pterygium (P>0.05).
3. The correlation of the positive express between IGF-2 and VEGF was analyzed by Spearman rank correlation method. The results showed that there was significantly positive correlation between IGF-2 and VEGF (r=0.378, P<0.05)
Conclusion:
1. The expression of IGF-2 and VEGF were higher than control group in the progressive and static stage of pterygium;
2. IGF-2 and VEGF may contribute to the occurrence and development of pterygium;
3. IGF-2 and VEGF in pterygium may have synergistic on pathogenesis.
本实验通过对胰岛素样生长因子2(IGF-2)与血管内皮生长因子(VEGF)在翼状胬肉中表达情况的研究,进一步探讨二者之间的相关性,为深入地研究和积极治疗翼状胬肉提供理论依据,并且为术前用药、术后防止复发奠定基础。方法：
研究对象均来自2009年11月至2010年10月期间在延边大学附属医院手术治疗的翼状胬肉患者及斜视患者共52例。实验前均排除其他眼部疾病。
实验组：选取胬肉患者翼状胬肉标本42例,患者年龄在38岁至65岁之间,均单眼发病(男性19例,女性23例)。按病变组织有无充血、肥厚将翼状胬肉分为两组：(1)静止期组17例,(2)进行期组25例。
对照组：选择斜视患者正常结膜标本10例(均为球结膜),患者年龄在32岁至58岁之间(男性6例,女性4例)。
采用免疫组化SABC法分别检测IGF-2与VEGF在两组之间的表达情况。
结果：
1.IGF-2在翼状胬肉进行期组、翼状胬肉静止期组、正常结膜组的阳性表达率分别为80.0%、41.2%、0.0%,进行期组分别与静止期组、正常结膜组相比较,差异有统计学意义(P<0.05);翼状胬肉静止期组与正常结膜组相比较,差异无统计学意义(P>0.05)。
2. VEGF在翼状胬肉进行期组、翼状胬肉静止期组、正常结膜组的阳性表达率分别为92.0%、52.9%、30.0%,进行期组分别与静止期组、正常结膜组相比较,差异有统计学意义(P<0.05);翼状胬肉静止期组与正常结膜组相比较,差异无统计学意义(P>0.05)。
3.用等级资料的相关性分析检验在翼状胬肉中IGF-2与VEGF两种因子阳性表达的相关性,结果显示二项指标的表达结果相互间呈正相关(r=0.378,P<0.05)。结论：
1.IGF-2与VEGF在翼状胬肉进行期、静止期中的表达均高于正常结膜；
2.IGF-2与VEGF可能促使翼状胬肉的发生、发展；
3.IGF-2与VEGF在翼状胬肉的发病机制中可能具有协同作用。
Objective:
The objective is to provide the theory basis for pathological mechanism, premedicant and prevent the recurrence of Pterygium through evaluate the expression level and relationship of IGF-2, VEGF in pterygium.
Methods:
52 cases patients with pterygium and strabismus were selected, all the patients were surgeried in yanbian university hospital from 2009.11 to 2010.10,28. All the cases were excluded other diseases of eye before surgery. All the cases were invaded into two groups.
Experiment group:42 cases of pterygium, Age from38 to 65 and all the cases were with unilateral eye diseases(male:19, female:23). According to the the degree of local congestion and hypertrophy all patients were also divided into two groups: (1)Static group consisting of 17 patients; (2)Progressive group consisting of 25 patients.
Control group:10 cases of patients with strabismus were selected as control group, Age from32 to 58(male:6, female:4).
Evaluating the expression level of IGF-2 and VEGF in the two groups by immunohistochemistry.
Results:
1. The positive expression rate of IGF-2 in static group, progressive group and control group was 80.0%,41.2%vand 0.0% respectively, There were significant differences between the pterygium group and static group, control group respectively (P<0.05); There was no differences between the static group and control group in pterygium (P>0.05).
2. The positive expression rate of VEGF in static group, progressive group and control group was 92.0%,52.9%vand30.0% respectively, There were significant differences between the pterygium group and static group, control group respectively (P<0.05); There was no differences between the static group and control group in pterygium (P>0.05).
3. The correlation of the positive express between IGF-2 and VEGF was analyzed by Spearman rank correlation method. The results showed that there was significantly positive correlation between IGF-2 and VEGF (r=0.378, P<0.05)
Conclusion:
1. The expression of IGF-2 and VEGF were higher than control group in the progressive and static stage of pterygium;
2. IGF-2 and VEGF may contribute to the occurrence and development of pterygium;
3. IGF-2 and VEGF in pterygium may have synergistic on pathogenesis.
引文
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