桃PpERS1、PpADC基因克隆、鉴定及PtADC转基因功能分析
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摘要
桃树是全球广泛栽培的重要果树之一。新品种的选育和推广应用是促进桃产业健康持续发展的基础。基因工程技术由于目的性强、时间短等优点使其成为当前育种学家常用的手段之一,发掘植物体内的优良基因资源是应用基因工程技术创造植物新种质的必要前提。桃果实独特的成熟衰老进程,以及果实成熟期的生理生化物质代谢机制研究的深入,使桃树成为果树植物中研究成熟和基因组分析的模式植物。同时,桃树对土壤条件要求不太严格,栽培地域限制较小,为育种学家培育抵抗多重逆境胁迫的植物新品种提供了良好的种质资源。本研究从桃中成功克隆到PpERS1和PpADC基因及其启动子,并对它们进行了转基因功能验证,同时通过验证柑橘PtADC基因在转基因番茄抗旱过程中的功能,拓展了ADC基因在植物体内的分子生物学功能。主要结果如下：
1.采用同源序列法从桃树叶片中克隆到乙烯受体基因PpERS1,基因序列包含1935bp的开放阅读框,编码644个氨基酸。其编码蛋白质分子量预测为72.4kDa,等电点为6.33。多序列比对和系统进化树分析表明PpERS1属于乙烯受体基因家族的ETR1亚家族,与蔷薇科植物的ERS1基因具有高度的同源性。生物信息学分析表明PpERS1基因开放阅读框含有5个外显子和4个内含子,此外,基因5'UTR序列中含有2个内含子,第1个内含子存在选择性剪切机制。基因表达分析表明PpERS1基因在桃树根、茎、功能叶片、花、幼果和成熟果实中均能表达,其中根组织中PpERS1基因的表达量最低,茎、功能叶片、花、幼果和成熟果实中PpERS1基因表达量分别是根组织的1.5、1.8、2.4、1.5和4.3倍。逆境胁迫条件下,PpERS1基因受到伤害、脱水、乙烯利、高盐和桃流胶病菌侵染的诱导表达,受到低温处理的抑制表达。
2.应用染色体步移技术克隆到PpERS1基因启动子,其全长为2798bp.5'RACE结果表明转录起始位点为G碱基,位于翻译起始位点ATG上游-777bp处,生物信息学分析表明启动子序列含有典型的TATA box和CAAT box,并且含有乙烯、伤害和增强子响应元件,以及生长素、分裂素、脱落酸和赤霉素等激素响应元件,干旱、低温、病原菌等逆境胁迫响应元件。通过构建启动子全长和5’端系列缺失的GUS植物表达载体,并将其转化Micro-Tom番茄,GUS组织化学染色和荧光定量分析均表明启动子具有正常的启动子活性,并且启动子序列中内含子缺失不影响正常的启动子活性。不同组织器官和发育阶段GUS染色结果表明启动子在转基因番茄的根、茎、叶、花和果实中均能正常表达,在种子萌发过程中的胚根、上胚轴、子叶和真叶中也能正常表达,同时受到伤害处理诱导表达,GUS定量分析表明启动子受到伤害和乙烯利诱导表达,受到低温抑制表达,高盐处理不影响启动子的活性。
3.采用同源序列法从桃中克隆到精氨酸脱羧酶基因PpADC,基因全长包含2178bp的开放阅读框,编码725个氨基酸。其编码蛋白质分子量预测为77.7kDa,等电点为5.19。多序列比对和系统进化树分析表明PpADC与苹果等蔷薇科植物的ADC基因具有高度的同源性。生物信息学分析表明PpADC基因没有内含子结构。逆境胁迫条件下,PpADC基因受到脱水、乙烯利、高盐和低温处理的诱导表达。将PpADC基因超量表达载体转化番茄Micro-Tom,转基因植株中PpADC基因超量表达,内源腐胺、亚精胺和精胺含量相对于野生型番茄明显提高,转基因植株生长发育迟缓、开花期延迟,外源施用赤霉素能有效恢复转基因植株生长势,基因表达分析表明转基因植株部分赤霉素合成相关基因表达明显受到抑制。
4.应用染色体步移和电子克隆技术获得PpADC基因启动子,其全长为2043bp。生物信息学分析表明启动子的转录起始位点为A碱基,位于翻译起始位点ATG上游-453bp处,其序列含有典型的TATA box,并且含有干旱、低温、病原菌等逆境胁迫响应元件,以及生长素、伤害和生物钟调控响应元件。通过构建启动子全长和5’系列缺失的GUS植物表达载体,遗传转化Micro-Tom番茄,GUS组织化学染色分析表明PpADC启动子具有正常的启动子活性。
5.PtADC转基因功能分析结果表明,转基因番茄中PtADC基因超量表达,转基因植株中内源腐胺和亚精胺含量相对于野生型番茄明显提高,但内源精胺含量没有明显变化。离体叶片脱水处理过程中转基因植株的失水率明显低于野生型番茄。处理结束时,形态学观察表明野生型番茄离体叶片相对于转基因植株明显萎蔫,转基因植株离体叶片的相对电导率明显低于野生型番茄,而叶绿素含量与相对电导率表现相反结果,并且NBT和DAB染色结果表明转基因植株ROS含量明显低于野生型番茄。干旱处理导致野生型番茄萎蔫程度明显高于转基因植株。
Peach is one of the most important economic fruit trees among the world. The release and promotion of new cultivar underpin the healthy and sustainable development of peach industry. Genetic engineering is a feasible approach for cultivar breeding because of the sound technique foundation and high efficiency. But discovery of the desirable genes should be the prerequisite. Peach enjoys the unique characteristics of ripening and the accompanying biochemical mechanism has been studied intensively. Therefore, it has become the model for ripening study in fruit tree. In addition, peach is of no exception concerning stress restriction, which has provoked breeders throughout the world to create new cultivars with tolerance to multiple stresses in breeding program. In the current study, PpERS1and PpADC genes along with their respective promoters were cloned from Prunus persica and transferred into Micro-Tom tomato for functional analysis. Meanwhile, the transgenic tomato carrying PtADC were used to investigate its function under dehydration and drought stress. Main results were as follows:
1. Based on the homogenous sequences, an ethylene receptor gene PpERSl was cloned from peach, containing an open reading frame (ORF) of1935bp which encodes a644amino acids polypeptide with a predicted molecular mass of72.4kDa and an isoelectric point of6.33. Analysis of the putative amino acid sequence suggests that PpERS1was clustered to into the ETR1subfamily, and showed high homology with those ethylene receptors from rosaceous plants. Bioinformatics analysis revealed that the coding region of PpERS1contained five exons interrupted by four introns, in addition, two introns located in the5'UTR of PpERSl gene, and alternative splicing occurred in the first intron of5'UTR. The expression analysis indicated that the PpERSl transcript was detected in all the tissues including root, stem, leaf, flower, fruitlet and ripening fruit. The expression of PpERSl was lowest in root and the expressions in stem, leaf, flower, fruitlet and ripening fruit were1.5,1.8,2.4,1.5and4.3fold higher to that of root, respectively. PpERSl expression was induced by injury, dehydration, ethephon, salt and peach tree gummosis, while low temperature down-regulated PpERSl transcription.
2. A2798bp upstream region of PpERSl translation start codon (ATG) was cloned by genomic walking PCR.5'RACE result revealed that the transcription start site was G, located at-777bp upstream of ATG of PpERS1. In silico analysis of the PpERS1promoter revealed the presence of typical TATA box and CAAT box, ethylene response element, injury response element, enhancer element, hormone response elements including auxin, cytokinin, abscisic acid and gibberellin, and stress response elements including drought, low temperature and pathogen. We constructed vectors containing the PpERSl full-length and serial5'-deleted promoters with GUS reporter gene, and all the constructions were introduced into tomato (Lycopersicon esculentum cv. Micro-Tom) through Agrobacterium tumefaciens-mediated transformation. GUS activity analysis was carried out by histochemical staining and fluorescence detection demonstrated that the promoter activity was normal in transgenic plants and not affected by the introns contained in5'UTR. GUS activity analysis in tissue, organs and developmental stages indicated the PpERSl promoter was active in almost all tissues. Meanwhile, PpERS1promoter activity was detected in radicle, cotyledons, hypocotyls and euphylla during different development stages. Real Time PCR analysis suggested that promoter activity were induced by injury and ethephon treatment, suppressed by low temperature and immune to salt treatment.
3. Based on the homogenous sequences, an arginine decarboxylase gene PpADC was cloned from peach, containing an open reading frame (ORF) of2178bp which encodes a725amino acids polypeptide with a predicted molecular mass of77.7kDa and an isoelectric point of5.19. Analysis of the putative amino acid sequence suggests that PpADC shared high homology with that from rosaceous plants. Bioinformatics analysis revealed that no intron was contained in the coding region of PpADC. The expression analysis indicated that the PpADC transcription were up-regulated by dehydration, ethephon, salt and low temperature treatment. The transgenic plants overexpressing PpADC contained higher Put, Spd and Spm level than that of the wild type (WT). Meanwhile, the transgenic plants showed dwarfism and late-flowering. However, the phenotype was rescued by gibberellin application. The expression analysis indicated that partial genes associated with gibberellin biosynthesis were suppressed in transgenic plant.
4. A2043bp upstream region of PpADC translation start codon (ATG) was cloned by genomic walking and silicon cloning PCR. In silico analysis of the PpADC promoter revealed that the transcription start site was A, located at-453bp upstream of ATG of PpADC. The sequence of PpADC promoter contained the putative TATA box, stress response elements including drought, low temperature and pathogen, auxin response element, injury response element, and circadian regulation element. Vectors containing the PpADC full-length and serial5'-deleted promoters with GUS reporter gene were constructed and introduced into tomato(Lycopersicon esculentum cv. Micro-Tom) through Agrobacterium tumefaciens-mediated transformation. GUS activity analysis carried out by histochemical staining demonstrated that the promoter activity was normal in transgenic plants.
5. The transgenic plants overexpressing PtADC contained higher Put and Spd level than the WT, while there was no difference in Spm level. Under dehydration, water loss of detached leaves was severer in the WT. Moreover, ion leakage of the transgenic lines was remarkably lower than that of the WT. However, chlorophyll content was higher in the WT. In addition, NBT and DAB histochemical staining revealed that WT accumulated more ROS than the transgenic lines in tomato. Therefore, drought tolerance of the transgenic lines was obviously improved.
1.采用同源序列法从桃树叶片中克隆到乙烯受体基因PpERS1,基因序列包含1935bp的开放阅读框,编码644个氨基酸。其编码蛋白质分子量预测为72.4kDa,等电点为6.33。多序列比对和系统进化树分析表明PpERS1属于乙烯受体基因家族的ETR1亚家族,与蔷薇科植物的ERS1基因具有高度的同源性。生物信息学分析表明PpERS1基因开放阅读框含有5个外显子和4个内含子,此外,基因5'UTR序列中含有2个内含子,第1个内含子存在选择性剪切机制。基因表达分析表明PpERS1基因在桃树根、茎、功能叶片、花、幼果和成熟果实中均能表达,其中根组织中PpERS1基因的表达量最低,茎、功能叶片、花、幼果和成熟果实中PpERS1基因表达量分别是根组织的1.5、1.8、2.4、1.5和4.3倍。逆境胁迫条件下,PpERS1基因受到伤害、脱水、乙烯利、高盐和桃流胶病菌侵染的诱导表达,受到低温处理的抑制表达。
2.应用染色体步移技术克隆到PpERS1基因启动子,其全长为2798bp.5'RACE结果表明转录起始位点为G碱基,位于翻译起始位点ATG上游-777bp处,生物信息学分析表明启动子序列含有典型的TATA box和CAAT box,并且含有乙烯、伤害和增强子响应元件,以及生长素、分裂素、脱落酸和赤霉素等激素响应元件,干旱、低温、病原菌等逆境胁迫响应元件。通过构建启动子全长和5’端系列缺失的GUS植物表达载体,并将其转化Micro-Tom番茄,GUS组织化学染色和荧光定量分析均表明启动子具有正常的启动子活性,并且启动子序列中内含子缺失不影响正常的启动子活性。不同组织器官和发育阶段GUS染色结果表明启动子在转基因番茄的根、茎、叶、花和果实中均能正常表达,在种子萌发过程中的胚根、上胚轴、子叶和真叶中也能正常表达,同时受到伤害处理诱导表达,GUS定量分析表明启动子受到伤害和乙烯利诱导表达,受到低温抑制表达,高盐处理不影响启动子的活性。
3.采用同源序列法从桃中克隆到精氨酸脱羧酶基因PpADC,基因全长包含2178bp的开放阅读框,编码725个氨基酸。其编码蛋白质分子量预测为77.7kDa,等电点为5.19。多序列比对和系统进化树分析表明PpADC与苹果等蔷薇科植物的ADC基因具有高度的同源性。生物信息学分析表明PpADC基因没有内含子结构。逆境胁迫条件下,PpADC基因受到脱水、乙烯利、高盐和低温处理的诱导表达。将PpADC基因超量表达载体转化番茄Micro-Tom,转基因植株中PpADC基因超量表达,内源腐胺、亚精胺和精胺含量相对于野生型番茄明显提高,转基因植株生长发育迟缓、开花期延迟,外源施用赤霉素能有效恢复转基因植株生长势,基因表达分析表明转基因植株部分赤霉素合成相关基因表达明显受到抑制。
4.应用染色体步移和电子克隆技术获得PpADC基因启动子,其全长为2043bp。生物信息学分析表明启动子的转录起始位点为A碱基,位于翻译起始位点ATG上游-453bp处,其序列含有典型的TATA box,并且含有干旱、低温、病原菌等逆境胁迫响应元件,以及生长素、伤害和生物钟调控响应元件。通过构建启动子全长和5’系列缺失的GUS植物表达载体,遗传转化Micro-Tom番茄,GUS组织化学染色分析表明PpADC启动子具有正常的启动子活性。
5.PtADC转基因功能分析结果表明,转基因番茄中PtADC基因超量表达,转基因植株中内源腐胺和亚精胺含量相对于野生型番茄明显提高,但内源精胺含量没有明显变化。离体叶片脱水处理过程中转基因植株的失水率明显低于野生型番茄。处理结束时,形态学观察表明野生型番茄离体叶片相对于转基因植株明显萎蔫,转基因植株离体叶片的相对电导率明显低于野生型番茄,而叶绿素含量与相对电导率表现相反结果,并且NBT和DAB染色结果表明转基因植株ROS含量明显低于野生型番茄。干旱处理导致野生型番茄萎蔫程度明显高于转基因植株。
Peach is one of the most important economic fruit trees among the world. The release and promotion of new cultivar underpin the healthy and sustainable development of peach industry. Genetic engineering is a feasible approach for cultivar breeding because of the sound technique foundation and high efficiency. But discovery of the desirable genes should be the prerequisite. Peach enjoys the unique characteristics of ripening and the accompanying biochemical mechanism has been studied intensively. Therefore, it has become the model for ripening study in fruit tree. In addition, peach is of no exception concerning stress restriction, which has provoked breeders throughout the world to create new cultivars with tolerance to multiple stresses in breeding program. In the current study, PpERS1and PpADC genes along with their respective promoters were cloned from Prunus persica and transferred into Micro-Tom tomato for functional analysis. Meanwhile, the transgenic tomato carrying PtADC were used to investigate its function under dehydration and drought stress. Main results were as follows:
1. Based on the homogenous sequences, an ethylene receptor gene PpERSl was cloned from peach, containing an open reading frame (ORF) of1935bp which encodes a644amino acids polypeptide with a predicted molecular mass of72.4kDa and an isoelectric point of6.33. Analysis of the putative amino acid sequence suggests that PpERS1was clustered to into the ETR1subfamily, and showed high homology with those ethylene receptors from rosaceous plants. Bioinformatics analysis revealed that the coding region of PpERS1contained five exons interrupted by four introns, in addition, two introns located in the5'UTR of PpERSl gene, and alternative splicing occurred in the first intron of5'UTR. The expression analysis indicated that the PpERSl transcript was detected in all the tissues including root, stem, leaf, flower, fruitlet and ripening fruit. The expression of PpERSl was lowest in root and the expressions in stem, leaf, flower, fruitlet and ripening fruit were1.5,1.8,2.4,1.5and4.3fold higher to that of root, respectively. PpERSl expression was induced by injury, dehydration, ethephon, salt and peach tree gummosis, while low temperature down-regulated PpERSl transcription.
2. A2798bp upstream region of PpERSl translation start codon (ATG) was cloned by genomic walking PCR.5'RACE result revealed that the transcription start site was G, located at-777bp upstream of ATG of PpERS1. In silico analysis of the PpERS1promoter revealed the presence of typical TATA box and CAAT box, ethylene response element, injury response element, enhancer element, hormone response elements including auxin, cytokinin, abscisic acid and gibberellin, and stress response elements including drought, low temperature and pathogen. We constructed vectors containing the PpERSl full-length and serial5'-deleted promoters with GUS reporter gene, and all the constructions were introduced into tomato (Lycopersicon esculentum cv. Micro-Tom) through Agrobacterium tumefaciens-mediated transformation. GUS activity analysis was carried out by histochemical staining and fluorescence detection demonstrated that the promoter activity was normal in transgenic plants and not affected by the introns contained in5'UTR. GUS activity analysis in tissue, organs and developmental stages indicated the PpERSl promoter was active in almost all tissues. Meanwhile, PpERS1promoter activity was detected in radicle, cotyledons, hypocotyls and euphylla during different development stages. Real Time PCR analysis suggested that promoter activity were induced by injury and ethephon treatment, suppressed by low temperature and immune to salt treatment.
3. Based on the homogenous sequences, an arginine decarboxylase gene PpADC was cloned from peach, containing an open reading frame (ORF) of2178bp which encodes a725amino acids polypeptide with a predicted molecular mass of77.7kDa and an isoelectric point of5.19. Analysis of the putative amino acid sequence suggests that PpADC shared high homology with that from rosaceous plants. Bioinformatics analysis revealed that no intron was contained in the coding region of PpADC. The expression analysis indicated that the PpADC transcription were up-regulated by dehydration, ethephon, salt and low temperature treatment. The transgenic plants overexpressing PpADC contained higher Put, Spd and Spm level than that of the wild type (WT). Meanwhile, the transgenic plants showed dwarfism and late-flowering. However, the phenotype was rescued by gibberellin application. The expression analysis indicated that partial genes associated with gibberellin biosynthesis were suppressed in transgenic plant.
4. A2043bp upstream region of PpADC translation start codon (ATG) was cloned by genomic walking and silicon cloning PCR. In silico analysis of the PpADC promoter revealed that the transcription start site was A, located at-453bp upstream of ATG of PpADC. The sequence of PpADC promoter contained the putative TATA box, stress response elements including drought, low temperature and pathogen, auxin response element, injury response element, and circadian regulation element. Vectors containing the PpADC full-length and serial5'-deleted promoters with GUS reporter gene were constructed and introduced into tomato(Lycopersicon esculentum cv. Micro-Tom) through Agrobacterium tumefaciens-mediated transformation. GUS activity analysis carried out by histochemical staining demonstrated that the promoter activity was normal in transgenic plants.
5. The transgenic plants overexpressing PtADC contained higher Put and Spd level than the WT, while there was no difference in Spm level. Under dehydration, water loss of detached leaves was severer in the WT. Moreover, ion leakage of the transgenic lines was remarkably lower than that of the WT. However, chlorophyll content was higher in the WT. In addition, NBT and DAB histochemical staining revealed that WT accumulated more ROS than the transgenic lines in tomato. Therefore, drought tolerance of the transgenic lines was obviously improved.
引文
1.安丰英,郭红卫.乙烯信号转导的分子机制.植物学通报,2006,23：531-542.
2.柏亚男,吴茂森,何晨阳.水稻基因启动子OsBTF3p的克隆和启动活性分析.中国农业科学,2009,42：862-868.
3.黄培堂等译,Joseph S, David WR.分子克隆实验指南,第三版,北京：科学出版社,2002.
4.李建勇,卢钢,任彦.多胺在果实生长发育中的作用研究进展.果树学报,2005,3：256-260.
5.李敏,杨谦.一种高效构建同源重组DNA片段的方法——融合PCR.中国生物工程杂志,2007,27：53-58.
6.刘红霞.1-MCP, BTH和PHC对桃果(Prunus persica L.)采后衰老的调控作用及诱导抗病机理的研究.[博士学位论文].北京：中国农业大学,2004.
7.刘小花,张岚岚,朱长青,陈昆松,徐昌杰.Micro-Tom番茄矮化微型机制及其在植物功能基因组学研究中的应用.遗传,2008,30：1257-1264.
8.田世平主编.果蔬产品采后贮藏加工与包装技术指南.北京：中国农业出版社,2000,100.
9.汪祖华,庄恩及主编.中国果树志—桃卷.北京：中国林业出版社,2001,1-16.
10.王关林,方宏筠主编.植物基因工程.北京：科学出版社,2002.
11.王静.柑橘类植物非生物胁迫下多胺变化及两个多胺生物合成基因的克隆与鉴定.[博士学位论文].武汉：华中农业大学,2009.
12.吴延军.桃组织培养及遗传转化的研究.[博士学位论文].杭州：浙江大学,2004,13-19.
13. Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. The Plant Cell,2003,15:63-78.
14. Abeles FB, Morgan PW, Saltveit ME. Ethylene in plant biology.2nd ed. (San Diego, CA:Academic Press),1992.
15. Agarwal M, Hao Y, Kappoor A, Dong CH, Fujii H, Zheng X, Zhu JK. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. The Journal of Biological Chemistry,2006, 281:37636-37645.
16. Akiyama T, Jin S. Molecular cloning and characterization of an arginine decarboxylase gene up-regulated by chilling stress in rice seeding. Journal of Plant Physiology,2007,164:645-654.
17. Alcazar R, Altabella T, Marco F, Bortolotti C, Reymond M, Koncz C, Carrasco P, Tiburcio AF. Polyamines:molecules with regulatory functions in plant abiotic stress tolerance. Planta,2010,231:1237-1249.
18. Alcazar R, Cuevas JC, Planas J, Zarza X, Bortolotti C, Carrasco P, Salina J, Tiburcio AF, Altabella T, Integration of polyamines in the cold acclimation response, Plant Science,2011,180:31-38.
19. Alcazar R, Garcia-Martinez JL, Cuevas JC, Tiburcio AF, Altabella T. Overexpression of ADC2 in Arabidopsis induces dwarfism and late-flowering through GA deficiency. The Plant Journal,2005,43:425-436.
20. Alcazar R, Marco F, Cuevas JC, Patron M, Ferrando A, Carrasco P, Tiburcio AF, Altabella T. Involvement of polyamines in plant response to abiotic stress. Biotechnology Letters,2006,28:1867-1876.
21. Alonso JM, Hirayama T, Roman G, Nourizadeh S, Ecker JR. EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science,1999, 284:2148-2152.
22. An SH, Choi HW, Hong JK, Hwang BK. Regulation and function of the pepper pectin methylesterase inhibitor (CaPMEI1) gene promoter in defense and ethylene and methyl jasmonate signaling in plants. Planta,2009,230:1223-1237.
23. Anderson JP, Singh KB. Interactions of Arabidopsis and M. truncatula with the same pathogens differ in dependence on ethylene and ethylene response factors. Plant Signaling & Behavior,2011,6:551-552.
24. Argueso CT, Hansen M, Kieber JJ. Regulation of ethylene biosynthesis. Journal of Plant Growth Regulation,2007,26:92-105.
25. Arigita L, Tames RS, Gonzalez A. Ethylene biosynthesis and endogenous polyamines in relation to development of in vitro cultured kiwifruit explants. Functional Plant Biology,2004,31,603-609.
26. Arrom L, Munne-Bosch S. Hormonal changes during flower development in floral tissues of Lilium. Planta,2012, DOI:10.1007/s00425-012-1615-0.
27. Aziz A. Spermidine and related-metabolic inhibitors modulate sugar and amino acid levels in Vitis vinifera L.:possible relationships with initial fruitlet abscission. Journal of Experimental Botany,2003,54:355-363.
28. Bailey TA, Zhou X, Chen J, Yang Y. Role of ethylene, abscisic acid and MAP kinase pathways in rice blast resistance. Advances in Genetics, Genomics and Control of Rice Blast Disease,2009, Part Ⅱ,185-190.
29. Baninasab B, Rahemi M. Effect of exogenous polyamines on flower bud retention in pistachio (Pistacia vera L.) trees. Horticulture, Environment and Biotechnology, 2008,49:149-154.
30. Baron K, Stasolla C. The role of polyamines during in vivo and in vitro development. In vitro Cellular & Developmental Biology-Plant,2008,44:384-395.
31. Barry CS, Liop-Tous MI, Grierson D. The regulation of 1-aminocyclopropane-l-carboxylic acid synthase gene expression during the transition from system-1 to system-2 ethylene synthesis in tomato. Plant Physiology, 2000,123:979-986.
32. Bassett CL, Artlip TS, Callahan AM. Characterization of the peach homologue of the ethylene receptor, PpETR1, reveals some unusual features regarding transcript processing. Planta,2002,215:679-688.
33. Bell E, Malmberg RL. Analysis of a cDNA encoding arginine decarboxylase from oat reveals similarity to the Eschericbia coli arginine decarboxylase and evidence of protein processing. Molecular and General Genetics,1990,224:431-436.
34. Berrocal-Lobo M, Molina A. Ethylene response factor 1 mediates Arabidopsis resistance to the soilborne fungus Fusarium oxysporum. Molecular Plant-microbe Interactions,2004,17:763-770.
35. Bhatnagar-Mathur P, Vadez V, Sharma KK. Transgenic approaches for abiotic stress in plants:retrospect and prospects. Plant Cell and Report,2008,27:411-424.
36. Biasi R, Bagni N and Costa G. Endogenous polyamines in apple and their relationship to fruit set and fruit growth. Physiologia Plantarum,2006,73:201-205.
37. Binder BM. The ethylene receptors:complex perception for a simple gas. Plant Science,2008,175:8-17.
38. Bisson M, Bleckmann A, Allekotte S, Groth G. EIN2, the central regulator of ethylene signalling, is localised at the ER membrane where it interacts with the ethylene receptor ETR1. Biochemical Journal,2009,424,1:1-6.
39. Black DL, Graveley BR. Splicing bioinformatics to biology. Genome Biology,2006, 7:317.
40. Bleecker AB, Kende H. Ethylene:A gaseous signal molecule in plants. Annual Review of Cell and Developmental Biology,2000,16:1-18.
41. Bleecker AB. Ethylene perception and signaling:an evolutionary perspective. Trends in Plant Science.1999,4:269-274.
42. Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress:a review. Annals of Botany,2003,91:179-194.
43. Bortolotti C, Cordeiro A, Alcazar R, Borrell A, Culianez-macia FA, Tiburcio AF, Altabella T. Localization of arginine decarboxylase in tobacco plants. Physiologia Plantarum,2004,120:84-92.
44. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry,1976,72:248-254.
45. Brosche M, Overmyer K, Wrzaczek M, Kangajarvi J, Kangajarvi S. Stress signaling Ⅲ:Reactive Oxygen Species (ROS). Abiotic Stress Adaptation in Plants,2010, 1:91-102.
46. Cai M, Qiu DY, Yuan T, Ding XH, Li HJ, Duan L, Xu CG, Li XH, Wang SP. Identification of novel pathogen-responsive cis-elements and their binding proteins in the promoter of OsWRKYl3, a gene regulating rice disease resistance. Plant, Cell and Environment,2008,31:86-96.
47. Candan AP, Graell J, Larrigaudiere C. Roles of climacteric ethylene in the development of chilling injury in plums. Postharvest Biology and Technology,2008, 47:107-112.
48. Cao WH, Liu J, He XJ, Mu RL, Zhou HL, Chen SY, Zhang JS. Modulation of ethylene responses affects plant salt-stress responses. Plant Physiology,2007, 143:707-719.
49. Cao WH. Liu J. Zhou QY. Cao YR. Zheng SF. Du BX. Zhang JS. Chen SY. Expression of tobacco ethylene receptor NTHK1 alters plant responses to salt stress. Plant, Cell & Environment,2006,29:1210-1219.
50. Cao YR, Chen SY, Zhang JS. Ethylene signaling regulates salt stress response. Plant Signaling & Behavior,2008,3:761-763.
51. Capell T, Bassie L, Christou P. Modulation of the polyamine biosynthetic pathways in transgenic rice confers tolerance to drought stress. Proceeding of the National Academy of Sciences of the United States of America,2004,101:9909-9914.
52. Capell T, Escobar C, Liu H, Burtin D, Lepri O, Christou P. Over-expression of the oat arginine decarboxylase cDNA in transgenic rice (Oryza sativa L.) affects normal development patterns in vitro and results in putrescine accumulation in transgenic plants. Theoretical and Applied Genetics,1998,97:246-254. plants. Genes & Genomics,2009,31:107-118.
54. Carninci P, Sandelin A, Lenhard B, Katayama S, Shimokawa K, Ponjavic J, Semple CAM, Taylor MS, Engstrom PG, Frith MC, Forrest ARR, Alkema WB, Tan SL, Plessy C, Kodzius R, Ravasi T, Kasukawa T, Fukuda S, Kanamori-Katayama M, Kitazume Y, et al. Genome-wide analysis of mammalian promoter architecture and evolution. Nature Genetics,2006,38:626-635.
55. Chao Q, Rothenberg M, Solano R, Roman G, Terzaghi W, Ecker JR. Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins. Cell,1997,89:1133-1144.
56. Chen X, Wang Z, Gu R, Fu J, Wang J, Zhang Y, Wang M, Zhang J, Jia J, Wang G. Isolation of the maize Zpul gene promoter and its functional analysis in transgenic tobacco plants. Plant Cell Reports,2007,26:1555-1565.
57. Chen YF, Etheridge N, Schaller GE. Ethylene signal transduction. Annals of Botany, 2005,95:901-915.
58. Chen YF, Randlett MD, Findell JL, Schaller GE. Localization of the ethylene receptor ETR1 to the endoplasmic reticulum of Arabidopsis. The Journal of Biology Chemistry,2002,277:19861-19866.
59. Choudhury SR, Roy S, Das R, Sengupta DN. Differential transcriptional regulation of banana sucrose phosphate synthase gene in response to ethylene, auxin, wounding, low temperature and different photoperiods during fruit ripening and functional analysis of banana SPS gene promoter. Planta,2008,229:207-223.
60. Chuck G. Molecular mechanisms of sex determination in monoecious and dioecious plants. Advances in Botanical Research,2010,54:53-83.
61. Chung BYW, Simons C, Firth AE, Brown CM, Hellens RP. Effect of 5'UTR introns on gene expression in Arabidopsis thaliana. BMC Genomics,2006,7:20.
62. Ciardi J, Klee H, Regulation of ethylene-mediated responses at the level of the receptor, Annals of Botany,2001,88:813-822.
63. Ciardi JA, Tieman DM, Lund ST, Jones JB, Stall RE, Klee HJ. Response to Xanthomonas campestris pv. Vesicatoria in tomato involves regulation of ethylene receptor gene expression. Plant Physiology,2000,123:81-92.
64. Clancy M, Hannah LC. Splicing of the maize Sh1 first intron is essential for enhancement of gene expression, and a T-Rich motif increases expression without affecting splicing. Plant Physiology,2002,130:918-929.
65. Couee I, Hummel I, Sulmon C, Gouesbet G and Amrani AE. Involvement of polyamines in root development. Plant Cell, Tissue and Organ Culture,2004,76: 1-10.
66. Dhadi SR, Krom N, Ramakrishna W. Genome-wide comparative analysis of putative bidirectional promoters from rice, Arabidopsis and Populus. Gene,2009,429:65-73.
67. Dong CH, Rivarola M, Resnick JS, Maggin BD, Chang C. Subcellular co-localization of Arabidopsis RTE1 and ETR1 supports a regulatory role for RTE1 in ETR1 ethylene signalling. The Plant Journal,2008,53:275-286.
68. Doornbos RF, Geraats BP, Kuramae EE, van Loon LC, Bakker P. Effects of jasmonic acid, ethylene, and salicylic acid signaling on the rhizosphere bacterial community of Arabidopsis thaliana. Molecular Plant-Microbe Interactions,2011,24:395-407
69. Eapelund M, Jakobsen KS. Cloning and direct sequencing of plant promoters using primer-adaptor mediated PCR on DNA coupled to a magnetic solid phase. Biotechniques,1992,13:74-81.
70. Ecker JR, Davis RW. Plant defense genes are regulated by ethylene. Proceeding of the National Academy of Sciences of the United States of America,1987,84: 5202-5206.
71. Farooq M, Wahid A, Lee DJ. Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status, photosynthesis and membrane properties. Acta Physiologiae Plantarum,2009,31:937-945.
72. Filichkin SA, Priest HD, Givan SA, Shen R, Bryant DW, Fox SE, Wong WK, Mockler TC. Genome-wide mapping of alternative splicing in Arabidopsis thaliana. Genome Research,2010,20:45-58.
73. Fraga MF, Berdasco M, Diego LB, Rodriguez R, Canal MJ. Changes in polyamine concentration associated with aging in Pinus radiata and Prunus persica. Tree Physiology,2004,24:1221-1226.
74. Gamalero E, Glick BR. Ethylene and abiotic stress tolerance in plants. Environmental Adaptations and Stress Tolerance of Plants in the Era of Climate Change,2012,395-412.
75. Gao Z, Chen YF, Randlett MD, Zhao XC, Findell JL, Kieber JJ, Schaller GE. Localization of the Raf-like kinase CTR1 to the endoplasmic reticulum of Arabidopsis through participation in ethylene receptor signaling complexes. The Journal of Biological Chemistry,2003,278:34725-34732.
76. Ge C, Cui X, Wang Y, Hu Y, Fu Z, Zhang D, Cheng Z, Li J. BUD2, encoding an S-adenosylmethionine decarboxylase, is required for Arabidopsis growth and development. Cell Research,2006,16:446-456.
77. Gemperlova L, Eder J, Cvikrova M. Polyamine metabolism during the growth cycle of tobacco BY-2 cells. Plant Physiology and Biochemistry,2005,43:375-381.
78. Gill SS, Tuteja N. Polyamines and abiotic stress tolerance in plants. Plant Signaling & Behavior,2010,5:26-33.
79. Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry,2010, 48:909-930.
80. Giovannoni J. Molecular biology of fruit maturation and ripening. Annual Review of Plant Physiology and Plant Molecular Biology,2001,52:725-749.
81. Gomez MD, Beltran JP, and Canas LA. The pea END] promoter drives anther-specific gene expression in different plant species. Planta,2004,219: 967-981.
82. Gowik U, Burscheidt J. Akyildiz M., Schlue U., Koczor M., Streubel M., and Westhotf P. cis-regulatory elements for mesophyll-specific gene expression in the C4 plant Flaveria trinervia, the promoter of the C4 phosphoenolpyruvate carboxylase gene. Plant Cell,2004,16:1077-1090.
83. Grefen C, Stadele K, Ruzicka K, Obrdlik P, Harter K, Horak J. Subcellular localization and in vivo interactions of the arabidopsis thaliana ethylene receptor family members. Molecular Plant,2008,1:308-320.
84. Groppa MD, Benavides MP. Polyamines and abiotic stress:recent advances. Amino Acids,2008,34:35-45.
85. Guo DP, Sun YZ, Chen ZJ. Involvement of polyamines in cytoplasmic male sterility of stem mustard (Brassica juncea var. tsatsai). Plant Growth Regulation,2003, 41:33-40.
86. Gutterson N, Reuber TL. Regulation of disease resistance pathways by AP2/ERF transcription factors. Current Opinion in Plant Biology,2004,7:465-471.
87. Hamilton DA, Schwarz YH, Mascarenhas JP. A monocot pollen-specific promoter contains separable pollen-specific and quantitative elements. Plant Molecular Biology,1998,38:663-669.
88. Hanfrey C, Sommer S, Mayer MJ, Burtin D, Michael AJ. Arabidopsis polyamine biosynthesis:absence of ornithine decarboxylase and the mechanism of arginine decarboxylase activity. Plant Journal,2001,27:551-560.
89. Hao YJ, Kitashiba H, Honda C, Nada K, Moriguchi T. Expression of arginine deccarboxylase and ornithine decarboxylase genes in apple cells and stressed shoots. Journal of Experimental Botany,2005,56:1105-1115.
90. He LX, Ban Y, Inoue H, Matsuda N, Liu J, Moriguchi T. Enhancement of spermidine content and antioxidant capacity in transgenci pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis. Phytochemistry,2008, 69:2133-2141.
91. Higo K, Ugawa Y, Iwamoto M, Korenaga T. Plant cis-acting regulatory DNA elements (PLACE) database:1999. Nucleic Acids Research,1999,27:297-300.
92. Hirano K, Ueguchi-Tanaka M, Matsuoka M. GID1-mediated gibberellin signaling in plants. Trends in Plant Science,2008,13:192-199.
93. Hoth S, Niedermeier M, Feuerstein A, Hornig J, Sauer N. An ABA-responsive element in the AtSUC1 promoter is involved in the regulation of AtSUC1 expression. Planta,2010,232:911-923.
94. Hua J, Chang C, Sun Q, Meyerowitz EM. Ethylene insensitivity conferred by Arabidopsis ERS gene. Science,1995,269:1712-1714.
95. Hua J, Meyerowitz EM. Ethylene responses are negatively regulated by a receptor gene family in Arabidopsis thaliana. Cell,1998,94:261-271.
96. Huang CK, Chang BS, Wang KC, Her SJ, Chen TW, Chen YA, Cho CL, Liao LJ, Huang KL, Chen WS, Liu ZH. Changes in polyamine pattern are involved in floral initiation and development in Pelianthes tuberosa. Journal of Plant Physiology,2004, 161:709-713.
97. Huang XS, Liu JH, Chen XJ. Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes. BMC Plant Biology,2010,10:230, doi:10.1186/1471-2229-10-230.
98. Huang XS, Luo T, Fu XZ, Fan QJ, Liu JH. Cloning and molecular characterization of a mitogen-activated protein kinase gene from Poncirus trifoliata whose ectopic expression confers dehydration/drought tolerance in transgenic tobacco. Journal of Experimental Botany,2011,62:5191-5206.
99. Huang Y, Li H, Hutchison CE, Laskey J, Kieber JJ. Biochemical and functional analysis of CTR1, a protein kinase that negatively regulates ethylene signaling in Arabidopsis. Plant Journal,2003,33:221-233.
100.Hummel I, Bourdais G, Gouesbet G, Couee I, Malmberg RL, Amrani AE. Differential gene expression of ARGININE DECARBOXYLASE ADC1 and ADC2 in Arabidopsis thaliana:characterization of transcriptional regulation during seed germination and seedling development. New Phytologist,2004,163:519-531.
101.Hussain SS, Ali M, Ahmad M, Siddique KHM. Polyamines:Natural and engineered abiotic and biotic stress tolerance in plants. Biotechnology Advances,2011,29: 300-311.
102.Imai A, Matsuyama T, Hanzawa Y, Akiyama T, Tamaoki M, Saji H, Shirano Y, Kato T, Hayashi H, Shibata D, Tabata S, Komeda Y, Takahashi T. Spermidine synthase genes are essential for survival of Arabidopsis. Plant Physiology,2004, 135:1565-1573.
103.Ish-Shalom M, Dahan Y, Maayan I, Irihimovitch V. Cloning and molecular characterization of an ethylene receptor gene, MiERS1, expressed during mango fruitlet abscission and fruit ripening. Plant Physiology and Biochemistry,2011, 49:931-936.
104.Ivanchenko MG, Muday GK, Dubrovsky JG. Ethylene-auxin interactions regulate lateral root initiation and emergence in Arabidopsis thaliana. The Plant Journal,2008, 55:335-347.
105.Iwai T, Miyasaka A, Seo S, Ohashi Y. Contribution of ethylene biosynthesis for resistance to blast fungus infection in young rice plants. Plant Physiology,2006, 142:1202-1215.
106.Johnson PR, Ecker JR. The ethylene gas signal transduction pathway:A molecular perspective. Annual Review of Genetics,1998,32:227-254.
107.Juven-Gershon T, Hsu JY, Theisen JWM, Kadonaga JT. The RNA polymerase Ⅱ core promoter-the gateway to transcription. Current Opinion in Cell Biology,2008, 20:253-259.
108.Kakkar RK, Sawhney VK. Polyamine research in plants-a changing perspective. Physiologia Plantarum,2002,116:281-292.
109.Karthikeyan AS, Ballachanda DN, Raghothama KG. Promoter deletion analysis elucidates the role of cis elements and 5'UTR intron in spatiotemporal regulation of AtPht1;4 expression in Arabidopsis. Physiologia Plantarum,2009,136:10-18.
110.Katerova ZI, Todorova D. Endogenous polyamines lessen membrane damages in pea plants provoked by enhanced ultraviolet-C radiation. Plant Growth Regulation,2009, 57:145-152.
111.Kaur-Sawhney R, Tiburcio AF, Altabella T, Galston AW. Polyamines in plants:A overview. Journal of Cell and Molecular Biology,2003,2:1-12.
112.Kazmierczak A. Giberellic acid and ethylene control male sex determination and development of anemia phyllitidis gametophytes. Working With Ferns,2010, Part I 49-65.
113.Kendrick MD, Chang C. Ethylene signaling:new levels of complexity and regulation. Current Opinion in Plant Biology,2008,11:479-485.
114.Kepczynski J, Kepczynska E. Manipulation of ethylene biosynthesis. Acta Physiologiae Plantarum,2005,27:213-220.
115.Kieber JJ, Rothenberg M, Roman G, Feldman KA, Ecker JR. CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the Raf family of protein kinases. Cell,1993,72:427-441.
116.Kizis D, Lumbreras V, Pages M.Role of AP2/EREBP transcription factors in gene regulation during abiotic stress. FEBS Letters,2001,498:187-189.
117.Kizis D, Pages M. Maize DRE-binding proteins DBF1 and DBF2 are involved in rabl7 regulation through the drought-responsive element in an ABA-dependent pathway. The Plant Journal,2002,30:679-689.
118.Klee HJ, Tieman D. The tomato ethylene receptor family:Form and function. Physiologia Plantarum,2002,115:336-341.
119.Kobayashi T., Nakayama Y., Itai R.N., Nakanishi H., Yoshihara T., Mori S., and Nishizawa N.K. Identification of novel cis-acting elements, IDE1 and IDE2, of the barley IDS2 gene promoter conferring iron-deficiency-inducible, root-specific expression in heterogeneous tobacco plants. Plant Journal,2003,36:780-793.
120.Kropat J, Tottey S, Birkenbihl RP, Depege N, Huijser P, Merchant S. A regulator of nutritional copper signaling in chlamydomonas is an SBP domain protein that recognizes the GTAC core of copper response element. Proceeding of the National Academy of Sciences of the United States of America,2005,102:18730-18735.
121.Kunkel BN, Brooks DM. Cross talk between signaling pathways in pathogen defense. Current Opinion in Plant Biology,2002,5:325-331.
122.Kunkel BN, Brooks DM. Cross talk between signaling pathways in pathogen defense. Current Opinion in Plant Biology,2002,5:325-331.
123.Kusano T, Berberich T, Tateda C, Takahashi Y. Polyamines:essential factors for growth and survival. Planta,2008,228:367-381.
124.Laquitaine L, Gomes E, Francois J, Marchive C, Pascal S, Hamdi S, Atanassova R, Delrot S, Coutos-Thevenot P. Molecular basis of ergosterol-induced protection of grape against botrytis cinerea:induction of type I LTP promoter activity, WRKY, and stilbene synthase gene expression. Molecular Plant-Microbe Interactions.2006, 19:1103-1112.
125.Leclercq J, Adams-Phillips LC, Zegzouti H, Jones B, Latche A, Giovannonni JJ, Pech JC, Bouzayen M. LeCTR1, a tomato CTR1-like gene, demonstrates ethylene signaling ability in Arabidopsis and novel expression patterns in tomato. Plant Physiology,2002,130:1132-1142.
126.Lee SC, Kim DS, Kim NH, Hwang BK. Functional analysis of the promoter of the pepper pathogen-induced gene, CAPIP2, during bacterial infection and abiotic stresses. Plant Science,2007,172:236-245.
127.Lelievre JM, Latche A, Jones B, Bouzayen M, Pech JC. Ethylene and fruit ripening. Physiologia Plantarum,1997,101:727-739.
128.Leon-Reyes A, Du Y, Koornneef A, Proietti S, Korbes AP, Memelink J, Pieterse C, Ritsema T. Ethylene signaling renders the jasmonate response of Arabidopsis insensitive to future suppression by salicylic acid. Molecular Plant-Microbe Interactions,2010,23:187-197.
129.Lescot M, Dehais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouze P, Rombauts S. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Research, 2002,30:325-327.
130.Lewis MW, Leslie ME, Liljegren SJ. Plant separation:50 ways to leave your mother. Current Opinion in Plant Biology,2008,9:59-65.
131.Li CZ, Jiao J, Wang GX. The important roles of reactive oxygen species in the relationship between ethylene and polyamines in leaves of spring wheat seedlings under root osmotic stress. Plant Science,2004,166:303-315.
132.Li Y, Wu Z, Ma N, Gao J. Regulation of the rose RH-PIP2;1 promoter by hormones and abiotic stresses in Arabidopsis. Plant Cell Reports,2009,28:185-196.
133.Liang Y, Bae H, Kang S, Lee T, Kim M, Kim Y, Ha S. The Arabidopsis beta-carotene hydroxylase gene promoter for a strong constitutive expression of transgene. Plant Biotechnology Reports,2009,3:325-331.
134.Lim PO, Kim HJ, Nam HG. Leaf senescence. Annual Review of Plant Biology,2007, 58:115-136.
135.Lin Z, Zhong S, Grierson D. Recent advances in ethylene research. Journal of Experimental Botany,2009,60:3311-3336.
136.Linkies A, Leubner-Metzger G. Beyond gibberellins and abscisic acid:how ethylene and jasmonates control seed germination. Plant Cell Reports,2012,31:253-270.
137.Liu H, Creech RG, Jenkins JN, Ma D. Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3. Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids,2000,1487:106-111.
138.Liu JH, Ban Y, Wen XP, Nakajima I, Moriguchi T. Molecular cloning and expression analysis of an arginine decarboxylase gene from peach(Prunus persica). Gene,2009, 429:10-17.
139.Liu JH, Honda C, Moriguchi T. Involvement of polyamine in floral and fruit development. Japan agricultural research quarterly,2006a,40:51-58.
140.Liu JH, Inoue H, Moriguchi T. Salt stress-mediated changes in free polyamine titers and expression of genes responsible for polyamine biosynthesis of apple in vitro shoots. Environmental and Experimental Batany,2008,62:28-35.
141.Liu JH, Kitashiba H, Wang J, Ban Y, Moriguchi T. Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnology,2007, 24:117-126.
142.Liu JH, Moriguchi T. Changes in free polyamines and gene expression during peach flower development. Biologia Plantarum,2007,51:530-532.
143.Liu JH, Nada K, Honda C, Kitashiba H, Wen XP, Pang XM, Moriguchi T. Polyamine biosynthesis of apple callus under salt stress:involvement of ADC pathway in stress response. Journal of Experimental Botany,2006c,57:2589-2599.
144.Liu JH, Nada K, Pang XM, Honda C, Kitashiba H, Moriguchi T. Role of polyamines in peach fruit development and storage. Tree Physiology,2006b,26:791-798.
145.Liu JH, Nakajima I, Moriguchi T. Effects of salt and osmotic stresses on free polyamine content and expression of polyamine biosynthetic genes in Vitis vinifera. Biologia Plantarum,2011,55:340-344.
146.Liu K, Fu HH, Bei QX, Luan S. Inward potassium channel in guard cells as a target for polyamine regulation of stomatal movements. Plant Physiology,2000, 124:1315-1325.
147.Lorenzo O, Piqueras R, Sanchez-Serrano JJ, Solano R. ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell,2003,15:165-178.
148.Lu J, Sivamani E, Azhakanandam K, Samadder P, Li X, Qu R. Gene expression enhancement mediated by the 5'UTR intron of the rice rubi3 gene varied remarkably among tissues in transgenic rice plants. Molecular Genetics and Genomics,2008, 279:563-572.
149.Ludwig AA, Saitoh H, Felix G, Freymark G, Miersch O, Wasternack C, Boller T, Jones JD, Romeis T. Ethylene-mediated cross-talk between calcium-dependent protein kinase and MAPK signaling controls stress responses in plants. Proceeding of the National Academy of Sciences of the United States of America,2005, 102:10736-10741.
150.Lurie S, Crisosto CH. Chilling injury in peach and nectarine. Postharvest Biology and Technology,2005,37:195-208.
151.Ma B, Cui ML, Sun HJ, Takada K, Mori H, Kamada H, Ezura H. Subcellular localization and membrane topology of the melon ethylene receptor CmERS1. Plant Physiology,2006,141:587-597.
152.ManaVella PA, Dezar CA, Ariel FD, Chan RL. Two ABREs, two redundant root-specific and one W-box cis-acting elements are functional in the sunflower HAHB4 promoter. Plant Physiology Biochemistry,2008,46:860-867.
153.Mao X, Zhang H, Tian S, Chang X, Jing R. TaSnRK2.4, an SNFl-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis. Journal of Experimental Botany,2010, 61:683-696.
154.Mascarenhas D, Mettler IJ, Pierce DA, Lowe HW. Intron-mediated enhancement of heterologous gene expression in maize. Plant Molecular Biology,1990,15:913-920.
155.Masgrau C, Altabella T, Farras R, Flores D, Thompson AJ, Besford RT, Tiburcio AF. Inducible overexpression of oat arginine decarboxylase in transgenic tobacco plants. The Plant Journal,1997,11:465-473.
156.Masura SS, Parveez GKA, Ti LLE. Isolation and characterization of an oil palm constitutive promoter derived from a translationally control tumor protein (TCTP) gene. Plant Physiology and Biochemistry,2011,49:701-708.
157.McManus MT, Harpaz-Saad S, Yoon GM, Mattoo AK, Kieber JJ. The formation of ACC and competition between polyamines and ethylene for SAM. Annual Plant Reviews,2012,44:DOI:10.1002/9781118223086.ch3.
158.Mehta RA, Cassol T, Li N, Ali N, Handa AK, Mattoo AK. Engineered polyamine accumulation in tomato enchances phytonutrient content, juice quality, and vine life. Nature Biotechnology,2002,20:613-618.
159.Meisel L, Fonseca B, Gonzalez S, Baezayates R, Cambiazo V, Campos R, Gonzalez M, Orellana A, Retamales J, Silva H. A rapid and efficient method for purifying high quality total RNA from peaches(Prunus persica) for functional genomics analyses. Biological Research,2005,38:83-88.
160.Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R. Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant, Cell & Environment,2010, 33:453-467.
161.Minocha R, Minocha SC, Long S. Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.). In Vitro Cellular & Developmental Biology-Plant,2004,40:572-580.
162.Mirdehghan SH, Rahemi M, Martinez-Romero D, Guillen F, Valverde JM, Zapata PJ, Serrano M, Valero D. Reduction of pomegranate chilling injury during storage after heat treatment:role of polyamines. Postharvest Biology and Technology,2007, 44:19-25.
163.Mo H, Pua EC. Up-regulation of arginine decarboxylase gene expression and accumulation of polyamines in mustard (Brassica juncea) in response to stress. Physiologia Plantarum,2002,114:439-449.
164.Moon H, Callahan AM. Developmental regulation of peach ACC oxidase promoter-GUS fusions in transgenic tomato fruits. Journal of Experimental Botany, 2004,55:1519-1528.
165.Morgan PW, Drew MC. Ethylene and plant responses to stress. Physiologia Plantarum,1997,100:620-630.
166.Nam KH, Lee SH, Lee J. Differential expression of ADC mRNA during development and upon acid stress in Soybean (Glycine mas) Hypocotyls. Plant Cell Physiology,1997,38:1156-1166.
167.Nambeesan S, Qamar SA, Laluk K, Mattoo AK, Mickelbart MV, Ferruzzi MG, Mengiste T, Handa AK. Polyamines Attenuate Ethylene-Mediated Defense Responses to Abrogate Resistance to Botrytis cinerea in Tomato. Plant Physiology, 2012,158:1034-1045.
168.Nayyar H, Chander S. Protective effects of polyamines against oxidative stress induced by water and cold stress in chickpea. Journal of Agronomy and Crop Science,2004,190:355-365.
169.Negi S, Ivanchenko MG, Muday GK. Ethylene regulates lateral root formation and auxin transport in Arabidopsis thaliana. The Plant Journal,2008,55:175-187.
170.Nishiuchi T, Shinshi H, Suzuki K. Rapid and transient activation of transcription of the ERF3 gene by wounding in tobacco leaves-Possible involvement of NtWRKYs and autorepression. Journal of Biological Chemistry,2004,279:55355-55361.
171.Nolke G, Schneider B, Fischer R, Schillberg S. Immunomodulation of polyamine biosynthesis in tobacco plants has a significant impact on polyamine levels and generates a dwarf phenotype. Plant Biotechnology Journal,2005,3:237-247.
172.O'Malley RC, Rodriguez FI, Esch JJ, Binder BM, O'Donnell P, Klee HJ, Bleecker AB. Ethylene-binding activity, gene expression levels, and receptor system output for ethylene receptor family members from Arabidopsis and tomato. The Plant Journal, 2005,41:651-65.
173.Pandey S, Ranade SA, Nagar PK, Kumar N. Role of polyamines and ethylene as modulators of plant senescence. Journal of Biosciences,2000,25,291-299.
174.Pang JH, Ma B, Sun HJ, Ortiz GI, Imanishi S, Sugaya S, Gemma H, Ezura H. Identification and characterization of ethylene receptor homologs expressed during fruit development and ripening in persimmon(Diospyros kaki Thumb.), Postharvest Biology and Technology,2007,44:195-203.
175.Pang XM, Zhang ZY, Wen XP, Ban Y, Moriguchi T. Polyamines, All-purpose players in response to environment stresses in plants. Plant Stress,2007,1:173-188.
176.Paschalidis KA, Roubelakis-Angelakis KA. Spatial and temporal distribution of polyamine levels and polyamine anabolism in different organs/tissues of the tobacco plant. Correlations with age, cell division/expansion, and differentiation. Plant Physiology,2005,138:142-152.
177.Paschalidis KA, Roubelakis-Angelakis KA. Spatial and temporal distribution of polyamine levels and polyamine anabolism in different organs/tissues of the tobacco plant. Correlations with age, cell division/expansion, and differentiation. Plant Physiology,2005,138:142-152.
178.Paul V, Pandey R, Srivastava GC. Tomato fruit ripening:regulation of ethylene production and its response. Indian Journal of Plant Physiology,2011,16:117-131
179.Pech JC, Purgatto E, Bouzayen M and Latche A. Ethylene and Fruit Ripening. Annual Plant Reviews,2012, Volume 44:The Plant Hormone Ethylene (doi:10.1002/9781118223086.chl1).
180.Peleg Z, Blumwald E. Hormone balance and abiotic stress tolerance in crop plants. Current Opinion in Plant Biology,2011,14:290-295.
181.Perez-Amador MA, Leon J, Green PJ, Carbonell J. Induction of the arginine decarboxylase ADC2 gene provides evidence for the involvement of polyamines in the wound response in arabidopsis. Plant Physiology,2002,130:1454-1463.
182.Poresbski S, Bailey GL, Baum RB. Modification of CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter,1997,12:8-15.
183.Potenza C, Aleman L, Sengupta-Gopalan C. Targeting transgene expression in research, agricultural, and environmental applications:Promoters used in plant transformation. In Vitro Cellular and Development Biology Plant,2004,40:1-22.
184.Primikirios NI, Roubelakis-Angelakis KA. Cloning and expression of an arginine decarboxylase cDNA from Vitis vinifera L. cell-suspension cultures. Planta,1999, 208:574-582.
185.Quinet M, Ndayiragije A, Lefevre I, Lambillotte B, Dupont-Gillain CC, Lutts S. Putrescine differently influences the effect of salt stress on polyamine metabolism and ethylene synthesis in rice cultivars differing in salt resistance. Journal of Experimental Botany,2010,61:2719-2733.
186.Rajesh MK, Radha E, Karun A, Parthasarathy VA. Plant regeneration from embryo-derived callus of oil palm-the effect of exogenous polyamines. Plant Cell, Tissue and Organ Culture,2003,75:41-47.
187.Rakitin VY, Prudnikova ON, Rakitina TY, Karyagin VV, Vlasov PV, Novikova GV, Moshkov IE. Interaction between ethylene and ABA in the regulation of polyamine level in Arabidopsis thaliana during UV-B stress. Russian Journal of Plant Physiology,2009,56:147-153.
188.Rasori A, Bertolasi B, Furini A, Bonghi C, Tonutti P, Ramina A. Functional analysis of peach ACC oxidase promoters in transgenic tomato and in ripening peach fruit. Plant Science,2003,165:523-530.
189.Rasori A, Ruperti B, Bonghi C, Tonutti P, Ramina A. Characterization of two putative ethylene receptor genes expressed during peach fruit development and abscission. Journal of Experimental Botany,2002,53:2333-2339.
190.Rastogi R, Dulson J, Rothstein SJ. Cloning of tomato (Lycopersicon esculentum Mill.) arginine decarboxylase gene and its expression during fruit ripening. Plant Physiology,1993,103:829-834.
191.Rawat R, Xu ZF, Yao KM, Chye ML, Identification of cis-elements for ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase. Plant Molecular Biology,2005,57:629-643.
192.Rhee HJ, Kim EJ, Lee JK. Physiological polyamines:simple primordial stress molecules. Journal of Cellular and Molecular Medicine,2007,11:685-703.
193.Richards DE, King KE, Ait-ali T, Harberd NP. How gibberellin regulates plant growth and development:a molecular genetic analysis of gibberellin signaling. Annual Review of Plant Physiology and Plant Molecular Biology,2001,52:67-88.
194.Romero-Puertas MC, Rodriguez-Serrano M, Corpas FJ, Gomez M, Rio Del LA, Sandalio LM. Cadmium-induced subcellular accumulation of O2- and H2O2 in pea leaves. Plant Cell & Environment,2004,27:1122-1134.
195.Roy M, Wu R. Arginine decarboxylase transgene expression and analysis of environmental stress tolerance in transgenic rice. Plant Science,2001,160:869-875.
196.Rushton PJ, Reinstadler A, Lipka V, Lippok B, Somssich LE. Synthetic plant promoters containing defined regulatory elements provide novel insights into pathogen- and wound-induced signaling. Plant Cell,2002,14:749-762.
197.Sairam PK, Tyagi A. Physiology and molecular biology of salinity stress tolerance in plants. Current Science,2004,86:407-421.
198.Salazar M, Gonzalez E, Casaretto JA, Casacuberta JM, Ruiz-Lara S. The promoter of the TLC1.1 retrotransposon from Solanum chilense is activated by multiple stress-related signaling molecules. Plant Cell Reports,2007,26:1861-1868.
199.Samadder P, Sivamani E, Lu J, Li X, Qu R. Transcriptional and post-transcriptional enhancement of gene expression by the 5'UTR intron of rice rubi3 gene in transgenic rice cells. Molecular Genetics Genomics,2008,279:429-439.
200.Schaller GE, Kieber JJ.2002. Ethylene. In:The Arabidopsis book. Rockville, MD: American Society of Plant Biologists (Online: http://www.aspb.org/publications/arabidopsis/).
201.Schaller GE. Ethylene and the regulation of plant development. BMC Biology,2012, 10:9.
202.Seiler N, Raul F. Polyamines and apoptosis. Journal of Cellular and Molecular Medicine,2005,9:623-642.
203.Shahmuradov IA, Gammerman AJ, Hancock JM, Bramley PM, Solovyev VV. PlantProm:a database of plant promoter sequences. Nucleic Acids Research,2003, 31:114-117.
204.Shi J, Fu XZ, Peng T, Huang XS, Fan QJ, Liu JH. Spermine pretreatment confers dehydration tolerance of citrus in vitro plants via modulation of antioxidative capacity and stomatal response. Tree Physiology,2010,30:914-922.
205.Simpson SD, Nakashima K, Narusaka Y, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Two different novel cis-acting elements of erdl, a clpA homologous Arabidopsis gene function in induction by dehydration stress and dark-induced senescence. The Plant Journal,2003,33:259-270.
206.Singer SD, Hily JM, Cox KD. The sucrose synthase-1 promoter from Citrus sinensis directs expression of the β-glucuronidase reporter gene in phloem tissue and in response to wounding in transgenic plants. Planta,2011,234:623-637.
207.Stepanova AN, Alonso JM. Ethylene signaling and response:where different regulatory modules meet. Current Opinion in Plant Biology,2009,12:548-555.
208.Sun HJ, Uchii S, Watanabe S, Ezura H. A highly efficient transformation protocol for Micro-Tom, A model cultivar for tomato functional genomics. Plant Cell Physiology,2006,47:426-431.
209.Sunilkumar G, Mohr L A, Lopata-Finch E, Emani C, Rathore K. Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP. Plant Molecular Biology,2002,50:463-74.
210.Swarup R, Perry P, Hagenbeek D, Van Der Straeten D, Beemster GT, Sandberg G, Bhalerao R, Ljung K, Bennett MJ. Ethylene upregulates auxin biosynthesis in Arabidopsis seedlings to enhance inhibition of root cell elongation. The Plant Cell, 2007,19:2186-2196.
211.Swiatecka-Hagenbruch M, Liere K, Borner T. High diversity of plastidial promoters in Arabidopsis thaliana. Molecular Genetics Genomics,2007,277:725-734.
212.Tadolini B. Polyamine inhibition of lipoperoxidation. The influence of polyamines on iron oxidation in the presence of compounds mimicking phospholipid polar heads. Biochemical Journal,1988,249:33-36.
213.Takahashi T, Kakehi JI. Polyamines:ubiquitous polycations with unique roles in growth and stress responses. Annals of Botany,2010,105:1-6.
214.Tang CF, Liu YG, Zeng GM, Li X, Xu WH, Li CF, Yuan XZ. Effects of exogenous spermidine on antioxidant system responses of Typha latifolia L. under Cd2+stress. Journal of Integrative Plant Biology,2005,47:428-434.
215.Tang W, Newton RJ. Polyamines promote root elongation and growth by increasing root cell division in regenerated Virginiapine (Pinusvirgiana Mill.) plantlets. Plant Cell Reports,2005,24:581-589.
216.Tanguy JM. Metabolism and function of polyamines in plants:recent development (new approaches). Plant Growth Regulation,2001,34:135-148.
217.Tieman DM, Ciardi JA, Taylor MG, Klee HJ. Members of the tomato LeEIL (EIN3-like) gene family are functionally redundant and regulate ethylene responses throughout plant development. The Plant Journal,2001,26:47-58.
218.Tonutti P, Bonghi C, Ramina A. Fruit firmness and ethylene biosynthesis in three cultivars of peach (Prunus persical L. Batsch). Journal of Horticultural Science, 1996,71(1):141-147.
219.Toquin V, Grausem B, Geotfroy P, Legrand M. Structure of the tobacco caffeic acid O-methyltransferase (COMT) Ⅱ gene:identification of promoter sequences involved in gene inducibility by various stimuli. Plant Molecular Biology,2003,52:495-509.
220.Trainotti L, Bonghi C, Ziliotto F, Zanin D, Rasori A, Casadoro G, Ramina A, Tonutti P. The use of microarray μPEACH1.0 to investigate transcriptome changes during transition from pre-climacteric to climacteric phase in peach fruit. Plant Science, 2006,170:606-613.
221.Urano K, Hobo T, Shinozaki K. Arabidopsis ADC genes involved in polyamine biosynthesis are essential for seed development. Federation of European Biochemical Societies Letters,2005,579:1557-1564.
222.Urano K, Yoshiba Y, Nanjo T, Ito T, Yamaguchi-Shinozaki K, Shinozaki K. Arabidopsis stress-inducible gene for arginine decarboxylase AtADC2 is required for accumulation of putrescine in salt tolerance. Biochemical and Biophysical Research Communications,2004,313:369-375.
223.Valero D. The role of polyamines on fruit ripening and quality during storage:what is new. Acta Horticulturae Home,2009,884:199-205.
224.Venter. Synthetic promoters:genetic control through cis engineering. Trends in Plant Science,2007,12:118-124.
225.Vera-Sirera F, Minguet EG, Singh SK, Ljung K, Tuominen H, Blazquez MA, Carbonell J. Role of polyamines in plant vascular development. Plant Physiology and Biochemistry,2010,48:534-539.
226.Verma S, Mishra SN. Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system. Journal of Plant Physiology,2005, 162:669-677.
227.Vickers CE, Xue G, Gresshoff PM. A high-level endosperm-specific expression in an novel cis-acting element, ESP, contributes to oat globulin promoter. Plant Molecular Biology,2006,62:195-214.
228.Wang DH, Li F, Duan QH, Han T, Xu ZH, Bai SN. Ethylene perception is involved in female cucumber flower development. The Plant Journal,2010,61:862-872.
229.Wang J, Sun PP, Chen CL, Wang Y, Fu XZ, Liu JH. An arginine decarboxylase gene PtADC from Poncirus trifoliata confers abiotic stress tolerance and promotes primary root growth in Arabidopsis. Journal of Experimental Botany,2011, 62:2899-2914.
230.Wang KLC, Li H, Ecker JR. Ethylene biosynthesis and signaling networks. Plant Cell (Suppl),2002,14:S131-S151.
231.Wang P, Zhang B, Li X, Xu CJ, Yin XR, Shan LL, Ferguson I, Chen KS. Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit. Journal of Experimental Botany,2010,61:179-190.
232.Wang W, Esch JJ, Shiu SH, Agula H, Binder BM, Chang C, Patterson SE, Bleecker AB. Identification of important regions for ethylene binding and signaling in the transmembrane domain of the ETR1 ethylene receptor of Arabidopsis. The Plant Cell,2006,18:3429-3442.
233.Wang W, Hall AE, O'Malley R, Bleecker AB. Canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor from Arabidopsis is not required for signal transmission. Proceeding of the National Academy of Sciences of the United States of America,2003,100:352-357.
234.Watson MW, Malmberg RL. Regulation of Arabidopsis thaliana (L.) Heynh arginine decarboxylase by potassium deWciency stress. Plant Physiology,1996, 111:1077-1083.
235.Watson MW, Yu W, Galloway GL, Malmberg RL. Isolation and characterization of a second arginine decarboxylase cDNA from Arabidopsis (PGR97-114). Plant Physiology,1997,114:1569.
236.Wen XP, Ban Y, Inoue H, Matsuda N, Moriguchi T. Spermidine levels are implicated in heavy metal tolerance in a spermidine synthase overexpressing transgenic European pear by exerting antioxidant activities. Transgenic Research,2009, 19:91-103.
237.Wilkinson S, Davies WJ. Drought, ozone, ABA and ethylene:new insights from cell to plant to community. Plant, Cell & Environment,2010,33:510-525.
238.Wolukau JN, Zhang SL, Xu GH, Chen D. The effect of temperature, polyamines and polyamine synthesis inhibit or on in vitro pollen germination and pollen tube growth of Prunus mume. Scientia Horticulturae,2004,99:289-299.
239.Wu XF, Wang CL, Xie EB, Gao Y, Fan YL, Liu PQ, Zhao KJ. Molecular cloning and characterization of the promoter for the multiple stress-inducible gene BjCHI1 from Brassica juncea. Planta,2009,229:1231-1242.
240.Xie C, Zhang JS, Zhou HL, Li J, Zhang ZG, Wang DW, Chen SY. Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco. The Plant Journal,2003,33:385-393.
241.Xie F, Liu Q, and Wen CK. Receptor signal output mediated by the ETR1 N-terminus is primarily subfamily I receptors-dependent. Plant Physiology,2006, 142:492-508.
242.Xu X, Shi G, Ding C, Xu Y, Zhao J, Yang H, Pan Q. Regulation of exogenous spermidine on the reactive oxygen species level and polyamine metabolism in Alternanthera philoxeroides (Mart.) Griseb under copper stress. Plant Growth Regulation,2011,63:251-258.
243.Yamaguchi K, Takahashi Y, Berberich T, Imai A, Takahashi T, Michael AJ, Kusano T. A protective role for the polyamine spermine against drought stress in Arabidopsis. Biochemical and Biophysical Research Communications,2007,352:486-490.
244.Yamaguchi S.Gibberellin metabolism and its regulation. Annual Review of Plant Biology,2008,59:225-251.
245.Yang J, Zhang J, Liu K, Wang Z, Liu L. Involvement of polyamines in the drought resistance of rice. Journal of Experimental Botany,2007,58:1545-1555.
246.Yang SF, Hoffman NE. Ethylene biosynthesis and its regulation in higher plants. Annual Review of Cell and Developmental Biology,1984,35:155-189.
247.Yi N, Kim YS, Jeong MH, Oh SJ, Jeong JS, Park SH, Jung H, Choi YD, Kim JK. Functional analysis of six drought-inducible promoters in transgenic rice plants throughout all stages of plant growth. Planta,2010,232:743-754.
248.Yin XR, Chen KS, Allan AC, Wu RM, Zhang B, Lallu N, Ferguson IB, Ethylene-induced modulation of genes associated with the ethylene signaling pathway in ripening kiwifruit. Journal of Experimental Botany,2008,59:2097-2108.
249.Yu SM, Ko SS, Hong CY, Sun HJ, Hsing YI, Tong CG, Ho THD. Global functional analyses of rice promoters by genomics approaches. Plant Molecular Biology,2007, 65:417-425.
250.Yuan S, Dean JFD. Differential responses of the promoters from nearly identical paralogs of loblolly pine (Pinus taeda L.) ACC oxidase to biotic and abiotic stresses in transgenic Arabidopsis thaliana. Planta,2010,232:873-886.
251.Zacchini M, de Agazio M. Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment. Plant Physiology and Biochemistry,2004,42:445-450.
252.Zarei A, Korbes AP, Younessi P, Montiel G, Champion A, Memelink J. Two GCC boxes and AP2/ERF-domain transcription factor ORA59 in jasmonate/ethylene-mediated activation of the PDF 1.2 promoter in Arabidopsis. Plant Molecular Biology,2011,75:321-331.
253.Zhang JS, Xie C, Shen YG, Chen SY. A two-component gene (NTHK1) encoding a putative ethylene-receptor homolog is both developmentally and stress regulated in tobacco. Theoretical and Applied Genetics,2001,102:815-824.
254.Zhao FG, Qin P. Protective effect of exogenous polyamines on root tonoplast function against salt stress in barley seedlings. Plant Growth Regulation,2004, 42:97-103.
255.Zheng H, Lin S, Zhang Q, Lei Y, Zhang Z. Function analysis of 5'untranslated region of a TIR-NBS-encoding gene from triploid white polar. Molecular Genetics and Genomics,2009,282:381-394.
256.Zhong S, Lin Z, Grierson D. Tomato ethylene receptor interaction:visualization of NEVER-RIPE interaction with multiple CTRs at the endoplasmic reticulum. Journal of Experimental Botany,2008,59:965-972.
257.Zhou HW, Dong L, Arie RB, Lurie S. The role of ethylene in the prevention of chilling injury in nectarines. Journal of Plant Physiology,2001,158:55-61.
258.Ziosi V, Scaramagli S, Bregoli AM, Biondi S, Torrigiani P. Peach (Prunus persica L.) fruit growth and ripening:transcript levels and activity of polyamine biosynthetic enzymes in the mesocarp. Journal of Plant Physiology,2003,160:1109-1115.
259.Zou TT, Rao JN, Liu L, Xiao L, Yu TX, Jiang P, Gorospe M, Wang JY. Polyamines Regulate the Stability of JunD mRNA by Modulating the Competitive Binding of Its 3' Untranslated Region to HuR and AUF1. Molecular and Cellular Biology,2010, 30:5021-5032.
2.柏亚男,吴茂森,何晨阳.水稻基因启动子OsBTF3p的克隆和启动活性分析.中国农业科学,2009,42：862-868.
3.黄培堂等译,Joseph S, David WR.分子克隆实验指南,第三版,北京：科学出版社,2002.
4.李建勇,卢钢,任彦.多胺在果实生长发育中的作用研究进展.果树学报,2005,3：256-260.
5.李敏,杨谦.一种高效构建同源重组DNA片段的方法——融合PCR.中国生物工程杂志,2007,27：53-58.
6.刘红霞.1-MCP, BTH和PHC对桃果(Prunus persica L.)采后衰老的调控作用及诱导抗病机理的研究.[博士学位论文].北京：中国农业大学,2004.
7.刘小花,张岚岚,朱长青,陈昆松,徐昌杰.Micro-Tom番茄矮化微型机制及其在植物功能基因组学研究中的应用.遗传,2008,30：1257-1264.
8.田世平主编.果蔬产品采后贮藏加工与包装技术指南.北京：中国农业出版社,2000,100.
9.汪祖华,庄恩及主编.中国果树志—桃卷.北京：中国林业出版社,2001,1-16.
10.王关林,方宏筠主编.植物基因工程.北京：科学出版社,2002.
11.王静.柑橘类植物非生物胁迫下多胺变化及两个多胺生物合成基因的克隆与鉴定.[博士学位论文].武汉：华中农业大学,2009.
12.吴延军.桃组织培养及遗传转化的研究.[博士学位论文].杭州：浙江大学,2004,13-19.
13. Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. The Plant Cell,2003,15:63-78.
14. Abeles FB, Morgan PW, Saltveit ME. Ethylene in plant biology.2nd ed. (San Diego, CA:Academic Press),1992.
15. Agarwal M, Hao Y, Kappoor A, Dong CH, Fujii H, Zheng X, Zhu JK. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. The Journal of Biological Chemistry,2006, 281:37636-37645.
16. Akiyama T, Jin S. Molecular cloning and characterization of an arginine decarboxylase gene up-regulated by chilling stress in rice seeding. Journal of Plant Physiology,2007,164:645-654.
17. Alcazar R, Altabella T, Marco F, Bortolotti C, Reymond M, Koncz C, Carrasco P, Tiburcio AF. Polyamines:molecules with regulatory functions in plant abiotic stress tolerance. Planta,2010,231:1237-1249.
18. Alcazar R, Cuevas JC, Planas J, Zarza X, Bortolotti C, Carrasco P, Salina J, Tiburcio AF, Altabella T, Integration of polyamines in the cold acclimation response, Plant Science,2011,180:31-38.
19. Alcazar R, Garcia-Martinez JL, Cuevas JC, Tiburcio AF, Altabella T. Overexpression of ADC2 in Arabidopsis induces dwarfism and late-flowering through GA deficiency. The Plant Journal,2005,43:425-436.
20. Alcazar R, Marco F, Cuevas JC, Patron M, Ferrando A, Carrasco P, Tiburcio AF, Altabella T. Involvement of polyamines in plant response to abiotic stress. Biotechnology Letters,2006,28:1867-1876.
21. Alonso JM, Hirayama T, Roman G, Nourizadeh S, Ecker JR. EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science,1999, 284:2148-2152.
22. An SH, Choi HW, Hong JK, Hwang BK. Regulation and function of the pepper pectin methylesterase inhibitor (CaPMEI1) gene promoter in defense and ethylene and methyl jasmonate signaling in plants. Planta,2009,230:1223-1237.
23. Anderson JP, Singh KB. Interactions of Arabidopsis and M. truncatula with the same pathogens differ in dependence on ethylene and ethylene response factors. Plant Signaling & Behavior,2011,6:551-552.
24. Argueso CT, Hansen M, Kieber JJ. Regulation of ethylene biosynthesis. Journal of Plant Growth Regulation,2007,26:92-105.
25. Arigita L, Tames RS, Gonzalez A. Ethylene biosynthesis and endogenous polyamines in relation to development of in vitro cultured kiwifruit explants. Functional Plant Biology,2004,31,603-609.
26. Arrom L, Munne-Bosch S. Hormonal changes during flower development in floral tissues of Lilium. Planta,2012, DOI:10.1007/s00425-012-1615-0.
27. Aziz A. Spermidine and related-metabolic inhibitors modulate sugar and amino acid levels in Vitis vinifera L.:possible relationships with initial fruitlet abscission. Journal of Experimental Botany,2003,54:355-363.
28. Bailey TA, Zhou X, Chen J, Yang Y. Role of ethylene, abscisic acid and MAP kinase pathways in rice blast resistance. Advances in Genetics, Genomics and Control of Rice Blast Disease,2009, Part Ⅱ,185-190.
29. Baninasab B, Rahemi M. Effect of exogenous polyamines on flower bud retention in pistachio (Pistacia vera L.) trees. Horticulture, Environment and Biotechnology, 2008,49:149-154.
30. Baron K, Stasolla C. The role of polyamines during in vivo and in vitro development. In vitro Cellular & Developmental Biology-Plant,2008,44:384-395.
31. Barry CS, Liop-Tous MI, Grierson D. The regulation of 1-aminocyclopropane-l-carboxylic acid synthase gene expression during the transition from system-1 to system-2 ethylene synthesis in tomato. Plant Physiology, 2000,123:979-986.
32. Bassett CL, Artlip TS, Callahan AM. Characterization of the peach homologue of the ethylene receptor, PpETR1, reveals some unusual features regarding transcript processing. Planta,2002,215:679-688.
33. Bell E, Malmberg RL. Analysis of a cDNA encoding arginine decarboxylase from oat reveals similarity to the Eschericbia coli arginine decarboxylase and evidence of protein processing. Molecular and General Genetics,1990,224:431-436.
34. Berrocal-Lobo M, Molina A. Ethylene response factor 1 mediates Arabidopsis resistance to the soilborne fungus Fusarium oxysporum. Molecular Plant-microbe Interactions,2004,17:763-770.
35. Bhatnagar-Mathur P, Vadez V, Sharma KK. Transgenic approaches for abiotic stress in plants:retrospect and prospects. Plant Cell and Report,2008,27:411-424.
36. Biasi R, Bagni N and Costa G. Endogenous polyamines in apple and their relationship to fruit set and fruit growth. Physiologia Plantarum,2006,73:201-205.
37. Binder BM. The ethylene receptors:complex perception for a simple gas. Plant Science,2008,175:8-17.
38. Bisson M, Bleckmann A, Allekotte S, Groth G. EIN2, the central regulator of ethylene signalling, is localised at the ER membrane where it interacts with the ethylene receptor ETR1. Biochemical Journal,2009,424,1:1-6.
39. Black DL, Graveley BR. Splicing bioinformatics to biology. Genome Biology,2006, 7:317.
40. Bleecker AB, Kende H. Ethylene:A gaseous signal molecule in plants. Annual Review of Cell and Developmental Biology,2000,16:1-18.
41. Bleecker AB. Ethylene perception and signaling:an evolutionary perspective. Trends in Plant Science.1999,4:269-274.
42. Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress:a review. Annals of Botany,2003,91:179-194.
43. Bortolotti C, Cordeiro A, Alcazar R, Borrell A, Culianez-macia FA, Tiburcio AF, Altabella T. Localization of arginine decarboxylase in tobacco plants. Physiologia Plantarum,2004,120:84-92.
44. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry,1976,72:248-254.
45. Brosche M, Overmyer K, Wrzaczek M, Kangajarvi J, Kangajarvi S. Stress signaling Ⅲ:Reactive Oxygen Species (ROS). Abiotic Stress Adaptation in Plants,2010, 1:91-102.
46. Cai M, Qiu DY, Yuan T, Ding XH, Li HJ, Duan L, Xu CG, Li XH, Wang SP. Identification of novel pathogen-responsive cis-elements and their binding proteins in the promoter of OsWRKYl3, a gene regulating rice disease resistance. Plant, Cell and Environment,2008,31:86-96.
47. Candan AP, Graell J, Larrigaudiere C. Roles of climacteric ethylene in the development of chilling injury in plums. Postharvest Biology and Technology,2008, 47:107-112.
48. Cao WH, Liu J, He XJ, Mu RL, Zhou HL, Chen SY, Zhang JS. Modulation of ethylene responses affects plant salt-stress responses. Plant Physiology,2007, 143:707-719.
49. Cao WH. Liu J. Zhou QY. Cao YR. Zheng SF. Du BX. Zhang JS. Chen SY. Expression of tobacco ethylene receptor NTHK1 alters plant responses to salt stress. Plant, Cell & Environment,2006,29:1210-1219.
50. Cao YR, Chen SY, Zhang JS. Ethylene signaling regulates salt stress response. Plant Signaling & Behavior,2008,3:761-763.
51. Capell T, Bassie L, Christou P. Modulation of the polyamine biosynthetic pathways in transgenic rice confers tolerance to drought stress. Proceeding of the National Academy of Sciences of the United States of America,2004,101:9909-9914.
52. Capell T, Escobar C, Liu H, Burtin D, Lepri O, Christou P. Over-expression of the oat arginine decarboxylase cDNA in transgenic rice (Oryza sativa L.) affects normal development patterns in vitro and results in putrescine accumulation in transgenic plants. Theoretical and Applied Genetics,1998,97:246-254. plants. Genes & Genomics,2009,31:107-118.
54. Carninci P, Sandelin A, Lenhard B, Katayama S, Shimokawa K, Ponjavic J, Semple CAM, Taylor MS, Engstrom PG, Frith MC, Forrest ARR, Alkema WB, Tan SL, Plessy C, Kodzius R, Ravasi T, Kasukawa T, Fukuda S, Kanamori-Katayama M, Kitazume Y, et al. Genome-wide analysis of mammalian promoter architecture and evolution. Nature Genetics,2006,38:626-635.
55. Chao Q, Rothenberg M, Solano R, Roman G, Terzaghi W, Ecker JR. Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins. Cell,1997,89:1133-1144.
56. Chen X, Wang Z, Gu R, Fu J, Wang J, Zhang Y, Wang M, Zhang J, Jia J, Wang G. Isolation of the maize Zpul gene promoter and its functional analysis in transgenic tobacco plants. Plant Cell Reports,2007,26:1555-1565.
57. Chen YF, Etheridge N, Schaller GE. Ethylene signal transduction. Annals of Botany, 2005,95:901-915.
58. Chen YF, Randlett MD, Findell JL, Schaller GE. Localization of the ethylene receptor ETR1 to the endoplasmic reticulum of Arabidopsis. The Journal of Biology Chemistry,2002,277:19861-19866.
59. Choudhury SR, Roy S, Das R, Sengupta DN. Differential transcriptional regulation of banana sucrose phosphate synthase gene in response to ethylene, auxin, wounding, low temperature and different photoperiods during fruit ripening and functional analysis of banana SPS gene promoter. Planta,2008,229:207-223.
60. Chuck G. Molecular mechanisms of sex determination in monoecious and dioecious plants. Advances in Botanical Research,2010,54:53-83.
61. Chung BYW, Simons C, Firth AE, Brown CM, Hellens RP. Effect of 5'UTR introns on gene expression in Arabidopsis thaliana. BMC Genomics,2006,7:20.
62. Ciardi J, Klee H, Regulation of ethylene-mediated responses at the level of the receptor, Annals of Botany,2001,88:813-822.
63. Ciardi JA, Tieman DM, Lund ST, Jones JB, Stall RE, Klee HJ. Response to Xanthomonas campestris pv. Vesicatoria in tomato involves regulation of ethylene receptor gene expression. Plant Physiology,2000,123:81-92.
64. Clancy M, Hannah LC. Splicing of the maize Sh1 first intron is essential for enhancement of gene expression, and a T-Rich motif increases expression without affecting splicing. Plant Physiology,2002,130:918-929.
65. Couee I, Hummel I, Sulmon C, Gouesbet G and Amrani AE. Involvement of polyamines in root development. Plant Cell, Tissue and Organ Culture,2004,76: 1-10.
66. Dhadi SR, Krom N, Ramakrishna W. Genome-wide comparative analysis of putative bidirectional promoters from rice, Arabidopsis and Populus. Gene,2009,429:65-73.
67. Dong CH, Rivarola M, Resnick JS, Maggin BD, Chang C. Subcellular co-localization of Arabidopsis RTE1 and ETR1 supports a regulatory role for RTE1 in ETR1 ethylene signalling. The Plant Journal,2008,53:275-286.
68. Doornbos RF, Geraats BP, Kuramae EE, van Loon LC, Bakker P. Effects of jasmonic acid, ethylene, and salicylic acid signaling on the rhizosphere bacterial community of Arabidopsis thaliana. Molecular Plant-Microbe Interactions,2011,24:395-407
69. Eapelund M, Jakobsen KS. Cloning and direct sequencing of plant promoters using primer-adaptor mediated PCR on DNA coupled to a magnetic solid phase. Biotechniques,1992,13:74-81.
70. Ecker JR, Davis RW. Plant defense genes are regulated by ethylene. Proceeding of the National Academy of Sciences of the United States of America,1987,84: 5202-5206.
71. Farooq M, Wahid A, Lee DJ. Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status, photosynthesis and membrane properties. Acta Physiologiae Plantarum,2009,31:937-945.
72. Filichkin SA, Priest HD, Givan SA, Shen R, Bryant DW, Fox SE, Wong WK, Mockler TC. Genome-wide mapping of alternative splicing in Arabidopsis thaliana. Genome Research,2010,20:45-58.
73. Fraga MF, Berdasco M, Diego LB, Rodriguez R, Canal MJ. Changes in polyamine concentration associated with aging in Pinus radiata and Prunus persica. Tree Physiology,2004,24:1221-1226.
74. Gamalero E, Glick BR. Ethylene and abiotic stress tolerance in plants. Environmental Adaptations and Stress Tolerance of Plants in the Era of Climate Change,2012,395-412.
75. Gao Z, Chen YF, Randlett MD, Zhao XC, Findell JL, Kieber JJ, Schaller GE. Localization of the Raf-like kinase CTR1 to the endoplasmic reticulum of Arabidopsis through participation in ethylene receptor signaling complexes. The Journal of Biological Chemistry,2003,278:34725-34732.
76. Ge C, Cui X, Wang Y, Hu Y, Fu Z, Zhang D, Cheng Z, Li J. BUD2, encoding an S-adenosylmethionine decarboxylase, is required for Arabidopsis growth and development. Cell Research,2006,16:446-456.
77. Gemperlova L, Eder J, Cvikrova M. Polyamine metabolism during the growth cycle of tobacco BY-2 cells. Plant Physiology and Biochemistry,2005,43:375-381.
78. Gill SS, Tuteja N. Polyamines and abiotic stress tolerance in plants. Plant Signaling & Behavior,2010,5:26-33.
79. Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry,2010, 48:909-930.
80. Giovannoni J. Molecular biology of fruit maturation and ripening. Annual Review of Plant Physiology and Plant Molecular Biology,2001,52:725-749.
81. Gomez MD, Beltran JP, and Canas LA. The pea END] promoter drives anther-specific gene expression in different plant species. Planta,2004,219: 967-981.
82. Gowik U, Burscheidt J. Akyildiz M., Schlue U., Koczor M., Streubel M., and Westhotf P. cis-regulatory elements for mesophyll-specific gene expression in the C4 plant Flaveria trinervia, the promoter of the C4 phosphoenolpyruvate carboxylase gene. Plant Cell,2004,16:1077-1090.
83. Grefen C, Stadele K, Ruzicka K, Obrdlik P, Harter K, Horak J. Subcellular localization and in vivo interactions of the arabidopsis thaliana ethylene receptor family members. Molecular Plant,2008,1:308-320.
84. Groppa MD, Benavides MP. Polyamines and abiotic stress:recent advances. Amino Acids,2008,34:35-45.
85. Guo DP, Sun YZ, Chen ZJ. Involvement of polyamines in cytoplasmic male sterility of stem mustard (Brassica juncea var. tsatsai). Plant Growth Regulation,2003, 41:33-40.
86. Gutterson N, Reuber TL. Regulation of disease resistance pathways by AP2/ERF transcription factors. Current Opinion in Plant Biology,2004,7:465-471.
87. Hamilton DA, Schwarz YH, Mascarenhas JP. A monocot pollen-specific promoter contains separable pollen-specific and quantitative elements. Plant Molecular Biology,1998,38:663-669.
88. Hanfrey C, Sommer S, Mayer MJ, Burtin D, Michael AJ. Arabidopsis polyamine biosynthesis:absence of ornithine decarboxylase and the mechanism of arginine decarboxylase activity. Plant Journal,2001,27:551-560.
89. Hao YJ, Kitashiba H, Honda C, Nada K, Moriguchi T. Expression of arginine deccarboxylase and ornithine decarboxylase genes in apple cells and stressed shoots. Journal of Experimental Botany,2005,56:1105-1115.
90. He LX, Ban Y, Inoue H, Matsuda N, Liu J, Moriguchi T. Enhancement of spermidine content and antioxidant capacity in transgenci pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis. Phytochemistry,2008, 69:2133-2141.
91. Higo K, Ugawa Y, Iwamoto M, Korenaga T. Plant cis-acting regulatory DNA elements (PLACE) database:1999. Nucleic Acids Research,1999,27:297-300.
92. Hirano K, Ueguchi-Tanaka M, Matsuoka M. GID1-mediated gibberellin signaling in plants. Trends in Plant Science,2008,13:192-199.
93. Hoth S, Niedermeier M, Feuerstein A, Hornig J, Sauer N. An ABA-responsive element in the AtSUC1 promoter is involved in the regulation of AtSUC1 expression. Planta,2010,232:911-923.
94. Hua J, Chang C, Sun Q, Meyerowitz EM. Ethylene insensitivity conferred by Arabidopsis ERS gene. Science,1995,269:1712-1714.
95. Hua J, Meyerowitz EM. Ethylene responses are negatively regulated by a receptor gene family in Arabidopsis thaliana. Cell,1998,94:261-271.
96. Huang CK, Chang BS, Wang KC, Her SJ, Chen TW, Chen YA, Cho CL, Liao LJ, Huang KL, Chen WS, Liu ZH. Changes in polyamine pattern are involved in floral initiation and development in Pelianthes tuberosa. Journal of Plant Physiology,2004, 161:709-713.
97. Huang XS, Liu JH, Chen XJ. Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes. BMC Plant Biology,2010,10:230, doi:10.1186/1471-2229-10-230.
98. Huang XS, Luo T, Fu XZ, Fan QJ, Liu JH. Cloning and molecular characterization of a mitogen-activated protein kinase gene from Poncirus trifoliata whose ectopic expression confers dehydration/drought tolerance in transgenic tobacco. Journal of Experimental Botany,2011,62:5191-5206.
99. Huang Y, Li H, Hutchison CE, Laskey J, Kieber JJ. Biochemical and functional analysis of CTR1, a protein kinase that negatively regulates ethylene signaling in Arabidopsis. Plant Journal,2003,33:221-233.
100.Hummel I, Bourdais G, Gouesbet G, Couee I, Malmberg RL, Amrani AE. Differential gene expression of ARGININE DECARBOXYLASE ADC1 and ADC2 in Arabidopsis thaliana:characterization of transcriptional regulation during seed germination and seedling development. New Phytologist,2004,163:519-531.
101.Hussain SS, Ali M, Ahmad M, Siddique KHM. Polyamines:Natural and engineered abiotic and biotic stress tolerance in plants. Biotechnology Advances,2011,29: 300-311.
102.Imai A, Matsuyama T, Hanzawa Y, Akiyama T, Tamaoki M, Saji H, Shirano Y, Kato T, Hayashi H, Shibata D, Tabata S, Komeda Y, Takahashi T. Spermidine synthase genes are essential for survival of Arabidopsis. Plant Physiology,2004, 135:1565-1573.
103.Ish-Shalom M, Dahan Y, Maayan I, Irihimovitch V. Cloning and molecular characterization of an ethylene receptor gene, MiERS1, expressed during mango fruitlet abscission and fruit ripening. Plant Physiology and Biochemistry,2011, 49:931-936.
104.Ivanchenko MG, Muday GK, Dubrovsky JG. Ethylene-auxin interactions regulate lateral root initiation and emergence in Arabidopsis thaliana. The Plant Journal,2008, 55:335-347.
105.Iwai T, Miyasaka A, Seo S, Ohashi Y. Contribution of ethylene biosynthesis for resistance to blast fungus infection in young rice plants. Plant Physiology,2006, 142:1202-1215.
106.Johnson PR, Ecker JR. The ethylene gas signal transduction pathway:A molecular perspective. Annual Review of Genetics,1998,32:227-254.
107.Juven-Gershon T, Hsu JY, Theisen JWM, Kadonaga JT. The RNA polymerase Ⅱ core promoter-the gateway to transcription. Current Opinion in Cell Biology,2008, 20:253-259.
108.Kakkar RK, Sawhney VK. Polyamine research in plants-a changing perspective. Physiologia Plantarum,2002,116:281-292.
109.Karthikeyan AS, Ballachanda DN, Raghothama KG. Promoter deletion analysis elucidates the role of cis elements and 5'UTR intron in spatiotemporal regulation of AtPht1;4 expression in Arabidopsis. Physiologia Plantarum,2009,136:10-18.
110.Katerova ZI, Todorova D. Endogenous polyamines lessen membrane damages in pea plants provoked by enhanced ultraviolet-C radiation. Plant Growth Regulation,2009, 57:145-152.
111.Kaur-Sawhney R, Tiburcio AF, Altabella T, Galston AW. Polyamines in plants:A overview. Journal of Cell and Molecular Biology,2003,2:1-12.
112.Kazmierczak A. Giberellic acid and ethylene control male sex determination and development of anemia phyllitidis gametophytes. Working With Ferns,2010, Part I 49-65.
113.Kendrick MD, Chang C. Ethylene signaling:new levels of complexity and regulation. Current Opinion in Plant Biology,2008,11:479-485.
114.Kepczynski J, Kepczynska E. Manipulation of ethylene biosynthesis. Acta Physiologiae Plantarum,2005,27:213-220.
115.Kieber JJ, Rothenberg M, Roman G, Feldman KA, Ecker JR. CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the Raf family of protein kinases. Cell,1993,72:427-441.
116.Kizis D, Lumbreras V, Pages M.Role of AP2/EREBP transcription factors in gene regulation during abiotic stress. FEBS Letters,2001,498:187-189.
117.Kizis D, Pages M. Maize DRE-binding proteins DBF1 and DBF2 are involved in rabl7 regulation through the drought-responsive element in an ABA-dependent pathway. The Plant Journal,2002,30:679-689.
118.Klee HJ, Tieman D. The tomato ethylene receptor family:Form and function. Physiologia Plantarum,2002,115:336-341.
119.Kobayashi T., Nakayama Y., Itai R.N., Nakanishi H., Yoshihara T., Mori S., and Nishizawa N.K. Identification of novel cis-acting elements, IDE1 and IDE2, of the barley IDS2 gene promoter conferring iron-deficiency-inducible, root-specific expression in heterogeneous tobacco plants. Plant Journal,2003,36:780-793.
120.Kropat J, Tottey S, Birkenbihl RP, Depege N, Huijser P, Merchant S. A regulator of nutritional copper signaling in chlamydomonas is an SBP domain protein that recognizes the GTAC core of copper response element. Proceeding of the National Academy of Sciences of the United States of America,2005,102:18730-18735.
121.Kunkel BN, Brooks DM. Cross talk between signaling pathways in pathogen defense. Current Opinion in Plant Biology,2002,5:325-331.
122.Kunkel BN, Brooks DM. Cross talk between signaling pathways in pathogen defense. Current Opinion in Plant Biology,2002,5:325-331.
123.Kusano T, Berberich T, Tateda C, Takahashi Y. Polyamines:essential factors for growth and survival. Planta,2008,228:367-381.
124.Laquitaine L, Gomes E, Francois J, Marchive C, Pascal S, Hamdi S, Atanassova R, Delrot S, Coutos-Thevenot P. Molecular basis of ergosterol-induced protection of grape against botrytis cinerea:induction of type I LTP promoter activity, WRKY, and stilbene synthase gene expression. Molecular Plant-Microbe Interactions.2006, 19:1103-1112.
125.Leclercq J, Adams-Phillips LC, Zegzouti H, Jones B, Latche A, Giovannonni JJ, Pech JC, Bouzayen M. LeCTR1, a tomato CTR1-like gene, demonstrates ethylene signaling ability in Arabidopsis and novel expression patterns in tomato. Plant Physiology,2002,130:1132-1142.
126.Lee SC, Kim DS, Kim NH, Hwang BK. Functional analysis of the promoter of the pepper pathogen-induced gene, CAPIP2, during bacterial infection and abiotic stresses. Plant Science,2007,172:236-245.
127.Lelievre JM, Latche A, Jones B, Bouzayen M, Pech JC. Ethylene and fruit ripening. Physiologia Plantarum,1997,101:727-739.
128.Leon-Reyes A, Du Y, Koornneef A, Proietti S, Korbes AP, Memelink J, Pieterse C, Ritsema T. Ethylene signaling renders the jasmonate response of Arabidopsis insensitive to future suppression by salicylic acid. Molecular Plant-Microbe Interactions,2010,23:187-197.
129.Lescot M, Dehais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouze P, Rombauts S. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Research, 2002,30:325-327.
130.Lewis MW, Leslie ME, Liljegren SJ. Plant separation:50 ways to leave your mother. Current Opinion in Plant Biology,2008,9:59-65.
131.Li CZ, Jiao J, Wang GX. The important roles of reactive oxygen species in the relationship between ethylene and polyamines in leaves of spring wheat seedlings under root osmotic stress. Plant Science,2004,166:303-315.
132.Li Y, Wu Z, Ma N, Gao J. Regulation of the rose RH-PIP2;1 promoter by hormones and abiotic stresses in Arabidopsis. Plant Cell Reports,2009,28:185-196.
133.Liang Y, Bae H, Kang S, Lee T, Kim M, Kim Y, Ha S. The Arabidopsis beta-carotene hydroxylase gene promoter for a strong constitutive expression of transgene. Plant Biotechnology Reports,2009,3:325-331.
134.Lim PO, Kim HJ, Nam HG. Leaf senescence. Annual Review of Plant Biology,2007, 58:115-136.
135.Lin Z, Zhong S, Grierson D. Recent advances in ethylene research. Journal of Experimental Botany,2009,60:3311-3336.
136.Linkies A, Leubner-Metzger G. Beyond gibberellins and abscisic acid:how ethylene and jasmonates control seed germination. Plant Cell Reports,2012,31:253-270.
137.Liu H, Creech RG, Jenkins JN, Ma D. Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3. Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids,2000,1487:106-111.
138.Liu JH, Ban Y, Wen XP, Nakajima I, Moriguchi T. Molecular cloning and expression analysis of an arginine decarboxylase gene from peach(Prunus persica). Gene,2009, 429:10-17.
139.Liu JH, Honda C, Moriguchi T. Involvement of polyamine in floral and fruit development. Japan agricultural research quarterly,2006a,40:51-58.
140.Liu JH, Inoue H, Moriguchi T. Salt stress-mediated changes in free polyamine titers and expression of genes responsible for polyamine biosynthesis of apple in vitro shoots. Environmental and Experimental Batany,2008,62:28-35.
141.Liu JH, Kitashiba H, Wang J, Ban Y, Moriguchi T. Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnology,2007, 24:117-126.
142.Liu JH, Moriguchi T. Changes in free polyamines and gene expression during peach flower development. Biologia Plantarum,2007,51:530-532.
143.Liu JH, Nada K, Honda C, Kitashiba H, Wen XP, Pang XM, Moriguchi T. Polyamine biosynthesis of apple callus under salt stress:involvement of ADC pathway in stress response. Journal of Experimental Botany,2006c,57:2589-2599.
144.Liu JH, Nada K, Pang XM, Honda C, Kitashiba H, Moriguchi T. Role of polyamines in peach fruit development and storage. Tree Physiology,2006b,26:791-798.
145.Liu JH, Nakajima I, Moriguchi T. Effects of salt and osmotic stresses on free polyamine content and expression of polyamine biosynthetic genes in Vitis vinifera. Biologia Plantarum,2011,55:340-344.
146.Liu K, Fu HH, Bei QX, Luan S. Inward potassium channel in guard cells as a target for polyamine regulation of stomatal movements. Plant Physiology,2000, 124:1315-1325.
147.Lorenzo O, Piqueras R, Sanchez-Serrano JJ, Solano R. ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell,2003,15:165-178.
148.Lu J, Sivamani E, Azhakanandam K, Samadder P, Li X, Qu R. Gene expression enhancement mediated by the 5'UTR intron of the rice rubi3 gene varied remarkably among tissues in transgenic rice plants. Molecular Genetics and Genomics,2008, 279:563-572.
149.Ludwig AA, Saitoh H, Felix G, Freymark G, Miersch O, Wasternack C, Boller T, Jones JD, Romeis T. Ethylene-mediated cross-talk between calcium-dependent protein kinase and MAPK signaling controls stress responses in plants. Proceeding of the National Academy of Sciences of the United States of America,2005, 102:10736-10741.
150.Lurie S, Crisosto CH. Chilling injury in peach and nectarine. Postharvest Biology and Technology,2005,37:195-208.
151.Ma B, Cui ML, Sun HJ, Takada K, Mori H, Kamada H, Ezura H. Subcellular localization and membrane topology of the melon ethylene receptor CmERS1. Plant Physiology,2006,141:587-597.
152.ManaVella PA, Dezar CA, Ariel FD, Chan RL. Two ABREs, two redundant root-specific and one W-box cis-acting elements are functional in the sunflower HAHB4 promoter. Plant Physiology Biochemistry,2008,46:860-867.
153.Mao X, Zhang H, Tian S, Chang X, Jing R. TaSnRK2.4, an SNFl-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis. Journal of Experimental Botany,2010, 61:683-696.
154.Mascarenhas D, Mettler IJ, Pierce DA, Lowe HW. Intron-mediated enhancement of heterologous gene expression in maize. Plant Molecular Biology,1990,15:913-920.
155.Masgrau C, Altabella T, Farras R, Flores D, Thompson AJ, Besford RT, Tiburcio AF. Inducible overexpression of oat arginine decarboxylase in transgenic tobacco plants. The Plant Journal,1997,11:465-473.
156.Masura SS, Parveez GKA, Ti LLE. Isolation and characterization of an oil palm constitutive promoter derived from a translationally control tumor protein (TCTP) gene. Plant Physiology and Biochemistry,2011,49:701-708.
157.McManus MT, Harpaz-Saad S, Yoon GM, Mattoo AK, Kieber JJ. The formation of ACC and competition between polyamines and ethylene for SAM. Annual Plant Reviews,2012,44:DOI:10.1002/9781118223086.ch3.
158.Mehta RA, Cassol T, Li N, Ali N, Handa AK, Mattoo AK. Engineered polyamine accumulation in tomato enchances phytonutrient content, juice quality, and vine life. Nature Biotechnology,2002,20:613-618.
159.Meisel L, Fonseca B, Gonzalez S, Baezayates R, Cambiazo V, Campos R, Gonzalez M, Orellana A, Retamales J, Silva H. A rapid and efficient method for purifying high quality total RNA from peaches(Prunus persica) for functional genomics analyses. Biological Research,2005,38:83-88.
160.Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R. Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant, Cell & Environment,2010, 33:453-467.
161.Minocha R, Minocha SC, Long S. Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.). In Vitro Cellular & Developmental Biology-Plant,2004,40:572-580.
162.Mirdehghan SH, Rahemi M, Martinez-Romero D, Guillen F, Valverde JM, Zapata PJ, Serrano M, Valero D. Reduction of pomegranate chilling injury during storage after heat treatment:role of polyamines. Postharvest Biology and Technology,2007, 44:19-25.
163.Mo H, Pua EC. Up-regulation of arginine decarboxylase gene expression and accumulation of polyamines in mustard (Brassica juncea) in response to stress. Physiologia Plantarum,2002,114:439-449.
164.Moon H, Callahan AM. Developmental regulation of peach ACC oxidase promoter-GUS fusions in transgenic tomato fruits. Journal of Experimental Botany, 2004,55:1519-1528.
165.Morgan PW, Drew MC. Ethylene and plant responses to stress. Physiologia Plantarum,1997,100:620-630.
166.Nam KH, Lee SH, Lee J. Differential expression of ADC mRNA during development and upon acid stress in Soybean (Glycine mas) Hypocotyls. Plant Cell Physiology,1997,38:1156-1166.
167.Nambeesan S, Qamar SA, Laluk K, Mattoo AK, Mickelbart MV, Ferruzzi MG, Mengiste T, Handa AK. Polyamines Attenuate Ethylene-Mediated Defense Responses to Abrogate Resistance to Botrytis cinerea in Tomato. Plant Physiology, 2012,158:1034-1045.
168.Nayyar H, Chander S. Protective effects of polyamines against oxidative stress induced by water and cold stress in chickpea. Journal of Agronomy and Crop Science,2004,190:355-365.
169.Negi S, Ivanchenko MG, Muday GK. Ethylene regulates lateral root formation and auxin transport in Arabidopsis thaliana. The Plant Journal,2008,55:175-187.
170.Nishiuchi T, Shinshi H, Suzuki K. Rapid and transient activation of transcription of the ERF3 gene by wounding in tobacco leaves-Possible involvement of NtWRKYs and autorepression. Journal of Biological Chemistry,2004,279:55355-55361.
171.Nolke G, Schneider B, Fischer R, Schillberg S. Immunomodulation of polyamine biosynthesis in tobacco plants has a significant impact on polyamine levels and generates a dwarf phenotype. Plant Biotechnology Journal,2005,3:237-247.
172.O'Malley RC, Rodriguez FI, Esch JJ, Binder BM, O'Donnell P, Klee HJ, Bleecker AB. Ethylene-binding activity, gene expression levels, and receptor system output for ethylene receptor family members from Arabidopsis and tomato. The Plant Journal, 2005,41:651-65.
173.Pandey S, Ranade SA, Nagar PK, Kumar N. Role of polyamines and ethylene as modulators of plant senescence. Journal of Biosciences,2000,25,291-299.
174.Pang JH, Ma B, Sun HJ, Ortiz GI, Imanishi S, Sugaya S, Gemma H, Ezura H. Identification and characterization of ethylene receptor homologs expressed during fruit development and ripening in persimmon(Diospyros kaki Thumb.), Postharvest Biology and Technology,2007,44:195-203.
175.Pang XM, Zhang ZY, Wen XP, Ban Y, Moriguchi T. Polyamines, All-purpose players in response to environment stresses in plants. Plant Stress,2007,1:173-188.
176.Paschalidis KA, Roubelakis-Angelakis KA. Spatial and temporal distribution of polyamine levels and polyamine anabolism in different organs/tissues of the tobacco plant. Correlations with age, cell division/expansion, and differentiation. Plant Physiology,2005,138:142-152.
177.Paschalidis KA, Roubelakis-Angelakis KA. Spatial and temporal distribution of polyamine levels and polyamine anabolism in different organs/tissues of the tobacco plant. Correlations with age, cell division/expansion, and differentiation. Plant Physiology,2005,138:142-152.
178.Paul V, Pandey R, Srivastava GC. Tomato fruit ripening:regulation of ethylene production and its response. Indian Journal of Plant Physiology,2011,16:117-131
179.Pech JC, Purgatto E, Bouzayen M and Latche A. Ethylene and Fruit Ripening. Annual Plant Reviews,2012, Volume 44:The Plant Hormone Ethylene (doi:10.1002/9781118223086.chl1).
180.Peleg Z, Blumwald E. Hormone balance and abiotic stress tolerance in crop plants. Current Opinion in Plant Biology,2011,14:290-295.
181.Perez-Amador MA, Leon J, Green PJ, Carbonell J. Induction of the arginine decarboxylase ADC2 gene provides evidence for the involvement of polyamines in the wound response in arabidopsis. Plant Physiology,2002,130:1454-1463.
182.Poresbski S, Bailey GL, Baum RB. Modification of CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter,1997,12:8-15.
183.Potenza C, Aleman L, Sengupta-Gopalan C. Targeting transgene expression in research, agricultural, and environmental applications:Promoters used in plant transformation. In Vitro Cellular and Development Biology Plant,2004,40:1-22.
184.Primikirios NI, Roubelakis-Angelakis KA. Cloning and expression of an arginine decarboxylase cDNA from Vitis vinifera L. cell-suspension cultures. Planta,1999, 208:574-582.
185.Quinet M, Ndayiragije A, Lefevre I, Lambillotte B, Dupont-Gillain CC, Lutts S. Putrescine differently influences the effect of salt stress on polyamine metabolism and ethylene synthesis in rice cultivars differing in salt resistance. Journal of Experimental Botany,2010,61:2719-2733.
186.Rajesh MK, Radha E, Karun A, Parthasarathy VA. Plant regeneration from embryo-derived callus of oil palm-the effect of exogenous polyamines. Plant Cell, Tissue and Organ Culture,2003,75:41-47.
187.Rakitin VY, Prudnikova ON, Rakitina TY, Karyagin VV, Vlasov PV, Novikova GV, Moshkov IE. Interaction between ethylene and ABA in the regulation of polyamine level in Arabidopsis thaliana during UV-B stress. Russian Journal of Plant Physiology,2009,56:147-153.
188.Rasori A, Bertolasi B, Furini A, Bonghi C, Tonutti P, Ramina A. Functional analysis of peach ACC oxidase promoters in transgenic tomato and in ripening peach fruit. Plant Science,2003,165:523-530.
189.Rasori A, Ruperti B, Bonghi C, Tonutti P, Ramina A. Characterization of two putative ethylene receptor genes expressed during peach fruit development and abscission. Journal of Experimental Botany,2002,53:2333-2339.
190.Rastogi R, Dulson J, Rothstein SJ. Cloning of tomato (Lycopersicon esculentum Mill.) arginine decarboxylase gene and its expression during fruit ripening. Plant Physiology,1993,103:829-834.
191.Rawat R, Xu ZF, Yao KM, Chye ML, Identification of cis-elements for ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase. Plant Molecular Biology,2005,57:629-643.
192.Rhee HJ, Kim EJ, Lee JK. Physiological polyamines:simple primordial stress molecules. Journal of Cellular and Molecular Medicine,2007,11:685-703.
193.Richards DE, King KE, Ait-ali T, Harberd NP. How gibberellin regulates plant growth and development:a molecular genetic analysis of gibberellin signaling. Annual Review of Plant Physiology and Plant Molecular Biology,2001,52:67-88.
194.Romero-Puertas MC, Rodriguez-Serrano M, Corpas FJ, Gomez M, Rio Del LA, Sandalio LM. Cadmium-induced subcellular accumulation of O2- and H2O2 in pea leaves. Plant Cell & Environment,2004,27:1122-1134.
195.Roy M, Wu R. Arginine decarboxylase transgene expression and analysis of environmental stress tolerance in transgenic rice. Plant Science,2001,160:869-875.
196.Rushton PJ, Reinstadler A, Lipka V, Lippok B, Somssich LE. Synthetic plant promoters containing defined regulatory elements provide novel insights into pathogen- and wound-induced signaling. Plant Cell,2002,14:749-762.
197.Sairam PK, Tyagi A. Physiology and molecular biology of salinity stress tolerance in plants. Current Science,2004,86:407-421.
198.Salazar M, Gonzalez E, Casaretto JA, Casacuberta JM, Ruiz-Lara S. The promoter of the TLC1.1 retrotransposon from Solanum chilense is activated by multiple stress-related signaling molecules. Plant Cell Reports,2007,26:1861-1868.
199.Samadder P, Sivamani E, Lu J, Li X, Qu R. Transcriptional and post-transcriptional enhancement of gene expression by the 5'UTR intron of rice rubi3 gene in transgenic rice cells. Molecular Genetics Genomics,2008,279:429-439.
200.Schaller GE, Kieber JJ.2002. Ethylene. In:The Arabidopsis book. Rockville, MD: American Society of Plant Biologists (Online: http://www.aspb.org/publications/arabidopsis/).
201.Schaller GE. Ethylene and the regulation of plant development. BMC Biology,2012, 10:9.
202.Seiler N, Raul F. Polyamines and apoptosis. Journal of Cellular and Molecular Medicine,2005,9:623-642.
203.Shahmuradov IA, Gammerman AJ, Hancock JM, Bramley PM, Solovyev VV. PlantProm:a database of plant promoter sequences. Nucleic Acids Research,2003, 31:114-117.
204.Shi J, Fu XZ, Peng T, Huang XS, Fan QJ, Liu JH. Spermine pretreatment confers dehydration tolerance of citrus in vitro plants via modulation of antioxidative capacity and stomatal response. Tree Physiology,2010,30:914-922.
205.Simpson SD, Nakashima K, Narusaka Y, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Two different novel cis-acting elements of erdl, a clpA homologous Arabidopsis gene function in induction by dehydration stress and dark-induced senescence. The Plant Journal,2003,33:259-270.
206.Singer SD, Hily JM, Cox KD. The sucrose synthase-1 promoter from Citrus sinensis directs expression of the β-glucuronidase reporter gene in phloem tissue and in response to wounding in transgenic plants. Planta,2011,234:623-637.
207.Stepanova AN, Alonso JM. Ethylene signaling and response:where different regulatory modules meet. Current Opinion in Plant Biology,2009,12:548-555.
208.Sun HJ, Uchii S, Watanabe S, Ezura H. A highly efficient transformation protocol for Micro-Tom, A model cultivar for tomato functional genomics. Plant Cell Physiology,2006,47:426-431.
209.Sunilkumar G, Mohr L A, Lopata-Finch E, Emani C, Rathore K. Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP. Plant Molecular Biology,2002,50:463-74.
210.Swarup R, Perry P, Hagenbeek D, Van Der Straeten D, Beemster GT, Sandberg G, Bhalerao R, Ljung K, Bennett MJ. Ethylene upregulates auxin biosynthesis in Arabidopsis seedlings to enhance inhibition of root cell elongation. The Plant Cell, 2007,19:2186-2196.
211.Swiatecka-Hagenbruch M, Liere K, Borner T. High diversity of plastidial promoters in Arabidopsis thaliana. Molecular Genetics Genomics,2007,277:725-734.
212.Tadolini B. Polyamine inhibition of lipoperoxidation. The influence of polyamines on iron oxidation in the presence of compounds mimicking phospholipid polar heads. Biochemical Journal,1988,249:33-36.
213.Takahashi T, Kakehi JI. Polyamines:ubiquitous polycations with unique roles in growth and stress responses. Annals of Botany,2010,105:1-6.
214.Tang CF, Liu YG, Zeng GM, Li X, Xu WH, Li CF, Yuan XZ. Effects of exogenous spermidine on antioxidant system responses of Typha latifolia L. under Cd2+stress. Journal of Integrative Plant Biology,2005,47:428-434.
215.Tang W, Newton RJ. Polyamines promote root elongation and growth by increasing root cell division in regenerated Virginiapine (Pinusvirgiana Mill.) plantlets. Plant Cell Reports,2005,24:581-589.
216.Tanguy JM. Metabolism and function of polyamines in plants:recent development (new approaches). Plant Growth Regulation,2001,34:135-148.
217.Tieman DM, Ciardi JA, Taylor MG, Klee HJ. Members of the tomato LeEIL (EIN3-like) gene family are functionally redundant and regulate ethylene responses throughout plant development. The Plant Journal,2001,26:47-58.
218.Tonutti P, Bonghi C, Ramina A. Fruit firmness and ethylene biosynthesis in three cultivars of peach (Prunus persical L. Batsch). Journal of Horticultural Science, 1996,71(1):141-147.
219.Toquin V, Grausem B, Geotfroy P, Legrand M. Structure of the tobacco caffeic acid O-methyltransferase (COMT) Ⅱ gene:identification of promoter sequences involved in gene inducibility by various stimuli. Plant Molecular Biology,2003,52:495-509.
220.Trainotti L, Bonghi C, Ziliotto F, Zanin D, Rasori A, Casadoro G, Ramina A, Tonutti P. The use of microarray μPEACH1.0 to investigate transcriptome changes during transition from pre-climacteric to climacteric phase in peach fruit. Plant Science, 2006,170:606-613.
221.Urano K, Hobo T, Shinozaki K. Arabidopsis ADC genes involved in polyamine biosynthesis are essential for seed development. Federation of European Biochemical Societies Letters,2005,579:1557-1564.
222.Urano K, Yoshiba Y, Nanjo T, Ito T, Yamaguchi-Shinozaki K, Shinozaki K. Arabidopsis stress-inducible gene for arginine decarboxylase AtADC2 is required for accumulation of putrescine in salt tolerance. Biochemical and Biophysical Research Communications,2004,313:369-375.
223.Valero D. The role of polyamines on fruit ripening and quality during storage:what is new. Acta Horticulturae Home,2009,884:199-205.
224.Venter. Synthetic promoters:genetic control through cis engineering. Trends in Plant Science,2007,12:118-124.
225.Vera-Sirera F, Minguet EG, Singh SK, Ljung K, Tuominen H, Blazquez MA, Carbonell J. Role of polyamines in plant vascular development. Plant Physiology and Biochemistry,2010,48:534-539.
226.Verma S, Mishra SN. Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system. Journal of Plant Physiology,2005, 162:669-677.
227.Vickers CE, Xue G, Gresshoff PM. A high-level endosperm-specific expression in an novel cis-acting element, ESP, contributes to oat globulin promoter. Plant Molecular Biology,2006,62:195-214.
228.Wang DH, Li F, Duan QH, Han T, Xu ZH, Bai SN. Ethylene perception is involved in female cucumber flower development. The Plant Journal,2010,61:862-872.
229.Wang J, Sun PP, Chen CL, Wang Y, Fu XZ, Liu JH. An arginine decarboxylase gene PtADC from Poncirus trifoliata confers abiotic stress tolerance and promotes primary root growth in Arabidopsis. Journal of Experimental Botany,2011, 62:2899-2914.
230.Wang KLC, Li H, Ecker JR. Ethylene biosynthesis and signaling networks. Plant Cell (Suppl),2002,14:S131-S151.
231.Wang P, Zhang B, Li X, Xu CJ, Yin XR, Shan LL, Ferguson I, Chen KS. Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit. Journal of Experimental Botany,2010,61:179-190.
232.Wang W, Esch JJ, Shiu SH, Agula H, Binder BM, Chang C, Patterson SE, Bleecker AB. Identification of important regions for ethylene binding and signaling in the transmembrane domain of the ETR1 ethylene receptor of Arabidopsis. The Plant Cell,2006,18:3429-3442.
233.Wang W, Hall AE, O'Malley R, Bleecker AB. Canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor from Arabidopsis is not required for signal transmission. Proceeding of the National Academy of Sciences of the United States of America,2003,100:352-357.
234.Watson MW, Malmberg RL. Regulation of Arabidopsis thaliana (L.) Heynh arginine decarboxylase by potassium deWciency stress. Plant Physiology,1996, 111:1077-1083.
235.Watson MW, Yu W, Galloway GL, Malmberg RL. Isolation and characterization of a second arginine decarboxylase cDNA from Arabidopsis (PGR97-114). Plant Physiology,1997,114:1569.
236.Wen XP, Ban Y, Inoue H, Matsuda N, Moriguchi T. Spermidine levels are implicated in heavy metal tolerance in a spermidine synthase overexpressing transgenic European pear by exerting antioxidant activities. Transgenic Research,2009, 19:91-103.
237.Wilkinson S, Davies WJ. Drought, ozone, ABA and ethylene:new insights from cell to plant to community. Plant, Cell & Environment,2010,33:510-525.
238.Wolukau JN, Zhang SL, Xu GH, Chen D. The effect of temperature, polyamines and polyamine synthesis inhibit or on in vitro pollen germination and pollen tube growth of Prunus mume. Scientia Horticulturae,2004,99:289-299.
239.Wu XF, Wang CL, Xie EB, Gao Y, Fan YL, Liu PQ, Zhao KJ. Molecular cloning and characterization of the promoter for the multiple stress-inducible gene BjCHI1 from Brassica juncea. Planta,2009,229:1231-1242.
240.Xie C, Zhang JS, Zhou HL, Li J, Zhang ZG, Wang DW, Chen SY. Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco. The Plant Journal,2003,33:385-393.
241.Xie F, Liu Q, and Wen CK. Receptor signal output mediated by the ETR1 N-terminus is primarily subfamily I receptors-dependent. Plant Physiology,2006, 142:492-508.
242.Xu X, Shi G, Ding C, Xu Y, Zhao J, Yang H, Pan Q. Regulation of exogenous spermidine on the reactive oxygen species level and polyamine metabolism in Alternanthera philoxeroides (Mart.) Griseb under copper stress. Plant Growth Regulation,2011,63:251-258.
243.Yamaguchi K, Takahashi Y, Berberich T, Imai A, Takahashi T, Michael AJ, Kusano T. A protective role for the polyamine spermine against drought stress in Arabidopsis. Biochemical and Biophysical Research Communications,2007,352:486-490.
244.Yamaguchi S.Gibberellin metabolism and its regulation. Annual Review of Plant Biology,2008,59:225-251.
245.Yang J, Zhang J, Liu K, Wang Z, Liu L. Involvement of polyamines in the drought resistance of rice. Journal of Experimental Botany,2007,58:1545-1555.
246.Yang SF, Hoffman NE. Ethylene biosynthesis and its regulation in higher plants. Annual Review of Cell and Developmental Biology,1984,35:155-189.
247.Yi N, Kim YS, Jeong MH, Oh SJ, Jeong JS, Park SH, Jung H, Choi YD, Kim JK. Functional analysis of six drought-inducible promoters in transgenic rice plants throughout all stages of plant growth. Planta,2010,232:743-754.
248.Yin XR, Chen KS, Allan AC, Wu RM, Zhang B, Lallu N, Ferguson IB, Ethylene-induced modulation of genes associated with the ethylene signaling pathway in ripening kiwifruit. Journal of Experimental Botany,2008,59:2097-2108.
249.Yu SM, Ko SS, Hong CY, Sun HJ, Hsing YI, Tong CG, Ho THD. Global functional analyses of rice promoters by genomics approaches. Plant Molecular Biology,2007, 65:417-425.
250.Yuan S, Dean JFD. Differential responses of the promoters from nearly identical paralogs of loblolly pine (Pinus taeda L.) ACC oxidase to biotic and abiotic stresses in transgenic Arabidopsis thaliana. Planta,2010,232:873-886.
251.Zacchini M, de Agazio M. Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment. Plant Physiology and Biochemistry,2004,42:445-450.
252.Zarei A, Korbes AP, Younessi P, Montiel G, Champion A, Memelink J. Two GCC boxes and AP2/ERF-domain transcription factor ORA59 in jasmonate/ethylene-mediated activation of the PDF 1.2 promoter in Arabidopsis. Plant Molecular Biology,2011,75:321-331.
253.Zhang JS, Xie C, Shen YG, Chen SY. A two-component gene (NTHK1) encoding a putative ethylene-receptor homolog is both developmentally and stress regulated in tobacco. Theoretical and Applied Genetics,2001,102:815-824.
254.Zhao FG, Qin P. Protective effect of exogenous polyamines on root tonoplast function against salt stress in barley seedlings. Plant Growth Regulation,2004, 42:97-103.
255.Zheng H, Lin S, Zhang Q, Lei Y, Zhang Z. Function analysis of 5'untranslated region of a TIR-NBS-encoding gene from triploid white polar. Molecular Genetics and Genomics,2009,282:381-394.
256.Zhong S, Lin Z, Grierson D. Tomato ethylene receptor interaction:visualization of NEVER-RIPE interaction with multiple CTRs at the endoplasmic reticulum. Journal of Experimental Botany,2008,59:965-972.
257.Zhou HW, Dong L, Arie RB, Lurie S. The role of ethylene in the prevention of chilling injury in nectarines. Journal of Plant Physiology,2001,158:55-61.
258.Ziosi V, Scaramagli S, Bregoli AM, Biondi S, Torrigiani P. Peach (Prunus persica L.) fruit growth and ripening:transcript levels and activity of polyamine biosynthetic enzymes in the mesocarp. Journal of Plant Physiology,2003,160:1109-1115.
259.Zou TT, Rao JN, Liu L, Xiao L, Yu TX, Jiang P, Gorospe M, Wang JY. Polyamines Regulate the Stability of JunD mRNA by Modulating the Competitive Binding of Its 3' Untranslated Region to HuR and AUF1. Molecular and Cellular Biology,2010, 30:5021-5032.