制草乌白芍组分群配伍抗炎镇痛作用的实验研究
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摘要
目的:以制草乌白芍配伍水煎液的镇痛、抗炎作用为基础,进一步探讨制草乌白芍组分群配伍的镇痛、抗炎及解毒作用。
方法:从水煎液和组分群配伍两个层面研究制草乌配伍白芍的镇痛、抗炎作用。在水煎液层面,选用制草乌白芍1:1、1:2、2:1、2:3配伍水煎液,采用热板致痛试验、乙酸扭体致痛试验、二甲苯耳廓肿胀试验及痹证模型,研究制草乌白芍不同配伍比例水煎液的镇痛、抗炎作用;在组分群层面,采用均匀设计方法进行组分群配伍比例的设计,运用热板致痛试验、乙酸扭体致痛试验、二甲苯耳廓肿胀试验、蛋清足肿胀试验,研究制草乌白芍组分群不同配伍比例的镇痛、抗炎作用,并优化其配伍比例。采用急性毒性实验研究其组分群配伍的毒性及解毒作用。
结果:制草乌配伍白芍水煎液能升高小鼠热板疼痛模型、小鼠乙酸扭体疼痛模型、和痹证大鼠足部的痛阈值,抑制小鼠二甲苯耳廓肿胀和痹证大鼠足部肿胀。制草乌白芍组分群配伍能升高小鼠热板疼痛模型、小鼠乙酸扭体疼痛模型的痛阈值,抑制小鼠二甲苯耳廓肿胀和大鼠蛋清足部肿胀。随着制草乌总碱量的增加,制草乌白芍组分群配伍的LD_(50)明显减小。
结论:制草乌配伍白芍水煎液有显著的镇痛、抗炎作用,其中制草乌白芍1:2镇痛效果较好,制草乌白芍2:3抗炎效果较好。制草乌白芍组分群配伍有显著的抗炎镇痛作用;其配伍比例的改变可导致组分群起效时间不同;且在不同的时间段,起镇痛、抗炎作用的主要组分不同,配伍比例也不同。总结不同时间段组分群配伍比例的优化结果推测出:在镇痛作用中,制草乌总碱发挥镇痛作用强,起效快,白芍总苷、白芍多糖的镇痛作用起效较慢;在抗炎作用中,制草乌水碱、白芍总苷是起抗炎作用的主要组分。从上述结果我们还可以推测出制草乌总碱、白芍总苷、白芍多糖的代谢可能存在双峰现象。此外,制草乌白芍组分群配伍的毒性随着制草乌总碱配伍比例的增加而增加,且有一定的解毒作用。
Objective:Based the effect of anti-inflammation and analgesia about the water decoction about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba,the aim of this article is to approach the compatibility of composition group about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba on anti-inflammation effect, analgesia effect and deintoxication.
Methods:It is to research research the compatibility effect of the water decoction and composition-group about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba on anti-inflammation effect,analgesia effect and deintoxication.On the water decoction deck,it is adoptted the water decoction of Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba 1:1、1:2、2:1、2:3,and,the pain test induced by hot-plate,the body-twisting test induced by acetic acid,the swelling test of the auricles induced by xylene,and cold and dampness syndrome rheumatoid arthritis model are used to research the different compatibility proportion effect of the water decoction about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba on anti-inflammation and analgesia.On the composition-group deck,the uniformdesign method is used to design the compatibility proportion of the composition-group,the pain test induced by hot-plate,the body-twisting test induced by acetic acid,the swelling test of the auricles induced by xylene,and the swelling degree test of feet induced by egg white are used to research the different compatibility proportion effect of the composition-group about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba on anti-inflammation and analgesia. The acute toxicity testing is used to research the toxicity and deintoxication of the composition-group compatibility about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba.
Results:The compatibility water decoction about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba could elevate the pain threshold of mice induced by hot-plate and acetic acid,and restrain the swelling of the mouse auricles induced by xylene and the volume of Bi-Zheng rats's feet.The composition-group compatibility about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba could elevate the pain threshold of mouse induced by hot-plate and acetic acid,and restrain the swelling of the mouse auricles induced by xylene and the swelling of rat feet induced by egg white.Following the dosage of Radix Aconiti Kusnezoffi preparata total base increased,LD_(50) of the composition-group compatibility about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba has diminished tendency obviously.
Conclusions:the water decoction of Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba have obviously effect on the analgesia and anti-inflammatory,among them different proportion,the better ratios of analgesia and anti-inflammatory are 1:2 and 2:3 respectively.the compatibility of composition-group about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba have the effect of analgesia and antiinflammatory,key components playing a part in analgesia and antiinflammatory are different,and compatibility-proportion are different,the react times are different also.The following conclusions can be supposed from the optimized result of compatibility proportion in different times:Radix Aconiti Kusnezoffi preparata total base has strong analgesic effect,and react fast.The analgesic effect on total glucosides and polycose of Raidix Paeonia e Alba react slowly.on the anti-inflammatory effect,Maybe there is a double hump manifest on the the metabolism of Radix Aconiti Kusnezoffi preparata water-solubility alkaloid、total glucosides and polycose of Raidix Paeoniae Alba.Besides,following the dosage of Radix Aconiti Kusnezoffi preparata total base increased,the toxicity of the compatibility of composition-group about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba is increased.But a few proportion of them have some deintoxication.
方法:从水煎液和组分群配伍两个层面研究制草乌配伍白芍的镇痛、抗炎作用。在水煎液层面,选用制草乌白芍1:1、1:2、2:1、2:3配伍水煎液,采用热板致痛试验、乙酸扭体致痛试验、二甲苯耳廓肿胀试验及痹证模型,研究制草乌白芍不同配伍比例水煎液的镇痛、抗炎作用;在组分群层面,采用均匀设计方法进行组分群配伍比例的设计,运用热板致痛试验、乙酸扭体致痛试验、二甲苯耳廓肿胀试验、蛋清足肿胀试验,研究制草乌白芍组分群不同配伍比例的镇痛、抗炎作用,并优化其配伍比例。采用急性毒性实验研究其组分群配伍的毒性及解毒作用。
结果:制草乌配伍白芍水煎液能升高小鼠热板疼痛模型、小鼠乙酸扭体疼痛模型、和痹证大鼠足部的痛阈值,抑制小鼠二甲苯耳廓肿胀和痹证大鼠足部肿胀。制草乌白芍组分群配伍能升高小鼠热板疼痛模型、小鼠乙酸扭体疼痛模型的痛阈值,抑制小鼠二甲苯耳廓肿胀和大鼠蛋清足部肿胀。随着制草乌总碱量的增加,制草乌白芍组分群配伍的LD_(50)明显减小。
结论:制草乌配伍白芍水煎液有显著的镇痛、抗炎作用,其中制草乌白芍1:2镇痛效果较好,制草乌白芍2:3抗炎效果较好。制草乌白芍组分群配伍有显著的抗炎镇痛作用;其配伍比例的改变可导致组分群起效时间不同;且在不同的时间段,起镇痛、抗炎作用的主要组分不同,配伍比例也不同。总结不同时间段组分群配伍比例的优化结果推测出:在镇痛作用中,制草乌总碱发挥镇痛作用强,起效快,白芍总苷、白芍多糖的镇痛作用起效较慢;在抗炎作用中,制草乌水碱、白芍总苷是起抗炎作用的主要组分。从上述结果我们还可以推测出制草乌总碱、白芍总苷、白芍多糖的代谢可能存在双峰现象。此外,制草乌白芍组分群配伍的毒性随着制草乌总碱配伍比例的增加而增加,且有一定的解毒作用。
Objective:Based the effect of anti-inflammation and analgesia about the water decoction about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba,the aim of this article is to approach the compatibility of composition group about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba on anti-inflammation effect, analgesia effect and deintoxication.
Methods:It is to research research the compatibility effect of the water decoction and composition-group about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba on anti-inflammation effect,analgesia effect and deintoxication.On the water decoction deck,it is adoptted the water decoction of Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba 1:1、1:2、2:1、2:3,and,the pain test induced by hot-plate,the body-twisting test induced by acetic acid,the swelling test of the auricles induced by xylene,and cold and dampness syndrome rheumatoid arthritis model are used to research the different compatibility proportion effect of the water decoction about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba on anti-inflammation and analgesia.On the composition-group deck,the uniformdesign method is used to design the compatibility proportion of the composition-group,the pain test induced by hot-plate,the body-twisting test induced by acetic acid,the swelling test of the auricles induced by xylene,and the swelling degree test of feet induced by egg white are used to research the different compatibility proportion effect of the composition-group about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba on anti-inflammation and analgesia. The acute toxicity testing is used to research the toxicity and deintoxication of the composition-group compatibility about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba.
Results:The compatibility water decoction about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba could elevate the pain threshold of mice induced by hot-plate and acetic acid,and restrain the swelling of the mouse auricles induced by xylene and the volume of Bi-Zheng rats's feet.The composition-group compatibility about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba could elevate the pain threshold of mouse induced by hot-plate and acetic acid,and restrain the swelling of the mouse auricles induced by xylene and the swelling of rat feet induced by egg white.Following the dosage of Radix Aconiti Kusnezoffi preparata total base increased,LD_(50) of the composition-group compatibility about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba has diminished tendency obviously.
Conclusions:the water decoction of Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba have obviously effect on the analgesia and anti-inflammatory,among them different proportion,the better ratios of analgesia and anti-inflammatory are 1:2 and 2:3 respectively.the compatibility of composition-group about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba have the effect of analgesia and antiinflammatory,key components playing a part in analgesia and antiinflammatory are different,and compatibility-proportion are different,the react times are different also.The following conclusions can be supposed from the optimized result of compatibility proportion in different times:Radix Aconiti Kusnezoffi preparata total base has strong analgesic effect,and react fast.The analgesic effect on total glucosides and polycose of Raidix Paeonia e Alba react slowly.on the anti-inflammatory effect,Maybe there is a double hump manifest on the the metabolism of Radix Aconiti Kusnezoffi preparata water-solubility alkaloid、total glucosides and polycose of Raidix Paeoniae Alba.Besides,following the dosage of Radix Aconiti Kusnezoffi preparata total base increased,the toxicity of the compatibility of composition-group about Radix Aconiti Kusnezoffi preparata and Raidix Paeoniae Alba is increased.But a few proportion of them have some deintoxication.
引文
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