重组马白细胞介素-18的鉴定及其定量抗原捕获ELISA检测方法的建立
|
摘要
白细胞介素-18(IL-18)是一种重要的细胞因子,它能作用于I型辅助性T细胞(Th1),并在IL-12的协同作用下能强烈诱导其产生IFN-γ。近来研究表明:IL-18具有广泛的生物学活性,在介导细胞免疫、抵抗微生物感染和抗肿瘤方面起着重要的作用。因此,IL-18在临床上可作为免疫增强剂、抗微生物或抗肿瘤制剂得以应用。到目前为止,国内外已有人、小鼠、猴、猪、牛和羊等种属的IL-18商品化制剂及其相应的单克隆抗体(McAb)或多克隆抗体(PcAb)以及夹心ELISA试剂盒的商品化供应。然而,有关马IL-18(EIL-18)的研究较少且相对滞后,迄今为止,还未见有可供商品化的EIL-18和抗EIL-18 McAb或PcAb及其夹心ELISA试剂盒等相关产品。
本研究采用RT-PCR从经ConA刺激的马外周血单核细胞(PBMCs)中扩增获得编码EIL-18前体蛋白(precursor EIL-18,pEIL-18)的DNA,然后克隆至载体pMD18-T中,鉴定正确的重组质粒命名为pMD-EIL-18。自pMD-EIL-18中分别扩增pEIL-18及其成熟蛋白(mature EIL-18,mEIL-18)基因,并将其亚克隆至原核表达载体pET-28a(+)和pET-26b(+)中,重组质粒鉴定正确后命名为pET-pEIL-18、pET-EIL-18和pET-mEIL-18。然后分别转化至大肠杆菌感受态BL21(DE3)中进行诱导表达,表达产物经SDS-PAGE分析,结果表明所得到重组蛋白分别约为25kD、20kD和18kD,经Western-blot鉴定结果表明这3种重组蛋白均能与兔抗人IL-18发生免疫学反应,将这3种表达的重组蛋白分别命名为rpEIL-18、rEIL-18和rmEIL-18。在此基础上,本研究建立了rpEIL-18、rEIL-18和rmEIL-18在非变性状态下通过镍柱亲和层析或偶联了抗EIL-18 McAb的CNBr-activated Sepharose 4B亲和层析纯化重组蛋白的方法,采用该方法纯化获得了高纯度的重组蛋白rpEIL-18、rEIL-18和rmEIL-18。为鉴定所获得的重组蛋白rpEIL-18、rEIL-18和rmEIL-18是否具有生物学活性,本研究还建立了一种检测马IFN-γmRNA拷贝数的实时荧光定量RT-PCR方法,并采用本方法评价了rpEIL-18、rEIL-18和rmEIL-18在重组人白细胞介素-12(rhIL-12)协同下在体外刺激马PBMCs所产生IFN-γ的状况。结果表明,rpEIL-18不具有相应的生物学活性,而rEIL-18和rmEIL-18具有类似天然EIL-18生物学活性的特性。
利用上述纯化后的rEIL-18抗原免疫新西兰白兔,制备了高效价的抗EIL-18 PcAb。同时,采用相同抗原免疫BALB/c小鼠,取脾细胞与SP2/0骨髓瘤细胞进行融合,以昆虫/杆状病毒表达系统获得的重组mEIL-18(rAcEIL-18)作为检测抗原进行间接ELISA检测,共筛选获得9株能稳定分泌抗体的单细胞克隆株。利用Western-blot和IFA实验对9株单克隆抗体(McAbs)进行鉴定,结果显示,所获得的9株细胞分泌的抗体均能与rEIL-18和rAcEIL-18发生特异性反应。将此9株McAbs分别与用原核系统分段表达的重组马白细胞介素-18成熟蛋白mEIL-181-78和mEIL-1879-157进行特异性ELISA检测,结果表明,其中5株能识别所表达的mEIL-181-78,而另外4株能与mEIL-1879-157发生反应,说明所获得的9株McAbs至少能针对rmEIL-18 2个或2个以上不同的抗原表位。采用单克隆抗体亚型鉴定试剂盒对该9株McAbs进行亚型鉴定,结果表明,除M1F11、M3A11和N7D9为IgM,其余的McAbs均为IgG1,而且所有McAbs的轻链均为κ链。将9株McAbs分别与rEIL-18和rmEIL-18进行中和活性测定,结果表明S1D7株对有生物活性的rEIL-18和rmEIL-18均具有中和效应。
将抗EIL-18的McAbs和PcAb进行粗提和亲和层析纯化,并对纯化后S6A3和B1G1株McAbs和PcAb进行生物素标记。通过对各株McAb或PcAb分别作为捕获抗体或检测抗体进行初步的配对筛选后,以亲和纯化的S6A3株McAb作为捕获抗体,生物素标记的B1G1株McAb作为检测抗体,经方阵试验优化抗原捕获ELISA反应体系,以不同浓度梯度的rEIL-18为捕获抗原进行ELISA检测绘制其OD值-抗原浓度标准曲线,确定检测灵敏度为15pg/mL。采用所建立的抗原捕获ELISA检测方法对健康和EIAV感染的马血清及猪、牛、羊血清进行检测,结果表明,猪血清、牛血清和羊血清在该检测方法中均无发生交叉反应性,健康马血清中EIL-18的含量在40-80pg/mL之间,而EIAV感染后的马血清中EIL-18含量略微升高,在70-150pg/mL之间。由于各种属的IL-18存在一定的交叉反应性,为了检测本研究所获得抗EIL-18 McAbs是否能够用于检测其他种属IL-18并确定其检测极限,参照配对筛选结果和上述建立好的EIL-18定量抗原捕获ELISA检测方法,以S1C6株McAb作为捕获抗体,生物素化的抗EIL-18 PcAb为检测抗体,商品化的重组猪IL-18(rPIL-18)为捕获抗原绘制OD值-抗原浓度标准曲线,确定检测灵敏度为30pg/mL,建立了基于抗EIL-18抗体为检测和捕获抗体的猪IL-18(PIL-18)定量抗原捕获ELISA检测方法;以S5F1株McAb作为捕获抗体,生物素化的抗EIL-18 PcAb为检测抗体,初步建立了以抗EIL-18抗体为检测和捕获抗体的牛IL-18(BIL-18)定量抗原捕获ELISA检测方法。
Interleukin-18 (IL-18) is an important cytokine, which can act on T helper 1-type T cells and strongly induce them to produce IFN-γin combination with IL-12. Pleiotropic effects of IL-18 have recently been reported. It plays an important role in the development of cell-mediated immune responses, defending the infection of microbial and resisting the tumor. Therefore, IL-18 may be used as an immune regulatory, anti-microbes and/or anti-tumor agent in clinic. So far, the products of human, mouse, monkey, porcine, bovine, goat IL-18 and the monoclonal or polyclonal antibody reagents against them have been developed, as well as the relative double-antibody ELISA kits. However, the function and relative studies of equine IL-18 (EIL-18) is still poorly understood. Even no any products of EIL-18, anti-EIL-18 monoclonal or polyclonal antibody or double-antibody ELISA kits come out yet.
In this study, the DNA of EIL-l8 was amplified by RT-PCR from the total RNA extracted from ConA stimulated equine PBMCs, and subsequently cloned into the vector pMD18-T, sequenced and named pMD-EIL-18. Then the genes encoding precursor and mature EIL-l8 were amplified from pMD-EIL-18 by PCR and subcloned into pET-28a(+) and pET-26b(+) respectively and recombinant plasmids (pET-pEIL-18, pET-EIL-18 and pET-mEIL-18) were obtained after sequencing confirmation. High levels of rpEIL-18, rEIL-18 and rmEIL-18 proteins were successfully expressed in E. coli strain BL21 (DE3) transformed with pET-pEIL-18, pET-EIL-18 and pET-mEIL-18. The SDS-PAGE and Western-blot analysis indicated that the fusion proteins were 25kD, 20kD and 18kD in molecular weight and had immunological reaction with rabbit against human IL-18 antibody. High-purity of recombinant proteins were recovered after purification with a Ni2+NTA column or CNBr-activated Sepharose 4B conjugated anti-EIL-18 McAb column. To identify whether or not rpEIL-18, rEIL-18 and rmEIL-18 proteins have biological activity, the method of real-time quantitative RT-PCR was established to detect the mRNA products of equine IFN-γfrom equine PBMCs stimulated with these recombinant proteins in vitro. The results showed that rEIL-18 and rmEIL-18 had a synergistic effect to induce the expression of IFN-γgene in equine PBMCs with the presence of recombinant human IL-12. All these results also indicated that the rEIL-18 and rmEIL-18 expressed in this study had biological activity commensurate with the native molecule. However, the rpEIL-18 had no such activity.
Rabbits were immunized subcutaneously with purified rEIL-18 proteins and high titers of PcAbs against EIL-18 were obtained subsequently. Meanwhile, BALB/c mice were immunized intraperitoneally with the same antigens for EIL-18 McAb preparation. After murine myeloma cells were fused with the splenocytes from the immunized mice, an indirect ELISA using the rAcEIL-18 protein derived from baculovirus expression system as antigen was carried to screen antibody-producing hybridomas. Consequently, nine McAbs against EIL-18 were got and Western-blot and IFA analysis showed all of them had positive reaction with rEIL-18 and rAcEIL-18 respectively. These 9 McAbs were used to react with mEIL-18 1-78aa and mEIL-18 79-157aa respectively in specific ELISA tests. As a result, five of them showed positive reaction to mEIL-18 1-78aa, the others had positive reaction to mEIL-18 79-157aa. McAb subtype identification kit was used to make sure which subtype these 9 McAb belonged to. The results showed three of them were IgM isotype and the rest ones belonged to IgG1 isotype, and the light chains of all McAbs wereκchain. Additional study displayed that the S1D7 McAb could effectively neutralize the bioactivity of rEIL-18 and rmEIL-18.
The McAbs and PcAb against EIL-18 were purified with extraction and immunoaffinity chromatography methods. And S6A3, B1G1 McAbs and PcAb were chosen to be biotinylated. After all of McAbs and PcAb were screened for the matched-pairs of double-antibody ELISA, a sandwich ELISA was developed to detect the EIL-18 by using purified S6A3 McAb as a capture antibody and the biotinylated B1G1 McAb as a detection antibody. And the limit of detection for the concentration of EIL-18 was 15pg/mL by this method.
Sera samples from health and EIAV-infected horse, pig, bovine and sheep were employed to measure the levels of IL-18 using the method established in this study. The results showed no cross-reaction occurred to pig, bovine and sheep sera samples in this testing method. What’s more, the IL-18 level from healthy horse serum was 40-80 pg/mL, while that was 70-150 pg/mL for EIAV-infected horse. Because of cross-reaction, porcine and bovine IL-18 quantitative sandwich ELISA was preliminarily established according to the EIL-18 quantitative sandwich ELISA. A sandwich ELISA for the detection of porcine IL-18 was developed using purified S1C6 McAb as a capture antibody and the biotinylated PcAb against EIL-18 as a detection antibody. The limit of detection for porcine IL-18 concentration was 30pg/mL in this sandwich ELISA. Additionally, the sandwich ELISA for the detection of bovine IL-18 was also preliminary established by using purified S5F1 McAb as a capture antibody and the biotinylated PcAb against EIL-18 as a detection antibody.
本研究采用RT-PCR从经ConA刺激的马外周血单核细胞(PBMCs)中扩增获得编码EIL-18前体蛋白(precursor EIL-18,pEIL-18)的DNA,然后克隆至载体pMD18-T中,鉴定正确的重组质粒命名为pMD-EIL-18。自pMD-EIL-18中分别扩增pEIL-18及其成熟蛋白(mature EIL-18,mEIL-18)基因,并将其亚克隆至原核表达载体pET-28a(+)和pET-26b(+)中,重组质粒鉴定正确后命名为pET-pEIL-18、pET-EIL-18和pET-mEIL-18。然后分别转化至大肠杆菌感受态BL21(DE3)中进行诱导表达,表达产物经SDS-PAGE分析,结果表明所得到重组蛋白分别约为25kD、20kD和18kD,经Western-blot鉴定结果表明这3种重组蛋白均能与兔抗人IL-18发生免疫学反应,将这3种表达的重组蛋白分别命名为rpEIL-18、rEIL-18和rmEIL-18。在此基础上,本研究建立了rpEIL-18、rEIL-18和rmEIL-18在非变性状态下通过镍柱亲和层析或偶联了抗EIL-18 McAb的CNBr-activated Sepharose 4B亲和层析纯化重组蛋白的方法,采用该方法纯化获得了高纯度的重组蛋白rpEIL-18、rEIL-18和rmEIL-18。为鉴定所获得的重组蛋白rpEIL-18、rEIL-18和rmEIL-18是否具有生物学活性,本研究还建立了一种检测马IFN-γmRNA拷贝数的实时荧光定量RT-PCR方法,并采用本方法评价了rpEIL-18、rEIL-18和rmEIL-18在重组人白细胞介素-12(rhIL-12)协同下在体外刺激马PBMCs所产生IFN-γ的状况。结果表明,rpEIL-18不具有相应的生物学活性,而rEIL-18和rmEIL-18具有类似天然EIL-18生物学活性的特性。
利用上述纯化后的rEIL-18抗原免疫新西兰白兔,制备了高效价的抗EIL-18 PcAb。同时,采用相同抗原免疫BALB/c小鼠,取脾细胞与SP2/0骨髓瘤细胞进行融合,以昆虫/杆状病毒表达系统获得的重组mEIL-18(rAcEIL-18)作为检测抗原进行间接ELISA检测,共筛选获得9株能稳定分泌抗体的单细胞克隆株。利用Western-blot和IFA实验对9株单克隆抗体(McAbs)进行鉴定,结果显示,所获得的9株细胞分泌的抗体均能与rEIL-18和rAcEIL-18发生特异性反应。将此9株McAbs分别与用原核系统分段表达的重组马白细胞介素-18成熟蛋白mEIL-181-78和mEIL-1879-157进行特异性ELISA检测,结果表明,其中5株能识别所表达的mEIL-181-78,而另外4株能与mEIL-1879-157发生反应,说明所获得的9株McAbs至少能针对rmEIL-18 2个或2个以上不同的抗原表位。采用单克隆抗体亚型鉴定试剂盒对该9株McAbs进行亚型鉴定,结果表明,除M1F11、M3A11和N7D9为IgM,其余的McAbs均为IgG1,而且所有McAbs的轻链均为κ链。将9株McAbs分别与rEIL-18和rmEIL-18进行中和活性测定,结果表明S1D7株对有生物活性的rEIL-18和rmEIL-18均具有中和效应。
将抗EIL-18的McAbs和PcAb进行粗提和亲和层析纯化,并对纯化后S6A3和B1G1株McAbs和PcAb进行生物素标记。通过对各株McAb或PcAb分别作为捕获抗体或检测抗体进行初步的配对筛选后,以亲和纯化的S6A3株McAb作为捕获抗体,生物素标记的B1G1株McAb作为检测抗体,经方阵试验优化抗原捕获ELISA反应体系,以不同浓度梯度的rEIL-18为捕获抗原进行ELISA检测绘制其OD值-抗原浓度标准曲线,确定检测灵敏度为15pg/mL。采用所建立的抗原捕获ELISA检测方法对健康和EIAV感染的马血清及猪、牛、羊血清进行检测,结果表明,猪血清、牛血清和羊血清在该检测方法中均无发生交叉反应性,健康马血清中EIL-18的含量在40-80pg/mL之间,而EIAV感染后的马血清中EIL-18含量略微升高,在70-150pg/mL之间。由于各种属的IL-18存在一定的交叉反应性,为了检测本研究所获得抗EIL-18 McAbs是否能够用于检测其他种属IL-18并确定其检测极限,参照配对筛选结果和上述建立好的EIL-18定量抗原捕获ELISA检测方法,以S1C6株McAb作为捕获抗体,生物素化的抗EIL-18 PcAb为检测抗体,商品化的重组猪IL-18(rPIL-18)为捕获抗原绘制OD值-抗原浓度标准曲线,确定检测灵敏度为30pg/mL,建立了基于抗EIL-18抗体为检测和捕获抗体的猪IL-18(PIL-18)定量抗原捕获ELISA检测方法;以S5F1株McAb作为捕获抗体,生物素化的抗EIL-18 PcAb为检测抗体,初步建立了以抗EIL-18抗体为检测和捕获抗体的牛IL-18(BIL-18)定量抗原捕获ELISA检测方法。
Interleukin-18 (IL-18) is an important cytokine, which can act on T helper 1-type T cells and strongly induce them to produce IFN-γin combination with IL-12. Pleiotropic effects of IL-18 have recently been reported. It plays an important role in the development of cell-mediated immune responses, defending the infection of microbial and resisting the tumor. Therefore, IL-18 may be used as an immune regulatory, anti-microbes and/or anti-tumor agent in clinic. So far, the products of human, mouse, monkey, porcine, bovine, goat IL-18 and the monoclonal or polyclonal antibody reagents against them have been developed, as well as the relative double-antibody ELISA kits. However, the function and relative studies of equine IL-18 (EIL-18) is still poorly understood. Even no any products of EIL-18, anti-EIL-18 monoclonal or polyclonal antibody or double-antibody ELISA kits come out yet.
In this study, the DNA of EIL-l8 was amplified by RT-PCR from the total RNA extracted from ConA stimulated equine PBMCs, and subsequently cloned into the vector pMD18-T, sequenced and named pMD-EIL-18. Then the genes encoding precursor and mature EIL-l8 were amplified from pMD-EIL-18 by PCR and subcloned into pET-28a(+) and pET-26b(+) respectively and recombinant plasmids (pET-pEIL-18, pET-EIL-18 and pET-mEIL-18) were obtained after sequencing confirmation. High levels of rpEIL-18, rEIL-18 and rmEIL-18 proteins were successfully expressed in E. coli strain BL21 (DE3) transformed with pET-pEIL-18, pET-EIL-18 and pET-mEIL-18. The SDS-PAGE and Western-blot analysis indicated that the fusion proteins were 25kD, 20kD and 18kD in molecular weight and had immunological reaction with rabbit against human IL-18 antibody. High-purity of recombinant proteins were recovered after purification with a Ni2+NTA column or CNBr-activated Sepharose 4B conjugated anti-EIL-18 McAb column. To identify whether or not rpEIL-18, rEIL-18 and rmEIL-18 proteins have biological activity, the method of real-time quantitative RT-PCR was established to detect the mRNA products of equine IFN-γfrom equine PBMCs stimulated with these recombinant proteins in vitro. The results showed that rEIL-18 and rmEIL-18 had a synergistic effect to induce the expression of IFN-γgene in equine PBMCs with the presence of recombinant human IL-12. All these results also indicated that the rEIL-18 and rmEIL-18 expressed in this study had biological activity commensurate with the native molecule. However, the rpEIL-18 had no such activity.
Rabbits were immunized subcutaneously with purified rEIL-18 proteins and high titers of PcAbs against EIL-18 were obtained subsequently. Meanwhile, BALB/c mice were immunized intraperitoneally with the same antigens for EIL-18 McAb preparation. After murine myeloma cells were fused with the splenocytes from the immunized mice, an indirect ELISA using the rAcEIL-18 protein derived from baculovirus expression system as antigen was carried to screen antibody-producing hybridomas. Consequently, nine McAbs against EIL-18 were got and Western-blot and IFA analysis showed all of them had positive reaction with rEIL-18 and rAcEIL-18 respectively. These 9 McAbs were used to react with mEIL-18 1-78aa and mEIL-18 79-157aa respectively in specific ELISA tests. As a result, five of them showed positive reaction to mEIL-18 1-78aa, the others had positive reaction to mEIL-18 79-157aa. McAb subtype identification kit was used to make sure which subtype these 9 McAb belonged to. The results showed three of them were IgM isotype and the rest ones belonged to IgG1 isotype, and the light chains of all McAbs wereκchain. Additional study displayed that the S1D7 McAb could effectively neutralize the bioactivity of rEIL-18 and rmEIL-18.
The McAbs and PcAb against EIL-18 were purified with extraction and immunoaffinity chromatography methods. And S6A3, B1G1 McAbs and PcAb were chosen to be biotinylated. After all of McAbs and PcAb were screened for the matched-pairs of double-antibody ELISA, a sandwich ELISA was developed to detect the EIL-18 by using purified S6A3 McAb as a capture antibody and the biotinylated B1G1 McAb as a detection antibody. And the limit of detection for the concentration of EIL-18 was 15pg/mL by this method.
Sera samples from health and EIAV-infected horse, pig, bovine and sheep were employed to measure the levels of IL-18 using the method established in this study. The results showed no cross-reaction occurred to pig, bovine and sheep sera samples in this testing method. What’s more, the IL-18 level from healthy horse serum was 40-80 pg/mL, while that was 70-150 pg/mL for EIAV-infected horse. Because of cross-reaction, porcine and bovine IL-18 quantitative sandwich ELISA was preliminarily established according to the EIL-18 quantitative sandwich ELISA. A sandwich ELISA for the detection of porcine IL-18 was developed using purified S1C6 McAb as a capture antibody and the biotinylated PcAb against EIL-18 as a detection antibody. The limit of detection for porcine IL-18 concentration was 30pg/mL in this sandwich ELISA. Additionally, the sandwich ELISA for the detection of bovine IL-18 was also preliminary established by using purified S5F1 McAb as a capture antibody and the biotinylated PcAb against EIL-18 as a detection antibody.
引文
1. 陈红英,崔保安,黄青云,李新生,王学斌.海兰鸡白细胞介素-18 全基因的克隆与序列分析.中国预防兽医学报 2006,28(1):48-51.
2. 陈红英,崔保安,夏平安,李新生,杨明凡,郑兰兰.麻鸭 IL-18 成熟蛋白基因的克隆、表达及其表达产物的生物学活性检测.农业生物技术学报 2007,15(4):584-588.
3. 陈红英,李新生,崔保安,夏平安,赵丽,方丽云.固始鸭白细胞介素-18 全基因克隆与分子进化分析. 中国预防兽医学报 2007,29(9): 676-679.
4. 宫强.牛结核病 DNA 疫苗的研究,[博士学位论文]. 北京:中国农业科学院研究生院, 2007.
5. 胡慧琼.四川山地乌骨鸡 IL-2、15、18、IFN-γ基因克隆测序及分子佐剂效应研究,[博士学位论文].四川:四川农业大学,2006.
6. 胡敬东,崔治中,赵宏坤.鸡 IL-18cDNA 的克隆及在大肠杆菌中的高效表达.畜牧兽医学报 2005,22(3 :264-268.
7. 金建平,汪美先,金伯泉.单克隆抗体亲和力的测定及其意义,细胞与分子免疫学杂志,Z1:107-110.
8. 李志中,秦立廷,王喜军,陈伟业,范蕾,李培锋,步志高.抗鸡γ-干扰素单克隆抗体的制备及定量抗原 ELISA 方法的建立. 中国预防兽医学报 2007,29(11):891-895.
9. 刘光亮.BF2/肽四聚体的构建及其在传染性支气管炎病毒特异性 T 细胞反应评价中的应用,[博士学位论文]. 北京:中国农业科学院研究生院,2007.
10. 潘蔚绮,刘胜旺,孔宪刚,李广兴,夏春.编码鸡 IL-18 成熟蛋白基因的分子克隆与序列测定.中国预防兽医学报 2003,25(2):114-117.
11. 沈关心,周汝麟.现代免疫学技术[M].第 2 版,湖北: 湖北科学技术出版社,2002,377-379.
12. 童铁钢,白宇,徐树兰,刘光亮,肖一红,王群,张维军,吴东来.马白细胞介素-18 单克隆抗体的制备及其特性的初步鉴定.农业生物技术学报 2007,15(5):762-766.
13. 童铁钢,刘光亮,白宇,肖一红,王群,李少英,孟庆文,吴东来.马白细胞介素 18 成熟蛋白(mEIL-18)基因在大肠埃希氏菌中的高效表达与纯化.畜牧兽医学报 2007,38(3):307-312.
14. 童铁钢,刘光亮,徐树兰,王群,肖一红,孟庆文,吴东来.马β2-微球蛋白基因的克隆及其在大肠埃希氏菌中的高效表达与纯化.中国兽医科技 2005,35(11):851-855.
15. 王福祥,杨宝山,马英骥,李用国.IL-12、IL-18 基因对 HIV-1 外膜蛋白基因疫苗诱导的免疫应答的影响.中华微生物学和免疫学杂志 2006,26(1):88-91.
16. 王群,刘光亮,童铁钢,肖一红,吴东来.鸡γ-干扰素实时荧光定量 RT-PCR 检测方法的建立.中国兽医科学 2007,37(7):631-635.
17. 吴欣,黄祖瑚,成军,邢益平,董菁.IL-12 和 IL-18 基因免疫对 HBcAg 核酸疫苗诱导小鼠(H-2d)特异性免疫应答的影响.中国免疫学杂志,2007,23(2):103-107.
18. 余斌,张强,闫丽萍,仇华吉,王云峰,陈焕春,童光志.抗猪 Gamma-干扰素单克隆抗体的制备和鉴定.中国预防兽医学报 2007,29(2):146-149.
19. 张立春,邓国华,刘振勇,田国彬,于康震,陈化兰. 具有 HI 活性的抗 H5 亚型流感病毒特异性单克隆抗体的研制.中国预防兽医学报 2005,27(5):329-332.
20. Akita, K., Ohtsuki, T., Nukada, Y., Tanimoto, T., Namba, M., Okura, T., Takakura-Yamamoto, R., Torigoe, K., Gu, Y., Su, M.S., Fujii, M., Satoh-Itoh, M., Yamamoto, K., Kohno, K., Ikeda, M., Kurimoto, M., 1997, Involvement of caspase-1 and caspase-3 in the production and processing of mature human interleukin 18 in monocytic THP.1 cells. J Biol Chem 272, 26595-26603.
21. Ancrile, B.B., O'Hayer, K.M., Counter, C.M., 2008, Oncogenic ras-induced expression of cytokines: a new target of anti-cancer therapeutics. Mol Interv 8, 22-27.
22. Argyle, D.J., McGillivery, C., Nicolson, L., Onions, D.E., 1999, Cloning, sequencing, and characterization of dog interleukin-18. Immunogenetics 49, 541-543.
23. Barbulescu, K., Becker, C., Schlaak, J.F., Schmitt, E., Meyer zum Buschenfelde, K.H., Neurath, M.F., 1998, IL-12 and IL-18 differentially regulate the transcriptional activity of the human IFN-gamma promoter in primary CD4+ T lymphocytes. J Immunol 160, 3642-3647.
24. Beadling, C., Slifka, M.K., 2005, Differential regulation of virus-specific T-cell effector functions following activation by peptide or innate cytokines. Blood 105, 1179-1186.
25. Beatty, J.D., Beatty, B.G., Vlahos, W.G., 1987, Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay. J Immunol Methods 100, 173-179.
26. Bohn, E., Sing, A., Zumbihl, R., Bielfeldt, C., Okamura, H., Kurimoto, M., Heesemann, J., Autenrieth, I.B., 1998, IL-18 (IFN-gamma-inducing factor) regulates early cytokine production in, and promotes resolution of, bacterial infection in mice. J Immunol 160, 299-307.
27. Bossi, G., Griffiths, G.M., 1999, Degranulation plays an essential part in regulating cell surface expression of Fas ligand in T cells and natural killer cells. Nat Med 5, 90-96.
28. Burstein, E., Fearon, E.R., 2008, Colitis and cancer: a tale of inflammatory cells and their cytokines. J Clin Invest 118, 464-467.
29. Cavalieri, H., Gamba, G., Courreges, M.C., Massouh, E.J., Benencia, F., 2005, Expression of IL-15, IL-18 and NOS-II in contralateral eyes of BALB/c mice during the development of HSV-induced keratitis. Immunol Lett 96, 295-298.
30. Chaudhury, P., Bera, B.C., 2005, Cloning and sequencing of Indian water buffalo interleukin-18 cDNA. Int J Immunogenet 32, 75-78.
31. Conti, B., Jahng, J.W., Tinti, C., Son, J.H., Joh, T.H., 1997, Induction of interferon-gamma inducing factor in the adrenal cortex. J Biol Chem 272, 2035-2037.
32. Conti, B., Park, L.C., Calingasan, N.Y., Kim, Y., Kim, H., Bae, Y., Gibson, G.E., Joh, T.H., 1999, Cultures of astrocytes and microglia express interleukin 18. Brain Res Mol Brain Res 67, 46-52.
33. Corbaz, A., ten Hove, T., Herren, S., Graber, P., Schwartsburd, B., Belzer, I., Harrison, J., Plitz, T., Kosco-Vilbois, M.H., Kim, S.H., Dinarello, C.A., Novick, D., van Deventer, S., Chvatchko, Y., 2002, IL-18-binding protein expression by endothelial cells and macrophages is up-regulated during active Crohn's disease. J Immunol 168, 3608-3616.
34. Coughlin, C.M., Salhany, K.E., Wysocka, M., Aruga, E., Kurzawa, H., Chang, A.E., Hunter, C.A., Fox, J.C., Trinchieri, G., Lee, W.M., 1998, Interleukin-12 and interleukin-18 synergistically induce murine tumor regression which involves inhibition of angiogenesis. J Clin Invest 101, 1441-1452.
35. Cui, F.D., Asada, H., Jin, M.L., Kishida, T., Shin-Ya, M., Nakaya, T., Kita, M., Ishii, M., Iwai, M., Okanoue, T., Imanishi, J., Mazda, O., 2005, Cytokine genetic adjuvant facilitates prophylactic intravascular DNA vaccine against acute and latent herpes simplex virus infection in mice. Gene Ther 12, 160-168.
36. Dai, S.M., Nishioka, K., Yudoh, K., 2004, Interleukin (IL) 18 stimulates osteoclast formation through synovial T cells in rheumatoid arthritis: comparison with IL1 beta and tumour necrosis factor alpha. Ann Rheum Dis 63, 1379-1386.
37. Dao, T., Mehal, W.Z., Crispe, I.N., 1998, IL-18 augments perforin-dependent cytotoxicity of liver NK-T cells. J Immunol 161, 2217-2222.
38. Delaleu, N., Bickel, M., 2004, Interleukin-1 beta and interleukin-18: regulation and activity inlocal inflammation. Periodontol 2000 35, 42-52.
39. Dinarello, C.A., 1998, Interleukin-1 beta, interleukin-18, and the interleukin-1 beta converting enzyme. Ann N Y Acad Sci 856, 1-11.
40. Dinarello, C.A., 2000, Targeting interleukin 18 with interleukin 18 binding protein. Ann Rheum Dis 59 Suppl 1, i17-20.
41. Dinarello, C.A., 2001, Novel targets for interleukin 18 binding protein. Ann Rheum Dis 60 Suppl 3, iii18-24.
42. Dinarello, C.A., Novick, D., Puren, A.J., Fantuzzi, G., Shapiro, L., Muhl, H., Yoon, D.Y., Reznikov, L.L., Kim, S.H., Rubinstein, M., 1998, Overview of interleukin-18: more than an interferon-gamma inducing factor. J Leukoc Biol 63, 658-664.
43. Dong, G., Liang, L., Fu, J., Zou, C., 2007, Serum interleukin-18 levels are raised in diabetic ketoacidosis in Chinese children with type 1 diabetes mellitus. Indian Pediatr 44, 732-736.
44. Duan, R.S., Zhang, X.M., Mix, E., Quezada, H.C., Adem, A., Zhu, J., 2007, IL-18 deficiency inhibits both Th1 and Th2 cytokine production but not the clinical symptoms in experimental autoimmune neuritis. J Neuroimmunol 183, 162-167.
45. Dudler, J., Simeoni, E., Fleury, S., Li, J., Pagnotta, M., Pascual, M., von Segesser, L.K., Vassalli, G., 2007, Gene transfer of interleukin-18-binding protein attenuates cardiac allograft rejection. Transpl Int 20, 460-466.
46. Eriksson, P., Andersson, C., Ekerfelt, C., Ernerudh, J., Skogh, T., 2004, Relationship between serum levels of IL-18 and IgG1 in patients with primary Sjogren's syndrome, rheumatoid arthritis and healthy controls. Clin Exp Immunol 137, 617-620.
47. Fantuzzi, G., Dinarello, C.A., 1999, Interleukin-18 and interleukin-1 beta: two cytokine substrates for ICE (caspase-1). J Clin Immunol 19, 1-11.
48. Fehniger, T.A., Shah, M.H., Turner, M.J., VanDeusen, J.B., Whitman, S.P., Cooper, M.A., Suzuki, K., Wechser, M., Goodsaid, F., Caligiuri, M.A., 1999, Differential cytokine and chemokine gene expression by human NK cells following activation with IL-18 or IL-15 in combination with IL-12: implications for the innate immune response. J Immunol 162, 4511-4520.
49. Ferrantini, M., Capone, I., Belardelli, F., 2008, Dendritic cells and cytokines in immune rejection of cancer. Cytokine Growth Factor Rev 19, 93-107.
50. Fournout, S., Dozois, C.M., Yerle, M., Pinton, P., Fairbrother, J.M., Oswald, E., Oswald, I.P.,2000, Cloning, chromosomal location, and tissue expression of the gene for pig interleukin-18. Immunogenetics 51, 358-365.
51. Fujioka, N., Akazawa, R., Ohashi, K., Fujii, M., Ikeda, M., Kurimoto, M., 1999, Interleukin-18 protects mice against acute herpes simplex virus type 1 infection. J Virol 73, 2401-2409.
52. Ghayur, T., Banerjee, S., Hugunin, M., Butler, D., Herzog, L., Carter, A., Quintal, L., Sekut, L., Talanian, R., Paskind, M., Wong, W., Kamen, R., Tracey, D., Allen, H., 1997, Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production. Nature 386, 619-623.
53. Gracie, J.A., Forsey, R.J., Chan, W.L., Gilmour, A., Leung, B.P., Greer, M.R., Kennedy, K., Carter, R., Wei, X.Q., Xu, D., Field, M., Foulis, A., Liew, F.Y., McInnes, I.B., 1999, A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest 104, 1393-1401.
54. Gu, Y., Kuida, K., Tsutsui, H., Ku, G., Hsiao, K., Fleming, M.A., Hayashi, N., Higashino, K., Okamura, H., Nakanishi, K., Kurimoto, M., Tanimoto, T., Flavell, R.A., Sato, V., Harding, M.W., Livingston, D.J., Su, M.S., 1997, Activation of interferon-gamma inducing factor mediated by interleukin-1beta converting enzyme. Science 275, 206-209.
55. Hanlon, L., Argyle, D., Bain, D., Nicolson, L., Dunham, S., Golder, M.C., McDonald, M., McGillivray, C., Jarrett, O., Neil, J.C., Onions, D.E., 2001, Feline leukemia virus DNA vaccine efficacy is enhanced by coadministration with interleukin-12 (IL-12) and IL-18 expression vectors. J Virol 75, 8424-8433.
56. Harishankar, M., Selvaraj, P., Rajeswari, D.N., Anand, S.P., Narayanan, P.R., 2007, Promoter polymorphism of IL-18 gene in pulmonary tuberculosis in South Indian population. Int J Immunogenet 34, 317-320.
57. Hashimoto, W., Osaki, T., Okamura, H., Robbins, P.D., Kurimoto, M., Nagata, S., Lotze, M.T., Tahara, H., 1999, Differential antitumor effects of administration of recombinant IL-18 or recombinant IL-12 are mediated primarily by Fas-Fas ligand- and perforin-induced tumor apoptosis, respectively. J Immunol 163, 583-589.
58. Henkart, P.A., 1996, ICE family proteases: mediators of all apoptotic cell death? Immunity 4, 195-201.
59. Heuer, J.G., Tucker-McClung, C., Hock, R.A., 1999, Neuroblastoma cells expressing mature IL-18, but not proIL-18, induce a strong and immediate antitumor immune response. J Immunother 22, 324-335.
60. Hibi, T., Kanai, T., Watanabe, M., Okazawa, S., Sato, T., 2000, [The role of IL-18 on thepathogenesis of Crohn's disease]. Nihon Rinsho Meneki Gakkai Kaishi 23, 607-610.
61. Hosamani, M., Mondal, B., Muneta, Y., Rasool, T.J., 2005, Molecular characterization and expression of caprine (Capra hircus) interleukin-18 cDNA. Int J Immunogenet 32, 293-297.
62. Hunter, C.A., Timans, J., Pisacane, P., Menon, S., Cai, G., Walker, W., Aste-Amezaga, M., Chizzonite, R., Bazan, J.F., Kastelein, R.A., 1997, Comparison of the effects of interleukin-1 alpha, interleukin-1 beta and interferon-gamma-inducing factor on the production of interferon-gamma by natural killer. Eur J Immunol 27, 2787-2792.
63. Hyodo, Y., Matsui, K., Hayashi, N., Tsutsui, H., Kashiwamura, S., Yamauchi, H., Hiroishi, K., Takeda, K., Tagawa, Y., Iwakura, Y., Kayagaki, N., Kurimoto, M., Okamura, H., Hada, T., Yagita, H., Akira, S., Nakanishi, K., Higashino, K., 1999, IL-18 up-regulates perforin-mediated NK activity without increasing perforin messenger RNA expression by binding to constitutively expressed IL-18 receptor. J Immunol 162, 1662-1668.
64. Ide, A., Kawasaki, E., Abiru, N., Sun, F., Kobayashi, M., Fukushima, T., Takahashi, R., Kuwahara, H., Kita, A., Oshima, K., Uotani, S., Yamasaki, H., Yamaguchi, Y., Eguchi, K., 2004, Association between IL-18 gene promoter polymorphisms and CTLA-4 gene 49A/G polymorphism in Japanese patients with type 1 diabetes. J Autoimmun 22, 73-78.
65. Jager, A., Sleijfer, S., van der Rijt, C.C., 2008, The pathogenesis of cancer related fatigue: could increased activity of pro-inflammatory cytokines be the common denominator? Eur J Cancer 44, 175-181.
66. Jander, S., Stoll, G., 1998, Differential induction of interleukin-12, interleukin-18, and interleukin-1beta converting enzyme mRNA in experimental autoimmune encephalomyelitis of the Lewis rat. J Neuroimmunol 91, 93-99.
67. Kanai, T., Uraushihara, K., Totsuka, T., Okazawa, A., Hibi, T., Oshima, S., Miyata, T., Nakamura, T., Watanabe, M., 2003, Macrophage-derived IL-18 targeting for the treatment of Crohn's disease. Curr Drug Targets Inflamm Allergy 2, 131-136.
68. Kanai, T., Watanabe, M., Okazawa, A., Sato, T., Yamazaki, M., Okamoto, S., Ishii, H., Totsuka, T., Iiyama, R., Okamoto, R., Ikeda, M., Kurimoto, M., Takeda, K., Akira, S., Hibi, T., 2001, Macrophage-derived IL-18-mediated intestinal inflammation in the murine model of Crohn's disease. Gastroenterology 121, 875-888.
69. Kawakami, K., Qureshi, M.H., Zhang, T., Okamura, H., Kurimoto, M., Saito, A., 1997, IL-18 protects mice against pulmonary and disseminated infection with Cryptococcus neoformans by inducing IFN-gamma production. J Immunol 159, 5528-5534.
70. Kawashima, M., Miossec, P., 2004, Decreased response to IL-12 and IL-18 of peripheral blood cells in rheumatoid arthritis. Arthritis Res Ther 6, R39-R45.
71. Kogut, M.H., Swaggerty, C., He, H., Pevzner, I., Kaiser, P., 2006, Toll-like receptor agonists stimulate differential functional activation and cytokine and chemokine gene expression in heterophils isolated from chickens with differential innate responses. Microbes Infect 8, 1866-1874.
72. Kohka, H., Yoshino, T., Iwagaki, H., Sakuma, I., Tanimoto, T., Matsuo, Y., Kurimoto, M., Orita, K., Akagi, T., Tanaka, N., 1998, Interleukin-18/interferon-gamma-inducing factor, a novel cytokine, up-regulates ICAM-1 (CD54) expression in KG-1 cells. J Leukoc Biol 64, 519-527.
73. Kohno, K., Kataoka, J., Ohtsuki, T., Suemoto, Y., Okamoto, I., Usui, M., Ikeda, M., Kurimoto, M., 1997, IFN-gamma-inducing factor (IGIF) is a costimulatory factor on the activation of Th1 but not Th2 cells and exerts its effect independently of IL-12. J Immunol 158, 1541-1550.
74. Kremer, L., Dupre, L., Wolowczuk, I., Locht, C., 1999, In vivo immunomodulation following intradermal injection with DNA encoding IL-18. J Immunol 163, 3226-3231.
75. Lachheb, J., Chelbi, H., Ammar, J., Hamzaoui, K., Hamzaoui, A., 2008, Promoter polymorphism of the IL-18 gene is associated with atopic asthma in Tunisian children. Int J Immunogenet 35, 63-68.
76. Lebel-Binay, S., Berger, A., Zinzindohoue, F., Cugnenc, P., Thiounn, N., Fridman, W.H., Pages, F., 2000, Interleukin-18: biological properties and clinical implications. Eur Cytokine Netw 11, 15-26.
77. Lian, H., Jin, N., Li, X., Mi, Z., Zhang, J., Sun, L., Li, X., Zheng, H., Li, P., 2007, Induction of an effective anti-tumor immune response and tumor regression by combined administration of IL-18 and Apoptin. Cancer Immunol Immunother 56, 181-192.
78. Lindegaard, B., Hansen, A.B., Gerstoft, J., Pedersen, B.K., 2004, High plasma level of interleukin-18 in HIV-infected subjects with lipodystrophy. J Acquir Immune Defic Syndr 36, 588-593.
79. Liu, G., Wang, Q., Tong, T., Xiao, Y., Bai, Y., Liu, S., Wu, D., 2008, Construction and functional test of a chicken MHC-I (BF2*15)/peptide tetramer. Vet Immunol Immunopathol 122, 1-7.
80. Liu, W.Q., Zhao, H.K., Gao, Y.D., Zhong, J.F., 2005, Cloning and expression of goat interleukin-18 gene. J Vet Med Sci 67, 219-221.
81. Ludwiczek, O., Kaser, A., Novick, D., Dinarello, C.A., Rubinstein, M., Tilg, H., 2005, Elevatedsystemic levels of free interleukin-18 (IL-18) in patients with Crohn's disease. Eur Cytokine Netw 16, 27-33.
82. Ma, M., Jin, N., Shen, G., Zhu, G., Liu, H.J., Zheng, M., Lu, H., Huo, X., Jin, M., Yin, G., Ma, H., Li, X., Ji, Y., Jin, K., 2008, Immune responses of swine inoculated with a recombinant fowlpox virus co-expressing P12A and 3C of FMDV and swine IL-18. Vet Immunol Immunopathol 121, 1-7.
83. Magram, J., Connaughton, S.E., Warrier, R.R., Carvajal, D.M., Wu, C.Y., Ferrante, J., Stewart, C., Sarmiento, U., Faherty, D.A., Gately, M.K., 1996a, IL-12-deficient mice are defective in IFN gamma production and type 1 cytokine responses. Immunity 4, 471-481.
84. Magram, J., Sfarra, J., Connaughton, S., Faherty, D., Warrier, R., Carvajal, D., Wu, C.Y., Stewart, C., Sarmiento, U., Gately, M.K., 1996b, IL-12-deficient mice are defective but not devoid of type 1 cytokine responses. Ann N Y Acad Sci 795, 60-70.
85. Malmgaard, L., 2004, Induction and regulation of IFNs during viral infections. J Interferon Cytokine Res 24, 439-454.
86. Martin, F., Santolaria, F., Batista, N., Milena, A., Gonzalez-Reimers, E., Brito, M.J., Oramas, J., 1999, Cytokine levels (IL-6 and IFN-gamma), acute phase response and nutritional status as prognostic factors in lung cancer. Cytokine 11, 80-86.
87. Mastroeni, P., Clare, S., Khan, S., Harrison, J.A., Hormaeche, C.E., Okamura, H., Kurimoto, M., Dougan, G., 1999, Interleukin 18 contributes to host resistance and gamma interferon production in mice infected with virulent Salmonella typhimurium. Infect Immun 67, 478-483.
88. McFadden, G., Murphy, P.M., 2000, Host-related immunomodulators encoded by poxviruses and herpesviruses. Curr Opin Microbiol 3, 371-378.
89. Meyer Zum Buschenfelde, C., Cramer, S., Trumpfheller, C., Fleischer, B., Frosch, S., 1997, Trypanosoma cruzi induces strong IL-12 and IL-18 gene expression in vivo: correlation with interferon-gamma (IFN-gamma) production. Clin Exp Immunol 110, 378-385.
90. Micallef, M.J., Ohtsuki, T., Kohno, K., Tanabe, F., Ushio, S., Namba, M., Tanimoto, T., Torigoe, K., Fujii, M., Ikeda, M., Fukuda, S., Kurimoto, M., 1996, Interferon-gamma-inducing factor enhances T helper 1 cytokine production by stimulated human T cells: synergism with interleukin-12 for interferon-gamma production. Eur J Immunol 26, 1647-1651.
91. Micallef, M.J., Yoshida, K., Kawai, S., Hanaya, T., Kohno, K., Arai, S., Tanimoto, T., Torigoe, K., Fujii, M., Ikeda, M., Kurimoto, M., 1997, In vivo antitumor effects of murine interferon-gamma-inducing factor/interleukin-18 in mice bearing syngeneic Meth A sarcomamalignant ascites. Cancer Immunol Immunother 43, 361-367.
92. Monteleone, G., Trapasso, F., Parrello, T., Biancone, L., Stella, A., Iuliano, R., Luzza, F., Fusco, A., Pallone, F., 1999, Bioactive IL-18 expression is up-regulated in Crohn's disease. J Immunol 163, 143-147.
93. Moreira, C., Tsuhako, M.H., de Franco, M.T., Modolell, M., Pereira, C.A., 2004, Arginine metabolism during macrophage autocrine activation and infection with mouse hepatitis virus 3. Immunobiology 209, 585-598.
94. Morita, M., Yoshida, R., Ikeda, K., Egashira, A., Oki, E., Sadanaga, N., Kakeji, Y., Ichiki, Y., Sugio, K., Yasumoto, K., Maehara, Y., 2008, Acute lung injury following an esophagectomy for esophageal cancer, with special reference to the clinical factors and cytokine levels of peripheral blood and pleural drainage fluid. Dis Esophagus 21, 30-36.
95. Munakata, T., Uzuki, M., Shimamura, T., Sawai, T., 2001, [Dynamics of interleukin (IL)-18 in serum, synovial fluid and synovial membrane in the patients with rheumatoid arthritis]. Ryumachi 41, 625-634.
96. Munder, M., Mallo, M., Eichmann, K., Modolell, M., 1998, Murine macrophages secrete interferon gamma upon combined stimulation with interleukin (IL)-12 and IL-18: A novel pathway of autocrine macrophage activation. J Exp Med 187, 2103-2108.
97. Muneta, Y., Goji, N., Tsuji, N.M., Mikami, O., Shimoji, Y., Nakajima, Y., Yokomizo, Y., Mori, Y., 2002, Expression of interleukin-18 by porcine airway and intestinal epithelium. J Interferon Cytokine Res 22, 883-889.
98. Muneta, Y., Inumaru, S., Shimoji, Y., Mori, Y., 2001, Efficient production of biologically active porcine interleukin-18 by coexpression with porcine caspase-1 using a baculovirus expression system. J Interferon Cytokine Res 21, 125-130.
99. Muneta, Y., Mikami, O., Shimoji, Y., Nakajima, Y., Yokomizo, Y., Mori, Y., 2000a, Detection of porcine interleukin-18 by sandwich ELISA and immunohistochemical staining using its monoclonal antibodies. J Interferon Cytokine Res 20, 331-336.
100. Muneta, Y., Mori, Y., Shimoji, Y., Yokomizo, Y., 2000b, Porcine interleukin 18: cloning, characterization of the cDNA and expression with the baculovirus system. Cytokine 12, 566-572.
101. Muneta, Y., Shimoji, Y., Yokomizo, Y., Mori, Y., 2000c, Production of monoclonal antibodies to porcine interleukin-18 and their use for immunoaffinity purification of recombinant porcine interleukin-18. J Immunol Methods 236, 99-104.
102. Muneta, Y., Yoshihara, K., Minagawa, Y., Nagata, R., Yasuyuki, M., Yamaguchi, T., Takehara, K., 2005, Bovine IL-18 ELISA: detection of IL-18 in sera of pregnant cow and newborn calf, and in colostrum. J Immunoassay Immunochem 26, 203-213.
103. Muneta, Y., Zhao, H.K., Inumaru, S., Mori, Y., 2003, Large-scale production of porcine mature interleukin-18 (IL-18) in silkworms using a hybrid baculovirus expression system. J Vet Med Sci 65, 219-223.
104. Nagata, T., Ishikawa, S., Shimokawa, E., Kamikawa, M., Kikuma, R., Muneta, Y., Yokomizo, Y., Nakamura, M., Takehara, K., 2002, High level expression and purification of bioactive bovine interleukin-18 using a baculovirus system. Vet Immunol Immunopathol 87, 65-72.
105. Nagaya, H., Muneta, Y., Enomoto, C., Matsumoto, S., Yokomizo, Y., Mori, Y., 2004, Method for purifying porcine mature interleukin-18 from silkworm haemolymph. Biotechnol Lett 26, 869-873.
106. Nicolson, L., Penha-Goncalves, M.N., Keanie, J.L., Logan, N.A., Argyle, D.J., Onions, D.E., 1999, Cloning and sequencing of horse interleukin-12 and interleukin-18 cDNAs. Immunogenetics 50, 94-97.
107. O'Donovan, L.H., McMonagle, E.L., Taylor, S., Argyle, D.J., Nicolson, L., 2004, Bioactivity and secretion of interleukin-18 (IL-18) generated by equine and feline IL-18 expression constructs. Vet Immunol Immunopathol 102, 421-428.
108. Oem, J.K., Song, H.J., Kang, S.W., Jeong, W.S., 2000, Cloning, sequencing, and expression of porcine interleukin-18 in Escherichia coli. Mol Cells 10, 343-347.
109. Okamoto, I., Kohno, K., Tanimoto, T., Ikegami, H., Kurimoto, M., 1999, Development of CD8+ effector T cells is differentially regulated by IL-18 and IL-12. J Immunol 162, 3202-3211.
110. Okamura, H., Nagata, K., Komatsu, T., Tanimoto, T., Nukata, Y., Tanabe, F., Akita, K., Torigoe, K., Okura, T., Fukuda, S., et al., 1995a, A novel costimulatory factor for gamma interferon induction found in the livers of mice causes endotoxic shock. Infect Immun 63, 3966-3972.
111. Okamura, H., Tsutsi, H., Komatsu, T., Yutsudo, M., Hakura, A., Tanimoto, T., Torigoe, K., Okura, T., Nukada, Y., Hattori, K., et al., 1995b, Cloning of a new cytokine that induces IFN-gamma production by T cells. Nature 378, 88-91.
112. Okamura, H., Tsutsui, H., Kashiwamura, S., Yoshimoto, T., Nakanishi, K., 1998, Interleukin-18: a novel cytokine that augments both innate and acquired immunity. Adv Immunol 70, 281-312.
113. Olee, T., Hashimoto, S., Quach, J., Lotz, M., 1999, IL-18 is produced by articular chondrocytesand induces proinflammatory and catabolic responses. J Immunol 162, 1096-1100.
114. Osaki, T., Peron, J.M., Cai, Q., Okamura, H., Robbins, P.D., Kurimoto, M., Lotze, M.T., Tahara, H., 1998, IFN-gamma-inducing factor/IL-18 administration mediates IFN-gamma- and IL-12-independent antitumor effects. J Immunol 160, 1742-1749.
115. Pages, F., Berger, A., Henglein, B., Piqueras, B., Danel, C., Zinzindohoue, F., Thiounn, N., Cugnenc, P.H., Fridman, W.H., 1999, Modulation of interleukin-18 expression in human colon carcinoma: consequences for tumor immune surveillance. Int J Cancer 84, 326-330.
116. Park, C., Lee, S., Cho, I.H., Lee, H.K., Kim, D., Choi, S.Y., Oh, S.B., Park, K., Kim, J.S., Lee, S.J., 2006, TLR3-mediated signal induces proinflammatory cytokine and chemokine gene expression in astrocytes: differential signaling mechanisms of TLR3-induced IP-10 and IL-8 gene expression. Glia 53, 248-256.
117. Peng, X., Kasran, A., Warmerdam, P.A., de Boer, M., Ceuppens, J.L., 1996, Accessory signaling by CD40 for T cell activation: induction of Th1 and Th2 cytokines and synergy with interleukin-12 for interferon-gamma production. Eur J Immunol 26, 1621-1627.
118. Petrovic-Rackov, L., 2006, [Evaluation of the degree of clinical rheumatoid arthritis activity based on the concentrations of cytokines TNF-alpha, IL-12, IL-15, and IL-18 in serum and synovial fluid]. Vojnosanit Pregl 63, 21-26.
119. Petrovic-Rackov, L., Pejnovic, N., 2006, Clinical significance of IL-18, IL-15, IL-12 and TNF-alpha measurement in rheumatoid arthritis. Clin Rheumatol 25, 448-452.
120. Pirhonen, J., 2001, Regulation of IL-18 expression in virus infection. Scand J Immunol 53, 533-539.
121. Pirhonen, J., Sareneva, T., Kurimoto, M., Julkunen, I., Matikainen, S., 1999, Virus infection activates IL-1 beta and IL-18 production in human macrophages by a caspase-1-dependent pathway. J Immunol 162, 7322-7329.
122. Pittoni, V., Bombardieri, M., Spinelli, F.R., Scrivo, R., Alessandri, C., Conti, F., Spadaro, A., Valesini, G., 2002, Anti-tumour necrosis factor (TNF) alpha treatment of rheumatoid arthritis (infliximab) selectively down regulates the production of interleukin (IL) 18 but not of IL12 and IL13. Ann Rheum Dis 61, 723-725.
123. Pizarro, T.T., Michie, M.H., Bentz, M., Woraratanadharm, J., Smith, M.F., Jr., Foley, E., Moskaluk, C.A., Bickston, S.J., Cominelli, F., 1999, IL-18, a novel immunoregulatory cytokine, is up-regulated in Crohn's disease: expression and localization in intestinal mucosal cells. J Immunol 162, 6829-6835.
124. Prinz, M., Hanisch, U.K., 1999, Murine microglial cells produce and respond to interleukin-18. J Neurochem 72, 2215-2218.
125. Puren, A.J., Fantuzzi, G., Gu, Y., Su, M.S., Dinarello, C.A., 1998a, Interleukin-18 (IFNgamma-inducing factor) induces IL-8 and IL-1beta via TNFalpha production from non-CD14+ human blood mononuclear cells. J Clin Invest 101, 711-721.
126. Puren, A.J., Razeghi, P., Fantuzzi, G., Dinarello, C.A., 1998b, Interleukin-18 enhances lipopolysaccharide-induced interferon-gamma production in human whole blood cultures. J Infect Dis 178, 1830-1834.
127. Qureshi, M.H., Zhang, T., Koguchi, Y., Nakashima, K., Okamura, H., Kurimoto, M., Kawakami, K., 1999, Combined effects of IL-12 and IL-18 on the clinical course and local cytokine production in murine pulmonary infection with Cryptococcus neoformans. Eur J Immunol 29, 643-649.
128. Rao, R.M., Telles, S., Nagendra, H.R., Nagarathna, R., Gopinath, K., Srinath, S., Chandrashekara, C., 2008, Effects of yoga on natural killer cell counts in early breast cancer patients undergoing conventional treatment Comment to: Recreational music-making modulates natural killer cell activity, cytokines, and mood states in corporate employees Masatada Wachi, Masahiro Koyama, Masanori Utsuyama, Barry B. Bittman, Masanobu Kitagawa, Katsuiku Hirokawa Med Sci Monit, 2007; 13(2): CR57-70. Med Sci Monit 14, LE3-4.
129. Raue, H.P., Brien, J.D., Hammarlund, E., Slifka, M.K., 2004, Activation of virus-specific CD8+ T cells by lipopolysaccharide-induced IL-12 and IL-18. J Immunol 173, 6873-6881.
130. Robinson, D., Shibuya, K., Mui, A., Zonin, F., Murphy, E., Sana, T., Hartley, S.B., Menon, S., Kastelein, R., Bazan, F., O'Garra, A., 1997, IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NFkappaB. Immunity 7, 571-581.
131. Rothe, H., Hibino, T., Itoh, Y., Kolb, H., Martin, S., 1997, Systemic production of interferon-gamma inducing factor (IGIF) versus local IFN-gamma expression involved in the development of Th1 insulitis in NOD mice. J Autoimmun 10, 251-256.
132. Sakao, Y., Takeda, K., Tsutsui, H., Kaisho, T., Nomura, F., Okamura, H., Nakanishi, K., Akira, S., 1999, IL-18-deficient mice are resistant to endotoxin-induced liver injury but highly susceptible to endotoxin shock. Int Immunol 11, 471-480.
133. Sareneva, T., Matikainen, S., Kurimoto, M., Julkunen, I., 1998, Influenza A virus-induced IFN-alpha/beta and IL-18 synergistically enhance IFN-gamma gene expression in human T cells.J Immunol 160, 6032-6038.
134. Sarvetnick, N., 1997, IFN-gamma, IGIF, and IDDM. J Clin Invest 99, 371-372.
135. Sato, M., Takemura, M., Shinohe, R., Koishi, H., Morita, T., Seishima, M., 2004, [Clinical significance of serum IL-18 determination in rheumatoid arthritis]. Rinsho Byori 52, 109-114.
136. Schneider, K., Puehler, F., Baeuerle, D., Elvers, S., Staeheli, P., Kaspers, B., Weining, K.C., 2000, cDNA cloning of biologically active chicken interleukin-18. J Interferon Cytokine Res 20, 879-883.
137. Seddi, R., Chaix, J.C., Puigserver, A., Guo, X.J., 2003, Expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Protein Expr Purif 27, 220-228.
138. Segat, L., Bevilacqua, D., Boniotto, M., Arraes, L.C., de Souza, P.R., de Lima Filho, J.L., Crovella, S., 2006, IL-18 gene promoter polymorphism is involved in HIV-1 infection in a Brazilian pediatric population. Immunogenetics.
139. Shen, G., Jin, N., Ma, M., Jin, K., Zheng, M., Zhuang, T., Lu, H., Zhu, G., Jin, H., Jin, M., Huo, X., Qin, X., Yin, R., Li, C., Li, H., Li, Y., Han, Z., Chen, Y., Jin, M., 2007, Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18. Vaccine 25, 4193-4202.
140. Shoda, L.K., Zarlenga, D.S., Hirano, A., Brown, W.C., 1999, Cloning of a cDNA encoding bovine interleukin-18 and analysis of IL-18 expression in macrophages and its IFN-gamma-inducing activity. J Interferon Cytokine Res 19, 1169-1177.
141. Spahr, L., Garcia, I., Bresson-Hadni, S., Rubbia-Brandt, L., Guler, R., Olleros, M., Chvatchko, Y., Hadengue, A., 2004, Circulating concentrations of interleukin-18, interleukin-18 binding protein, and gamma interferon in patients with alcoholic hepatitis. Liver Int 24, 582-587.
142. Splichalova, A., Trebichavsky, I., Muneta, Y., Mori, Y., Splichal, I., 2005, Effect of bacterial virulence on IL-18 expression in the amnion infected with Escherichia coli. Am J Reprod Immunol 53, 255-260.
143. Stoll, S., Jonuleit, H., Schmitt, E., Muller, G., Yamauchi, H., Kurimoto, M., Knop, J., Enk, A.H., 1998, Production of functional IL-18 by different subtypes of murine and human dendritic cells (DC): DC-derived IL-18 enhances IL-12-dependent Th1 development. Eur J Immunol 28, 3231-3239.
144. Stoll, S., Muller, G., Kurimoto, M., Saloga, J., Tanimoto, T., Yamauchi, H., Okamura, H., Knop, J., Enk, A.H., 1997, Production of IL-18 (IFN-gamma-inducing factor) messenger RNA andfunctional protein by murine keratinocytes. J Immunol 159, 298-302.
145. Stylianou, E., Bjerkeli, V., Yndestad, A., Heggelund, L., Waehre, T., Damas, J.K., Aukrust, P., Froland, S.S., 2003, Raised serum levels of interleukin-18 is associated with disease progression and may contribute to virological treatment failure in HIV-1-infected patients. Clin Exp Immunol 132, 462-466.
146. Sugawara, I., Yamada, H., Kaneko, H., Mizuno, S., Takeda, K., Akira, S., 1999, Role of interleukin-18 (IL-18) in mycobacterial infection in IL-18-gene-disrupted mice. Infect Immun 67, 2585-2589.
147. Takeda, K., Tsutsui, H., Yoshimoto, T., Adachi, O., Yoshida, N., Kishimoto, T., Okamura, H., Nakanishi, K., Akira, S., 1998, Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity 8, 383-390.
148. Takeuchi, M., Nishizaki, Y., Sano, O., Ohta, T., Ikeda, M., Kurimoto, M., 1997, Immunohistochemical and immuno-electron-microscopic detection of interferon-gamma-inducing factor ("interleukin-18") in mouse intestinal epithelial cells. Cell Tissue Res 289, 499-503.
149. Taniguchi, M., Nagaoka, K., Kunikata, T., Kayano, T., Yamauchi, H., Nakamura, S., Ikeda, M., Orita, K., Kurimoto, M., 1997, Characterization of anti-human interleukin-18 (IL-18)/interferon-gamma-inducing factor (IGIF) monoclonal antibodies and their application in the measurement of human IL-18 by ELISA. J Immunol Methods 206, 107-113.
150. Tartour, E., Gey, A., Sastre-Garau, X., Lombard Surin, I., Mosseri, V., Fridman, W.H., 1998, Prognostic value of intratumoral interferon gamma messenger RNA expression in invasive cervical carcinomas. J Natl Cancer Inst 90, 287-294.
151. Tomura, M., Maruo, S., Mu, J., Zhou, X.Y., Ahn, H.J., Hamaoka, T., Okamura, H., Nakanishi, K., Clark, S., Kurimoto, M., Fujiwara, H., 1998a, Differential capacities of CD4+, CD8+, and CD4-CD8- T cell subsets to express IL-18 receptor and produce IFN-gamma in response to IL-18. J Immunol 160, 3759-3765.
152. Tomura, M., Zhou, X.Y., Maruo, S., Ahn, H.J., Hamaoka, T., Okamura, H., Nakanishi, K., Tanimoto, T., Kurimoto, M., Fujiwara, H., 1998b, A critical role for IL-18 in the proliferation and activation of NK1.1+ CD3- cells. J Immunol 160, 4738-4746.
153. Tone, M., Thompson, S.A., Tone, Y., Fairchild, P.J., Waldmann, H., 1997, Regulation of IL-18 (IFN-gamma-inducing factor) gene expression. J Immunol 159, 6156-6163.
154. Trinchieri, G., 1998, Proinflammatory and immunoregulatory functions of interleukin-12. IntRev Immunol 16, 365-396.
155. Tsuji-Takayama, K., Matsumoto, S., Koide, K., Takeuchi, M., Ikeda, M., Ohta, T., Kurimoto, M., 1997, Interleukin-18 induces activation and association of p56(lck) and MAPK in a murine TH1 clone. Biochem Biophys Res Commun 237, 126-130.
156. Tsuji, H., Mukaida, N., Harada, A., Kaneko, S., Matsushita, E., Nakanuma, Y., Tsutsui, H., Okamura, H., Nakanishi, K., Tagawa, Y., Iwakura, Y., Kobayashi, K., Matsushima, K., 1999, Alleviation of lipopolysaccharide-induced acute liver injury in Propionibacterium acnes-primed IFN-gamma-deficient mice by a concomitant reduction of TNF-alpha, IL-12, and IL-18 production. J Immunol 162, 1049-1055.
157. Tsutsui, H., Matsui, K., Kawada, N., Hyodo, Y., Hayashi, N., Okamura, H., Higashino, K., Nakanishi, K., 1997, IL-18 accounts for both TNF-alpha- and Fas ligand-mediated hepatotoxic pathways in endotoxin-induced liver injury in mice. J Immunol 159, 3961-3967.
158. Tsutsui, H., Nakanishi, K., Matsui, K., Higashino, K., Okamura, H., Miyazawa, Y., Kaneda, K., 1996, IFN-gamma-inducing factor up-regulates Fas ligand-mediated cytotoxic activity of murine natural killer cell clones. J Immunol 157, 3967-3973.
159. Tuohy, V.K., Yu, M., Yin, L., Kawczak, J.A., Johnson, J.M., Mathisen, P.M., Weinstock-Guttman, B., Kinkel, R.P., 1998, The epitope spreading cascade during progression of experimental autoimmune encephalomyelitis and multiple sclerosis. Immunol Rev 164, 93-100.
160. Udagawa, N., Horwood, N.J., Elliott, J., Mackay, A., Owens, J., Okamura, H., Kurimoto, M., Chambers, T.J., Martin, T.J., Gillespie, M.T., 1997, Interleukin-18 (interferon-gamma-inducing factor) is produced by osteoblasts and acts via granulocyte/macrophage colony-stimulating factor and not via interferon-gamma to inhibit osteoclast formation. J Exp Med 185, 1005-1012.
161. Ushio, S., Namba, M., Okura, T., Hattori, K., Nukada, Y., Akita, K., Tanabe, F., Konishi, K., Micallef, M., Fujii, M., Torigoe, K., Tanimoto, T., Fukuda, S., Ikeda, M., Okamura, H., Kurimoto, M., 1996, Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein. J Immunol 156, 4274-4279.
162. Wheeler, R.D., Brough, D., Le Feuvre, R.A., Takeda, K., Iwakura, Y., Luheshi, G.N., Rothwell, N.J., 2003, Interleukin-18 induces expression and release of cytokines from murine glial cells: interactions with interleukin-1 beta. J Neurochem 85, 1412-1420.
163. Wiercinska-Drapalo, A., Jaroszewicz, J., Flisiak, R., Prokopowicz, D., 2004, Plasma interleukin-18 is associated with viral load and disease progression in HIV-1-infected patients. Microbes Infect 6, 1273-1277.
164. Wild, J.S., Sigounas, A., Sur, N., Siddiqui, M.S., Alam, R., Kurimoto, M., Sur, S., 2000, IFN-gamma-inducing factor (IL-18) increases allergic sensitization, serum IgE, Th2 cytokines, and airway eosinophilia in a mouse model of allergic asthma. J Immunol 164, 2701-2710.
165. Wildbaum, G., Youssef, S., Grabie, N., Karin, N., 1998, Neutralizing antibodies to IFN-gamma-inducing factor prevent experimental autoimmune encephalomyelitis. J Immunol 161, 6368-6374.
166. Wu, D., Murakami, K., Liu, N., Konishi, M., Muneta, Y., Inumaru, S., Kokuho, T., Sentsui, H., 2004, Expression of equine interleukin-18 by baculovirus expression system and its biologic activity. Microbiol Immunol 48, 471-476.
167. Xiang, Y., Moss, B., 2001, Determination of the functional epitopes of human interleukin-18-binding protein by site-directed mutagenesis. J Biol Chem 276, 17380-17386.
168. Xiang, Y., Moss, B., 2003, Molluscum contagiosum virus interleukin-18 (IL-18) binding protein is secreted as a full-length form that binds cell surface glycosaminoglycans through the C-terminal tail and a furin-cleaved form with only the IL-18 binding domain. J Virol 77, 2623-2630.
169. Xu, D., Chan, W.L., Leung, B.P., Hunter, D., Schulz, K., Carter, R.W., McInnes, I.B., Robinson, J.H., Liew, F.Y., 1998, Selective expression and functions of interleukin 18 receptor on T helper (Th) type 1 but not Th2 cells. J Exp Med 188, 1485-1492.
170. Xu, H.M., Zhang, G.Y., Ji, X.D., Cao, L., Shu, L., Hua, Z.C., 2005, Expression of soluble, biologically active recombinant human endostatin in Escherichia coli. Protein Expr Purif 41, 252-258.
171. Yamanaka, R., Tsuchiya, N., Yajima, N., Honma, J., Hasegawa, H., Tanaka, R., Ramsey, J., Blaese, R.M., Xanthopoulos, K.G., 2003, Induction of an antitumor immunological response by an intratumoral injection of dendritic cells pulsed with genetically engineered Semliki Forest virus to produce interleukin-18 combined with the systemic administration of interleukin-12. J Neurosurg 99, 746-753.
172. Yamanaka, R., Xanthopoulos, K.G., 2005, Induction of antigen-specific immune responses against malignant brain tumors by intramuscular injection of sindbis DNA encoding gp100 and IL-18. DNA Cell Biol 24, 317-324.
173. Yang, J., Murphy, T.L., Ouyang, W., Murphy, K.M., 1999, Induction of interferon-gamma production in Th1 CD4+ T cells: evidence for two distinct pathways for promoter activation. Eur J Immunol 29, 548-555.
174. Yoshimoto, T., Okamura, H., Tagawa, Y.I., Iwakura, Y., Nakanishi, K., 1997, Interleukin 18 together with interleukin 12 inhibits IgE production by induction of interferon-gamma production from activated B cells. Proc Natl Acad Sci U S A 94, 3948-3953.
175. Yoshimoto, T., Takeda, K., Tanaka, T., Ohkusu, K., Kashiwamura, S., Okamura, H., Akira, S., Nakanishi, K., 1998, IL-12 up-regulates IL-18 receptor expression on T cells, Th1 cells, and B cells: synergism with IL-18 for IFN-gamma production. J Immunol 161, 3400-3407.
176. Zecchina, G., Novick, D., Rubinstein, M., Barak, V., Dinarello, C., Nagler, A., 2001, Interleukin-18 binding protein in acute graft versus host disease and engraftment following allogeneic peripheral blood stem cell transplants. J Hematother Stem Cell Res 10, 769-776.
177. Zhai, Y., Kuick, R., Nan, B., Ota, I., Weiss, S.J., Trimble, C.L., Fearon, E.R., Cho, K.R., 2007, Gene expression analysis of preinvasive and invasive cervical squamous cell carcinomas identifies HOXC10 as a key mediator of invasion. Cancer Res 67, 10163-10172.
2. 陈红英,崔保安,夏平安,李新生,杨明凡,郑兰兰.麻鸭 IL-18 成熟蛋白基因的克隆、表达及其表达产物的生物学活性检测.农业生物技术学报 2007,15(4):584-588.
3. 陈红英,李新生,崔保安,夏平安,赵丽,方丽云.固始鸭白细胞介素-18 全基因克隆与分子进化分析. 中国预防兽医学报 2007,29(9): 676-679.
4. 宫强.牛结核病 DNA 疫苗的研究,[博士学位论文]. 北京:中国农业科学院研究生院, 2007.
5. 胡慧琼.四川山地乌骨鸡 IL-2、15、18、IFN-γ基因克隆测序及分子佐剂效应研究,[博士学位论文].四川:四川农业大学,2006.
6. 胡敬东,崔治中,赵宏坤.鸡 IL-18cDNA 的克隆及在大肠杆菌中的高效表达.畜牧兽医学报 2005,22(3 :264-268.
7. 金建平,汪美先,金伯泉.单克隆抗体亲和力的测定及其意义,细胞与分子免疫学杂志,Z1:107-110.
8. 李志中,秦立廷,王喜军,陈伟业,范蕾,李培锋,步志高.抗鸡γ-干扰素单克隆抗体的制备及定量抗原 ELISA 方法的建立. 中国预防兽医学报 2007,29(11):891-895.
9. 刘光亮.BF2/肽四聚体的构建及其在传染性支气管炎病毒特异性 T 细胞反应评价中的应用,[博士学位论文]. 北京:中国农业科学院研究生院,2007.
10. 潘蔚绮,刘胜旺,孔宪刚,李广兴,夏春.编码鸡 IL-18 成熟蛋白基因的分子克隆与序列测定.中国预防兽医学报 2003,25(2):114-117.
11. 沈关心,周汝麟.现代免疫学技术[M].第 2 版,湖北: 湖北科学技术出版社,2002,377-379.
12. 童铁钢,白宇,徐树兰,刘光亮,肖一红,王群,张维军,吴东来.马白细胞介素-18 单克隆抗体的制备及其特性的初步鉴定.农业生物技术学报 2007,15(5):762-766.
13. 童铁钢,刘光亮,白宇,肖一红,王群,李少英,孟庆文,吴东来.马白细胞介素 18 成熟蛋白(mEIL-18)基因在大肠埃希氏菌中的高效表达与纯化.畜牧兽医学报 2007,38(3):307-312.
14. 童铁钢,刘光亮,徐树兰,王群,肖一红,孟庆文,吴东来.马β2-微球蛋白基因的克隆及其在大肠埃希氏菌中的高效表达与纯化.中国兽医科技 2005,35(11):851-855.
15. 王福祥,杨宝山,马英骥,李用国.IL-12、IL-18 基因对 HIV-1 外膜蛋白基因疫苗诱导的免疫应答的影响.中华微生物学和免疫学杂志 2006,26(1):88-91.
16. 王群,刘光亮,童铁钢,肖一红,吴东来.鸡γ-干扰素实时荧光定量 RT-PCR 检测方法的建立.中国兽医科学 2007,37(7):631-635.
17. 吴欣,黄祖瑚,成军,邢益平,董菁.IL-12 和 IL-18 基因免疫对 HBcAg 核酸疫苗诱导小鼠(H-2d)特异性免疫应答的影响.中国免疫学杂志,2007,23(2):103-107.
18. 余斌,张强,闫丽萍,仇华吉,王云峰,陈焕春,童光志.抗猪 Gamma-干扰素单克隆抗体的制备和鉴定.中国预防兽医学报 2007,29(2):146-149.
19. 张立春,邓国华,刘振勇,田国彬,于康震,陈化兰. 具有 HI 活性的抗 H5 亚型流感病毒特异性单克隆抗体的研制.中国预防兽医学报 2005,27(5):329-332.
20. Akita, K., Ohtsuki, T., Nukada, Y., Tanimoto, T., Namba, M., Okura, T., Takakura-Yamamoto, R., Torigoe, K., Gu, Y., Su, M.S., Fujii, M., Satoh-Itoh, M., Yamamoto, K., Kohno, K., Ikeda, M., Kurimoto, M., 1997, Involvement of caspase-1 and caspase-3 in the production and processing of mature human interleukin 18 in monocytic THP.1 cells. J Biol Chem 272, 26595-26603.
21. Ancrile, B.B., O'Hayer, K.M., Counter, C.M., 2008, Oncogenic ras-induced expression of cytokines: a new target of anti-cancer therapeutics. Mol Interv 8, 22-27.
22. Argyle, D.J., McGillivery, C., Nicolson, L., Onions, D.E., 1999, Cloning, sequencing, and characterization of dog interleukin-18. Immunogenetics 49, 541-543.
23. Barbulescu, K., Becker, C., Schlaak, J.F., Schmitt, E., Meyer zum Buschenfelde, K.H., Neurath, M.F., 1998, IL-12 and IL-18 differentially regulate the transcriptional activity of the human IFN-gamma promoter in primary CD4+ T lymphocytes. J Immunol 160, 3642-3647.
24. Beadling, C., Slifka, M.K., 2005, Differential regulation of virus-specific T-cell effector functions following activation by peptide or innate cytokines. Blood 105, 1179-1186.
25. Beatty, J.D., Beatty, B.G., Vlahos, W.G., 1987, Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay. J Immunol Methods 100, 173-179.
26. Bohn, E., Sing, A., Zumbihl, R., Bielfeldt, C., Okamura, H., Kurimoto, M., Heesemann, J., Autenrieth, I.B., 1998, IL-18 (IFN-gamma-inducing factor) regulates early cytokine production in, and promotes resolution of, bacterial infection in mice. J Immunol 160, 299-307.
27. Bossi, G., Griffiths, G.M., 1999, Degranulation plays an essential part in regulating cell surface expression of Fas ligand in T cells and natural killer cells. Nat Med 5, 90-96.
28. Burstein, E., Fearon, E.R., 2008, Colitis and cancer: a tale of inflammatory cells and their cytokines. J Clin Invest 118, 464-467.
29. Cavalieri, H., Gamba, G., Courreges, M.C., Massouh, E.J., Benencia, F., 2005, Expression of IL-15, IL-18 and NOS-II in contralateral eyes of BALB/c mice during the development of HSV-induced keratitis. Immunol Lett 96, 295-298.
30. Chaudhury, P., Bera, B.C., 2005, Cloning and sequencing of Indian water buffalo interleukin-18 cDNA. Int J Immunogenet 32, 75-78.
31. Conti, B., Jahng, J.W., Tinti, C., Son, J.H., Joh, T.H., 1997, Induction of interferon-gamma inducing factor in the adrenal cortex. J Biol Chem 272, 2035-2037.
32. Conti, B., Park, L.C., Calingasan, N.Y., Kim, Y., Kim, H., Bae, Y., Gibson, G.E., Joh, T.H., 1999, Cultures of astrocytes and microglia express interleukin 18. Brain Res Mol Brain Res 67, 46-52.
33. Corbaz, A., ten Hove, T., Herren, S., Graber, P., Schwartsburd, B., Belzer, I., Harrison, J., Plitz, T., Kosco-Vilbois, M.H., Kim, S.H., Dinarello, C.A., Novick, D., van Deventer, S., Chvatchko, Y., 2002, IL-18-binding protein expression by endothelial cells and macrophages is up-regulated during active Crohn's disease. J Immunol 168, 3608-3616.
34. Coughlin, C.M., Salhany, K.E., Wysocka, M., Aruga, E., Kurzawa, H., Chang, A.E., Hunter, C.A., Fox, J.C., Trinchieri, G., Lee, W.M., 1998, Interleukin-12 and interleukin-18 synergistically induce murine tumor regression which involves inhibition of angiogenesis. J Clin Invest 101, 1441-1452.
35. Cui, F.D., Asada, H., Jin, M.L., Kishida, T., Shin-Ya, M., Nakaya, T., Kita, M., Ishii, M., Iwai, M., Okanoue, T., Imanishi, J., Mazda, O., 2005, Cytokine genetic adjuvant facilitates prophylactic intravascular DNA vaccine against acute and latent herpes simplex virus infection in mice. Gene Ther 12, 160-168.
36. Dai, S.M., Nishioka, K., Yudoh, K., 2004, Interleukin (IL) 18 stimulates osteoclast formation through synovial T cells in rheumatoid arthritis: comparison with IL1 beta and tumour necrosis factor alpha. Ann Rheum Dis 63, 1379-1386.
37. Dao, T., Mehal, W.Z., Crispe, I.N., 1998, IL-18 augments perforin-dependent cytotoxicity of liver NK-T cells. J Immunol 161, 2217-2222.
38. Delaleu, N., Bickel, M., 2004, Interleukin-1 beta and interleukin-18: regulation and activity inlocal inflammation. Periodontol 2000 35, 42-52.
39. Dinarello, C.A., 1998, Interleukin-1 beta, interleukin-18, and the interleukin-1 beta converting enzyme. Ann N Y Acad Sci 856, 1-11.
40. Dinarello, C.A., 2000, Targeting interleukin 18 with interleukin 18 binding protein. Ann Rheum Dis 59 Suppl 1, i17-20.
41. Dinarello, C.A., 2001, Novel targets for interleukin 18 binding protein. Ann Rheum Dis 60 Suppl 3, iii18-24.
42. Dinarello, C.A., Novick, D., Puren, A.J., Fantuzzi, G., Shapiro, L., Muhl, H., Yoon, D.Y., Reznikov, L.L., Kim, S.H., Rubinstein, M., 1998, Overview of interleukin-18: more than an interferon-gamma inducing factor. J Leukoc Biol 63, 658-664.
43. Dong, G., Liang, L., Fu, J., Zou, C., 2007, Serum interleukin-18 levels are raised in diabetic ketoacidosis in Chinese children with type 1 diabetes mellitus. Indian Pediatr 44, 732-736.
44. Duan, R.S., Zhang, X.M., Mix, E., Quezada, H.C., Adem, A., Zhu, J., 2007, IL-18 deficiency inhibits both Th1 and Th2 cytokine production but not the clinical symptoms in experimental autoimmune neuritis. J Neuroimmunol 183, 162-167.
45. Dudler, J., Simeoni, E., Fleury, S., Li, J., Pagnotta, M., Pascual, M., von Segesser, L.K., Vassalli, G., 2007, Gene transfer of interleukin-18-binding protein attenuates cardiac allograft rejection. Transpl Int 20, 460-466.
46. Eriksson, P., Andersson, C., Ekerfelt, C., Ernerudh, J., Skogh, T., 2004, Relationship between serum levels of IL-18 and IgG1 in patients with primary Sjogren's syndrome, rheumatoid arthritis and healthy controls. Clin Exp Immunol 137, 617-620.
47. Fantuzzi, G., Dinarello, C.A., 1999, Interleukin-18 and interleukin-1 beta: two cytokine substrates for ICE (caspase-1). J Clin Immunol 19, 1-11.
48. Fehniger, T.A., Shah, M.H., Turner, M.J., VanDeusen, J.B., Whitman, S.P., Cooper, M.A., Suzuki, K., Wechser, M., Goodsaid, F., Caligiuri, M.A., 1999, Differential cytokine and chemokine gene expression by human NK cells following activation with IL-18 or IL-15 in combination with IL-12: implications for the innate immune response. J Immunol 162, 4511-4520.
49. Ferrantini, M., Capone, I., Belardelli, F., 2008, Dendritic cells and cytokines in immune rejection of cancer. Cytokine Growth Factor Rev 19, 93-107.
50. Fournout, S., Dozois, C.M., Yerle, M., Pinton, P., Fairbrother, J.M., Oswald, E., Oswald, I.P.,2000, Cloning, chromosomal location, and tissue expression of the gene for pig interleukin-18. Immunogenetics 51, 358-365.
51. Fujioka, N., Akazawa, R., Ohashi, K., Fujii, M., Ikeda, M., Kurimoto, M., 1999, Interleukin-18 protects mice against acute herpes simplex virus type 1 infection. J Virol 73, 2401-2409.
52. Ghayur, T., Banerjee, S., Hugunin, M., Butler, D., Herzog, L., Carter, A., Quintal, L., Sekut, L., Talanian, R., Paskind, M., Wong, W., Kamen, R., Tracey, D., Allen, H., 1997, Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production. Nature 386, 619-623.
53. Gracie, J.A., Forsey, R.J., Chan, W.L., Gilmour, A., Leung, B.P., Greer, M.R., Kennedy, K., Carter, R., Wei, X.Q., Xu, D., Field, M., Foulis, A., Liew, F.Y., McInnes, I.B., 1999, A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest 104, 1393-1401.
54. Gu, Y., Kuida, K., Tsutsui, H., Ku, G., Hsiao, K., Fleming, M.A., Hayashi, N., Higashino, K., Okamura, H., Nakanishi, K., Kurimoto, M., Tanimoto, T., Flavell, R.A., Sato, V., Harding, M.W., Livingston, D.J., Su, M.S., 1997, Activation of interferon-gamma inducing factor mediated by interleukin-1beta converting enzyme. Science 275, 206-209.
55. Hanlon, L., Argyle, D., Bain, D., Nicolson, L., Dunham, S., Golder, M.C., McDonald, M., McGillivray, C., Jarrett, O., Neil, J.C., Onions, D.E., 2001, Feline leukemia virus DNA vaccine efficacy is enhanced by coadministration with interleukin-12 (IL-12) and IL-18 expression vectors. J Virol 75, 8424-8433.
56. Harishankar, M., Selvaraj, P., Rajeswari, D.N., Anand, S.P., Narayanan, P.R., 2007, Promoter polymorphism of IL-18 gene in pulmonary tuberculosis in South Indian population. Int J Immunogenet 34, 317-320.
57. Hashimoto, W., Osaki, T., Okamura, H., Robbins, P.D., Kurimoto, M., Nagata, S., Lotze, M.T., Tahara, H., 1999, Differential antitumor effects of administration of recombinant IL-18 or recombinant IL-12 are mediated primarily by Fas-Fas ligand- and perforin-induced tumor apoptosis, respectively. J Immunol 163, 583-589.
58. Henkart, P.A., 1996, ICE family proteases: mediators of all apoptotic cell death? Immunity 4, 195-201.
59. Heuer, J.G., Tucker-McClung, C., Hock, R.A., 1999, Neuroblastoma cells expressing mature IL-18, but not proIL-18, induce a strong and immediate antitumor immune response. J Immunother 22, 324-335.
60. Hibi, T., Kanai, T., Watanabe, M., Okazawa, S., Sato, T., 2000, [The role of IL-18 on thepathogenesis of Crohn's disease]. Nihon Rinsho Meneki Gakkai Kaishi 23, 607-610.
61. Hosamani, M., Mondal, B., Muneta, Y., Rasool, T.J., 2005, Molecular characterization and expression of caprine (Capra hircus) interleukin-18 cDNA. Int J Immunogenet 32, 293-297.
62. Hunter, C.A., Timans, J., Pisacane, P., Menon, S., Cai, G., Walker, W., Aste-Amezaga, M., Chizzonite, R., Bazan, J.F., Kastelein, R.A., 1997, Comparison of the effects of interleukin-1 alpha, interleukin-1 beta and interferon-gamma-inducing factor on the production of interferon-gamma by natural killer. Eur J Immunol 27, 2787-2792.
63. Hyodo, Y., Matsui, K., Hayashi, N., Tsutsui, H., Kashiwamura, S., Yamauchi, H., Hiroishi, K., Takeda, K., Tagawa, Y., Iwakura, Y., Kayagaki, N., Kurimoto, M., Okamura, H., Hada, T., Yagita, H., Akira, S., Nakanishi, K., Higashino, K., 1999, IL-18 up-regulates perforin-mediated NK activity without increasing perforin messenger RNA expression by binding to constitutively expressed IL-18 receptor. J Immunol 162, 1662-1668.
64. Ide, A., Kawasaki, E., Abiru, N., Sun, F., Kobayashi, M., Fukushima, T., Takahashi, R., Kuwahara, H., Kita, A., Oshima, K., Uotani, S., Yamasaki, H., Yamaguchi, Y., Eguchi, K., 2004, Association between IL-18 gene promoter polymorphisms and CTLA-4 gene 49A/G polymorphism in Japanese patients with type 1 diabetes. J Autoimmun 22, 73-78.
65. Jager, A., Sleijfer, S., van der Rijt, C.C., 2008, The pathogenesis of cancer related fatigue: could increased activity of pro-inflammatory cytokines be the common denominator? Eur J Cancer 44, 175-181.
66. Jander, S., Stoll, G., 1998, Differential induction of interleukin-12, interleukin-18, and interleukin-1beta converting enzyme mRNA in experimental autoimmune encephalomyelitis of the Lewis rat. J Neuroimmunol 91, 93-99.
67. Kanai, T., Uraushihara, K., Totsuka, T., Okazawa, A., Hibi, T., Oshima, S., Miyata, T., Nakamura, T., Watanabe, M., 2003, Macrophage-derived IL-18 targeting for the treatment of Crohn's disease. Curr Drug Targets Inflamm Allergy 2, 131-136.
68. Kanai, T., Watanabe, M., Okazawa, A., Sato, T., Yamazaki, M., Okamoto, S., Ishii, H., Totsuka, T., Iiyama, R., Okamoto, R., Ikeda, M., Kurimoto, M., Takeda, K., Akira, S., Hibi, T., 2001, Macrophage-derived IL-18-mediated intestinal inflammation in the murine model of Crohn's disease. Gastroenterology 121, 875-888.
69. Kawakami, K., Qureshi, M.H., Zhang, T., Okamura, H., Kurimoto, M., Saito, A., 1997, IL-18 protects mice against pulmonary and disseminated infection with Cryptococcus neoformans by inducing IFN-gamma production. J Immunol 159, 5528-5534.
70. Kawashima, M., Miossec, P., 2004, Decreased response to IL-12 and IL-18 of peripheral blood cells in rheumatoid arthritis. Arthritis Res Ther 6, R39-R45.
71. Kogut, M.H., Swaggerty, C., He, H., Pevzner, I., Kaiser, P., 2006, Toll-like receptor agonists stimulate differential functional activation and cytokine and chemokine gene expression in heterophils isolated from chickens with differential innate responses. Microbes Infect 8, 1866-1874.
72. Kohka, H., Yoshino, T., Iwagaki, H., Sakuma, I., Tanimoto, T., Matsuo, Y., Kurimoto, M., Orita, K., Akagi, T., Tanaka, N., 1998, Interleukin-18/interferon-gamma-inducing factor, a novel cytokine, up-regulates ICAM-1 (CD54) expression in KG-1 cells. J Leukoc Biol 64, 519-527.
73. Kohno, K., Kataoka, J., Ohtsuki, T., Suemoto, Y., Okamoto, I., Usui, M., Ikeda, M., Kurimoto, M., 1997, IFN-gamma-inducing factor (IGIF) is a costimulatory factor on the activation of Th1 but not Th2 cells and exerts its effect independently of IL-12. J Immunol 158, 1541-1550.
74. Kremer, L., Dupre, L., Wolowczuk, I., Locht, C., 1999, In vivo immunomodulation following intradermal injection with DNA encoding IL-18. J Immunol 163, 3226-3231.
75. Lachheb, J., Chelbi, H., Ammar, J., Hamzaoui, K., Hamzaoui, A., 2008, Promoter polymorphism of the IL-18 gene is associated with atopic asthma in Tunisian children. Int J Immunogenet 35, 63-68.
76. Lebel-Binay, S., Berger, A., Zinzindohoue, F., Cugnenc, P., Thiounn, N., Fridman, W.H., Pages, F., 2000, Interleukin-18: biological properties and clinical implications. Eur Cytokine Netw 11, 15-26.
77. Lian, H., Jin, N., Li, X., Mi, Z., Zhang, J., Sun, L., Li, X., Zheng, H., Li, P., 2007, Induction of an effective anti-tumor immune response and tumor regression by combined administration of IL-18 and Apoptin. Cancer Immunol Immunother 56, 181-192.
78. Lindegaard, B., Hansen, A.B., Gerstoft, J., Pedersen, B.K., 2004, High plasma level of interleukin-18 in HIV-infected subjects with lipodystrophy. J Acquir Immune Defic Syndr 36, 588-593.
79. Liu, G., Wang, Q., Tong, T., Xiao, Y., Bai, Y., Liu, S., Wu, D., 2008, Construction and functional test of a chicken MHC-I (BF2*15)/peptide tetramer. Vet Immunol Immunopathol 122, 1-7.
80. Liu, W.Q., Zhao, H.K., Gao, Y.D., Zhong, J.F., 2005, Cloning and expression of goat interleukin-18 gene. J Vet Med Sci 67, 219-221.
81. Ludwiczek, O., Kaser, A., Novick, D., Dinarello, C.A., Rubinstein, M., Tilg, H., 2005, Elevatedsystemic levels of free interleukin-18 (IL-18) in patients with Crohn's disease. Eur Cytokine Netw 16, 27-33.
82. Ma, M., Jin, N., Shen, G., Zhu, G., Liu, H.J., Zheng, M., Lu, H., Huo, X., Jin, M., Yin, G., Ma, H., Li, X., Ji, Y., Jin, K., 2008, Immune responses of swine inoculated with a recombinant fowlpox virus co-expressing P12A and 3C of FMDV and swine IL-18. Vet Immunol Immunopathol 121, 1-7.
83. Magram, J., Connaughton, S.E., Warrier, R.R., Carvajal, D.M., Wu, C.Y., Ferrante, J., Stewart, C., Sarmiento, U., Faherty, D.A., Gately, M.K., 1996a, IL-12-deficient mice are defective in IFN gamma production and type 1 cytokine responses. Immunity 4, 471-481.
84. Magram, J., Sfarra, J., Connaughton, S., Faherty, D., Warrier, R., Carvajal, D., Wu, C.Y., Stewart, C., Sarmiento, U., Gately, M.K., 1996b, IL-12-deficient mice are defective but not devoid of type 1 cytokine responses. Ann N Y Acad Sci 795, 60-70.
85. Malmgaard, L., 2004, Induction and regulation of IFNs during viral infections. J Interferon Cytokine Res 24, 439-454.
86. Martin, F., Santolaria, F., Batista, N., Milena, A., Gonzalez-Reimers, E., Brito, M.J., Oramas, J., 1999, Cytokine levels (IL-6 and IFN-gamma), acute phase response and nutritional status as prognostic factors in lung cancer. Cytokine 11, 80-86.
87. Mastroeni, P., Clare, S., Khan, S., Harrison, J.A., Hormaeche, C.E., Okamura, H., Kurimoto, M., Dougan, G., 1999, Interleukin 18 contributes to host resistance and gamma interferon production in mice infected with virulent Salmonella typhimurium. Infect Immun 67, 478-483.
88. McFadden, G., Murphy, P.M., 2000, Host-related immunomodulators encoded by poxviruses and herpesviruses. Curr Opin Microbiol 3, 371-378.
89. Meyer Zum Buschenfelde, C., Cramer, S., Trumpfheller, C., Fleischer, B., Frosch, S., 1997, Trypanosoma cruzi induces strong IL-12 and IL-18 gene expression in vivo: correlation with interferon-gamma (IFN-gamma) production. Clin Exp Immunol 110, 378-385.
90. Micallef, M.J., Ohtsuki, T., Kohno, K., Tanabe, F., Ushio, S., Namba, M., Tanimoto, T., Torigoe, K., Fujii, M., Ikeda, M., Fukuda, S., Kurimoto, M., 1996, Interferon-gamma-inducing factor enhances T helper 1 cytokine production by stimulated human T cells: synergism with interleukin-12 for interferon-gamma production. Eur J Immunol 26, 1647-1651.
91. Micallef, M.J., Yoshida, K., Kawai, S., Hanaya, T., Kohno, K., Arai, S., Tanimoto, T., Torigoe, K., Fujii, M., Ikeda, M., Kurimoto, M., 1997, In vivo antitumor effects of murine interferon-gamma-inducing factor/interleukin-18 in mice bearing syngeneic Meth A sarcomamalignant ascites. Cancer Immunol Immunother 43, 361-367.
92. Monteleone, G., Trapasso, F., Parrello, T., Biancone, L., Stella, A., Iuliano, R., Luzza, F., Fusco, A., Pallone, F., 1999, Bioactive IL-18 expression is up-regulated in Crohn's disease. J Immunol 163, 143-147.
93. Moreira, C., Tsuhako, M.H., de Franco, M.T., Modolell, M., Pereira, C.A., 2004, Arginine metabolism during macrophage autocrine activation and infection with mouse hepatitis virus 3. Immunobiology 209, 585-598.
94. Morita, M., Yoshida, R., Ikeda, K., Egashira, A., Oki, E., Sadanaga, N., Kakeji, Y., Ichiki, Y., Sugio, K., Yasumoto, K., Maehara, Y., 2008, Acute lung injury following an esophagectomy for esophageal cancer, with special reference to the clinical factors and cytokine levels of peripheral blood and pleural drainage fluid. Dis Esophagus 21, 30-36.
95. Munakata, T., Uzuki, M., Shimamura, T., Sawai, T., 2001, [Dynamics of interleukin (IL)-18 in serum, synovial fluid and synovial membrane in the patients with rheumatoid arthritis]. Ryumachi 41, 625-634.
96. Munder, M., Mallo, M., Eichmann, K., Modolell, M., 1998, Murine macrophages secrete interferon gamma upon combined stimulation with interleukin (IL)-12 and IL-18: A novel pathway of autocrine macrophage activation. J Exp Med 187, 2103-2108.
97. Muneta, Y., Goji, N., Tsuji, N.M., Mikami, O., Shimoji, Y., Nakajima, Y., Yokomizo, Y., Mori, Y., 2002, Expression of interleukin-18 by porcine airway and intestinal epithelium. J Interferon Cytokine Res 22, 883-889.
98. Muneta, Y., Inumaru, S., Shimoji, Y., Mori, Y., 2001, Efficient production of biologically active porcine interleukin-18 by coexpression with porcine caspase-1 using a baculovirus expression system. J Interferon Cytokine Res 21, 125-130.
99. Muneta, Y., Mikami, O., Shimoji, Y., Nakajima, Y., Yokomizo, Y., Mori, Y., 2000a, Detection of porcine interleukin-18 by sandwich ELISA and immunohistochemical staining using its monoclonal antibodies. J Interferon Cytokine Res 20, 331-336.
100. Muneta, Y., Mori, Y., Shimoji, Y., Yokomizo, Y., 2000b, Porcine interleukin 18: cloning, characterization of the cDNA and expression with the baculovirus system. Cytokine 12, 566-572.
101. Muneta, Y., Shimoji, Y., Yokomizo, Y., Mori, Y., 2000c, Production of monoclonal antibodies to porcine interleukin-18 and their use for immunoaffinity purification of recombinant porcine interleukin-18. J Immunol Methods 236, 99-104.
102. Muneta, Y., Yoshihara, K., Minagawa, Y., Nagata, R., Yasuyuki, M., Yamaguchi, T., Takehara, K., 2005, Bovine IL-18 ELISA: detection of IL-18 in sera of pregnant cow and newborn calf, and in colostrum. J Immunoassay Immunochem 26, 203-213.
103. Muneta, Y., Zhao, H.K., Inumaru, S., Mori, Y., 2003, Large-scale production of porcine mature interleukin-18 (IL-18) in silkworms using a hybrid baculovirus expression system. J Vet Med Sci 65, 219-223.
104. Nagata, T., Ishikawa, S., Shimokawa, E., Kamikawa, M., Kikuma, R., Muneta, Y., Yokomizo, Y., Nakamura, M., Takehara, K., 2002, High level expression and purification of bioactive bovine interleukin-18 using a baculovirus system. Vet Immunol Immunopathol 87, 65-72.
105. Nagaya, H., Muneta, Y., Enomoto, C., Matsumoto, S., Yokomizo, Y., Mori, Y., 2004, Method for purifying porcine mature interleukin-18 from silkworm haemolymph. Biotechnol Lett 26, 869-873.
106. Nicolson, L., Penha-Goncalves, M.N., Keanie, J.L., Logan, N.A., Argyle, D.J., Onions, D.E., 1999, Cloning and sequencing of horse interleukin-12 and interleukin-18 cDNAs. Immunogenetics 50, 94-97.
107. O'Donovan, L.H., McMonagle, E.L., Taylor, S., Argyle, D.J., Nicolson, L., 2004, Bioactivity and secretion of interleukin-18 (IL-18) generated by equine and feline IL-18 expression constructs. Vet Immunol Immunopathol 102, 421-428.
108. Oem, J.K., Song, H.J., Kang, S.W., Jeong, W.S., 2000, Cloning, sequencing, and expression of porcine interleukin-18 in Escherichia coli. Mol Cells 10, 343-347.
109. Okamoto, I., Kohno, K., Tanimoto, T., Ikegami, H., Kurimoto, M., 1999, Development of CD8+ effector T cells is differentially regulated by IL-18 and IL-12. J Immunol 162, 3202-3211.
110. Okamura, H., Nagata, K., Komatsu, T., Tanimoto, T., Nukata, Y., Tanabe, F., Akita, K., Torigoe, K., Okura, T., Fukuda, S., et al., 1995a, A novel costimulatory factor for gamma interferon induction found in the livers of mice causes endotoxic shock. Infect Immun 63, 3966-3972.
111. Okamura, H., Tsutsi, H., Komatsu, T., Yutsudo, M., Hakura, A., Tanimoto, T., Torigoe, K., Okura, T., Nukada, Y., Hattori, K., et al., 1995b, Cloning of a new cytokine that induces IFN-gamma production by T cells. Nature 378, 88-91.
112. Okamura, H., Tsutsui, H., Kashiwamura, S., Yoshimoto, T., Nakanishi, K., 1998, Interleukin-18: a novel cytokine that augments both innate and acquired immunity. Adv Immunol 70, 281-312.
113. Olee, T., Hashimoto, S., Quach, J., Lotz, M., 1999, IL-18 is produced by articular chondrocytesand induces proinflammatory and catabolic responses. J Immunol 162, 1096-1100.
114. Osaki, T., Peron, J.M., Cai, Q., Okamura, H., Robbins, P.D., Kurimoto, M., Lotze, M.T., Tahara, H., 1998, IFN-gamma-inducing factor/IL-18 administration mediates IFN-gamma- and IL-12-independent antitumor effects. J Immunol 160, 1742-1749.
115. Pages, F., Berger, A., Henglein, B., Piqueras, B., Danel, C., Zinzindohoue, F., Thiounn, N., Cugnenc, P.H., Fridman, W.H., 1999, Modulation of interleukin-18 expression in human colon carcinoma: consequences for tumor immune surveillance. Int J Cancer 84, 326-330.
116. Park, C., Lee, S., Cho, I.H., Lee, H.K., Kim, D., Choi, S.Y., Oh, S.B., Park, K., Kim, J.S., Lee, S.J., 2006, TLR3-mediated signal induces proinflammatory cytokine and chemokine gene expression in astrocytes: differential signaling mechanisms of TLR3-induced IP-10 and IL-8 gene expression. Glia 53, 248-256.
117. Peng, X., Kasran, A., Warmerdam, P.A., de Boer, M., Ceuppens, J.L., 1996, Accessory signaling by CD40 for T cell activation: induction of Th1 and Th2 cytokines and synergy with interleukin-12 for interferon-gamma production. Eur J Immunol 26, 1621-1627.
118. Petrovic-Rackov, L., 2006, [Evaluation of the degree of clinical rheumatoid arthritis activity based on the concentrations of cytokines TNF-alpha, IL-12, IL-15, and IL-18 in serum and synovial fluid]. Vojnosanit Pregl 63, 21-26.
119. Petrovic-Rackov, L., Pejnovic, N., 2006, Clinical significance of IL-18, IL-15, IL-12 and TNF-alpha measurement in rheumatoid arthritis. Clin Rheumatol 25, 448-452.
120. Pirhonen, J., 2001, Regulation of IL-18 expression in virus infection. Scand J Immunol 53, 533-539.
121. Pirhonen, J., Sareneva, T., Kurimoto, M., Julkunen, I., Matikainen, S., 1999, Virus infection activates IL-1 beta and IL-18 production in human macrophages by a caspase-1-dependent pathway. J Immunol 162, 7322-7329.
122. Pittoni, V., Bombardieri, M., Spinelli, F.R., Scrivo, R., Alessandri, C., Conti, F., Spadaro, A., Valesini, G., 2002, Anti-tumour necrosis factor (TNF) alpha treatment of rheumatoid arthritis (infliximab) selectively down regulates the production of interleukin (IL) 18 but not of IL12 and IL13. Ann Rheum Dis 61, 723-725.
123. Pizarro, T.T., Michie, M.H., Bentz, M., Woraratanadharm, J., Smith, M.F., Jr., Foley, E., Moskaluk, C.A., Bickston, S.J., Cominelli, F., 1999, IL-18, a novel immunoregulatory cytokine, is up-regulated in Crohn's disease: expression and localization in intestinal mucosal cells. J Immunol 162, 6829-6835.
124. Prinz, M., Hanisch, U.K., 1999, Murine microglial cells produce and respond to interleukin-18. J Neurochem 72, 2215-2218.
125. Puren, A.J., Fantuzzi, G., Gu, Y., Su, M.S., Dinarello, C.A., 1998a, Interleukin-18 (IFNgamma-inducing factor) induces IL-8 and IL-1beta via TNFalpha production from non-CD14+ human blood mononuclear cells. J Clin Invest 101, 711-721.
126. Puren, A.J., Razeghi, P., Fantuzzi, G., Dinarello, C.A., 1998b, Interleukin-18 enhances lipopolysaccharide-induced interferon-gamma production in human whole blood cultures. J Infect Dis 178, 1830-1834.
127. Qureshi, M.H., Zhang, T., Koguchi, Y., Nakashima, K., Okamura, H., Kurimoto, M., Kawakami, K., 1999, Combined effects of IL-12 and IL-18 on the clinical course and local cytokine production in murine pulmonary infection with Cryptococcus neoformans. Eur J Immunol 29, 643-649.
128. Rao, R.M., Telles, S., Nagendra, H.R., Nagarathna, R., Gopinath, K., Srinath, S., Chandrashekara, C., 2008, Effects of yoga on natural killer cell counts in early breast cancer patients undergoing conventional treatment Comment to: Recreational music-making modulates natural killer cell activity, cytokines, and mood states in corporate employees Masatada Wachi, Masahiro Koyama, Masanori Utsuyama, Barry B. Bittman, Masanobu Kitagawa, Katsuiku Hirokawa Med Sci Monit, 2007; 13(2): CR57-70. Med Sci Monit 14, LE3-4.
129. Raue, H.P., Brien, J.D., Hammarlund, E., Slifka, M.K., 2004, Activation of virus-specific CD8+ T cells by lipopolysaccharide-induced IL-12 and IL-18. J Immunol 173, 6873-6881.
130. Robinson, D., Shibuya, K., Mui, A., Zonin, F., Murphy, E., Sana, T., Hartley, S.B., Menon, S., Kastelein, R., Bazan, F., O'Garra, A., 1997, IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NFkappaB. Immunity 7, 571-581.
131. Rothe, H., Hibino, T., Itoh, Y., Kolb, H., Martin, S., 1997, Systemic production of interferon-gamma inducing factor (IGIF) versus local IFN-gamma expression involved in the development of Th1 insulitis in NOD mice. J Autoimmun 10, 251-256.
132. Sakao, Y., Takeda, K., Tsutsui, H., Kaisho, T., Nomura, F., Okamura, H., Nakanishi, K., Akira, S., 1999, IL-18-deficient mice are resistant to endotoxin-induced liver injury but highly susceptible to endotoxin shock. Int Immunol 11, 471-480.
133. Sareneva, T., Matikainen, S., Kurimoto, M., Julkunen, I., 1998, Influenza A virus-induced IFN-alpha/beta and IL-18 synergistically enhance IFN-gamma gene expression in human T cells.J Immunol 160, 6032-6038.
134. Sarvetnick, N., 1997, IFN-gamma, IGIF, and IDDM. J Clin Invest 99, 371-372.
135. Sato, M., Takemura, M., Shinohe, R., Koishi, H., Morita, T., Seishima, M., 2004, [Clinical significance of serum IL-18 determination in rheumatoid arthritis]. Rinsho Byori 52, 109-114.
136. Schneider, K., Puehler, F., Baeuerle, D., Elvers, S., Staeheli, P., Kaspers, B., Weining, K.C., 2000, cDNA cloning of biologically active chicken interleukin-18. J Interferon Cytokine Res 20, 879-883.
137. Seddi, R., Chaix, J.C., Puigserver, A., Guo, X.J., 2003, Expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Protein Expr Purif 27, 220-228.
138. Segat, L., Bevilacqua, D., Boniotto, M., Arraes, L.C., de Souza, P.R., de Lima Filho, J.L., Crovella, S., 2006, IL-18 gene promoter polymorphism is involved in HIV-1 infection in a Brazilian pediatric population. Immunogenetics.
139. Shen, G., Jin, N., Ma, M., Jin, K., Zheng, M., Zhuang, T., Lu, H., Zhu, G., Jin, H., Jin, M., Huo, X., Qin, X., Yin, R., Li, C., Li, H., Li, Y., Han, Z., Chen, Y., Jin, M., 2007, Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18. Vaccine 25, 4193-4202.
140. Shoda, L.K., Zarlenga, D.S., Hirano, A., Brown, W.C., 1999, Cloning of a cDNA encoding bovine interleukin-18 and analysis of IL-18 expression in macrophages and its IFN-gamma-inducing activity. J Interferon Cytokine Res 19, 1169-1177.
141. Spahr, L., Garcia, I., Bresson-Hadni, S., Rubbia-Brandt, L., Guler, R., Olleros, M., Chvatchko, Y., Hadengue, A., 2004, Circulating concentrations of interleukin-18, interleukin-18 binding protein, and gamma interferon in patients with alcoholic hepatitis. Liver Int 24, 582-587.
142. Splichalova, A., Trebichavsky, I., Muneta, Y., Mori, Y., Splichal, I., 2005, Effect of bacterial virulence on IL-18 expression in the amnion infected with Escherichia coli. Am J Reprod Immunol 53, 255-260.
143. Stoll, S., Jonuleit, H., Schmitt, E., Muller, G., Yamauchi, H., Kurimoto, M., Knop, J., Enk, A.H., 1998, Production of functional IL-18 by different subtypes of murine and human dendritic cells (DC): DC-derived IL-18 enhances IL-12-dependent Th1 development. Eur J Immunol 28, 3231-3239.
144. Stoll, S., Muller, G., Kurimoto, M., Saloga, J., Tanimoto, T., Yamauchi, H., Okamura, H., Knop, J., Enk, A.H., 1997, Production of IL-18 (IFN-gamma-inducing factor) messenger RNA andfunctional protein by murine keratinocytes. J Immunol 159, 298-302.
145. Stylianou, E., Bjerkeli, V., Yndestad, A., Heggelund, L., Waehre, T., Damas, J.K., Aukrust, P., Froland, S.S., 2003, Raised serum levels of interleukin-18 is associated with disease progression and may contribute to virological treatment failure in HIV-1-infected patients. Clin Exp Immunol 132, 462-466.
146. Sugawara, I., Yamada, H., Kaneko, H., Mizuno, S., Takeda, K., Akira, S., 1999, Role of interleukin-18 (IL-18) in mycobacterial infection in IL-18-gene-disrupted mice. Infect Immun 67, 2585-2589.
147. Takeda, K., Tsutsui, H., Yoshimoto, T., Adachi, O., Yoshida, N., Kishimoto, T., Okamura, H., Nakanishi, K., Akira, S., 1998, Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity 8, 383-390.
148. Takeuchi, M., Nishizaki, Y., Sano, O., Ohta, T., Ikeda, M., Kurimoto, M., 1997, Immunohistochemical and immuno-electron-microscopic detection of interferon-gamma-inducing factor ("interleukin-18") in mouse intestinal epithelial cells. Cell Tissue Res 289, 499-503.
149. Taniguchi, M., Nagaoka, K., Kunikata, T., Kayano, T., Yamauchi, H., Nakamura, S., Ikeda, M., Orita, K., Kurimoto, M., 1997, Characterization of anti-human interleukin-18 (IL-18)/interferon-gamma-inducing factor (IGIF) monoclonal antibodies and their application in the measurement of human IL-18 by ELISA. J Immunol Methods 206, 107-113.
150. Tartour, E., Gey, A., Sastre-Garau, X., Lombard Surin, I., Mosseri, V., Fridman, W.H., 1998, Prognostic value of intratumoral interferon gamma messenger RNA expression in invasive cervical carcinomas. J Natl Cancer Inst 90, 287-294.
151. Tomura, M., Maruo, S., Mu, J., Zhou, X.Y., Ahn, H.J., Hamaoka, T., Okamura, H., Nakanishi, K., Clark, S., Kurimoto, M., Fujiwara, H., 1998a, Differential capacities of CD4+, CD8+, and CD4-CD8- T cell subsets to express IL-18 receptor and produce IFN-gamma in response to IL-18. J Immunol 160, 3759-3765.
152. Tomura, M., Zhou, X.Y., Maruo, S., Ahn, H.J., Hamaoka, T., Okamura, H., Nakanishi, K., Tanimoto, T., Kurimoto, M., Fujiwara, H., 1998b, A critical role for IL-18 in the proliferation and activation of NK1.1+ CD3- cells. J Immunol 160, 4738-4746.
153. Tone, M., Thompson, S.A., Tone, Y., Fairchild, P.J., Waldmann, H., 1997, Regulation of IL-18 (IFN-gamma-inducing factor) gene expression. J Immunol 159, 6156-6163.
154. Trinchieri, G., 1998, Proinflammatory and immunoregulatory functions of interleukin-12. IntRev Immunol 16, 365-396.
155. Tsuji-Takayama, K., Matsumoto, S., Koide, K., Takeuchi, M., Ikeda, M., Ohta, T., Kurimoto, M., 1997, Interleukin-18 induces activation and association of p56(lck) and MAPK in a murine TH1 clone. Biochem Biophys Res Commun 237, 126-130.
156. Tsuji, H., Mukaida, N., Harada, A., Kaneko, S., Matsushita, E., Nakanuma, Y., Tsutsui, H., Okamura, H., Nakanishi, K., Tagawa, Y., Iwakura, Y., Kobayashi, K., Matsushima, K., 1999, Alleviation of lipopolysaccharide-induced acute liver injury in Propionibacterium acnes-primed IFN-gamma-deficient mice by a concomitant reduction of TNF-alpha, IL-12, and IL-18 production. J Immunol 162, 1049-1055.
157. Tsutsui, H., Matsui, K., Kawada, N., Hyodo, Y., Hayashi, N., Okamura, H., Higashino, K., Nakanishi, K., 1997, IL-18 accounts for both TNF-alpha- and Fas ligand-mediated hepatotoxic pathways in endotoxin-induced liver injury in mice. J Immunol 159, 3961-3967.
158. Tsutsui, H., Nakanishi, K., Matsui, K., Higashino, K., Okamura, H., Miyazawa, Y., Kaneda, K., 1996, IFN-gamma-inducing factor up-regulates Fas ligand-mediated cytotoxic activity of murine natural killer cell clones. J Immunol 157, 3967-3973.
159. Tuohy, V.K., Yu, M., Yin, L., Kawczak, J.A., Johnson, J.M., Mathisen, P.M., Weinstock-Guttman, B., Kinkel, R.P., 1998, The epitope spreading cascade during progression of experimental autoimmune encephalomyelitis and multiple sclerosis. Immunol Rev 164, 93-100.
160. Udagawa, N., Horwood, N.J., Elliott, J., Mackay, A., Owens, J., Okamura, H., Kurimoto, M., Chambers, T.J., Martin, T.J., Gillespie, M.T., 1997, Interleukin-18 (interferon-gamma-inducing factor) is produced by osteoblasts and acts via granulocyte/macrophage colony-stimulating factor and not via interferon-gamma to inhibit osteoclast formation. J Exp Med 185, 1005-1012.
161. Ushio, S., Namba, M., Okura, T., Hattori, K., Nukada, Y., Akita, K., Tanabe, F., Konishi, K., Micallef, M., Fujii, M., Torigoe, K., Tanimoto, T., Fukuda, S., Ikeda, M., Okamura, H., Kurimoto, M., 1996, Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein. J Immunol 156, 4274-4279.
162. Wheeler, R.D., Brough, D., Le Feuvre, R.A., Takeda, K., Iwakura, Y., Luheshi, G.N., Rothwell, N.J., 2003, Interleukin-18 induces expression and release of cytokines from murine glial cells: interactions with interleukin-1 beta. J Neurochem 85, 1412-1420.
163. Wiercinska-Drapalo, A., Jaroszewicz, J., Flisiak, R., Prokopowicz, D., 2004, Plasma interleukin-18 is associated with viral load and disease progression in HIV-1-infected patients. Microbes Infect 6, 1273-1277.
164. Wild, J.S., Sigounas, A., Sur, N., Siddiqui, M.S., Alam, R., Kurimoto, M., Sur, S., 2000, IFN-gamma-inducing factor (IL-18) increases allergic sensitization, serum IgE, Th2 cytokines, and airway eosinophilia in a mouse model of allergic asthma. J Immunol 164, 2701-2710.
165. Wildbaum, G., Youssef, S., Grabie, N., Karin, N., 1998, Neutralizing antibodies to IFN-gamma-inducing factor prevent experimental autoimmune encephalomyelitis. J Immunol 161, 6368-6374.
166. Wu, D., Murakami, K., Liu, N., Konishi, M., Muneta, Y., Inumaru, S., Kokuho, T., Sentsui, H., 2004, Expression of equine interleukin-18 by baculovirus expression system and its biologic activity. Microbiol Immunol 48, 471-476.
167. Xiang, Y., Moss, B., 2001, Determination of the functional epitopes of human interleukin-18-binding protein by site-directed mutagenesis. J Biol Chem 276, 17380-17386.
168. Xiang, Y., Moss, B., 2003, Molluscum contagiosum virus interleukin-18 (IL-18) binding protein is secreted as a full-length form that binds cell surface glycosaminoglycans through the C-terminal tail and a furin-cleaved form with only the IL-18 binding domain. J Virol 77, 2623-2630.
169. Xu, D., Chan, W.L., Leung, B.P., Hunter, D., Schulz, K., Carter, R.W., McInnes, I.B., Robinson, J.H., Liew, F.Y., 1998, Selective expression and functions of interleukin 18 receptor on T helper (Th) type 1 but not Th2 cells. J Exp Med 188, 1485-1492.
170. Xu, H.M., Zhang, G.Y., Ji, X.D., Cao, L., Shu, L., Hua, Z.C., 2005, Expression of soluble, biologically active recombinant human endostatin in Escherichia coli. Protein Expr Purif 41, 252-258.
171. Yamanaka, R., Tsuchiya, N., Yajima, N., Honma, J., Hasegawa, H., Tanaka, R., Ramsey, J., Blaese, R.M., Xanthopoulos, K.G., 2003, Induction of an antitumor immunological response by an intratumoral injection of dendritic cells pulsed with genetically engineered Semliki Forest virus to produce interleukin-18 combined with the systemic administration of interleukin-12. J Neurosurg 99, 746-753.
172. Yamanaka, R., Xanthopoulos, K.G., 2005, Induction of antigen-specific immune responses against malignant brain tumors by intramuscular injection of sindbis DNA encoding gp100 and IL-18. DNA Cell Biol 24, 317-324.
173. Yang, J., Murphy, T.L., Ouyang, W., Murphy, K.M., 1999, Induction of interferon-gamma production in Th1 CD4+ T cells: evidence for two distinct pathways for promoter activation. Eur J Immunol 29, 548-555.
174. Yoshimoto, T., Okamura, H., Tagawa, Y.I., Iwakura, Y., Nakanishi, K., 1997, Interleukin 18 together with interleukin 12 inhibits IgE production by induction of interferon-gamma production from activated B cells. Proc Natl Acad Sci U S A 94, 3948-3953.
175. Yoshimoto, T., Takeda, K., Tanaka, T., Ohkusu, K., Kashiwamura, S., Okamura, H., Akira, S., Nakanishi, K., 1998, IL-12 up-regulates IL-18 receptor expression on T cells, Th1 cells, and B cells: synergism with IL-18 for IFN-gamma production. J Immunol 161, 3400-3407.
176. Zecchina, G., Novick, D., Rubinstein, M., Barak, V., Dinarello, C., Nagler, A., 2001, Interleukin-18 binding protein in acute graft versus host disease and engraftment following allogeneic peripheral blood stem cell transplants. J Hematother Stem Cell Res 10, 769-776.
177. Zhai, Y., Kuick, R., Nan, B., Ota, I., Weiss, S.J., Trimble, C.L., Fearon, E.R., Cho, K.R., 2007, Gene expression analysis of preinvasive and invasive cervical squamous cell carcinomas identifies HOXC10 as a key mediator of invasion. Cancer Res 67, 10163-10172.