化学诱导剂对几种作物系统获得抗性诱导效果的研究
摘要
经研究证明，BTH是一种非常有效的植物系统性获得抗性诱导剂。
①通过不同浓度BTH对黄瓜黑星菌孢子萌发率及菌丝生长速率影响的测
试，证明0.125～2μg/gBTH对黑星菌孢子萌发及菌丝生长均无抑制作用，
因此认为经BTH处理后黄瓜幼苗病叶率的下降及诱导效果的加强是由于BTH
诱导了黄瓜对黑星病的抗性而引起的。②0.125～2μg/gBTH都可以诱导黄
瓜对黑星病产生一定程度的抗性，并且随着浓度的升高诱导效果加强。诱
导的最佳浓度为1μg/g。1μg/gBTH处理长春密刺、津研7号、津春3
号后，平均诱导效果分别为91.17％、80.94％、97.07％。③1μg/gBTH对黄
瓜黑星病的诱导效果远远高于0.1％水杨酸、0.1％苯酚、0.1％氯化钾。BTH
处理对长春密刺平均诱导效果可达83.90％，而水杨酸、苯酚、氯化钾对其
诱导效果仅分别为17.17％、32.42％、33.24％。津研7号和津春3号两个黄
瓜品种上变化趋势与长春密刺的相似。④随着BTH诱导后时间的延长，诱
导效果逐渐减弱，如1μg/gBTH处理长春密刺幼苗后，第1、2、3、4次
调查所得的诱导效果分别为100.00％、94.36％、94.50％、75.83％。经其它
浓度处理的黄瓜品种上也有上述变化趋势。⑤1μg/gBTH处理或接种病原
菌都可以诱导黄瓜及小麦叶片内多酚氧化酶（PPO）、过氧化物酶（POD）及
苯丙氨酸解氨酶（PAL）活性不同程度的升高，但BTH诱导后三种防御酶活
性的升高在时间上比接种病原菌的上升速度快，且活性程度也高。经对长
春密刺和陇鉴46号幼苗POD同工酶聚丙烯酰胺凝胶电泳，结果表明：BTH
处理或接种病原菌后，在黄瓜上可以诱导出3条新酶带，小麦上可以诱导
出2条新酶带，不论在黄瓜上还是小麦上，经BTH诱导或接种病原菌的处
理酶带颜色均较既不诱导也不接种的处理深。＠10叶 s屹BTH处理或
接种条锈菌使铭贤 169感病品种严重度和病情指数略微有所降低，160 u g
lgBTH处理后，严重度从对照的 33．33o降至 M ．42o，病情指数从对照的
引．25％降至 32．22％，诱导效果为 42．76％。同样浓度的 BTH处理小麦白粉
菌高感品种陇鉴 46号后，病叶率和严重度均有所降低， 10 u g乍和 160 u g
lgBTH处理后，严重度分别从对照的34．87％降至26．25％和5．ZI％，病叶率
分别从对照的100．00o降为93．75o和77．22o。
Up to date, it has been proved that BTH can effectively induce SAR in
plants to pathogens. In this research, the investigated results are as follows:
0.125 ig/gBTH had no significant effect on germination of conidia and
mycelium growth of Cladosporium cucumerinum ,so the reason why the rate of
disease plants and leaves reduced was mainly because of the BTH-induced
resistance.
BTH at different concentrations used in the experiment can induce some degree
of resistance to C.cucumeirinum, and the effect of induced resistance was.
increased with the increase of BTH concentrations, but the optimum
concentration was lii g 1g. After being pretreated by I ii g IgBTH,the average
effect of induced resistance in Changchunmici, Jinyan7 and Jinchun3 was
91.17%, 80.9% and 97.07%,respectively.
0.125 igIgBTH-induced resistance to C.cucumeirinum reduced gradually
with the time development, for example, the observed data at the 1 st,2nd,3rd
and 4th time was 100.00%, 94.36%, 94.50% and 75.83%,respectively.
I i g/gBTH-induced resistance in cucumber seedlings was far stronger than that
of 0.l%Salicylic Acid,0.l%Phenol and 0.l%KC1-induced,the average effect of
BTH-treated resistance in Changchunmici seedlings was 83.90%,but after being
treated by Salicylic Acid, Phenol and KC1, the average effect was only
17.1 7%,32.42% and 33 .24%,respectively.
All peroxidase(POD),polyphenoloxidase(PPO) and phenylanine ammo-
nialyase(PAL) activities in cucumber and wheat were increased differently after
being treated by BTH or inoculation with pathogens, but BTH-treated enzyme
activities were increased quickly and highly than those pathogen-inoculated.
The isozyme patterns of POD on polyacrylainde gels showed that there was 2
novel isozymes induced by either BTH or C.cucutnerinutn in cucumber,in
wheat, 3 novel isozymes induced, what’s more, the BTH-induced or
pathogens-inoculated isozyme colour was more darker than that of neither
BTH-treated nor pathogen-inoculated.
10-460 ii gIgBTH gave weak protection to susceptible variety of wheat-
Mingxianl69 against Puccinia f.sp.tritici,l60 ig/gBTH-treated disease severity
was reduced from 33.3 3% in the controls to 11 .42%,disease index was reduced
from 51.25% in the controls to 3 2.22%.
ft Treatment of susceptible variety of wheat-Longjian 46 by 10-460 g/gBTH
also had some degree of effect on infection of Blutneria f.sp.fritici, eg.,
pretreatment by 10 or 160 t g/gBTH ,the rate of disease leaves was reduced
from 34.87% in the controls to 26.25% and 5.2 l%,the rate of disease leaves was
reduced from 100.00% in the controls to 93.75% and 77.22%,respectively.
①通过不同浓度BTH对黄瓜黑星菌孢子萌发率及菌丝生长速率影响的测
试，证明0.125～2μg/gBTH对黑星菌孢子萌发及菌丝生长均无抑制作用，
因此认为经BTH处理后黄瓜幼苗病叶率的下降及诱导效果的加强是由于BTH
诱导了黄瓜对黑星病的抗性而引起的。②0.125～2μg/gBTH都可以诱导黄
瓜对黑星病产生一定程度的抗性，并且随着浓度的升高诱导效果加强。诱
导的最佳浓度为1μg/g。1μg/gBTH处理长春密刺、津研7号、津春3
号后，平均诱导效果分别为91.17％、80.94％、97.07％。③1μg/gBTH对黄
瓜黑星病的诱导效果远远高于0.1％水杨酸、0.1％苯酚、0.1％氯化钾。BTH
处理对长春密刺平均诱导效果可达83.90％，而水杨酸、苯酚、氯化钾对其
诱导效果仅分别为17.17％、32.42％、33.24％。津研7号和津春3号两个黄
瓜品种上变化趋势与长春密刺的相似。④随着BTH诱导后时间的延长，诱
导效果逐渐减弱，如1μg/gBTH处理长春密刺幼苗后，第1、2、3、4次
调查所得的诱导效果分别为100.00％、94.36％、94.50％、75.83％。经其它
浓度处理的黄瓜品种上也有上述变化趋势。⑤1μg/gBTH处理或接种病原
菌都可以诱导黄瓜及小麦叶片内多酚氧化酶（PPO）、过氧化物酶（POD）及
苯丙氨酸解氨酶（PAL）活性不同程度的升高，但BTH诱导后三种防御酶活
性的升高在时间上比接种病原菌的上升速度快，且活性程度也高。经对长
春密刺和陇鉴46号幼苗POD同工酶聚丙烯酰胺凝胶电泳，结果表明：BTH
处理或接种病原菌后，在黄瓜上可以诱导出3条新酶带，小麦上可以诱导
出2条新酶带，不论在黄瓜上还是小麦上，经BTH诱导或接种病原菌的处
理酶带颜色均较既不诱导也不接种的处理深。＠10叶 s屹BTH处理或
接种条锈菌使铭贤 169感病品种严重度和病情指数略微有所降低，160 u g
lgBTH处理后，严重度从对照的 33．33o降至 M ．42o，病情指数从对照的
引．25％降至 32．22％，诱导效果为 42．76％。同样浓度的 BTH处理小麦白粉
菌高感品种陇鉴 46号后，病叶率和严重度均有所降低， 10 u g乍和 160 u g
lgBTH处理后，严重度分别从对照的34．87％降至26．25％和5．ZI％，病叶率
分别从对照的100．00o降为93．75o和77．22o。
Up to date, it has been proved that BTH can effectively induce SAR in
plants to pathogens. In this research, the investigated results are as follows:
0.125 ig/gBTH had no significant effect on germination of conidia and
mycelium growth of Cladosporium cucumerinum ,so the reason why the rate of
disease plants and leaves reduced was mainly because of the BTH-induced
resistance.
BTH at different concentrations used in the experiment can induce some degree
of resistance to C.cucumeirinum, and the effect of induced resistance was.
increased with the increase of BTH concentrations, but the optimum
concentration was lii g 1g. After being pretreated by I ii g IgBTH,the average
effect of induced resistance in Changchunmici, Jinyan7 and Jinchun3 was
91.17%, 80.9% and 97.07%,respectively.
0.125 igIgBTH-induced resistance to C.cucumeirinum reduced gradually
with the time development, for example, the observed data at the 1 st,2nd,3rd
and 4th time was 100.00%, 94.36%, 94.50% and 75.83%,respectively.
I i g/gBTH-induced resistance in cucumber seedlings was far stronger than that
of 0.l%Salicylic Acid,0.l%Phenol and 0.l%KC1-induced,the average effect of
BTH-treated resistance in Changchunmici seedlings was 83.90%,but after being
treated by Salicylic Acid, Phenol and KC1, the average effect was only
17.1 7%,32.42% and 33 .24%,respectively.
All peroxidase(POD),polyphenoloxidase(PPO) and phenylanine ammo-
nialyase(PAL) activities in cucumber and wheat were increased differently after
being treated by BTH or inoculation with pathogens, but BTH-treated enzyme
activities were increased quickly and highly than those pathogen-inoculated.
The isozyme patterns of POD on polyacrylainde gels showed that there was 2
novel isozymes induced by either BTH or C.cucutnerinutn in cucumber,in
wheat, 3 novel isozymes induced, what’s more, the BTH-induced or
pathogens-inoculated isozyme colour was more darker than that of neither
BTH-treated nor pathogen-inoculated.
10-460 ii gIgBTH gave weak protection to susceptible variety of wheat-
Mingxianl69 against Puccinia f.sp.tritici,l60 ig/gBTH-treated disease severity
was reduced from 33.3 3% in the controls to 11 .42%,disease index was reduced
from 51.25% in the controls to 3 2.22%.
ft Treatment of susceptible variety of wheat-Longjian 46 by 10-460 g/gBTH
also had some degree of effect on infection of Blutneria f.sp.fritici, eg.,
pretreatment by 10 or 160 t g/gBTH ,the rate of disease leaves was reduced
from 34.87% in the controls to 26.25% and 5.2 l%,the rate of disease leaves was
reduced from 100.00% in the controls to 93.75% and 77.22%,respectively.
引文
1 袁美丽，1991．黄瓜黑星病侵染和发病规律及其生态防治的研究，植物保护学报18(3)：273-278
2 王生荣，1995。我省黄瓜黑星病发生规律及防治技术 甘肃农业科技10(238)：36-38
3 张满良主编 1997．农业植物病理学 北京：世界图书出版公司
4 张德水，1997，植物病理学报27(2)：97-103
5 Schlosser, K Systemic Activated Resistance—a New Dimensionin Plant Protection Arab. J. PI.15 (2): 147-149
6 Ross, A.F. 1961. Systemic acquired resistance induced by localized virus infections in plant. Virology 14:340-358
7 Caruso, F. and Kue, J.1997. Field of Protection of cucumber, Watermellon and Muskmelon against Colletotrichum lagenarium Phytopathology 67:1209-1292
8 刘爱新，粱元存，张博，商明清，1998，植物诱导抗性研究进展 植物病理学报29(3)：410－413
9 Ye,X.S.et al.1989.烟草花叶病毒、烟草霜霉病毒和阿司匹林由于诱导病程相关蛋白以及烟草对霜霉病与烟草花叶病毒的系统性抗性[英]．Physiological and Molecular Plant Pathlolgy 35:61-175
10 Ye,X.S.et al.1991.烟草对霜霉病及烟草花叶病毒系统抗性有关的过氧化物酶活性、同工酶谱和细胞定位[英]。Phytopathology 80(12)：11295-1299
11 Ajlan AM,Potter DA,1992.Lack of effect of mosaic virus-induced systemic acquired resistance to anthropod herbivores in tobacco.Phytopatbology 82:647-651
12 Maurhofer,M,hase,C., Meuwly, P. et al. 1994. Inducion of Systemic Resistance of Tobacco to Necrosis Virus by the Root Colonizing Pseudomonas fluorescens Strain CHAO: Influence of the gacA Gene and of Pyoverdine Production. Phytopathology 84 (2): 139-146
13 ANFOKA,G.,Buchenauer, H 1997. Induction of systemic resistance in tomato and tobacco plants against cucumber mosaic virus Journal of Plant Diseases and Protection 104(5): 506-516
14 MILLS,P.R. md WOOD, R.K.S.1984.The effects of polyacrylic acid, acetylsalicylic acid and salicylic acid on resistance of cucumber to Colletotrichum lagenarium Phytopath. Z..209-216
15 Mucharomah E. et al. 1991. Crop Protection 10:265-270
16 HIWEGEN,T. and VERHAAR,M.A.1994. Effects of cucumber genotype on the induction of resistance to powdery mildew,Sphaerotheca fuliginea by 2,6-dichloroisonicotinic acid.Plant Pathology 44, 756-762
17 zhang, W., Dick. W. A. et al. 1996. Compost-induced systemic acquired resistance in cucumber to pythium root rot and anthracnos. Pytopathology 86(10): 1066-1070
18 Kuc J, Richmond -S, 1977. Aspects of the Protection of cucumber sgainst Colletotrichum lagenarium by Colletotrichum lagenarium.Phytopathology 67:533-536
19 KATARIA, H. R. et al. 1997. Efficacy of resistance inducers, free-radicalscavengers and an antagonistic strain of Pseudomonas fluorescens for control of Rhizoctonia solani AG-4 in bean and cucumber. Plant Pathology 46,897-909
20 Pennazio, S....1991.长豇豆对病毒侵染的系统获得抗性与乙烯的生物合成[英]. Journal of phytopathology 131 (3): 177-183
21 CHIN.K.M.,WOLFE,M. S. et al. 1984.Host-mediated interactions between pathogen genotypes.Plant Pathology 33, 161-171
22 Smith J.,M(?)traux J-P. 1991.Pseudomonas syringae induces systemic resistance to Pyricularia orzyzae in rice. Physiol.Mol. Plant Pathol. 39:451-461
23 MARTINLLI, J.A,BROWN,J.K.M. et al. 1993. Effects of barley genotype on induced resistance To powdery midew. Plant Pathology 42, 195-202
24 Karl-Heinz Kogel, ULi Beckhove, et al., 1994. Acquired resistance in barley-zhe resistance mechanism induced by 2,6-dichloroisonicotinic acid is a phenocopy of a genetically based mechanism governing race-specific powdery midew resistance. Plant Physiol. 106:1269-1277
25 Lyngs Jorgensen.H.J.,Andresen,H.et al. 1996. Control of Drechslera teres and Septoria nodorum Phytopathology 86:602-607
26 Lyngs Jorgensen, H.J.,Stephensen L(?)bek, P. et al. 199K Mechanisms of induced resistance in barley against Drechslera teres.Phytopathology 88:698-707
27 Gorlach J.et al.1996.一种新的系统性得抗性诱导剂-苯丙噻重氮(BTH)，可以激发小麦SAR基因的表达[英]．Plant Cell 8(4)：629-643
28 张元恩，1989．诱导黄瓜系统抗病性研究，北京农业大学学报15(1)：62－65
29 张元恩，刘英慧，1992，非杀菌剂化合物防治瓜类病害的研究，植物病理学报22(3)
30 李洪连，王守正等，1993．黄瓜对炭疽病诱导抗性的初步研究。植物病理学报23(4)：327－332
31 云兴福等，1997．几种化学物质诱导黄瓜对霜霉病的抗性，植物保护学报24(2)：159－163
32 骆桂芬等，1997．黄瓜叶片中糖和木质素含量与霜霉病诱导抗性的关系，植物病理学报27(1)：65－69
33 骆桂芬等。1997．东北霜霉菌对黄瓜霜霉病的诱导免疫作用。植物病理学报26(2)：359－364
34 张宗申等，1998．非生物诱抗剂草酸对黄瓜叶片中过氧化物酶的系统诱导作用．植物病理学报28(1)：145-150
35 王国平，罗宽，1994．水稻品种抗瘟性诱导研究：1．非生物诱导物的筛选及诱导方法初探．植物病理学报24(2)：123－127
36 吴岳轩，曾富华，王荣臣，1996，杂交稻对白叶枯病的诱导抗性与细胞内防御酶系统关系的初步研究．植物病理学报26(2)：127-131
37 蔡新忠．郑重，宋凤鸣，1996．水杨酸对水稻幼苗抗瘟性的诱导作用．植物病理学报26(1)7-12
38 蔡新忠，郑重，1997．水杨酸诱导水稻幼苗抗瘟性的生化机制．植物病理学报27(3)：231-236
39 朱建祥，1998．小麦对白粉菌的抗性和感病性的诱导．植物病理学报28(1)：11-17
40 Leeman,M., Van Pett, J. K et al. 1995. Induction of systemic resistance against Fusarium wilt of radish by lipopolysaccharides of Pseudomonas fluorescens. Phytopathology 85(9):1021-1027
41 Helmut Kessman, Theo Staub, et al. 1994. Induction of systemic acquired disease resistance in plants by chemicals. Annu. Rev. Phytopathol. 32:439-459
42 Enyedi AJ, Paskin I.1993. Induction of UDP-glucose: SA glucosyl-transferase activity in mosaic virus-inoculated tobacco leaves. Plant Physiol. 101:1375-1380
43 HIJWEGEN, T. et al. 1996. Resistance to Sphaerotheca pannosa in roses induced by INA.Plant Pathology 45, 631-635
44 余迪逑，岑川，李宝健，傅家瑞，1993，植物系统获得抗病性和信号传导．植物学报41(2)：115－124
45 Sticher, L, Mauch-Mani,B.et al. 1997.Systemic acquired resistance. Annu.Rev.Phytopathol.35:235-270
46 蔡新忠，郑重1999．植物系统性获得抗性的产生机理和途径．植物保护学报26(1)：83-90盼仞
47 方中达，1998．植病研究法(第三版) 北京：中国农业出版社
48 朱建兰，陈秀蓉，1998．黄瓜品种对黑星病的抗性鉴定结果．甘肃农业科技7，32-33
49 汪沛洪主编，1985．基础生物化学实验指导．陕西科学技术出版社
50 李靖等，1991．黄瓜感染霜霉病叶片中一些酶活性的变化，植物病理学报21(4)：277—282
51 秦鑫，李忠恕，1985．兰州大学学报(自然科学版)21(1)：61－64
52 商鸿生主编，1997．植物免疫学实验 中国农业出版社
53 欧阳光察，薛应龙等，1988，植物生理学通讯24：9－16
54 Mayer, A.M.et al. 1979. Phytochem 18:193-215
55 铃木直治等箸，张际中等译，1981，近代植物病理学化学 中海科学出版社
2 王生荣，1995。我省黄瓜黑星病发生规律及防治技术 甘肃农业科技10(238)：36-38
3 张满良主编 1997．农业植物病理学 北京：世界图书出版公司
4 张德水，1997，植物病理学报27(2)：97-103
5 Schlosser, K Systemic Activated Resistance—a New Dimensionin Plant Protection Arab. J. PI.15 (2): 147-149
6 Ross, A.F. 1961. Systemic acquired resistance induced by localized virus infections in plant. Virology 14:340-358
7 Caruso, F. and Kue, J.1997. Field of Protection of cucumber, Watermellon and Muskmelon against Colletotrichum lagenarium Phytopathology 67:1209-1292
8 刘爱新，粱元存，张博，商明清，1998，植物诱导抗性研究进展 植物病理学报29(3)：410－413
9 Ye,X.S.et al.1989.烟草花叶病毒、烟草霜霉病毒和阿司匹林由于诱导病程相关蛋白以及烟草对霜霉病与烟草花叶病毒的系统性抗性[英]．Physiological and Molecular Plant Pathlolgy 35:61-175
10 Ye,X.S.et al.1991.烟草对霜霉病及烟草花叶病毒系统抗性有关的过氧化物酶活性、同工酶谱和细胞定位[英]。Phytopathology 80(12)：11295-1299
11 Ajlan AM,Potter DA,1992.Lack of effect of mosaic virus-induced systemic acquired resistance to anthropod herbivores in tobacco.Phytopatbology 82:647-651
12 Maurhofer,M,hase,C., Meuwly, P. et al. 1994. Inducion of Systemic Resistance of Tobacco to Necrosis Virus by the Root Colonizing Pseudomonas fluorescens Strain CHAO: Influence of the gacA Gene and of Pyoverdine Production. Phytopathology 84 (2): 139-146
13 ANFOKA,G.,Buchenauer, H 1997. Induction of systemic resistance in tomato and tobacco plants against cucumber mosaic virus Journal of Plant Diseases and Protection 104(5): 506-516
14 MILLS,P.R. md WOOD, R.K.S.1984.The effects of polyacrylic acid, acetylsalicylic acid and salicylic acid on resistance of cucumber to Colletotrichum lagenarium Phytopath. Z..209-216
15 Mucharomah E. et al. 1991. Crop Protection 10:265-270
16 HIWEGEN,T. and VERHAAR,M.A.1994. Effects of cucumber genotype on the induction of resistance to powdery mildew,Sphaerotheca fuliginea by 2,6-dichloroisonicotinic acid.Plant Pathology 44, 756-762
17 zhang, W., Dick. W. A. et al. 1996. Compost-induced systemic acquired resistance in cucumber to pythium root rot and anthracnos. Pytopathology 86(10): 1066-1070
18 Kuc J, Richmond -S, 1977. Aspects of the Protection of cucumber sgainst Colletotrichum lagenarium by Colletotrichum lagenarium.Phytopathology 67:533-536
19 KATARIA, H. R. et al. 1997. Efficacy of resistance inducers, free-radicalscavengers and an antagonistic strain of Pseudomonas fluorescens for control of Rhizoctonia solani AG-4 in bean and cucumber. Plant Pathology 46,897-909
20 Pennazio, S....1991.长豇豆对病毒侵染的系统获得抗性与乙烯的生物合成[英]. Journal of phytopathology 131 (3): 177-183
21 CHIN.K.M.,WOLFE,M. S. et al. 1984.Host-mediated interactions between pathogen genotypes.Plant Pathology 33, 161-171
22 Smith J.,M(?)traux J-P. 1991.Pseudomonas syringae induces systemic resistance to Pyricularia orzyzae in rice. Physiol.Mol. Plant Pathol. 39:451-461
23 MARTINLLI, J.A,BROWN,J.K.M. et al. 1993. Effects of barley genotype on induced resistance To powdery midew. Plant Pathology 42, 195-202
24 Karl-Heinz Kogel, ULi Beckhove, et al., 1994. Acquired resistance in barley-zhe resistance mechanism induced by 2,6-dichloroisonicotinic acid is a phenocopy of a genetically based mechanism governing race-specific powdery midew resistance. Plant Physiol. 106:1269-1277
25 Lyngs Jorgensen.H.J.,Andresen,H.et al. 1996. Control of Drechslera teres and Septoria nodorum Phytopathology 86:602-607
26 Lyngs Jorgensen, H.J.,Stephensen L(?)bek, P. et al. 199K Mechanisms of induced resistance in barley against Drechslera teres.Phytopathology 88:698-707
27 Gorlach J.et al.1996.一种新的系统性得抗性诱导剂-苯丙噻重氮(BTH)，可以激发小麦SAR基因的表达[英]．Plant Cell 8(4)：629-643
28 张元恩，1989．诱导黄瓜系统抗病性研究，北京农业大学学报15(1)：62－65
29 张元恩，刘英慧，1992，非杀菌剂化合物防治瓜类病害的研究，植物病理学报22(3)
30 李洪连，王守正等，1993．黄瓜对炭疽病诱导抗性的初步研究。植物病理学报23(4)：327－332
31 云兴福等，1997．几种化学物质诱导黄瓜对霜霉病的抗性，植物保护学报24(2)：159－163
32 骆桂芬等，1997．黄瓜叶片中糖和木质素含量与霜霉病诱导抗性的关系，植物病理学报27(1)：65－69
33 骆桂芬等。1997．东北霜霉菌对黄瓜霜霉病的诱导免疫作用。植物病理学报26(2)：359－364
34 张宗申等，1998．非生物诱抗剂草酸对黄瓜叶片中过氧化物酶的系统诱导作用．植物病理学报28(1)：145-150
35 王国平，罗宽，1994．水稻品种抗瘟性诱导研究：1．非生物诱导物的筛选及诱导方法初探．植物病理学报24(2)：123－127
36 吴岳轩，曾富华，王荣臣，1996，杂交稻对白叶枯病的诱导抗性与细胞内防御酶系统关系的初步研究．植物病理学报26(2)：127-131
37 蔡新忠．郑重，宋凤鸣，1996．水杨酸对水稻幼苗抗瘟性的诱导作用．植物病理学报26(1)7-12
38 蔡新忠，郑重，1997．水杨酸诱导水稻幼苗抗瘟性的生化机制．植物病理学报27(3)：231-236
39 朱建祥，1998．小麦对白粉菌的抗性和感病性的诱导．植物病理学报28(1)：11-17
40 Leeman,M., Van Pett, J. K et al. 1995. Induction of systemic resistance against Fusarium wilt of radish by lipopolysaccharides of Pseudomonas fluorescens. Phytopathology 85(9):1021-1027
41 Helmut Kessman, Theo Staub, et al. 1994. Induction of systemic acquired disease resistance in plants by chemicals. Annu. Rev. Phytopathol. 32:439-459
42 Enyedi AJ, Paskin I.1993. Induction of UDP-glucose: SA glucosyl-transferase activity in mosaic virus-inoculated tobacco leaves. Plant Physiol. 101:1375-1380
43 HIJWEGEN, T. et al. 1996. Resistance to Sphaerotheca pannosa in roses induced by INA.Plant Pathology 45, 631-635
44 余迪逑，岑川，李宝健，傅家瑞，1993，植物系统获得抗病性和信号传导．植物学报41(2)：115－124
45 Sticher, L, Mauch-Mani,B.et al. 1997.Systemic acquired resistance. Annu.Rev.Phytopathol.35:235-270
46 蔡新忠，郑重1999．植物系统性获得抗性的产生机理和途径．植物保护学报26(1)：83-90盼仞
47 方中达，1998．植病研究法(第三版) 北京：中国农业出版社
48 朱建兰，陈秀蓉，1998．黄瓜品种对黑星病的抗性鉴定结果．甘肃农业科技7，32-33
49 汪沛洪主编，1985．基础生物化学实验指导．陕西科学技术出版社
50 李靖等，1991．黄瓜感染霜霉病叶片中一些酶活性的变化，植物病理学报21(4)：277—282
51 秦鑫，李忠恕，1985．兰州大学学报(自然科学版)21(1)：61－64
52 商鸿生主编，1997．植物免疫学实验 中国农业出版社
53 欧阳光察，薛应龙等，1988，植物生理学通讯24：9－16
54 Mayer, A.M.et al. 1979. Phytochem 18:193-215
55 铃木直治等箸，张际中等译，1981，近代植物病理学化学 中海科学出版社