碱性染料间能量转移荧光分析的研究
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摘要
本文共分五章。第一章为文献综述，对荧光共振能量转移技术的原理、分析
技术及其应用进行了较全面的综述。引用文献56篇。
第二章对碱性荧光染料吖啶橙（AO）、吖啶黄（AY）、罗丹明6G（R6G）、罗丹明
B（RB）、乙基罗丹明B（ERB）、丁基罗丹明B（BRB）、碱性藏花红（ST）组成的各
种染料混合体系的能量转移进行了研究。研究了各种表面活性剂对碱性阳离子染
料间能量转移的影响，以及转移效率的影响因素。
第三章介绍了荧光能量转移在测定无机离子中的具体应用。第一节吖啶橙—
罗丹明6G能量转移荧光猝灭法测定痕量磷；第二节PAR—吖啶黄能量转移荧光
猝灭法测定痕量铁（Ⅱ）的研究。
第四章罗丹明B-7-碘-8-羟基喹啉-5-磺胶束体系荧光熄灭法测定钒。
第五章苯基荧光酮荧光分光光度法测定粉煤灰中的痕量锗。
This paper consists of five chapters. In the first paper, Application of fluorescence
analysis based on resonance energy transfer (FRET)is reviewed with 56 references. The
review includes Fster theory of fluorescence resonance energy transfer, analysis
techniques and application.
In the second chapter the fluorescence energy transfer between some basic dyes,
such as acridine orange (AO), acridine yellow (AY), rhodamine 6G(Rh6G), rhodamine
B (RhB), ethyl ester of rhodamine B (ERhB), buthyrhodamine B (BRhB) and safranine
T (ST), was investigated. The effect of various surfactants on fluorescence energy
transfer of basic dyes was studied, too. The conditions effecting energy transfer
efficiency were discussed.
Practical application of fluorescence energy transfer to the determination of
inorganic ions was introduced in the third chapter. In the first section, the determination
of trace phosphorus by using energy transfer fluorescence quenching from acridine
orange to rhodamine 60 was studied. In the second section, study on the energy transfer
florescence qtienching among combination of acridine yellow-PAR determination of
trace iron.
In the fourth chapter, determination of trace Vanadium(v) by a fluorescence
quenching method with rhodamine B-7-iodo-8-hydroxyquinoline-5-sulfonic acid
surfactant system was studied.
In the fifth chapter, spectrofluorometric determination of trace germanium with
phenylfluorone in flyash was investigated.
技术及其应用进行了较全面的综述。引用文献56篇。
第二章对碱性荧光染料吖啶橙（AO）、吖啶黄（AY）、罗丹明6G（R6G）、罗丹明
B（RB）、乙基罗丹明B（ERB）、丁基罗丹明B（BRB）、碱性藏花红（ST）组成的各
种染料混合体系的能量转移进行了研究。研究了各种表面活性剂对碱性阳离子染
料间能量转移的影响，以及转移效率的影响因素。
第三章介绍了荧光能量转移在测定无机离子中的具体应用。第一节吖啶橙—
罗丹明6G能量转移荧光猝灭法测定痕量磷；第二节PAR—吖啶黄能量转移荧光
猝灭法测定痕量铁（Ⅱ）的研究。
第四章罗丹明B-7-碘-8-羟基喹啉-5-磺胶束体系荧光熄灭法测定钒。
第五章苯基荧光酮荧光分光光度法测定粉煤灰中的痕量锗。
This paper consists of five chapters. In the first paper, Application of fluorescence
analysis based on resonance energy transfer (FRET)is reviewed with 56 references. The
review includes Fster theory of fluorescence resonance energy transfer, analysis
techniques and application.
In the second chapter the fluorescence energy transfer between some basic dyes,
such as acridine orange (AO), acridine yellow (AY), rhodamine 6G(Rh6G), rhodamine
B (RhB), ethyl ester of rhodamine B (ERhB), buthyrhodamine B (BRhB) and safranine
T (ST), was investigated. The effect of various surfactants on fluorescence energy
transfer of basic dyes was studied, too. The conditions effecting energy transfer
efficiency were discussed.
Practical application of fluorescence energy transfer to the determination of
inorganic ions was introduced in the third chapter. In the first section, the determination
of trace phosphorus by using energy transfer fluorescence quenching from acridine
orange to rhodamine 60 was studied. In the second section, study on the energy transfer
florescence qtienching among combination of acridine yellow-PAR determination of
trace iron.
In the fourth chapter, determination of trace Vanadium(v) by a fluorescence
quenching method with rhodamine B-7-iodo-8-hydroxyquinoline-5-sulfonic acid
surfactant system was studied.
In the fifth chapter, spectrofluorometric determination of trace germanium with
phenylfluorone in flyash was investigated.
引文
1 F(?)ster T. Ann. Phys., 1948,2:55-75
2 Stryer L, Haugland r. Proc. Natl.Acad. Sci.USA, 1967,58:719～726
3．祝大昌，陈剑 宏，朱世盛译．分子发光分析法(荧光法和磷光法)．上海，复旦大学出版社．1985：132
4 Schulman S G. Molecular Luminescence Spectroscopy Method and Applications. Part Ⅰ, John Wiley & Sons, New York, 1985,734
5 Lakowicz J R. Principles of fluorescence spectroscopy, plenum press. New York, 1983,315
6 Kobayashi S, Tamiya E et al. Proc. Electrochem Soc., 1987:87-89,370-372
7 章竹君．能量转移发光分析．分析试验室，1993，12(1)：25
8 魏亦男，李元宗，常文保，慈运祥．荧光共振能量转移技术在生物分析中的应用．分析化学．1999，26(4)：477～484
9 张帆，刘振鸣．胶束在化学发光分析中的应用和发展．分析化学．1989，17(11)：1051～1056
10 David M. G. Energy transfer between dyes-A study using fluorescence quenching and F(?)rster theory. J Chem. Education. 1985, 62(8): 711～714
11 吴世康，姜永才．染料与表面活性物质间的相互作用-在胶束形成前的能量转移．化学学报．1982，40(10)：939
12 何锡文，唐志新，于贵英，杨万龙，张贵珠，史慧明．吖啶橙—罗丹明6G—罗丹明B组合成各种染料体系能量转移的研究，分析化学．1994，22(4)：332～335
13 Singhal G S, Rabinowitch E, Hevesi J, Srinivasan V. Photochem. Photobiol. 1970, 11,531
14 Escabi-Perez J. R., Nome F, Fendler J H. J.Amer. Chem. Soc.1977,99:7749
15 Guptasarma P, Ruman B. Anal. Biochem. 1995,230(1): 187～191
16 Sluis-cremer N, Naidoo N N, Kaplan M H, Manoharan T H. Eur. J, Biochem. 1996, 241(2):484～488
17 Beardsley K, Cantor C R. Proc. Natl. Acad. Sci. USA, 1970,65(1): 39～46
18 Weber G, Teale F W. J. Discuss Faraday Soc. 1959,27:134-141
19 Leonard J J, Yonetani T,Callis J B,Biochem. 1974, 13(6):1460～1464
20 Vanderkooi J M, Adar F, Erecinska M. Eur. J. Biochem. 1976,64(2):381-387
21 Divita G, Goddy R S, Gantheron D C, Pietro A D. J B. Bio.chem. 1993, 268(18)13178-13186
22 Wolf D F, Winiski A P, Bocian B K,Pagano R E. Biochem. ,1992,31 (11):2865-2873
23 Udenfriend S, Stein S, Bohlen P, Dairman W, leimgruber W.Science. 1972,178(4063):871-872
24 陈国珍 黄贤智 郑朱梓 许金钩 王尊本编著 荧光分析法(第二版) 北京，科学出版社，1990：122～132
25 Wangbai Ma, Zhujun Zhang et. al. Chemical Sensors and Microinstrumentation.ACS ,Washington DC, 1989,273
26 鲍所言，王桂华，刘保生等。PAR—吖啶黄能量转移荧光猝灭法测定痕量铁(Ⅱ)的研究．光谱学与光谱分析 2001，21(1)，87-89
27 刘保生，王桂华，孙汉文等。吖啶橙—罗丹明 6G 能量转移荧光猝灭法测定痕量磷．分析化学 2001，28(1)，42-44
28 Latt S A et al. Anal. Bio. Chem. 1972,50:56
29 Chen R F et al. Anal. Lett., 1977,10:787
30 Zhujun Zhang, Seite W R. Anal. Chim. Acta. 1990,236:251
31 Ullman E F, Schwarzberg M et al. J Biol Chem. 1976,251(14):4172-4178
32 Hassan M. Landon, J Immunoassay. 1982,3 (1):1
33 Miller J N, Lim C S et al. Analyst. 980,105:91
34 Karnes H T et al. Clin Chem. 1983, 29:889
35 Shaw E F, Waoton R A A et al. J Clin Pathol. 1977,30:526
36 Snuth D S. FEBS Lett. 1977,77:25
37 Kobayashi y, Tsubota N et al. Steroids. 1980,36:117
38 Vandewerf P, Chang C. J Immund, Mech. 1980,36:339
39 Walt D R, Munkholm C et al. Ibid. ACS press, Washington DC,1989,265
40 Ahuja R C, Budach W et al. Proc Europtrode 1,Graz Austria, 1992,70
41 Issaac V E, Patel L, Curran T, Abate-Shen C. Biochem. 1995,34(46):15276～15281
42 Wistroin C A, Jones G M, Tobias P S, Sklar L A. Biophys, J. 1996,70(2):988～997
43 Dutta A K, Lavoice H, Ohta K, Salrsse C. Langmuir 1997,13 (4): 801～807
44 Parkhurst K M,Parkhurst L J. J Biochem. Opt. 1996,1(4): 435～441
45Steer B A, Merrill A R. Biochem. 1994,33(5) : 1108-1115
46Schwinefus J J, Wang S C, Hamond R W, Morris M J Electrophoresis 1996,17(6) : 1110-1114
47Moens P D J, Yee D J, Remedios C G. Biochem 1994,33:13102-13108
48Chen Q, Lentz B R. Biochem. 1997, 36(15) :4701~4711
49Ozaki H, Iwase N, Sawai H, Kadoma T, Kyugoku Y. Biochem. Biophys. Res. Commun. 1997,231(3) :553~556
50Cai K, Schirch V. J. Bio. Chem., 1996,35(49) :15915-15922
51Keaaler C(Ed.). Nonradioactive Labeling and Detection of Biomolecules. Springer Laboratory, 1992:414
52Sixou S, Szoka F C Jr., Green G A, Aiusti B, Zon G,Chin D J. Nucleic Acids Res., 1994,22(4) :662~668
53Morrison L E. Anal.Biochem. 1989,183:231-244
54Kalyanasundaram K.Chem. Soc. Rev. 1978 , (7) : 453
55 Graevtl MJhomas J K.J.Chem. Soc. 1973 , 95 : 6885
56 Kusumoto Y,Sato H. Chem. Phys. Lett. 1979 , 68 : 13
2 Stryer L, Haugland r. Proc. Natl.Acad. Sci.USA, 1967,58:719～726
3．祝大昌，陈剑 宏，朱世盛译．分子发光分析法(荧光法和磷光法)．上海，复旦大学出版社．1985：132
4 Schulman S G. Molecular Luminescence Spectroscopy Method and Applications. Part Ⅰ, John Wiley & Sons, New York, 1985,734
5 Lakowicz J R. Principles of fluorescence spectroscopy, plenum press. New York, 1983,315
6 Kobayashi S, Tamiya E et al. Proc. Electrochem Soc., 1987:87-89,370-372
7 章竹君．能量转移发光分析．分析试验室，1993，12(1)：25
8 魏亦男，李元宗，常文保，慈运祥．荧光共振能量转移技术在生物分析中的应用．分析化学．1999，26(4)：477～484
9 张帆，刘振鸣．胶束在化学发光分析中的应用和发展．分析化学．1989，17(11)：1051～1056
10 David M. G. Energy transfer between dyes-A study using fluorescence quenching and F(?)rster theory. J Chem. Education. 1985, 62(8): 711～714
11 吴世康，姜永才．染料与表面活性物质间的相互作用-在胶束形成前的能量转移．化学学报．1982，40(10)：939
12 何锡文，唐志新，于贵英，杨万龙，张贵珠，史慧明．吖啶橙—罗丹明6G—罗丹明B组合成各种染料体系能量转移的研究，分析化学．1994，22(4)：332～335
13 Singhal G S, Rabinowitch E, Hevesi J, Srinivasan V. Photochem. Photobiol. 1970, 11,531
14 Escabi-Perez J. R., Nome F, Fendler J H. J.Amer. Chem. Soc.1977,99:7749
15 Guptasarma P, Ruman B. Anal. Biochem. 1995,230(1): 187～191
16 Sluis-cremer N, Naidoo N N, Kaplan M H, Manoharan T H. Eur. J, Biochem. 1996, 241(2):484～488
17 Beardsley K, Cantor C R. Proc. Natl. Acad. Sci. USA, 1970,65(1): 39～46
18 Weber G, Teale F W. J. Discuss Faraday Soc. 1959,27:134-141
19 Leonard J J, Yonetani T,Callis J B,Biochem. 1974, 13(6):1460～1464
20 Vanderkooi J M, Adar F, Erecinska M. Eur. J. Biochem. 1976,64(2):381-387
21 Divita G, Goddy R S, Gantheron D C, Pietro A D. J B. Bio.chem. 1993, 268(18)13178-13186
22 Wolf D F, Winiski A P, Bocian B K,Pagano R E. Biochem. ,1992,31 (11):2865-2873
23 Udenfriend S, Stein S, Bohlen P, Dairman W, leimgruber W.Science. 1972,178(4063):871-872
24 陈国珍 黄贤智 郑朱梓 许金钩 王尊本编著 荧光分析法(第二版) 北京，科学出版社，1990：122～132
25 Wangbai Ma, Zhujun Zhang et. al. Chemical Sensors and Microinstrumentation.ACS ,Washington DC, 1989,273
26 鲍所言，王桂华，刘保生等。PAR—吖啶黄能量转移荧光猝灭法测定痕量铁(Ⅱ)的研究．光谱学与光谱分析 2001，21(1)，87-89
27 刘保生，王桂华，孙汉文等。吖啶橙—罗丹明 6G 能量转移荧光猝灭法测定痕量磷．分析化学 2001，28(1)，42-44
28 Latt S A et al. Anal. Bio. Chem. 1972,50:56
29 Chen R F et al. Anal. Lett., 1977,10:787
30 Zhujun Zhang, Seite W R. Anal. Chim. Acta. 1990,236:251
31 Ullman E F, Schwarzberg M et al. J Biol Chem. 1976,251(14):4172-4178
32 Hassan M. Landon, J Immunoassay. 1982,3 (1):1
33 Miller J N, Lim C S et al. Analyst. 980,105:91
34 Karnes H T et al. Clin Chem. 1983, 29:889
35 Shaw E F, Waoton R A A et al. J Clin Pathol. 1977,30:526
36 Snuth D S. FEBS Lett. 1977,77:25
37 Kobayashi y, Tsubota N et al. Steroids. 1980,36:117
38 Vandewerf P, Chang C. J Immund, Mech. 1980,36:339
39 Walt D R, Munkholm C et al. Ibid. ACS press, Washington DC,1989,265
40 Ahuja R C, Budach W et al. Proc Europtrode 1,Graz Austria, 1992,70
41 Issaac V E, Patel L, Curran T, Abate-Shen C. Biochem. 1995,34(46):15276～15281
42 Wistroin C A, Jones G M, Tobias P S, Sklar L A. Biophys, J. 1996,70(2):988～997
43 Dutta A K, Lavoice H, Ohta K, Salrsse C. Langmuir 1997,13 (4): 801～807
44 Parkhurst K M,Parkhurst L J. J Biochem. Opt. 1996,1(4): 435～441
45Steer B A, Merrill A R. Biochem. 1994,33(5) : 1108-1115
46Schwinefus J J, Wang S C, Hamond R W, Morris M J Electrophoresis 1996,17(6) : 1110-1114
47Moens P D J, Yee D J, Remedios C G. Biochem 1994,33:13102-13108
48Chen Q, Lentz B R. Biochem. 1997, 36(15) :4701~4711
49Ozaki H, Iwase N, Sawai H, Kadoma T, Kyugoku Y. Biochem. Biophys. Res. Commun. 1997,231(3) :553~556
50Cai K, Schirch V. J. Bio. Chem., 1996,35(49) :15915-15922
51Keaaler C(Ed.). Nonradioactive Labeling and Detection of Biomolecules. Springer Laboratory, 1992:414
52Sixou S, Szoka F C Jr., Green G A, Aiusti B, Zon G,Chin D J. Nucleic Acids Res., 1994,22(4) :662~668
53Morrison L E. Anal.Biochem. 1989,183:231-244
54Kalyanasundaram K.Chem. Soc. Rev. 1978 , (7) : 453
55 Graevtl MJhomas J K.J.Chem. Soc. 1973 , 95 : 6885
56 Kusumoto Y,Sato H. Chem. Phys. Lett. 1979 , 68 : 13