福建山樱花组织培养技术研究
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摘要
福建山樱花是著名的木本观赏花卉,近年来,福建山樱花栽培如火如荼,苗木供不应求。本试验以从福建省不同产地采集的3个不同种源FS01、FS02、FS03福建山樱花嫩枝为基本材料,从无菌体系的建立、继代培养、生根诱导、炼苗移栽等各个环节展开快繁技术体系研究,并分析在培养过程中污染率高和腋芽诱导率低等关键因素,为实现福建山樱花工厂化育苗提供必要的理论基础和技术支撑。主要研究结果如下:
1.福建山樱花无菌体系的建立。分别使用升汞、次氯酸钠对外植体进行消毒,筛选出比较理想的消毒方法是,用70%乙醇浸泡10s,然后用0.1%升汞加吐温2滴约0.01ml浸泡6 min,可以使污染率控制在36.7%,成活率达到45%。
2.福建山樱花腋芽诱导。采用正交试验设计,筛选出适合种源FS01的腋芽诱导培养基为1/2MS+4.0mg/L BA 0.025mg/L IBA+1.0g/L AC,萌芽率达87.5%;适合种源FS02的腋芽诱导培养基为1/2MS+2.0mg/L BA+0.05mg/L IBA+1.2g/LAC,萌芽率达84.6%;适合种源FS03的腋芽诱导培养基为1/2MS+3.0mg/L BA+0.075mg/LIBA+0.8g/LAC和1/2MS+4.0mg/L BA+0.025mg/L IBA+1.0g/L AC,萌芽率达80.0%。
3.福建山樱花增殖培养。采用正交试验设计,筛选出适合种源FS01的增殖培养基为MS+1.0mg/L BA+0.15mg/L IBA+1.0mg/L GA_3,30d后增殖数达5.20;适合种源FS02的增殖培养基为MS+1.0mg/L BA+0.03mg/L IBA+0.1mg/L GA_3和MS+1.0mg/L BA+0.08mg/L IBA+1.0mg/L GA_3,30d后增殖数达5.00;适合种源FS03的增殖培养基为MS+2.0mg/L BA+0.05mg/L IBA+1.0mg/L GA_3,30d后增殖数达6.00。
4.福建山樱花生根培养。采用正交试验设计,筛选适合种源FS01、FS02、PS03的生根培养基均为1/2MS+1.0mg/L IBA+1.0mg/L NAA,30d后生根率分别为90.6%、90.0%、91.7%。
5.福建山樱花炼苗与移栽。筛选出适合福建山樱花移栽基质为黄心土:蛭石=1:1,成活率达75.0%;比较适宜移栽的时间为11月份,成活率达76.7%;最佳移栽环境为全光雾大棚,成活率达76.0%。
Prunus spp.is the famous woody appreciates flower.In recent years,the flower cultivation is in imposing array and the seedling wood supply falls short of demand.In this experiment,chose three different provenance of Prunus campanulata from different habitats in Fujian Province as basic material,researched about every steps of the establishment of aseptic system,proliferation,root induction,transplantation etc. Analyzed the key factors of pollution and rootage rate,provided the theoretical basic for the industrial seedling of Prunus campanulata.The main results were described as follows:
The aseptic system was established.Sanitized by HgCl_2 or NaCIO and sieved the better method of disinfection is that soak 10 seconds by 70%alcohol,then soak 6 minutes by 0.1%HgCl_2.Then the pollution rate was 36.7%and the survive rate was 45%.
Induce the axial bud of Prunus campanulata.Use orthogonal design,then sieved the better scheme of the media for the axial bud induction of P.campanulata FS01 is 1/2MS+4.0mg/L BA 0.025mg/L IBA+1.0g/L AC,and the embryonic rate is 87.5%;the better scheme of the media for the axial bud induction on P.campanulata FS02 is 1/2MS+2.0mg/L BA+0.05mg/L IBA+1.2g/LAC,and the embryonic rate is 84.6%;the better scheme of the media for the axial bud induction on P.campanulata FS03 is 1/2MS+3.0mg/L BA+0.075mg/LIBA+0.8g/LAC or 1/2MS+4.0mg/L BA+0.025mg/L IBA+I.0g/LAC,and the embryonic rate is 80.0%.
The proliferation of Prunus campanulata.Use orthogonal design,then sieved the better scheme of the media for the proliferation of P.campanulata FS01 is MS+1.0mg/L BA+0.15mg/L IBA+1.0mg/L GA_3,and the number of proliferation is 5.20 after 30 days; the better scheme of the media for the proliferation of P.campanulata FS02 is MS+1.0mg/L BA+0.03mg/L IBA+0.1mg/L GA3 or MS+1.0mg/L BA+0.08mg/L IBA+1.0mg/L GA_3,and the number of proliferation is 5.00 after 30 days;the better scheme of the media for the proliferation of P.campanulata FS03 is MS+2.0mg/L BA +0.05mg/L IBA+1.0mg/L GA_3,and the number of proliferation is 6.00 after 30 days.
The rootage of Prunus campanulata.Use orthogonal design,then sieved the better scheme of the media for the rootage of P.campanulata FS01、FS02、FS03 is 1/2MS+1.0mg/L IBA+1.0mg/L NAA,and the rootage rate is 90.6%,90.0%,91.7%after 30 days.
The transplantation of Prunus campanulata.The better transplantation medium is ocher:vermiculite=1:1 and the survive rate is 75%;The better transplantation time is November and the survive rate is 76.7%;The better transplantation environment is the shed with full mist and the survive rate can reach 76.0%.
1.福建山樱花无菌体系的建立。分别使用升汞、次氯酸钠对外植体进行消毒,筛选出比较理想的消毒方法是,用70%乙醇浸泡10s,然后用0.1%升汞加吐温2滴约0.01ml浸泡6 min,可以使污染率控制在36.7%,成活率达到45%。
2.福建山樱花腋芽诱导。采用正交试验设计,筛选出适合种源FS01的腋芽诱导培养基为1/2MS+4.0mg/L BA 0.025mg/L IBA+1.0g/L AC,萌芽率达87.5%;适合种源FS02的腋芽诱导培养基为1/2MS+2.0mg/L BA+0.05mg/L IBA+1.2g/LAC,萌芽率达84.6%;适合种源FS03的腋芽诱导培养基为1/2MS+3.0mg/L BA+0.075mg/LIBA+0.8g/LAC和1/2MS+4.0mg/L BA+0.025mg/L IBA+1.0g/L AC,萌芽率达80.0%。
3.福建山樱花增殖培养。采用正交试验设计,筛选出适合种源FS01的增殖培养基为MS+1.0mg/L BA+0.15mg/L IBA+1.0mg/L GA_3,30d后增殖数达5.20;适合种源FS02的增殖培养基为MS+1.0mg/L BA+0.03mg/L IBA+0.1mg/L GA_3和MS+1.0mg/L BA+0.08mg/L IBA+1.0mg/L GA_3,30d后增殖数达5.00;适合种源FS03的增殖培养基为MS+2.0mg/L BA+0.05mg/L IBA+1.0mg/L GA_3,30d后增殖数达6.00。
4.福建山樱花生根培养。采用正交试验设计,筛选适合种源FS01、FS02、PS03的生根培养基均为1/2MS+1.0mg/L IBA+1.0mg/L NAA,30d后生根率分别为90.6%、90.0%、91.7%。
5.福建山樱花炼苗与移栽。筛选出适合福建山樱花移栽基质为黄心土:蛭石=1:1,成活率达75.0%;比较适宜移栽的时间为11月份,成活率达76.7%;最佳移栽环境为全光雾大棚,成活率达76.0%。
Prunus spp.is the famous woody appreciates flower.In recent years,the flower cultivation is in imposing array and the seedling wood supply falls short of demand.In this experiment,chose three different provenance of Prunus campanulata from different habitats in Fujian Province as basic material,researched about every steps of the establishment of aseptic system,proliferation,root induction,transplantation etc. Analyzed the key factors of pollution and rootage rate,provided the theoretical basic for the industrial seedling of Prunus campanulata.The main results were described as follows:
The aseptic system was established.Sanitized by HgCl_2 or NaCIO and sieved the better method of disinfection is that soak 10 seconds by 70%alcohol,then soak 6 minutes by 0.1%HgCl_2.Then the pollution rate was 36.7%and the survive rate was 45%.
Induce the axial bud of Prunus campanulata.Use orthogonal design,then sieved the better scheme of the media for the axial bud induction of P.campanulata FS01 is 1/2MS+4.0mg/L BA 0.025mg/L IBA+1.0g/L AC,and the embryonic rate is 87.5%;the better scheme of the media for the axial bud induction on P.campanulata FS02 is 1/2MS+2.0mg/L BA+0.05mg/L IBA+1.2g/LAC,and the embryonic rate is 84.6%;the better scheme of the media for the axial bud induction on P.campanulata FS03 is 1/2MS+3.0mg/L BA+0.075mg/LIBA+0.8g/LAC or 1/2MS+4.0mg/L BA+0.025mg/L IBA+I.0g/LAC,and the embryonic rate is 80.0%.
The proliferation of Prunus campanulata.Use orthogonal design,then sieved the better scheme of the media for the proliferation of P.campanulata FS01 is MS+1.0mg/L BA+0.15mg/L IBA+1.0mg/L GA_3,and the number of proliferation is 5.20 after 30 days; the better scheme of the media for the proliferation of P.campanulata FS02 is MS+1.0mg/L BA+0.03mg/L IBA+0.1mg/L GA3 or MS+1.0mg/L BA+0.08mg/L IBA+1.0mg/L GA_3,and the number of proliferation is 5.00 after 30 days;the better scheme of the media for the proliferation of P.campanulata FS03 is MS+2.0mg/L BA +0.05mg/L IBA+1.0mg/L GA_3,and the number of proliferation is 6.00 after 30 days.
The rootage of Prunus campanulata.Use orthogonal design,then sieved the better scheme of the media for the rootage of P.campanulata FS01、FS02、FS03 is 1/2MS+1.0mg/L IBA+1.0mg/L NAA,and the rootage rate is 90.6%,90.0%,91.7%after 30 days.
The transplantation of Prunus campanulata.The better transplantation medium is ocher:vermiculite=1:1 and the survive rate is 75%;The better transplantation time is November and the survive rate is 76.7%;The better transplantation environment is the shed with full mist and the survive rate can reach 76.0%.
引文
[1]王信.樱花[J].中国花卉园艺,2002(8):38.
[2]黄雯.中国古代花卉文献研究[D].西北农林科技大学,2003:9.
[3]尚礼.阳春樱花正烂漫[J].中国花卉园艺,2003(7):46.
[4]王铖,朱红霞,周汉其.我国冬季开花植物资源研究进展[J].河北林业科技,2007(3):29-32.
[5]朱红霞,王铖.我国冬季开花植物资源及园林景观营造研究[J].山东林业科技,2006(6):71-74.
[6]陈玲.家门前的樱花树[J].中国花卉园艺,2002(7):45.
[7]冯怀字.樱花——日本人心灵的故乡[J].少年科技博览,2003(Z2):8-9.
[8]王相飞.中日“樱花”意象比较研究[J].南京师范大学文学院学报,2007(2):112-118.
[9]朱荣根,赵瑞云.日本人与樱花[J].知识就是力量,2001(4):32.
[10]闵崇博[日].绮丽的樱花[J].中国园林,2004(6):66-67.
[11]张晋岩[日].留住那场樱花雨——日本樱花面面观[J].中国花卉盆景,2003(5):25-26.
[12]Byun-K- O,Park -H- S,Lee- S-K.Eeffect of position of explants on rooting rate and rot development of Prunus serrulata.Research Report of the Forest Genetics Research Institute,1995(31):106-111.
[13]Baranova M.Principles of comparative stomatographic studies of flowering plants.Botanical Review,1992.58(1):49-92.
[14]老实人.武汉浪漫樱花节——武大赏樱[J].粤港澳市场与价格,2006(4):46-47.
[15]王贤荣,黄国富.中国樱花类植物资源及其开发利用[J].林业科技开发,2001(6):3-5.
[16]段晓梅,樱花繁殖综述[J].思茅师范高等专科学校学报,2002,18(3):82-85.
[17]王艳华,高述民,李凤兰等.大山樱种子休眠机理的探讨[J].种子,2005,24(5):12-16.
[18]Ching-Te Chien,Shun-Ying Chen,Jeng-Chuan Yang.Effect of Stratification and Drying on the Germination and Storage of Prunus campanulata Seeds[J].Taiwan Journal of Forest Science,2002,17(4):413-420.
[19]Shun-Ying Chen,Ching-Te Chien,Jeng-Der Chung etc.Dormancy-break and germination in seeds of Prunus campanulata(Rosaceae):role of covering layers and changes in concentration of abscisic acid and gibberellins[J].Seed Science Research,2007(17):21-32.
[20]王贤荣,黄国富.中国樱花类植物资源及其开发利用[J].林业科技开发,2001,15(6):3-6.
[21]张义,王剑峰.赤霉素浸泡与层积时间对山樱种子萌发的影响[J].长江大学学报(自然科学版),2005,2(8):26-27.
[22]方妙辉.钟花樱桃种子冷处理及生根剂浸种试验[J].福建果树,2006(3):52-53.
[23]徐兆波,郭绍霞.垂枝樱花引种观察与繁育技术研究[J].莱阳农学院学报,2001(1):32-36.
[24]汪雪文.嫁接培育日本樱花大苗技术[J].安徽林业科技,2006(3):29-30.
[25]孙业文.樱花嫁接育苗技术[J].江苏林业科技,2002,29(3):43.
[26]张艳芳.樱花的切接繁殖[J].花木盆景:花卉园艺,2006(2):14-15.
[27]王永清,汤浩茹.几种木本园艺植物塑料袋内密封扦插试验[J].四川农业大学,1997,15(4):486-490.
[28]陆贵巧,林青.樱花荫棚嫩枝扦插试验简报[J].河北林业科技,1998(1):29.
[29]吕月良,陈璋,施季森,黄宇翔,王加煊.福建山樱花扦插繁殖及其影响因子研究[J].福建林业科技,2006(2):1-6+24.
[30]陆贵巧,林青.樱花嫩枝插扦生根试验[J].经济林研究,1998(3):35.
[31]段晓梅.冬樱花扦插繁殖研究[J].西南林学院学报,2003,23(1):43-45.
[32]张国,赵玉.樱花全光雾插试验初报[J].河北林业科技,1999(4):14-15.
[33]王小蓉,熊庆娥.日本晚樱绿枝扦插生根解剖特性的研究[J].四川农业大学学报,2000(3):249-251.
[34]赵焕志,石岩.樱花蘖芽扦插育苗[J].中国花卉盆景,2001(4):19.
[35]王小蓉,熊庆娥.IBA,树龄,枝段对日本晚樱绿枝扦插生根的影响[J].四川农业大学学报,2000(31):246-248.
[36]田兴旺.樱花嫩枝扦插繁殖技术[J].甘肃农业科技,2002(3):40.
[37]王瑞芳,丛日晨,孙志刚,等.日本晚樱扦插技术研究[J].林业科技通讯,2000(11):26-27.
[38]邹娜,曹光球,林思祖.观赏樱花繁殖技术研究进展[J].西南林学院学报,2007(6):42-46.
[39]周志坚,张慧民.大岛樱的组织培养与繁殖[J].广东林业科技,1994(1):16.
[40]及华.樱花的离体快速繁殖[J].植物生理学通讯,1998(4):269.
[41]王光萍,黄敏仁.福建山樱花的组织培养及植株再生[J].南京林业太学学报,2002(2):73-75.
[42]姚连芳,张建伟,冷天波,周子发.樱花组培快繁生产技术[J].农业科技通讯,2004(2):23.
[43]周丽华,王振师.绯寒樱的组织培养快繁研究[J].广东林业科技,2004(4):1-4.
[44]闫道良,王贤荣,钦佩等.钟花樱组织培养再生体系的建立[J].林业科技开发,2006(3):21-24.
[45]孟月娥,李艳敏,赵秀山,王慧娟.日本晚樱组培快繁技术研究[J].中国农学通报,2006(10):264-266.
[46]王铖,朱红霞.福建山樱花[J].园林;2006(4):39.
[47]陈璋.福建山樱花野生群落物种数量特征与区系分布研究[J].福建林业科技,2007(3):48-52.
[48]苏倩.福建山樱花种源遗传多样性研究[D].南京林业大学,2007:5.
[49]陈璋,吕月良.福建山樱花及其近缘物种研究现状与开发利用[J].福建热作科技,2007(1):32-35.
[50]康木水.福建山樱花种子发育观察与育苗技术研究[J].福建林业科技,2007(3):19-22.
[51]吕月良.福建山樱花群体遗传多样性、繁育技术体系和育种策略研究[D].南京林业大学,2006:6.
[52]吕月良,陈璋,施季森,黄字翔,刘金燕,谢建丽.福建山樱花不定芽诱导和植株再生规模化繁殖试验[J].南京林业大学学报(自然科学版),2006(3):105-108.
[53]陈健妙.我国植物组织培养的现状与前景[J].海南大学学报(自然科学版),2002(4):332-35.
[54]黄宇翔,刘金燕,卓小丽,王肇剑.福建山樱花组培快繁研究[J].中国农学通报,2006(8):162-164.
[55]吕月良,陈樟,施季森.福建山樱花研究现状、开发前景与育种策略[J].南京林业大学学报(自然科学版),2006(1):115-118.
[56]Zenk M H,E1—Shagi H,Schulte U.Anthraquinone production by cell suspension cultures of Morinda citrifolia.Planta Med Suppl,1975,p:97.
[57]李永欣,王义强.植物组织培养的应用研究概述[J].江苏林业科技,2005(3):44-46.
[58]刘欣,薛金芝,赵丹,裴崎君.植物组织培养技木在林木育种中的应用[J].吉林林业科技,2007(3):8-10+13.
[59]胡彦,赵艳.植物组织培养技术的应用以及在培养过程中存在的问题[J].陕西师范大学学报(自然科学版),2004(S1):130-134.
[60]于占江,安殿军,游建刚,宋增柱,李淑玲,邹威.引进日本樱花优良品种栽培及繁育的可行性[J].中国林副特产,2002(1):51.
[61]高崇辉.樱花引种栽培试验[J].青海大学学报(自然科学版),2005(2):47-49.
[62]郭万里.规模化生产樱花试管苗和林木工厂化微繁中的部分问题及对策[D].中国人民解放军军需大学,2003:5.
[63]王慧娟,孟月娥,赵秀山,李艳敏.樱花组培苗的移栽技术研究[J].河南农业科学,2006(11):99-101.
[2]黄雯.中国古代花卉文献研究[D].西北农林科技大学,2003:9.
[3]尚礼.阳春樱花正烂漫[J].中国花卉园艺,2003(7):46.
[4]王铖,朱红霞,周汉其.我国冬季开花植物资源研究进展[J].河北林业科技,2007(3):29-32.
[5]朱红霞,王铖.我国冬季开花植物资源及园林景观营造研究[J].山东林业科技,2006(6):71-74.
[6]陈玲.家门前的樱花树[J].中国花卉园艺,2002(7):45.
[7]冯怀字.樱花——日本人心灵的故乡[J].少年科技博览,2003(Z2):8-9.
[8]王相飞.中日“樱花”意象比较研究[J].南京师范大学文学院学报,2007(2):112-118.
[9]朱荣根,赵瑞云.日本人与樱花[J].知识就是力量,2001(4):32.
[10]闵崇博[日].绮丽的樱花[J].中国园林,2004(6):66-67.
[11]张晋岩[日].留住那场樱花雨——日本樱花面面观[J].中国花卉盆景,2003(5):25-26.
[12]Byun-K- O,Park -H- S,Lee- S-K.Eeffect of position of explants on rooting rate and rot development of Prunus serrulata.Research Report of the Forest Genetics Research Institute,1995(31):106-111.
[13]Baranova M.Principles of comparative stomatographic studies of flowering plants.Botanical Review,1992.58(1):49-92.
[14]老实人.武汉浪漫樱花节——武大赏樱[J].粤港澳市场与价格,2006(4):46-47.
[15]王贤荣,黄国富.中国樱花类植物资源及其开发利用[J].林业科技开发,2001(6):3-5.
[16]段晓梅,樱花繁殖综述[J].思茅师范高等专科学校学报,2002,18(3):82-85.
[17]王艳华,高述民,李凤兰等.大山樱种子休眠机理的探讨[J].种子,2005,24(5):12-16.
[18]Ching-Te Chien,Shun-Ying Chen,Jeng-Chuan Yang.Effect of Stratification and Drying on the Germination and Storage of Prunus campanulata Seeds[J].Taiwan Journal of Forest Science,2002,17(4):413-420.
[19]Shun-Ying Chen,Ching-Te Chien,Jeng-Der Chung etc.Dormancy-break and germination in seeds of Prunus campanulata(Rosaceae):role of covering layers and changes in concentration of abscisic acid and gibberellins[J].Seed Science Research,2007(17):21-32.
[20]王贤荣,黄国富.中国樱花类植物资源及其开发利用[J].林业科技开发,2001,15(6):3-6.
[21]张义,王剑峰.赤霉素浸泡与层积时间对山樱种子萌发的影响[J].长江大学学报(自然科学版),2005,2(8):26-27.
[22]方妙辉.钟花樱桃种子冷处理及生根剂浸种试验[J].福建果树,2006(3):52-53.
[23]徐兆波,郭绍霞.垂枝樱花引种观察与繁育技术研究[J].莱阳农学院学报,2001(1):32-36.
[24]汪雪文.嫁接培育日本樱花大苗技术[J].安徽林业科技,2006(3):29-30.
[25]孙业文.樱花嫁接育苗技术[J].江苏林业科技,2002,29(3):43.
[26]张艳芳.樱花的切接繁殖[J].花木盆景:花卉园艺,2006(2):14-15.
[27]王永清,汤浩茹.几种木本园艺植物塑料袋内密封扦插试验[J].四川农业大学,1997,15(4):486-490.
[28]陆贵巧,林青.樱花荫棚嫩枝扦插试验简报[J].河北林业科技,1998(1):29.
[29]吕月良,陈璋,施季森,黄宇翔,王加煊.福建山樱花扦插繁殖及其影响因子研究[J].福建林业科技,2006(2):1-6+24.
[30]陆贵巧,林青.樱花嫩枝插扦生根试验[J].经济林研究,1998(3):35.
[31]段晓梅.冬樱花扦插繁殖研究[J].西南林学院学报,2003,23(1):43-45.
[32]张国,赵玉.樱花全光雾插试验初报[J].河北林业科技,1999(4):14-15.
[33]王小蓉,熊庆娥.日本晚樱绿枝扦插生根解剖特性的研究[J].四川农业大学学报,2000(3):249-251.
[34]赵焕志,石岩.樱花蘖芽扦插育苗[J].中国花卉盆景,2001(4):19.
[35]王小蓉,熊庆娥.IBA,树龄,枝段对日本晚樱绿枝扦插生根的影响[J].四川农业大学学报,2000(31):246-248.
[36]田兴旺.樱花嫩枝扦插繁殖技术[J].甘肃农业科技,2002(3):40.
[37]王瑞芳,丛日晨,孙志刚,等.日本晚樱扦插技术研究[J].林业科技通讯,2000(11):26-27.
[38]邹娜,曹光球,林思祖.观赏樱花繁殖技术研究进展[J].西南林学院学报,2007(6):42-46.
[39]周志坚,张慧民.大岛樱的组织培养与繁殖[J].广东林业科技,1994(1):16.
[40]及华.樱花的离体快速繁殖[J].植物生理学通讯,1998(4):269.
[41]王光萍,黄敏仁.福建山樱花的组织培养及植株再生[J].南京林业太学学报,2002(2):73-75.
[42]姚连芳,张建伟,冷天波,周子发.樱花组培快繁生产技术[J].农业科技通讯,2004(2):23.
[43]周丽华,王振师.绯寒樱的组织培养快繁研究[J].广东林业科技,2004(4):1-4.
[44]闫道良,王贤荣,钦佩等.钟花樱组织培养再生体系的建立[J].林业科技开发,2006(3):21-24.
[45]孟月娥,李艳敏,赵秀山,王慧娟.日本晚樱组培快繁技术研究[J].中国农学通报,2006(10):264-266.
[46]王铖,朱红霞.福建山樱花[J].园林;2006(4):39.
[47]陈璋.福建山樱花野生群落物种数量特征与区系分布研究[J].福建林业科技,2007(3):48-52.
[48]苏倩.福建山樱花种源遗传多样性研究[D].南京林业大学,2007:5.
[49]陈璋,吕月良.福建山樱花及其近缘物种研究现状与开发利用[J].福建热作科技,2007(1):32-35.
[50]康木水.福建山樱花种子发育观察与育苗技术研究[J].福建林业科技,2007(3):19-22.
[51]吕月良.福建山樱花群体遗传多样性、繁育技术体系和育种策略研究[D].南京林业大学,2006:6.
[52]吕月良,陈璋,施季森,黄字翔,刘金燕,谢建丽.福建山樱花不定芽诱导和植株再生规模化繁殖试验[J].南京林业大学学报(自然科学版),2006(3):105-108.
[53]陈健妙.我国植物组织培养的现状与前景[J].海南大学学报(自然科学版),2002(4):332-35.
[54]黄宇翔,刘金燕,卓小丽,王肇剑.福建山樱花组培快繁研究[J].中国农学通报,2006(8):162-164.
[55]吕月良,陈樟,施季森.福建山樱花研究现状、开发前景与育种策略[J].南京林业大学学报(自然科学版),2006(1):115-118.
[56]Zenk M H,E1—Shagi H,Schulte U.Anthraquinone production by cell suspension cultures of Morinda citrifolia.Planta Med Suppl,1975,p:97.
[57]李永欣,王义强.植物组织培养的应用研究概述[J].江苏林业科技,2005(3):44-46.
[58]刘欣,薛金芝,赵丹,裴崎君.植物组织培养技木在林木育种中的应用[J].吉林林业科技,2007(3):8-10+13.
[59]胡彦,赵艳.植物组织培养技术的应用以及在培养过程中存在的问题[J].陕西师范大学学报(自然科学版),2004(S1):130-134.
[60]于占江,安殿军,游建刚,宋增柱,李淑玲,邹威.引进日本樱花优良品种栽培及繁育的可行性[J].中国林副特产,2002(1):51.
[61]高崇辉.樱花引种栽培试验[J].青海大学学报(自然科学版),2005(2):47-49.
[62]郭万里.规模化生产樱花试管苗和林木工厂化微繁中的部分问题及对策[D].中国人民解放军军需大学,2003:5.
[63]王慧娟,孟月娥,赵秀山,李艳敏.樱花组培苗的移栽技术研究[J].河南农业科学,2006(11):99-101.