单核细胞趋化蛋白-1对大鼠试验性视网膜脱离过程中光感受器细胞凋亡的影响
|
摘要
目的:1.探讨MCP-1在Brown Norway大鼠试验性视网膜脱离中的表达,及其与视网膜脱离过程中光感受器细胞凋亡的相关性。2.探讨干预脱离的视网膜MCP-1的表达,光感受器细胞凋亡的变化。
方法:(1)取37只Brown Norway大鼠右眼建立视网膜脱离模型,左眼自身对照。排除因死亡,眼内炎和视网膜出血等不符合视网膜脱离模型标准的大鼠7只。剩余30只大鼠按预处理时间点(1小时,6小时,12小时,24小时和72小时)随机分为五组,每组6只。预定时间点处死大鼠,取出眼球。免疫组化技术检测实验眼与对照眼视网膜外核层MCP-1的表达;TUNEL原位末端标记法检测实验眼与对照眼外核层凋亡细胞阳性表达细胞数。统计软件分析MCP-1与TUNEL表达的相关性。(2)9只Brown Norway大鼠双眼建立视网膜脱离模型后,沿原穿刺口右眼视网膜下注射抗大鼠MCP-1 IgG抗体,左眼注射等容积的PBS。排除因死亡,眼内炎和视网膜出血等不符合视网膜脱离模型标准的大鼠3只。剩余6只大鼠模型建立后72小时处死,剜出眼球,制作石蜡切片,应用TUNEL原位末端标记法计数外核层阳性细胞。统计学方法比较72小时视网膜脱离组,MCP-1干预组和假干预组的外核层凋亡细胞的表达。
结果:(1)免疫组化:正常视网膜MCP-1很少表达,点状分布于内核层,外核层和神经节细胞层,每个高倍镜下外核层阳性表达细胞数平均为3.64±1.02。试验性视网膜脱离模型建立1小时、6小时、12小时、24小时和72小时后MCP-1表达主要分布于外核层,内核层与神经节细胞层较少表达,外核层高倍镜下MCP-1阳性表达细胞数分别为10.50±2.82、11.13±3.31、25.23±5.56、37.23±7.15和205.83±15.23,与对照组均有统计学差异。视网膜脱离组之间相互比较,除视网膜脱离1小时和6小时无统计学差异外,其余各组均有显著性差异(P＜0.05)。(2)TUNEL:正常视网膜外核层TUNEL阳性细胞数为2.16±1.33/mm~2,试验性视网膜脱离模型建立1小时、6小时、12小时、24小时和72小时外核层TUNEL阳性表达细胞数分别为15.30±3.21/mm~2、25.11±5.31/mm~2、41.32±7.56/mm~2、80.23±10.15/mm~2和425.83±25.23/mm~2。(3)各组大鼠实验眼视网膜ONL内MCP-1及TUNEL阳性细胞均于RD后1h开始升高,随时间延长表达增加,72小时达到高峰,二者的表达呈显著正相关(r=0.905)。(4)视网膜脱离后72小时MCP-1干预组和PB S假干预组外核层TUNEL阳性表达细胞数为160.24±10.56/mm~2和430.15±30.42/mm~2。MCP-1干预组与视网膜脱离组统计学上具有显著性差异(p＜0.05),而PBS假干预组与视网膜脱离组统计学无显著性差异(p＞0.05)。
结论:(1)MCP-1在大鼠试验性视网膜脱离模型建立后1小时即明显表达,随时间延长表达增加,72小时达到高峰。(2)视网膜脱离各时相的MCP-1与TUNEL阳性表达细胞数具有明显正相关。(3)干预脱离的视网膜MCP-1的表达可减少光感受器细胞的凋亡。
Objective:(1)To explore Monocyte chemoattractant protein-1 expresses in rat experimental retinal detachment at different times;the relationship between MCP-1 and photoreceptor apoptosis in retinal detachment.(2)To explore blocking the expression of MCP-1 in retinal detachment can diminish photoreceptor apoptosis.
Methods:46 Brown Norway rats were involved in this study.(1)37 rats were builded the retinal detachment model in the right eye,and the left eye as control.Except 7 rats which were not fit to the standard model(include death, endophthalmitis and retinal hemorrhage).The left 30 rats devided into 5 groups according force-managed times(1h、6h、12h、24h、72h).Then rats were killed and the eyes were enclutered.The expression of MCP-1 in retinal detachment eyes were assessed by immunohistochemical method and cell death were quantified by TUNEL assay.Analysis the relatinship between MCP-1 and TUNEL in detached retinal by statistical software.(2)9 rats were builded the retinal detachment model both of the eyes.We injected rat anti-MCP-1 IgG subretinally during RD induction in the right eyes and PBS in the left eyes. Except 3 rats which were not fit to the standard model(include death,endophthalmitis and retinal hemorrhage).Cell death were quantified by TUNEL assay 72h after RD,since at that time the number of TUNEL-positive cells in the ONL is at the peak.
Resuits(1)Immunohistochemical analysis:In normal retinal tissue,the number of MCP-1 positive cells was 3.64±1.02 under microscopy visual field.MCP-1 positive cells was 10.50±2.82、11.13±3.31、25.23±5.56、37.23±7.15、205.83±15.23 in detached retinal at 1h、6h、12h、24h、72h under microscopy visual field.Compare the expression of MCP-1 at different times of RD,every two groups had statistical variation,except the 1h and 6h group.(2)TUNEL assey:In normal retinal tissue,the number of TUNEL positive cells in ONL was 2.16±1.33/mm~2.TUNEL positive cells in ONL was 15.30±3.21/mm~2;25.11±5.31/mm~2;41.32±7.56/mm~2; 80.23±10.15/mm~2;425.83±25.23/mm~2 in detached retinal at 1h、6h、12h、24h、72h.(3)MCP-1 and TUNEL positive cell began to express remarkably after 1h of retinal detachment,then increased follow time,got the peak at 72h.The relationship between MCP-1 and TUNEL positive cell is positive correlation(r=0.905).(4)TUNEL positive cells in MCP-1 blocking group and PBS group after 72h of retinal detachment were 160.24±10.56/mm~2 and 430.15±30.42/mm~2 in ONL.There was statistical variation between MCP-1 blocking group and RD group(p＜0.05),no statistical variance between RD group and PBS group(p＞0.05).
Conclution:(1)MCP-1 began to express after 1h of rat retinal detachment,then increased follow time,got the peak at 72h.(2)There is positive correlation between MCP-1 and TUNEL positive cell.(3)Blocking the expression of MCP-1 in detached retinal can diminish photoreceptor apoptosis.
方法:(1)取37只Brown Norway大鼠右眼建立视网膜脱离模型,左眼自身对照。排除因死亡,眼内炎和视网膜出血等不符合视网膜脱离模型标准的大鼠7只。剩余30只大鼠按预处理时间点(1小时,6小时,12小时,24小时和72小时)随机分为五组,每组6只。预定时间点处死大鼠,取出眼球。免疫组化技术检测实验眼与对照眼视网膜外核层MCP-1的表达;TUNEL原位末端标记法检测实验眼与对照眼外核层凋亡细胞阳性表达细胞数。统计软件分析MCP-1与TUNEL表达的相关性。(2)9只Brown Norway大鼠双眼建立视网膜脱离模型后,沿原穿刺口右眼视网膜下注射抗大鼠MCP-1 IgG抗体,左眼注射等容积的PBS。排除因死亡,眼内炎和视网膜出血等不符合视网膜脱离模型标准的大鼠3只。剩余6只大鼠模型建立后72小时处死,剜出眼球,制作石蜡切片,应用TUNEL原位末端标记法计数外核层阳性细胞。统计学方法比较72小时视网膜脱离组,MCP-1干预组和假干预组的外核层凋亡细胞的表达。
结果:(1)免疫组化:正常视网膜MCP-1很少表达,点状分布于内核层,外核层和神经节细胞层,每个高倍镜下外核层阳性表达细胞数平均为3.64±1.02。试验性视网膜脱离模型建立1小时、6小时、12小时、24小时和72小时后MCP-1表达主要分布于外核层,内核层与神经节细胞层较少表达,外核层高倍镜下MCP-1阳性表达细胞数分别为10.50±2.82、11.13±3.31、25.23±5.56、37.23±7.15和205.83±15.23,与对照组均有统计学差异。视网膜脱离组之间相互比较,除视网膜脱离1小时和6小时无统计学差异外,其余各组均有显著性差异(P＜0.05)。(2)TUNEL:正常视网膜外核层TUNEL阳性细胞数为2.16±1.33/mm~2,试验性视网膜脱离模型建立1小时、6小时、12小时、24小时和72小时外核层TUNEL阳性表达细胞数分别为15.30±3.21/mm~2、25.11±5.31/mm~2、41.32±7.56/mm~2、80.23±10.15/mm~2和425.83±25.23/mm~2。(3)各组大鼠实验眼视网膜ONL内MCP-1及TUNEL阳性细胞均于RD后1h开始升高,随时间延长表达增加,72小时达到高峰,二者的表达呈显著正相关(r=0.905)。(4)视网膜脱离后72小时MCP-1干预组和PB S假干预组外核层TUNEL阳性表达细胞数为160.24±10.56/mm~2和430.15±30.42/mm~2。MCP-1干预组与视网膜脱离组统计学上具有显著性差异(p＜0.05),而PBS假干预组与视网膜脱离组统计学无显著性差异(p＞0.05)。
结论:(1)MCP-1在大鼠试验性视网膜脱离模型建立后1小时即明显表达,随时间延长表达增加,72小时达到高峰。(2)视网膜脱离各时相的MCP-1与TUNEL阳性表达细胞数具有明显正相关。(3)干预脱离的视网膜MCP-1的表达可减少光感受器细胞的凋亡。
Objective:(1)To explore Monocyte chemoattractant protein-1 expresses in rat experimental retinal detachment at different times;the relationship between MCP-1 and photoreceptor apoptosis in retinal detachment.(2)To explore blocking the expression of MCP-1 in retinal detachment can diminish photoreceptor apoptosis.
Methods:46 Brown Norway rats were involved in this study.(1)37 rats were builded the retinal detachment model in the right eye,and the left eye as control.Except 7 rats which were not fit to the standard model(include death, endophthalmitis and retinal hemorrhage).The left 30 rats devided into 5 groups according force-managed times(1h、6h、12h、24h、72h).Then rats were killed and the eyes were enclutered.The expression of MCP-1 in retinal detachment eyes were assessed by immunohistochemical method and cell death were quantified by TUNEL assay.Analysis the relatinship between MCP-1 and TUNEL in detached retinal by statistical software.(2)9 rats were builded the retinal detachment model both of the eyes.We injected rat anti-MCP-1 IgG subretinally during RD induction in the right eyes and PBS in the left eyes. Except 3 rats which were not fit to the standard model(include death,endophthalmitis and retinal hemorrhage).Cell death were quantified by TUNEL assay 72h after RD,since at that time the number of TUNEL-positive cells in the ONL is at the peak.
Resuits(1)Immunohistochemical analysis:In normal retinal tissue,the number of MCP-1 positive cells was 3.64±1.02 under microscopy visual field.MCP-1 positive cells was 10.50±2.82、11.13±3.31、25.23±5.56、37.23±7.15、205.83±15.23 in detached retinal at 1h、6h、12h、24h、72h under microscopy visual field.Compare the expression of MCP-1 at different times of RD,every two groups had statistical variation,except the 1h and 6h group.(2)TUNEL assey:In normal retinal tissue,the number of TUNEL positive cells in ONL was 2.16±1.33/mm~2.TUNEL positive cells in ONL was 15.30±3.21/mm~2;25.11±5.31/mm~2;41.32±7.56/mm~2; 80.23±10.15/mm~2;425.83±25.23/mm~2 in detached retinal at 1h、6h、12h、24h、72h.(3)MCP-1 and TUNEL positive cell began to express remarkably after 1h of retinal detachment,then increased follow time,got the peak at 72h.The relationship between MCP-1 and TUNEL positive cell is positive correlation(r=0.905).(4)TUNEL positive cells in MCP-1 blocking group and PBS group after 72h of retinal detachment were 160.24±10.56/mm~2 and 430.15±30.42/mm~2 in ONL.There was statistical variation between MCP-1 blocking group and RD group(p＜0.05),no statistical variance between RD group and PBS group(p＞0.05).
Conclution:(1)MCP-1 began to express after 1h of rat retinal detachment,then increased follow time,got the peak at 72h.(2)There is positive correlation between MCP-1 and TUNEL positive cell.(3)Blocking the expression of MCP-1 in detached retinal can diminish photoreceptor apoptosis.
引文
1 贾玲,余涵,李超,等.视网膜电图对视网膜脱离术后效果的临床分析[J].眼科新进展Yan ke Xinjin zhan 2002,22(5):351-352
2 孙晓东,张皙,余彰,等.实验性视网膜脱离复位后的视网膜超微结构观察[J].眼科研究 2001,19(5):326-329
3 Zacks DN,Hanninen V,Pantcheva M,et al.Caspase activation in an experimental model of retinal detachment.Invest Ophthalmol Vis Sci 2003,44:1262-1267
4 Hisatomi T,Sakamoto T,Goto Y,et al.Critical role of photoreceptor apoptosis in functional damage after retinal detachment.Curr Eye Res 2002,24:161-172
5 Hisatomi T,Sakamoto T,Murata T,et al.Relocalization of apoptosis-inducing factor in photoreceptor apoptosis induced by retinal detachment in vivo.Am J Pathol,2001,158:1271-1278
6 Toru Nakazawa,Akihisa Matsubara,Kousuke Noda,et al.Characterization of cytokine responses to retinal detachment in rats.Molecular Vision 2006,12:867-78
7 Reape TJ,Groot PH.Chemokines and atherosclerosis.Atherosclerosis,1999,147:213-225
8 Huffnagle GB,Traynor TR,McDonald RA,et al.Leukocyte recruitment during pulmonary Cryptococcus neoformans infection.Immunopharmacology,2000,48:231-236
9 Ishida S,Yamashiro K,Usui T,et al.Leukocytes mediate retinal vascular remodeling during development and vaso-obliteration in disease.Nat Med,2003,9:781-788
10 Nakao S,Kuwano T,Tsutsumi-Miyahara C,et al.Infiltration of COX-2-expressing macrophages is a prerequisite for IL-1 beta-induced neovascularization and tumor growth(2005)J Clin Invest 115:2979-2991
11 Fenoglio C,Galimberti D,Lovati C,et al.MCP-1 in Alzheimer's disease patients:A-2518G polymorphism and serum levels.(2004)Neurobiol Aging 25:1169-1173
12 Mahad DJ,Ransohoff RM.The role of MCP-1(CCL2)and CCR2 in multiple sclerosis and experimental autoimmune encephalomyelitis(EAE).(2003)Semin Immunol 15:23-32
13 Nishimura M,Kuno S.Mizuta I.Influence of monocyte chemoattractant protein 1gene polymorphism on age at onset of sporadic Parkinson's disease(2003)Movement Disorders 18:953-955
14 Chen L,Wu W,Dentchev T,et al.Light damage induced changes in mouse retinal gene expression(2004)Exp Eye Res 79:239-247
15 Crane IJ,McKillop-Smith S,Wallace CA,et al.Expression of the chemokines MIP-lalpha,MCP-1.and RANTES in experimental autoimmune uveitis(2001)Invest Ophthalmol Vis Sci 42:1547-1552
16 Meleth AD,Agron E,Chan CC,et al.Serum inflammatory markers in diabetic retinopathy(2005)Invest Ophthalmol Vis Sci 46:4295-4301
17 Zeng HY,Zhu XA.Zhang C.et al.Identification of sequential events and factors associated with microglial activation,migration,and cytotoxicity in retinal degeneration in rd mice.(2005)Invest Ophthalmol Vis Sci 46:2992-2999
18 Jo N,Wu GS,Rao NA.Upregulation of chemokine expression in the retinal vasculature in ischemia-reperfusion injury.(2003)Invest Ophthalmol Vis Sci 44:4054-4060
19 Banas B,Luckow B.MollerM,et al.Chemokine and chemokine receptor expression in novel human mesangial cell line[J].J Am Soc Nephrol.1999,10(11):2314-2322
20 Hisatomi T,Sakamoto T.Goto Y,et al.Critical role of photoreceptor apoptosis in functional damage after retinal detachment.Curr Eye Res 2002;24:161-72
21 刘铁成.实验性视网膜脱离与复位中“接触抑制”现象观察.眼科新进展Yan ke Xin jin zhan.2005,5:134-140
22 陈有信,金曼林.何世坤,等.肝细胞生长因子诱导实验性视网膜脱离的病理机制研究.中华眼底病杂志,2007,03:15-19
23 王卉芳,张妍春.朱赛林,等.实验性网脱复位后术脱离区域视网膜组织形态学观察.陕西医学杂志,2006,08:134-140,24
24 王建洲.惠延年,王雨生,等.内皮素在实验性视网膜脱离中的表达.中华眼底病杂志,2006,05:120-125
25 Matsumoto Y.TakahashiM.Ogata M.Relationship between glycoxidation and cytokine in the vitreous of eye with diabetic retinopathy [J]. Jpn J Ophthalmol 2002;46:406-412
26 Libby P, Sukhova G, Lee RT, et al. Cytokines regulate vascular functions related to stability of the atherosclerotic plaque [ J ]. J Cardiovasc Pharmacol 1995; 25:S9 -S12
27 Toru Nakazawa, Akihisa Matsubara, Kousuke Noda, et al. Characterization of cytokine responses to retinal detachment in rats. Molecular Vision 2006; 12:867-78
28 Hisatomi T, Sakamoto T, Sonoda KH,et al. Clearance of apoptotic photoreceptors:elimination of apoptotic debris into the subretinal space and macrophagemediated phagocytosis via phosphatidylserine receptor and integrin alphavbeta3. Am J Pathol 2003; 162:1869-79
29 Jacobson MD, Weil M. Raf MC. Programmed cell death in animal development.Cell,1997,88:347-349
30 Yang J ,Liu X ,Bhalla K,et al .Prevention of apoptosis by Bcl-2:release of Cytochrome C from mitochondrial control of apoptosis.Science. 1998,281: 2027-31
31 Nagata S .Apoptosis by death factor.Cell.1997,88:355-357
32 Dechant G, Barde Y-A. Signalling through the neurotropin receptorp75NTR. Curr Opi Neurobiol,1997,7:413-417
33 Rosner M, Lam T T, Fu J,et al. Methylprednisolone ameliorates retinal photic injury in rats.Arch Ophthalmol (1992) Arch. Ophthalmol. 110,857-861
34 Donovan M, Carmody R J, Cotter T G. Light-induced photoreceptor apoptosis in vivo requires neuronal nitric-oxide synthase and guanylate cyclase activity and is caspase-3-independent (2001) J. Biol. Chem. 276,23000-23008
35 Yamazaki H, Ohguro H. Maeda T,et al. (2002) Investig. Ophthalmol.Vis. Sci. 43,919-926
36 Banisadr G, Queraud-Lesaux F. Boutterin MC, Pelaprat D, Zalc B, Rostene W,Haour F, Parsadaniantz SM. Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain. J Neurochem 2002; 81:257-69
37 Crawford M J. Krishnamoorthy R R. Rudick V L,et al. (2001) Biochem.Biophys.Res. Commun. 281, 1304-1312
38 Toru Nakazawa,Toshio Hisatomi.Chifuyu Nakazawa.et al.Monocyte chemoattractant protein 1 mediates retinal detachment-induced photoreceptor apoptosis.Proc Natl Acad Sci U S A.2007 Feb 13;104(7):2425-30
39 Hisatomi T. Sakamoto T, Sonoda Kh,et al.Clearance of apoptotic photoreceptors:elimination of apoptotic debris into the subretinal space and macrophage mediated phagocytosis via phosphatidylserine receptor and integrin alphavbeta3. Am J Pathol 2003; 162:1869-79
40 Dzenko KA, Andjelkovic AV, Kuziel WA. et al. The chemokine receptor CCR2 mediates the binding and internalization of monocyte chemoattractant protein-1 along brain microvessels. J Neurosci 2001; 21:9214-23
41 van Gassen KL, Netzeband JG, de Graan PN.et al. The chemokine CCL2 modulates Ca2+ dynamics and electrophysiological properties of cultured cerebellar Purkinje neurons. Eur J Neurosci 2005; 21:2949-57
42 Sharma AK, Rohrer B. Calcium-induced calpain mediates apoptosis via caspase-3 in a mouse photoreceptor cell line. J Biol Chem 2004; 279:35564-72
21
[1]陈百华,姜德咏.周细胞与糖尿病视网膜病变[J]眼科新进展,2002;22(2):133-134
[2]Rovin BH,Yoshimura T,Tan L.Cytokine-induced production of monocyte chemoattractant protein-1 by cultured human mesangial cell[J].J Immunol.1992;148(7):2148-53
[3]王国平,邓仲端.MCP-1及其基因的结构和功能[J]国外医学生理、病理科学和临床分册,1996;16(2):67-69
[4]Yoshimura T,yuhki N,moore SK,et al.Human monocyte chemoattractant protein-l(MCP-1)full length cDNA cloning,expression in mitrgen-stimulted blood mononuclear leukocytes,and sequence similarity to mouse competence gene JE[J]Feb slett,1989;244(2):487-493
[5]Yoshimura T,Robinson EA,Tanaks S,et al.Purification and amino acid analysis of two glioma-devided monocyte chemoattractants[J].J Exp Med,1989,169(4):1449-59
[6]Shyy YJ,Li YS,Kolattukudy PE.Structure of human monocyte chemtactic protein gene and its regulation by TPA[J].Bjochem Biophy Res Common,1990,169(2):346-51
[7]Sica A,Wang JM,Colotta F,et al.Monocyte chemotactic and activating factor gene expression induced in endothelial cells by IL-1 and tumer necrosis factor[J].J Immunol,1990,144(8):3034-8
[8]曾钧,宋烈杰.单核细胞趋化蛋白-1与炎性疾病的研究现状.第一军医大学学报 2001;21(12):84-86
[9]Rollins BJ.et al.Blood.1991:78(4):1112-1116
[10]Shyy YJ.et al.J Imunol.1993:92:1745-1751
Ill]Ward SG,West wick J.Chemokines understanding their role in T2 lymphocyte biology[J].Biochem J,1998,333:457-470
[12]Neote K.Cell,1993;72:415-425
[13]Murphy PM.Cytokine Growth Factor.Rev,1996;7:47-64
[14]刘杰,孙晗笑.趋化因子及其受体转导途径与肿瘤的生长.Chemistry of life 2003;23(1):38-40
[15]赵志勇,周予兰,王伟.等.肿瘤坏死因子对糖尿病视网膜病变的珍断价值[J]中国误诊学杂志 2007,7(1)65:66
[16]宋鄂.王秋利,杨威,等.碱性成纤维细胞生长因子在糖尿病大鼠视网膜血管中的表达[J]眼科研究,2004,22,(3)290:293
[17]郑建秋,郭庚,腾岩.糖尿病大鼠视网膜早期病变组织中生长因子作用的实验研究[J].中华实用眼科杂志 2001,7,(19)494:497
[18]Mrudula T,Suryanarayana P,Srinivas PN,et al.Effect of curcumin on hyperglycemia-induced vascular endothelial growth factor expression in streptozotocin-induced diabetic rat retina.[J]Biochem Biophys Res Commun,2007,Sep 21:361(2):528-532
[19]Chung HS,Harris A,Halter PJ,et al.Regional differences in retinal vascular reactivity[J].Invest Ophthalmol Vis Sci 1999;40:2448-2453
[20]Banas B,Luckow B,Moller M,et al.Chemokine and chemokine receptor expression in novel human mesangial cell line[J].J Am Soc Nephrol 1999;10(11):2314-2322
[21]Ward SG,Westwick J.Chemokines understanding their role in T2 lymphocyte biology[J].Biochem J 1998;333:457-470
[22]Matsumoto Y.TakahashiM,Ogata M.Relationship between glycoxidation and cytokine in the vitreous of eye with diabetic retinopathy[J].Jpn J Ophthalmol 2002:46:406-412
[23]Mitamura Y,Takeuchi S,Matsuda A,et al.Monocyte chemotactic protein-1 in the vitreous of patients with proliferative diabetic retinopathy[J]Ophthalmologica 2001;215:415-418
[24]Abuel Asrar AM,Van Damme J,Put W,et al.Monocyte chemotactic protein-1 in proliferative vitreoretinal disorders[J].Am J Ophthalmol 1997:12:599-606
[25]Marumo T.Schini Kerth V B,Busse R.Vascular endothelial growth factor activates nuclear factor kappaβ and induces monocyte chemoattractant protein-1in bovine retinal endothelial cells[J]Diabetes 1999;48:1131-1137
[26] Sozzani S, Locat M, Zhou D, et al. Recep tors, signal transduction, and spectrum of action of monocyte chemotactic protein-1 and related chemokines[J]. J Leukocyte Biol 1995; 57:788 - 794
[27] Libby P, Sukhova G, Lee RT, et al. Cytokines regulate vascular functions related to stability of the atherosclerotic plaque [J]. J Cardiovasc Pharmacol 1995; 25:S9-S12
[28] Viberti GC, Earle K. Predisposition to essential hypertension and the development of diabetic nephropathy. J Am Soc Nephrol. 1992;3:S27-S33
[29] Klein BE, Moss SE, Klein R, et al. The Wisconsin epidemiologic study of diabetic retinopathy. J Am Soc Nephrol. 1997;4:S28-S35
[30] Antonia M, Oussen, Toshinori Murata, et al. Leukocyte-Mediated Endothelial Cell Injury And Death in the Diabetic Retina. Am J Pathol, 2001,158:147-152
[31] Cohen MP . Clements RS . Cohen JA ,et al.Relationship of serum cholesterol to retinopethy and hard exudates[J]. Ophthalmology, 1991, 98(8)11-61
[32] Kato S ,Luyckx V A , Ots M,et al.monocyte chemoattractant protein-1 in cytokine treated human vascular endothelial cells [J]. FEB S Lett 1999; 454(1-2):27-30
[33] Kato S ,Luyckx V A , Ots M.et al. Reninangiotensin blockade lowers MCP-1 expression in diabetic rats[J].Kidney Int 1999;56 :1037-1048
[34] Cohen MP , Clements RS . Cohen JA ,et al. Prevention of d in the diabetic db/ db mouse[J ] .Diabetologia996;39:270-274
[35] Hagedorn M, Zilberberg L . Lozano R M. et al. A short peptide domain of platelet factor 4 blocks angiogenic key events induced by FGF22[J].FASEB J 2001;15:550-552
2 孙晓东,张皙,余彰,等.实验性视网膜脱离复位后的视网膜超微结构观察[J].眼科研究 2001,19(5):326-329
3 Zacks DN,Hanninen V,Pantcheva M,et al.Caspase activation in an experimental model of retinal detachment.Invest Ophthalmol Vis Sci 2003,44:1262-1267
4 Hisatomi T,Sakamoto T,Goto Y,et al.Critical role of photoreceptor apoptosis in functional damage after retinal detachment.Curr Eye Res 2002,24:161-172
5 Hisatomi T,Sakamoto T,Murata T,et al.Relocalization of apoptosis-inducing factor in photoreceptor apoptosis induced by retinal detachment in vivo.Am J Pathol,2001,158:1271-1278
6 Toru Nakazawa,Akihisa Matsubara,Kousuke Noda,et al.Characterization of cytokine responses to retinal detachment in rats.Molecular Vision 2006,12:867-78
7 Reape TJ,Groot PH.Chemokines and atherosclerosis.Atherosclerosis,1999,147:213-225
8 Huffnagle GB,Traynor TR,McDonald RA,et al.Leukocyte recruitment during pulmonary Cryptococcus neoformans infection.Immunopharmacology,2000,48:231-236
9 Ishida S,Yamashiro K,Usui T,et al.Leukocytes mediate retinal vascular remodeling during development and vaso-obliteration in disease.Nat Med,2003,9:781-788
10 Nakao S,Kuwano T,Tsutsumi-Miyahara C,et al.Infiltration of COX-2-expressing macrophages is a prerequisite for IL-1 beta-induced neovascularization and tumor growth(2005)J Clin Invest 115:2979-2991
11 Fenoglio C,Galimberti D,Lovati C,et al.MCP-1 in Alzheimer's disease patients:A-2518G polymorphism and serum levels.(2004)Neurobiol Aging 25:1169-1173
12 Mahad DJ,Ransohoff RM.The role of MCP-1(CCL2)and CCR2 in multiple sclerosis and experimental autoimmune encephalomyelitis(EAE).(2003)Semin Immunol 15:23-32
13 Nishimura M,Kuno S.Mizuta I.Influence of monocyte chemoattractant protein 1gene polymorphism on age at onset of sporadic Parkinson's disease(2003)Movement Disorders 18:953-955
14 Chen L,Wu W,Dentchev T,et al.Light damage induced changes in mouse retinal gene expression(2004)Exp Eye Res 79:239-247
15 Crane IJ,McKillop-Smith S,Wallace CA,et al.Expression of the chemokines MIP-lalpha,MCP-1.and RANTES in experimental autoimmune uveitis(2001)Invest Ophthalmol Vis Sci 42:1547-1552
16 Meleth AD,Agron E,Chan CC,et al.Serum inflammatory markers in diabetic retinopathy(2005)Invest Ophthalmol Vis Sci 46:4295-4301
17 Zeng HY,Zhu XA.Zhang C.et al.Identification of sequential events and factors associated with microglial activation,migration,and cytotoxicity in retinal degeneration in rd mice.(2005)Invest Ophthalmol Vis Sci 46:2992-2999
18 Jo N,Wu GS,Rao NA.Upregulation of chemokine expression in the retinal vasculature in ischemia-reperfusion injury.(2003)Invest Ophthalmol Vis Sci 44:4054-4060
19 Banas B,Luckow B.MollerM,et al.Chemokine and chemokine receptor expression in novel human mesangial cell line[J].J Am Soc Nephrol.1999,10(11):2314-2322
20 Hisatomi T,Sakamoto T.Goto Y,et al.Critical role of photoreceptor apoptosis in functional damage after retinal detachment.Curr Eye Res 2002;24:161-72
21 刘铁成.实验性视网膜脱离与复位中“接触抑制”现象观察.眼科新进展Yan ke Xin jin zhan.2005,5:134-140
22 陈有信,金曼林.何世坤,等.肝细胞生长因子诱导实验性视网膜脱离的病理机制研究.中华眼底病杂志,2007,03:15-19
23 王卉芳,张妍春.朱赛林,等.实验性网脱复位后术脱离区域视网膜组织形态学观察.陕西医学杂志,2006,08:134-140,24
24 王建洲.惠延年,王雨生,等.内皮素在实验性视网膜脱离中的表达.中华眼底病杂志,2006,05:120-125
25 Matsumoto Y.TakahashiM.Ogata M.Relationship between glycoxidation and cytokine in the vitreous of eye with diabetic retinopathy [J]. Jpn J Ophthalmol 2002;46:406-412
26 Libby P, Sukhova G, Lee RT, et al. Cytokines regulate vascular functions related to stability of the atherosclerotic plaque [ J ]. J Cardiovasc Pharmacol 1995; 25:S9 -S12
27 Toru Nakazawa, Akihisa Matsubara, Kousuke Noda, et al. Characterization of cytokine responses to retinal detachment in rats. Molecular Vision 2006; 12:867-78
28 Hisatomi T, Sakamoto T, Sonoda KH,et al. Clearance of apoptotic photoreceptors:elimination of apoptotic debris into the subretinal space and macrophagemediated phagocytosis via phosphatidylserine receptor and integrin alphavbeta3. Am J Pathol 2003; 162:1869-79
29 Jacobson MD, Weil M. Raf MC. Programmed cell death in animal development.Cell,1997,88:347-349
30 Yang J ,Liu X ,Bhalla K,et al .Prevention of apoptosis by Bcl-2:release of Cytochrome C from mitochondrial control of apoptosis.Science. 1998,281: 2027-31
31 Nagata S .Apoptosis by death factor.Cell.1997,88:355-357
32 Dechant G, Barde Y-A. Signalling through the neurotropin receptorp75NTR. Curr Opi Neurobiol,1997,7:413-417
33 Rosner M, Lam T T, Fu J,et al. Methylprednisolone ameliorates retinal photic injury in rats.Arch Ophthalmol (1992) Arch. Ophthalmol. 110,857-861
34 Donovan M, Carmody R J, Cotter T G. Light-induced photoreceptor apoptosis in vivo requires neuronal nitric-oxide synthase and guanylate cyclase activity and is caspase-3-independent (2001) J. Biol. Chem. 276,23000-23008
35 Yamazaki H, Ohguro H. Maeda T,et al. (2002) Investig. Ophthalmol.Vis. Sci. 43,919-926
36 Banisadr G, Queraud-Lesaux F. Boutterin MC, Pelaprat D, Zalc B, Rostene W,Haour F, Parsadaniantz SM. Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain. J Neurochem 2002; 81:257-69
37 Crawford M J. Krishnamoorthy R R. Rudick V L,et al. (2001) Biochem.Biophys.Res. Commun. 281, 1304-1312
38 Toru Nakazawa,Toshio Hisatomi.Chifuyu Nakazawa.et al.Monocyte chemoattractant protein 1 mediates retinal detachment-induced photoreceptor apoptosis.Proc Natl Acad Sci U S A.2007 Feb 13;104(7):2425-30
39 Hisatomi T. Sakamoto T, Sonoda Kh,et al.Clearance of apoptotic photoreceptors:elimination of apoptotic debris into the subretinal space and macrophage mediated phagocytosis via phosphatidylserine receptor and integrin alphavbeta3. Am J Pathol 2003; 162:1869-79
40 Dzenko KA, Andjelkovic AV, Kuziel WA. et al. The chemokine receptor CCR2 mediates the binding and internalization of monocyte chemoattractant protein-1 along brain microvessels. J Neurosci 2001; 21:9214-23
41 van Gassen KL, Netzeband JG, de Graan PN.et al. The chemokine CCL2 modulates Ca2+ dynamics and electrophysiological properties of cultured cerebellar Purkinje neurons. Eur J Neurosci 2005; 21:2949-57
42 Sharma AK, Rohrer B. Calcium-induced calpain mediates apoptosis via caspase-3 in a mouse photoreceptor cell line. J Biol Chem 2004; 279:35564-72
21
[1]陈百华,姜德咏.周细胞与糖尿病视网膜病变[J]眼科新进展,2002;22(2):133-134
[2]Rovin BH,Yoshimura T,Tan L.Cytokine-induced production of monocyte chemoattractant protein-1 by cultured human mesangial cell[J].J Immunol.1992;148(7):2148-53
[3]王国平,邓仲端.MCP-1及其基因的结构和功能[J]国外医学生理、病理科学和临床分册,1996;16(2):67-69
[4]Yoshimura T,yuhki N,moore SK,et al.Human monocyte chemoattractant protein-l(MCP-1)full length cDNA cloning,expression in mitrgen-stimulted blood mononuclear leukocytes,and sequence similarity to mouse competence gene JE[J]Feb slett,1989;244(2):487-493
[5]Yoshimura T,Robinson EA,Tanaks S,et al.Purification and amino acid analysis of two glioma-devided monocyte chemoattractants[J].J Exp Med,1989,169(4):1449-59
[6]Shyy YJ,Li YS,Kolattukudy PE.Structure of human monocyte chemtactic protein gene and its regulation by TPA[J].Bjochem Biophy Res Common,1990,169(2):346-51
[7]Sica A,Wang JM,Colotta F,et al.Monocyte chemotactic and activating factor gene expression induced in endothelial cells by IL-1 and tumer necrosis factor[J].J Immunol,1990,144(8):3034-8
[8]曾钧,宋烈杰.单核细胞趋化蛋白-1与炎性疾病的研究现状.第一军医大学学报 2001;21(12):84-86
[9]Rollins BJ.et al.Blood.1991:78(4):1112-1116
[10]Shyy YJ.et al.J Imunol.1993:92:1745-1751
Ill]Ward SG,West wick J.Chemokines understanding their role in T2 lymphocyte biology[J].Biochem J,1998,333:457-470
[12]Neote K.Cell,1993;72:415-425
[13]Murphy PM.Cytokine Growth Factor.Rev,1996;7:47-64
[14]刘杰,孙晗笑.趋化因子及其受体转导途径与肿瘤的生长.Chemistry of life 2003;23(1):38-40
[15]赵志勇,周予兰,王伟.等.肿瘤坏死因子对糖尿病视网膜病变的珍断价值[J]中国误诊学杂志 2007,7(1)65:66
[16]宋鄂.王秋利,杨威,等.碱性成纤维细胞生长因子在糖尿病大鼠视网膜血管中的表达[J]眼科研究,2004,22,(3)290:293
[17]郑建秋,郭庚,腾岩.糖尿病大鼠视网膜早期病变组织中生长因子作用的实验研究[J].中华实用眼科杂志 2001,7,(19)494:497
[18]Mrudula T,Suryanarayana P,Srinivas PN,et al.Effect of curcumin on hyperglycemia-induced vascular endothelial growth factor expression in streptozotocin-induced diabetic rat retina.[J]Biochem Biophys Res Commun,2007,Sep 21:361(2):528-532
[19]Chung HS,Harris A,Halter PJ,et al.Regional differences in retinal vascular reactivity[J].Invest Ophthalmol Vis Sci 1999;40:2448-2453
[20]Banas B,Luckow B,Moller M,et al.Chemokine and chemokine receptor expression in novel human mesangial cell line[J].J Am Soc Nephrol 1999;10(11):2314-2322
[21]Ward SG,Westwick J.Chemokines understanding their role in T2 lymphocyte biology[J].Biochem J 1998;333:457-470
[22]Matsumoto Y.TakahashiM,Ogata M.Relationship between glycoxidation and cytokine in the vitreous of eye with diabetic retinopathy[J].Jpn J Ophthalmol 2002:46:406-412
[23]Mitamura Y,Takeuchi S,Matsuda A,et al.Monocyte chemotactic protein-1 in the vitreous of patients with proliferative diabetic retinopathy[J]Ophthalmologica 2001;215:415-418
[24]Abuel Asrar AM,Van Damme J,Put W,et al.Monocyte chemotactic protein-1 in proliferative vitreoretinal disorders[J].Am J Ophthalmol 1997:12:599-606
[25]Marumo T.Schini Kerth V B,Busse R.Vascular endothelial growth factor activates nuclear factor kappaβ and induces monocyte chemoattractant protein-1in bovine retinal endothelial cells[J]Diabetes 1999;48:1131-1137
[26] Sozzani S, Locat M, Zhou D, et al. Recep tors, signal transduction, and spectrum of action of monocyte chemotactic protein-1 and related chemokines[J]. J Leukocyte Biol 1995; 57:788 - 794
[27] Libby P, Sukhova G, Lee RT, et al. Cytokines regulate vascular functions related to stability of the atherosclerotic plaque [J]. J Cardiovasc Pharmacol 1995; 25:S9-S12
[28] Viberti GC, Earle K. Predisposition to essential hypertension and the development of diabetic nephropathy. J Am Soc Nephrol. 1992;3:S27-S33
[29] Klein BE, Moss SE, Klein R, et al. The Wisconsin epidemiologic study of diabetic retinopathy. J Am Soc Nephrol. 1997;4:S28-S35
[30] Antonia M, Oussen, Toshinori Murata, et al. Leukocyte-Mediated Endothelial Cell Injury And Death in the Diabetic Retina. Am J Pathol, 2001,158:147-152
[31] Cohen MP . Clements RS . Cohen JA ,et al.Relationship of serum cholesterol to retinopethy and hard exudates[J]. Ophthalmology, 1991, 98(8)11-61
[32] Kato S ,Luyckx V A , Ots M,et al.monocyte chemoattractant protein-1 in cytokine treated human vascular endothelial cells [J]. FEB S Lett 1999; 454(1-2):27-30
[33] Kato S ,Luyckx V A , Ots M.et al. Reninangiotensin blockade lowers MCP-1 expression in diabetic rats[J].Kidney Int 1999;56 :1037-1048
[34] Cohen MP , Clements RS . Cohen JA ,et al. Prevention of d in the diabetic db/ db mouse[J ] .Diabetologia996;39:270-274
[35] Hagedorn M, Zilberberg L . Lozano R M. et al. A short peptide domain of platelet factor 4 blocks angiogenic key events induced by FGF22[J].FASEB J 2001;15:550-552