小报春生物学特性与多倍体种质创新研究
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摘要
本研究以我国特有种小报春(Primula forbesii)为试验材料,系统研究其生物学特性;对小报春、胭脂花(Primula maximowiczii)、齿叶灯台报春(Primula serratifolia)、藏报春(Primula sinensis)、偏花报春(Primula secundiflora Franch)5种报春花属植物进行染色体核型分析;建立小报春组培快繁体系,并利用秋水仙素对小报春进行多倍体诱导,采用形态学、解剖学、细胞学、DNA含量、叶绿素荧光分析等途径进行多倍体鉴定,以诱导出多倍体小报春。对充分发挥和利用我国野生植物资源优势,保护、开发我国栽培名花和丰富园林花卉种类有重要意义。主要结果如下:
1通过对小报春生物学特性研究结果表明:种子的最适宜储存方式为低温干燥储藏,室温储藏1a后多数种子失去生命力。赤霉素对提高种子萌芽率作用不大,但经赤霉素处理的种子发芽时间短,发芽整齐。种子适宜发芽温度为20~25℃。小报春喜弱酸性水分和土壤环境,土壤和水质酸碱度(pH=6.8),利于种子萌发。以腐殖土∶泥炭藓∶细沙=2∶1∶1作为培养基质有利于种子萌发。小报春的花为异型花,是典型的异花授粉植物,花期在云南原生地为11月15日至3月26日,夜间无开花。晴天时小报春日开花高峰期均在11:15时～11:45时之间。阴天时小报春的花时较晴天时的花时更分散,而且开花高峰期向后延迟。小报春花粉属于3-拟孔沟花粉。长、短花柱花的花粉粒大小差异较大,短花柱的花粉粒较大,而长花柱的花粉粒较小,长柱型花有较长的花柱和乳突细胞,长花柱乳突约是短花柱的1.5倍,长花柱细胞的长度明显长于短花柱的细胞。长花柱花(♀)×短花柱花(♂)杂交组合或短花柱(♀)×长花柱花(♂)杂交组合均有较高的杂交结实率,长花柱花自交结实率为0。
2 5种报春花属植物染色体核型研究结果表明:小报春染色体核型公式:K(2n)=2x=18m,臂指数51.65%,核型为“1A”型。偏花报春染色体核型公式:K(2n)=2x=20m+2sm,臂指数52.00%,核型为“1A”型。藏报春染色体核型公式:K(2n)=2x=20m+2M,臂指数53.42%,核型属“1B”型。胭脂花染色体核型公式:K(2n)=2x=22m,臂指数51.07%,核型属于“1A”型。齿叶灯台报春染色体核型公式:K(2n)=2x=22m,臂指数52.59%,核型属于“1A”型。
3小报春组培快繁再生体系与多倍体诱导体系建立研究结果表明:用叶片作为外植体诱导愈伤组织形成快,质地紧密。激素浓度配比以NAA浓度为0.5mg·L~(-1),6-BA浓度为3.0mg·L~(-1)配比时愈伤组织诱导率最高,为85.0%,并且愈伤组织形成较快,质地紧密呈嫩绿色,具有分化能力。最适合小报春丛生芽诱导的培养基激素配方为MS+NAA0.10mg·L~(-1)+6-BA1.0mg·L~(-1）(pH=5.8)。以1/2MS为基本培养基,NAA浓度为1.00mg·L~（-1)时能在最短的时间培育出最多的根,生根率为80.0%,根粗壮。
利用秋水仙素处理组织培养过程中产生的丛生芽是诱导多倍体的有效方法。用0.10g·L~(-1)的秋水仙素处理小报春丛生芽48h,诱导效果最好,死亡率为15.0%,变异率为70.0%,为最佳处理组合。对变异植株进行细胞染色体鉴定最终的到了同源四倍体株系19株,其中13株来自秋水仙素对丛生芽诱导,6株是来源于秋水仙素对愈伤组织诱导而得到的。秋水仙素结合组织培养技术进行多倍体诱导获得多倍体植株的机率要比田间活体诱导效果显著。田间诱导没有得到四倍体植株。
4二倍体与四倍体小报春形态及细胞学研究结果表明:四倍体小报春比二倍体株高变矮,叶柄变短变粗,叶片明显变宽、变厚、颜色深绿,叶片背面的绒毛变多变长。四倍体植株单花冠直径明显增大,花的萼片、黄色的花眼都较对照增大。果实较对照增大,差异极显著。四倍体植株的气孔保卫细胞长度和宽度明显大于二倍体,气孔、保卫细胞的大小和叶绿体数与倍性成显著正相关性。四倍体小报春体细胞中期染色体由36条中部着丝点染色体组成。臂指数为(51.32±0.76)%,核型公式为K(2n)=4x=36m,核型不对称性属1A型。采用流式细胞仪和PartecDPAC软件对二倍体和部分经染色体数鉴定2n=4x=36的植株进行了细胞DNA含量测定和分析,结果表明,染色体加倍的植株其叶片细胞中的DNA含量也相应增加了一倍。与染色体数的鉴定结果一致。
5二倍体与四倍体小报春叶绿素荧光特性研究结果表明:四倍体小报春的叶绿素a、叶绿素b、叶绿素总含量及类胡萝卜素都高于二倍体小报春。在二倍体和四倍体中,四倍体的Fo小于二倍体,Fm、Fv、Fv/Fm、Fv/Fo和PI均大于二倍体。说明四倍体表现出较好的光合性能。
This study uses P. forbesii, which is endemic to China as test materials, and holds a systematic research of its characteristics of introducing cultivation, and its biological characteristics of flowing, pollination, fruiting. And the research also analysis the karyotype of five primroses genera plants P.forbesii, P.maximowiczii, P.serratifolia, P.sinensis, P.secundiflora Franch.Establish the rapid propagation system of the group growing P.forbesii and use the colchicine to polyploidy induce the P.forbesii. Using morphological and anatomical,cytology,DNA content,chlorophyll fluorescence analysis and such kind of means to conduct polyploid appraisal the purpose is to induce a polyploid P. forbesii to improve its appreciation,enhance its resistance.With its domesticated success and cultivating new polyploid primroses varieties, to make full use of our country’s wild resources advantage, and have important meanings in the protection of resources, develop cultivation in China. Main results are as follows:
1、The results of the Biological characteristics of P forbesii study indicate that: The fruit of P.forbesii is capsule, spherical, after dehydration the epicarp will craze membranously when mature, outside have 1-nerved calyx. Normally mature seeds are tiny, irregular hexahedral, their epidermis have mesh shape structure. The seeds of 1000-grain weight (0.118±0.0023) g. The seeds of the P.forbesii should be stored for dry at low temperature, if storage at the room temperature, after a year most seeds lose their vitality. GA3 in improving seed germination rate effect is not big, but the GA3 treatment of P.forbesii seed germination time is short, sprout neatly. The seeds of P.forbesii is suitable to make a spring at a germination temperature 20 ~ 25℃. P.forbesii is lean to weakly acidic water and soil environment. So in domesticated P.forbesii, the soil and the water should be improved to pH = 6.8 in order to favor the seed germination, make it normally grow up. In A. budding soil corrosion: peat moss: sand = 2:1:1 as train matrix helpful for P.forbesii’s seed germination, germinating capacities and sprout potential is obviously higher than other matrix. So in planting early introduction cultivation matrix should choose (budding soil corrosion: peat moss: sand = 2:1:1) as reproductive growth of soil matrix.
P.forbesii are typical different flowers pollination plants.The bloom days of P.forbesii in Yunnan are from around November 15 to March 26, without night blossom. During Sunny days, the blossom peak ranges between 11:15~11:45. During the Cloudy days, the blossom hours are more scattered than during the sunny days, and flowering peak backward delayed. Through the determination of the pollen life force of P.forbesii, the pollen could be cultivated in 25℃, which has the highest rate, within 30min have pollen germination, cultivate 120min after, pollen germination rate can reach 48.92%, after 180min observation shows pollen germination rate can reach 62.87%, the influence of temperature and sucrose for P.forbesii pollen germination shows that the optimum temperature conditions is 25℃, sucrose concentration of 10%.When boric acid concentration is 10mg/L, P.forbesii pollen germination rate is the highest. P.forbesii has the best storage result when stored in low-temperature -20℃add desiccant. From the body pollen germination method is suitable for the life force test of P.forbesii. P.forbesii belongs to 3-fitting hole groove pollen. The long and short flower of P.forbesii has very different the pollen grains sizes, short styles of the pollen grains is larger, but long styles of the pollen grains is lesser, long styles have longer stigma and papillary cells, long styles papilloma covenant is of 1.5 times of the length of short styles, the long styles cells obviously longer than short styles cells. The Hybrid seed setting of Long styles flowers (♀)×short styles flowers (♂) or short styles (♀)×long styles flowers (♂) combinations are higher.
2、The study of 5 kinds of primroses genus karyotype indicates: P.forbesii,Somatic chromosome number is 2n=2x=18, Brachial index is 51.65%, Karyotype formula is K(2n)=2x=18m, The karyotype asymmetry genera 1A. Chromosome of P.secundiflora Franch is larger, Centromere is more clear. Somatic chromosome number is 2n=2x=22, Brachial index is 52.00%, Karyotype formula is K(2n)=2x=20m+2sm, The karyotype asymmetry genera 1A. P.sinensis Somatic chromosome number is 2n=2x=22, Brachial index is 53.42%, Karyotype formula is K(2n)=2x=20m+2M, The karyotype asymmetry genera 1B. P.maximowiczii,Somatic chromosome number is 2n=2x=22, Brachial index is 51.07%, Karyotype formula is K(2n)=2x=22m, The karyotype asymmetry genera 1A.P.serratifolia Brachial index is 52.59%, Karyotype formula is K(2n)=2x=22m, The karyotype asymmetry genera 1A. except the P.secundiflora Franch, the karyotypes of other four plants are first reported.
3、The result of the building of P.forbesii tissue regeneration system and rapid propagation autopolyploid induction system shows that use blades of callus induction formed fast, texture closely. Alexipharmic disinfection mode selection method of dealing with Numbers is 8, namely: detergent cleaning 20min, tap water flushing 30min, ShengGong soak, 7min 0.1% sterile water flushing 4~6 times. Hormone concentration ratio in olumes is 0.5 mg·L-1, 6-BA concentration ratio is 3.0 mg·L-1 when callus induction 85.0%, and the highest for the callus forms faster, texture closely in peak green, which has differentiated ability. The most suitable hormone formula of multiple shoot clumps induction medium for P.forbesii is MS + NAA0.10 mg·L-1 + 6-BA1.0 mg·L-1 (pH = 5.8). With 1/2MS for basic medium, NAA concentration would be 1.00 mg·L-1, which can produced the most root in the shortest possible time, rooting rate is 80.0%, and roots are very strong.
It is an effective method in induction of polyploidy ornamental plants to use colchicine processing the clump sprouts during the process of tissue culture produces.With the 0.10% colchicine to process the clump sprouts of P. forbesiifor 48 h, which has the best inducing effect, the mortality rate is 15.0%, mutation rate is 70.0%, which is the best treatment combinations. Cell chromosome identification of the variant plants eventually results in strains of autotetraploid strains, with 13 strains from colchicine on clumps shoot induction and 6 strains are from colchicine on callus induction. The combination of colchicine and tissue growing skills will get much more polyploidy plants than in the field when using the polyploidy skills. In the field, there could not have induced tetraploid plants
4、The study of morphology and cytology on P.forbesiidiploid and tetraploid shows that: tetraploid P.forbesii’s heights are more shorter, petioles are more shorter and thicker, leaves are more wide and thick, its color is dark green, and more longer hair in the back of the leaves than diploid P.forbesii. Tetraploid plants, the average leaf index is 1.09±0.07 in contrast with 1.38±0.24 of the diploid average leaf index, tetraploid single corolla diameter increased significantly with an average of (2.27±0.03)cm, in contrast with(1.24±0.55)cm of the average corolla diameter, and the flower sepals, Yellow eye increased as compared with the control. The fruits are Larger than the ones in control, the fruit of tetraploid has an average diameter of(0.89±0.07)cm, whereas the fruit diploid has an average diameter of(0.41±0.15)cm, the difference was extremely significant. Stomatal guard cells of tetraploid plants were significantly greater than the diploid in the length and width, diploid guard cell chloroplasts is an average of 18, the average number of tetraploid guard cell chloroplasts is 55, reached a significant difference. Stomatal, Guard cell size and chloroplast’s number and ploidy is a significant positive correlation. Diploid stomatal density is(94.20±8.56)/mm2, tetraploid stomatal density is(78.91±6.97)/mm2, the stomatal density on the existence of diploid and tetraploid are significantly different. By chromosome analysis of colchicine processed cell regeneration plants, we found that some of the regenerated plants are tetraploid (2n = 4x = 36). By karyotype analysis, tetraploid somatic of metaphase chromosomes consist of 36 metacentric Centromere chromosomes. Brachial index is (51.32±0.76)%, karyotype formula is K (2n) = 4x = 36m, the karyotype asymmetry is 1A. To further determine the doubling plants homozygous of ploidy, using flow cytometry and PartecDPAC to conduct diploid and part of plants which were chromosomes identified 2n = 4x = 36, measured and analyzed cellular DNA content. he results show that the leaves of the chromosome doubling plant, whose DNA content in cells in a corresponding increase doubled, which is consistent with the identification chromosome numbers.
5、The preliminary study results of the chlorophyll fluorescence of P.forbesii diploid and tetraploid show that: Tetraploid P.forbesii’s chlorophyll a, chlorophyll b, total chlorophyll and carotenoids are higher than diploid’s. In diploid and tetraploid, the tetraploid’s Fo is smaller than diploid, but Fm, Fv, Fv / Fm, Fv / Fo and PI is higher than diploid, which indicates that tetraploid have better photosynthetic performance.
1通过对小报春生物学特性研究结果表明:种子的最适宜储存方式为低温干燥储藏,室温储藏1a后多数种子失去生命力。赤霉素对提高种子萌芽率作用不大,但经赤霉素处理的种子发芽时间短,发芽整齐。种子适宜发芽温度为20~25℃。小报春喜弱酸性水分和土壤环境,土壤和水质酸碱度(pH=6.8),利于种子萌发。以腐殖土∶泥炭藓∶细沙=2∶1∶1作为培养基质有利于种子萌发。小报春的花为异型花,是典型的异花授粉植物,花期在云南原生地为11月15日至3月26日,夜间无开花。晴天时小报春日开花高峰期均在11:15时～11:45时之间。阴天时小报春的花时较晴天时的花时更分散,而且开花高峰期向后延迟。小报春花粉属于3-拟孔沟花粉。长、短花柱花的花粉粒大小差异较大,短花柱的花粉粒较大,而长花柱的花粉粒较小,长柱型花有较长的花柱和乳突细胞,长花柱乳突约是短花柱的1.5倍,长花柱细胞的长度明显长于短花柱的细胞。长花柱花(♀)×短花柱花(♂)杂交组合或短花柱(♀)×长花柱花(♂)杂交组合均有较高的杂交结实率,长花柱花自交结实率为0。
2 5种报春花属植物染色体核型研究结果表明:小报春染色体核型公式:K(2n)=2x=18m,臂指数51.65%,核型为“1A”型。偏花报春染色体核型公式:K(2n)=2x=20m+2sm,臂指数52.00%,核型为“1A”型。藏报春染色体核型公式:K(2n)=2x=20m+2M,臂指数53.42%,核型属“1B”型。胭脂花染色体核型公式:K(2n)=2x=22m,臂指数51.07%,核型属于“1A”型。齿叶灯台报春染色体核型公式:K(2n)=2x=22m,臂指数52.59%,核型属于“1A”型。
3小报春组培快繁再生体系与多倍体诱导体系建立研究结果表明:用叶片作为外植体诱导愈伤组织形成快,质地紧密。激素浓度配比以NAA浓度为0.5mg·L~(-1),6-BA浓度为3.0mg·L~(-1)配比时愈伤组织诱导率最高,为85.0%,并且愈伤组织形成较快,质地紧密呈嫩绿色,具有分化能力。最适合小报春丛生芽诱导的培养基激素配方为MS+NAA0.10mg·L~(-1)+6-BA1.0mg·L~(-1）(pH=5.8)。以1/2MS为基本培养基,NAA浓度为1.00mg·L~（-1)时能在最短的时间培育出最多的根,生根率为80.0%,根粗壮。
利用秋水仙素处理组织培养过程中产生的丛生芽是诱导多倍体的有效方法。用0.10g·L~(-1)的秋水仙素处理小报春丛生芽48h,诱导效果最好,死亡率为15.0%,变异率为70.0%,为最佳处理组合。对变异植株进行细胞染色体鉴定最终的到了同源四倍体株系19株,其中13株来自秋水仙素对丛生芽诱导,6株是来源于秋水仙素对愈伤组织诱导而得到的。秋水仙素结合组织培养技术进行多倍体诱导获得多倍体植株的机率要比田间活体诱导效果显著。田间诱导没有得到四倍体植株。
4二倍体与四倍体小报春形态及细胞学研究结果表明:四倍体小报春比二倍体株高变矮,叶柄变短变粗,叶片明显变宽、变厚、颜色深绿,叶片背面的绒毛变多变长。四倍体植株单花冠直径明显增大,花的萼片、黄色的花眼都较对照增大。果实较对照增大,差异极显著。四倍体植株的气孔保卫细胞长度和宽度明显大于二倍体,气孔、保卫细胞的大小和叶绿体数与倍性成显著正相关性。四倍体小报春体细胞中期染色体由36条中部着丝点染色体组成。臂指数为(51.32±0.76)%,核型公式为K(2n)=4x=36m,核型不对称性属1A型。采用流式细胞仪和PartecDPAC软件对二倍体和部分经染色体数鉴定2n=4x=36的植株进行了细胞DNA含量测定和分析,结果表明,染色体加倍的植株其叶片细胞中的DNA含量也相应增加了一倍。与染色体数的鉴定结果一致。
5二倍体与四倍体小报春叶绿素荧光特性研究结果表明:四倍体小报春的叶绿素a、叶绿素b、叶绿素总含量及类胡萝卜素都高于二倍体小报春。在二倍体和四倍体中,四倍体的Fo小于二倍体,Fm、Fv、Fv/Fm、Fv/Fo和PI均大于二倍体。说明四倍体表现出较好的光合性能。
This study uses P. forbesii, which is endemic to China as test materials, and holds a systematic research of its characteristics of introducing cultivation, and its biological characteristics of flowing, pollination, fruiting. And the research also analysis the karyotype of five primroses genera plants P.forbesii, P.maximowiczii, P.serratifolia, P.sinensis, P.secundiflora Franch.Establish the rapid propagation system of the group growing P.forbesii and use the colchicine to polyploidy induce the P.forbesii. Using morphological and anatomical,cytology,DNA content,chlorophyll fluorescence analysis and such kind of means to conduct polyploid appraisal the purpose is to induce a polyploid P. forbesii to improve its appreciation,enhance its resistance.With its domesticated success and cultivating new polyploid primroses varieties, to make full use of our country’s wild resources advantage, and have important meanings in the protection of resources, develop cultivation in China. Main results are as follows:
1、The results of the Biological characteristics of P forbesii study indicate that: The fruit of P.forbesii is capsule, spherical, after dehydration the epicarp will craze membranously when mature, outside have 1-nerved calyx. Normally mature seeds are tiny, irregular hexahedral, their epidermis have mesh shape structure. The seeds of 1000-grain weight (0.118±0.0023) g. The seeds of the P.forbesii should be stored for dry at low temperature, if storage at the room temperature, after a year most seeds lose their vitality. GA3 in improving seed germination rate effect is not big, but the GA3 treatment of P.forbesii seed germination time is short, sprout neatly. The seeds of P.forbesii is suitable to make a spring at a germination temperature 20 ~ 25℃. P.forbesii is lean to weakly acidic water and soil environment. So in domesticated P.forbesii, the soil and the water should be improved to pH = 6.8 in order to favor the seed germination, make it normally grow up. In A. budding soil corrosion: peat moss: sand = 2:1:1 as train matrix helpful for P.forbesii’s seed germination, germinating capacities and sprout potential is obviously higher than other matrix. So in planting early introduction cultivation matrix should choose (budding soil corrosion: peat moss: sand = 2:1:1) as reproductive growth of soil matrix.
P.forbesii are typical different flowers pollination plants.The bloom days of P.forbesii in Yunnan are from around November 15 to March 26, without night blossom. During Sunny days, the blossom peak ranges between 11:15~11:45. During the Cloudy days, the blossom hours are more scattered than during the sunny days, and flowering peak backward delayed. Through the determination of the pollen life force of P.forbesii, the pollen could be cultivated in 25℃, which has the highest rate, within 30min have pollen germination, cultivate 120min after, pollen germination rate can reach 48.92%, after 180min observation shows pollen germination rate can reach 62.87%, the influence of temperature and sucrose for P.forbesii pollen germination shows that the optimum temperature conditions is 25℃, sucrose concentration of 10%.When boric acid concentration is 10mg/L, P.forbesii pollen germination rate is the highest. P.forbesii has the best storage result when stored in low-temperature -20℃add desiccant. From the body pollen germination method is suitable for the life force test of P.forbesii. P.forbesii belongs to 3-fitting hole groove pollen. The long and short flower of P.forbesii has very different the pollen grains sizes, short styles of the pollen grains is larger, but long styles of the pollen grains is lesser, long styles have longer stigma and papillary cells, long styles papilloma covenant is of 1.5 times of the length of short styles, the long styles cells obviously longer than short styles cells. The Hybrid seed setting of Long styles flowers (♀)×short styles flowers (♂) or short styles (♀)×long styles flowers (♂) combinations are higher.
2、The study of 5 kinds of primroses genus karyotype indicates: P.forbesii,Somatic chromosome number is 2n=2x=18, Brachial index is 51.65%, Karyotype formula is K(2n)=2x=18m, The karyotype asymmetry genera 1A. Chromosome of P.secundiflora Franch is larger, Centromere is more clear. Somatic chromosome number is 2n=2x=22, Brachial index is 52.00%, Karyotype formula is K(2n)=2x=20m+2sm, The karyotype asymmetry genera 1A. P.sinensis Somatic chromosome number is 2n=2x=22, Brachial index is 53.42%, Karyotype formula is K(2n)=2x=20m+2M, The karyotype asymmetry genera 1B. P.maximowiczii,Somatic chromosome number is 2n=2x=22, Brachial index is 51.07%, Karyotype formula is K(2n)=2x=22m, The karyotype asymmetry genera 1A.P.serratifolia Brachial index is 52.59%, Karyotype formula is K(2n)=2x=22m, The karyotype asymmetry genera 1A. except the P.secundiflora Franch, the karyotypes of other four plants are first reported.
3、The result of the building of P.forbesii tissue regeneration system and rapid propagation autopolyploid induction system shows that use blades of callus induction formed fast, texture closely. Alexipharmic disinfection mode selection method of dealing with Numbers is 8, namely: detergent cleaning 20min, tap water flushing 30min, ShengGong soak, 7min 0.1% sterile water flushing 4~6 times. Hormone concentration ratio in olumes is 0.5 mg·L-1, 6-BA concentration ratio is 3.0 mg·L-1 when callus induction 85.0%, and the highest for the callus forms faster, texture closely in peak green, which has differentiated ability. The most suitable hormone formula of multiple shoot clumps induction medium for P.forbesii is MS + NAA0.10 mg·L-1 + 6-BA1.0 mg·L-1 (pH = 5.8). With 1/2MS for basic medium, NAA concentration would be 1.00 mg·L-1, which can produced the most root in the shortest possible time, rooting rate is 80.0%, and roots are very strong.
It is an effective method in induction of polyploidy ornamental plants to use colchicine processing the clump sprouts during the process of tissue culture produces.With the 0.10% colchicine to process the clump sprouts of P. forbesiifor 48 h, which has the best inducing effect, the mortality rate is 15.0%, mutation rate is 70.0%, which is the best treatment combinations. Cell chromosome identification of the variant plants eventually results in strains of autotetraploid strains, with 13 strains from colchicine on clumps shoot induction and 6 strains are from colchicine on callus induction. The combination of colchicine and tissue growing skills will get much more polyploidy plants than in the field when using the polyploidy skills. In the field, there could not have induced tetraploid plants
4、The study of morphology and cytology on P.forbesiidiploid and tetraploid shows that: tetraploid P.forbesii’s heights are more shorter, petioles are more shorter and thicker, leaves are more wide and thick, its color is dark green, and more longer hair in the back of the leaves than diploid P.forbesii. Tetraploid plants, the average leaf index is 1.09±0.07 in contrast with 1.38±0.24 of the diploid average leaf index, tetraploid single corolla diameter increased significantly with an average of (2.27±0.03)cm, in contrast with(1.24±0.55)cm of the average corolla diameter, and the flower sepals, Yellow eye increased as compared with the control. The fruits are Larger than the ones in control, the fruit of tetraploid has an average diameter of(0.89±0.07)cm, whereas the fruit diploid has an average diameter of(0.41±0.15)cm, the difference was extremely significant. Stomatal guard cells of tetraploid plants were significantly greater than the diploid in the length and width, diploid guard cell chloroplasts is an average of 18, the average number of tetraploid guard cell chloroplasts is 55, reached a significant difference. Stomatal, Guard cell size and chloroplast’s number and ploidy is a significant positive correlation. Diploid stomatal density is(94.20±8.56)/mm2, tetraploid stomatal density is(78.91±6.97)/mm2, the stomatal density on the existence of diploid and tetraploid are significantly different. By chromosome analysis of colchicine processed cell regeneration plants, we found that some of the regenerated plants are tetraploid (2n = 4x = 36). By karyotype analysis, tetraploid somatic of metaphase chromosomes consist of 36 metacentric Centromere chromosomes. Brachial index is (51.32±0.76)%, karyotype formula is K (2n) = 4x = 36m, the karyotype asymmetry is 1A. To further determine the doubling plants homozygous of ploidy, using flow cytometry and PartecDPAC to conduct diploid and part of plants which were chromosomes identified 2n = 4x = 36, measured and analyzed cellular DNA content. he results show that the leaves of the chromosome doubling plant, whose DNA content in cells in a corresponding increase doubled, which is consistent with the identification chromosome numbers.
5、The preliminary study results of the chlorophyll fluorescence of P.forbesii diploid and tetraploid show that: Tetraploid P.forbesii’s chlorophyll a, chlorophyll b, total chlorophyll and carotenoids are higher than diploid’s. In diploid and tetraploid, the tetraploid’s Fo is smaller than diploid, but Fm, Fv, Fv / Fm, Fv / Fo and PI is higher than diploid, which indicates that tetraploid have better photosynthetic performance.
引文
[1]中国科学院中国植物志编委会.中国植物志(第59卷第2分册)[M].北京:科学出版社,1990.
[2]Richards J.Primula[M].London:B. T. Batsford Ltd.1993.
[3]贾茵,张启翔.60Coγ-射线辐射对小报春种子萌发和M1性状的影响[J].种子,2009,28(11):1-3.
[4]李世峰,李伟琼.云南报春花种质资源分布特点及观赏类型[J].江西农业学报,2010,22(3): 85-87.
[5]赵艳昆,过永慧.昆明地区小报春的栽培[J].林业调查规划,2004(增刊):291-292.
[6]邓平平.六种报春花属植物的组织培养与多倍体诱导研究[D].北京林业大学,硕士论文,2007.
[7]梁树乐.我国西南地区部分报春花的引种与杂交育种研究[D].北京林业大学,博士论文,2006.
[8]胡伟娟,张启翔,潘会堂.报春叶片解剖结构与耐热性的关系[J].华中农业大学学报,2010,293(3):363-369.
[9]陈封怀,胡启明.中国报春花科植物系统分类研究.中国科学院院刊,1996(6):445~446.
[10]陈俊愉.国内外花卉科学研究与生产开发的现状与展望.中国园艺学会第八届年会上的报告,1997.
[11]李林光.苹果多倍体种质创新及鉴定评价研究[D].沈阳农业大学,博士论文,2008.
[12]刘小莉.报春花遗传变异的初步研究[D].云南大学,硕士论文,2004.
[13]刘飞虎,梁雪妮等.4种野生报春花光合特征的比较[J].园艺学报,2004,31(4):482-486.
[14]朱惠芬.五种观赏报春引种驯化的初步研究[D].中科院昆明植物研究所,硕士论文,2001.
[15]朱惠芬.九种报春花植物的核形态学研究[J].云南植物研究,2001,23(4):466-472.
[16] DafniA, HesseM,PaciniE. Polen and pollination[M]. New York:Springer-Verlag,2000,9:111–126.
[17]许玲.兰草再生与转化体系的建立及其离体诱变种质创新[D].浙江大学.博士论文,2009.
[18]贾文庆,刘会超,姚连芳.3个东方百合品种生物学特性观察及杂交亲和性研究[J].西北林学院学报,2007,22(6):18-20.
[19]刘海萍.药用植物茅苍术的组织培养[J].现代中药研究与实践,2005,19(5):10-12.
[20]Erica E.Benson,J.E.Danaher,In vitro micropropagation of Primula scotica:a rare Scottish plant[J],Biodiversity and Conservation 2000,9:711–726.
[21]娄和林.不同pH值对樱草原生质体分离的影响[J].植物学通报,1990,7(4):38-39.
[22]娄和林.四季樱草叶肉原生质体植株再生[J].辽宁大学学报,2005,9(5):19-21.
[23]程金水.园林植物遗传育种学[M].北京:中国林业出版社,2000.
[24]Coumans M,Gaspar Th.Mass propagation of Primula obconica[J].Acta Horticulturae.1979, 91:287-289.
[25]Margaret A,Webster P M.Acomparsion of early floral ontogeny in wild-type and floral homeotic mutant phenotypes of primula[J].Planta.2003,216:903-907.
[26]Kato J,Mii M.Differences in level of inter-specific hybrids obtained by reciprocal crosses between Primula seiboddii and Primula kisoana[J].Theor Appl Genet,2000,101:690-696.
[27]Karlsson M G.Primula culture and production[J].Hort Technology,2001,11(4):627-635.
[28]Yamamoto T.Mass propagation of Primula sieboldii E.Morr.through leat segment culture.Bulletianof the Faculty of Horticulture Minamikyushu University-Series [J].Natural Science.1999,29:9-14.
[29]Mizuhiro M.Prodction and characterization of interspecific somatic hybrids between Primula malacoides and P.obconica[J].Plant Sci.2001,161:489-496.
[30]Bajaj Y P S.Regeneration of plants from ultra-low frozen anthers of Primula obconica[J].Scientia Horti.1981,14(1):93-95.
[31]刘雪微.矮牵牛组织培养繁殖技术研究[J].现代园艺,2008,21(10):57-58.
[32]严春艳,马伟丽,于荣敏.长春花愈伤组织诱导及培养条件优化[J].中药材,2008,31(7):955-956.
[33]李娜.蝴蝶兰的组织培养技术研究[J].江西农业学报,2008,20(9):51-53.
[34]谭文澄,戴策刚.观赏植物组织培养技术[M].北京:中国林业出版社,1991.141.
[35]周家琪.花卉学[M].北京:中国林业出版社,1988.204.
[36]侯云屏,古志渊.报春花的组织培养和快速繁殖[J].植物生理学通讯,2001,37(3):234-235.
[37]李俊明.植物组织培养教程[M].北京:中国农业大学出版社,1992.
[38]颜昌敬.植物组织培养手册[M].上海:上海科学技术出版社.1980.
[39]韦三立.花卉组织培养[M].北京:中国林业出版社,2001.
[40]张树军,李元,韩冰.矮牵牛的组织培养与快繁[J].内蒙古农业科技,2001,7(4):16-18.
[41]李士安.植物组培在花卉上的应用[J].植物生理学通讯, 1988,20(1):69-71.
[42]刘青林.花卉组织培养[M].北京:中国农业出版社,2006.
[43]巩振辉.花卉脱毒与快繁新技术[M].杨凌:西北农林科技大学出版社,2005.
[44]王家福.花卉组织培养与快繁技术[M].北京:中国林业出版社,2006.
[45]唐道城,梁顺祥.观赏植物组织培养研究进展[J].青海大学学报(自然科学版),2006,24(4):5-9.
[46]陈黎.观赏植物的组织培养繁殖技术研究综述[J].黄山学院学报,2005,7(3):55-57.
[47]刘忠荣,陈屏昭.植物组织培养技术在观赏植物中的应用[J].昭通师范高等专科学校学报,2003,25(5):41-43.
[48]博术琳,程智慧,徐重益.植物组织培养快繁体系与产业化[J].现代化农业,2002,21(4):4-5.
[49]李景秀,孔繁才.高穗报春的引种栽培[J].云南农业科技,2000,19(3).17-19.
[50]朱惠芬.五种观赏报春引种驯化的初步研究[D].中国科学院昆明植物研究所硕士学位论文,2001.
[51]薛大伟,张长芹,黄媛.云南无量山报春花种质资源的调查[J].园艺学报.2003,30(4):476-478.
[52]袁亚夫,潘开文.瓦屋山地区野生报春花资源及其开发利用[J].山地学报,2000,18(2):263~266.
[53]Bruun H G..The cytology of the genus Primula (a preliminary report)[J].Svensk Bot Tidskr, 1930,24(3):468-475.
[54]Bruun H G,.Cytological studies in Primula [J].Symb Bot Upsala.1932,1:1-239.
[55]Nakata M,Wu Q A,Kurokawa S.Cytological studies on Chinese plants introduced from Yunnan Province.I. 1997.
[56]李晓林.低温和植物生长调节剂对桃花粉萌发的影响[J].西南农业大学学报,2005,27(4):526-529.
[57]张长芹.五种野生观赏报春花引种驯化初报[J].云南植物研究,2003,25(2):216~222.
[58]朱慧芬,张长芹,顾志建.九种报春花属植物的核形态学研究[J].云南植物研究,2001,23(4):466-472.
[59]薛大伟,张长芹.云南无量山四种报春花属植物的核型分析[J].云南植物研究,2003,25(1):78~82.
[60]Dalgard V.Additional chromosome numbers in vascular plants from the Disko Bugt area (West Greenland) [J] Willenouia, 1989,19:199-213.
[61]Loon J C V,Josina J M H O.Chromosome numbers of some angiosperms of the southern U.S.S.R[J].Act Bot Neerl, 1976,25(5):329-336.
[62]郝瑞娟,穆鼎,张檀等.百合品种间杂交的初步研究[J].西北林学院学报,2005,20(3):87-89.
[63]裴新澍.多倍体诱导与育种[M].上海科学技术出版社,1963,95-104.
[64]王鸿鹤,葛欣.秋水仙素诱导重瓣大岩桐多倍体研究.热带亚热带植物学报,1999,7(3):237-242.
[65]Sakaya S R,Joshi K K.Karymorphological studies in some Primula species of Nepal Himalaya [J].Cytologia, 1990,55:571-579.
[66]Sarkar A K.Cytotaxonomic studies of some members of Primulaceae from the Eastern Himalaya[J].Proc Indian Sci Congr Assoc, 1973,60:316.
[67]王长泉,张文胜.苹果叶片离体培养中秋水仙素加倍效应的研究[J].果树科学,2005,16(2):104-109.
[68]王敏琴,王晓红.葡萄多倍体诱导的初步研究[J].山东农业科学,2000,12(1):19-22.
[69]杨帆.薄壳山核桃及云南山核桃染色体核型分析[J].江西林业科技,2001,5(2):6-7.
[70]韩毅科,杜胜利,王明等.黄瓜染色体加倍研究[J].天津农业科技,2002,16(8):1-4.
[71]杜仙明,郑素秋.小白菜多倍体诱导试验[J].湖南农学院学报,1995,21(1):25-27.
[72]郭启高,张钟灵,周红等.秋水仙碱诱导生姜多倍体的研究[J].园艺学报,2000,12(5):400-402.
[73]李雪斌.兰州百合多倍体试验报告[J].甘肃农业科技,1995,16(6):14-15.
[74]Sarkar A K.Primulaceae-its evolution and assessment in status as judged through cytotaxonoy [J].Feddes Report, 1988,99:113-132.
[75]Shrama A,Sarkar A K.Chromosome numbers in some higher plants [J].The Research Bulletin, 1967,2:38-48.
[76]Tanaka R.Recent karyotype studies .In Ogawa K,et al.(eds):Plant Cytology[M].Tokyo:Asakura Shoten,1977,3:293-326.
[77]Wendelbo P..Studies in Primulaceae .I.A monographjof the genus Dionysia [J] .Arab Univ Bergen,Mat.Nat,1961,(3):1-83.
[78]Smith W W,Forrest G.The sections of the genus Primula[J].Journal of the Royal Horticultural Society,1928,54:4-114.
[79]Tanaka R.Types of resting nuclei in Orchidaceae [J].Bot Mag Tokyo,1971,84:118-112.
[80]Wendelbo P.Studies in Primulaceae . II.An account of Primula subgenus Sphondylia with a review of the subdivisions of the genus[J].Arab Univ Bergen,Mat.Nat,1961(11):1-49.
[81]彭华.滇中南无量山种子植物[M].昆明:云南科技出版社,1998.1-170.
[82]任唯勇.花卉和观赏植物种子发芽方法[J].种子,2001,23(4):466-472.
[83]乔玉刚,宋芸.蔬菜多倍体育种研究综述[J].长江蔬菜,2000,5(1):8-10.
[84]胡启明.种子植物科属地理[M].北京:科学出版社,1999,331-343.
[85]李长海.濒危岩报春的引种栽培[J].东北林业大学学报,1994, 22(2):54-59.
[86]曹仪植,宋占午.植物生理学[M].兰州大学出版社,1998.1-259.
[87] Richards J.Primula[M].Poland,OR:Timber Press,1993:3-4.
[88]高俊平,姜伟贤.中国花卉科技二十年.科学出版社,2000.
[89]李洁,张少艾.萱草属若干野生种园艺品种染色体核型的比较研究[J].上海农学院学报,1995,13(3):208-217.
[90]Mizuhiro M,Yamada K.Plant regeneration from cell suspension culture-derived protoplasts of Primula malaciodes and P.obconica[J].Plant Sci.2001,160:1221-1228.
[91]陈学森,邓秀新,章文才等.中国银杏资源染色体数目及核型研究初报[J].华中农业大学学报,1996,25(6):590-593.
[92]黄少甫,赵治芬.三种观赏植物的染色体研究[J].植物研究,1995,15(1):43-46.
[93]武全安.云南珍稀濒危植物的研究[J].植物引种驯化集刊,1990,23(4):173-179.
[94]胡叔良.草本植物引种驯化某些规律的探讨[J].植物研究,1987,21(2):301-306.
[95]林夏珍,赵建强.中国野生花卉种质资源调查综述[J].浙江林学院学报,2001,18(4):441-444.
[96]徐振华.ABT生根粉提高报春花种子活力的研究[J].河北林果研究,1999,14(3):234-237.
[97]范眸天.云南野生花卉[J].云南科技出版社,2001,12(3):12-15.
[98]李长海.濒危岩报春的引种栽培[J].东北林业大学学报,2005,12(3):22-25.
[99]郑维列.西藏色季拉山报春花种质资源及其生境类型[J].园艺学报,1992,19(3):261~266.
[100]吴之坤,张长芹.滇西北玉龙雪山报春花种质资源的调查[J].广西植物,2006,19(1):19-21.
[101]陈明林.安徽羽叶报春和毛茛叶报春的物种生物学研究[D].硕士论文,2002.05.
[102]赖力.峨嵋山报春花属植物引种研究[J].植物引种驯化集刊,1995年第10集.
[103]娄和林.四季樱草叶肉原生质体植株再生[J].辽宁大学学报,2005,23(l):58-59.
[104]郭清泉,郑思乡,杨瑞芳等.莲多倍体诱导及变异植物形态特征和细胞学观察[J].湖南农业大学学报,1997,23(1):26-29.
[105]栾岚,佟凤芹.不同培养基对四季樱草叶再生植株形成的影响[J].辽宁大学学报,1995,(3):122-126.
[106]XingHW,LaiL study on seed generination of primula[J].Plant Introduction and Acclimatization. 2001,11(4):627-635.
[107]胡伟娟,张启翔,潘会堂.报春花属植物耐热性生理指标研究初探[J].中国农学通报,2010,26(5):158-163.
[108]张云中,伍正容.多花樱草(Primula×polyantha Hort)自花授粉与异型花间授粉的研究[J].植物学集刊,1992,5:222-227.
[109]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究,1985,3(4):297-301.
[110]李懋学.植物染色体研究技术[M].哈尔滨:东北林业大学出版社,1991,3(4):131-145.
[111]幸宏伟,赖力.报春花属植物种子萌发的研究[J].植物引种驯化集刊,1997年第11集.
[112]Chen J F,Staub J E,Jiang J.A reevaluation of karyotype in Cucumber [J].Genet Res CropEvol,1998,45,301-305.
[113]Jaffe M J,Galston A W. The physiology of tendrils [J].Annu Rev Physiol,1986,19:417-434.
[114]Yadav R C ,Grumet R.Tendrils as an alternate tissue source for chromosome visualization [J].Journal of the American Society for Horticultural Science,1994,119(4):850-852.
[115]王月霞,王苏玲.微型水仙染色体C—带带型研究[J].园艺学报,1996,21(3):274-276.
[116]Brandham P.E.,Kirton P.R..The chromosomes of species,hybrids and cultivars of Narcissus L[J].Amaryllidaceae Kew Bulletin,1987,42(1):23-29.
[117]Gill.B.S,Friebe B.and Endo T.R.Standard karyotype and nomenclature system for description of chromosomes bands and structural aberrations in heat[J].Genome, 1991,l(34):830-839.
[118]杨继.植物多倍体基因组的形成与进化[J].植物分类学报,2001,39(4):357-371.
[119]郭启高,宋明,梁国鲁.植物多倍体诱导育种研究进展[J].生物学通报,2000,35(2):8-11.
[120]Hansen N.J.P,et al.In vitro chromosome doubling potential of colchicines,oryzalin,trifluralin,and APM in Brasica napus microspore culture[J].Euphytica,1996,(88):159-164.
[121]Chen CH,Goeden-kallemeyn YC.In vitro induction of tetraploid plants from colchicines-treated diploid daylily callus[J].Euphytics,1979,(28):705-709.
[122]陈发棣,蒋甲福,房伟民.秋水仙素诱导菊花脑多倍体的研究[J].上海农业学报,2002,(18):46-50.
[123]牛维和,徐玉冰,刘纪红.诱导君子兰多倍体的初步研究[J].园艺学报,1986,13(1):61-63.
[124]岳烨.诱导金鱼草多倍体的初步研究[J].园艺学报,1990,17(1):76-80.
[125]唐岱,熊济华,王仕玉.切花菊育种问有探讨[J].云南农业大学学报,2001,16(1):45-49.
[126]陈俊愉,王四清.花卉育种中的几个关键环节[J].园艺学报,1995,9(4):372-376.
[127]费水章.切花菊再生植株形态和细胞学变异的研究[J].园艺学报,1994,12(2):193-198.
[128]韩礼星,赵改荣,李玉红等.猕猴桃多倍体诱导研究[J].果树科学,1998,15(3),273-276.
[129]郑思乡,李立良.芦笋多倍体诱导及其离体培养的研究[J].湖南农业科学,1986,21(1):22-23.
[130]Goldblatt,P.Index to plant chromosome numbers 1975-1978[J].Missouri Bot.Garden,1981,142.
[131]Lee,Y.N.Chromosome numbers of flowering plants in korea.J[J].Korea Res.Inst.Ewha Women’s Unive.1967.11:455-478.
[132]Stebbins,G.L.Chromosomal evolutiong in higher plants[J] Edward Arnold Ltd,London. 1971:85-104.
[133]涂淑萍,傅波,喻苏琴.星冠水仙引种栽培及生物学特性研究[J].江西农业大学学报,2001, 21(1):96-98.
[134]姚志芬.哈尔滨市几种宿根花卉得应用[J].东北林业大学学报,2000,12(2):31-34.
[135]Iles-JK,Agnew-NH. Seasonal cold-acclimation patterns of Sedum spectabile x telephium L.’Autum Joy’and Sedum spectabile Boreau .’Brillant’[J].Hort Science.1995,30(6):1221-122.
[136]Dexfer ,S.T Toffingham and L.T[J].Craber.Plant physiol ,1930,5:215-213.
[137]Warry MOA ,et al .Reproductive response of cowpea to heat stress[J] .Crop Sci,1983 (23 ):1088.
[138]Rajashekar C ,Westwood MN .Deep supercooling and cold hardiness in genus pyrus[J].J Amer Soc Hort Sci,1982 ,107(6 ):968-972.
[139]Tahir M, Signh M. Assessment of screening techniques for heat tolerance in wheat[J]. Crop Sci,1993,33:740-744.
[140]Arora-R, Pitchay-DS,Beac-BC. Water-stress-induced heat tolerance in geranium leaf tissues: a possible linkage through stress proteins[J]. Phsiologia-Plantarum,1998, 103:1,24-34.
[141]Marcum-KB. Cell membrane thermostability and whole-plant heat tolerance of Kentucky bluegrass[J]. Crop Science,1998,38:1214-1218.
[142]Ranney TG, Peet MM. Heattolerance of five taxa birch:physiological responses to supraoptimal leaf temperatures. J. Amer. Hort. Sci. ,1994,119:243-248.
[143]Ranney TG,Blazich FA,Warren. Heat tolerance of selected species and populations of Rhodo-dendron[J]. JAmer Hort Sci. ,1995,120:423-428.
[144]Bauer-H, Nagele-M, Comploj-M, Galler-V,Mair-M, Unterpertinger-E. Photosynthesis after freezing stress in plants with various degreess of freezing tolerance[J]. Photosynthetica,1992, 27: 627-635 .
[145]Vierling A, Nguyen HT. Heat-shock gene expression in diploid wheat genotypes differing in ther-mal tolerance[J]. Crop Sci. ,1992,32:370-377.
[146]Youngner VB,Nudge FJ. Growth and carbohydrate storage of three Poa pratensis L. strains asinfluenced by temperature[J]. Crop Science,1968,8:455-457.
[147]Watschke TL,Schmidt RE. Some metabolic phenomena of Kentucky Bluegrass under high temperature[J].Crop Science,1972,12:87-90.
[148]吴应祥.中国兰花.北京:中国林业出版社,1991.
[149]田梅生,王伏雄,钱南芬等.四季兰种子离体萌发及器官建成的研究[J].植物学报,1985,27(5):455-459.
[150]王熊,吴敦肃.建兰类原球茎生长、发育过程中扫描电镜观察[J].植物生理学报,1991,17(2):192-196.
[151]张菊野,俞琳凤,连宏乾等.几种影响春兰原球茎生长与分化的因素[J].植物生理学通讯,1993,29(3):175-178.
[152]何立珍,周朴华,刘选明.黄花菜同源四倍体与二倍体形态及细胞遗传学分析[J].湖南农学院学报,1994,20(4):332-337.
[153]韩志慧,孟广云,赵素君.凤仙组织培养快繁技术的研究[J].天津农学院学报,2001,8(3):31-33.
[154]曾前.新几内亚凤仙的组织培养和快速繁[J]殖.中国林副特产,2002,(2):12-14.
[155]张立磊,杨和连,李靓.新几内亚凤仙的组培快繁研究[J].河南职业技术师范学院学报,2003,31(3):21-23.
[156]刘玉芹,王震星,李树和等.新几内亚凤仙组培快繁技术研究[J].天津农学院学报,2003,10(1):11-13.
[157]Cox.E.H.M.&Tayor,G.C. Primulas for Garden and Greenhouse.A.R.Mowbray CoLondon. 1957.
[158]张淑娟.四季樱草的组织培养和快速繁殖[J].植物生理学通讯,2002,38(4):354.
[159]徐振华.ABT生根粉提高报春花种子活力的研究[J].河北林果研究,1999,14(3):234-237.
[160]苏文化,张光飞.土壤温度与气温对紫花雪山报春光合作用和蒸腾作用的影响[J].西北植物学报,2002,22(4):824-830.
[161]刘飞虎,梁雪妮.4种野生报春花光合特征的比较[J].园艺学报,2004,31(4):482-486.
[162]李涵,龙春林,郑思乡.沉香虎头兰多倍体诱导及其鉴定[J].园艺学报,2005,32(5):853.
[163]张志胜,黎扬辉,姜蕾.红掌四倍体的离体诱导及其鉴定[J].园艺学报,2007.34 (3):729-734.
[164]郑思乡.东方百合多倍体诱导及种球繁育的研究[J].西南农业大学学报,2004,34 (3):46-49.
[165]郑思乡.离体培养条件下三色堇多倍体诱导研究[J].云南林业科技,2003,31(4):482-486.
[166]WaichiroK. Mass in vitro production of bulblets of Lilium japonicum Houtt[J]. J. Japan Soc. Hort. Sci, 1993, 61:611-618.
[167]张兴翠.花叶绿萝的多倍体诱导及快速繁殖[J].西南农业大学学报,2003,27(5):455-459.
[168]连雪斌.兰州百合多倍体诱导试验报告[J].甘肃农业科技,1995,12(6):14-18.
[169]康向阳,朱之悌.白杨2n花粉生命力测定方法及萌发特征的研究[J].云南植物研究, 1997,19(4):402-406.
[170]SternR A S Gazit.Lychee pollination by the oncybee[J].J.Amer.Soc.Hort.Sci,1996,120(1):152-157.
[171]Chen J F,Staub J E,Jiang J.A reevaluation of karyotype in Cucumber [J].Genet Res Crop Evol,1998,45:301-305.
[172]Jaffe M J,Galston A W. The physiology of tendrils [J].Annu Rev Physiol,1986,19:417-434.
[173]Yadav R C ,Grumet R.Tendrils as an alternate tissue source for chromosome visualization [J].Journal of the American Society for Horticultural Science,1994,119(4):850-852.
[174]Brandham P.E.,Kirton P.R. The chromosomes of species,hybrids and cultivars of Narcissus L[J].Amaryllidaceae Kew Bulletin,1987,42(1):23-37.
[175]Gill.B.S,Friebe B.and Endo T.R..Standard karyotype and nomenclature system for description of chromosomes bands and structural aberrations in wheat[J].Genome,1991,34:830-839.
[176]Hansen N.J.P,et al.In vitro chromosome doubling potential of colchicines,oryzalin,trifluralin,and APM in Brasica napus microspore culture[J].Euphytica,1996,(88):159-164.
[177]Chen CH,Goeden-kallemeyn YC.In vitro induction of tetraploid plants from colchicines-treated diploid daylily callus[J].Euphytics,1979,(28):705-709.
[178]Goldblatt,P.Index to plant chromosome numbers 1975-1978.Missouri Bot.Garden,1981,142.
[179]Lee,Y.N.Chromosome numbers of flowering plants in korea.J.Korea Res.Inst.Ewha Women’s Unive,1967,11:455-478.
[180]Stebbins,G.L.Chromosomal evolutiong in higher plants. Edward Arnold Ltd,London. 1971:85-104.
[181]Ahloowalia BS,MaluszynskiM. Inducedmutations-A new paradigm in plantbreeding[J].Euphytica, 2001,18(2): 67-173.
[182]郭安熙,杨保安,范家霖等.金光四射等六个菊花新品种的辐射选育[J].核农学通报,1991,12(2):73-75.
[183]牛传堂,何道一,李雅志.辐射诱变梅花突变体的研究[J].核农学报,1995,9(3):144-148.
[184]陈秀兰,包建忠,刘春贵等.观赏荷花辐射诱变育种初报[J].核农学报,2004,18(3):201-203.
[185]吴光升,强继业,陈立.60Coγ-射线对美女樱、菠萝菊根长、芽长及出芽率的影响[ J].安徽农业科学, 2005, 33 (6):1 032-1 033.
[186]徐冠仁.植物诱变育种学[M].北京:中国农业出版社,1996:91.
[187]潘光辉,王文强,尹贤贵.电导法鉴定番茄耐热性与田间结果的比较[J]西南园艺,2001,21(2):26-27.
[188]张睿鹂.滇西北报春资源及滇北球花报春核心种质的研究[D].北京:北京林业大学,2008.
[189]陈立松,刘星辉.高温对桃和柚细胞膜透性和光合色素的影响[J].武汉植物学研究,1997,21(15):234-238.
[190]刘祖祺,张石城.植物抗性生理学[M].北京:中国农业出版社,1995:199-215.
[191]曾韶西,王以柔.低温下黄瓜幼苗子叶硫氢基(SH)含量变化与膜脂过氧化[J].植物学报,1991,33(1):50-54.
[192]王荣富.植物抗寒指标的种类及其应用[J].河北农业大学学报,1987,12(3):49-55.
[193]汤章城.植物抗逆性生理生化研究的某些进展[J].植物生理学通讯,1991,27(2):146-148.
[194]叶陈亮,柯玉陈,陈伟.大白菜耐热性的生理研究.叶片水分和蛋白质代谢与耐热性[J].福建农业大学学报,1996,9(25):490-493.
[195]孟焕文,张彦峰,程智慧.黄瓜幼苗对热胁迫的生理反应及耐热鉴定指标筛选[J].西北农业学报,2000,9(1):47-49.
[196]张伟艳,王玲,卓丽环等.东北3种野生鸢尾种子生物学比较研究[J].种子, 2006,25(12): 34-37.
[197]刘强,卓丽环,陈杰等.野鸢尾种子开花结实生物学特性[J].东北林业大学学报, 2005,33(1): 47-48.
[198]曲寿河,刘玉章,孙伟等.野鸢尾的栽培及利用[J].特种经济动植物, 2008(8):19-20.
[199]刘强.野鸢尾生物学特性的研究[D].东北林业大学硕士论文,2005,1-45.
[200]彭幼芬.种子生理学[M].湖南:中南工业大学出版社,1994:262-266.
[201]王桂芹.不同生态环境马蔺植物体解剖结构比较[J].内蒙古民族大学学报:自然科学版,2002, 17(2):127-129.
[202]颜启传.种子学[M].北京:中国农业出版社,2001:91-101.
[203]沈云光,管开云,王仲朗等.四种国产鸢尾属植物种子萌发特性研究[J].种子,2005,24(12): 21-25.
[204] Ideguchi N. Seed germination of Alaska iris, Iris setosa ssp[J].interior.Hort-Science, 1987,22(5): 898-899.
[205]刘德福,陈世璜.马蔺的繁殖特性及生态地理分布的研究[J].内蒙古农牧学院学,1998,19(1): 126.
[206]李菊艳,赵成义,孙栋元等.水分对胡杨幼苗光合及生长特性的影响[J].西北植物学报,2009,29(7): 1445-1451.
[207]陈健妙,掷青松,刘兆普等.麻疯树(Jatropha curcas L.)幼苗生长和光合作用对盐胁迫的响应[J].生态学报,2009,29(3):1356-1365.
[208]曹磊,袁玉欣,曹艳红等.田间和盆栽核桃与柿子苗木光合特性的初步研究[J].河北林果研究, 2005,20(1):26-29.
[209]秦嗣军,吕德国,刘国成等.盆栽樱桃植株的光合特性[J].园艺学报,2007,34(1):35-38.
[210]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.
[211]罗青红,李志军,伍维模等.胡杨、灰叶胡杨光合及叶绿素荧光特性的比较研究[J].西北植物学报,2006,26 (5):983-988.
[212]施征,史胜青,肖文发等.脱水胁迫对梭梭和胡杨苗叶绿素荧光特性的影响[J].林业科学研究, 2008,21(4):566-570.
[213]王蓟花,唐静,李端等.皂荚化学成分和生物活性的研究进展[J].中国野生植物资源,2008, 27(6):1-3.
[214]陈芳,陆斌,宁德鲁等.滇皂荚光合特性及其影响因子的研究[J].西北林学院学报,2005, 20(2):44-45.
[215]冯蕾,白志英,路丙社等.氯化钠胁迫对枳椇和皂荚生长、叶绿素荧光及活性氧代谢的影响[J].应用生态学报,2008,19(11):2503-250.
[216]罗俊,张木清,吕建林等.水分胁迫对不同甘蔗品种叶绿素a荧光动力学的影响[J].福建农业大学学报,2000 29(1):18-22.
[217]刘家尧,衣艳君,张其德.盐胁迫对不同抗旱性小麦叶片荧光诱导动力学影响[J].植物学通报, 1998,15(2):46-49.
[218]陈少良.杨树种间耐旱性差异的生理生化基础研究[D].北京林业大学博士论文,北京.1997, 50-51.
[219]Tezara W, Marín O, Rengifo E, et al. Photosynthesis and photoinhibition in two xerophytic shrubs during drought[J]. Photosynthetica, 2005, 43(1): 37-45.
[220]袁素芬,唐海萍.准噶尔荒漠3种短命植物气体交换特征的日变化[J].生态学报,2009,29(4): 1962-1970.
[221]宋丽丽,郭延平,徐凯等.温州蜜柑叶片光合作用光抑制的保护机理[J].应用生态学报,2003, 14(1): 47-50.
[222]Dodd I C, Critchley C, Woodall G S, et al. Photoinhibition in differently colored juvenile leaves of Syzygium species[J]. Journal Experimental of Botany, 1998, 49(325): 1437-1445.
[223]Demmig-Adams B, Adams III W W. Xanthophyll cycle and light stress in nature: uniform in response to excess direct sunlight among higher plant species[J]. Planta, 1996, 198(3): 460-470.
[224]毕春侠,张存旭,郭军战.杜仲多倍体诱导[J].河北林果研究,1996,14(2):148-150.
[225]Costich D E. Flow cytometer for rapid determination ofploidy level in vaccinium[J]. Theoreticaland ApplGenet , 1993, 86 (8): 120-126.
[226]范国强,杨志清,曹艳春.毛泡桐同源四倍体的诱导[J].植物生理学通讯,2007,43 (1): 109-111.
[227]高鹏,林威,康向阳.秋水仙碱诱导杜仲花粉染色体加倍的研究[J].北京林业大学学报,2004,26 (4):39-42.
[228]马爱红,范培格,孙建设.四倍体葡萄诱导技术的研究[J].中国农业科学,2005,38(8): 1645-1651.
[229]石荫坪,束怀瑞.利用苹果花粉粒形态进行倍性鉴定[J].园艺学报,1998,25 (2):133-138.
[230]王跃华.杜仲种子的多倍体诱导研究[J].亚太传统医药,2006. (8):73-76.
[231]杨晓伶,北岛宣,长谷川耕二郎.秋水仙素诱导文旦柚2n配子获得自交三倍体[J].园艺学报,2006,33 (5):1045-1047.
[232]张俊娥,刘继红,邓秀新.采用倍性分析仪鉴定柑橘愈伤组织的遗传变异[J].遗传学报,2003, 30(2):169-174.
[233]张蜀宁,万双粉,张伟.同源四倍体青花菜花粉母细胞的减数分裂[J].园艺学报,2007,34 (2): 387-390.
[234]ZhangX P,RhodesB B,Whitesides J F. Determination ofwatermelon ploidy levelusing flow cytometer[J]. CucurbitGenetCoop Rep.1994,17:102-105.
[235]张秀芳,石东里,张兰.观察植物气孔结构的简易方法[J].生物学通报,2002,37(6):42.
[236]张振超,张蜀宁,张伟.四倍体不结球白菜的诱导及染色体倍性鉴定[J].西北植物学报,2007, 27(1):28-32.
[237]张淑娟,刘与明,罗智凤.四季樱草的组织培养和快速繁殖.植物生理学通讯,2002,14(8):354.
[238]Matsubara ,S.Overcoming the self-incompatibility of Lilium longiflorum THUNB.by application of flower organextract or temperature treatment of pollen [J].Euphytica,1981,30:97-103.
[239]J.M.Vantuyl,M.Clara Marcucci and T.Visser.Pollen and pollination experiments :The effect of pollen treatment and application method onincompatibility and incongruity in lilium[J].Euphytica,1982,31:613-619.
[240]Shukla K S,Misra R L,Kaul M K,et al,Studies on pollen germination of certain fruits[J].Haryana J.Hortic.Sci,1978,7:162-164.
[2]Richards J.Primula[M].London:B. T. Batsford Ltd.1993.
[3]贾茵,张启翔.60Coγ-射线辐射对小报春种子萌发和M1性状的影响[J].种子,2009,28(11):1-3.
[4]李世峰,李伟琼.云南报春花种质资源分布特点及观赏类型[J].江西农业学报,2010,22(3): 85-87.
[5]赵艳昆,过永慧.昆明地区小报春的栽培[J].林业调查规划,2004(增刊):291-292.
[6]邓平平.六种报春花属植物的组织培养与多倍体诱导研究[D].北京林业大学,硕士论文,2007.
[7]梁树乐.我国西南地区部分报春花的引种与杂交育种研究[D].北京林业大学,博士论文,2006.
[8]胡伟娟,张启翔,潘会堂.报春叶片解剖结构与耐热性的关系[J].华中农业大学学报,2010,293(3):363-369.
[9]陈封怀,胡启明.中国报春花科植物系统分类研究.中国科学院院刊,1996(6):445~446.
[10]陈俊愉.国内外花卉科学研究与生产开发的现状与展望.中国园艺学会第八届年会上的报告,1997.
[11]李林光.苹果多倍体种质创新及鉴定评价研究[D].沈阳农业大学,博士论文,2008.
[12]刘小莉.报春花遗传变异的初步研究[D].云南大学,硕士论文,2004.
[13]刘飞虎,梁雪妮等.4种野生报春花光合特征的比较[J].园艺学报,2004,31(4):482-486.
[14]朱惠芬.五种观赏报春引种驯化的初步研究[D].中科院昆明植物研究所,硕士论文,2001.
[15]朱惠芬.九种报春花植物的核形态学研究[J].云南植物研究,2001,23(4):466-472.
[16] DafniA, HesseM,PaciniE. Polen and pollination[M]. New York:Springer-Verlag,2000,9:111–126.
[17]许玲.兰草再生与转化体系的建立及其离体诱变种质创新[D].浙江大学.博士论文,2009.
[18]贾文庆,刘会超,姚连芳.3个东方百合品种生物学特性观察及杂交亲和性研究[J].西北林学院学报,2007,22(6):18-20.
[19]刘海萍.药用植物茅苍术的组织培养[J].现代中药研究与实践,2005,19(5):10-12.
[20]Erica E.Benson,J.E.Danaher,In vitro micropropagation of Primula scotica:a rare Scottish plant[J],Biodiversity and Conservation 2000,9:711–726.
[21]娄和林.不同pH值对樱草原生质体分离的影响[J].植物学通报,1990,7(4):38-39.
[22]娄和林.四季樱草叶肉原生质体植株再生[J].辽宁大学学报,2005,9(5):19-21.
[23]程金水.园林植物遗传育种学[M].北京:中国林业出版社,2000.
[24]Coumans M,Gaspar Th.Mass propagation of Primula obconica[J].Acta Horticulturae.1979, 91:287-289.
[25]Margaret A,Webster P M.Acomparsion of early floral ontogeny in wild-type and floral homeotic mutant phenotypes of primula[J].Planta.2003,216:903-907.
[26]Kato J,Mii M.Differences in level of inter-specific hybrids obtained by reciprocal crosses between Primula seiboddii and Primula kisoana[J].Theor Appl Genet,2000,101:690-696.
[27]Karlsson M G.Primula culture and production[J].Hort Technology,2001,11(4):627-635.
[28]Yamamoto T.Mass propagation of Primula sieboldii E.Morr.through leat segment culture.Bulletianof the Faculty of Horticulture Minamikyushu University-Series [J].Natural Science.1999,29:9-14.
[29]Mizuhiro M.Prodction and characterization of interspecific somatic hybrids between Primula malacoides and P.obconica[J].Plant Sci.2001,161:489-496.
[30]Bajaj Y P S.Regeneration of plants from ultra-low frozen anthers of Primula obconica[J].Scientia Horti.1981,14(1):93-95.
[31]刘雪微.矮牵牛组织培养繁殖技术研究[J].现代园艺,2008,21(10):57-58.
[32]严春艳,马伟丽,于荣敏.长春花愈伤组织诱导及培养条件优化[J].中药材,2008,31(7):955-956.
[33]李娜.蝴蝶兰的组织培养技术研究[J].江西农业学报,2008,20(9):51-53.
[34]谭文澄,戴策刚.观赏植物组织培养技术[M].北京:中国林业出版社,1991.141.
[35]周家琪.花卉学[M].北京:中国林业出版社,1988.204.
[36]侯云屏,古志渊.报春花的组织培养和快速繁殖[J].植物生理学通讯,2001,37(3):234-235.
[37]李俊明.植物组织培养教程[M].北京:中国农业大学出版社,1992.
[38]颜昌敬.植物组织培养手册[M].上海:上海科学技术出版社.1980.
[39]韦三立.花卉组织培养[M].北京:中国林业出版社,2001.
[40]张树军,李元,韩冰.矮牵牛的组织培养与快繁[J].内蒙古农业科技,2001,7(4):16-18.
[41]李士安.植物组培在花卉上的应用[J].植物生理学通讯, 1988,20(1):69-71.
[42]刘青林.花卉组织培养[M].北京:中国农业出版社,2006.
[43]巩振辉.花卉脱毒与快繁新技术[M].杨凌:西北农林科技大学出版社,2005.
[44]王家福.花卉组织培养与快繁技术[M].北京:中国林业出版社,2006.
[45]唐道城,梁顺祥.观赏植物组织培养研究进展[J].青海大学学报(自然科学版),2006,24(4):5-9.
[46]陈黎.观赏植物的组织培养繁殖技术研究综述[J].黄山学院学报,2005,7(3):55-57.
[47]刘忠荣,陈屏昭.植物组织培养技术在观赏植物中的应用[J].昭通师范高等专科学校学报,2003,25(5):41-43.
[48]博术琳,程智慧,徐重益.植物组织培养快繁体系与产业化[J].现代化农业,2002,21(4):4-5.
[49]李景秀,孔繁才.高穗报春的引种栽培[J].云南农业科技,2000,19(3).17-19.
[50]朱惠芬.五种观赏报春引种驯化的初步研究[D].中国科学院昆明植物研究所硕士学位论文,2001.
[51]薛大伟,张长芹,黄媛.云南无量山报春花种质资源的调查[J].园艺学报.2003,30(4):476-478.
[52]袁亚夫,潘开文.瓦屋山地区野生报春花资源及其开发利用[J].山地学报,2000,18(2):263~266.
[53]Bruun H G..The cytology of the genus Primula (a preliminary report)[J].Svensk Bot Tidskr, 1930,24(3):468-475.
[54]Bruun H G,.Cytological studies in Primula [J].Symb Bot Upsala.1932,1:1-239.
[55]Nakata M,Wu Q A,Kurokawa S.Cytological studies on Chinese plants introduced from Yunnan Province.I. 1997.
[56]李晓林.低温和植物生长调节剂对桃花粉萌发的影响[J].西南农业大学学报,2005,27(4):526-529.
[57]张长芹.五种野生观赏报春花引种驯化初报[J].云南植物研究,2003,25(2):216~222.
[58]朱慧芬,张长芹,顾志建.九种报春花属植物的核形态学研究[J].云南植物研究,2001,23(4):466-472.
[59]薛大伟,张长芹.云南无量山四种报春花属植物的核型分析[J].云南植物研究,2003,25(1):78~82.
[60]Dalgard V.Additional chromosome numbers in vascular plants from the Disko Bugt area (West Greenland) [J] Willenouia, 1989,19:199-213.
[61]Loon J C V,Josina J M H O.Chromosome numbers of some angiosperms of the southern U.S.S.R[J].Act Bot Neerl, 1976,25(5):329-336.
[62]郝瑞娟,穆鼎,张檀等.百合品种间杂交的初步研究[J].西北林学院学报,2005,20(3):87-89.
[63]裴新澍.多倍体诱导与育种[M].上海科学技术出版社,1963,95-104.
[64]王鸿鹤,葛欣.秋水仙素诱导重瓣大岩桐多倍体研究.热带亚热带植物学报,1999,7(3):237-242.
[65]Sakaya S R,Joshi K K.Karymorphological studies in some Primula species of Nepal Himalaya [J].Cytologia, 1990,55:571-579.
[66]Sarkar A K.Cytotaxonomic studies of some members of Primulaceae from the Eastern Himalaya[J].Proc Indian Sci Congr Assoc, 1973,60:316.
[67]王长泉,张文胜.苹果叶片离体培养中秋水仙素加倍效应的研究[J].果树科学,2005,16(2):104-109.
[68]王敏琴,王晓红.葡萄多倍体诱导的初步研究[J].山东农业科学,2000,12(1):19-22.
[69]杨帆.薄壳山核桃及云南山核桃染色体核型分析[J].江西林业科技,2001,5(2):6-7.
[70]韩毅科,杜胜利,王明等.黄瓜染色体加倍研究[J].天津农业科技,2002,16(8):1-4.
[71]杜仙明,郑素秋.小白菜多倍体诱导试验[J].湖南农学院学报,1995,21(1):25-27.
[72]郭启高,张钟灵,周红等.秋水仙碱诱导生姜多倍体的研究[J].园艺学报,2000,12(5):400-402.
[73]李雪斌.兰州百合多倍体试验报告[J].甘肃农业科技,1995,16(6):14-15.
[74]Sarkar A K.Primulaceae-its evolution and assessment in status as judged through cytotaxonoy [J].Feddes Report, 1988,99:113-132.
[75]Shrama A,Sarkar A K.Chromosome numbers in some higher plants [J].The Research Bulletin, 1967,2:38-48.
[76]Tanaka R.Recent karyotype studies .In Ogawa K,et al.(eds):Plant Cytology[M].Tokyo:Asakura Shoten,1977,3:293-326.
[77]Wendelbo P..Studies in Primulaceae .I.A monographjof the genus Dionysia [J] .Arab Univ Bergen,Mat.Nat,1961,(3):1-83.
[78]Smith W W,Forrest G.The sections of the genus Primula[J].Journal of the Royal Horticultural Society,1928,54:4-114.
[79]Tanaka R.Types of resting nuclei in Orchidaceae [J].Bot Mag Tokyo,1971,84:118-112.
[80]Wendelbo P.Studies in Primulaceae . II.An account of Primula subgenus Sphondylia with a review of the subdivisions of the genus[J].Arab Univ Bergen,Mat.Nat,1961(11):1-49.
[81]彭华.滇中南无量山种子植物[M].昆明:云南科技出版社,1998.1-170.
[82]任唯勇.花卉和观赏植物种子发芽方法[J].种子,2001,23(4):466-472.
[83]乔玉刚,宋芸.蔬菜多倍体育种研究综述[J].长江蔬菜,2000,5(1):8-10.
[84]胡启明.种子植物科属地理[M].北京:科学出版社,1999,331-343.
[85]李长海.濒危岩报春的引种栽培[J].东北林业大学学报,1994, 22(2):54-59.
[86]曹仪植,宋占午.植物生理学[M].兰州大学出版社,1998.1-259.
[87] Richards J.Primula[M].Poland,OR:Timber Press,1993:3-4.
[88]高俊平,姜伟贤.中国花卉科技二十年.科学出版社,2000.
[89]李洁,张少艾.萱草属若干野生种园艺品种染色体核型的比较研究[J].上海农学院学报,1995,13(3):208-217.
[90]Mizuhiro M,Yamada K.Plant regeneration from cell suspension culture-derived protoplasts of Primula malaciodes and P.obconica[J].Plant Sci.2001,160:1221-1228.
[91]陈学森,邓秀新,章文才等.中国银杏资源染色体数目及核型研究初报[J].华中农业大学学报,1996,25(6):590-593.
[92]黄少甫,赵治芬.三种观赏植物的染色体研究[J].植物研究,1995,15(1):43-46.
[93]武全安.云南珍稀濒危植物的研究[J].植物引种驯化集刊,1990,23(4):173-179.
[94]胡叔良.草本植物引种驯化某些规律的探讨[J].植物研究,1987,21(2):301-306.
[95]林夏珍,赵建强.中国野生花卉种质资源调查综述[J].浙江林学院学报,2001,18(4):441-444.
[96]徐振华.ABT生根粉提高报春花种子活力的研究[J].河北林果研究,1999,14(3):234-237.
[97]范眸天.云南野生花卉[J].云南科技出版社,2001,12(3):12-15.
[98]李长海.濒危岩报春的引种栽培[J].东北林业大学学报,2005,12(3):22-25.
[99]郑维列.西藏色季拉山报春花种质资源及其生境类型[J].园艺学报,1992,19(3):261~266.
[100]吴之坤,张长芹.滇西北玉龙雪山报春花种质资源的调查[J].广西植物,2006,19(1):19-21.
[101]陈明林.安徽羽叶报春和毛茛叶报春的物种生物学研究[D].硕士论文,2002.05.
[102]赖力.峨嵋山报春花属植物引种研究[J].植物引种驯化集刊,1995年第10集.
[103]娄和林.四季樱草叶肉原生质体植株再生[J].辽宁大学学报,2005,23(l):58-59.
[104]郭清泉,郑思乡,杨瑞芳等.莲多倍体诱导及变异植物形态特征和细胞学观察[J].湖南农业大学学报,1997,23(1):26-29.
[105]栾岚,佟凤芹.不同培养基对四季樱草叶再生植株形成的影响[J].辽宁大学学报,1995,(3):122-126.
[106]XingHW,LaiL study on seed generination of primula[J].Plant Introduction and Acclimatization. 2001,11(4):627-635.
[107]胡伟娟,张启翔,潘会堂.报春花属植物耐热性生理指标研究初探[J].中国农学通报,2010,26(5):158-163.
[108]张云中,伍正容.多花樱草(Primula×polyantha Hort)自花授粉与异型花间授粉的研究[J].植物学集刊,1992,5:222-227.
[109]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究,1985,3(4):297-301.
[110]李懋学.植物染色体研究技术[M].哈尔滨:东北林业大学出版社,1991,3(4):131-145.
[111]幸宏伟,赖力.报春花属植物种子萌发的研究[J].植物引种驯化集刊,1997年第11集.
[112]Chen J F,Staub J E,Jiang J.A reevaluation of karyotype in Cucumber [J].Genet Res CropEvol,1998,45,301-305.
[113]Jaffe M J,Galston A W. The physiology of tendrils [J].Annu Rev Physiol,1986,19:417-434.
[114]Yadav R C ,Grumet R.Tendrils as an alternate tissue source for chromosome visualization [J].Journal of the American Society for Horticultural Science,1994,119(4):850-852.
[115]王月霞,王苏玲.微型水仙染色体C—带带型研究[J].园艺学报,1996,21(3):274-276.
[116]Brandham P.E.,Kirton P.R..The chromosomes of species,hybrids and cultivars of Narcissus L[J].Amaryllidaceae Kew Bulletin,1987,42(1):23-29.
[117]Gill.B.S,Friebe B.and Endo T.R.Standard karyotype and nomenclature system for description of chromosomes bands and structural aberrations in heat[J].Genome, 1991,l(34):830-839.
[118]杨继.植物多倍体基因组的形成与进化[J].植物分类学报,2001,39(4):357-371.
[119]郭启高,宋明,梁国鲁.植物多倍体诱导育种研究进展[J].生物学通报,2000,35(2):8-11.
[120]Hansen N.J.P,et al.In vitro chromosome doubling potential of colchicines,oryzalin,trifluralin,and APM in Brasica napus microspore culture[J].Euphytica,1996,(88):159-164.
[121]Chen CH,Goeden-kallemeyn YC.In vitro induction of tetraploid plants from colchicines-treated diploid daylily callus[J].Euphytics,1979,(28):705-709.
[122]陈发棣,蒋甲福,房伟民.秋水仙素诱导菊花脑多倍体的研究[J].上海农业学报,2002,(18):46-50.
[123]牛维和,徐玉冰,刘纪红.诱导君子兰多倍体的初步研究[J].园艺学报,1986,13(1):61-63.
[124]岳烨.诱导金鱼草多倍体的初步研究[J].园艺学报,1990,17(1):76-80.
[125]唐岱,熊济华,王仕玉.切花菊育种问有探讨[J].云南农业大学学报,2001,16(1):45-49.
[126]陈俊愉,王四清.花卉育种中的几个关键环节[J].园艺学报,1995,9(4):372-376.
[127]费水章.切花菊再生植株形态和细胞学变异的研究[J].园艺学报,1994,12(2):193-198.
[128]韩礼星,赵改荣,李玉红等.猕猴桃多倍体诱导研究[J].果树科学,1998,15(3),273-276.
[129]郑思乡,李立良.芦笋多倍体诱导及其离体培养的研究[J].湖南农业科学,1986,21(1):22-23.
[130]Goldblatt,P.Index to plant chromosome numbers 1975-1978[J].Missouri Bot.Garden,1981,142.
[131]Lee,Y.N.Chromosome numbers of flowering plants in korea.J[J].Korea Res.Inst.Ewha Women’s Unive.1967.11:455-478.
[132]Stebbins,G.L.Chromosomal evolutiong in higher plants[J] Edward Arnold Ltd,London. 1971:85-104.
[133]涂淑萍,傅波,喻苏琴.星冠水仙引种栽培及生物学特性研究[J].江西农业大学学报,2001, 21(1):96-98.
[134]姚志芬.哈尔滨市几种宿根花卉得应用[J].东北林业大学学报,2000,12(2):31-34.
[135]Iles-JK,Agnew-NH. Seasonal cold-acclimation patterns of Sedum spectabile x telephium L.’Autum Joy’and Sedum spectabile Boreau .’Brillant’[J].Hort Science.1995,30(6):1221-122.
[136]Dexfer ,S.T Toffingham and L.T[J].Craber.Plant physiol ,1930,5:215-213.
[137]Warry MOA ,et al .Reproductive response of cowpea to heat stress[J] .Crop Sci,1983 (23 ):1088.
[138]Rajashekar C ,Westwood MN .Deep supercooling and cold hardiness in genus pyrus[J].J Amer Soc Hort Sci,1982 ,107(6 ):968-972.
[139]Tahir M, Signh M. Assessment of screening techniques for heat tolerance in wheat[J]. Crop Sci,1993,33:740-744.
[140]Arora-R, Pitchay-DS,Beac-BC. Water-stress-induced heat tolerance in geranium leaf tissues: a possible linkage through stress proteins[J]. Phsiologia-Plantarum,1998, 103:1,24-34.
[141]Marcum-KB. Cell membrane thermostability and whole-plant heat tolerance of Kentucky bluegrass[J]. Crop Science,1998,38:1214-1218.
[142]Ranney TG, Peet MM. Heattolerance of five taxa birch:physiological responses to supraoptimal leaf temperatures. J. Amer. Hort. Sci. ,1994,119:243-248.
[143]Ranney TG,Blazich FA,Warren. Heat tolerance of selected species and populations of Rhodo-dendron[J]. JAmer Hort Sci. ,1995,120:423-428.
[144]Bauer-H, Nagele-M, Comploj-M, Galler-V,Mair-M, Unterpertinger-E. Photosynthesis after freezing stress in plants with various degreess of freezing tolerance[J]. Photosynthetica,1992, 27: 627-635 .
[145]Vierling A, Nguyen HT. Heat-shock gene expression in diploid wheat genotypes differing in ther-mal tolerance[J]. Crop Sci. ,1992,32:370-377.
[146]Youngner VB,Nudge FJ. Growth and carbohydrate storage of three Poa pratensis L. strains asinfluenced by temperature[J]. Crop Science,1968,8:455-457.
[147]Watschke TL,Schmidt RE. Some metabolic phenomena of Kentucky Bluegrass under high temperature[J].Crop Science,1972,12:87-90.
[148]吴应祥.中国兰花.北京:中国林业出版社,1991.
[149]田梅生,王伏雄,钱南芬等.四季兰种子离体萌发及器官建成的研究[J].植物学报,1985,27(5):455-459.
[150]王熊,吴敦肃.建兰类原球茎生长、发育过程中扫描电镜观察[J].植物生理学报,1991,17(2):192-196.
[151]张菊野,俞琳凤,连宏乾等.几种影响春兰原球茎生长与分化的因素[J].植物生理学通讯,1993,29(3):175-178.
[152]何立珍,周朴华,刘选明.黄花菜同源四倍体与二倍体形态及细胞遗传学分析[J].湖南农学院学报,1994,20(4):332-337.
[153]韩志慧,孟广云,赵素君.凤仙组织培养快繁技术的研究[J].天津农学院学报,2001,8(3):31-33.
[154]曾前.新几内亚凤仙的组织培养和快速繁[J]殖.中国林副特产,2002,(2):12-14.
[155]张立磊,杨和连,李靓.新几内亚凤仙的组培快繁研究[J].河南职业技术师范学院学报,2003,31(3):21-23.
[156]刘玉芹,王震星,李树和等.新几内亚凤仙组培快繁技术研究[J].天津农学院学报,2003,10(1):11-13.
[157]Cox.E.H.M.&Tayor,G.C. Primulas for Garden and Greenhouse.A.R.Mowbray CoLondon. 1957.
[158]张淑娟.四季樱草的组织培养和快速繁殖[J].植物生理学通讯,2002,38(4):354.
[159]徐振华.ABT生根粉提高报春花种子活力的研究[J].河北林果研究,1999,14(3):234-237.
[160]苏文化,张光飞.土壤温度与气温对紫花雪山报春光合作用和蒸腾作用的影响[J].西北植物学报,2002,22(4):824-830.
[161]刘飞虎,梁雪妮.4种野生报春花光合特征的比较[J].园艺学报,2004,31(4):482-486.
[162]李涵,龙春林,郑思乡.沉香虎头兰多倍体诱导及其鉴定[J].园艺学报,2005,32(5):853.
[163]张志胜,黎扬辉,姜蕾.红掌四倍体的离体诱导及其鉴定[J].园艺学报,2007.34 (3):729-734.
[164]郑思乡.东方百合多倍体诱导及种球繁育的研究[J].西南农业大学学报,2004,34 (3):46-49.
[165]郑思乡.离体培养条件下三色堇多倍体诱导研究[J].云南林业科技,2003,31(4):482-486.
[166]WaichiroK. Mass in vitro production of bulblets of Lilium japonicum Houtt[J]. J. Japan Soc. Hort. Sci, 1993, 61:611-618.
[167]张兴翠.花叶绿萝的多倍体诱导及快速繁殖[J].西南农业大学学报,2003,27(5):455-459.
[168]连雪斌.兰州百合多倍体诱导试验报告[J].甘肃农业科技,1995,12(6):14-18.
[169]康向阳,朱之悌.白杨2n花粉生命力测定方法及萌发特征的研究[J].云南植物研究, 1997,19(4):402-406.
[170]SternR A S Gazit.Lychee pollination by the oncybee[J].J.Amer.Soc.Hort.Sci,1996,120(1):152-157.
[171]Chen J F,Staub J E,Jiang J.A reevaluation of karyotype in Cucumber [J].Genet Res Crop Evol,1998,45:301-305.
[172]Jaffe M J,Galston A W. The physiology of tendrils [J].Annu Rev Physiol,1986,19:417-434.
[173]Yadav R C ,Grumet R.Tendrils as an alternate tissue source for chromosome visualization [J].Journal of the American Society for Horticultural Science,1994,119(4):850-852.
[174]Brandham P.E.,Kirton P.R. The chromosomes of species,hybrids and cultivars of Narcissus L[J].Amaryllidaceae Kew Bulletin,1987,42(1):23-37.
[175]Gill.B.S,Friebe B.and Endo T.R..Standard karyotype and nomenclature system for description of chromosomes bands and structural aberrations in wheat[J].Genome,1991,34:830-839.
[176]Hansen N.J.P,et al.In vitro chromosome doubling potential of colchicines,oryzalin,trifluralin,and APM in Brasica napus microspore culture[J].Euphytica,1996,(88):159-164.
[177]Chen CH,Goeden-kallemeyn YC.In vitro induction of tetraploid plants from colchicines-treated diploid daylily callus[J].Euphytics,1979,(28):705-709.
[178]Goldblatt,P.Index to plant chromosome numbers 1975-1978.Missouri Bot.Garden,1981,142.
[179]Lee,Y.N.Chromosome numbers of flowering plants in korea.J.Korea Res.Inst.Ewha Women’s Unive,1967,11:455-478.
[180]Stebbins,G.L.Chromosomal evolutiong in higher plants. Edward Arnold Ltd,London. 1971:85-104.
[181]Ahloowalia BS,MaluszynskiM. Inducedmutations-A new paradigm in plantbreeding[J].Euphytica, 2001,18(2): 67-173.
[182]郭安熙,杨保安,范家霖等.金光四射等六个菊花新品种的辐射选育[J].核农学通报,1991,12(2):73-75.
[183]牛传堂,何道一,李雅志.辐射诱变梅花突变体的研究[J].核农学报,1995,9(3):144-148.
[184]陈秀兰,包建忠,刘春贵等.观赏荷花辐射诱变育种初报[J].核农学报,2004,18(3):201-203.
[185]吴光升,强继业,陈立.60Coγ-射线对美女樱、菠萝菊根长、芽长及出芽率的影响[ J].安徽农业科学, 2005, 33 (6):1 032-1 033.
[186]徐冠仁.植物诱变育种学[M].北京:中国农业出版社,1996:91.
[187]潘光辉,王文强,尹贤贵.电导法鉴定番茄耐热性与田间结果的比较[J]西南园艺,2001,21(2):26-27.
[188]张睿鹂.滇西北报春资源及滇北球花报春核心种质的研究[D].北京:北京林业大学,2008.
[189]陈立松,刘星辉.高温对桃和柚细胞膜透性和光合色素的影响[J].武汉植物学研究,1997,21(15):234-238.
[190]刘祖祺,张石城.植物抗性生理学[M].北京:中国农业出版社,1995:199-215.
[191]曾韶西,王以柔.低温下黄瓜幼苗子叶硫氢基(SH)含量变化与膜脂过氧化[J].植物学报,1991,33(1):50-54.
[192]王荣富.植物抗寒指标的种类及其应用[J].河北农业大学学报,1987,12(3):49-55.
[193]汤章城.植物抗逆性生理生化研究的某些进展[J].植物生理学通讯,1991,27(2):146-148.
[194]叶陈亮,柯玉陈,陈伟.大白菜耐热性的生理研究.叶片水分和蛋白质代谢与耐热性[J].福建农业大学学报,1996,9(25):490-493.
[195]孟焕文,张彦峰,程智慧.黄瓜幼苗对热胁迫的生理反应及耐热鉴定指标筛选[J].西北农业学报,2000,9(1):47-49.
[196]张伟艳,王玲,卓丽环等.东北3种野生鸢尾种子生物学比较研究[J].种子, 2006,25(12): 34-37.
[197]刘强,卓丽环,陈杰等.野鸢尾种子开花结实生物学特性[J].东北林业大学学报, 2005,33(1): 47-48.
[198]曲寿河,刘玉章,孙伟等.野鸢尾的栽培及利用[J].特种经济动植物, 2008(8):19-20.
[199]刘强.野鸢尾生物学特性的研究[D].东北林业大学硕士论文,2005,1-45.
[200]彭幼芬.种子生理学[M].湖南:中南工业大学出版社,1994:262-266.
[201]王桂芹.不同生态环境马蔺植物体解剖结构比较[J].内蒙古民族大学学报:自然科学版,2002, 17(2):127-129.
[202]颜启传.种子学[M].北京:中国农业出版社,2001:91-101.
[203]沈云光,管开云,王仲朗等.四种国产鸢尾属植物种子萌发特性研究[J].种子,2005,24(12): 21-25.
[204] Ideguchi N. Seed germination of Alaska iris, Iris setosa ssp[J].interior.Hort-Science, 1987,22(5): 898-899.
[205]刘德福,陈世璜.马蔺的繁殖特性及生态地理分布的研究[J].内蒙古农牧学院学,1998,19(1): 126.
[206]李菊艳,赵成义,孙栋元等.水分对胡杨幼苗光合及生长特性的影响[J].西北植物学报,2009,29(7): 1445-1451.
[207]陈健妙,掷青松,刘兆普等.麻疯树(Jatropha curcas L.)幼苗生长和光合作用对盐胁迫的响应[J].生态学报,2009,29(3):1356-1365.
[208]曹磊,袁玉欣,曹艳红等.田间和盆栽核桃与柿子苗木光合特性的初步研究[J].河北林果研究, 2005,20(1):26-29.
[209]秦嗣军,吕德国,刘国成等.盆栽樱桃植株的光合特性[J].园艺学报,2007,34(1):35-38.
[210]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.
[211]罗青红,李志军,伍维模等.胡杨、灰叶胡杨光合及叶绿素荧光特性的比较研究[J].西北植物学报,2006,26 (5):983-988.
[212]施征,史胜青,肖文发等.脱水胁迫对梭梭和胡杨苗叶绿素荧光特性的影响[J].林业科学研究, 2008,21(4):566-570.
[213]王蓟花,唐静,李端等.皂荚化学成分和生物活性的研究进展[J].中国野生植物资源,2008, 27(6):1-3.
[214]陈芳,陆斌,宁德鲁等.滇皂荚光合特性及其影响因子的研究[J].西北林学院学报,2005, 20(2):44-45.
[215]冯蕾,白志英,路丙社等.氯化钠胁迫对枳椇和皂荚生长、叶绿素荧光及活性氧代谢的影响[J].应用生态学报,2008,19(11):2503-250.
[216]罗俊,张木清,吕建林等.水分胁迫对不同甘蔗品种叶绿素a荧光动力学的影响[J].福建农业大学学报,2000 29(1):18-22.
[217]刘家尧,衣艳君,张其德.盐胁迫对不同抗旱性小麦叶片荧光诱导动力学影响[J].植物学通报, 1998,15(2):46-49.
[218]陈少良.杨树种间耐旱性差异的生理生化基础研究[D].北京林业大学博士论文,北京.1997, 50-51.
[219]Tezara W, Marín O, Rengifo E, et al. Photosynthesis and photoinhibition in two xerophytic shrubs during drought[J]. Photosynthetica, 2005, 43(1): 37-45.
[220]袁素芬,唐海萍.准噶尔荒漠3种短命植物气体交换特征的日变化[J].生态学报,2009,29(4): 1962-1970.
[221]宋丽丽,郭延平,徐凯等.温州蜜柑叶片光合作用光抑制的保护机理[J].应用生态学报,2003, 14(1): 47-50.
[222]Dodd I C, Critchley C, Woodall G S, et al. Photoinhibition in differently colored juvenile leaves of Syzygium species[J]. Journal Experimental of Botany, 1998, 49(325): 1437-1445.
[223]Demmig-Adams B, Adams III W W. Xanthophyll cycle and light stress in nature: uniform in response to excess direct sunlight among higher plant species[J]. Planta, 1996, 198(3): 460-470.
[224]毕春侠,张存旭,郭军战.杜仲多倍体诱导[J].河北林果研究,1996,14(2):148-150.
[225]Costich D E. Flow cytometer for rapid determination ofploidy level in vaccinium[J]. Theoreticaland ApplGenet , 1993, 86 (8): 120-126.
[226]范国强,杨志清,曹艳春.毛泡桐同源四倍体的诱导[J].植物生理学通讯,2007,43 (1): 109-111.
[227]高鹏,林威,康向阳.秋水仙碱诱导杜仲花粉染色体加倍的研究[J].北京林业大学学报,2004,26 (4):39-42.
[228]马爱红,范培格,孙建设.四倍体葡萄诱导技术的研究[J].中国农业科学,2005,38(8): 1645-1651.
[229]石荫坪,束怀瑞.利用苹果花粉粒形态进行倍性鉴定[J].园艺学报,1998,25 (2):133-138.
[230]王跃华.杜仲种子的多倍体诱导研究[J].亚太传统医药,2006. (8):73-76.
[231]杨晓伶,北岛宣,长谷川耕二郎.秋水仙素诱导文旦柚2n配子获得自交三倍体[J].园艺学报,2006,33 (5):1045-1047.
[232]张俊娥,刘继红,邓秀新.采用倍性分析仪鉴定柑橘愈伤组织的遗传变异[J].遗传学报,2003, 30(2):169-174.
[233]张蜀宁,万双粉,张伟.同源四倍体青花菜花粉母细胞的减数分裂[J].园艺学报,2007,34 (2): 387-390.
[234]ZhangX P,RhodesB B,Whitesides J F. Determination ofwatermelon ploidy levelusing flow cytometer[J]. CucurbitGenetCoop Rep.1994,17:102-105.
[235]张秀芳,石东里,张兰.观察植物气孔结构的简易方法[J].生物学通报,2002,37(6):42.
[236]张振超,张蜀宁,张伟.四倍体不结球白菜的诱导及染色体倍性鉴定[J].西北植物学报,2007, 27(1):28-32.
[237]张淑娟,刘与明,罗智凤.四季樱草的组织培养和快速繁殖.植物生理学通讯,2002,14(8):354.
[238]Matsubara ,S.Overcoming the self-incompatibility of Lilium longiflorum THUNB.by application of flower organextract or temperature treatment of pollen [J].Euphytica,1981,30:97-103.
[239]J.M.Vantuyl,M.Clara Marcucci and T.Visser.Pollen and pollination experiments :The effect of pollen treatment and application method onincompatibility and incongruity in lilium[J].Euphytica,1982,31:613-619.
[240]Shukla K S,Misra R L,Kaul M K,et al,Studies on pollen germination of certain fruits[J].Haryana J.Hortic.Sci,1978,7:162-164.