原发性高血压患者血浆一氧化碳和硫化氢水平变化的临床意义
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摘要
目的:探讨气体信号分子一氧化碳(CO)及硫化氢(H2S)在原发性高血压患者中的意义。
方法:选择原发性高血压患者85例,按照血压水平分3组,均无服用降压药物、调脂药物或已停服降压药1月以上,排除继发性高血压、糖尿病、冠心病、高脂血症等。同时选取正常对照组27例。分别用Chalmers法和去蛋白法检测入选者的血浆CO及H2S浓度。采用SPSS13.0统计软件进行数据分析,先对数据进行正态性检验,对符合正态性分布的数据以x±s表示;组间比较经方差分析再进行两两比较,通过直线相关分析,以P<0.05为差异有统计学意义。
结果: 1级高血压病人血浆CO含量与对照组比较([18.6±1.9)v(s21.1±2.4)mmol/L,P>0.05],差异无统计学意义。2、3级高血压病人血浆CO含量较对照组降低[(15.4±1.4)、(12.5±1.7) vs (21.1±2.4)mmol/L,P<0.05],差异有统计学意义;1、2、3级高血压病人血浆H2S含量较对照组降低[(43.7±1.4)、(37.6±1.8)、(26.7±1.3)vs(51.4±1.4)mmol/L,P<0.05],差异有统计学意义;随血压水平升高,血浆CO及H2S含量降低,原发性高血压患者血浆CO与H2S成正相关(r=0.627,P<0.05)。
结论:内源性C O和H2S在原发性高血压的发生和发展中均有重要作用。
Objective To study the implication of carbon monoxide (CO) and hydrogen sulfide (H2S) in essential hypertensive patients.
Methods A total of 85 essential hypertensive patients were enrolled in this study and divided into 3 groups according to hypertension grouping criteria. All of them have been stopped from taking hypotensor and antilipemic for over one month or never, such circumstances bases on the ignoring of secondary hypertension, diabetes mellitus, coronary heart disease, hyperlipemia, etc. A total of 27 healthy were also enrolled as comparison. Plasma CO content and H2S concentration were determined in all patients and controls.
Results①Compared with controls, plasma CO level have no marked difference between grade 1 and controls([18.6±1.9)v(s21.07±2.44)mmol/L,P>0.05], but in the grade 2 and 3 was significantly lower [(15.4±1.4)、(12.5±1.7)mmol/L vs (21.1±2.4)mmol/L,P<0.05];②All of plasma H2S level in the three groups are lower than controls[(43.7±1.4)、(37.6±1.8)、(26.7±1.3)vs(51.4±1.4)mmol/L,P<0.05];③Plasma CO and H2S decrease with the increasing of blood pressure and they are positive correlation(r=0.627,P<0.05) in essential hypertensive patients.
Conclusion As a signaling gasotransmitters, plasma CO and H2S might play important roles in the pathogenesis and development of hypertension.
方法:选择原发性高血压患者85例,按照血压水平分3组,均无服用降压药物、调脂药物或已停服降压药1月以上,排除继发性高血压、糖尿病、冠心病、高脂血症等。同时选取正常对照组27例。分别用Chalmers法和去蛋白法检测入选者的血浆CO及H2S浓度。采用SPSS13.0统计软件进行数据分析,先对数据进行正态性检验,对符合正态性分布的数据以x±s表示;组间比较经方差分析再进行两两比较,通过直线相关分析,以P<0.05为差异有统计学意义。
结果: 1级高血压病人血浆CO含量与对照组比较([18.6±1.9)v(s21.1±2.4)mmol/L,P>0.05],差异无统计学意义。2、3级高血压病人血浆CO含量较对照组降低[(15.4±1.4)、(12.5±1.7) vs (21.1±2.4)mmol/L,P<0.05],差异有统计学意义;1、2、3级高血压病人血浆H2S含量较对照组降低[(43.7±1.4)、(37.6±1.8)、(26.7±1.3)vs(51.4±1.4)mmol/L,P<0.05],差异有统计学意义;随血压水平升高,血浆CO及H2S含量降低,原发性高血压患者血浆CO与H2S成正相关(r=0.627,P<0.05)。
结论:内源性C O和H2S在原发性高血压的发生和发展中均有重要作用。
Objective To study the implication of carbon monoxide (CO) and hydrogen sulfide (H2S) in essential hypertensive patients.
Methods A total of 85 essential hypertensive patients were enrolled in this study and divided into 3 groups according to hypertension grouping criteria. All of them have been stopped from taking hypotensor and antilipemic for over one month or never, such circumstances bases on the ignoring of secondary hypertension, diabetes mellitus, coronary heart disease, hyperlipemia, etc. A total of 27 healthy were also enrolled as comparison. Plasma CO content and H2S concentration were determined in all patients and controls.
Results①Compared with controls, plasma CO level have no marked difference between grade 1 and controls([18.6±1.9)v(s21.07±2.44)mmol/L,P>0.05], but in the grade 2 and 3 was significantly lower [(15.4±1.4)、(12.5±1.7)mmol/L vs (21.1±2.4)mmol/L,P<0.05];②All of plasma H2S level in the three groups are lower than controls[(43.7±1.4)、(37.6±1.8)、(26.7±1.3)vs(51.4±1.4)mmol/L,P<0.05];③Plasma CO and H2S decrease with the increasing of blood pressure and they are positive correlation(r=0.627,P<0.05) in essential hypertensive patients.
Conclusion As a signaling gasotransmitters, plasma CO and H2S might play important roles in the pathogenesis and development of hypertension.
引文
[1] Sabaawy HE,Zhang F,Nguyen X, et al. Human heme oxygenase-1 gene transfer lowers blood pressure and promotes growth in spontaneously hypertensive rats [J]. Hypertersion, 2001,38(2):210-215.
[2]杜军保,陈晓波,耿彬,等.硫化氢作为心血管信号分子的研究.北京大学学报(医学版),2002,34:187.
[3] Christopher TW,William D, Nick C,et al. Hemodymamic forces induce the expression of hemeoxygenase in cultured vascular smooth muscle cells [J].J Clin Invest, 1997,100:589-596.
[4] Moore PK, Bhatia M, Moochhala S. Hydrogen sulphide: from the smell of the past to the gas of the future? [J].Trends Pharmacol Sci, 2003,24: 609-611.
[5] Carmine Z, Concetta C,Stefania R .Blood pressure control:hydrogen sulfide, a new gasotransmitter, takes stage [J]. Nephrology Dialysis Transplantation, 2009, 24: 1394-1396.
[6]中国高血压防治指南修订委员会.中国高血压防治指南[J].高血压杂志,2005,13(增刊):2-42.
[7] Hirooka Y, Kishi T, Sakai K, et al. Effect of over production NO In the brain stemonthe cardiovascular response in conscious rats.[J] Cardiovasc Pharmacol,2003, 41(suppl.1):119-126.
[8] Maines M D. The heme oxygenase system: a regulator of second messenger gas [J].Annu Rev Pharmacol Toxicol,1997,37:517.
[9] Shinoda Y, Suematsu M,Wakabayashi Y,et al.Carbon monoxide as a regulation of ribile canal rular contractility in cultured rat hepatocytes [J]. Hepatology, 1998, 28(2):286.
[10] Johnson RA,Colombari E, ColombariDSA,et al. Role of endogenous carbon monoxide in central regulation of arterial pressure [J].Hypertension,1997,30(4): 962-967.
[11] Kostoglou A I,Coste A ,Navarra P,etal. Endotox in stimulates an endogenouspathway regulating corticotrop in-releasing hormone and vaseopress in release involving the generation of nitric oxide and carbon monoxide[J].J Nouroimmunol, 1998.86(1):104-109.
[12]程友琴,武珊珊,王士雯,等.内源性一氧化碳对内皮素-1诱导的大鼠血管平滑肌细胞增殖的抑制作用[J].中华心血管病杂志.1998,26(3);217.
[13] Seki T,Naruse M,Naruse K,etal. Induction of heme oxygenase produces load-independent cardioprotective effects in hypertensive rats[J].Life Sci, 1999, 65(10):1077.
[14] Aizawa T,Ishizaka N,Taguchi T,etal. Heme oxygenase-1 is up regulated in the kidney of angiotensinⅡ-induced hypertension rats[J]. Hypertension, 2000, 35 (3):800.
[15] Doeller JE,Isbell TS. Polarographic measurement of hydrogen sulfide production and consumption by mammalian tissues. Anal Biochem, 2005,341,40 3-51.
[16] Furne J, Springfield J,Koenig T, etal .Oxidation of hydrogensulfide and methanethiol to thiosulfate by rat tissues: A specialized function of the colonic mucosa[J].BiochemPharmacol,2001,62 (2) :255-259.
[17] Zhao WM,Zhang J,Lu YJ et al. The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener. EMBO [J],2001;20(21):6008-6016.
[18] Zhao WM,Wang R. H2S induced vasorelaxtion and under lying cellular and molecular mechanisms[J].AmJ Physiol Heart Circ Physiol,2002,283,(2):474- 480.
[19] Moore PK, Bhatia M, Moochhala S. Hydrogen sulphide: from the smell of the past to the gas of the future? [J].Trends Pharmacol Sci, 2003,24: 609-611.
[20] Carmine Z, Concetta C,Stefania R .Blood pressure control:hydrogen sulfide, a new gasotransmitter, takes stage [J]. Nephrology Dialysis Transplantation, 2009, 24: 1394-1396.
[21] Yan H,Du JB,Tang CS. The possible role of hydrogen sulfide on the pathogenesis of spontaneous hypertension in rats [J].Biochem Biophys Res Commun, 2004,313(1):22-27.
[22] Mustafa AK, Gadalla MM, Snyder SH.Signaling by asotransmitters[J]. Sci. Signal,2009: 121-124.
[23]闫辉,杜军保,唐朝枢.硫化氢对自发性高血压大鼠主动脉平滑肌细胞增殖与凋亡的影响[J].实用儿科临床杂志,2004,19(13):188-190.
[24]耿彬,杨靖辉,庞永正,等.硫化氢对大鼠离体灌注心脏心功能的影响[J].中国病理生理学杂志,2005,21(1);1-5.
[25]金红芳,杜军保,唐朝枢,等.一氧化碳下调高血压大鼠血管平滑肌细胞硫化氢体系[J].实用儿科临床杂志,2005,20(12):1179-1180.
[26] Charles WL, Helena Parfenova, Jonathan H, et al. Carbon monoxide and hydrogen sulfide: gaseous messengers in cerebrovascular circulation [J]. Appl. Physiol. 2006,100: 1065-1076.
[27] Chalmers AH. Simple, sensitive measurement of carbon monoxide in plasma. Clin Chem,1991,37:1442-1445.
[28] Chunyu Z, Junbao D, Dingfang B, et al. The regulatory effect of hydrogen sulfide on hypoxic pulmonary hypertension in rats. Biochem Biophys Res Commun, 2003,302:810-816.
[1] Doeller JE,Isbell TS. Polarographic measurement of hydrogen sulfide production and consumption by mammalian tissues. Anal Biochem, 2005;341,40-51.
[2] Zhao WM,Joseph FN,Wang R. Modulation of endogenous production of H2S in rat tissues. Can J Physio Phamacol,2003;81(9):848-853.
[3] Geng B,Yang J,Qi Y et al. H2S generated by heart in rat and its effects on cardiac function. Biochem Biophys Res Commun,2004;313(2):362-368.
[4] Zhao WM,Zhang J,Lu YJ et al. The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener. EMBO [J],2001;20(21):6008-6016.
[5] Sabaawy HE,Zhang F,Nguyen X, et al. Human heme oxygenase-1 gene transferlowers blood pressure and promotes growth in spontaneously hypertensive rats [J]. Hypertersion, 2001,38(2):210-215.
[6] Wang R Two's company, three's a crowd: can H2S be the third endogenous gaseous transmitter? FASEB [J],2002,16:1792-1798.
[7]杜军保,陈晓波,耿彬,等.硫化氢作为心血管信号分子的研究.北京大学学报(医学版),2002,34:187.
[8] Hosoki R,Matsuki N,Kimura H The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide. Biochem Biophys Res Commun,1997,237:527-531.
[9]刘皋林一氧化氮与心血管疾病[J].药理学进展,2001,23(5):101-110.
[10] Hirooka Y, Kishi T, Sakai K, et al. Effect of over production NO In the brain stemonthe cardiovascular response in conscious rats.[J] Cardiovasc Pharmacol,2003, 41(suppl.1):119-126.
[11] Hosoki R,Matsuki N,KimuraH. The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide [J].Biochem Biophys Res Commun,1997,237(3):527-531
[12] Zhao WM,Wang R. H2S induced vasorelaxtion and under lying cellular and molecular mechanisms[J].AmJ Physiol Heart Circ Physiol, 2002,283, (2): 474-480.
[13]闫辉,杜军保,唐朝枢.硫化氢对自发性高血压大鼠主动脉平滑肌细胞增殖与凋亡的影响[J].使用儿科临床杂志,2004,19(13):188-190.
[14] Yan H,Du JB,Tang CS. The possible role of hydrogen sulfide on the pathogenesis of spontaneous hypertension in rats [J].Biochem Biophys Res Commun,2004, 313(1):22-27.
[15] EtoK, KimuraH.The production of hydrogen sulfide is regulated by testosterone and S-adenosyl-L-methioninein mouse brain [J].Neurochem,2002,83 (1):80-86.
[16]耿彬,杨靖辉,庞永正,等.硫化氢对大鼠离体灌注心脏心功能的影响[J].中国病理生理学杂志,2005,21(1):1-5.
[17] Gross GJ,Fryer RM.Sarcolemmal versus mitochondrial ATP-senstive K+ channels and myocardial preconditioning[J].CircRes,1999,84(9):973-979.
[18] GengB,YangJH,QiY,et al.H2S generated by heart in rat and its effect on cardiacfunction [J].Biochem Biophys Res Commun,2004,313(2):362-368.
[19] Maines M D. The heme oxygenase system: a regulator of second messenger gas [J].Annu Rev Pharmacol Toxicol,1997,37:517.
[20]杜军保,齐建光,石云,等.肺动脉高压形成机制中的气体信号分子网络.北京大学学报(医学版),2002,34(5):536-541.
[21] Gaine S P,Booth G ,Otterem L,et al.Induction of hemeoxygenase-1 with hemoglobin depresses vasoreactivity in rat aorta[J]. J Vase Res,1999,36(2):114
[22] Christodoulides N,Durante W,Kroll M H,et al.Vascular smooth muscle cell heme oxygenase generate guanly cyclase-stimulatory carbon monoxide[J]. Cirulation, 1995,91(9):2306.
[23] Suematse M, Wakabayashi Y, Ishimara Y. Gaseous monoxides, a new class of microvascular regulator in the liver[J].Cardiovasc Res,1996,32(4):679
[24] Shinoda Y, Suematsu M,Wakabayashi Y,et al. Carbon monoxide as a regulation of ribile canal rular contractility in cultured rat hepatocytes [J]. Hepatology, 1998, 28(2):286.
[25] Johnson RA,Colombari E, ColombariDSA,etal. Role of endogenous carbon monoxide in central regulation of arterial pressure [J].Hypertension, 1997, 30 (4):962-967.
[26] Kostoglou A I,Coste A ,Navarra P,etal. Endotox in stimulates an endogenous pathway regulating corticotrop in-releasing hormone and vaseopress in release involving the generation of nitric oxide and carbon monoxide[J].J Nouroimmunol, 1998.86(1):104-109.
[27] Koourembanas S,Morita T,Lia t, et al. Mechanism by which oxygen regulate gene expression and cell-cell interaction in the vascular [J].Kidney Int, 1997, 51 (2): 438.
[28]程友琴,武珊珊,王士雯,等.内源性一氧化碳对内皮素-1诱导的大鼠血管平滑肌细胞增殖的抑制作用[J].中华心血管病杂志,1998,26(3);217.
[29] Seki T,Naruse M,Naruse K,etal. Induction of heme oxygenase produces load-independent cardioprotective effects in hypertensive rats[J].Life Sci,1999, 65(10):1077.
[30] Aizawa T,Ishizaka N,Taguchi T,etal. Heme oxygenase-1 is up regulated in the kidney of angiotensinⅡ-induced hypertension rats[J]. Hypertension, 2000, 35 (3): 800.
[31] Zhang Q Y,Du JB,Zhou WJ,et al. Impact of hydrogen sulfide on carbon monoxide oxygenase pathway in the pathogenesis of hypoxic pulmonary hypertension[J]. Biochem Biophys Res Commun,2004,317:30-37.
[32] Ushiyama M, Morita T,Katayama S.Carbon monoxide regulates blood pressure cooperatively with nitric oxide in hypertensive rats[J].Heart Vessels, 2002,16(5): 189-195.
[33]金红芳,杜军保,唐朝枢,等.一氧化碳下调高血压大鼠血管平滑肌细胞硫化氢体系[J] .实用儿科临床杂志,2005,20(12):1179-1180.
[34] Hosoki Matsuki N, Kimura H.The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide [J].Biochem Biophy Res Commun,1997,237(3):527-531.
[35] Gong FG,Shiraishi H, Mariko Y. The effect of inhalation mebulized nitrogly cerinin dogs with experimental pulmonary hypertension induced by U46619 [J] .Chin Med, 2000,113(5):475-476.
[36] Zhao W, Wang R. H2S-induced vasorelaxantion and underlying cellular and molecular mechanisms[J].Am J Physiol Heart Circ Physiol,2002,283(2): H474- 480.
[37]范瑾,杜军保,何晓菊.雾化吸入硝酸甘油对先天性心脏病并肺动脉高压患儿血流动力学的影响「J] .实用儿科临床杂志2001,16(6):389- 391.
[38]张清友,杜军保,张春雨,等.内源性一氧化氮对低氧大鼠肺动脉硫化氢胱硫醚-γ-裂解酶体系的影响[J].实用儿科临床杂志2003,18(11):865.867.
[39] Wang R. The gasotransmitter role of hydrogen sulfide [J].Antioxid Redox Singnal,2003,(4):493-501.
[40] Geng B,Yang JH,Qi YF,etal.H2S generated by heart in rat and it effect on cardiac function[J].Biochem Biophy Res Commun,2004,313(2):362-368.
[41] Abe K,Kimura H. The possible role of hydrogen sulfide as an endogenous neuromodulator[J].J Neuro sci,1996,16(3):10661-1071.
[42] Morita T , Perrella MA , Lee M-E , et al . Smooth muscle cell-derived carbon monoxide is a regulator of vascular cGMP. Proc Natl Acad Sci USA , 1995, 92:1475-1479.
[43]欧和生,严丽梅,等.内源性一氧化碳与一氧化氮在高血压发病中的相互作用[J]北京医科大学学报1999,31:520-523.
[44] Maines MD. Carbon monoxide and nitric oxide homology : differential modulation of heme oxygenase in brain and detection of protein and activity. Methods Enzymol, 1996, 268: 473-488.
[45]欧和生,王晓红,等.内源性一氧化碳与一氧化氮在高血压发病中的相互作用研究(Ⅱ) [J]中国应用生理学杂志2000:16(2):172-176.
[2]杜军保,陈晓波,耿彬,等.硫化氢作为心血管信号分子的研究.北京大学学报(医学版),2002,34:187.
[3] Christopher TW,William D, Nick C,et al. Hemodymamic forces induce the expression of hemeoxygenase in cultured vascular smooth muscle cells [J].J Clin Invest, 1997,100:589-596.
[4] Moore PK, Bhatia M, Moochhala S. Hydrogen sulphide: from the smell of the past to the gas of the future? [J].Trends Pharmacol Sci, 2003,24: 609-611.
[5] Carmine Z, Concetta C,Stefania R .Blood pressure control:hydrogen sulfide, a new gasotransmitter, takes stage [J]. Nephrology Dialysis Transplantation, 2009, 24: 1394-1396.
[6]中国高血压防治指南修订委员会.中国高血压防治指南[J].高血压杂志,2005,13(增刊):2-42.
[7] Hirooka Y, Kishi T, Sakai K, et al. Effect of over production NO In the brain stemonthe cardiovascular response in conscious rats.[J] Cardiovasc Pharmacol,2003, 41(suppl.1):119-126.
[8] Maines M D. The heme oxygenase system: a regulator of second messenger gas [J].Annu Rev Pharmacol Toxicol,1997,37:517.
[9] Shinoda Y, Suematsu M,Wakabayashi Y,et al.Carbon monoxide as a regulation of ribile canal rular contractility in cultured rat hepatocytes [J]. Hepatology, 1998, 28(2):286.
[10] Johnson RA,Colombari E, ColombariDSA,et al. Role of endogenous carbon monoxide in central regulation of arterial pressure [J].Hypertension,1997,30(4): 962-967.
[11] Kostoglou A I,Coste A ,Navarra P,etal. Endotox in stimulates an endogenouspathway regulating corticotrop in-releasing hormone and vaseopress in release involving the generation of nitric oxide and carbon monoxide[J].J Nouroimmunol, 1998.86(1):104-109.
[12]程友琴,武珊珊,王士雯,等.内源性一氧化碳对内皮素-1诱导的大鼠血管平滑肌细胞增殖的抑制作用[J].中华心血管病杂志.1998,26(3);217.
[13] Seki T,Naruse M,Naruse K,etal. Induction of heme oxygenase produces load-independent cardioprotective effects in hypertensive rats[J].Life Sci, 1999, 65(10):1077.
[14] Aizawa T,Ishizaka N,Taguchi T,etal. Heme oxygenase-1 is up regulated in the kidney of angiotensinⅡ-induced hypertension rats[J]. Hypertension, 2000, 35 (3):800.
[15] Doeller JE,Isbell TS. Polarographic measurement of hydrogen sulfide production and consumption by mammalian tissues. Anal Biochem, 2005,341,40 3-51.
[16] Furne J, Springfield J,Koenig T, etal .Oxidation of hydrogensulfide and methanethiol to thiosulfate by rat tissues: A specialized function of the colonic mucosa[J].BiochemPharmacol,2001,62 (2) :255-259.
[17] Zhao WM,Zhang J,Lu YJ et al. The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener. EMBO [J],2001;20(21):6008-6016.
[18] Zhao WM,Wang R. H2S induced vasorelaxtion and under lying cellular and molecular mechanisms[J].AmJ Physiol Heart Circ Physiol,2002,283,(2):474- 480.
[19] Moore PK, Bhatia M, Moochhala S. Hydrogen sulphide: from the smell of the past to the gas of the future? [J].Trends Pharmacol Sci, 2003,24: 609-611.
[20] Carmine Z, Concetta C,Stefania R .Blood pressure control:hydrogen sulfide, a new gasotransmitter, takes stage [J]. Nephrology Dialysis Transplantation, 2009, 24: 1394-1396.
[21] Yan H,Du JB,Tang CS. The possible role of hydrogen sulfide on the pathogenesis of spontaneous hypertension in rats [J].Biochem Biophys Res Commun, 2004,313(1):22-27.
[22] Mustafa AK, Gadalla MM, Snyder SH.Signaling by asotransmitters[J]. Sci. Signal,2009: 121-124.
[23]闫辉,杜军保,唐朝枢.硫化氢对自发性高血压大鼠主动脉平滑肌细胞增殖与凋亡的影响[J].实用儿科临床杂志,2004,19(13):188-190.
[24]耿彬,杨靖辉,庞永正,等.硫化氢对大鼠离体灌注心脏心功能的影响[J].中国病理生理学杂志,2005,21(1);1-5.
[25]金红芳,杜军保,唐朝枢,等.一氧化碳下调高血压大鼠血管平滑肌细胞硫化氢体系[J].实用儿科临床杂志,2005,20(12):1179-1180.
[26] Charles WL, Helena Parfenova, Jonathan H, et al. Carbon monoxide and hydrogen sulfide: gaseous messengers in cerebrovascular circulation [J]. Appl. Physiol. 2006,100: 1065-1076.
[27] Chalmers AH. Simple, sensitive measurement of carbon monoxide in plasma. Clin Chem,1991,37:1442-1445.
[28] Chunyu Z, Junbao D, Dingfang B, et al. The regulatory effect of hydrogen sulfide on hypoxic pulmonary hypertension in rats. Biochem Biophys Res Commun, 2003,302:810-816.
[1] Doeller JE,Isbell TS. Polarographic measurement of hydrogen sulfide production and consumption by mammalian tissues. Anal Biochem, 2005;341,40-51.
[2] Zhao WM,Joseph FN,Wang R. Modulation of endogenous production of H2S in rat tissues. Can J Physio Phamacol,2003;81(9):848-853.
[3] Geng B,Yang J,Qi Y et al. H2S generated by heart in rat and its effects on cardiac function. Biochem Biophys Res Commun,2004;313(2):362-368.
[4] Zhao WM,Zhang J,Lu YJ et al. The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener. EMBO [J],2001;20(21):6008-6016.
[5] Sabaawy HE,Zhang F,Nguyen X, et al. Human heme oxygenase-1 gene transferlowers blood pressure and promotes growth in spontaneously hypertensive rats [J]. Hypertersion, 2001,38(2):210-215.
[6] Wang R Two's company, three's a crowd: can H2S be the third endogenous gaseous transmitter? FASEB [J],2002,16:1792-1798.
[7]杜军保,陈晓波,耿彬,等.硫化氢作为心血管信号分子的研究.北京大学学报(医学版),2002,34:187.
[8] Hosoki R,Matsuki N,Kimura H The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide. Biochem Biophys Res Commun,1997,237:527-531.
[9]刘皋林一氧化氮与心血管疾病[J].药理学进展,2001,23(5):101-110.
[10] Hirooka Y, Kishi T, Sakai K, et al. Effect of over production NO In the brain stemonthe cardiovascular response in conscious rats.[J] Cardiovasc Pharmacol,2003, 41(suppl.1):119-126.
[11] Hosoki R,Matsuki N,KimuraH. The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide [J].Biochem Biophys Res Commun,1997,237(3):527-531
[12] Zhao WM,Wang R. H2S induced vasorelaxtion and under lying cellular and molecular mechanisms[J].AmJ Physiol Heart Circ Physiol, 2002,283, (2): 474-480.
[13]闫辉,杜军保,唐朝枢.硫化氢对自发性高血压大鼠主动脉平滑肌细胞增殖与凋亡的影响[J].使用儿科临床杂志,2004,19(13):188-190.
[14] Yan H,Du JB,Tang CS. The possible role of hydrogen sulfide on the pathogenesis of spontaneous hypertension in rats [J].Biochem Biophys Res Commun,2004, 313(1):22-27.
[15] EtoK, KimuraH.The production of hydrogen sulfide is regulated by testosterone and S-adenosyl-L-methioninein mouse brain [J].Neurochem,2002,83 (1):80-86.
[16]耿彬,杨靖辉,庞永正,等.硫化氢对大鼠离体灌注心脏心功能的影响[J].中国病理生理学杂志,2005,21(1):1-5.
[17] Gross GJ,Fryer RM.Sarcolemmal versus mitochondrial ATP-senstive K+ channels and myocardial preconditioning[J].CircRes,1999,84(9):973-979.
[18] GengB,YangJH,QiY,et al.H2S generated by heart in rat and its effect on cardiacfunction [J].Biochem Biophys Res Commun,2004,313(2):362-368.
[19] Maines M D. The heme oxygenase system: a regulator of second messenger gas [J].Annu Rev Pharmacol Toxicol,1997,37:517.
[20]杜军保,齐建光,石云,等.肺动脉高压形成机制中的气体信号分子网络.北京大学学报(医学版),2002,34(5):536-541.
[21] Gaine S P,Booth G ,Otterem L,et al.Induction of hemeoxygenase-1 with hemoglobin depresses vasoreactivity in rat aorta[J]. J Vase Res,1999,36(2):114
[22] Christodoulides N,Durante W,Kroll M H,et al.Vascular smooth muscle cell heme oxygenase generate guanly cyclase-stimulatory carbon monoxide[J]. Cirulation, 1995,91(9):2306.
[23] Suematse M, Wakabayashi Y, Ishimara Y. Gaseous monoxides, a new class of microvascular regulator in the liver[J].Cardiovasc Res,1996,32(4):679
[24] Shinoda Y, Suematsu M,Wakabayashi Y,et al. Carbon monoxide as a regulation of ribile canal rular contractility in cultured rat hepatocytes [J]. Hepatology, 1998, 28(2):286.
[25] Johnson RA,Colombari E, ColombariDSA,etal. Role of endogenous carbon monoxide in central regulation of arterial pressure [J].Hypertension, 1997, 30 (4):962-967.
[26] Kostoglou A I,Coste A ,Navarra P,etal. Endotox in stimulates an endogenous pathway regulating corticotrop in-releasing hormone and vaseopress in release involving the generation of nitric oxide and carbon monoxide[J].J Nouroimmunol, 1998.86(1):104-109.
[27] Koourembanas S,Morita T,Lia t, et al. Mechanism by which oxygen regulate gene expression and cell-cell interaction in the vascular [J].Kidney Int, 1997, 51 (2): 438.
[28]程友琴,武珊珊,王士雯,等.内源性一氧化碳对内皮素-1诱导的大鼠血管平滑肌细胞增殖的抑制作用[J].中华心血管病杂志,1998,26(3);217.
[29] Seki T,Naruse M,Naruse K,etal. Induction of heme oxygenase produces load-independent cardioprotective effects in hypertensive rats[J].Life Sci,1999, 65(10):1077.
[30] Aizawa T,Ishizaka N,Taguchi T,etal. Heme oxygenase-1 is up regulated in the kidney of angiotensinⅡ-induced hypertension rats[J]. Hypertension, 2000, 35 (3): 800.
[31] Zhang Q Y,Du JB,Zhou WJ,et al. Impact of hydrogen sulfide on carbon monoxide oxygenase pathway in the pathogenesis of hypoxic pulmonary hypertension[J]. Biochem Biophys Res Commun,2004,317:30-37.
[32] Ushiyama M, Morita T,Katayama S.Carbon monoxide regulates blood pressure cooperatively with nitric oxide in hypertensive rats[J].Heart Vessels, 2002,16(5): 189-195.
[33]金红芳,杜军保,唐朝枢,等.一氧化碳下调高血压大鼠血管平滑肌细胞硫化氢体系[J] .实用儿科临床杂志,2005,20(12):1179-1180.
[34] Hosoki Matsuki N, Kimura H.The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide [J].Biochem Biophy Res Commun,1997,237(3):527-531.
[35] Gong FG,Shiraishi H, Mariko Y. The effect of inhalation mebulized nitrogly cerinin dogs with experimental pulmonary hypertension induced by U46619 [J] .Chin Med, 2000,113(5):475-476.
[36] Zhao W, Wang R. H2S-induced vasorelaxantion and underlying cellular and molecular mechanisms[J].Am J Physiol Heart Circ Physiol,2002,283(2): H474- 480.
[37]范瑾,杜军保,何晓菊.雾化吸入硝酸甘油对先天性心脏病并肺动脉高压患儿血流动力学的影响「J] .实用儿科临床杂志2001,16(6):389- 391.
[38]张清友,杜军保,张春雨,等.内源性一氧化氮对低氧大鼠肺动脉硫化氢胱硫醚-γ-裂解酶体系的影响[J].实用儿科临床杂志2003,18(11):865.867.
[39] Wang R. The gasotransmitter role of hydrogen sulfide [J].Antioxid Redox Singnal,2003,(4):493-501.
[40] Geng B,Yang JH,Qi YF,etal.H2S generated by heart in rat and it effect on cardiac function[J].Biochem Biophy Res Commun,2004,313(2):362-368.
[41] Abe K,Kimura H. The possible role of hydrogen sulfide as an endogenous neuromodulator[J].J Neuro sci,1996,16(3):10661-1071.
[42] Morita T , Perrella MA , Lee M-E , et al . Smooth muscle cell-derived carbon monoxide is a regulator of vascular cGMP. Proc Natl Acad Sci USA , 1995, 92:1475-1479.
[43]欧和生,严丽梅,等.内源性一氧化碳与一氧化氮在高血压发病中的相互作用[J]北京医科大学学报1999,31:520-523.
[44] Maines MD. Carbon monoxide and nitric oxide homology : differential modulation of heme oxygenase in brain and detection of protein and activity. Methods Enzymol, 1996, 268: 473-488.
[45]欧和生,王晓红,等.内源性一氧化碳与一氧化氮在高血压发病中的相互作用研究(Ⅱ) [J]中国应用生理学杂志2000:16(2):172-176.