膀胱移行细胞癌中Tenascin-C表达的研究
摘要
目的：初步探讨膀胱移行细胞癌(BTCC)中Tenascin-C蛋白m RNA的表达，明确Tenascin-C在肿瘤及瘤旁组织中的表达情况，并对Tenascin-C在BTCC中早期浸润、术后随访及复发的检测等方面的价值作进一步的讨论。方法：三组标本共50例，分别来源于肿瘤组织、肿瘤旁组织、正常膀胱组织。采集标本于膀胱开放手术、膀胱镜活检术或经尿道膀胱肿瘤切除术(TURBT)。应用逆转录聚合酶链反应(RT-PCR)法检测所取标本，观测Tenascin-C蛋白mRNA水平在膀胱不同组织中的表达情况，对Tenascin-C的表达作半定量测算，并与传统的膀胱肿瘤的病理学分级作相关的统计学分析。结果：膀胱移行细胞癌及癌旁组织Tenascin-C的表达显著上调(P＞0.05)，两者没有统计学上的差异，而正常的膀胱粘膜几乎是不表达或仅有少量表达。TN-C的表达与膀胱癌的病理分级(Ⅰ、Ⅱ、Ⅲ)密切相关(r=0.848，p＝0.00＜0.01)。随着级别的增高，TN—C的表达显著上调。结论：在膀胱移行细胞癌早期诊断中，Tenascin-C是一个极有临床意义的指标，尤其是随着经尿道膀胱肿瘤切除(TURBT)术的广泛使用，Tenascin-C在该肿瘤复发的早期诊断中非常敏感，一定程度上反映细胞分化，帮助我们判断预后，同时也是选择最佳的治疗方案的一个重要参数。
Objective To investigate TN-C expression in BTCC, especially its expression in the tumor tissue and paratumor tissue. To evaluate TN-C in the early stage and recurrence of BTCC.and flow-up of postoperation .Methods Three groups of samples were used in this study, which were obtained from tumor tissue, paratumor tissue and normal bladder tissue respectively. The samples were harvested during three operations: the open operation of bladder,biopsy and TURBT. The fifty samples were tested with RT-PCR technique to observe the expression of TN-C protein in different tissue of bladder. TN-C's expression was tested with half-volume technique and associated statistical analysis was done between this expression and traditional pathological levels of bladder tumor. Results The expression of TN-C in tumor tissue and paratumor tissue of BTCC was significantly up regulated, there was no significant different between the two groups, while there was no expression or very little expression in normal bladder tissues. The expr
ession of TN-C has closely correlation with traditional degree of tumor cell differentiation of BTCC( I, II ,III).the higher grade was, the more TN-C expression was. Conclusions: As a very significant clinical value in early diagnosis of BTCC, Tenascin-C also plays an help important role in early diagnosis of BTCC recurrence, It is helpful to estimate the prognosis of patients and choice of right treatment plan. This role becomes even much more significant considering that TURBT technique is extensively applied clinically. TN-C is a sensitive value in early diagnosis of BTCC and is closely associated with the tumor cell differential grades. With this character, TN-C could be useful to evaluate prognosis and choice of treatment methods.
Objective To investigate TN-C expression in BTCC, especially its expression in the tumor tissue and paratumor tissue. To evaluate TN-C in the early stage and recurrence of BTCC.and flow-up of postoperation .Methods Three groups of samples were used in this study, which were obtained from tumor tissue, paratumor tissue and normal bladder tissue respectively. The samples were harvested during three operations: the open operation of bladder,biopsy and TURBT. The fifty samples were tested with RT-PCR technique to observe the expression of TN-C protein in different tissue of bladder. TN-C's expression was tested with half-volume technique and associated statistical analysis was done between this expression and traditional pathological levels of bladder tumor. Results The expression of TN-C in tumor tissue and paratumor tissue of BTCC was significantly up regulated, there was no significant different between the two groups, while there was no expression or very little expression in normal bladder tissues. The expr
ession of TN-C has closely correlation with traditional degree of tumor cell differentiation of BTCC( I, II ,III).the higher grade was, the more TN-C expression was. Conclusions: As a very significant clinical value in early diagnosis of BTCC, Tenascin-C also plays an help important role in early diagnosis of BTCC recurrence, It is helpful to estimate the prognosis of patients and choice of right treatment plan. This role becomes even much more significant considering that TURBT technique is extensively applied clinically. TN-C is a sensitive value in early diagnosis of BTCC and is closely associated with the tumor cell differential grades. With this character, TN-C could be useful to evaluate prognosis and choice of treatment methods.
引文
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[2] Scheetz J F, Lloyd J P. the tenascin family of ECM glycoproteins structure, function, and regulation during embryonic development and tissue remodeling. Developmental Dynamics,2000,218;235-242.
[3] Booth c, handen pet al, towords defining roles and relationship for tenascin-c and tgfbeta-1 in the normal and neoplasmic urinary bladder j pathol 2002 nov:198(3):359-68
[4] Matteu Adams,J.Louise Jones,et al Changes in tenascin-C Isoform expression in invasive and preinvasive Breast Disease. Eur.J. Cancer Research, 2002 62;3289-3297,
[5] 黄文晋，肖华胜，张萍等。大鼠大脑皮层cDNA文库的构建。第四军医大学学报，2000，21(1)：127—128。
[6] Jones FS,Jones PL. The tenascin family of ECM glycoproyein structure, function, and regulation during embryonic development and tissue remodeling[J]. Dev Dyn,2000,218(2):235—242.
[7] Clark RA, Erickson HP, Springer TA. Tenascin supports lymphocyte rolling. J cell Boil, 1997,137:755-765
[8] TruongLD,Pindur J, Fostor S, et al.Tenascin expression in nephrogenesis and in normal or pathologyic glomerulus morphologyic features and functional implications [J]. Nephron,1996,72:499-506.
[9] Belanger I, Beaulieu JF.Tenascin in the developing and adult human intestine[J]. Histol Histopathol,2000,15(2):577～585.
[10].Chiquet-Ehrismann R, Mackie EJ, Pearson CA.et al.tenascin:an extracellular matrix protein involved in tissue interactions during fetal development and on cogcnesiss, cell. 1986, 47:131.
[11] Meiners Smarone M,Rittenhous JL, et al. Regulation of astrocytic tenascin by basic fibroblast growth factor[J]. Deval Biol, 1993,160(2): 480.
[12] Tucker RP, Hammarback JA,Jenrath DA ,et al.Tenascin expression in the mouse;in situ localization and induction in vitro by b FGF [J],J CELL Sci,1993,104;69-76
[13] .Ekblam M, Fassler R, Tomasini JB, et al. Downregulation of tenascin expression in bone marrow syromal cells and in fibroblasts[J]. J Cell Biol 1993,123;1037-1045.
[14] Chiquet-Ehrismann R.Tenascin,a growing family of extracelluar matrixproteins. Experiment, 1995;51(9-10):853.
[15] Lightner VA .tenascin:does it play a role in epidermal morphogenesis and homeostasis? [J].J lnzest Dematol 1994,102(3):273-277.
[16] Chung C Y, Murphy-Ullrich J E.Erickson H P. Mitogenesis. Cell migration. And loss of adhesionsinduced by tenascin-c interacting with its cell surface receporannexin-2Mmol Boil Cell, 1996,7:883 -892.
[17]. Goussia AC Ioachinm E E. Peschos D.Eexpression of the extracellular matrix protein tenascin in the layngeal epithelial lesion:Correlation with fibronection,CD44, cathepsind and proliferation indices.Virchow Arch,2000,436:579-584.
[18] Lacour JP, Vitetta A,Chiquet-Ehrismann R,et al.Tenascin in the dermis in cleroderma. Br J Dermatol,1992,127:328.
[19] 丁振若 苏明权，临床RCR基因诊断技术，世界图书出版社，1998，9；2—8
[20].Grocela J A, Mc Dougal, W S. Utility of nuclear matrix protein(nmp22) in the detecting bladder cancer [J] Urol Clin North AM 2000, 27(1):47-51
[21].Sela, M,C,B.Anfinsen,W.F. Harrington. The correlation of ribonuclease activate with special aspects of tertiary structure. Biochem.biophys.Acta.1957;26:502
[22] Chirgwin,J.M.,A.E.Przybyla, R.J.Macdonald,et al. Isolation of biologically activate ribonucleic acid from sources enriched in ribonuclease.Biochemistry.1979;18:5294
[23] Chomczynski P, Sacchi N.Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem.1987;162:156-191
[2] Scheetz J F, Lloyd J P. the tenascin family of ECM glycoproteins structure, function, and regulation during embryonic development and tissue remodeling. Developmental Dynamics,2000,218;235-242.
[3] Booth c, handen pet al, towords defining roles and relationship for tenascin-c and tgfbeta-1 in the normal and neoplasmic urinary bladder j pathol 2002 nov:198(3):359-68
[4] Matteu Adams,J.Louise Jones,et al Changes in tenascin-C Isoform expression in invasive and preinvasive Breast Disease. Eur.J. Cancer Research, 2002 62;3289-3297,
[5] 黄文晋，肖华胜，张萍等。大鼠大脑皮层cDNA文库的构建。第四军医大学学报，2000，21(1)：127—128。
[6] Jones FS,Jones PL. The tenascin family of ECM glycoproyein structure, function, and regulation during embryonic development and tissue remodeling[J]. Dev Dyn,2000,218(2):235—242.
[7] Clark RA, Erickson HP, Springer TA. Tenascin supports lymphocyte rolling. J cell Boil, 1997,137:755-765
[8] TruongLD,Pindur J, Fostor S, et al.Tenascin expression in nephrogenesis and in normal or pathologyic glomerulus morphologyic features and functional implications [J]. Nephron,1996,72:499-506.
[9] Belanger I, Beaulieu JF.Tenascin in the developing and adult human intestine[J]. Histol Histopathol,2000,15(2):577～585.
[10].Chiquet-Ehrismann R, Mackie EJ, Pearson CA.et al.tenascin:an extracellular matrix protein involved in tissue interactions during fetal development and on cogcnesiss, cell. 1986, 47:131.
[11] Meiners Smarone M,Rittenhous JL, et al. Regulation of astrocytic tenascin by basic fibroblast growth factor[J]. Deval Biol, 1993,160(2): 480.
[12] Tucker RP, Hammarback JA,Jenrath DA ,et al.Tenascin expression in the mouse;in situ localization and induction in vitro by b FGF [J],J CELL Sci,1993,104;69-76
[13] .Ekblam M, Fassler R, Tomasini JB, et al. Downregulation of tenascin expression in bone marrow syromal cells and in fibroblasts[J]. J Cell Biol 1993,123;1037-1045.
[14] Chiquet-Ehrismann R.Tenascin,a growing family of extracelluar matrixproteins. Experiment, 1995;51(9-10):853.
[15] Lightner VA .tenascin:does it play a role in epidermal morphogenesis and homeostasis? [J].J lnzest Dematol 1994,102(3):273-277.
[16] Chung C Y, Murphy-Ullrich J E.Erickson H P. Mitogenesis. Cell migration. And loss of adhesionsinduced by tenascin-c interacting with its cell surface receporannexin-2Mmol Boil Cell, 1996,7:883 -892.
[17]. Goussia AC Ioachinm E E. Peschos D.Eexpression of the extracellular matrix protein tenascin in the layngeal epithelial lesion:Correlation with fibronection,CD44, cathepsind and proliferation indices.Virchow Arch,2000,436:579-584.
[18] Lacour JP, Vitetta A,Chiquet-Ehrismann R,et al.Tenascin in the dermis in cleroderma. Br J Dermatol,1992,127:328.
[19] 丁振若 苏明权，临床RCR基因诊断技术，世界图书出版社，1998，9；2—8
[20].Grocela J A, Mc Dougal, W S. Utility of nuclear matrix protein(nmp22) in the detecting bladder cancer [J] Urol Clin North AM 2000, 27(1):47-51
[21].Sela, M,C,B.Anfinsen,W.F. Harrington. The correlation of ribonuclease activate with special aspects of tertiary structure. Biochem.biophys.Acta.1957;26:502
[22] Chirgwin,J.M.,A.E.Przybyla, R.J.Macdonald,et al. Isolation of biologically activate ribonucleic acid from sources enriched in ribonuclease.Biochemistry.1979;18:5294
[23] Chomczynski P, Sacchi N.Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem.1987;162:156-191