硫酸锌对大豆生长发育及产量、品质的影响
摘要
试验于2007年在黑龙江八一农垦大学大豆试验田进行。土壤类型为草甸黑钙土,采用盆栽试验,探讨6个剂量的ZnSO4.7H2O拌种(A1为0g/kg,A2为1g/kg,A3为2g/kg,A4为4g/kg,A5为8g/kg,A6为16g/kg),对大豆的形态建成、光合生理、氮代谢、根系吸收,抗逆生理以及对大豆产量和品质的影响。通过本研究,为合理进行大豆锌营养调控提供科学的依据。
研究结果:
不同剂量的硫酸锌能够不同程度地增加大豆的株高,茎粗,处理A3、A4效果较好;在硫酸锌的作用下,大豆的叶片、叶柄、茎和荚的干物质积累有所增加,处理A3、A4和A5对于增加大豆地上部干物质积累效果较好;根长、根体积、根干重在硫酸锌的作用下都不同程度地增加,处理A2、A4及A5作用较好;处理A4、A5大豆结瘤较多。
在各个生育时期,各个处理对大豆光合生理影响不同,但大多数表现为促进,以中等剂量为效果较好。大豆叶面积、叶绿素a、叶绿素b、叶绿素(a+b)、类胡萝卜素含量及光合参数的值以A2～A5增加显著。剂量A3～A5拌种处理最有利于淀粉和可溶性糖前期的积累和后期的转运。
大多数时期根系活力随剂量发生有规律变化,先随着剂量增加到一定程度后开始逐渐减小,A3～A5对提高大豆伤流量和根系活力的效果较好。
硫酸锌可以增加大豆硝态氮含量,各个生育时期不同处理的影响不同,A3、A4及A5对于增加大豆硝态氮含量效果较好。在硫酸锌作用下,各处理的氨态氮和可溶性蛋白含量均提高了,A3和A4提高较多。
硫酸锌可以在一定范围内增加大豆苗期至结荚期,超氧化物歧化酶,过氧化物酶及过氧化氢酶的活性,各酶的活性先随着硫酸锌剂量增加,当增加到一定程度以后开始随着剂量增加而减少;在鼓粒至成熟期,大多数处理的酶活性都低于对照。大多数处理的丙二醛、脯氨酸含量在硫酸锌作用下降低了,并且,随着生育时期的推进,丙二醛、脯氨酸含量增加缓慢。
适量的硫酸锌可以显著提高大豆的产量性状、产量和品质。处理A3和A4的产量显著高于对照,大多数处理的蛋白质含量得到了显著提高,但脂肪含量增加不显著。
总的来说,适量施锌对大豆地上部的生长,根系的吸收,光合作用的增强及氮代谢都有促进作用,还可以激活大豆保护性酶的活性,降低丙二醛和脯氨酸含量,增强清除体内氧自由基的能力,有效延缓植株衰老,所以,大豆的产量和品质都得到了显著提高。
In 2007,the study was carried in Heilongjiang August First Land Reclamation University Soybean experiment station. The soil type is meadow.The mode of experiment is pots.Through investigating the effect of different dose of ZnSO4.7H2O (A1:0g/kg, A2:1g/kg, A3:2g/kg, A4:4g/kg, A5:8g/kg, A6:16g/kg) on morphology formation,photosynthetic physiology, absorb of root, mechanism of nitrogen assimilation, hardiness mechanism,yield and quality of soybean,we have probed into the scientific evidence for the Znic nutrition adjusting of soybean.
The results as follows:
The different dose of Znic mixed with seeds can improved plant height,the stem diameter,the effect of A3、A4 is best.Accumulation of dry matter of all trentment was improved,A3、A4 and A5 is better,The length of major root and the volume of all trentment were improved,A2、A4 and A5 is better. Nodule of all trentment was improved,A4、A5 is better.
Znic mixed with seeds improved the area of soybean leaf, the content of Chlorophyll a, b, a+b、Carotenoids and photosynthetic parameters of soybean,effect of A2、A5 is most obvious. That of A1～A6 has no significant difference in R5,but averge number of A5 is beetter.Proper dose of Znic improved the content of starch and soluble sugar, provide plenty of nutrition for the seeds.
Znic mixed with seeds improved the bleeding intensity of the soybean plant and root vigor in every growth stage,the effect of A3 and A5 is better. That of most treatments varied with dose in proper limit,while dose of Znic increased,the bleeding intensity and root vigor of soybean plant increased.
Content of NO3-—N, NH4+—N and soluble protein was improved with Znic,NO3-—N of A3、A4 and A5 is higher,NH4+—N and soluble protein of A3 and A4 is higher.
In proper limit,SOD, POD and CAT activity were improved with Znic in V4,R1 and R3.In R5 and R7, SOD, POD and CAT activity of most treatments were decreased.Content of MDA were decreased by Znic. Pro content of most treatments were decreased by Znic.Among these treatments, effect of the moderate dose of Znic is better.
The proper dose of Znic improved the yield, the yield component and quality of soybean.Yield 0f A3 and A4 arrived 5% significance level with CK . Protein of most treatments arrived 5% significance level with CK , the oil didn’t arrived 5% significance level with CK.
In general, proper dose of Znic can improve grond growth of soybean、absorb of soybean root、photosynthetic physiology、mechanism of nitrogen assimilation and protection ferment activity of soybean,decrease content of the MDA、Pro and O- in soybean, so the yield and quality of soybean.
研究结果:
不同剂量的硫酸锌能够不同程度地增加大豆的株高,茎粗,处理A3、A4效果较好;在硫酸锌的作用下,大豆的叶片、叶柄、茎和荚的干物质积累有所增加,处理A3、A4和A5对于增加大豆地上部干物质积累效果较好;根长、根体积、根干重在硫酸锌的作用下都不同程度地增加,处理A2、A4及A5作用较好;处理A4、A5大豆结瘤较多。
在各个生育时期,各个处理对大豆光合生理影响不同,但大多数表现为促进,以中等剂量为效果较好。大豆叶面积、叶绿素a、叶绿素b、叶绿素(a+b)、类胡萝卜素含量及光合参数的值以A2～A5增加显著。剂量A3～A5拌种处理最有利于淀粉和可溶性糖前期的积累和后期的转运。
大多数时期根系活力随剂量发生有规律变化,先随着剂量增加到一定程度后开始逐渐减小,A3～A5对提高大豆伤流量和根系活力的效果较好。
硫酸锌可以增加大豆硝态氮含量,各个生育时期不同处理的影响不同,A3、A4及A5对于增加大豆硝态氮含量效果较好。在硫酸锌作用下,各处理的氨态氮和可溶性蛋白含量均提高了,A3和A4提高较多。
硫酸锌可以在一定范围内增加大豆苗期至结荚期,超氧化物歧化酶,过氧化物酶及过氧化氢酶的活性,各酶的活性先随着硫酸锌剂量增加,当增加到一定程度以后开始随着剂量增加而减少;在鼓粒至成熟期,大多数处理的酶活性都低于对照。大多数处理的丙二醛、脯氨酸含量在硫酸锌作用下降低了,并且,随着生育时期的推进,丙二醛、脯氨酸含量增加缓慢。
适量的硫酸锌可以显著提高大豆的产量性状、产量和品质。处理A3和A4的产量显著高于对照,大多数处理的蛋白质含量得到了显著提高,但脂肪含量增加不显著。
总的来说,适量施锌对大豆地上部的生长,根系的吸收,光合作用的增强及氮代谢都有促进作用,还可以激活大豆保护性酶的活性,降低丙二醛和脯氨酸含量,增强清除体内氧自由基的能力,有效延缓植株衰老,所以,大豆的产量和品质都得到了显著提高。
In 2007,the study was carried in Heilongjiang August First Land Reclamation University Soybean experiment station. The soil type is meadow.The mode of experiment is pots.Through investigating the effect of different dose of ZnSO4.7H2O (A1:0g/kg, A2:1g/kg, A3:2g/kg, A4:4g/kg, A5:8g/kg, A6:16g/kg) on morphology formation,photosynthetic physiology, absorb of root, mechanism of nitrogen assimilation, hardiness mechanism,yield and quality of soybean,we have probed into the scientific evidence for the Znic nutrition adjusting of soybean.
The results as follows:
The different dose of Znic mixed with seeds can improved plant height,the stem diameter,the effect of A3、A4 is best.Accumulation of dry matter of all trentment was improved,A3、A4 and A5 is better,The length of major root and the volume of all trentment were improved,A2、A4 and A5 is better. Nodule of all trentment was improved,A4、A5 is better.
Znic mixed with seeds improved the area of soybean leaf, the content of Chlorophyll a, b, a+b、Carotenoids and photosynthetic parameters of soybean,effect of A2、A5 is most obvious. That of A1～A6 has no significant difference in R5,but averge number of A5 is beetter.Proper dose of Znic improved the content of starch and soluble sugar, provide plenty of nutrition for the seeds.
Znic mixed with seeds improved the bleeding intensity of the soybean plant and root vigor in every growth stage,the effect of A3 and A5 is better. That of most treatments varied with dose in proper limit,while dose of Znic increased,the bleeding intensity and root vigor of soybean plant increased.
Content of NO3-—N, NH4+—N and soluble protein was improved with Znic,NO3-—N of A3、A4 and A5 is higher,NH4+—N and soluble protein of A3 and A4 is higher.
In proper limit,SOD, POD and CAT activity were improved with Znic in V4,R1 and R3.In R5 and R7, SOD, POD and CAT activity of most treatments were decreased.Content of MDA were decreased by Znic. Pro content of most treatments were decreased by Znic.Among these treatments, effect of the moderate dose of Znic is better.
The proper dose of Znic improved the yield, the yield component and quality of soybean.Yield 0f A3 and A4 arrived 5% significance level with CK . Protein of most treatments arrived 5% significance level with CK , the oil didn’t arrived 5% significance level with CK.
In general, proper dose of Znic can improve grond growth of soybean、absorb of soybean root、photosynthetic physiology、mechanism of nitrogen assimilation and protection ferment activity of soybean,decrease content of the MDA、Pro and O- in soybean, so the yield and quality of soybean.
引文
[1] 李兆林,王建国,许艳丽等.微量元素肥料组合对不同基因型大豆产量和品质影响.农业系统科学与综合研究,2004,20(4):281～284
[2] Alina K P,honry K P Trace elements in soil and plants,CRC Press.London,1996.167～291
[3] Cakmak I,Dirci R,Torun B,et al.Role of rye Chromosomes in improvement of zine efficiency in wheat and triticate.Plant and Soil,1997,196:249～253
[4] Khan H R,McDonald G K,Rrngel Z.chickpea Genotypes differ in their sensitunity to Zn deficiency.plant and Soil,1998,198:11～18
[5] 李小梁.微量元素与植物生长.广东微量元素科学,2006 ,13(2):16～16
[6] Harmens H.Physiology of xinc tolerance in Silene vulgar is Febodruk,Enschede,A,sterdam 1993,61～76
[7] 刘峥.微量元素肥料.北京:化学工业出版社 1998
[8] 董钻.大豆产量生理.北京:中国农业出版社 2000
[9] 张秋英.盐碱地大豆施用锌肥的增产效应.现代化农业,1990,7:21～21
[10] 刘铮.微量元素的农业化学.北京:农业出版社 1991
[11] Swaine,D.J.(1955): The Trace Element Content of Soils.Comm.Bur.soil Sci.Tech.Commun.No.48
[12] Vinogradov A.P.(1959):The Geochemistry of Rare and Dispersed Chemical Elements in Soils.Consultants Bureau Inc.,New York
[13] Aubert,H.and M.Pinta(1977):Trace Elements in Soils.Elsevier,New York
[14] Berrow,M.C.and G.A.Reaves ( 1984 ): Background Levels of trace elementsin soils.In“Environmental Contamination”.CEP consultant Ltd.333～340.edinburg,U.K
[15] 刘铮、唐丽华、朱其清等.1978.我国主要土壤中微量元素的含量与分布初步总结,土壤学报,15(2):138～150
[16] 刘铮、朱其清、唐丽华.土壤微量元素.中国土壤(熊毅、李庆逵主编),517～536,北京:科学出版社 1987
[17] 林大仪,黄昌勇.土壤学.北京:中国林业出版社 2002
[18] Zhu Qi Qing,liu Zheng(1981):The status of microelements in paddy soils of China and their role in crop production .III.Zinc.In“Proceedings of Symposium on Paddy Soils”(.Ed.li Qing-kui and Yu Tian-ren.)p635-640:Science Press,Beijing and Springer Verlag,Berlin-Heidelberg-New York.
[19] 刘峥、朱其清、唐丽华、徐俊祥、尹楚良(1982):我国缺乏微量元素的土壤及其区域分布 土壤学报,19(3):209～223
[20] 鲁如坤等.土壤-植物营养学原理和施肥.北京:化学工业出版社 1998
[21] Lyengar,S.S.,D.C.Martens,and W.P.Miller(1981):Distribution and Plant Availability of Soil Znic fractions.S.S.S.A.J.45(4):735～739
[22] Viets,F.G.Jr.(1962):Chemistry and availability of micronutrients in soils.J.Agr.FoodChem.10(3):174～178
[23] Lindsay,W.L.(1979):Chemical Equilibria in soils.Wiley,New York.
[24] Kabata-Pendias,A. ( 1984 ) :Trace Elements in soils and Plants.CRC press,Inc.Boca Raton,Florida.
[25] 蒋廷惠,胡蔼堂,秦怀英.1989.土壤中锌、铜、铁、锰的形态与有效性的关系.土壤通报 ,20(2):86～89
[26] Lindsay,W.L.(1972):Zinc in soils and plant nutrition.Adv.Agron.24:147～181
[27] Follett,R.H.and W.L.Lindsay(1971):Profile distribution of zinc,iron,manganese and copper in Colorado Agric.Exp.Sta.Tech.Bull
[28] Udo,E.J.et al.(1970):Zinc adsorption by calcareous soils.S.S.S.A.Proc.34:405～407
[29] 袁可能编著(1983):植物营养元素的土壤化学.北京:科学出版社
[30] Viets,F.G.,Jr.,L.C.Boawn and C.C.Crawford(1957):The effect of nitrogen and types of nitrogen carrier on plant uptake of indigenous and applied zinc.S.S.S.A.Proc.21:197～201
[31] Mikkelsen,D.S.and S.Kuo(1977):Zinc fertilization and behaviour in flooded soils.Special Publication No.5,Commonwealth Bureau of Soils.
[32] 田春莲,钟晓红.园艺植物锌素营养的研究.安徽农业科学,2005,33(2):331～332
[33] katyal,J.C.and N.S.Randhawa(1983):Micronutrients.FAO Fertilizer and Plant Nutrition Bulletin No.7
[34] 陆欣.土壤肥料学.北京:中国农业大学出版社 2002
[35] 刘春生.土壤肥料学.北京:中国农业大学出版社 2006
[36] 刘福来.土壤—植物系统中锌的研究概况.土壤肥料 1998,(5):10～14
[37] 刘武定.微量元素营养与微肥施用.北京:中国农业出版社 1999
[38] Guliev N M,Bairamov S M,Aliev D A.Functional organization of carbonic anhydrase in higher plants[J].PlantPhysiol,1992,39:537～544.
[39] Moroney J V,Bartlett S G,Samuelsson G.Carbonic anhydrases in plant and algae[J].Plant Cell and Environment,2001,24:141～153.
[40] 季本华,李霞,焦德茂.光抑制条件下水稻叶内碳酸酐酶的适应调节及其生理作用[J].中国水稻科学,1997,11(4):238～240.
[41] Jacobson B S,Fong F,Heath R L.Carbonic anhydrase of spinach[J].Plant Physiol,1975,55:468～474.
[42] Pandey N,Sharma C P.Zinc deficiency effect on photo synthesis and transpiration in safflower and its reversal on Making up the deficiency[J].Indian J Exp Biol,1989,27:376～377.
[43] Shiraiwa Y,Kiuyama M.Role of carbonic anhydrase and identification of the active species of inorganic carbon uti-lized for photosynthesis in Chara corallina[J].Plant Cell Physiol,1989,30(4):581～587.
[44] 王富芳,李路,刘尚义.作物必须微量元素及其生理功能.[J] 作物杂志,1994,(4):34～36.
[45] Ohki K.Zinc concentration in soybean asrelated to growth,photosynthesis,and carbonicanhydrase activity[J].CropSci,1978,18(1-2):79～82.
[46] Sasaki H,Hirse T,W atanabe Y,et al.Carbonic anhydrase activity and CO2-transfer resistance in Zn-dificient rice leaves[J].Plant Physiol,1998,118:929～934.
[47] 董文轩,沈隽,孟繁静.锌铜处理对苹果属植物叶内 CA 活性的影响[J].果树科学,1995,12(1):10～14.
[48] 韩金玲,李雁鸣,马春英.锌对小麦碳酸酐酶活性的影响[J].华北农学报,2003,18(2):21～25.
[49] Rengel Z.Carbonic anhydrase activity in leaves of wheat genotypes differing in Zn efficiency[J].J Plant Physiol,1995,147:251～256.
[50] Dell B,Wilson S A.Zinc nutrition and leaf carbonic anhydrase activity of Eucalyptus maculate seedings and Tri-folium subterraneun[J].Plant and Soil,1989,113:287～290.
[51] 张贵常.锌对番茄叶绿体显微结构的影响与光的关系[J].实验生物学报,1984,17(2):491～495.
[52] 曲桂敏,黄天栋.锌与苹果叶片的显微结构及其超微分布[J].园艺学报,1993,20(4):399～400.
[53] 褚天铎.玉米缺锌的形态解剖表现[J].北京农业科学,1986,(4):11.
[54] 褚天铎.锌素营养对作物叶片解剖结构的影响[J].植物营养与肥料学报,1995,1(1):24～29.
[55] 曲桂敏,黄天栋,顾曼如.苹果叶片缺锌对有关生理生化指标的影响[J].山东农业大学学报,1994,25(1):15～20.
[56] 王永勤,赵鸿钧.施锌对青花菜产量品质影响机理的研究[J].山西农业大学学报,1999,17(3):218～221.
[57] 张雨林,曹敏建,孔庆国.不同施锌量对玉米生长发育及产量的影响[J].国外农学—杂粮作物,1994,(1):47～50.
[58] 陈铭,尹崇仁.锰、锌肥对冬小麦营养效应的研究[J].中国农业科学,1989,22(40):58～64.
[59] 李芳贤,王金林,李玉兰等.锌对夏玉米生长发育及产量影响的研究[J].玉米科学,1999,(7):72～77
[60] Vallee,B.L.and W.E.C.wacker(1970):Metalloprotein.In“The proteins.”(2nd ed),(H.Neurath Ed.)Vol.5,Academic Press,New York
[61] [德]H.马斯纳著,曹一平,陆景陵等译.高等植物的矿质营养.北京:北京农业大学出版社,1988
[62] [美]S.L.蒂斯代尔,W.L.纳尔逊,[加]J.D 毕滕著,金继运,刘荣乐等译.土壤肥力与肥料.北京:农业科技出版社 1998
[63] Ozanne,P.G.(1955):The effect of nitrogen on zinc deficiency in subterranean clover.Aust.J.Biol.Sci.8:47～55
[64] 程锦.棉花氮锌营养关系的研究.土壤通报,1989,(4):169～172
[65] Seatz,L.F.(1960):Zinc availability and uptake by plants as affected by the calcium and magnesium saturation and phosphorus content of the soil.Trans.7th,Int.Congr.soil Sci.,Ⅱ .271～280
[66] RenildesL.F.Fontes and FredR.Cox Zinc-binding peptides as a function of Zn and S in Soybeans volume 155～156,number1/October,1993
[67] Kanwar J S,Chopra S C.Practical agricultural chemistry[M].Delhi-S,1967.29～107
[68] Hemantaranjan A,Garg O K.Effect of zinc fertilization on the senescence of wheat varieties[J].Indian J Plant Physiol,1984,28:239～245.
[69] 褚天铎,刘新保,王淑惠.小麦施锌肥效果及使用技术的研究[J].土壤肥料,1987,(4):24～26
[70] 韩金玲,李雁鸣,马春英.锌对作物生长发育及产量的影响.河北科技师范学院学报 ,2004,18(4):72～74
[71] 曹宏鑫,周银生,王世敬等.春小麦营养平衡施肥技术对于植株营养状况和产量的影响.[J]宁夏农学院学报,1995,16(2):18～25.
[72] 陈铭,尹崇仁.锰、锌肥对冬小麦营养效应的研究[J].中国农业科学,1989,22(40):58～64.
[73] 王慧敏 王正银.蔬菜锌素营养与产量和品质的关系.蔬菜,2005.12:38～40
[74] 李 华.施锌对马铃薯产量和品质的影响.山西农业大学学报,1997,17(3):270～27
[75] 张永清 刘世民.施锌对青花菜产量及品质的影响.山西师大学报,1997,(11)3:39～43
[76] 高俊杰 于新英.施锌对油菜生长产量和品质影响.北方园艺,1998.5:16～17
[77] 董 英 吴其飞 黄达明 吴春笃.茎部施锌对金桔生长和果实品质的影响.农业机械学报, 2003,(34)2:61～68
[78] 曹恭,梁鸣早.锌-平衡栽培体系中植物必需的微量元素.土壤肥料,2003,6:加 2～加 3
[79] 吴明才,肖昌珍.锌素对大豆的生理效应.华北农学报,l992,7(4):73～77
[80] 马道让,曾桂芬.大豆磷锌配施效果及增产机理的研究.土壤通报,1989,2:73~79
[81] 劳家圣,慕晓茹.大豆氮锌营养研究.土壤通报,1990,1:30～40
[82] 张玉先.锌锰铜对大豆产量和品质的影响,中国农学通报,2005,21(9):158～169
[83] 贾彩霞.黄淮区域夏大豆锌锰铁肥效研究.大豆通报,2005,2:13～13
[84] 王 艳,孙 杰,吴俊兰.锌、锰、钼微量元素营养对大豆产量品质的影响.山西农业大学学报,1997,17(2):116～119
[85] 胡春华,胡春燕.不同微量元素肥料对大豆的增产效果.安徽农业科学,2004,32(6):1164
[86] 孙文玉,郑贵仁,贾红.大豆锌肥最佳用量及肥效研究.大豆通报,2000,2:11～11
[87] 潘瑞炽,王晓菁,李娘辉.植物生理学(第四版).北京:高等教育出版社,2002
[88] 张立军,梁宗锁主编.植物生理学.北京:科学出版社,2007
[89] 于立河 李金锋 郑桂萍.粮食作物栽培学.哈尔滨:黑龙江科学技术出版社,2001
[90] 王晓光, 曹敏建, 王伟等.钾对大豆根系形态与生理特性的影响.大豆科学,2005,24(2):126～134
[91] 汪文杰.氮肥用量对雪茄外包皮烟生长发育、光合特性及品质的影响.河南农业大学硕士学位论文,2006,5
[92] 许大全,沈允钢.光合作用与作物产量[A].作物高产高效生理学研究进展[C].北京:科学出版社,1996,17～24
[93] 孟繁静,刘道宏,苏业瑜编著.植物生理生化.中国农业出版社,1992
[94] 梁建萍,牛 远,谢敬斯,张建达.不同海拔华北落叶松针叶三种抗氧化酶活性与光合色素含量,2007,18(7):1414～1419
[95] BARTLEY G E,SCOLNIK P A.Plant carotenoids:pigments for photoprotection,visual attraction,and human health[J].Plant Cell,1995,7(7):1027～1038.
[96] TRACEWELL C A,VRETTOS J S,BAUTISTA J A.Carotenoid photooxidation in photosystemⅡ [ J].Archives of Biochemistry and Biophysics,2001,385(1):61～69.
[97] 赵士杰,李树林.VA 菌根促进青椒生长的生理研究华北农学报,1994,9(1):81～86
[98] 刘鹏,杨玉爱. 油菜在低硼胁迫下生理反应的研究进展[J ].中国油料作物学报,1999 ,21(1):74～78.
[99] 余章机等.锌营养对桑叶产量和品质影响的研究 [J] .广西农业大学学报,1994,13(2):129～133
[100] 李合生主编.现代植物生理学.北京:高等教育出版社,2002
[101] 金成忠,许德威.作为根系活力指标的伤流液简易收集法[J ].植物生理通讯,1959(4):51～531
[102] 杨德俊1,杨卫韵2,刘 鹏3,卢晓红4,徐根娣3.锰对大豆若干生理特性的影响.亚热带植物科学,2005,34(2):8～10.
[103] 马新明.冬小麦根系活力与籽粒灌浆关系研究[J].河南农业大学学报,1990,24(6):269～274
[104] 刘建新.锌对玉米幼苗生长和细胞保护酶活性的影响.甘肃科学学报,2004,16(4):42～45
[105] Leonard Beevers.The role of light and nitrate in the induction of nitrate reductase in radish cotyledons and maiseedlings.PlantPhysiology,1965,40:691～695
[106] 王延书 1,王学义 1,2,郁松林 1,孟 凤 1,肖年湘 1,王春飞 1.水杨酸与热锻炼对葡萄幼苗可溶性糖、游离氨基酸和叶绿素荧光参数的影响.西北农业学报,2007,16(4):29～33
[107] Buchanan B B, Gruissem W, Jones R L. Biochemistry and molecular biology of plants [M]. Beijing: Science Press, 2002
[108] 杨梅,林思祖,黄燕华,姬明,曹光球.邻羟基苯甲酸胁迫下杉木叶片游离氨基酸的变化特征.东北林业大学学报,2007,35(2):40～63
[109] 王晓云,程炳嵩.不同锌水平对姜苗氮代谢的影响[J].山东农业大学学报,1993,24(2):207～210.
[110] Naik M S, Asana R D. Effect of zinc deficiency on the synthesis of protein, mineral uptake and ribonuclease activity in the cotton plant[J]. Indian J. Plant Physiol., 1961, 4: 103～108.
[111] Kitagishi K, Obata H. Effects of zinc deficiency on the nitrogen metabolism of meristematic tissues of rice plants with reference to protein synthesis[J]. Soil Sci. Plant Nutri., 1986,32: 397～405.
[112] 郑少泉,蒋际谋,张泽煌,等.套袋对枇杷果实PAL 、POD、PPO 活性和可溶性蛋白质含量的影响[J ].福建农业学报,2001,16(3):45～47.
[113] 丰明乾,孙俊荣,郭蔼光.小麦灌浆期蛋白质运转与籽粒蛋白质积累的关系.陕西农业科学,1999,(2):1～3.
[114] 李小林,王永锐.免耕水稻的光合特性.中山大学学报,1989,(5):152～158
[115] 高必达,陈捷主编.生理植物病理学.北京:科学出版社,2006 157～158
[116] 王夔等.生命科学中的微量元素(下)[M].北京:中国计量出版社,1989:129～130
[117] 杨秀清等.铜锌元素对华北落叶松苗期酶活性影响[J].山西农业大学学报,2001,21(3):277～280
[118] 李俊英等,华北落叶松铜锌元素与酶活性关系初探[J].山西林业科技,2001,(3):35～38
[119] 姚延寿等.华北落叶松铜锌元素与酶活性研究[J].山西农业大学学报,2002,(1):61～64
[120] 高质1,2,林葆1,周卫1.锌素营养对春玉米内源激素与氧自由基代谢的影响.植物营养与肥料学报,2001,7(4):424～428
[121] 潘瑞炽主编.植物生理学.第四版.北京:高等教育出版社,2001
[122] 杨娟,孙莹.中国大豆对外贸易竞争力分析及建议.科技与管理,2007,1:4～6
[123] 李友华.黑龙江省粮食主产区面临的问题及其解决的对策.黑龙江粮食,2003(1):8～12
[124] 张福锁.植物营养生态学和遗传学.北京:中国科学技术出版社,1993 :114～137
[125] 王景安,张福锁.供锌水平对玉米幼苗生长发育及锌含量的影响.吉林农业大学学报 ,2000,22(1):69～72
[126] 杨凤娟,刘世琦,王秀峰,臧金波.锌对大蒜生理生化指标及营养品质的影响.土壤肥料, 2005,1:35～38
[127] 施木田,陈如凯.锌对苦瓜叶片碳代谢及相关酶活性的影响研究.中国生态农业学报, 2004,12(1):56～58
[128] 施木田,陈如凯.锌钼营养对苦瓜产量、品质及叶片氮素代谢的影响.热带作物学报, 2003,24(4):57～61
[129] 王学军.铜、锌元素对华北落叶松体内保护酶活性的影响.山西农业大学硕士研究生毕业论文,2004
[130] 韩金玲.锌对冬小麦光合特性及产量和品质的影响.河北农业大学硕士学位论文,2003
[131] Naik M S,Asana R D.Effect of Zinc deficiency on the synthesis of protein,mineral uptake and ribonuclease activity in the cotton plant Indian J Plant physiol,1961,4:103～108
[132] Kitagishi K, Obata H.Effect of zinc deficiency on the nitrogen metabolism of meristermatic tissues of rice plants with reference to proten synthesis.Soil Sci Plant Nutr,1986,32:397～405
[133] Prask J A.Plocke D J.A role for Znic in the structural intergrity of cytoplasmic ribosomes of Euglena gracilis.Plant Physiol,1971,48:150～155
[134] Falchuk K H,Hardy C,Ulpino L,Vallee B L.RNA metabolism,manganese,and RNA polymerases of zinc sufficient and zinc deficient Euglena gracilis[J].Proc Nat Acda SciUSA,1978,75:4175～4179
[135] Dwivedi R S,Takkar P N.Ribonuclease activity as index of hidden hunger of zinc in crops[J].Plant and Soil,1974,40:170～181.
[136] Cakmak I and Marschner H. Enhanced superoxide radical production in root of Zinc deficient plant. J. Exp. Bot.1988,39:1449～1460
[137] 张玉琼等.锌营养对淹水玉米抗性的影响.安徽农业通报,1999,5(3):19～21
[138] 吴志辉.微肥对作物产量和品质的影响.湖南农业科学,1999,(2):43～43
[2] Alina K P,honry K P Trace elements in soil and plants,CRC Press.London,1996.167～291
[3] Cakmak I,Dirci R,Torun B,et al.Role of rye Chromosomes in improvement of zine efficiency in wheat and triticate.Plant and Soil,1997,196:249～253
[4] Khan H R,McDonald G K,Rrngel Z.chickpea Genotypes differ in their sensitunity to Zn deficiency.plant and Soil,1998,198:11～18
[5] 李小梁.微量元素与植物生长.广东微量元素科学,2006 ,13(2):16～16
[6] Harmens H.Physiology of xinc tolerance in Silene vulgar is Febodruk,Enschede,A,sterdam 1993,61～76
[7] 刘峥.微量元素肥料.北京:化学工业出版社 1998
[8] 董钻.大豆产量生理.北京:中国农业出版社 2000
[9] 张秋英.盐碱地大豆施用锌肥的增产效应.现代化农业,1990,7:21～21
[10] 刘铮.微量元素的农业化学.北京:农业出版社 1991
[11] Swaine,D.J.(1955): The Trace Element Content of Soils.Comm.Bur.soil Sci.Tech.Commun.No.48
[12] Vinogradov A.P.(1959):The Geochemistry of Rare and Dispersed Chemical Elements in Soils.Consultants Bureau Inc.,New York
[13] Aubert,H.and M.Pinta(1977):Trace Elements in Soils.Elsevier,New York
[14] Berrow,M.C.and G.A.Reaves ( 1984 ): Background Levels of trace elementsin soils.In“Environmental Contamination”.CEP consultant Ltd.333～340.edinburg,U.K
[15] 刘铮、唐丽华、朱其清等.1978.我国主要土壤中微量元素的含量与分布初步总结,土壤学报,15(2):138～150
[16] 刘铮、朱其清、唐丽华.土壤微量元素.中国土壤(熊毅、李庆逵主编),517～536,北京:科学出版社 1987
[17] 林大仪,黄昌勇.土壤学.北京:中国林业出版社 2002
[18] Zhu Qi Qing,liu Zheng(1981):The status of microelements in paddy soils of China and their role in crop production .III.Zinc.In“Proceedings of Symposium on Paddy Soils”(.Ed.li Qing-kui and Yu Tian-ren.)p635-640:Science Press,Beijing and Springer Verlag,Berlin-Heidelberg-New York.
[19] 刘峥、朱其清、唐丽华、徐俊祥、尹楚良(1982):我国缺乏微量元素的土壤及其区域分布 土壤学报,19(3):209～223
[20] 鲁如坤等.土壤-植物营养学原理和施肥.北京:化学工业出版社 1998
[21] Lyengar,S.S.,D.C.Martens,and W.P.Miller(1981):Distribution and Plant Availability of Soil Znic fractions.S.S.S.A.J.45(4):735～739
[22] Viets,F.G.Jr.(1962):Chemistry and availability of micronutrients in soils.J.Agr.FoodChem.10(3):174～178
[23] Lindsay,W.L.(1979):Chemical Equilibria in soils.Wiley,New York.
[24] Kabata-Pendias,A. ( 1984 ) :Trace Elements in soils and Plants.CRC press,Inc.Boca Raton,Florida.
[25] 蒋廷惠,胡蔼堂,秦怀英.1989.土壤中锌、铜、铁、锰的形态与有效性的关系.土壤通报 ,20(2):86～89
[26] Lindsay,W.L.(1972):Zinc in soils and plant nutrition.Adv.Agron.24:147～181
[27] Follett,R.H.and W.L.Lindsay(1971):Profile distribution of zinc,iron,manganese and copper in Colorado Agric.Exp.Sta.Tech.Bull
[28] Udo,E.J.et al.(1970):Zinc adsorption by calcareous soils.S.S.S.A.Proc.34:405～407
[29] 袁可能编著(1983):植物营养元素的土壤化学.北京:科学出版社
[30] Viets,F.G.,Jr.,L.C.Boawn and C.C.Crawford(1957):The effect of nitrogen and types of nitrogen carrier on plant uptake of indigenous and applied zinc.S.S.S.A.Proc.21:197～201
[31] Mikkelsen,D.S.and S.Kuo(1977):Zinc fertilization and behaviour in flooded soils.Special Publication No.5,Commonwealth Bureau of Soils.
[32] 田春莲,钟晓红.园艺植物锌素营养的研究.安徽农业科学,2005,33(2):331～332
[33] katyal,J.C.and N.S.Randhawa(1983):Micronutrients.FAO Fertilizer and Plant Nutrition Bulletin No.7
[34] 陆欣.土壤肥料学.北京:中国农业大学出版社 2002
[35] 刘春生.土壤肥料学.北京:中国农业大学出版社 2006
[36] 刘福来.土壤—植物系统中锌的研究概况.土壤肥料 1998,(5):10～14
[37] 刘武定.微量元素营养与微肥施用.北京:中国农业出版社 1999
[38] Guliev N M,Bairamov S M,Aliev D A.Functional organization of carbonic anhydrase in higher plants[J].PlantPhysiol,1992,39:537～544.
[39] Moroney J V,Bartlett S G,Samuelsson G.Carbonic anhydrases in plant and algae[J].Plant Cell and Environment,2001,24:141～153.
[40] 季本华,李霞,焦德茂.光抑制条件下水稻叶内碳酸酐酶的适应调节及其生理作用[J].中国水稻科学,1997,11(4):238～240.
[41] Jacobson B S,Fong F,Heath R L.Carbonic anhydrase of spinach[J].Plant Physiol,1975,55:468～474.
[42] Pandey N,Sharma C P.Zinc deficiency effect on photo synthesis and transpiration in safflower and its reversal on Making up the deficiency[J].Indian J Exp Biol,1989,27:376～377.
[43] Shiraiwa Y,Kiuyama M.Role of carbonic anhydrase and identification of the active species of inorganic carbon uti-lized for photosynthesis in Chara corallina[J].Plant Cell Physiol,1989,30(4):581～587.
[44] 王富芳,李路,刘尚义.作物必须微量元素及其生理功能.[J] 作物杂志,1994,(4):34～36.
[45] Ohki K.Zinc concentration in soybean asrelated to growth,photosynthesis,and carbonicanhydrase activity[J].CropSci,1978,18(1-2):79～82.
[46] Sasaki H,Hirse T,W atanabe Y,et al.Carbonic anhydrase activity and CO2-transfer resistance in Zn-dificient rice leaves[J].Plant Physiol,1998,118:929～934.
[47] 董文轩,沈隽,孟繁静.锌铜处理对苹果属植物叶内 CA 活性的影响[J].果树科学,1995,12(1):10～14.
[48] 韩金玲,李雁鸣,马春英.锌对小麦碳酸酐酶活性的影响[J].华北农学报,2003,18(2):21～25.
[49] Rengel Z.Carbonic anhydrase activity in leaves of wheat genotypes differing in Zn efficiency[J].J Plant Physiol,1995,147:251～256.
[50] Dell B,Wilson S A.Zinc nutrition and leaf carbonic anhydrase activity of Eucalyptus maculate seedings and Tri-folium subterraneun[J].Plant and Soil,1989,113:287～290.
[51] 张贵常.锌对番茄叶绿体显微结构的影响与光的关系[J].实验生物学报,1984,17(2):491～495.
[52] 曲桂敏,黄天栋.锌与苹果叶片的显微结构及其超微分布[J].园艺学报,1993,20(4):399～400.
[53] 褚天铎.玉米缺锌的形态解剖表现[J].北京农业科学,1986,(4):11.
[54] 褚天铎.锌素营养对作物叶片解剖结构的影响[J].植物营养与肥料学报,1995,1(1):24～29.
[55] 曲桂敏,黄天栋,顾曼如.苹果叶片缺锌对有关生理生化指标的影响[J].山东农业大学学报,1994,25(1):15～20.
[56] 王永勤,赵鸿钧.施锌对青花菜产量品质影响机理的研究[J].山西农业大学学报,1999,17(3):218～221.
[57] 张雨林,曹敏建,孔庆国.不同施锌量对玉米生长发育及产量的影响[J].国外农学—杂粮作物,1994,(1):47～50.
[58] 陈铭,尹崇仁.锰、锌肥对冬小麦营养效应的研究[J].中国农业科学,1989,22(40):58～64.
[59] 李芳贤,王金林,李玉兰等.锌对夏玉米生长发育及产量影响的研究[J].玉米科学,1999,(7):72～77
[60] Vallee,B.L.and W.E.C.wacker(1970):Metalloprotein.In“The proteins.”(2nd ed),(H.Neurath Ed.)Vol.5,Academic Press,New York
[61] [德]H.马斯纳著,曹一平,陆景陵等译.高等植物的矿质营养.北京:北京农业大学出版社,1988
[62] [美]S.L.蒂斯代尔,W.L.纳尔逊,[加]J.D 毕滕著,金继运,刘荣乐等译.土壤肥力与肥料.北京:农业科技出版社 1998
[63] Ozanne,P.G.(1955):The effect of nitrogen on zinc deficiency in subterranean clover.Aust.J.Biol.Sci.8:47～55
[64] 程锦.棉花氮锌营养关系的研究.土壤通报,1989,(4):169～172
[65] Seatz,L.F.(1960):Zinc availability and uptake by plants as affected by the calcium and magnesium saturation and phosphorus content of the soil.Trans.7th,Int.Congr.soil Sci.,Ⅱ .271～280
[66] RenildesL.F.Fontes and FredR.Cox Zinc-binding peptides as a function of Zn and S in Soybeans volume 155～156,number1/October,1993
[67] Kanwar J S,Chopra S C.Practical agricultural chemistry[M].Delhi-S,1967.29～107
[68] Hemantaranjan A,Garg O K.Effect of zinc fertilization on the senescence of wheat varieties[J].Indian J Plant Physiol,1984,28:239～245.
[69] 褚天铎,刘新保,王淑惠.小麦施锌肥效果及使用技术的研究[J].土壤肥料,1987,(4):24～26
[70] 韩金玲,李雁鸣,马春英.锌对作物生长发育及产量的影响.河北科技师范学院学报 ,2004,18(4):72～74
[71] 曹宏鑫,周银生,王世敬等.春小麦营养平衡施肥技术对于植株营养状况和产量的影响.[J]宁夏农学院学报,1995,16(2):18～25.
[72] 陈铭,尹崇仁.锰、锌肥对冬小麦营养效应的研究[J].中国农业科学,1989,22(40):58～64.
[73] 王慧敏 王正银.蔬菜锌素营养与产量和品质的关系.蔬菜,2005.12:38～40
[74] 李 华.施锌对马铃薯产量和品质的影响.山西农业大学学报,1997,17(3):270～27
[75] 张永清 刘世民.施锌对青花菜产量及品质的影响.山西师大学报,1997,(11)3:39～43
[76] 高俊杰 于新英.施锌对油菜生长产量和品质影响.北方园艺,1998.5:16～17
[77] 董 英 吴其飞 黄达明 吴春笃.茎部施锌对金桔生长和果实品质的影响.农业机械学报, 2003,(34)2:61～68
[78] 曹恭,梁鸣早.锌-平衡栽培体系中植物必需的微量元素.土壤肥料,2003,6:加 2～加 3
[79] 吴明才,肖昌珍.锌素对大豆的生理效应.华北农学报,l992,7(4):73～77
[80] 马道让,曾桂芬.大豆磷锌配施效果及增产机理的研究.土壤通报,1989,2:73~79
[81] 劳家圣,慕晓茹.大豆氮锌营养研究.土壤通报,1990,1:30～40
[82] 张玉先.锌锰铜对大豆产量和品质的影响,中国农学通报,2005,21(9):158～169
[83] 贾彩霞.黄淮区域夏大豆锌锰铁肥效研究.大豆通报,2005,2:13～13
[84] 王 艳,孙 杰,吴俊兰.锌、锰、钼微量元素营养对大豆产量品质的影响.山西农业大学学报,1997,17(2):116～119
[85] 胡春华,胡春燕.不同微量元素肥料对大豆的增产效果.安徽农业科学,2004,32(6):1164
[86] 孙文玉,郑贵仁,贾红.大豆锌肥最佳用量及肥效研究.大豆通报,2000,2:11～11
[87] 潘瑞炽,王晓菁,李娘辉.植物生理学(第四版).北京:高等教育出版社,2002
[88] 张立军,梁宗锁主编.植物生理学.北京:科学出版社,2007
[89] 于立河 李金锋 郑桂萍.粮食作物栽培学.哈尔滨:黑龙江科学技术出版社,2001
[90] 王晓光, 曹敏建, 王伟等.钾对大豆根系形态与生理特性的影响.大豆科学,2005,24(2):126～134
[91] 汪文杰.氮肥用量对雪茄外包皮烟生长发育、光合特性及品质的影响.河南农业大学硕士学位论文,2006,5
[92] 许大全,沈允钢.光合作用与作物产量[A].作物高产高效生理学研究进展[C].北京:科学出版社,1996,17～24
[93] 孟繁静,刘道宏,苏业瑜编著.植物生理生化.中国农业出版社,1992
[94] 梁建萍,牛 远,谢敬斯,张建达.不同海拔华北落叶松针叶三种抗氧化酶活性与光合色素含量,2007,18(7):1414～1419
[95] BARTLEY G E,SCOLNIK P A.Plant carotenoids:pigments for photoprotection,visual attraction,and human health[J].Plant Cell,1995,7(7):1027～1038.
[96] TRACEWELL C A,VRETTOS J S,BAUTISTA J A.Carotenoid photooxidation in photosystemⅡ [ J].Archives of Biochemistry and Biophysics,2001,385(1):61～69.
[97] 赵士杰,李树林.VA 菌根促进青椒生长的生理研究华北农学报,1994,9(1):81～86
[98] 刘鹏,杨玉爱. 油菜在低硼胁迫下生理反应的研究进展[J ].中国油料作物学报,1999 ,21(1):74～78.
[99] 余章机等.锌营养对桑叶产量和品质影响的研究 [J] .广西农业大学学报,1994,13(2):129～133
[100] 李合生主编.现代植物生理学.北京:高等教育出版社,2002
[101] 金成忠,许德威.作为根系活力指标的伤流液简易收集法[J ].植物生理通讯,1959(4):51～531
[102] 杨德俊1,杨卫韵2,刘 鹏3,卢晓红4,徐根娣3.锰对大豆若干生理特性的影响.亚热带植物科学,2005,34(2):8～10.
[103] 马新明.冬小麦根系活力与籽粒灌浆关系研究[J].河南农业大学学报,1990,24(6):269～274
[104] 刘建新.锌对玉米幼苗生长和细胞保护酶活性的影响.甘肃科学学报,2004,16(4):42～45
[105] Leonard Beevers.The role of light and nitrate in the induction of nitrate reductase in radish cotyledons and maiseedlings.PlantPhysiology,1965,40:691～695
[106] 王延书 1,王学义 1,2,郁松林 1,孟 凤 1,肖年湘 1,王春飞 1.水杨酸与热锻炼对葡萄幼苗可溶性糖、游离氨基酸和叶绿素荧光参数的影响.西北农业学报,2007,16(4):29～33
[107] Buchanan B B, Gruissem W, Jones R L. Biochemistry and molecular biology of plants [M]. Beijing: Science Press, 2002
[108] 杨梅,林思祖,黄燕华,姬明,曹光球.邻羟基苯甲酸胁迫下杉木叶片游离氨基酸的变化特征.东北林业大学学报,2007,35(2):40～63
[109] 王晓云,程炳嵩.不同锌水平对姜苗氮代谢的影响[J].山东农业大学学报,1993,24(2):207～210.
[110] Naik M S, Asana R D. Effect of zinc deficiency on the synthesis of protein, mineral uptake and ribonuclease activity in the cotton plant[J]. Indian J. Plant Physiol., 1961, 4: 103～108.
[111] Kitagishi K, Obata H. Effects of zinc deficiency on the nitrogen metabolism of meristematic tissues of rice plants with reference to protein synthesis[J]. Soil Sci. Plant Nutri., 1986,32: 397～405.
[112] 郑少泉,蒋际谋,张泽煌,等.套袋对枇杷果实PAL 、POD、PPO 活性和可溶性蛋白质含量的影响[J ].福建农业学报,2001,16(3):45～47.
[113] 丰明乾,孙俊荣,郭蔼光.小麦灌浆期蛋白质运转与籽粒蛋白质积累的关系.陕西农业科学,1999,(2):1～3.
[114] 李小林,王永锐.免耕水稻的光合特性.中山大学学报,1989,(5):152～158
[115] 高必达,陈捷主编.生理植物病理学.北京:科学出版社,2006 157～158
[116] 王夔等.生命科学中的微量元素(下)[M].北京:中国计量出版社,1989:129～130
[117] 杨秀清等.铜锌元素对华北落叶松苗期酶活性影响[J].山西农业大学学报,2001,21(3):277～280
[118] 李俊英等,华北落叶松铜锌元素与酶活性关系初探[J].山西林业科技,2001,(3):35～38
[119] 姚延寿等.华北落叶松铜锌元素与酶活性研究[J].山西农业大学学报,2002,(1):61～64
[120] 高质1,2,林葆1,周卫1.锌素营养对春玉米内源激素与氧自由基代谢的影响.植物营养与肥料学报,2001,7(4):424～428
[121] 潘瑞炽主编.植物生理学.第四版.北京:高等教育出版社,2001
[122] 杨娟,孙莹.中国大豆对外贸易竞争力分析及建议.科技与管理,2007,1:4～6
[123] 李友华.黑龙江省粮食主产区面临的问题及其解决的对策.黑龙江粮食,2003(1):8～12
[124] 张福锁.植物营养生态学和遗传学.北京:中国科学技术出版社,1993 :114～137
[125] 王景安,张福锁.供锌水平对玉米幼苗生长发育及锌含量的影响.吉林农业大学学报 ,2000,22(1):69～72
[126] 杨凤娟,刘世琦,王秀峰,臧金波.锌对大蒜生理生化指标及营养品质的影响.土壤肥料, 2005,1:35～38
[127] 施木田,陈如凯.锌对苦瓜叶片碳代谢及相关酶活性的影响研究.中国生态农业学报, 2004,12(1):56～58
[128] 施木田,陈如凯.锌钼营养对苦瓜产量、品质及叶片氮素代谢的影响.热带作物学报, 2003,24(4):57～61
[129] 王学军.铜、锌元素对华北落叶松体内保护酶活性的影响.山西农业大学硕士研究生毕业论文,2004
[130] 韩金玲.锌对冬小麦光合特性及产量和品质的影响.河北农业大学硕士学位论文,2003
[131] Naik M S,Asana R D.Effect of Zinc deficiency on the synthesis of protein,mineral uptake and ribonuclease activity in the cotton plant Indian J Plant physiol,1961,4:103～108
[132] Kitagishi K, Obata H.Effect of zinc deficiency on the nitrogen metabolism of meristermatic tissues of rice plants with reference to proten synthesis.Soil Sci Plant Nutr,1986,32:397～405
[133] Prask J A.Plocke D J.A role for Znic in the structural intergrity of cytoplasmic ribosomes of Euglena gracilis.Plant Physiol,1971,48:150～155
[134] Falchuk K H,Hardy C,Ulpino L,Vallee B L.RNA metabolism,manganese,and RNA polymerases of zinc sufficient and zinc deficient Euglena gracilis[J].Proc Nat Acda SciUSA,1978,75:4175～4179
[135] Dwivedi R S,Takkar P N.Ribonuclease activity as index of hidden hunger of zinc in crops[J].Plant and Soil,1974,40:170～181.
[136] Cakmak I and Marschner H. Enhanced superoxide radical production in root of Zinc deficient plant. J. Exp. Bot.1988,39:1449～1460
[137] 张玉琼等.锌营养对淹水玉米抗性的影响.安徽农业通报,1999,5(3):19～21
[138] 吴志辉.微肥对作物产量和品质的影响.湖南农业科学,1999,(2):43～43