磁场对成纤维细胞的影响及量效关系研究
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摘要
近年来随着非药物疗法与保健的兴起,磁疗因具有操作简单、无创无痛的特点,而越来越受到人们的关注,对磁场生物效应的报道也越来越多。
但是目前磁疗基础研究薄弱,临床上无严格使用规范,磁对生物体作用的量效关系和安全性研究尚不够深入,磁对生物体作用的内在机制不清楚。本研究在对磁疗用永磁磁源空间磁场分析的基础上,对正常培养和缺氧培养条件下的成纤维细胞受磁场影响的结果进行研究,分析了磁场对成纤维细胞作用的量效关系,探讨了磁场对生物体的作用机制。本文的主要研究内容包括:
1.分析磁疗用圆片永磁磁源的空间磁感强度分布,设计体外细胞培养加磁方法,准确掌握磁场作用于细胞的磁感强度。
2.通过MTT法检测细胞的活性,研究磁场对成纤维细胞增殖活性的影响。实验发现,选取的不同磁感强度磁场均不同程度上提高了成纤维细胞的活性。
3.通过测细胞培养液上清中LDH的含量,研究磁场对成纤维细胞膜损伤的影响。结果发现,实验中选取的大部分磁源对成纤维细胞膜是安全的。
4.通过测量细胞培养液上清中SOD与MDA的含量,研究了不同强度永磁磁场对细胞氧化损伤的影响。结果显示为,细胞的抗氧化性经过磁场的作用后被加强了。但氧化损伤的产物有多有少。
5.利用流式细胞仪检测磁场对成纤维细胞凋亡的影响,从实验结果来看,不同强度永磁磁场对成纤维细胞凋亡的影响显现出了磁场对细胞的阈值作用。但大多磁场组对于成纤维细胞凋亡的影响并无规律可循。
本文研究不同磁感强度永磁磁源对成纤维细胞的影响,从细胞水平探讨磁场的定量作用效果和量效关系,为规范永磁磁源的使用和磁作用效果的研究提供基础性实验依据。明确提出磁场的有效作用磁感强度量值或磁感强度量值范围、以及磁场安全使用的磁感强度量值。
With the recent rise in the popularity of noninvasive drugless therapy and the increasing emphasis on healthcare maintenance, magnetic treatments are getting more and more attention because it is simple to use and painless. Its development and biological effects are now often reported in the media.
The research on magnetic therapy is at its infancy and the clinical use is still not strictly regulated. Its dose-effect relationships, effect on the biological internal mechanism and overall safety are unclear. Therefore much theoretical work, systematic research, and in-depth explorations are still necessary. From applying spatial magnetic field from permanent magnet for magnetotherapy, this study analyzed the effect and dose-effect relationship on fibroblast and organic mechanisms under both normal and hypoxic conditions.
An outline of the main research follows:
1. Analyzed the spatial distribution of magnetic induction of the round permanent magnet and designed methods to apply to in vitro cell culture accurately for carefully designed levels of magnetic strength.
2. Measured cell activities by MTT to study the effect of magnetic field on fibroblast proliferation. Results showed that selected magnetic fields with varying magnetic induction strengths all increased the fibroblast activities to different degrees.
3. By measuring LDH in the cell culture medium, studied the effect of magnetic fields on fibroblast cell membrane damage. It was found that most of the selected magnetic fields in the experiment do not damage fibroblast cell membranes.
4. By measuring SOD and MDA in cell culture medium, studied the effect of different magnetic induction on cells from oxidative injury. The result was that cell antioxidant was enhanced, but the oxidative injuries varied.
5. Using flow cytometry detect the effect of magnetic field on fibroblast apoptosis. The results identified the effect from different levels of magnetic field strength and the associated safety thresholds. But the study could not extrapolate a simple rule between the magnetic induction strengths and the apoptosis results.
This paper researched the effect from applying magnetotherapy on fibroblast, measured its effects from well-designed strength levels and amounts, and provided basic experimental evidence from using a standard permanent magnetic field. Effective magnetic strengths, dosages, and associated safety thresholds were accurately documented.
但是目前磁疗基础研究薄弱,临床上无严格使用规范,磁对生物体作用的量效关系和安全性研究尚不够深入,磁对生物体作用的内在机制不清楚。本研究在对磁疗用永磁磁源空间磁场分析的基础上,对正常培养和缺氧培养条件下的成纤维细胞受磁场影响的结果进行研究,分析了磁场对成纤维细胞作用的量效关系,探讨了磁场对生物体的作用机制。本文的主要研究内容包括:
1.分析磁疗用圆片永磁磁源的空间磁感强度分布,设计体外细胞培养加磁方法,准确掌握磁场作用于细胞的磁感强度。
2.通过MTT法检测细胞的活性,研究磁场对成纤维细胞增殖活性的影响。实验发现,选取的不同磁感强度磁场均不同程度上提高了成纤维细胞的活性。
3.通过测细胞培养液上清中LDH的含量,研究磁场对成纤维细胞膜损伤的影响。结果发现,实验中选取的大部分磁源对成纤维细胞膜是安全的。
4.通过测量细胞培养液上清中SOD与MDA的含量,研究了不同强度永磁磁场对细胞氧化损伤的影响。结果显示为,细胞的抗氧化性经过磁场的作用后被加强了。但氧化损伤的产物有多有少。
5.利用流式细胞仪检测磁场对成纤维细胞凋亡的影响,从实验结果来看,不同强度永磁磁场对成纤维细胞凋亡的影响显现出了磁场对细胞的阈值作用。但大多磁场组对于成纤维细胞凋亡的影响并无规律可循。
本文研究不同磁感强度永磁磁源对成纤维细胞的影响,从细胞水平探讨磁场的定量作用效果和量效关系,为规范永磁磁源的使用和磁作用效果的研究提供基础性实验依据。明确提出磁场的有效作用磁感强度量值或磁感强度量值范围、以及磁场安全使用的磁感强度量值。
With the recent rise in the popularity of noninvasive drugless therapy and the increasing emphasis on healthcare maintenance, magnetic treatments are getting more and more attention because it is simple to use and painless. Its development and biological effects are now often reported in the media.
The research on magnetic therapy is at its infancy and the clinical use is still not strictly regulated. Its dose-effect relationships, effect on the biological internal mechanism and overall safety are unclear. Therefore much theoretical work, systematic research, and in-depth explorations are still necessary. From applying spatial magnetic field from permanent magnet for magnetotherapy, this study analyzed the effect and dose-effect relationship on fibroblast and organic mechanisms under both normal and hypoxic conditions.
An outline of the main research follows:
1. Analyzed the spatial distribution of magnetic induction of the round permanent magnet and designed methods to apply to in vitro cell culture accurately for carefully designed levels of magnetic strength.
2. Measured cell activities by MTT to study the effect of magnetic field on fibroblast proliferation. Results showed that selected magnetic fields with varying magnetic induction strengths all increased the fibroblast activities to different degrees.
3. By measuring LDH in the cell culture medium, studied the effect of magnetic fields on fibroblast cell membrane damage. It was found that most of the selected magnetic fields in the experiment do not damage fibroblast cell membranes.
4. By measuring SOD and MDA in cell culture medium, studied the effect of different magnetic induction on cells from oxidative injury. The result was that cell antioxidant was enhanced, but the oxidative injuries varied.
5. Using flow cytometry detect the effect of magnetic field on fibroblast apoptosis. The results identified the effect from different levels of magnetic field strength and the associated safety thresholds. But the study could not extrapolate a simple rule between the magnetic induction strengths and the apoptosis results.
This paper researched the effect from applying magnetotherapy on fibroblast, measured its effects from well-designed strength levels and amounts, and provided basic experimental evidence from using a standard permanent magnetic field. Effective magnetic strengths, dosages, and associated safety thresholds were accurately documented.
引文
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[2]杨连香,李芬芳,孙惠萍,等.康复锻炼联合磁疗用于膝关节周围骨折术后患者的护理[J].护理与康复,2010,9(5):423—424.
[3] Sekino M, Yamaguchi K, Iriguchi N, et al. Conductivity tensor imaging of the brain using diffusion-weighted magnetic resonance imaging [ J]. App l Phys, 2003, 93(10): 6730-6732.
[4]朱杰西.稳恒磁场抑制肿瘤增殖的实验研究与理论探讨[J].生物磁学, 2006, 6(1): 10-13.
[5] Yamaguchi S, Ogiue-Ikeda M, Sekino M, et al.Effects of pulsed magnetic stimulation on tumor development and immune functions in mice[J]. Bioelectromagnetics, 2006, 27(1): 64-72.
[6] Ghibelli L, Cerella C, Cordisco S, et al. NMR exposure sensitizes tumor cells to apoptosis[J]. Apoptosis, 2006, 11(3): 359-365.
[7]杨逢瑜,磁场对肿瘤细胞的抑制作用,生物磁学,2004,1:1-4
[8]曹建平,骞爱荣,张维等,0.2-0.4 T磁场对肿瘤细胞生长和黏附功能的影响,世界华人消化杂志,2010,18(13):1337～1343
[9]齐浩,艾霞,孙润广,等. AFM观察稳恒磁场对细胞表面精细结构的影响[J].电子显微学报,2009,28(2):150.
[10]程婕,稳恒磁场促进乳腺癌MCF-7细胞凋亡及其对阿霉素增敏的实验研究,硕士学位论文,西安,第四军医大学,2009
[11]陈文芳,齐浩,孙润广,稳恒磁场对人白血病细胞K562生长抑制作用研究初报,西安文理学院学报,2005,8(1):11～14
[12]王光源,弱恒磁场对白血病细胞内钙离子浓度的影响,湖南工业职业技术学院学报,2005,5(3):22～23
[13]宋国丽,苏海静,张小云.不同强度的静磁场对K562细胞的作用[J].中国康复医学杂志,2009,24(3):204.
[14]胡丽芳,骞爱荣,杨鹏飞,等.中等强度静磁场对白血病细胞增殖和细胞周期的影响[J].第四军医大学学报,2009,30(5):397.
[15] Strelczyk D,Eichhorn ME, Luedemann S etc,Static magnetic fields impair angiogenesis and growth of solid tumors in vivo , CANCER BIOLOGY & THERAPY,2009,8(18):1756～1762
[16]邹仲之.组织学与胚胎学[M].6版.北京:人民卫生出版社,2004:22.23.
[17] Gohra H, McDonald T, Verrier ED, et al. Endothelial loss and regeneration in a model of transplant atherosclerosis[J].Transplantation, 2005, 60(1):96-102.
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