头穴丛刺结合环境刺激对缺氧缺血性脑损伤新生大鼠神经可塑性影响的实验研究
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摘要
目的观察头穴丛刺结合环境刺激对缺氧缺血性脑损伤新生大鼠海马微管相关蛋白-2和突触素表达及突触超微结构等的影响,探讨头穴丛刺结合环境刺激对新生大鼠缺氧缺血性脑损伤后神经可塑性影响的物质基础及其发生机制。方法制作HIBD新生动物模型,随机分为五组。采用Morris水迷宫检测学习记忆能力、免疫组化检测各组海马CA1区MAP-2和突触素的表达、透射电镜观察神经元突触超微结构和突触数目。结果(1)头穴丛刺结合环境刺激法能够促进缺氧缺血性脑损伤大鼠学习记忆能力的提高;(2)头穴丛刺结合环境刺激法使海马CA1区MAP-2的表达增高;(3)头穴丛刺结合环境刺激法能够增加海马CA1区突触素的表达;(4)头穴丛刺结合环境刺激法可以减轻HI所致的突触超微结构损伤,改善突触结构神经信息的传递,促进缺血缺氧的脑组织超微结构恢复;(5)头穴丛刺结合环境刺激法可促进突触数目的增加。结论头穴丛刺结合环境刺激法能够促进缺氧缺血性脑损伤大鼠学习记忆能力的提高;海马CA1区MAP-2和突触素表达的增高,缺血缺氧的脑组织超微结构恢复和突触数目的增加,可能参与了头穴丛刺与环境刺激对HIBD神经可塑性的影响机制。
Objective To observe the first hole cluster-needling combined environmental stimuli on hypoxic-ischemic brain injury in neonatal rats with hippocampal microtubule-associated protein -2 and synaptophysin expression and synaptic ultrastructure of the impact of the first points of the integration of environmental stimulation on the cluster-needling hypoxic-ischemic brain injury affect the material basis of neural plasticity and its mechanism. Methods Of production of HIBD newborn animals were randomly divided into five groups. Using Morris water maze test of learning and memory capacity of each group was detected by immunohistochemistry in hippocampal CA1 area MAP-2 and synaptophysin expression, transmission electron microscopy observation of neuronal synapses and synaptic ultrastructure number. Results (1) the first hole cluster-needling the integration of environmental stimulation can contribute to hypoxic-ischemic brain injury in rats with learning and memory capabilities; (2) the first hole cluster-needling the integration of environmental stimulation of hippocampal CA1 area increased expression of MAP-2; (3) the first hole cluster-needling combined environmental stimuli can increase hippocampal synaptophysin expression in CA1 area; (4) the first hole cluster-needling combined environmental stimuli can reduce HI-induced synaptic ultrastructure injury, to improve the synaptic structure neural transmission of information to promote the hypoxic-ischemic brain tissue ultrastructure recovery; (5) the first hole cluster-needling combined environmental stimuli can promote an increase in the number of synapses. Conclusion Of scalp cluster-needling combined environmental stimulation can contribute to hypoxic-ischemic brain injury in rats with learning and memory capabilities; hippocampal CA1 area MAP-2 and synaptophysin expression increased in hypoxic-ischemic brain ultrastructure to resume and the increase in the number of synapses may be involved in the first cluster-needling acupoint stimulation on HIBD and environmental impact of neural plasticity mechanisms.
Objective To observe the first hole cluster-needling combined environmental stimuli on hypoxic-ischemic brain injury in neonatal rats with hippocampal microtubule-associated protein -2 and synaptophysin expression and synaptic ultrastructure of the impact of the first points of the integration of environmental stimulation on the cluster-needling hypoxic-ischemic brain injury affect the material basis of neural plasticity and its mechanism. Methods Of production of HIBD newborn animals were randomly divided into five groups. Using Morris water maze test of learning and memory capacity of each group was detected by immunohistochemistry in hippocampal CA1 area MAP-2 and synaptophysin expression, transmission electron microscopy observation of neuronal synapses and synaptic ultrastructure number. Results (1) the first hole cluster-needling the integration of environmental stimulation can contribute to hypoxic-ischemic brain injury in rats with learning and memory capabilities; (2) the first hole cluster-needling the integration of environmental stimulation of hippocampal CA1 area increased expression of MAP-2; (3) the first hole cluster-needling combined environmental stimuli can increase hippocampal synaptophysin expression in CA1 area; (4) the first hole cluster-needling combined environmental stimuli can reduce HI-induced synaptic ultrastructure injury, to improve the synaptic structure neural transmission of information to promote the hypoxic-ischemic brain tissue ultrastructure recovery; (5) the first hole cluster-needling combined environmental stimuli can promote an increase in the number of synapses. Conclusion Of scalp cluster-needling combined environmental stimulation can contribute to hypoxic-ischemic brain injury in rats with learning and memory capabilities; hippocampal CA1 area MAP-2 and synaptophysin expression increased in hypoxic-ischemic brain ultrastructure to resume and the increase in the number of synapses may be involved in the first cluster-needling acupoint stimulation on HIBD and environmental impact of neural plasticity mechanisms.
引文
[1]黄茂.中医学对脑瘫的病因病机认识及综合治疗探讨。河北中医药学报,2009,24(1):10-11
[2]李树春.小儿脑性瘫痪[M]。郑州:河南科学技术出版社,2000:168
[3]王军英。中医辨证分型治疗小儿脑性瘫痪[J]。现代康复,2001,5(8):44
[4]雷正荣.史方奇治疗小儿脑性瘫痪经验[J].实用中医药杂志,1994,10(2):5-6
[5]卢映.中药治疗小儿脑瘫22例临床观察[J].中国优生与遗传杂志,1996,12(4):70
[6]黄茂,魏民,范焕芳。综合疗法治疗小儿脑瘫临床分析。河北中医,2005,27(10):743-745
[7]李晓霞,梁素霞,杨冬梅.按摩疗法治疗超早期小儿脑性瘫痪[J].中国临床康复, 2003,7(18):2620
[8]米曙光.头针滞针法治疗小儿脑瘫临床观察[J].中国针灸,2002,22(7):461
[9]张小莉,王祥荣,张秀宝,等.针刺夹脊为主治疗小儿脑瘫354例疗效观察[J].中国针灸,2000,(5):269
[10]张海婷,杨文卓,孙宗芝,等.头针治疗小儿脑瘫100例疗效观察[J].中国中医药信息杂志.2003,10(1):63-64
[11]谢安树,刘立席.中医治疗小儿脑瘫26例小结[J].四川中医. 2003, 21(10) :77
[12]韩雪.头针治疗小儿脑性瘫痪的康复疗效[J].中国临床康复,2002,6(5):748
[13]Saffran M,Sudesh G,SavariarC,et at.A Mew ap-proach to the oraladminstration of onsrlin and other peptidedrugs[J].science,1987,233:1081-1084
[14]张忠芳,马骏,彭娟.胎儿宫内窘迫的产科原因分析「J].工企医刊,2003; 16(6):19
[15]王辉.国内脑瘫的研究近况〔J〕.中国康复医学杂志,2004,19(8):637-640
[16]胡莹媛.小儿脑性瘫痪的康复[J].中国康复理论与实践,2003,9(4):193-195
[17]赵聪敏.围生期缺氧缺血性脑损伤的治疗进展[J].中国医药指南, 2008, 6(12):17-18
[18]A chur.Neuroprotection against ischemic/hypoxic brain damage:blockersof ionotropic glutamate receptor and voltage sensitive calcium channels.Curr Drug Targets.2004;5(7):603-618
[19]Teragawa H,Matsuura H,Chayama K,et al.Mechanisms responsible for vasodilation upon magnesium infusion in vivo:clinical evidence.Magnes Res.2002;15(3-4):241-246
[20]苏闻,罗盛,蔡葵等,氟桂利嗪对急性缺血性脑梗死缺血半暗带组织的影响.中国临床康复.2004;8(31):6948-6949
[21]王静敏,董丽媛,郝翼中等,尼莫地平对新生大鼠缺氧缺血性脑病的防治作用.山西医科大学学报,2002;33(1):17-19
[22]Coert BA,Anderson RE,Meyer FB.Is neuroprotective efficacy of nNOS inhibitor7-NI dependant on ischemic in tracellular PH?Am J Physiol Heart Circ Physiol.2003;284(1):H 151-159
[23]Sugimoto K,Ladcola C.Effects of aminoguanidine on cerebral ischemia in mice:comparison between mice with and without inducible nitric oxide synthase gene.Nueurosci Lett.2002;331(1):25-28
[24]Drummond JC,Mckay LD,Coled J,et al.The role of nitric oxide synthase inhibition in the adverse effects of etomidate in the setting of focal cerebral ischemia in rats.Anesth Analg.2005;100(3):841-846
[25]Abdullah Kumral,Huseyin Baskin,Necati Gokmen,et al.Selective inhibition of nitricoxide in hypoxic-ischemic brain model in newborn rats:is it an explanation for theprotective role of erythropoietin?Biol Neonate.2004;85(1):51-54
[26]Feng Y,Piletz JE,Leblanc MH,et al.Agmatine suppresses nitric oxide production and attenuates hypoxic-ischemic brain injury in neonatal rats.Pediatr Res.2002;52(4):606-611
[27]Avital Schurr.Lactate,glucose and energy metabolism in the ischemic brain.Molecular medic.2002;10(3):131-136
[28]孙桂莲,胡宛如.BFGF对新生大鼠缺氧缺血后海马CA1区神经细胞凋亡的影响.新生儿杂志.2001;16(4):162-163
[29]Sugimorl M,Speller M,Finklestein SP.Intravenous basic fibroblastgrowth factorproduces apersistent reduction in infarct volume following permanent focal ischemia in rats.Neurosci Lett.2001;300(1):13-16
[30]Himmelseher S,P Fenninger E,Geo Rgieff M.Effect of basic fibroblast growth factoron hippocampal neurons after axonal injury.Trauma.1997;42(5):659-664
[31]Leeds P,Leng Y,Chalecka Franaszek E et al.Neurotrophins protect against cytosine arabinoside-induced apoptosis of immature rat cerebellar neurons.Neurochem Int.2005;46(1):61-72
[32]胡伟,赵聪敏,黄绍平.神经节苷脂对新生鼠缺氧缺血性脑损伤的保护作用.中国儿童保健杂志.2005;13(2):131-133
[33]Akisu M,Huseyinov A,Yalaz M,et al.Selective head cooling with hypothermia suppresses the generation of platelet-activating factor in cerebrospinal fluid of newborn infants with perinatal asphyxia.Prostaglandins-Leukot-Essent-Fatty-Acids.2003;69(1):45-50
[34] Zhu CL,Wang X Y,Cheng X Y,et al.Neuroprotective effect and mechanisms ofhypothermia in neonatal rat cerebral hypoxic-ischemic damages.Zhonghua Er Ke ZaZhi.2003;41(12):911-915
[35] Ren H,Wang W,Ge Z,et al,Clinical,brain electric earth map,endothelin and transcranialul transonic Doppler findings after hyerbaric oxygen treatment for severebrain injury.Chin Med J(Engl).2001;114(4):387-390
[36]Palisano RJ.Concurrent and predicti vevalidities of the Bayley Motor Scale and the Peabody Developmental Motor Scales[J].Phys Ther,1986,66(11):1714-1719
[37]Provost B,CroweTK,McClain C.Concur-rent validity of the Bayley Scales of Infant Development II Motor Scale and the Pea-body Developmental Motor Scales in two-year-old children[J].Phys Occup Ther Pediatr,2000,20(1):5-18
[38]孟晓慧,刘静,朱志萍.蜡疗对187例脑性瘫痪患儿肌张力和关节活动度的作用[J].中国临床康复,2004,8(15):2947
[39]陆华宝.脑瘫儿童的作业治疗[J].中国康复理论与实践, 2003 ,9(4):198-200
[40]刘欣,张长杰编译.小儿脑瘫的研究近况[J].国外医学:物理医学与康复学分册,2004 ,24 (4):145-147
[41]中华人民共和国卫生部医政司.中国康复医学诊疗规范:上册[M].北京:华夏出版社,1999:157-162
[42]于炎冰,张黎,伍成奇,等.显微神经外科手术治疗痉挛型脑瘫738例临床观察〔J〕.中华神经外科杂志,2004,20(1):59-62
[43]刘仰兵,赵永振.新生儿缺氧缺血性脑病的防治进展〔J〕.临床误诊误治,2005,18(11):778-779
[44]王杨,王琍琍,步仰高,等. 230例新生儿缺氧缺血性脑病10年回顾性调查及围产因素分析[J].中国全科医学,2004, 7(17): 1232-1233.
[45]刘映粼.高危妊娠监护治疗学[M].北京:北京医科大学·中国协和医科大学联合出版社, 1998. 99.
[46]陈长庚,胡旭红.新生儿窒息复苏及HIE发病机理新进展[J].四川卫生管理干部学院学报, 1999, 18(3): 95-96.
[47]孟娥,头针配合穴位注射早期治疗小儿缺氧缺血性脑病预防脑瘫.四川中医,2003,21(11):92一93
[48]景玉宏,王子仁.电针“百会”穴对大鼠记忆保持能力的影响及海马区ofs蛋白的表达.针刺研究,2004,6(2):107一110]
[49]何竟,黄长琼,张永玲等,针灸治疗小儿脑性瘫痪的临床文献质量评价和思考.中国针灸,2002,22(10):704一706
[50]吴美倩,张亦波.针刺对小儿脑瘫作用机制的初步探讨[J].江苏临床医学杂志,1998,6(7):464-467
[51]刘振寰,张鸿雁.头针为主治疗小儿脑瘫210例的临床研究[J].中国针灸,1999,19(1):651-653
[52]梁松,吴洋,高春霞,等.头针治疗小儿脑性瘫痪104例疗效分析[J].现代中西医结合杂志,2004,13(1):60-62
[53]厉华,水泉祥.小儿脑瘫[M].北京:北京医科大学/中国协和医科大学联合出版社,1996:119-149
[54]于海波,靳瑞.针刺对小儿脑瘫患者诱发肌电图的即时影响[J].中国针灸,1998,18(11):6-7
[55]施丙培,卜怀娣,季志英,等.针灸治疗对脑性瘫痪及精神发育迟缓患儿生长激素水平的影响[J].中国临床康复,2004,8(30):6627-6631
[56]施丙培,卜怀娣,赵瑞芳,等.针刺对小儿脑病骨钙素影响的观察[J].上海针灸杂志,2004,23(12):18-19
[57]丁春华,刘焕荣,张少丹,等.针灸治疗窒息脑瘫幼鼠的实验研究[J].河北医科大学学报,2000,21(3):155-156
[58]杜元灏,翟娜,李谈,等.针刺对脑梗塞模型大鼠脑传导纤维及尼氏体的影响.针灸临床杂志,1998,14:43-44.
[59]宛青,刘伟,吴爱群,等.电针对新生大鼠缺血缺氧后脑组织ChAT和BDNF表达的影响.解剖学研究,2003,25(4);295-297
[60]Mattson MP,Lovell MA,Furukawa K,et al.Neurotrophic factors attenuate glutamate-induced accumulation of peroxides,elevation of intracellular Ca2+ concentration, and neurotoxicity andincrease antioxidant enzyme activities in hippocampal neurons.JNeurochem,1995,65:1740-1751.
[61]Almli CR,levy TJ,Han BH,et al.BDNF protects against spatial memory deficits following neonatal hypoxia-ischemia. Exp Neurol,2000,166:99-114.
[62]Chen J,Simon R.Ischemic tolerance in the brain [J].Neurol,1997(2):306.
[63]Frim Dm,Short MP,Rosenbery WS,et al.Local protective effects of nerve growth factor secreting fibroblasts against excitotoxic le-sions in the rat striatum[J].J Neurosurg,1993,(2):267-273.
[64]Holtzm an DM,Sheldon RA,Jaffe W,et al.Nerve growth factor protects the neonatal brain against hypoxic- ischemic injury[J].Ann Neurol,1996,39(1):114-122.
[65]闫丙川,刘合玉,贾天明,等.针刺对缺氧缺血性脑损伤大鼠保护作用的实验研究.神经疾病与精神卫生,2005,5(1):33-35
[66]Nakatomi H, Kuriu T,Okabe S,et al.Regeneration of hippocampal pyramidal neurons after ischemic brain injury by recruitment of en-dogenousneural progenitors[J].Cell,2002,110(4):429-441.
[67]曾光伟,张伟宏,吴爱群,等。电针对缺血缺氧新生大鼠脑内神经前体细胞增殖的影响.郑州大学学报(医学版),2004,39(6):977-979
[68]Pham TM, W inblad B, Granholm AC, et al Environmental in-fluences on brain neurotrophins in rats. Pharmacol Biochem Be-hav, 2002, 73: 167-175.
[69]BRUEL-JUNGERMAN E, LAROCHE S, RAMPON C. New neurons in the dentate gyrus are involved in the expression of enhanced long-term memory following environmental enrichment[J]. Eur JNeurosi, 2005, 21(2): 513-521.
[70]SCHRIDDE U, VAN-LUIJTELAAR G. The role of the environmenton the development of spike-wave discharges in two strains of rats[J].PhysiolBehav, 2005, 84 (3): 379-386.
[71]PABAN V,JAFFARDM, CHAMBON C,etal T ime course ofbehav-ioral changes following basal forebrain cholinergic damage in rats:En-vironmental enrichment as a therapeutic intervention [J]. Neuro-science, 2005, 132(1): 13-32.
[72]鲁利群,赵聪敏,蒲昭霞.环境刺激对缺氧缺血性脑损伤大鼠海马巢蛋白表达及学习记忆的影响.第三军医大学学报,2006,28(8):804-806
[73]鲁利群,范建义,赵聪敏.环境刺激对缺氧缺血性脑损伤新生大鼠学习记忆及海马病理学的影响.实用儿科临床杂志,2007,22(2):103-105
[74]GobboOL, O′Mara SM. Impact of enriched-environment housing onbrain-derived neurotrophic factor and on cognitive performance after a transientglobal ischem ia[J].B ehav B rain Res,2004,152:231-241.
[75]Singer W. Development and plasticity of cortical processing architecture, Science, 1995,270:758-764.
[76]Abrahm IM, Kovcs KJ. Postnatalhandling alters the activation of stress-related neuronal circuitries.Eur JNeurosci,2000, 12:3003-3014.
[77]Yi Li,Ning Jiang, Cecylia Powers. Neurodamage and plasticity identified by MAP2, GAP43, CyclinDI immunoreactivity after fo-cal cerebral ischemia in rats. Stroke, 1998, 29: 1972-1980.
[78]李雪梅,蒲昭霞,周晓军。环境刺激对缺氧缺血性脑损伤新生大鼠海马微管相关蛋白-2表达的影响,中国新生儿科杂志,2008,23(1)31-34
[79]Stroemer RP,Kent TA,Hulsebosch CE.Neocortical neural sprouting,synaptogenesis,and behavioral recovery after neocortical infarction inrats.Stroke 1995;26:2135
[80]Schlaepfer WW.Neurofilament:structure,metabolism and implication in disease.J Neuropathol Exp Neurol 1987;46(3):117
[81]Briones TL,Woods J,Wadowska M,et al.Astrocytic changes in the hippocampus and functional recovery after cerebral ischemia are facilitated by rehabilitation training.Behavioural Brain Res 2006;171:17-25
[82]Matsumori Y,Hong SM,Fan Y,et al.Enriched environment and spatial learning enhance hippocampal neurogenesis and salvages ischemic penumbra after focal cerebral ischemia.Neurobiol Dis 2006;22(1):187-198
[83]Sonninen R,Virtanen T,Sivenius J,et al.Gene expression profiling in the hippocampus of rats subjected to focal cerebral ischemia and enriched environment housing.Restor Neurol Neurosci 2006;24(1):17-23
[84]Gama SM,Friedrich V,DegasperirR,et al.Human midsized neurofilament subunit induces motor neuron disease in transgenic mice.Exp Neurol 2004;184(l):408-419
[85]Pal J,Toth Z,Farkas O,et al.Selective induction of ultrastructural(neurofilament)compaction in axons by means of a new head-injury apparatus.Neurosci Methods 2006;153(2):283-289
[86]Letournel F,Bocquet A,Perrot R,et al.Neurofilament high molecular weight-green fluorescent protein fusion is normally expressed in neurons and transported in axons:a neuronal marker to investigate the biology of neurofilaments.Neuroscience 2006;137(1):103-111
[87]AL Chalabt A,Miller CC.Neurofilaments and neurological disease[J].Bioessays 2003;25(4):34
[88]Stanley EF.Presynaptic calcium channels and the depletion of synaptic cleft calcium.J Neurophysiol 2000;83(1):477-82
[89]于若谷,赵聪敏,王丽雁,等.丰富环境刺激对缺氧缺血性脑损伤大鼠脑超微结构改变及神经丝蛋白表达的影响.中国组织工程研究与临床康复,2007,11(13):2503-2506
[90]WHISHAW IQ, RODM R, AUER R N. Behavioral deficits revealed bymultiple tests in ratswith ischemic damage limited to halfof the CA1 sector of the hippocampus[J]. Brain ResBull 1994, 34(3): 283-289
[91]THIEL G. Synapsin I, synapsin II, and synaptophysin: marker pro-teins of synaptic vesicles [J]. Brain Pathol 1993, 3(1): 87-95
[92]蒲昭霞,赵聪敏,鲁利群,等.丰富环境对缺氧缺血性脑损伤大鼠海马突触超微结构及突触素表达的影响.第三军医大学学报,2006,28(8)816-818
[93]FernandazV, Adaro L, Sanhueza-TsutsumiM, et al Early-lifepoly sensorial stimulation and nutrition: topographic levels of sus-ceptibility in the rat visual cortex. BiolNeonate, 1997, 71: 265-276
[94]华兴邦.大鼠穴位图谱的研制.实验动物与动物实验,1991,(1):1-5
[95]Rosenberg AA,Muydaugh E.The effect of blood glucogse consentration on postasphyxia cerebal hemodynamics in newborn lambs.Pediatr Res ,1990;27:454
[96]John W.Calvert , Changman Zhou , et al.Zhang Hyperbaric oxygenation prevented brain injury induced by hypoxia-ischemia in a neonatal rat model[J].Brain Res,2002,951(l):l-8
[97]姚裕家,李炜如。地塞米松在新生猪缺氧缺血性脑损伤中的防治作用。中华儿科杂志,1994,32(4):201一202
[98]Vannucci RC,Vannucci SJ.A model of Perinatal hypoxic-ischemic brain damage[J].Ann NY Acad Sci,1997,835:234-249
[99]Riee J E,Vannucci RC,Brierley JB,etal.The influence of immaturity on hypoxic-ischemic brain damage in the rat[J].Ann Neurol , 1981 ,9:131-141
[100]Gilland E, Hagherg H.NMDA Receptor-dependent increase of perebral glucose utilization after hypoxia-ischemia in the immature rat.J CerebBlood Flow Metab.1996,16(5):1005-1013
[101]Vanucci RC,YagerJY,Vannucci SJ.Regional cerebral blood flow during hypoxia-ischemia in immature rats.Stroke,1988,19(2):245-250
[102]Romijn HJ, Homfan MA, Gramsbergen A.At what age is the developing cerebral cortex of the rat comparable to that of the full-term neubom human baby?EaHy Hum Dev,1991,26(1):61-67
[103]Hu BR,Liu CL,Ou yang Y,et al.Invol vement of caspase-3 in cell death after hypoxia-ischemia declines during brain maturation.J cered Blood Flow Metab,2000,20(9):1294-1300
[104]Muse ED, Jurevics H, Toews AD, et al.Parameters related to lipid metabolism as markers of myelination in mouse brain J Neurochem ,20()1;76(1):77-86
[105]Ginsberg MD,Busto R.Rodent models of cerebral isehemia.Storke,1989,20(12):1627-1642
[106]Vannuci RC,Vannucci SJ.Perinatal hypoxic-isehemic brain damage:evolution of an animal model.Dev Neurosci,2005,27(2-4):81-86
[107]汤健,张洪梅,吕静.小儿脑瘫头针治疗进展.南京中医药大学学报,2008,24(5):355-357
[108]孙忠人,霍立光,刘丽莉等.头皮针刺运动诱发电位的研究.中国中医药科技,1994,(16):14- 17
[109]唐强.头穴丛刺结合易化技术对脑卒中康复治疗作用的研究.博士后研究工作报告,2001,11-67
[110]早期干预降低早产儿脑性瘫痪发生率研究协作组.降低早产儿脑性瘫痪发生率的临床研究[J]。中华儿科杂志,2005,43(4):244一246
[111]Yager JY , Wright S , Armstrong EA,et al.Theinfluence of aging on recovery following isehemic brain damage[J].Behav Brain Res,2006,173(2):171-180
[112]Pham TM, W inb lad B, Granholm AC, et al Environmental in-fluences on brain neurotrophins in rats. Pharmacol Biochem Be-hav, 2002, 73: 167-175
[113]Markwoska AL, Spangler EL.Behavioral assessment of the senescence accelerated moues(SAM P8 and Rl),Physiol.Behav,1998,64:15一26
[114]Arteni NS , Salgueiro J.Neonatal cerebral hypoxia ischemia causes lateralized memory impairments in the adult rat.Brain Research,2003,973(2):171一178
[115]Abraham M, Kovacs KJ. Postnatal hand ling alters the activation of stress-related neuronal circuitries. Eur JN eurosci,2000, 12:3003-3014.
[116]Cristofan illiM, Thanos S, Brosius J, et al .Neuronal MAP2 mR-NA: species-dependent differential dendritic targeting compe-tence. JMol Biol, 2004, 341: 927-943
[117]Yi Li,Ning Jiang,Cecylia Powers.Neurodamage and plasticity identified by MAP2, GAP43, CyclinDI imm unoreactivity after fo-cal cerebral ischemia in rats. Stroke, 1998, 29: 1972-1980
[118]Daly C, Sugimori M, Moreira JE. Synaptophysin regulates clathrin independent endocytosis of synaptic vesicles[J].Proc Natl Acad,2000,97:6120-6125
[119]Leypoldt F, Flajolet M, Methner A. Neuronal differentiation of cultured human Ntera-2cl, DI cells leads to increased expression of synapsins[J]. Neurosci Lett,2002,324:37-40
[120]Ferreira A, Han HQ, Greegnard P, et al. Suppression of synapsin II inhibits the formation ad maintenance of synap- ses in hippocampal culture[J]. Proc Natl Acad Sci USA, 1995,92:9225-9229
[121]Mullany PM, Lynch MA. Evidence for a role for synato-physin in expression of long-term potentiation I rat dentate gyrus[J]. Neuroreport, 1998, 9:2489-2491
[122]蒲昭霞,赵聪敏,李亚伶等.脑发育不同阶段丰富环境刺激对大鼠海马突触素表达的影响.中国儿童保健杂志,2007,15(6):632-634
[123]宋文秀,曹云涛,刘华庆.新生鼠脑缺血再灌注后海马CA1区突触素的表达及意义[J].第四军医大学学报,2004,25(16):1467-1469
[124]WHISHAW IQ, RODM R, AUER R N. Behavioral deficits revealed by multipletests in ratswith ischemic damage limited to halfof theCA1 sector of the hippocampus [J]. Brain ResBull, 1994, 34(3): 283-289
[125]THIEL G. Synapsin I, synapsin II, and synaptophysin: marker pro-teins of synaptic vesicles [J]. Brain Pathol, 1993, 3(1): 87-95
[126]田春海,周丛乐,苗鸿才.新生大鼠缺氧缺血后远期海马CA1区神经元和突触的变化及干预的效果[J].中华围产医学杂志,1999, 2(1): 33-35
[2]李树春.小儿脑性瘫痪[M]。郑州:河南科学技术出版社,2000:168
[3]王军英。中医辨证分型治疗小儿脑性瘫痪[J]。现代康复,2001,5(8):44
[4]雷正荣.史方奇治疗小儿脑性瘫痪经验[J].实用中医药杂志,1994,10(2):5-6
[5]卢映.中药治疗小儿脑瘫22例临床观察[J].中国优生与遗传杂志,1996,12(4):70
[6]黄茂,魏民,范焕芳。综合疗法治疗小儿脑瘫临床分析。河北中医,2005,27(10):743-745
[7]李晓霞,梁素霞,杨冬梅.按摩疗法治疗超早期小儿脑性瘫痪[J].中国临床康复, 2003,7(18):2620
[8]米曙光.头针滞针法治疗小儿脑瘫临床观察[J].中国针灸,2002,22(7):461
[9]张小莉,王祥荣,张秀宝,等.针刺夹脊为主治疗小儿脑瘫354例疗效观察[J].中国针灸,2000,(5):269
[10]张海婷,杨文卓,孙宗芝,等.头针治疗小儿脑瘫100例疗效观察[J].中国中医药信息杂志.2003,10(1):63-64
[11]谢安树,刘立席.中医治疗小儿脑瘫26例小结[J].四川中医. 2003, 21(10) :77
[12]韩雪.头针治疗小儿脑性瘫痪的康复疗效[J].中国临床康复,2002,6(5):748
[13]Saffran M,Sudesh G,SavariarC,et at.A Mew ap-proach to the oraladminstration of onsrlin and other peptidedrugs[J].science,1987,233:1081-1084
[14]张忠芳,马骏,彭娟.胎儿宫内窘迫的产科原因分析「J].工企医刊,2003; 16(6):19
[15]王辉.国内脑瘫的研究近况〔J〕.中国康复医学杂志,2004,19(8):637-640
[16]胡莹媛.小儿脑性瘫痪的康复[J].中国康复理论与实践,2003,9(4):193-195
[17]赵聪敏.围生期缺氧缺血性脑损伤的治疗进展[J].中国医药指南, 2008, 6(12):17-18
[18]A chur.Neuroprotection against ischemic/hypoxic brain damage:blockersof ionotropic glutamate receptor and voltage sensitive calcium channels.Curr Drug Targets.2004;5(7):603-618
[19]Teragawa H,Matsuura H,Chayama K,et al.Mechanisms responsible for vasodilation upon magnesium infusion in vivo:clinical evidence.Magnes Res.2002;15(3-4):241-246
[20]苏闻,罗盛,蔡葵等,氟桂利嗪对急性缺血性脑梗死缺血半暗带组织的影响.中国临床康复.2004;8(31):6948-6949
[21]王静敏,董丽媛,郝翼中等,尼莫地平对新生大鼠缺氧缺血性脑病的防治作用.山西医科大学学报,2002;33(1):17-19
[22]Coert BA,Anderson RE,Meyer FB.Is neuroprotective efficacy of nNOS inhibitor7-NI dependant on ischemic in tracellular PH?Am J Physiol Heart Circ Physiol.2003;284(1):H 151-159
[23]Sugimoto K,Ladcola C.Effects of aminoguanidine on cerebral ischemia in mice:comparison between mice with and without inducible nitric oxide synthase gene.Nueurosci Lett.2002;331(1):25-28
[24]Drummond JC,Mckay LD,Coled J,et al.The role of nitric oxide synthase inhibition in the adverse effects of etomidate in the setting of focal cerebral ischemia in rats.Anesth Analg.2005;100(3):841-846
[25]Abdullah Kumral,Huseyin Baskin,Necati Gokmen,et al.Selective inhibition of nitricoxide in hypoxic-ischemic brain model in newborn rats:is it an explanation for theprotective role of erythropoietin?Biol Neonate.2004;85(1):51-54
[26]Feng Y,Piletz JE,Leblanc MH,et al.Agmatine suppresses nitric oxide production and attenuates hypoxic-ischemic brain injury in neonatal rats.Pediatr Res.2002;52(4):606-611
[27]Avital Schurr.Lactate,glucose and energy metabolism in the ischemic brain.Molecular medic.2002;10(3):131-136
[28]孙桂莲,胡宛如.BFGF对新生大鼠缺氧缺血后海马CA1区神经细胞凋亡的影响.新生儿杂志.2001;16(4):162-163
[29]Sugimorl M,Speller M,Finklestein SP.Intravenous basic fibroblastgrowth factorproduces apersistent reduction in infarct volume following permanent focal ischemia in rats.Neurosci Lett.2001;300(1):13-16
[30]Himmelseher S,P Fenninger E,Geo Rgieff M.Effect of basic fibroblast growth factoron hippocampal neurons after axonal injury.Trauma.1997;42(5):659-664
[31]Leeds P,Leng Y,Chalecka Franaszek E et al.Neurotrophins protect against cytosine arabinoside-induced apoptosis of immature rat cerebellar neurons.Neurochem Int.2005;46(1):61-72
[32]胡伟,赵聪敏,黄绍平.神经节苷脂对新生鼠缺氧缺血性脑损伤的保护作用.中国儿童保健杂志.2005;13(2):131-133
[33]Akisu M,Huseyinov A,Yalaz M,et al.Selective head cooling with hypothermia suppresses the generation of platelet-activating factor in cerebrospinal fluid of newborn infants with perinatal asphyxia.Prostaglandins-Leukot-Essent-Fatty-Acids.2003;69(1):45-50
[34] Zhu CL,Wang X Y,Cheng X Y,et al.Neuroprotective effect and mechanisms ofhypothermia in neonatal rat cerebral hypoxic-ischemic damages.Zhonghua Er Ke ZaZhi.2003;41(12):911-915
[35] Ren H,Wang W,Ge Z,et al,Clinical,brain electric earth map,endothelin and transcranialul transonic Doppler findings after hyerbaric oxygen treatment for severebrain injury.Chin Med J(Engl).2001;114(4):387-390
[36]Palisano RJ.Concurrent and predicti vevalidities of the Bayley Motor Scale and the Peabody Developmental Motor Scales[J].Phys Ther,1986,66(11):1714-1719
[37]Provost B,CroweTK,McClain C.Concur-rent validity of the Bayley Scales of Infant Development II Motor Scale and the Pea-body Developmental Motor Scales in two-year-old children[J].Phys Occup Ther Pediatr,2000,20(1):5-18
[38]孟晓慧,刘静,朱志萍.蜡疗对187例脑性瘫痪患儿肌张力和关节活动度的作用[J].中国临床康复,2004,8(15):2947
[39]陆华宝.脑瘫儿童的作业治疗[J].中国康复理论与实践, 2003 ,9(4):198-200
[40]刘欣,张长杰编译.小儿脑瘫的研究近况[J].国外医学:物理医学与康复学分册,2004 ,24 (4):145-147
[41]中华人民共和国卫生部医政司.中国康复医学诊疗规范:上册[M].北京:华夏出版社,1999:157-162
[42]于炎冰,张黎,伍成奇,等.显微神经外科手术治疗痉挛型脑瘫738例临床观察〔J〕.中华神经外科杂志,2004,20(1):59-62
[43]刘仰兵,赵永振.新生儿缺氧缺血性脑病的防治进展〔J〕.临床误诊误治,2005,18(11):778-779
[44]王杨,王琍琍,步仰高,等. 230例新生儿缺氧缺血性脑病10年回顾性调查及围产因素分析[J].中国全科医学,2004, 7(17): 1232-1233.
[45]刘映粼.高危妊娠监护治疗学[M].北京:北京医科大学·中国协和医科大学联合出版社, 1998. 99.
[46]陈长庚,胡旭红.新生儿窒息复苏及HIE发病机理新进展[J].四川卫生管理干部学院学报, 1999, 18(3): 95-96.
[47]孟娥,头针配合穴位注射早期治疗小儿缺氧缺血性脑病预防脑瘫.四川中医,2003,21(11):92一93
[48]景玉宏,王子仁.电针“百会”穴对大鼠记忆保持能力的影响及海马区ofs蛋白的表达.针刺研究,2004,6(2):107一110]
[49]何竟,黄长琼,张永玲等,针灸治疗小儿脑性瘫痪的临床文献质量评价和思考.中国针灸,2002,22(10):704一706
[50]吴美倩,张亦波.针刺对小儿脑瘫作用机制的初步探讨[J].江苏临床医学杂志,1998,6(7):464-467
[51]刘振寰,张鸿雁.头针为主治疗小儿脑瘫210例的临床研究[J].中国针灸,1999,19(1):651-653
[52]梁松,吴洋,高春霞,等.头针治疗小儿脑性瘫痪104例疗效分析[J].现代中西医结合杂志,2004,13(1):60-62
[53]厉华,水泉祥.小儿脑瘫[M].北京:北京医科大学/中国协和医科大学联合出版社,1996:119-149
[54]于海波,靳瑞.针刺对小儿脑瘫患者诱发肌电图的即时影响[J].中国针灸,1998,18(11):6-7
[55]施丙培,卜怀娣,季志英,等.针灸治疗对脑性瘫痪及精神发育迟缓患儿生长激素水平的影响[J].中国临床康复,2004,8(30):6627-6631
[56]施丙培,卜怀娣,赵瑞芳,等.针刺对小儿脑病骨钙素影响的观察[J].上海针灸杂志,2004,23(12):18-19
[57]丁春华,刘焕荣,张少丹,等.针灸治疗窒息脑瘫幼鼠的实验研究[J].河北医科大学学报,2000,21(3):155-156
[58]杜元灏,翟娜,李谈,等.针刺对脑梗塞模型大鼠脑传导纤维及尼氏体的影响.针灸临床杂志,1998,14:43-44.
[59]宛青,刘伟,吴爱群,等.电针对新生大鼠缺血缺氧后脑组织ChAT和BDNF表达的影响.解剖学研究,2003,25(4);295-297
[60]Mattson MP,Lovell MA,Furukawa K,et al.Neurotrophic factors attenuate glutamate-induced accumulation of peroxides,elevation of intracellular Ca2+ concentration, and neurotoxicity andincrease antioxidant enzyme activities in hippocampal neurons.JNeurochem,1995,65:1740-1751.
[61]Almli CR,levy TJ,Han BH,et al.BDNF protects against spatial memory deficits following neonatal hypoxia-ischemia. Exp Neurol,2000,166:99-114.
[62]Chen J,Simon R.Ischemic tolerance in the brain [J].Neurol,1997(2):306.
[63]Frim Dm,Short MP,Rosenbery WS,et al.Local protective effects of nerve growth factor secreting fibroblasts against excitotoxic le-sions in the rat striatum[J].J Neurosurg,1993,(2):267-273.
[64]Holtzm an DM,Sheldon RA,Jaffe W,et al.Nerve growth factor protects the neonatal brain against hypoxic- ischemic injury[J].Ann Neurol,1996,39(1):114-122.
[65]闫丙川,刘合玉,贾天明,等.针刺对缺氧缺血性脑损伤大鼠保护作用的实验研究.神经疾病与精神卫生,2005,5(1):33-35
[66]Nakatomi H, Kuriu T,Okabe S,et al.Regeneration of hippocampal pyramidal neurons after ischemic brain injury by recruitment of en-dogenousneural progenitors[J].Cell,2002,110(4):429-441.
[67]曾光伟,张伟宏,吴爱群,等。电针对缺血缺氧新生大鼠脑内神经前体细胞增殖的影响.郑州大学学报(医学版),2004,39(6):977-979
[68]Pham TM, W inblad B, Granholm AC, et al Environmental in-fluences on brain neurotrophins in rats. Pharmacol Biochem Be-hav, 2002, 73: 167-175.
[69]BRUEL-JUNGERMAN E, LAROCHE S, RAMPON C. New neurons in the dentate gyrus are involved in the expression of enhanced long-term memory following environmental enrichment[J]. Eur JNeurosi, 2005, 21(2): 513-521.
[70]SCHRIDDE U, VAN-LUIJTELAAR G. The role of the environmenton the development of spike-wave discharges in two strains of rats[J].PhysiolBehav, 2005, 84 (3): 379-386.
[71]PABAN V,JAFFARDM, CHAMBON C,etal T ime course ofbehav-ioral changes following basal forebrain cholinergic damage in rats:En-vironmental enrichment as a therapeutic intervention [J]. Neuro-science, 2005, 132(1): 13-32.
[72]鲁利群,赵聪敏,蒲昭霞.环境刺激对缺氧缺血性脑损伤大鼠海马巢蛋白表达及学习记忆的影响.第三军医大学学报,2006,28(8):804-806
[73]鲁利群,范建义,赵聪敏.环境刺激对缺氧缺血性脑损伤新生大鼠学习记忆及海马病理学的影响.实用儿科临床杂志,2007,22(2):103-105
[74]GobboOL, O′Mara SM. Impact of enriched-environment housing onbrain-derived neurotrophic factor and on cognitive performance after a transientglobal ischem ia[J].B ehav B rain Res,2004,152:231-241.
[75]Singer W. Development and plasticity of cortical processing architecture, Science, 1995,270:758-764.
[76]Abrahm IM, Kovcs KJ. Postnatalhandling alters the activation of stress-related neuronal circuitries.Eur JNeurosci,2000, 12:3003-3014.
[77]Yi Li,Ning Jiang, Cecylia Powers. Neurodamage and plasticity identified by MAP2, GAP43, CyclinDI immunoreactivity after fo-cal cerebral ischemia in rats. Stroke, 1998, 29: 1972-1980.
[78]李雪梅,蒲昭霞,周晓军。环境刺激对缺氧缺血性脑损伤新生大鼠海马微管相关蛋白-2表达的影响,中国新生儿科杂志,2008,23(1)31-34
[79]Stroemer RP,Kent TA,Hulsebosch CE.Neocortical neural sprouting,synaptogenesis,and behavioral recovery after neocortical infarction inrats.Stroke 1995;26:2135
[80]Schlaepfer WW.Neurofilament:structure,metabolism and implication in disease.J Neuropathol Exp Neurol 1987;46(3):117
[81]Briones TL,Woods J,Wadowska M,et al.Astrocytic changes in the hippocampus and functional recovery after cerebral ischemia are facilitated by rehabilitation training.Behavioural Brain Res 2006;171:17-25
[82]Matsumori Y,Hong SM,Fan Y,et al.Enriched environment and spatial learning enhance hippocampal neurogenesis and salvages ischemic penumbra after focal cerebral ischemia.Neurobiol Dis 2006;22(1):187-198
[83]Sonninen R,Virtanen T,Sivenius J,et al.Gene expression profiling in the hippocampus of rats subjected to focal cerebral ischemia and enriched environment housing.Restor Neurol Neurosci 2006;24(1):17-23
[84]Gama SM,Friedrich V,DegasperirR,et al.Human midsized neurofilament subunit induces motor neuron disease in transgenic mice.Exp Neurol 2004;184(l):408-419
[85]Pal J,Toth Z,Farkas O,et al.Selective induction of ultrastructural(neurofilament)compaction in axons by means of a new head-injury apparatus.Neurosci Methods 2006;153(2):283-289
[86]Letournel F,Bocquet A,Perrot R,et al.Neurofilament high molecular weight-green fluorescent protein fusion is normally expressed in neurons and transported in axons:a neuronal marker to investigate the biology of neurofilaments.Neuroscience 2006;137(1):103-111
[87]AL Chalabt A,Miller CC.Neurofilaments and neurological disease[J].Bioessays 2003;25(4):34
[88]Stanley EF.Presynaptic calcium channels and the depletion of synaptic cleft calcium.J Neurophysiol 2000;83(1):477-82
[89]于若谷,赵聪敏,王丽雁,等.丰富环境刺激对缺氧缺血性脑损伤大鼠脑超微结构改变及神经丝蛋白表达的影响.中国组织工程研究与临床康复,2007,11(13):2503-2506
[90]WHISHAW IQ, RODM R, AUER R N. Behavioral deficits revealed bymultiple tests in ratswith ischemic damage limited to halfof the CA1 sector of the hippocampus[J]. Brain ResBull 1994, 34(3): 283-289
[91]THIEL G. Synapsin I, synapsin II, and synaptophysin: marker pro-teins of synaptic vesicles [J]. Brain Pathol 1993, 3(1): 87-95
[92]蒲昭霞,赵聪敏,鲁利群,等.丰富环境对缺氧缺血性脑损伤大鼠海马突触超微结构及突触素表达的影响.第三军医大学学报,2006,28(8)816-818
[93]FernandazV, Adaro L, Sanhueza-TsutsumiM, et al Early-lifepoly sensorial stimulation and nutrition: topographic levels of sus-ceptibility in the rat visual cortex. BiolNeonate, 1997, 71: 265-276
[94]华兴邦.大鼠穴位图谱的研制.实验动物与动物实验,1991,(1):1-5
[95]Rosenberg AA,Muydaugh E.The effect of blood glucogse consentration on postasphyxia cerebal hemodynamics in newborn lambs.Pediatr Res ,1990;27:454
[96]John W.Calvert , Changman Zhou , et al.Zhang Hyperbaric oxygenation prevented brain injury induced by hypoxia-ischemia in a neonatal rat model[J].Brain Res,2002,951(l):l-8
[97]姚裕家,李炜如。地塞米松在新生猪缺氧缺血性脑损伤中的防治作用。中华儿科杂志,1994,32(4):201一202
[98]Vannucci RC,Vannucci SJ.A model of Perinatal hypoxic-ischemic brain damage[J].Ann NY Acad Sci,1997,835:234-249
[99]Riee J E,Vannucci RC,Brierley JB,etal.The influence of immaturity on hypoxic-ischemic brain damage in the rat[J].Ann Neurol , 1981 ,9:131-141
[100]Gilland E, Hagherg H.NMDA Receptor-dependent increase of perebral glucose utilization after hypoxia-ischemia in the immature rat.J CerebBlood Flow Metab.1996,16(5):1005-1013
[101]Vanucci RC,YagerJY,Vannucci SJ.Regional cerebral blood flow during hypoxia-ischemia in immature rats.Stroke,1988,19(2):245-250
[102]Romijn HJ, Homfan MA, Gramsbergen A.At what age is the developing cerebral cortex of the rat comparable to that of the full-term neubom human baby?EaHy Hum Dev,1991,26(1):61-67
[103]Hu BR,Liu CL,Ou yang Y,et al.Invol vement of caspase-3 in cell death after hypoxia-ischemia declines during brain maturation.J cered Blood Flow Metab,2000,20(9):1294-1300
[104]Muse ED, Jurevics H, Toews AD, et al.Parameters related to lipid metabolism as markers of myelination in mouse brain J Neurochem ,20()1;76(1):77-86
[105]Ginsberg MD,Busto R.Rodent models of cerebral isehemia.Storke,1989,20(12):1627-1642
[106]Vannuci RC,Vannucci SJ.Perinatal hypoxic-isehemic brain damage:evolution of an animal model.Dev Neurosci,2005,27(2-4):81-86
[107]汤健,张洪梅,吕静.小儿脑瘫头针治疗进展.南京中医药大学学报,2008,24(5):355-357
[108]孙忠人,霍立光,刘丽莉等.头皮针刺运动诱发电位的研究.中国中医药科技,1994,(16):14- 17
[109]唐强.头穴丛刺结合易化技术对脑卒中康复治疗作用的研究.博士后研究工作报告,2001,11-67
[110]早期干预降低早产儿脑性瘫痪发生率研究协作组.降低早产儿脑性瘫痪发生率的临床研究[J]。中华儿科杂志,2005,43(4):244一246
[111]Yager JY , Wright S , Armstrong EA,et al.Theinfluence of aging on recovery following isehemic brain damage[J].Behav Brain Res,2006,173(2):171-180
[112]Pham TM, W inb lad B, Granholm AC, et al Environmental in-fluences on brain neurotrophins in rats. Pharmacol Biochem Be-hav, 2002, 73: 167-175
[113]Markwoska AL, Spangler EL.Behavioral assessment of the senescence accelerated moues(SAM P8 and Rl),Physiol.Behav,1998,64:15一26
[114]Arteni NS , Salgueiro J.Neonatal cerebral hypoxia ischemia causes lateralized memory impairments in the adult rat.Brain Research,2003,973(2):171一178
[115]Abraham M, Kovacs KJ. Postnatal hand ling alters the activation of stress-related neuronal circuitries. Eur JN eurosci,2000, 12:3003-3014.
[116]Cristofan illiM, Thanos S, Brosius J, et al .Neuronal MAP2 mR-NA: species-dependent differential dendritic targeting compe-tence. JMol Biol, 2004, 341: 927-943
[117]Yi Li,Ning Jiang,Cecylia Powers.Neurodamage and plasticity identified by MAP2, GAP43, CyclinDI imm unoreactivity after fo-cal cerebral ischemia in rats. Stroke, 1998, 29: 1972-1980
[118]Daly C, Sugimori M, Moreira JE. Synaptophysin regulates clathrin independent endocytosis of synaptic vesicles[J].Proc Natl Acad,2000,97:6120-6125
[119]Leypoldt F, Flajolet M, Methner A. Neuronal differentiation of cultured human Ntera-2cl, DI cells leads to increased expression of synapsins[J]. Neurosci Lett,2002,324:37-40
[120]Ferreira A, Han HQ, Greegnard P, et al. Suppression of synapsin II inhibits the formation ad maintenance of synap- ses in hippocampal culture[J]. Proc Natl Acad Sci USA, 1995,92:9225-9229
[121]Mullany PM, Lynch MA. Evidence for a role for synato-physin in expression of long-term potentiation I rat dentate gyrus[J]. Neuroreport, 1998, 9:2489-2491
[122]蒲昭霞,赵聪敏,李亚伶等.脑发育不同阶段丰富环境刺激对大鼠海马突触素表达的影响.中国儿童保健杂志,2007,15(6):632-634
[123]宋文秀,曹云涛,刘华庆.新生鼠脑缺血再灌注后海马CA1区突触素的表达及意义[J].第四军医大学学报,2004,25(16):1467-1469
[124]WHISHAW IQ, RODM R, AUER R N. Behavioral deficits revealed by multipletests in ratswith ischemic damage limited to halfof theCA1 sector of the hippocampus [J]. Brain ResBull, 1994, 34(3): 283-289
[125]THIEL G. Synapsin I, synapsin II, and synaptophysin: marker pro-teins of synaptic vesicles [J]. Brain Pathol, 1993, 3(1): 87-95
[126]田春海,周丛乐,苗鸿才.新生大鼠缺氧缺血后远期海马CA1区神经元和突触的变化及干预的效果[J].中华围产医学杂志,1999, 2(1): 33-35