宣木瓜组织培养及其影响因子的研究
|
摘要
本论文以宣木瓜的种子为材料,进行了组织培养和耐盐性两个方面的实验。
1.组织培养方面的主要结论如下:
1.1不定芽快速繁殖、诱导生根:
与KT和TDZ相比,BA对不定芽增殖具有更好的效果。NAA与IBA两种生长调节物质对不定芽增殖的影响没有明显的差异。木瓜不定芽在BA0.5 mg/l +NAA0.2 mg/l培养基中,增殖系数最高。降低MS大量元素对不定芽增殖具有负作用。在1/2MS+IBA0.2组合中,不定芽的根诱导率较高、根较长。
1.2愈伤组织的诱导和继代培养:
1.2.1愈伤组织的诱导
①在子叶诱导实验中,2,4-D能比NAA更高效的诱导出愈伤组织,愈伤生长量大,而且经过继代培养后仍能保持较好的长势。2,4-D与KT组合的效果好于2,4-D与BA的组合。筛选试验表明:MS+2,4-D1mg/l+KT0.1mg/l对子叶愈伤诱导及其生长效果较好。②胚轴愈伤组织诱导率达到87%,而子叶较低为69%。虽然NAA诱导的效果较好,但愈伤组织生长速度较慢;而2,4-D则能高效地诱导出愈伤,但所诱导的愈伤容易出现玻璃化现象。提高NAA,2,4-D和BA的浓度,愈伤呈现水浸状的比例和程度都会相应的提高。所设计的组合中,2,4-D 0.1mg/l+BA 0.3 mg/l对胚轴愈伤的诱导效果较好。③暗培养能提高愈伤组织的生长量.随着黑暗培养时间的延长,愈伤组织松散程度也不断上升,子叶颜色由浓绿色逐步向淡黄色过渡。
1.2.2愈伤组织的继代培养
①在固体培养基中,子叶愈伤组织继代培养可以根据目的不同选取不同的继代培养基。长期继代培养,将愈伤组织继代培养在NAA1.0mg/l+KT0.5mg/l的培养基或NAA0.5 mg/l与TDZ 2mg/l的组合中,可得到较好的效果。如需短期内获得较多的愈伤组织,可将愈伤组织在2,4-D1.0mg/l+KT0.1mg/l中培养。②子叶愈伤在液体培养条件下,生长量较低,效果较差。③胚轴愈伤在NAA1mg/l+KT 0.3mg/l培养基中的生长量最大。④维生素C对愈伤组织降低褐化的作用较小,但能促进愈伤组织变绿。
1.3不定芽的诱导发生:
子叶诱导不定芽的培养基中需要添加一定量的TDZ和NAA。不定芽有玻璃化现象,在TDZ 0.5 mg/l + NAA 1.0mg/l的培养基中得到的不定芽发育较好。
2.不同NaCl浓度对种子和愈伤组织的生长的影响,主要结论如下:
2.1愈伤组织:当NaCl浓度较低时,愈伤组织的生长量未受到较大影响、MDA含量较低,POD、SOD活性提高,细胞内可溶性糖、脯氨酸含量升高。而在高浓度NaCl的胁迫下,愈伤组织的生长具有明显的抑制作用、MDA含量升高,而POD、SOD活性开始降低、可溶性糖含量也降低,但是脯氨酸含量一直升高。
2.2幼苗
随着NaCl浓度的升高,种子的萌发率和幼苗根系活力呈递减趋势;除了在0.5‰NaCl处理的幼苗中,其叶绿素含量高于对照,其余处理均低于对照;幼苗的SOD、POD活性变化趋势较一致,先升高后降低,在1‰NaCl浓度具有最好的活性。MDA含量随NaCl处理浓度的升高而升高。
Take the seeds of Chaenomeles speciosa Nakai as plant material; we carried out researches about tissue culture and salt tolerant.
1. The most important conclusion about tissue culture, as follows:
1.1 Multiply and root of adventitious:
Compared KT and TDZ, BA has a better effect on multiply adventitious buds of Chaenomeles speciosa Nakai. The effect of NAA and IBA on multiply adventitious buds is similar. The explants cultured on medium supplemented with BA0.5 mg/l +NAA0.2 mg/l have the highest multiply ability. Reducing the content of MS macro element has a negative effect on multiply adventitious buds. The adventitious buds cultured on medium contained 1/2MS+IBA0.2 mg/l have highest induction root rate, and inducted roots is longer than other combination medium.
1.2 Induction and subculture of callus tissue:
1.2.1 Callus induction
①In the experiment of cotyledons, 2,4-D has a better effect on inducing and subculture callus tissue than that of NAA. A satisfactory result of callus tissue induction and callus tissue growth was achieved on MS medium supplemented with 1mg/l 2,4-D and 0.1mg/l KT.②Callus tissue induction rate of hypocotyls was 87%, and it was higher than that of cotyledons which was 69%. The proportion and the degree of callus vitrifaction would scaled up as increasing the concentration of auxin and BA. Best callus of hypocotyls were obtained on MS medium contained 0.1mg/l 2,4-D and 0.3mg/l BA .③Dark culture incentives callus tissue growth. Longer the days of dark culture, the looser of callus tissue. The colors of cotyledons transferred from dark green to light yellow with increasing dark culture.
1.2.2 Subculture of callus tissue
①On solid medium, the subculture of cotyledons has different culture methods. If the subculture with long time, callus tissue was cultured on medium supplemented with NAA1.0mg/l+KT0.5mg/l or NAA0.5 mg/l + TDZ 2mg/l, which can obtain better result. On the other hand, callus tissue can growth quickly on medium contained 2,4-D 0.1 mg/l +BA 0.3mg/l and we can get more callus tissue in a short time.②Cultured in liquid medium, callus tissue of cotyledons grew slowly.③Callus tissue of hypocotyls growth most rapidly when cultured on medium contained NAA1mg/l+KT0.3mg/l.④The effect of Vc on reducing browning of callus tissue of Chaenomeles speciosa Nakai was less, but promoted colors of callus tissue turn to green.
1.3 Induction adventitious buds
Induction adventitious buds from cotyledons, it was essential that added some TDZ and NAA into induction medium. Some adventitious buds were vitrifaction, and the adventitious buds were growth normally when induced from the medium
supplemented with TDZ 0.5 mg/l + NAA 1.0mg/l.
2. The effect of different concentration NaCl on the growth of seeds and callus tissue, the main conclusion as follows:
2.1 Callus tissue
In low concentration of NaCl, the growth of callus tissue kept normally level、the content of MDA was lower than check, and the activity of POD and SOD higher than check, the content of soluble sugar and Pro was higher than check. When cultured on a high NaCl concentration, the growth of callus tissue reduced rapidly, the content of MDA was increased, and the activity of POD and SOD reduced; the content of soluble sugar also reduced, but the content of Pro was still increased.
2.2 Seedings
With the increasing of NaCl concentration, the germination of seeds and activity of root system was reduced; except the seeds growth in 0.5‰NaCl, the content of seeds leave’s chlorophyll in the others treatment was lower than blank. Firstly the activity of plantlets’SOD and POD increased, subsequently decreased, and highest enzyme activity was obtain when the seeds cultured on medium contained 1‰NaCl. The content of MDA was scaled up with the increasing of NaCl concentration.
1.组织培养方面的主要结论如下:
1.1不定芽快速繁殖、诱导生根:
与KT和TDZ相比,BA对不定芽增殖具有更好的效果。NAA与IBA两种生长调节物质对不定芽增殖的影响没有明显的差异。木瓜不定芽在BA0.5 mg/l +NAA0.2 mg/l培养基中,增殖系数最高。降低MS大量元素对不定芽增殖具有负作用。在1/2MS+IBA0.2组合中,不定芽的根诱导率较高、根较长。
1.2愈伤组织的诱导和继代培养:
1.2.1愈伤组织的诱导
①在子叶诱导实验中,2,4-D能比NAA更高效的诱导出愈伤组织,愈伤生长量大,而且经过继代培养后仍能保持较好的长势。2,4-D与KT组合的效果好于2,4-D与BA的组合。筛选试验表明:MS+2,4-D1mg/l+KT0.1mg/l对子叶愈伤诱导及其生长效果较好。②胚轴愈伤组织诱导率达到87%,而子叶较低为69%。虽然NAA诱导的效果较好,但愈伤组织生长速度较慢;而2,4-D则能高效地诱导出愈伤,但所诱导的愈伤容易出现玻璃化现象。提高NAA,2,4-D和BA的浓度,愈伤呈现水浸状的比例和程度都会相应的提高。所设计的组合中,2,4-D 0.1mg/l+BA 0.3 mg/l对胚轴愈伤的诱导效果较好。③暗培养能提高愈伤组织的生长量.随着黑暗培养时间的延长,愈伤组织松散程度也不断上升,子叶颜色由浓绿色逐步向淡黄色过渡。
1.2.2愈伤组织的继代培养
①在固体培养基中,子叶愈伤组织继代培养可以根据目的不同选取不同的继代培养基。长期继代培养,将愈伤组织继代培养在NAA1.0mg/l+KT0.5mg/l的培养基或NAA0.5 mg/l与TDZ 2mg/l的组合中,可得到较好的效果。如需短期内获得较多的愈伤组织,可将愈伤组织在2,4-D1.0mg/l+KT0.1mg/l中培养。②子叶愈伤在液体培养条件下,生长量较低,效果较差。③胚轴愈伤在NAA1mg/l+KT 0.3mg/l培养基中的生长量最大。④维生素C对愈伤组织降低褐化的作用较小,但能促进愈伤组织变绿。
1.3不定芽的诱导发生:
子叶诱导不定芽的培养基中需要添加一定量的TDZ和NAA。不定芽有玻璃化现象,在TDZ 0.5 mg/l + NAA 1.0mg/l的培养基中得到的不定芽发育较好。
2.不同NaCl浓度对种子和愈伤组织的生长的影响,主要结论如下:
2.1愈伤组织:当NaCl浓度较低时,愈伤组织的生长量未受到较大影响、MDA含量较低,POD、SOD活性提高,细胞内可溶性糖、脯氨酸含量升高。而在高浓度NaCl的胁迫下,愈伤组织的生长具有明显的抑制作用、MDA含量升高,而POD、SOD活性开始降低、可溶性糖含量也降低,但是脯氨酸含量一直升高。
2.2幼苗
随着NaCl浓度的升高,种子的萌发率和幼苗根系活力呈递减趋势;除了在0.5‰NaCl处理的幼苗中,其叶绿素含量高于对照,其余处理均低于对照;幼苗的SOD、POD活性变化趋势较一致,先升高后降低,在1‰NaCl浓度具有最好的活性。MDA含量随NaCl处理浓度的升高而升高。
Take the seeds of Chaenomeles speciosa Nakai as plant material; we carried out researches about tissue culture and salt tolerant.
1. The most important conclusion about tissue culture, as follows:
1.1 Multiply and root of adventitious:
Compared KT and TDZ, BA has a better effect on multiply adventitious buds of Chaenomeles speciosa Nakai. The effect of NAA and IBA on multiply adventitious buds is similar. The explants cultured on medium supplemented with BA0.5 mg/l +NAA0.2 mg/l have the highest multiply ability. Reducing the content of MS macro element has a negative effect on multiply adventitious buds. The adventitious buds cultured on medium contained 1/2MS+IBA0.2 mg/l have highest induction root rate, and inducted roots is longer than other combination medium.
1.2 Induction and subculture of callus tissue:
1.2.1 Callus induction
①In the experiment of cotyledons, 2,4-D has a better effect on inducing and subculture callus tissue than that of NAA. A satisfactory result of callus tissue induction and callus tissue growth was achieved on MS medium supplemented with 1mg/l 2,4-D and 0.1mg/l KT.②Callus tissue induction rate of hypocotyls was 87%, and it was higher than that of cotyledons which was 69%. The proportion and the degree of callus vitrifaction would scaled up as increasing the concentration of auxin and BA. Best callus of hypocotyls were obtained on MS medium contained 0.1mg/l 2,4-D and 0.3mg/l BA .③Dark culture incentives callus tissue growth. Longer the days of dark culture, the looser of callus tissue. The colors of cotyledons transferred from dark green to light yellow with increasing dark culture.
1.2.2 Subculture of callus tissue
①On solid medium, the subculture of cotyledons has different culture methods. If the subculture with long time, callus tissue was cultured on medium supplemented with NAA1.0mg/l+KT0.5mg/l or NAA0.5 mg/l + TDZ 2mg/l, which can obtain better result. On the other hand, callus tissue can growth quickly on medium contained 2,4-D 0.1 mg/l +BA 0.3mg/l and we can get more callus tissue in a short time.②Cultured in liquid medium, callus tissue of cotyledons grew slowly.③Callus tissue of hypocotyls growth most rapidly when cultured on medium contained NAA1mg/l+KT0.3mg/l.④The effect of Vc on reducing browning of callus tissue of Chaenomeles speciosa Nakai was less, but promoted colors of callus tissue turn to green.
1.3 Induction adventitious buds
Induction adventitious buds from cotyledons, it was essential that added some TDZ and NAA into induction medium. Some adventitious buds were vitrifaction, and the adventitious buds were growth normally when induced from the medium
supplemented with TDZ 0.5 mg/l + NAA 1.0mg/l.
2. The effect of different concentration NaCl on the growth of seeds and callus tissue, the main conclusion as follows:
2.1 Callus tissue
In low concentration of NaCl, the growth of callus tissue kept normally level、the content of MDA was lower than check, and the activity of POD and SOD higher than check, the content of soluble sugar and Pro was higher than check. When cultured on a high NaCl concentration, the growth of callus tissue reduced rapidly, the content of MDA was increased, and the activity of POD and SOD reduced; the content of soluble sugar also reduced, but the content of Pro was still increased.
2.2 Seedings
With the increasing of NaCl concentration, the germination of seeds and activity of root system was reduced; except the seeds growth in 0.5‰NaCl, the content of seeds leave’s chlorophyll in the others treatment was lower than blank. Firstly the activity of plantlets’SOD and POD increased, subsequently decreased, and highest enzyme activity was obtain when the seeds cultured on medium contained 1‰NaCl. The content of MDA was scaled up with the increasing of NaCl concentration.
引文
[1]曹慧娟.植物学(第二版) [M].中国林业出版社,北京,1989:10
[2]周维燕.植物细胞工程原理与技术[M].中国农业大学出版社.北京,2001:39
[3]吴兴海.虎掌和猕猴桃组织培养的研究[D].陕西师范大学硕士论文,2003,1-10
[4]Steward FC, Mapes M0, Mears K. Growth and organized development of cultured cells, I. Organization in cultures from freely suspended cells[J]. Am J Bot, 1958, 45:705-708
[5].Zenk M H. Haploids in physiological and biochemical research[J]. In Haploids in Higher plants Advances Potential, 1974, (3):39-354
[6].裴翠绢,李洪杰,郭北海,等.冬小麦幼穗无性系变异分析[J].河北农业科学 1997,1(3):11-15
[7].周荣仁,杨燮荣,余叔文.利用组织培养研究植物耐盐机理与筛选耐盐突变体的进展[J].植物生理学通讯,1989,(5):11-19
[8]Nabors M W. In Randall DC(ed) Current Topics in plant biochemistry and physiology[J]. Uni of Missouri Columoia, 1983,.3:165.
[9]Skoog F, Miller CO. Chemical regulation of growth organ formation in plant tissue cultured in vitro[J]. Symp Soc Exp Biol, 1957, 11:118-131
[10]潘瑞炽.植物生理学(第 5 版) [M].高等教育出版社.北京,2004
[11]吴玮琳,叶文法,杨曼丽等.芳双酰胺衍生物合成及植物生长调节活性研究[J]. 武汉理工大学学报, 2004, 26(6):15-17
[12]Thorpe TA. Organogenesis in vitro: structural, physiological and biochemical aspects. In: Vasil IK (ed) Perspective in plant cell and tissue culture[M].A. Academic Press, New York,1980:71–105
[13]Yeung EC. Structural and developmental patterns in somatic embryogenesis[M]. In vitro embryogenesis in plants. Kluwer, Dordrecht, 1995: 205–247
[14]Y-H. Wang , P. L. Bhalla. Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br. [J] Plant Cell Rep, 2004, 22:408–414
[15]陈正华.木本植物组织培养及其应用[M].北京:高等教育出版社,1986
[16]Benelli C, Fabbri A, Grassi S, Lambardi M, Rugini E. Histology of somatic embryogenesis in mature tissue of olive (Olea europaea L.) [J]. J Hortic Sci Biotechnol, 2001,76:112–119
[17]Tisserat B. Embryogenesis, organogenesis and plant regeneration[M]. In: Dixon RA (ed) Plant cell culture: a practical approach. IRL Press, Eynsham, 1985:79–104
[18]傅立国,陈潭清,郎楷永,等主编.中国高等植物(第 6 卷)[M].山东:青岛出版社,2003:555-557
[19]吴国梁.不同激素对皱皮木瓜嫩茎段离体培养的影响[J].山西林业科技 1993,3:35-38
[20]范义莲,万益琴,刘志学.植物组织培养简报摘编[J],植物生理学通讯,2004,40(5):594
[21]程玉琴,韩振海,徐雪峰.苹果病毒及其脱毒检测技术研究进展[J],中国农学通报第 19 卷第 1 期 2003年 2 月:72-96
[22]洪艳华,殷广峰,张立军.百合脱毒及病毒检测技术研究进展[J].沈阳农业大学学报.2003,2006,34 (3):225- 227
[23]杨林栋.郭瑞锋.皇甫红芳.组织培养在马铃薯育种上的研究进展[J].中国马铃薯,2004,18(4):224-227
[24]伍成厚,潘一山,罗开梅等.蝴蝶兰未受精胚珠离体培养的研究[J].园艺学报2006,33(4):891～894
[25]金晓霞 张启翔.报春花属植物的育种研究进展[J],植物学通报.2005,22(6):738～74
[26]周朴华,何立珍,刘选明.组织培养中用秋水仙素诱发黄花菜同源四倍体的研究[J].中国农业科学, 1995 ,28(1):49~55
[27]何立珍,周朴华,刘选明,等.南荻同源四倍体的研究[J].遗传学报,1997,24(6):544~549
[28]吴伟刚,刘桂茹,杨学举..诱变与组织培养相结合在植物育种中的应用[J],中国农学通报.2005,21(11): 197-201
[29]张恩让,程智慧,周新民. 大蒜体细胞无性系的染色体[J]. 西北农林科技大学学报(自然科学版).2004, 32(9): 73-76
[30]Kirby, Edward. Plant Cell Cultures[J]. Bioscience; ProQuest Biology Journals,1989,39(9):645-646
[31]Ken J.Kasha. Biotechnology and world food supply. Genome; Aug 1999; 42(4):642-645
[32]孙振晓,马清温. 抗癌植物药及其植物组织培养研究进展[J].生物学通报,2000,35(8): 11-12
[33]MII M, OHASHIH.Plandet regeneration from protoplasm of kiwifruit,Actinidia chinensis planeh[J].Japan J Breed,1998,38:90-97.
[34]秦永华,张上隆,朱道圩.猕猴桃的组织培养和遗传转化研究进展[J].细胞生物学杂志, 2004,26(1):57-61
[35]桂耀林,徐延玉,母锡金.猕猴桃胚乳培养中的胚胎发生[J].武汉植物学研究,1988,6(4):395-397
[36]Nakano H.Plant differentiation in callus tissue induced from immature endosperm of Oryzasativa L Z[J]..Pflanzphysiol, 1975,76(5):444-449
[37]Progamme et al. Ⅷ Congress of the lnterational Society of Citriculture,Sun City Resort,South Africa, 1996,12:7
[38]Yang Xiaoling,Kitajima A,Hasegawa.ca1lus induction and Embyoid regeneration from the endosperm culture of ‘Tosa Buntan’ pummelo[J].Environment Control in Biology,2000,38(4):24l-246
[39]刘明,赵琦.高梁组织培养研究进展及关键技术[J].首都师范大学学报(自然科学版),2005,26(4): 60-63
[40]刘云国,王晓云.苹果种质资源玻璃化法超低温保存技术[J].山东农业大学学报(自然科学版),2002, 33(1):32-36
[41]中国科学院自然资源综合考察委员会.中国1:100 万土地资源图土地资源数据集[M],北京:中国人民大学出版社,1991
[42]叶笃正,陈泮勤主编. 中国的全球变化研究[M]. 北京:地质出版社,1992. 187-208
[43]赵可夫,李法曾主编.中国盐生植物[M].北京:科学出版社,1999.1,43-47
[44]FLOWERS, T. J.; TROKE, P. F. & YEO, A. R.: The mechanism of salt tolerance in halophytes. Annu. Rev. Plant Physiol. 1977,28:89-121
[45]MAAS, E. V. & NIEMAN, R. H.: Physiology of plant tolerance to salinity. In: JUNG, G. A. (ed.): Crop tolerance to suboptimal land conditions. Agron. Soc. America, Crop Sci. Soc. America and Soil Sci. Soc. America, Madison WI, 1978,277-299
[46]GORHAM, J.: Mechanism of salt tolerance of halophytes. In: CHOUKR-ALLAH, R., MALCOLM, C. V. & HAMDY, A. (eds.) : Halophytes and biosaline agriculture. Marcel Dekker, New York, 1995,207-223
[47]MUNNS, R.: Physiological processes limiting plant growth in saline soils: some dogmas and hypotheses[J]. Plant Cell Environ. 1993,16: 15-24.
[48]Muhammad Ashraf.:Some important physiological selection criteria for salt tolerance in plants[J]. Flora. Jena: 2004,199 (5):361-376
[49]许兴,毛桂莲,李树华等.NaCl 胁迫和外源 ABA 对枸杞愈伤组织膜脂过氧化及抗氧化酶活性的影响[J].西北植物学报,2003,23(5):745-749
[50] 陈利萍,徐春霞,李春顺.影响茎用芥菜愈伤组织诱导和植株再生的因素[J].植物生理学通讯,2005,41 (6): 758-760.
[51] Unnikrishnan, S. K.; Prakash, L.; Josekutty, P. C.; Bhatt, P. N.; Mehta, A. R. Effect of NaCl salinity on somatic embryo development in Sapindus trifoliatus L[J]. J. Exp. Bot. 1991,42: 401-406
[52] Beloualy, N; Boubarmont, J. NaCl-tolerant plants of Poncirus trigoliata regenerated from tolerant cell lines[J]. Theor. Appl. Genet. 1992,83:509-614
[53]Kumar, V.; Sharma, D. R. Isolation and characterization of sodium chloride-- resistant callus culture of Vigna radiata (L.) Wilczek var. radiata[J]. J. Exp. Bot. 1989 ,40:143-147
[54]Ericson. M. C. and Alfinito, S.H. Plant Physiol., 1984, 74:506
[55]Singh, N.K., Handa, A.K., HASEGAWA,p.m.et al., Plant Physiol,1985,79:126
[56] 陆 卫 , 贾 敬 芬 . 谷 子 胚 性 愈 伤 组 织 耐 盐 系 的 选 择 及 其 生 理 生 化 特 性 分 析 [J]. 作 物 学 报 1994,20(3):241-247
[57]张可炜,王先艳,王灵芝,等.利用细胞工程技术培育玉米耐盐自交系及单交种[J].山东农业科学,2001,6:15-17
[58]王鸣刚,陈亮,贾敬芬.耐盐突变体小麦后代耐盐稳定性分析研究[J]. 武汉植物学研究,2004, 22 (1):39-43
[59] 杜立群,李银心,李洪杰,等.在1/3海水培养基上筛选豆瓣菜耐盐变异体[J].植物学报1999,41 (6): 633-639
[60]M. Zacchini · A. Marotta · M. de Agazio.Tolerance to salt stress in maize callus lines with different polyamine content[J]. Plant Cell Reports 1997, 17: 119–122
[61]Y.MIKI,M.HASHIBA, S.HISAJIMA.Establishment of salt stress tolerate rice plants through step up NaCl treatment[J] in vitro.Biologia plantrum 2001,44(3):391-395
[62]岳玮,夏光敏,陈惠民,等.獐毛愈伤组织耐盐性研究及耐盐变异体的筛选[J].山东大学学报(自然科学版) 2000,35(3): 338-343
[63]郑霞,韦小敏,季良越,等.玉米体细胞抗盐突变体的筛选及耐盐性鉴定[J].河南农业大学学报 2004,38(2):139-143
[64]贺道耀,余叔文.水稻高脯氨酸变异系高脯氨酸含量和耐盐性的遗传性[J].植物生理学报 1997,23(4):357-362
[65]米海莉,许兴,李树华,等.春小麦耐盐突变体的筛选及耐盐性的研究[J].宁夏农林科技 2001,3:1-4
[66]祁翠兰,李志勇,刘秉信.红豆草耐盐突变体的研究[J].中国草地 1998,3:44-46
[67]郑文菊,王勋陵,贾敬芬.小麦耐盐系愈伤组织细胞的超微结构观察[J].西北植物学报 1999,19(1):81-84
[68]Smith M M,Hodson M J. Salt-induced ultrastructural damage to mitochondria in root tips of asalt-sensitive ecotype of Agrostisstolonifera[J]. J. Exp.Bot.,1982,33 (136) :886-895
[69]裴翠绢,李洪杰,郭北海,等.冬小麦幼穗无性系变异分析[J].河北农业科学 1997,1(3):11-15
[70]贾恢先,赵曼容,马莹.典型盐地植物细胞脂质过氧化伤害与质膜超微结构变化的研究[J].西北植物学报1994,14(6):1-5
[71]张恩让,程智慧.大蒜耐盐愈伤组织变异系的选择研究[J].西北植物学报 2003,23(9):1571-1576
[72]曾斌,罗淑萍,李疆.新疆野生巴旦杏的组织培养和植株再生[J].新疆农业大学学报,2006,29 (4) :27～31
[73]赵可夫,范海,宋杰,等.中国盐生植物的种类、类型、植被及其经济潜势.盐生植物利用与区域农业可持续发展[M].北京:气象出版社.2002
[74]彭峰,陈嫣嫣,郝日明等.彩色马蹄莲‘Parfait’不定芽诱导增殖培养条件的优化和筛选[J].植物资源与环境学报,2006, 15 (2) : 47 - 49
[75]陈金慧,施季森,诸葛强.杂交鹅掌楸的不定芽诱导及植株再生[J].植物生理学通讯, 2002,38(5):459
[76]李际红,谢会成,许建农等.穿山薯蓣不定芽的诱导与植株再生[J].山东农业大学学报(自然科学版) , 2005, 36 (1) : 82-85
[77]Samir C. Debnath.Clonal propagation of dwarf raspberry (Rubus pubescens Raf.) through in vitro axillary shoot proliferation[J].Plant Growth Regulation, 2004,43:179–186
[78]李林,李科友,唐德瑞.白皮松离体胚培养与不定芽的诱导[J].西北林学院学报,2004, 19 (2) : 61- 63
[79]陆瑞菊, 黄剑华, 王亦菲等.三倍体毛白杨快繁技术研究[J].中国农学通报,2004,19(2): 80-82
[80]武宗信,解红娥,冯文龙等.地黄脱毒技术研究[J].中药材,2002,25(6):383-385
[81]宋建英,叶建仁.“马可”百合组培技术研究[J]. 南京林业大学学报(自然科学版) ,2006 ,30(7):73-76
[82]周瑞金,刘孟军.枣离体叶片高效再生植株的研究[J].园艺学报,2006, 33 (3) : 625～628,
[83]王鸿,马锋旺,郝,燕等.山杏下胚轴再生植株的研究[J].西北农林科技大学学报(自然科学版),2005,33 (5):137-130
[84]王光萍,丁雨龙,黄敏仁等.观赏竹的试管快繁研究[J].林业科学,2005,41(5):51-55
[85] 陈 菊 , 陈 国 惠 . 何 首 乌 生 根 培 养 因 子 的 优 选 研 究 [J]. 西 南 农 业 大 学 学 报 ( 自 然 科 学 版 ) 2006,28(5):576-578
[86]曾炳山,刘英,许煌灿等.长嘴黄藤离体快繁研究[J].福建林学院学报,2002, 22 (2) : 169-171
[87]张超,陈奉玲,汤兴毫.木瓜的本草考证[J].中草药,1999,30(12):943.
[88]吴延廷,张俊.中药木瓜的基源和性状鉴定[J].华西医科大学学报,1996,27(4):404- 408.
[89]洪永福,郭学敏.三种木瓜的乙醚提取部位的气相色谱-质谱分析[J].第二军医大学学报,2000, 21(8):749-752.
[90]潘瑞炽.植物组织培养[M].北京:科学出版社,2003.
[91]刘先勇,袁长迎,段宝福等.SPSS10.0统计分析软件与应用[M].北京:国防工业出版社,2002.
[92]符文英,杜道林,符木均.海南粗榧愈伤组织的诱导和培养[J].植物生理学通讯,2004 ,40(1):33-36
[93]许忠民,张恩慧,欧承刚,等.不同因素对甘蓝花药愈伤组织诱导的影响[J].西北农林科技大学学报(自然科学版) . 2006,34(9): 65-68
[94]陈永勤,朱蔚华,吴蕴祺等.云南红豆杉细胞培养和紫杉醇生产[J].湖北大学学报(自然科学版),2001,23(4):366-369
[95]M. K. Kim ,H. E. Sommer,B. C. Bongarten,S. A. Merkle.High-frequency induction of adventitious shoots from hypocotyl segments of Liquidambar styraciflua L. by thidiazuron[J]. Plant Cell Reports,1997,16: 536–540
[96]魏小勇.铁皮石斛原球茎悬浮培养研究[J].现代中药研究与实践,2004,18(4):7-11
[97]王镜岩,朱圣庚,徐长法.生物化学[M].高等教育出版社,北京,2002
[98]刘贵周,谢世清,赵庆云登.优质魔芋组培快繁技术研究[J].云南农业大学学报,2005,20(6):795-799
[99]李玉平,王永宏,张强等.大花金挖耳无菌外植体的获得及愈伤组织诱导的初步研究[J].西北农林科技大学学报(自然科学版),2006,34(10):171-176
[100]盛长忠,王淑芳,王宁宁等.红豆杉愈伤组织培养中褐变现象的初探[J].南开大学学报(自然科学),2001,34 (4): 120-122
[101]邱璐,王波,王志和等.史密斯桉愈伤组织的诱导及分化[J].东北林业大学学报, 2006,34(4):18-21
[102]李琳,叶春.影响库拉索芦荟(Aloevera L.)愈伤组织诱导的因素[J].四川大学学报(自然科学版), 2003,40(5): 963-965
[103]钱永强,孙振元,李云等.野牛草成熟胚植株再生及其影响因素研究[J].草业科学,2005,22(2):101-106
[104]陈芳清,丘安机,徐祥浩.药用植物红芽大戟的组织培养[J].广西植物, 1997,17(2):149-151
[105]王雅英,杨忠耿,陈汉鑫等.新台糖25号甘蔗愈伤组织诱导试验[J].亚热带植物科学,2003,32(4):30-32.
[106]袁鹰,刘德璞,郑培和等.钢东北玉米自交系胚性愈伤组织的诱导[J].玉米科学,2001,9(1):37-38
[107]袁红霞,王金虎,陈德燕等.何首乌块根愈伤组织培养[J].苏州大学学报(自然科学版) ,2005,21(13): 86-88
[108]王建华,王义,孙国伟等.人参组织培养的多因子正交试验研究[J].吉林农业大学学报, 2006,28(16):468-451
[109]朱靖杰,王宇光,雷禄旺等.皇帝蕉薄片外植体愈伤组织的诱导及植株再生[J].果树学报,2006, 23(1):111-114
[110]陈芳清,丘安机,徐祥浩.药用植物红芽大戟的组织培养[J].广西植物, 1997,17(2):149-151
[111]王雅英,杨忠耿,陈汉鑫等.新台糖25号甘蔗愈伤组织诱导试验[J].亚热带植物科学,2003,32(4):30-32
[112]洪岚,叶万辉,沈浩等.薇甘菊组织培养及体细胞胚胎发生的研究[J].浙江大学学报(农业与生命科学版),2005,31 (5) : 572-578
[113]乔琦,肖娅苹,王喆之.外源激素对防风体细胞胚发生和发育的影响[J].西北大学学报(自然科学版),2005,35(3): 316-320.
[114]陈利萍,徐春霞,李春顺.影响茎用芥菜愈伤组织诱导和植株再生的因素[J].植物生理学通讯,2005,41(6):758-760.
[115]陆美莲,许新萍,周厚高等.均匀正交设计在百合组织培养中的应用[J].西南农业大学学报(自然科学版).2004,26 (6):699-703.
[116]陈火英,张建华,俞俊棠等.番茄离体培养过程中器官发生的细胞组织学观察[J].武汉植物学研究,2001,19(2): 91-95
[117]皮伟.几个因素对草地早熟禾种子愈伤组织诱导的影响[J].西南农业大学学报(自然科学版),2006,28(5): 747-749
[118]韩珊,石大兴,王米力等.红叶乌桕茎段离体培养的研究[J].四川农业大学学报,2006,24(3):301-302
[119]白文苑,沈慧敏.苍耳愈伤组织诱导及继代培养研究[J].甘肃农业大学学报,2006,41(1):65-68
[120]刘航空,韩明玉,禹婷等.影响油桃叶片产生胚性愈伤组织的因素[J].果树学报,2006, 23( 3):370-374
[121]李毅,邸利,洪涛等. 箭胡毛杨愈伤组织诱导、保存与再分化[J].西北植物学报,2002,22(3): 656-600
[122]姜蕾,兰天维,黎扬辉等.影响红掌愈伤组织诱导、增殖和芽分化的因素[J].种子, 2006,25(11): 26-30
[123]马均,马明东,周宇.爝曼地亚红豆杉愈伤组织诱导试验[J].林业科技,2006,31(1):12-14
[124]刘金英,徐有明,李双来等.佛手山药组织培养的研究[J].植物研究,2006,26(3):323-328
[125]韩清霞,沈火林,朱鑫等.芹菜胚性愈伤的诱导及高频植株再生体系的建立[J].中国蔬菜,2006 (11) : 6～9
[126]龚伟,胡庭兴,宫渊波等.光叶子花茎段愈伤组织的诱导及其植株再生的研究[J].园艺学报,2005,32 (6):1125-1128
[127]肖荷霞,王瑛,高峰等.外植体及激素对SANDITI紫花苜蓿愈伤组织诱导和分化的影响[J].河北农业大学学报, 2003,26(4):47-52
[128]毛堂芬,黄先群.少花桂愈伤组织诱导的初步研究[J].种子,2005,24(8):75-76
[129]彭晓莉,王蒂,张金文等.激素诱导下不同培养方式对马铃薯微型薯的诱导效应[J].甘肃农业大学学报,2006, 41(1):16-19
[130]闫桂琴,田丽宏,杨利艳.濒危植物翅果油树愈伤组织诱导中褐变问题的研究[J].西北植物学报,2004, 24(8):1384-1389
[131]黄远新,何凤发,张盛林.魔芋组织培养与快繁技术研究[J].西南农业大学学报(自然科学版),2003,25(4): 309-312
[132]高蓉,赵博光.防止黑松外植体及其愈伤组织褐变的方法[J].南京林业大学学, 2001,25(5):75-77
[133]付成华,沈宝仙.I-72杨再生体系的建立[J].华中农业大学学报,2006,25(5):564-567
[134]刘国诠.生物工程下游技术[M].化学工业出版社,北京,2003
[135]王志强,王惠林,廖新福等.新疆哈密瓜子叶不定芽诱导与植株再生[J].新疆农业大学学报,2005,28 (3):79-81
[136]金宝燕,苏华,任华中.影响黄瓜直接不定芽诱导的品种等因素研究[J].中国农学通报, 2006,22(6):45-48
[137]Chalupa V, Large scale micropropagation of Quercus roburL. using adenine-type cytokinins and thidiazuron to stimulateshoot proliferation[J]. Bio Plant,1988,30:414–421.
[138]Lu C-Y. The use of thidiazuron in tissue culture[J]. In Vitro Cell Dev Biol,1993,29:92–96
[139]Van Nieuwkerk JP, Zimmerman RH, Fordham I (1986) Thidiazuron stimulation of apple shoot proliferation[J] in vitro. HortScience 21:516–518
[140]Yusnita S, Geneve RL, Kester ST, Micropropagation of white flowering eastern redbud (Cercis canadensis var ‘alba’ L.) [J]. J Environ Hortic ,1990, 8:177–179
[141]Murat ?zgen, Sebahattin ?zcan1_, Cafer S. Sevimay, Cengiz Sancak & Mustafa Y?ld?z High frequency adventitious shoot regeneration in sainfoin[J].Plant Cell, Tissue and Organ Culture,1998, 52: 205–208
[142]Ben A. Bergmann,Heung-Kyu Moon.In vitro adventitious shoot production in Paulownia[J]. Plant Cell Reports,1997, 16:315-319
[143]Wei Tang, Fan Ouyang.Plant regeneration via organogenesis from six families of loblolly pine[J]. PlantCell, Tissue and Organ Culture,1999,58:223–226,.
[144]张国裕,程智慧,李娟等.银条不定芽诱导及高效增殖体系的建立[J].西北农林科技大学学报(自然科学版), 2004,32 (2):49-52
[145]师校欣,杜国强,马俊莲等.磨盘柿离体叶片愈伤组织发生及不定芽诱导[J].果树学报2004, 21(4): 376-378
[146]Ting-Yu Chen, Jen-Tsung Chen ,Wei-Chin Chang.Plant regeneration through direct shoot bud formation from leaf culturesof Paphiopedilum orchids[J].Plant Cell, Tissue and Organ Culture,2004, 76:11–15
[147]田宏现,曾艳玲,谭晓风等. 米良一号猕猴桃的组织培养研究[J].经济林研究,2005,23 (1):7-9
[148]Chun-Lai Zhang; Dong-Fang Chen; Malcolm C Elliott et al.Efficient procedures for callus induction and adventitious shoot organogenesis in sugar beet (beta vulgaris l.) breeding lines[J]. In Vitro Cellular & Developmental Biology,2004, 40(5):475-481
[149]汤浩茹,刘翠琼,罗娅.满园香梨叶片不定芽的诱导[J].果树学报,2005,22(6):706-708
[150]Y.E. Choi,D.-C. Yang,E.-S. Yoon etal.Plant regeneration via adventitious bud formation from cotyledon explants of Panax ginseng C.A. Meyer[J] Plant Cell Reports,1998, 17: 731–736
[151]Eunju Cheong,Margaret R Pooler.Micropropagation of Chinese redbud (cercis yunnanensis) through axillary bud breaking and induction of adventitious shoots from leaf pieces[J].In vitro cellular & developmental biology. 2003,39(5):455-458
[152]A.K. Kobayashi , J.C. Bespalhok , L.F.P. Pereira,L.G.E. Vieira1, Plant regeneration of sweet orange (Citrus sinensis) from thin sections ofmature stem segments[J].Plant Cell, Tissue and Organ Culture ,2003 74: 99–102
[153]Cheng Zhu,Zhen Chen.Role of polyamines in adventitious shoot morphogenesis from cotyledons of cucumber[J]. In vitro Plant Cell Tiss Organ Cult,2005, 81:45–53
[154]G. Liu,M. Bao.Adventitious shoot regeneration from in vitro cultured leaves of London plane tree (Platanus acerifolia Willd.) [J].Plant Cell Rep,2003,21:640–644
[155]LARKIN P J , SCOWCROFTW R. Somaclonal variation-anovel source of variablity from cell cultures for plant improvement [J]. Theor. App. Genet, 1981, 60: 117-124.
[156]SALINML Toxic oxygen species and protective systems of the chloroplast[J]. Physiol.Plant, 1988,72:681-689.
[157]魏玉清,许兴,王璞.土壤盐胁迫下宁夏枸杞的生理反应[J].中国农学通报,2009,21(9)213-217
[158]刘凤荣,陈火英,刘杨等.盐胁迫下不同基因型番茄可溶性物质含量的变化[J].植物生理与分子生物学报,2004 ,30 (1):99-104
[159]赵可夫.植物抗盐生理[M ].北京:中国科学技术出版社, 1993: 24 - 27, 230, 231.
[160]Rosario Vera-Estrella1, Bronwyn J. Barkla1, Hans J. Bohnert2, Omar Pantoja1 Salt stress in Mesembryanthemum crystallinum L. cell suspensions activates adaptive mechanisms similar to those observed in the whole plant[J], Planta, 1999, 207: 426-435
[161]赵素然,梁宇,李德森等.毛白杨愈伤组织悬浮细胞耐盐性研究[J].广西植物,2000,20 (4) : 351-355
[162] 杨 素 欣 , 王 振 镒 . 盐 胁 迫 下 小 麦 愈 伤 组 织 生 理 生 化 特 性 的 变 化 [J]. 西 北 农 业 大 学 学报.1999,27(2):48-51
[163]齐志广,杨献光,徐涛等.盐胁迫对不同基因型小麦成熟胚愈伤组织生长的影响[J].2004,28(1):河北师范大学学报(自然科学版):68-71
[164]武维华.植物生理学[M].科学出版社,北京,2003
[165]张美云, 钱吉, 钟扬等.野生大豆若干耐盐生理指标的研究[J].复旦学报(自然科学版),2002,41(6): 669-673
[166]Ireneusz S lesak, Marta Libik & Zbigniew Miszalski. Superoxide dismutase activity in callus from the C-CAM intermediate plant Mesembryanthemum crystallinum[J]. Plant Cell, Tissue and Organ Culture ,2003,75: 49–55,
[167]Barbara Karpinska, Marlene Karlsson, Helga Schinkel et al.A Novel Superoxide Dismutase with a High Isoelectric Point in Higher Plants[J]. Plant Physiology, 2001, 126:1668–1677
[168]SONG Fu-nan, YANG Chuan-ping, LIU Xue-mei, LI Gong-bin.Effect of salt stress on activity of superoxide dismutase (SOD) in Ulmus pumila L[J]. Journal of Forestry Research, 2006,17(1): 13–16
[169]宋福南,杨传平,刘雪梅等.盐胁迫对怪柳超氧化物歧化酶活性的影响[J].东北林业大学学报,2006,34(5):54-56
[170]魏爱丽,白桦,陈云昭等.盐胁迫下大豆初生叶愈伤组织SOD活性及其同工酶变化的研究[J].大豆科学,1999,18(1): 85-88
[171]贺岩,李志岗,李新鹏,赵娟.盐胁迫条件下两种基因型小麦生长及保护酶活性的反应[J].山西农业大学学报, 2005,25 (1):42-44
[172]Yardena Gueta-Dahan, Zohara Yaniv, Barbara A. Zilinskas, Gozal Ben-Hayyim. Salt and oxidative stress: similar and specie responses and their relation to salt tolerance in Citrus[J].Planta ,1997,203: 460-469
[173]谭大海,沙伟,张莹莹.芦苇盐胁迫下渗透调节物质含量变化研究[J].齐齐哈尔大学学报,2006,22(2):84-86
[174]卢静君,多立安,刘祥君.盐胁迫下两草种SOD和POD及脯氨酸动态研究[J].植物研究, 2004,24(1): 115-119
[175]刘会超,孙振元,彭镇华.NaCl胁迫对五叶地锦生长及某些生理特性的影响[J].林业科学,2004,40(6):63 -67
[176]万劲,石雷,张金政等.盐胁迫对鸢尾叶片生理指标的影响[J].南京林业大学学报(自然科学版)2006,30 (1):57-60
[177]Valentine RC.Californian Agriculture,1984,38(10):36
[178]李卫欣,陈贵林,赵利等.NaCI 胁迫下不同南瓜幼苗耐盐性研究[J].植物遗传资源学报2006 ,7 (2): 192-196
[179]费伟,陈火英,曹忠等.盐胁迫对番茄幼苗生理特性的影响[J].上海交通大学学报(农业科学版第), 2005,23(1)-5-11
[180]Sangita Basu, Gaurab Gangopadhyay,B.B. Mukherjee. Salt tolerance in rice in vitro: Implication of accumulation of Na+, K+ and praline[J].Plant Cell, Tissue and Organ Culture 2002, 69: 55–64
[181]裘丽珍,黄有军,黄坚钦等.不同耐盐性植物在盐胁迫下的生长与生理特性比较研究[J].浙江大学学报(农业与生命科学版), 2006,32 (4) : 420-427
[182]李国雷 ,孙明高,夏阳等.NaCl 胁迫下黄栌、紫荆的部分生理生化反应动态变化规律的研究[J].山东农业大学学报(自然科学版) , 2004 , 35 (2) : 173-176
[183]吴能表,叶腾丰,,王小佳.NaCl胁迫对豌豆幼苗生理生化指标的动态影响[J].西南农业大学学报(自然科学版),2006, 28(1): 37-40
[184]王征宏,杨起,张亚冰.盐胁迫下紫花苜蓿种子的萌发特性[J].河南科技大学学报(自然科学),2006,27(1): 67-80
[185]郇树乾,刘国道,张绪元等.NaCl胁迫对刚果臂形草种子萌发及幼苗生理效应的研究[J].中国草地, 2004,26(6) : 45 - 49.
[185]尹增芳,何祯祥,王丽霞,钦佩.NaCl 胁迫下海滨锦葵种子萌发和幼苗生长过程的生理特性变化[J].植物资源与环境,2006(1):14-17
[186]Romero Aranda R, Soria T,Cuartero J.Tomato plant-water uptake and plant-water relationships under saline growth conditions [J]. Plant Science, 2001, 160: 265 - 272.
[187]Wang Y, NilN. Changes in chlorophyll, ribulose biphosphate carboxylase - oxygenase, glycine betaine content, photosynthesis and transpiration in Amaranthus tricolor leaves during salt stress[J]. J Hortic SciBiotechnol, 2000, 75: 623 - 627.
[188]於丙军,李锁娜,刘友良.大豆苗期盐害离子效应的比较[J].南京农业大学学报, 2002, 25 (1):5 - 9.
[189]王仁雷,华春,刘友良.盐胁迫对水稻光合特性的影响[J].南京农业大学学报, 2002, 25 (4) : 11 - 14.
[190]董晓霞,赵树慧,孔令安,等.苇状羊茅盐胁迫下生理效应的研究[J].草业科学, 1998,15 (5) : 10-13.
[191]董晓霞,郭洪海,孔令安.杂交酸模耐盐性鉴定的生理指标筛选[J].山东农业科学, 1999 (6) : 22 - 24
[192]刁丰秋,章文华,刘友良.盐胁迫对大麦叶片内囊体膜组成和功能的影响[J].植物生理学报,1997,23(2):105-110
[193]夏阳,孙明高,李国雷等.盐胁迫对四园林绿化树种叶片中叶绿素含量动态变化的影响[J].山东农业大学学报(自然科学版),2005,36 (1):30-34
[194]朱进,别之龙,李娅娜等.盐胁迫对不同基因型黄瓜幼苗生长和光合作用的影响[J].沈阳农业大学学报. 2006- 06.37(3):476- 478
[195]Santos C, Caldeira G. Comparative responses of Helianthus annuus plants and calli exposed to NaCl:. Growth rate and osmotic regulation in intact plants and calli[J]. J Plant Physiol, 1999, 155: 769 - 777.
[196]Santos C, Azevedo H, Caldeira G. In situ and in vitro senescence induced by KCl stress: nutritional imbalance, lip id peroxidation and antioxidantmetabolism[J]. J Exp Botany, 2001: 52: 351 - 360.
[197]Santos C. Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves[J]. Scientia Horticultures, 2004, 103: 93 - 99.
[198]刘家尧,衣艳君,张其德.盐胁迫对不同抗盐性小麦叶片荧光诱导动力学的影响[J].植物学通报,1998, 15 (2):46 - 49.
[199]Rao G G, Rao G R. Pigment composition & chlorophyllase activity in pigment pea (Cajanus indicus Sp reng) & gingelley(Seam un indicum L.) under NaCl salinity [J]. India J Exp Biol, 1981, 19: 768 - 770.
[200]李存桢,刘小京,杨艳敏等.盐胁迫对盐地碱蓬种子萌发及幼苗生长的影响[J].中国农学通报2005,25 (1):409- 413
[201]王冉,陈贵林,梁静等.盐胁迫对黑籽南瓜和白籽南瓜种子萌发特性的影响[J].河北农业大学学报, 2005,28(5): 42-44
[202]谢德意,王惠萍,王付欣.盐胁迫对棉花种子萌发及幼苗生长的影响[J].种子,2000,3:29-30
[203]Strogonov B P. Structure and function of plant cell in saline habitats[M]. New York: Halsted Press, 1973: 78 - 83.
[204]李晓燕,宋占平,董志贤.植物的盐胁迫生理[J].西北师范大学学报,2004,40(3):106-111
[205]杨秀玲.NaCl 胁迫对黄瓜种子萌发及幼苗生理特性的影响[D].甘肃农业大学硕士论文,2004, 1-10:46-47
[206]王建华,刘鸿先,戴若兰,王亚馥.超氧化物歧化酶(SOD)在植物逆境和衰老生理中的作用[J].植物生理学通讯,1989,(1):1-7
[207]郑丽锦.NaCl 胁迫下草莓生理生化特性研究[D].河北农业大学硕士论文,2003,18-21
[208]孙贵.盐胁迫对沙枣种子萌发及幼苗生长的效应[D].山东师范大学硕士论文,2000,5-6
[209] 肖波,廖尔华,胡开治等.植物生长物质诱导芦荟叶片愈伤组织效果的研究[J].中国农学通报, 2006.26(1):163- 165.
[210] Uppeandra Dhar1, Mitali Joshi. Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators[J]. Plant Cell Reports,2005,24(4):195-200.
[211]王声斌,黄自然,余让才等. 宽皮橘蕉柑的胚性愈伤组织诱导、悬浮细胞系建立及植株再生[J].华南农业大学学报(自然科学版), 2002,23(7):52-55
[2]周维燕.植物细胞工程原理与技术[M].中国农业大学出版社.北京,2001:39
[3]吴兴海.虎掌和猕猴桃组织培养的研究[D].陕西师范大学硕士论文,2003,1-10
[4]Steward FC, Mapes M0, Mears K. Growth and organized development of cultured cells, I. Organization in cultures from freely suspended cells[J]. Am J Bot, 1958, 45:705-708
[5].Zenk M H. Haploids in physiological and biochemical research[J]. In Haploids in Higher plants Advances Potential, 1974, (3):39-354
[6].裴翠绢,李洪杰,郭北海,等.冬小麦幼穗无性系变异分析[J].河北农业科学 1997,1(3):11-15
[7].周荣仁,杨燮荣,余叔文.利用组织培养研究植物耐盐机理与筛选耐盐突变体的进展[J].植物生理学通讯,1989,(5):11-19
[8]Nabors M W. In Randall DC(ed) Current Topics in plant biochemistry and physiology[J]. Uni of Missouri Columoia, 1983,.3:165.
[9]Skoog F, Miller CO. Chemical regulation of growth organ formation in plant tissue cultured in vitro[J]. Symp Soc Exp Biol, 1957, 11:118-131
[10]潘瑞炽.植物生理学(第 5 版) [M].高等教育出版社.北京,2004
[11]吴玮琳,叶文法,杨曼丽等.芳双酰胺衍生物合成及植物生长调节活性研究[J]. 武汉理工大学学报, 2004, 26(6):15-17
[12]Thorpe TA. Organogenesis in vitro: structural, physiological and biochemical aspects. In: Vasil IK (ed) Perspective in plant cell and tissue culture[M].A. Academic Press, New York,1980:71–105
[13]Yeung EC. Structural and developmental patterns in somatic embryogenesis[M]. In vitro embryogenesis in plants. Kluwer, Dordrecht, 1995: 205–247
[14]Y-H. Wang , P. L. Bhalla. Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br. [J] Plant Cell Rep, 2004, 22:408–414
[15]陈正华.木本植物组织培养及其应用[M].北京:高等教育出版社,1986
[16]Benelli C, Fabbri A, Grassi S, Lambardi M, Rugini E. Histology of somatic embryogenesis in mature tissue of olive (Olea europaea L.) [J]. J Hortic Sci Biotechnol, 2001,76:112–119
[17]Tisserat B. Embryogenesis, organogenesis and plant regeneration[M]. In: Dixon RA (ed) Plant cell culture: a practical approach. IRL Press, Eynsham, 1985:79–104
[18]傅立国,陈潭清,郎楷永,等主编.中国高等植物(第 6 卷)[M].山东:青岛出版社,2003:555-557
[19]吴国梁.不同激素对皱皮木瓜嫩茎段离体培养的影响[J].山西林业科技 1993,3:35-38
[20]范义莲,万益琴,刘志学.植物组织培养简报摘编[J],植物生理学通讯,2004,40(5):594
[21]程玉琴,韩振海,徐雪峰.苹果病毒及其脱毒检测技术研究进展[J],中国农学通报第 19 卷第 1 期 2003年 2 月:72-96
[22]洪艳华,殷广峰,张立军.百合脱毒及病毒检测技术研究进展[J].沈阳农业大学学报.2003,2006,34 (3):225- 227
[23]杨林栋.郭瑞锋.皇甫红芳.组织培养在马铃薯育种上的研究进展[J].中国马铃薯,2004,18(4):224-227
[24]伍成厚,潘一山,罗开梅等.蝴蝶兰未受精胚珠离体培养的研究[J].园艺学报2006,33(4):891～894
[25]金晓霞 张启翔.报春花属植物的育种研究进展[J],植物学通报.2005,22(6):738～74
[26]周朴华,何立珍,刘选明.组织培养中用秋水仙素诱发黄花菜同源四倍体的研究[J].中国农业科学, 1995 ,28(1):49~55
[27]何立珍,周朴华,刘选明,等.南荻同源四倍体的研究[J].遗传学报,1997,24(6):544~549
[28]吴伟刚,刘桂茹,杨学举..诱变与组织培养相结合在植物育种中的应用[J],中国农学通报.2005,21(11): 197-201
[29]张恩让,程智慧,周新民. 大蒜体细胞无性系的染色体[J]. 西北农林科技大学学报(自然科学版).2004, 32(9): 73-76
[30]Kirby, Edward. Plant Cell Cultures[J]. Bioscience; ProQuest Biology Journals,1989,39(9):645-646
[31]Ken J.Kasha. Biotechnology and world food supply. Genome; Aug 1999; 42(4):642-645
[32]孙振晓,马清温. 抗癌植物药及其植物组织培养研究进展[J].生物学通报,2000,35(8): 11-12
[33]MII M, OHASHIH.Plandet regeneration from protoplasm of kiwifruit,Actinidia chinensis planeh[J].Japan J Breed,1998,38:90-97.
[34]秦永华,张上隆,朱道圩.猕猴桃的组织培养和遗传转化研究进展[J].细胞生物学杂志, 2004,26(1):57-61
[35]桂耀林,徐延玉,母锡金.猕猴桃胚乳培养中的胚胎发生[J].武汉植物学研究,1988,6(4):395-397
[36]Nakano H.Plant differentiation in callus tissue induced from immature endosperm of Oryzasativa L Z[J]..Pflanzphysiol, 1975,76(5):444-449
[37]Progamme et al. Ⅷ Congress of the lnterational Society of Citriculture,Sun City Resort,South Africa, 1996,12:7
[38]Yang Xiaoling,Kitajima A,Hasegawa.ca1lus induction and Embyoid regeneration from the endosperm culture of ‘Tosa Buntan’ pummelo[J].Environment Control in Biology,2000,38(4):24l-246
[39]刘明,赵琦.高梁组织培养研究进展及关键技术[J].首都师范大学学报(自然科学版),2005,26(4): 60-63
[40]刘云国,王晓云.苹果种质资源玻璃化法超低温保存技术[J].山东农业大学学报(自然科学版),2002, 33(1):32-36
[41]中国科学院自然资源综合考察委员会.中国1:100 万土地资源图土地资源数据集[M],北京:中国人民大学出版社,1991
[42]叶笃正,陈泮勤主编. 中国的全球变化研究[M]. 北京:地质出版社,1992. 187-208
[43]赵可夫,李法曾主编.中国盐生植物[M].北京:科学出版社,1999.1,43-47
[44]FLOWERS, T. J.; TROKE, P. F. & YEO, A. R.: The mechanism of salt tolerance in halophytes. Annu. Rev. Plant Physiol. 1977,28:89-121
[45]MAAS, E. V. & NIEMAN, R. H.: Physiology of plant tolerance to salinity. In: JUNG, G. A. (ed.): Crop tolerance to suboptimal land conditions. Agron. Soc. America, Crop Sci. Soc. America and Soil Sci. Soc. America, Madison WI, 1978,277-299
[46]GORHAM, J.: Mechanism of salt tolerance of halophytes. In: CHOUKR-ALLAH, R., MALCOLM, C. V. & HAMDY, A. (eds.) : Halophytes and biosaline agriculture. Marcel Dekker, New York, 1995,207-223
[47]MUNNS, R.: Physiological processes limiting plant growth in saline soils: some dogmas and hypotheses[J]. Plant Cell Environ. 1993,16: 15-24.
[48]Muhammad Ashraf.:Some important physiological selection criteria for salt tolerance in plants[J]. Flora. Jena: 2004,199 (5):361-376
[49]许兴,毛桂莲,李树华等.NaCl 胁迫和外源 ABA 对枸杞愈伤组织膜脂过氧化及抗氧化酶活性的影响[J].西北植物学报,2003,23(5):745-749
[50] 陈利萍,徐春霞,李春顺.影响茎用芥菜愈伤组织诱导和植株再生的因素[J].植物生理学通讯,2005,41 (6): 758-760.
[51] Unnikrishnan, S. K.; Prakash, L.; Josekutty, P. C.; Bhatt, P. N.; Mehta, A. R. Effect of NaCl salinity on somatic embryo development in Sapindus trifoliatus L[J]. J. Exp. Bot. 1991,42: 401-406
[52] Beloualy, N; Boubarmont, J. NaCl-tolerant plants of Poncirus trigoliata regenerated from tolerant cell lines[J]. Theor. Appl. Genet. 1992,83:509-614
[53]Kumar, V.; Sharma, D. R. Isolation and characterization of sodium chloride-- resistant callus culture of Vigna radiata (L.) Wilczek var. radiata[J]. J. Exp. Bot. 1989 ,40:143-147
[54]Ericson. M. C. and Alfinito, S.H. Plant Physiol., 1984, 74:506
[55]Singh, N.K., Handa, A.K., HASEGAWA,p.m.et al., Plant Physiol,1985,79:126
[56] 陆 卫 , 贾 敬 芬 . 谷 子 胚 性 愈 伤 组 织 耐 盐 系 的 选 择 及 其 生 理 生 化 特 性 分 析 [J]. 作 物 学 报 1994,20(3):241-247
[57]张可炜,王先艳,王灵芝,等.利用细胞工程技术培育玉米耐盐自交系及单交种[J].山东农业科学,2001,6:15-17
[58]王鸣刚,陈亮,贾敬芬.耐盐突变体小麦后代耐盐稳定性分析研究[J]. 武汉植物学研究,2004, 22 (1):39-43
[59] 杜立群,李银心,李洪杰,等.在1/3海水培养基上筛选豆瓣菜耐盐变异体[J].植物学报1999,41 (6): 633-639
[60]M. Zacchini · A. Marotta · M. de Agazio.Tolerance to salt stress in maize callus lines with different polyamine content[J]. Plant Cell Reports 1997, 17: 119–122
[61]Y.MIKI,M.HASHIBA, S.HISAJIMA.Establishment of salt stress tolerate rice plants through step up NaCl treatment[J] in vitro.Biologia plantrum 2001,44(3):391-395
[62]岳玮,夏光敏,陈惠民,等.獐毛愈伤组织耐盐性研究及耐盐变异体的筛选[J].山东大学学报(自然科学版) 2000,35(3): 338-343
[63]郑霞,韦小敏,季良越,等.玉米体细胞抗盐突变体的筛选及耐盐性鉴定[J].河南农业大学学报 2004,38(2):139-143
[64]贺道耀,余叔文.水稻高脯氨酸变异系高脯氨酸含量和耐盐性的遗传性[J].植物生理学报 1997,23(4):357-362
[65]米海莉,许兴,李树华,等.春小麦耐盐突变体的筛选及耐盐性的研究[J].宁夏农林科技 2001,3:1-4
[66]祁翠兰,李志勇,刘秉信.红豆草耐盐突变体的研究[J].中国草地 1998,3:44-46
[67]郑文菊,王勋陵,贾敬芬.小麦耐盐系愈伤组织细胞的超微结构观察[J].西北植物学报 1999,19(1):81-84
[68]Smith M M,Hodson M J. Salt-induced ultrastructural damage to mitochondria in root tips of asalt-sensitive ecotype of Agrostisstolonifera[J]. J. Exp.Bot.,1982,33 (136) :886-895
[69]裴翠绢,李洪杰,郭北海,等.冬小麦幼穗无性系变异分析[J].河北农业科学 1997,1(3):11-15
[70]贾恢先,赵曼容,马莹.典型盐地植物细胞脂质过氧化伤害与质膜超微结构变化的研究[J].西北植物学报1994,14(6):1-5
[71]张恩让,程智慧.大蒜耐盐愈伤组织变异系的选择研究[J].西北植物学报 2003,23(9):1571-1576
[72]曾斌,罗淑萍,李疆.新疆野生巴旦杏的组织培养和植株再生[J].新疆农业大学学报,2006,29 (4) :27～31
[73]赵可夫,范海,宋杰,等.中国盐生植物的种类、类型、植被及其经济潜势.盐生植物利用与区域农业可持续发展[M].北京:气象出版社.2002
[74]彭峰,陈嫣嫣,郝日明等.彩色马蹄莲‘Parfait’不定芽诱导增殖培养条件的优化和筛选[J].植物资源与环境学报,2006, 15 (2) : 47 - 49
[75]陈金慧,施季森,诸葛强.杂交鹅掌楸的不定芽诱导及植株再生[J].植物生理学通讯, 2002,38(5):459
[76]李际红,谢会成,许建农等.穿山薯蓣不定芽的诱导与植株再生[J].山东农业大学学报(自然科学版) , 2005, 36 (1) : 82-85
[77]Samir C. Debnath.Clonal propagation of dwarf raspberry (Rubus pubescens Raf.) through in vitro axillary shoot proliferation[J].Plant Growth Regulation, 2004,43:179–186
[78]李林,李科友,唐德瑞.白皮松离体胚培养与不定芽的诱导[J].西北林学院学报,2004, 19 (2) : 61- 63
[79]陆瑞菊, 黄剑华, 王亦菲等.三倍体毛白杨快繁技术研究[J].中国农学通报,2004,19(2): 80-82
[80]武宗信,解红娥,冯文龙等.地黄脱毒技术研究[J].中药材,2002,25(6):383-385
[81]宋建英,叶建仁.“马可”百合组培技术研究[J]. 南京林业大学学报(自然科学版) ,2006 ,30(7):73-76
[82]周瑞金,刘孟军.枣离体叶片高效再生植株的研究[J].园艺学报,2006, 33 (3) : 625～628,
[83]王鸿,马锋旺,郝,燕等.山杏下胚轴再生植株的研究[J].西北农林科技大学学报(自然科学版),2005,33 (5):137-130
[84]王光萍,丁雨龙,黄敏仁等.观赏竹的试管快繁研究[J].林业科学,2005,41(5):51-55
[85] 陈 菊 , 陈 国 惠 . 何 首 乌 生 根 培 养 因 子 的 优 选 研 究 [J]. 西 南 农 业 大 学 学 报 ( 自 然 科 学 版 ) 2006,28(5):576-578
[86]曾炳山,刘英,许煌灿等.长嘴黄藤离体快繁研究[J].福建林学院学报,2002, 22 (2) : 169-171
[87]张超,陈奉玲,汤兴毫.木瓜的本草考证[J].中草药,1999,30(12):943.
[88]吴延廷,张俊.中药木瓜的基源和性状鉴定[J].华西医科大学学报,1996,27(4):404- 408.
[89]洪永福,郭学敏.三种木瓜的乙醚提取部位的气相色谱-质谱分析[J].第二军医大学学报,2000, 21(8):749-752.
[90]潘瑞炽.植物组织培养[M].北京:科学出版社,2003.
[91]刘先勇,袁长迎,段宝福等.SPSS10.0统计分析软件与应用[M].北京:国防工业出版社,2002.
[92]符文英,杜道林,符木均.海南粗榧愈伤组织的诱导和培养[J].植物生理学通讯,2004 ,40(1):33-36
[93]许忠民,张恩慧,欧承刚,等.不同因素对甘蓝花药愈伤组织诱导的影响[J].西北农林科技大学学报(自然科学版) . 2006,34(9): 65-68
[94]陈永勤,朱蔚华,吴蕴祺等.云南红豆杉细胞培养和紫杉醇生产[J].湖北大学学报(自然科学版),2001,23(4):366-369
[95]M. K. Kim ,H. E. Sommer,B. C. Bongarten,S. A. Merkle.High-frequency induction of adventitious shoots from hypocotyl segments of Liquidambar styraciflua L. by thidiazuron[J]. Plant Cell Reports,1997,16: 536–540
[96]魏小勇.铁皮石斛原球茎悬浮培养研究[J].现代中药研究与实践,2004,18(4):7-11
[97]王镜岩,朱圣庚,徐长法.生物化学[M].高等教育出版社,北京,2002
[98]刘贵周,谢世清,赵庆云登.优质魔芋组培快繁技术研究[J].云南农业大学学报,2005,20(6):795-799
[99]李玉平,王永宏,张强等.大花金挖耳无菌外植体的获得及愈伤组织诱导的初步研究[J].西北农林科技大学学报(自然科学版),2006,34(10):171-176
[100]盛长忠,王淑芳,王宁宁等.红豆杉愈伤组织培养中褐变现象的初探[J].南开大学学报(自然科学),2001,34 (4): 120-122
[101]邱璐,王波,王志和等.史密斯桉愈伤组织的诱导及分化[J].东北林业大学学报, 2006,34(4):18-21
[102]李琳,叶春.影响库拉索芦荟(Aloevera L.)愈伤组织诱导的因素[J].四川大学学报(自然科学版), 2003,40(5): 963-965
[103]钱永强,孙振元,李云等.野牛草成熟胚植株再生及其影响因素研究[J].草业科学,2005,22(2):101-106
[104]陈芳清,丘安机,徐祥浩.药用植物红芽大戟的组织培养[J].广西植物, 1997,17(2):149-151
[105]王雅英,杨忠耿,陈汉鑫等.新台糖25号甘蔗愈伤组织诱导试验[J].亚热带植物科学,2003,32(4):30-32.
[106]袁鹰,刘德璞,郑培和等.钢东北玉米自交系胚性愈伤组织的诱导[J].玉米科学,2001,9(1):37-38
[107]袁红霞,王金虎,陈德燕等.何首乌块根愈伤组织培养[J].苏州大学学报(自然科学版) ,2005,21(13): 86-88
[108]王建华,王义,孙国伟等.人参组织培养的多因子正交试验研究[J].吉林农业大学学报, 2006,28(16):468-451
[109]朱靖杰,王宇光,雷禄旺等.皇帝蕉薄片外植体愈伤组织的诱导及植株再生[J].果树学报,2006, 23(1):111-114
[110]陈芳清,丘安机,徐祥浩.药用植物红芽大戟的组织培养[J].广西植物, 1997,17(2):149-151
[111]王雅英,杨忠耿,陈汉鑫等.新台糖25号甘蔗愈伤组织诱导试验[J].亚热带植物科学,2003,32(4):30-32
[112]洪岚,叶万辉,沈浩等.薇甘菊组织培养及体细胞胚胎发生的研究[J].浙江大学学报(农业与生命科学版),2005,31 (5) : 572-578
[113]乔琦,肖娅苹,王喆之.外源激素对防风体细胞胚发生和发育的影响[J].西北大学学报(自然科学版),2005,35(3): 316-320.
[114]陈利萍,徐春霞,李春顺.影响茎用芥菜愈伤组织诱导和植株再生的因素[J].植物生理学通讯,2005,41(6):758-760.
[115]陆美莲,许新萍,周厚高等.均匀正交设计在百合组织培养中的应用[J].西南农业大学学报(自然科学版).2004,26 (6):699-703.
[116]陈火英,张建华,俞俊棠等.番茄离体培养过程中器官发生的细胞组织学观察[J].武汉植物学研究,2001,19(2): 91-95
[117]皮伟.几个因素对草地早熟禾种子愈伤组织诱导的影响[J].西南农业大学学报(自然科学版),2006,28(5): 747-749
[118]韩珊,石大兴,王米力等.红叶乌桕茎段离体培养的研究[J].四川农业大学学报,2006,24(3):301-302
[119]白文苑,沈慧敏.苍耳愈伤组织诱导及继代培养研究[J].甘肃农业大学学报,2006,41(1):65-68
[120]刘航空,韩明玉,禹婷等.影响油桃叶片产生胚性愈伤组织的因素[J].果树学报,2006, 23( 3):370-374
[121]李毅,邸利,洪涛等. 箭胡毛杨愈伤组织诱导、保存与再分化[J].西北植物学报,2002,22(3): 656-600
[122]姜蕾,兰天维,黎扬辉等.影响红掌愈伤组织诱导、增殖和芽分化的因素[J].种子, 2006,25(11): 26-30
[123]马均,马明东,周宇.爝曼地亚红豆杉愈伤组织诱导试验[J].林业科技,2006,31(1):12-14
[124]刘金英,徐有明,李双来等.佛手山药组织培养的研究[J].植物研究,2006,26(3):323-328
[125]韩清霞,沈火林,朱鑫等.芹菜胚性愈伤的诱导及高频植株再生体系的建立[J].中国蔬菜,2006 (11) : 6～9
[126]龚伟,胡庭兴,宫渊波等.光叶子花茎段愈伤组织的诱导及其植株再生的研究[J].园艺学报,2005,32 (6):1125-1128
[127]肖荷霞,王瑛,高峰等.外植体及激素对SANDITI紫花苜蓿愈伤组织诱导和分化的影响[J].河北农业大学学报, 2003,26(4):47-52
[128]毛堂芬,黄先群.少花桂愈伤组织诱导的初步研究[J].种子,2005,24(8):75-76
[129]彭晓莉,王蒂,张金文等.激素诱导下不同培养方式对马铃薯微型薯的诱导效应[J].甘肃农业大学学报,2006, 41(1):16-19
[130]闫桂琴,田丽宏,杨利艳.濒危植物翅果油树愈伤组织诱导中褐变问题的研究[J].西北植物学报,2004, 24(8):1384-1389
[131]黄远新,何凤发,张盛林.魔芋组织培养与快繁技术研究[J].西南农业大学学报(自然科学版),2003,25(4): 309-312
[132]高蓉,赵博光.防止黑松外植体及其愈伤组织褐变的方法[J].南京林业大学学, 2001,25(5):75-77
[133]付成华,沈宝仙.I-72杨再生体系的建立[J].华中农业大学学报,2006,25(5):564-567
[134]刘国诠.生物工程下游技术[M].化学工业出版社,北京,2003
[135]王志强,王惠林,廖新福等.新疆哈密瓜子叶不定芽诱导与植株再生[J].新疆农业大学学报,2005,28 (3):79-81
[136]金宝燕,苏华,任华中.影响黄瓜直接不定芽诱导的品种等因素研究[J].中国农学通报, 2006,22(6):45-48
[137]Chalupa V, Large scale micropropagation of Quercus roburL. using adenine-type cytokinins and thidiazuron to stimulateshoot proliferation[J]. Bio Plant,1988,30:414–421.
[138]Lu C-Y. The use of thidiazuron in tissue culture[J]. In Vitro Cell Dev Biol,1993,29:92–96
[139]Van Nieuwkerk JP, Zimmerman RH, Fordham I (1986) Thidiazuron stimulation of apple shoot proliferation[J] in vitro. HortScience 21:516–518
[140]Yusnita S, Geneve RL, Kester ST, Micropropagation of white flowering eastern redbud (Cercis canadensis var ‘alba’ L.) [J]. J Environ Hortic ,1990, 8:177–179
[141]Murat ?zgen, Sebahattin ?zcan1_, Cafer S. Sevimay, Cengiz Sancak & Mustafa Y?ld?z High frequency adventitious shoot regeneration in sainfoin[J].Plant Cell, Tissue and Organ Culture,1998, 52: 205–208
[142]Ben A. Bergmann,Heung-Kyu Moon.In vitro adventitious shoot production in Paulownia[J]. Plant Cell Reports,1997, 16:315-319
[143]Wei Tang, Fan Ouyang.Plant regeneration via organogenesis from six families of loblolly pine[J]. PlantCell, Tissue and Organ Culture,1999,58:223–226,.
[144]张国裕,程智慧,李娟等.银条不定芽诱导及高效增殖体系的建立[J].西北农林科技大学学报(自然科学版), 2004,32 (2):49-52
[145]师校欣,杜国强,马俊莲等.磨盘柿离体叶片愈伤组织发生及不定芽诱导[J].果树学报2004, 21(4): 376-378
[146]Ting-Yu Chen, Jen-Tsung Chen ,Wei-Chin Chang.Plant regeneration through direct shoot bud formation from leaf culturesof Paphiopedilum orchids[J].Plant Cell, Tissue and Organ Culture,2004, 76:11–15
[147]田宏现,曾艳玲,谭晓风等. 米良一号猕猴桃的组织培养研究[J].经济林研究,2005,23 (1):7-9
[148]Chun-Lai Zhang; Dong-Fang Chen; Malcolm C Elliott et al.Efficient procedures for callus induction and adventitious shoot organogenesis in sugar beet (beta vulgaris l.) breeding lines[J]. In Vitro Cellular & Developmental Biology,2004, 40(5):475-481
[149]汤浩茹,刘翠琼,罗娅.满园香梨叶片不定芽的诱导[J].果树学报,2005,22(6):706-708
[150]Y.E. Choi,D.-C. Yang,E.-S. Yoon etal.Plant regeneration via adventitious bud formation from cotyledon explants of Panax ginseng C.A. Meyer[J] Plant Cell Reports,1998, 17: 731–736
[151]Eunju Cheong,Margaret R Pooler.Micropropagation of Chinese redbud (cercis yunnanensis) through axillary bud breaking and induction of adventitious shoots from leaf pieces[J].In vitro cellular & developmental biology. 2003,39(5):455-458
[152]A.K. Kobayashi , J.C. Bespalhok , L.F.P. Pereira,L.G.E. Vieira1, Plant regeneration of sweet orange (Citrus sinensis) from thin sections ofmature stem segments[J].Plant Cell, Tissue and Organ Culture ,2003 74: 99–102
[153]Cheng Zhu,Zhen Chen.Role of polyamines in adventitious shoot morphogenesis from cotyledons of cucumber[J]. In vitro Plant Cell Tiss Organ Cult,2005, 81:45–53
[154]G. Liu,M. Bao.Adventitious shoot regeneration from in vitro cultured leaves of London plane tree (Platanus acerifolia Willd.) [J].Plant Cell Rep,2003,21:640–644
[155]LARKIN P J , SCOWCROFTW R. Somaclonal variation-anovel source of variablity from cell cultures for plant improvement [J]. Theor. App. Genet, 1981, 60: 117-124.
[156]SALINML Toxic oxygen species and protective systems of the chloroplast[J]. Physiol.Plant, 1988,72:681-689.
[157]魏玉清,许兴,王璞.土壤盐胁迫下宁夏枸杞的生理反应[J].中国农学通报,2009,21(9)213-217
[158]刘凤荣,陈火英,刘杨等.盐胁迫下不同基因型番茄可溶性物质含量的变化[J].植物生理与分子生物学报,2004 ,30 (1):99-104
[159]赵可夫.植物抗盐生理[M ].北京:中国科学技术出版社, 1993: 24 - 27, 230, 231.
[160]Rosario Vera-Estrella1, Bronwyn J. Barkla1, Hans J. Bohnert2, Omar Pantoja1 Salt stress in Mesembryanthemum crystallinum L. cell suspensions activates adaptive mechanisms similar to those observed in the whole plant[J], Planta, 1999, 207: 426-435
[161]赵素然,梁宇,李德森等.毛白杨愈伤组织悬浮细胞耐盐性研究[J].广西植物,2000,20 (4) : 351-355
[162] 杨 素 欣 , 王 振 镒 . 盐 胁 迫 下 小 麦 愈 伤 组 织 生 理 生 化 特 性 的 变 化 [J]. 西 北 农 业 大 学 学报.1999,27(2):48-51
[163]齐志广,杨献光,徐涛等.盐胁迫对不同基因型小麦成熟胚愈伤组织生长的影响[J].2004,28(1):河北师范大学学报(自然科学版):68-71
[164]武维华.植物生理学[M].科学出版社,北京,2003
[165]张美云, 钱吉, 钟扬等.野生大豆若干耐盐生理指标的研究[J].复旦学报(自然科学版),2002,41(6): 669-673
[166]Ireneusz S lesak, Marta Libik & Zbigniew Miszalski. Superoxide dismutase activity in callus from the C-CAM intermediate plant Mesembryanthemum crystallinum[J]. Plant Cell, Tissue and Organ Culture ,2003,75: 49–55,
[167]Barbara Karpinska, Marlene Karlsson, Helga Schinkel et al.A Novel Superoxide Dismutase with a High Isoelectric Point in Higher Plants[J]. Plant Physiology, 2001, 126:1668–1677
[168]SONG Fu-nan, YANG Chuan-ping, LIU Xue-mei, LI Gong-bin.Effect of salt stress on activity of superoxide dismutase (SOD) in Ulmus pumila L[J]. Journal of Forestry Research, 2006,17(1): 13–16
[169]宋福南,杨传平,刘雪梅等.盐胁迫对怪柳超氧化物歧化酶活性的影响[J].东北林业大学学报,2006,34(5):54-56
[170]魏爱丽,白桦,陈云昭等.盐胁迫下大豆初生叶愈伤组织SOD活性及其同工酶变化的研究[J].大豆科学,1999,18(1): 85-88
[171]贺岩,李志岗,李新鹏,赵娟.盐胁迫条件下两种基因型小麦生长及保护酶活性的反应[J].山西农业大学学报, 2005,25 (1):42-44
[172]Yardena Gueta-Dahan, Zohara Yaniv, Barbara A. Zilinskas, Gozal Ben-Hayyim. Salt and oxidative stress: similar and specie responses and their relation to salt tolerance in Citrus[J].Planta ,1997,203: 460-469
[173]谭大海,沙伟,张莹莹.芦苇盐胁迫下渗透调节物质含量变化研究[J].齐齐哈尔大学学报,2006,22(2):84-86
[174]卢静君,多立安,刘祥君.盐胁迫下两草种SOD和POD及脯氨酸动态研究[J].植物研究, 2004,24(1): 115-119
[175]刘会超,孙振元,彭镇华.NaCl胁迫对五叶地锦生长及某些生理特性的影响[J].林业科学,2004,40(6):63 -67
[176]万劲,石雷,张金政等.盐胁迫对鸢尾叶片生理指标的影响[J].南京林业大学学报(自然科学版)2006,30 (1):57-60
[177]Valentine RC.Californian Agriculture,1984,38(10):36
[178]李卫欣,陈贵林,赵利等.NaCI 胁迫下不同南瓜幼苗耐盐性研究[J].植物遗传资源学报2006 ,7 (2): 192-196
[179]费伟,陈火英,曹忠等.盐胁迫对番茄幼苗生理特性的影响[J].上海交通大学学报(农业科学版第), 2005,23(1)-5-11
[180]Sangita Basu, Gaurab Gangopadhyay,B.B. Mukherjee. Salt tolerance in rice in vitro: Implication of accumulation of Na+, K+ and praline[J].Plant Cell, Tissue and Organ Culture 2002, 69: 55–64
[181]裘丽珍,黄有军,黄坚钦等.不同耐盐性植物在盐胁迫下的生长与生理特性比较研究[J].浙江大学学报(农业与生命科学版), 2006,32 (4) : 420-427
[182]李国雷 ,孙明高,夏阳等.NaCl 胁迫下黄栌、紫荆的部分生理生化反应动态变化规律的研究[J].山东农业大学学报(自然科学版) , 2004 , 35 (2) : 173-176
[183]吴能表,叶腾丰,,王小佳.NaCl胁迫对豌豆幼苗生理生化指标的动态影响[J].西南农业大学学报(自然科学版),2006, 28(1): 37-40
[184]王征宏,杨起,张亚冰.盐胁迫下紫花苜蓿种子的萌发特性[J].河南科技大学学报(自然科学),2006,27(1): 67-80
[185]郇树乾,刘国道,张绪元等.NaCl胁迫对刚果臂形草种子萌发及幼苗生理效应的研究[J].中国草地, 2004,26(6) : 45 - 49.
[185]尹增芳,何祯祥,王丽霞,钦佩.NaCl 胁迫下海滨锦葵种子萌发和幼苗生长过程的生理特性变化[J].植物资源与环境,2006(1):14-17
[186]Romero Aranda R, Soria T,Cuartero J.Tomato plant-water uptake and plant-water relationships under saline growth conditions [J]. Plant Science, 2001, 160: 265 - 272.
[187]Wang Y, NilN. Changes in chlorophyll, ribulose biphosphate carboxylase - oxygenase, glycine betaine content, photosynthesis and transpiration in Amaranthus tricolor leaves during salt stress[J]. J Hortic SciBiotechnol, 2000, 75: 623 - 627.
[188]於丙军,李锁娜,刘友良.大豆苗期盐害离子效应的比较[J].南京农业大学学报, 2002, 25 (1):5 - 9.
[189]王仁雷,华春,刘友良.盐胁迫对水稻光合特性的影响[J].南京农业大学学报, 2002, 25 (4) : 11 - 14.
[190]董晓霞,赵树慧,孔令安,等.苇状羊茅盐胁迫下生理效应的研究[J].草业科学, 1998,15 (5) : 10-13.
[191]董晓霞,郭洪海,孔令安.杂交酸模耐盐性鉴定的生理指标筛选[J].山东农业科学, 1999 (6) : 22 - 24
[192]刁丰秋,章文华,刘友良.盐胁迫对大麦叶片内囊体膜组成和功能的影响[J].植物生理学报,1997,23(2):105-110
[193]夏阳,孙明高,李国雷等.盐胁迫对四园林绿化树种叶片中叶绿素含量动态变化的影响[J].山东农业大学学报(自然科学版),2005,36 (1):30-34
[194]朱进,别之龙,李娅娜等.盐胁迫对不同基因型黄瓜幼苗生长和光合作用的影响[J].沈阳农业大学学报. 2006- 06.37(3):476- 478
[195]Santos C, Caldeira G. Comparative responses of Helianthus annuus plants and calli exposed to NaCl:. Growth rate and osmotic regulation in intact plants and calli[J]. J Plant Physiol, 1999, 155: 769 - 777.
[196]Santos C, Azevedo H, Caldeira G. In situ and in vitro senescence induced by KCl stress: nutritional imbalance, lip id peroxidation and antioxidantmetabolism[J]. J Exp Botany, 2001: 52: 351 - 360.
[197]Santos C. Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves[J]. Scientia Horticultures, 2004, 103: 93 - 99.
[198]刘家尧,衣艳君,张其德.盐胁迫对不同抗盐性小麦叶片荧光诱导动力学的影响[J].植物学通报,1998, 15 (2):46 - 49.
[199]Rao G G, Rao G R. Pigment composition & chlorophyllase activity in pigment pea (Cajanus indicus Sp reng) & gingelley(Seam un indicum L.) under NaCl salinity [J]. India J Exp Biol, 1981, 19: 768 - 770.
[200]李存桢,刘小京,杨艳敏等.盐胁迫对盐地碱蓬种子萌发及幼苗生长的影响[J].中国农学通报2005,25 (1):409- 413
[201]王冉,陈贵林,梁静等.盐胁迫对黑籽南瓜和白籽南瓜种子萌发特性的影响[J].河北农业大学学报, 2005,28(5): 42-44
[202]谢德意,王惠萍,王付欣.盐胁迫对棉花种子萌发及幼苗生长的影响[J].种子,2000,3:29-30
[203]Strogonov B P. Structure and function of plant cell in saline habitats[M]. New York: Halsted Press, 1973: 78 - 83.
[204]李晓燕,宋占平,董志贤.植物的盐胁迫生理[J].西北师范大学学报,2004,40(3):106-111
[205]杨秀玲.NaCl 胁迫对黄瓜种子萌发及幼苗生理特性的影响[D].甘肃农业大学硕士论文,2004, 1-10:46-47
[206]王建华,刘鸿先,戴若兰,王亚馥.超氧化物歧化酶(SOD)在植物逆境和衰老生理中的作用[J].植物生理学通讯,1989,(1):1-7
[207]郑丽锦.NaCl 胁迫下草莓生理生化特性研究[D].河北农业大学硕士论文,2003,18-21
[208]孙贵.盐胁迫对沙枣种子萌发及幼苗生长的效应[D].山东师范大学硕士论文,2000,5-6
[209] 肖波,廖尔华,胡开治等.植物生长物质诱导芦荟叶片愈伤组织效果的研究[J].中国农学通报, 2006.26(1):163- 165.
[210] Uppeandra Dhar1, Mitali Joshi. Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators[J]. Plant Cell Reports,2005,24(4):195-200.
[211]王声斌,黄自然,余让才等. 宽皮橘蕉柑的胚性愈伤组织诱导、悬浮细胞系建立及植株再生[J].华南农业大学学报(自然科学版), 2002,23(7):52-55