有机小分子污染物与生物大分子相互作用的研究
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摘要
近年来，由于有机污染物的大量出现以及人们环保意识的提高，各类有机污染毒物性质以及其对人体造成的危害越来越受到人们的关注。有机污染毒物的管理与有害废弃物的处理是和人们息息相关的课题，为了更好的控制各种有害有机物，了解有害物质的生命周期及其与生物大分子之间的反应机理是极为重要的。此外小分子与生物大分子的相互作用研究，对发现新药物和在基因水平上疾病的治疗，尤其是药物分子的作用机理也具有非常重要的意义，因此一直是人们关注和研究的课题之一。因此，本文选定了一些典型的有机小分子，以近年来新发展起来的共振光散射(resonance light scattering，RLS)技术为基本手段，研究了小分子与蛋白质、核酸之间的相互作用。
主要研究内容如下：
1．依思明蓝与牛血清白蛋白相互作用
在pH 3.78水溶液中，血清白蛋白可与染料探针依思明蓝通过疏水作用结合并产生以367.4nm为特征峰的共振光散射(RLS)增强信号。在该特征波长下测定的增强共振光散射强度(△I_(RLS))与血清白蛋白浓度在一定范围内具有线性关系。据此建立了痕量血清蛋白的共振光散射分析法。当依思明蓝的浓度为1.5×10~(-5)mol／L时，牛血清白蛋白、人血清白蛋白以及IgG的检测限分别可达2.34ng／mL、6.86ng／mL和10.9ng／mL。基于共振光散射数据及Scatchard图，获得依思明蓝与牛血清白蛋白和人血清白蛋白的结合常数(K)以及结合位点数(n)分别为2.90×10~5M~(-1)，2.9和2.96×10~5M~(-1)，0.35。
2．阴离子表面活性剂与白蛋白与球蛋白相互作用的散射偏振研究
在酸性缓冲溶液中，十二烷基硫酸钠(SDS)可以与人血清白蛋白(HSA)以及免疫球
Recently, with the appearance of organic waste and the improvement of people's environmental consciousness, more and more people are interesting in the nature source and chemistry of all kinds of hazardous organic wastes and the harm approach to mankind. Hazardous organic materials and waste management is a topic that touches all lives. Every day people encounter hazardous materials (chemicals) or hazardous waste in their home, school or place of business. In order to properly manage hazardous organic waste, it is important to consider the entire life cycle, which include when the waste was a useful chemical or hazardous material and the reaction mechanism between organic small molecules and biomacromolecules. Moreover, the study of reaction mechanism between organic small molecules and biomacromolecules has very import meaning for finding new drugs、 curing diseases form gene levels and studying the reaction mechanism, so it is always scholar's interesting and attentional subject. So we select some typical organic small molecules and study the reaction mechanism between organic small molecules and proteins、 DNA based on the newly developmental resonance light scattering technique. The following are the main investigative systems:
1. The Interaction of Isamin Blue with BSA
At pH 3.78, the static interaction of IB with BSA occurs and results in greatly enhanced resonance light scattering (RLS) signals characterized by a peak at 367.4 nm. It was found that the enhanced RLS intensity (ΔI_(RLS)) at 367.4 nm is proportional to the concentration of BSA, and thus a new method for trace BSA analysis could be established accordingly. The limit of determination (3σ) is 2.34 ng/mL when 1.5 × 10
主要研究内容如下：
1．依思明蓝与牛血清白蛋白相互作用
在pH 3.78水溶液中，血清白蛋白可与染料探针依思明蓝通过疏水作用结合并产生以367.4nm为特征峰的共振光散射(RLS)增强信号。在该特征波长下测定的增强共振光散射强度(△I_(RLS))与血清白蛋白浓度在一定范围内具有线性关系。据此建立了痕量血清蛋白的共振光散射分析法。当依思明蓝的浓度为1.5×10~(-5)mol／L时，牛血清白蛋白、人血清白蛋白以及IgG的检测限分别可达2.34ng／mL、6.86ng／mL和10.9ng／mL。基于共振光散射数据及Scatchard图，获得依思明蓝与牛血清白蛋白和人血清白蛋白的结合常数(K)以及结合位点数(n)分别为2.90×10~5M~(-1)，2.9和2.96×10~5M~(-1)，0.35。
2．阴离子表面活性剂与白蛋白与球蛋白相互作用的散射偏振研究
在酸性缓冲溶液中，十二烷基硫酸钠(SDS)可以与人血清白蛋白(HSA)以及免疫球
Recently, with the appearance of organic waste and the improvement of people's environmental consciousness, more and more people are interesting in the nature source and chemistry of all kinds of hazardous organic wastes and the harm approach to mankind. Hazardous organic materials and waste management is a topic that touches all lives. Every day people encounter hazardous materials (chemicals) or hazardous waste in their home, school or place of business. In order to properly manage hazardous organic waste, it is important to consider the entire life cycle, which include when the waste was a useful chemical or hazardous material and the reaction mechanism between organic small molecules and biomacromolecules. Moreover, the study of reaction mechanism between organic small molecules and biomacromolecules has very import meaning for finding new drugs、 curing diseases form gene levels and studying the reaction mechanism, so it is always scholar's interesting and attentional subject. So we select some typical organic small molecules and study the reaction mechanism between organic small molecules and proteins、 DNA based on the newly developmental resonance light scattering technique. The following are the main investigative systems:
1. The Interaction of Isamin Blue with BSA
At pH 3.78, the static interaction of IB with BSA occurs and results in greatly enhanced resonance light scattering (RLS) signals characterized by a peak at 367.4 nm. It was found that the enhanced RLS intensity (ΔI_(RLS)) at 367.4 nm is proportional to the concentration of BSA, and thus a new method for trace BSA analysis could be established accordingly. The limit of determination (3σ) is 2.34 ng/mL when 1.5 × 10
引文
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