1周热适应和Vc对热应激大鼠骨骼肌及血清能量代谢相关指标影响的研究
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摘要
1研究目的
通过观察热适应和服用Vc对大鼠能量代谢相关指标的影响,探讨热适应、Vc对大鼠能量代谢的影响及其作用机理,为提高运动员在高温环境中运动能力提供理论基础。
2研究方法
8周龄Wistar大鼠120只,体重200±15g,随机分为4组：A(对照组)、B(运动组)、C(运动+热适应组)、D(运动+热适应+Vc组)；B、C、D组大鼠进行一般跑台训练5周、第6周对C、D组进行热适应1周,温度38℃,并给D组灌服Vc,每周递增运动训练时间：第1周训练时间为l0min,速度为10m／min；之后每周时间递增l0min,速度递增3m；第4、5、6周训练时间为40min,速度为21m/min。按安静状态、定量负荷状态、力竭状态,运动后即刻处死,取骨骼肌、血清；测股四头肌、腓肠肌组织中的Na+-K+ATPase、CK、LD、LDH、SDH活性。所有数据用SPSS10.0统计软件分析,显著水平为p<0.05。
3实验结果
3.1安静状态
骨骼肌组织内Na+-K+ATPase活性,运动+热适应组极显著高于对照组(P<0.01)；运动+热适应+Vc组极显著高于其他3组(P<0.01)；血清组织内CK活性,运动+热适应组显著高于对照组(P<0.05)；运动+热适应+Vc组显著高于对照组(P<0.05)；血清组织内LD含量、LDH活性无显著性差异(P>0.05)；SDH活性运动+热适应组显著高于对照组；运动+热适应+Vc组极显著高于其他3组(P<0.01)。
3.2定量运动1小时
骨骼肌组织内Na+-K+ATPase活性,运动+热适应组极显著高于对照组(P<0.01)；运动+热适应+Vc组极显著高于其他3组(P<0.01)；血清组织内CK活性,运动组、运动+热适应组显著低于对照组(p<0.05)。血清组织内LD含量、LDH活性没有显著性差异；(P>0.05)；SDH含量运动+热适应组显著高于对照组(p<0.05)；运+热+VC极显著高于对照组和运动组(P<0.01)。
3.3力竭后即刻
骨骼肌组织内Na+-K+ATPase活性,运动+热适应组显著高于对照组(P<0.05)；运动+热适应+Vc组极显著高于对照组和运动组(P<0.01)；运动+热适应+Vc组显著高于运动+热适应组(P<0.05)。血清组织内CK活性,运动组、运动+热适应、运动+热适应+Vc组极显著低于对照组(p<0.01)。血清组织中LD含量、LDH活性,没有显著性差异；(P>0.05)；SDH活性运动+热适应组显著高于对照组(p<0.05)；运+热+VC极显著高于对照组(P<0.01)。
4结论
4.1热适应1周和Vc可以显著提高热应激大鼠Na+-K+-ATPase活性,特别是热适应和Vc联合作用下可以极显著提高Na+-K+-ATPase、SDH活性,说明热适应1周和Vc联合作用对热应激大鼠有氧代谢能力的提高具有极显著的效果。
4.2安静状态下,热适应1周的热应激大鼠血清CK活性显著升高,在定量负荷和力竭状态下CK活性升高不明显,说明热环境对机体能造成损害,但热适应—周可以降低这种损伤。
4.3 Vc是强还原剂具有清除自由基保护机体的作用,可以显著提高热应激大鼠的代谢能力,但是无法防御高温环境下的机体损伤。热适应1周防御高温环境对热应激大鼠损伤,但提高大鼠代谢能力的效果比Vc差。因此只有热适应和Vc共同作用才能显著提高热应激大鼠的能量代谢及降低损伤。
1 Objective:
By observing the thermal adaptation and energy metabolism in rats taking Vc related indicators on the impact of heat adaptation, Vc energy metabolism in rats and its mechanism in order to enhance sport athlete in high temperature environment, ability to provide a theoretical basis.
2 Methods
8-week-old Wistar rats were 108, weight 200±15g, were randomly divided into 4 groups:A (normal control group), B (exercise group), C (exercise+heat acclimation group), D (exercise+heat adaptation+Vc group); B, C, D rats were generally treadmill training for 5 weeks,6 weeks on the C, D groups heat adaptation (38℃) trained and fed to the D group Vc, increasing exercise training hours per week:1 week of training time as 10min, speed 10m/min; weekly time increments after 10min, the speed increase 3m; the first week of training time for the 4,5,6 40min, speed of 21m/min. By resting state, fixed load condition, state of exhaustion, immediately after exercise were killed, skeletal muscle, serum, measured quadriceps, gastrocnemius muscle tissue Na+-K+ATPase, CK, LD, LDH, SDH activity. SPSS 10.0 statistical software with all the data analysis, the significant level of p<0.05.
3 results
3.1 resting state Within the skeletal muscle Na+-K+ATPase activity, exercise+heat adaptation was significantly higher (P<0.01); movement adaptation+Vc+heat group was significantly higher than the other 3 groups (P<0.01); serum tissue in CK activity, motion and heat adaptation was significantly higher (P<0.05); movement adaptation+Vc+heat group was significantly higher (P<0.05); serum levels within the organization LD, LDH activity was not significantly different (P> 0.05); SDH activity exercise+heat adaptation was significantly higher; movement adaptation+ Vc+heat group was significantly higher than the other 3 groups (P<0.01).
3.2 Quantitative exercise 1 hour Within the skeletal muscle Na+-K+ATPase activity, exercise+heat adaptation was significantly higher (P<0.01); movement adaptation+ Vc+heat group was significantly higher than the other 3 groups (P<0.01); serum tissue CK activity in the exercise group, exercise+heat adaptation was significantly lower than the control group (p<0.05). Serum levels within the organization LD, LDH activity were not significantly different; (P> 0.05); SDH content exercise+heat adaptation was significantly higher (p<0.05); Games+hot+VC was significantly higher and the movement group (P<0.01).
3.3 immediately after exhaustive Within the skeletal muscle Na+-K+ATPase activity, exercise+heat adaptation was significantly higher (P<0.05); movement adaptation+Vc+heat group was significantly higher and the exercise group (P <0.01); Sports+heat adaptation+Vc was significantly higher than the exercise+ heat acclimation group (P<0.05). Serum CK activity within the organization, sports group, motion and heat adaptation, motion and heat adaptation+Vc group was significantly lower than the control group (p<0.01). Tissue content of serum LD, LDH activity, no significant difference; (P> 0.05); SDH activity exercise+heat adaptation was significantly higher (p<0.05); Games+hot+VC was significantly higher (P<0.01).
4 Conclusion
4.1 Thermal adaptation and 1 week and Vc can significantly improve the heat stress in rats Na+-K+-ATPase activity, in particular, thermal adaptation and Vc combined effect may be significantly increased Na+-K+-ATPase, SDH activity, indicating thermal adaptation 1 week and Vc combined effects of heat stress to improve aerobic capacity in rats with highly significant results.
4.2 at rest, heat 1 week to adapt to the heat stress in rats significantly increased serum CK activity in the quantitative load and CK activity increased after exhaustive state is not obvious, indicating the thermal environment of the body can cause damage, but heat adaptation week can reduce this damage.
4.3 Vc is a strong reducing agent has a clear role of free radicals protecting the body, but can not defense the body under high temperature damage.
通过观察热适应和服用Vc对大鼠能量代谢相关指标的影响,探讨热适应、Vc对大鼠能量代谢的影响及其作用机理,为提高运动员在高温环境中运动能力提供理论基础。
2研究方法
8周龄Wistar大鼠120只,体重200±15g,随机分为4组：A(对照组)、B(运动组)、C(运动+热适应组)、D(运动+热适应+Vc组)；B、C、D组大鼠进行一般跑台训练5周、第6周对C、D组进行热适应1周,温度38℃,并给D组灌服Vc,每周递增运动训练时间：第1周训练时间为l0min,速度为10m／min；之后每周时间递增l0min,速度递增3m；第4、5、6周训练时间为40min,速度为21m/min。按安静状态、定量负荷状态、力竭状态,运动后即刻处死,取骨骼肌、血清；测股四头肌、腓肠肌组织中的Na+-K+ATPase、CK、LD、LDH、SDH活性。所有数据用SPSS10.0统计软件分析,显著水平为p<0.05。
3实验结果
3.1安静状态
骨骼肌组织内Na+-K+ATPase活性,运动+热适应组极显著高于对照组(P<0.01)；运动+热适应+Vc组极显著高于其他3组(P<0.01)；血清组织内CK活性,运动+热适应组显著高于对照组(P<0.05)；运动+热适应+Vc组显著高于对照组(P<0.05)；血清组织内LD含量、LDH活性无显著性差异(P>0.05)；SDH活性运动+热适应组显著高于对照组；运动+热适应+Vc组极显著高于其他3组(P<0.01)。
3.2定量运动1小时
骨骼肌组织内Na+-K+ATPase活性,运动+热适应组极显著高于对照组(P<0.01)；运动+热适应+Vc组极显著高于其他3组(P<0.01)；血清组织内CK活性,运动组、运动+热适应组显著低于对照组(p<0.05)。血清组织内LD含量、LDH活性没有显著性差异；(P>0.05)；SDH含量运动+热适应组显著高于对照组(p<0.05)；运+热+VC极显著高于对照组和运动组(P<0.01)。
3.3力竭后即刻
骨骼肌组织内Na+-K+ATPase活性,运动+热适应组显著高于对照组(P<0.05)；运动+热适应+Vc组极显著高于对照组和运动组(P<0.01)；运动+热适应+Vc组显著高于运动+热适应组(P<0.05)。血清组织内CK活性,运动组、运动+热适应、运动+热适应+Vc组极显著低于对照组(p<0.01)。血清组织中LD含量、LDH活性,没有显著性差异；(P>0.05)；SDH活性运动+热适应组显著高于对照组(p<0.05)；运+热+VC极显著高于对照组(P<0.01)。
4结论
4.1热适应1周和Vc可以显著提高热应激大鼠Na+-K+-ATPase活性,特别是热适应和Vc联合作用下可以极显著提高Na+-K+-ATPase、SDH活性,说明热适应1周和Vc联合作用对热应激大鼠有氧代谢能力的提高具有极显著的效果。
4.2安静状态下,热适应1周的热应激大鼠血清CK活性显著升高,在定量负荷和力竭状态下CK活性升高不明显,说明热环境对机体能造成损害,但热适应—周可以降低这种损伤。
4.3 Vc是强还原剂具有清除自由基保护机体的作用,可以显著提高热应激大鼠的代谢能力,但是无法防御高温环境下的机体损伤。热适应1周防御高温环境对热应激大鼠损伤,但提高大鼠代谢能力的效果比Vc差。因此只有热适应和Vc共同作用才能显著提高热应激大鼠的能量代谢及降低损伤。
1 Objective:
By observing the thermal adaptation and energy metabolism in rats taking Vc related indicators on the impact of heat adaptation, Vc energy metabolism in rats and its mechanism in order to enhance sport athlete in high temperature environment, ability to provide a theoretical basis.
2 Methods
8-week-old Wistar rats were 108, weight 200±15g, were randomly divided into 4 groups:A (normal control group), B (exercise group), C (exercise+heat acclimation group), D (exercise+heat adaptation+Vc group); B, C, D rats were generally treadmill training for 5 weeks,6 weeks on the C, D groups heat adaptation (38℃) trained and fed to the D group Vc, increasing exercise training hours per week:1 week of training time as 10min, speed 10m/min; weekly time increments after 10min, the speed increase 3m; the first week of training time for the 4,5,6 40min, speed of 21m/min. By resting state, fixed load condition, state of exhaustion, immediately after exercise were killed, skeletal muscle, serum, measured quadriceps, gastrocnemius muscle tissue Na+-K+ATPase, CK, LD, LDH, SDH activity. SPSS 10.0 statistical software with all the data analysis, the significant level of p<0.05.
3 results
3.1 resting state Within the skeletal muscle Na+-K+ATPase activity, exercise+heat adaptation was significantly higher (P<0.01); movement adaptation+Vc+heat group was significantly higher than the other 3 groups (P<0.01); serum tissue in CK activity, motion and heat adaptation was significantly higher (P<0.05); movement adaptation+Vc+heat group was significantly higher (P<0.05); serum levels within the organization LD, LDH activity was not significantly different (P> 0.05); SDH activity exercise+heat adaptation was significantly higher; movement adaptation+ Vc+heat group was significantly higher than the other 3 groups (P<0.01).
3.2 Quantitative exercise 1 hour Within the skeletal muscle Na+-K+ATPase activity, exercise+heat adaptation was significantly higher (P<0.01); movement adaptation+ Vc+heat group was significantly higher than the other 3 groups (P<0.01); serum tissue CK activity in the exercise group, exercise+heat adaptation was significantly lower than the control group (p<0.05). Serum levels within the organization LD, LDH activity were not significantly different; (P> 0.05); SDH content exercise+heat adaptation was significantly higher (p<0.05); Games+hot+VC was significantly higher and the movement group (P<0.01).
3.3 immediately after exhaustive Within the skeletal muscle Na+-K+ATPase activity, exercise+heat adaptation was significantly higher (P<0.05); movement adaptation+Vc+heat group was significantly higher and the exercise group (P <0.01); Sports+heat adaptation+Vc was significantly higher than the exercise+ heat acclimation group (P<0.05). Serum CK activity within the organization, sports group, motion and heat adaptation, motion and heat adaptation+Vc group was significantly lower than the control group (p<0.01). Tissue content of serum LD, LDH activity, no significant difference; (P> 0.05); SDH activity exercise+heat adaptation was significantly higher (p<0.05); Games+hot+VC was significantly higher (P<0.01).
4 Conclusion
4.1 Thermal adaptation and 1 week and Vc can significantly improve the heat stress in rats Na+-K+-ATPase activity, in particular, thermal adaptation and Vc combined effect may be significantly increased Na+-K+-ATPase, SDH activity, indicating thermal adaptation 1 week and Vc combined effects of heat stress to improve aerobic capacity in rats with highly significant results.
4.2 at rest, heat 1 week to adapt to the heat stress in rats significantly increased serum CK activity in the quantitative load and CK activity increased after exhaustive state is not obvious, indicating the thermal environment of the body can cause damage, but heat adaptation week can reduce this damage.
4.3 Vc is a strong reducing agent has a clear role of free radicals protecting the body, but can not defense the body under high temperature damage.
引文
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