透明质酸相关微纳结构的制备/表征及其对细胞/细菌的影响
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摘要
心血管植入材料必须具有良好的血液相容性,材料表面内皮化是提高材料血液相容性的理想方法。本文中,利用毛细力刻蚀法在碱活化钛表面制备透明质酸(HA)微图形,采用扫描电子显微镜(SEM),光镜,傅里叶红外变换光谱分析(FTIR)、接触角测量仪、表面轮廓仪对样品性进行了表征。光镜和表面轮廓仪检测结果表明：采用浇铸法可以制备出沟、脊分明、表面平整的PDMS弹性印章；SEM、FTIR和接触角测量结果表明：碱活化后钛表面形貌和粗糙度发生改变,出现-OH伸缩振动峰,其亲水性明显提高；光镜和轮廓仪检测结果表明：采用软刻蚀(微转移模塑)的方法在碱活化钛表面获得了轮廓清晰的透明质酸条纹状微图形。在透明质酸微图形表面进行平滑肌细胞和内皮细胞的共培养,使内皮细胞的形态和分布类似体内血管内皮细胞。
生物材料相关感染是临床使用生物材料时伴随的一个严重问题。至今已有多种抗菌生物材料被用来防治植入物引起的感染。纳米银是一种安全、高效的新型抗菌剂。将纳米银应用于植入物中使其自身具有抗菌的功能。以透明质酸为分散剂,通过紫外光还原硝酸银制备纳米银颗粒。用紫外-可见光分光光度计(UV-Vis)、扫描电子显微镜、X射线光谱仪(EDX)、动态激光光散射粒度仪(DLS)、Zeta电位仪及细菌试验和细胞培养实验表征纳米银的形貌和性质。结果表明,所制备的纳米银呈球形,平均粒径为170nm,对大肠杆菌有抑菌作用,对细胞的作用有待进一步研究。
Implanting cardiovascular materials must be provided with favorable hemocompatibility. Endothelialization on the surface of the implanting materials is always one of the ideal method to improve the hemocompatibility. In this thesis, capillary force lithography is used to fabricate the hyaluronic acid (HA) micropattern on the activated titanium (Ti-OH) surface. The HA micropatterns were characterized by scanning electron microscope(SEM), light microscopy, fourier transform infrared spectroscopy(FTIR), contact angle measurement, surface profiler. Surface profiler and light microscopy results showed that polydimethyl-siloxane (PDMS) stamp with high fidelity. The results of SEM, FTIR and contact angle measurement showed that the morphology of Ti surface activated by NaOH solution were changed, its roughness and hydrophilicity increased and ophilic groups was detected on the surface. Light microscopy and surface profiler results demonstrated that HA micropatterns had been prepared accurately on the surface of Ti-OH. Co-culture of human umbilical vein endothelial cells with human umbilical artery smooth muscle cells on the HA micropattern. The morphology and distribution of the co-culture Ecs is more similar to that in vivo.
Biomaterial centered infections remain the major and often irreducible complication in the clinical use of biomaterials. To date, many kinds of antibacterial biomaterials have been studied for the prevention or treatment of bacterial infection on implanted devices. The nano-silver is a safe, highly effective new antimicrobial agents. Nano-silver is applied to the hunman implants to make sure that the implanted devices themselves possess antimicrobial function. HA was used as the stabilizing agents and the templates to prepare metal nanoparticles by photo-reduction of silver nitrate. The properties of the silver nanoparticles were investigated by UV-Vis spectroscopy, scanning electron microscope(SEM), Energy Dispersive X-Ray Spectroscopy (EDX), dynamic light scattering (DLS), Zeta potential instrument, antim icrobial activity test and cell culture experiments. Results show that the obtained silver nanoparticles are spherical and the average diameter is about170nm. Furthermore, the silver nanoparticles exhibit antibacter ialproperties against Escherichi a coli. However, the cytotoxieity of silver nanoparticles is to be studied further more.
生物材料相关感染是临床使用生物材料时伴随的一个严重问题。至今已有多种抗菌生物材料被用来防治植入物引起的感染。纳米银是一种安全、高效的新型抗菌剂。将纳米银应用于植入物中使其自身具有抗菌的功能。以透明质酸为分散剂,通过紫外光还原硝酸银制备纳米银颗粒。用紫外-可见光分光光度计(UV-Vis)、扫描电子显微镜、X射线光谱仪(EDX)、动态激光光散射粒度仪(DLS)、Zeta电位仪及细菌试验和细胞培养实验表征纳米银的形貌和性质。结果表明,所制备的纳米银呈球形,平均粒径为170nm,对大肠杆菌有抑菌作用,对细胞的作用有待进一步研究。
Implanting cardiovascular materials must be provided with favorable hemocompatibility. Endothelialization on the surface of the implanting materials is always one of the ideal method to improve the hemocompatibility. In this thesis, capillary force lithography is used to fabricate the hyaluronic acid (HA) micropattern on the activated titanium (Ti-OH) surface. The HA micropatterns were characterized by scanning electron microscope(SEM), light microscopy, fourier transform infrared spectroscopy(FTIR), contact angle measurement, surface profiler. Surface profiler and light microscopy results showed that polydimethyl-siloxane (PDMS) stamp with high fidelity. The results of SEM, FTIR and contact angle measurement showed that the morphology of Ti surface activated by NaOH solution were changed, its roughness and hydrophilicity increased and ophilic groups was detected on the surface. Light microscopy and surface profiler results demonstrated that HA micropatterns had been prepared accurately on the surface of Ti-OH. Co-culture of human umbilical vein endothelial cells with human umbilical artery smooth muscle cells on the HA micropattern. The morphology and distribution of the co-culture Ecs is more similar to that in vivo.
Biomaterial centered infections remain the major and often irreducible complication in the clinical use of biomaterials. To date, many kinds of antibacterial biomaterials have been studied for the prevention or treatment of bacterial infection on implanted devices. The nano-silver is a safe, highly effective new antimicrobial agents. Nano-silver is applied to the hunman implants to make sure that the implanted devices themselves possess antimicrobial function. HA was used as the stabilizing agents and the templates to prepare metal nanoparticles by photo-reduction of silver nitrate. The properties of the silver nanoparticles were investigated by UV-Vis spectroscopy, scanning electron microscope(SEM), Energy Dispersive X-Ray Spectroscopy (EDX), dynamic light scattering (DLS), Zeta potential instrument, antim icrobial activity test and cell culture experiments. Results show that the obtained silver nanoparticles are spherical and the average diameter is about170nm. Furthermore, the silver nanoparticles exhibit antibacter ialproperties against Escherichi a coli. However, the cytotoxieity of silver nanoparticles is to be studied further more.
引文
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