益气温阳复方对糖尿病心肌病大鼠血清中NO及SOD含量影响的实验研究
摘要
目的:本实验通过观察中药益气温阳复方对自发性非肥胖Ⅱ型糖尿病大鼠(GK大鼠)进行干预,测定GK大鼠血清中一氧化氮(NO)、超氧化物岐化酶(SOD)含量的变化,以探讨益气温阳复方防治糖尿病心肌病作用机制及其作用靶点,为中医药治疗糖尿病心肌病开拓新思路、提供客观、真实、科学的实验数据。
材料与方法:取健康体重300～350克的雄性GK大鼠30只及普通级雄性大鼠10只;普通级大鼠作为正常对照组(n=10),喂以普通饲料8周;GK大鼠(n=30)喂以高脂饲料4周后,随机分为三组,即模型组(n=10)、中药组(益气温阳复方)(n=10)、西药组(雷米普利组)(n=10)。模型组、中药组、西药组继续喂以高脂饲料;给药4周后实验结束。于第2、4、8周,检测各组大鼠血糖变化情况,第8周末检测各组大鼠血脂和心功能的变化,处死各组大鼠,采用硝酸还原酶法及黄嘌呤氧化酶法测定检测大鼠血清中NO、SOD的含量表达情况。
结果:
1.与正常组相比,模型组大鼠的血糖、心功能、血脂及血清中NO含量明显升高,血清中SOD含量明显降低,具有统计学意义。
2.与模型组相比,各给药组大鼠血糖、心功能、血脂及血清中NO含量明显降低,血清中SOD含量明显升高,具有统计学意义。
3.各给药组的大鼠血糖、心功能、血脂、血清中NO、SOD含量的组间比较,无明显统计学差异。
结论:
1.用自发性非肥胖Ⅱ型糖尿病大鼠(GK大鼠)配以高脂饲料的喂养的方法建立的实验性大鼠DCM模型可以加重其心肌组织损伤,与人类DCM发生发展相近似。
2.益气温阳复方能够抑制自发性非肥胖Ⅱ型糖尿病大鼠(GK大鼠)血清中NO的水平含量并降低大鼠血糖、血脂水平,并能提高血清中SOD含量的表达,提示益气温阳复方可防治DCM。
3. DCM中医病因为消渴久延,脏腑功能失调,阴阳气血受损,导致血脉通行受阻,日久心脉痹阻发为本病。其病机关键为气虚、阳衰,故以益气、温阳为治疗本病的基本治则。
Purpose:To investigate and explore the effect of the qi-supplementing and Yang-warming compound recipe in GK rats, measuring the changes of nitric oxide( NO)、and superoxide dismutase (SOD)content in serum of the rats (GK rats), to explore action mechanism and its targets for TCM therapy for diabetes cardiomyopathy develop new ideas and provide objective, realistic and scientific experimental data.
Material and method:10 male rats were divided into normal group,as a normal tolerances rats (n = 10 ),fed with normal diet for eight weeks ;and 30 male GK rats were feed with high fat diet and then were randomly divided into three groups:the model group(n = 10 ), chinese medicine,the temperature of the group and its(n = 10),western medicine group ,ramipril(n = 10). Model groups, traditional chinese medicine, and western medicine group continue to feed with high fat feed .Experiment end in drug intervention 4 weeks later.
Results:
1. Compared with the normal group, blood sugar, cardiac function, blood fat and NO content in serum of the rats in the model group are significantly raised, SOD content in serum is significantly lowered, there are statistical significances.
2. Compared with the model group, blood sugar, cardiac function, blood fat and NO content in serum of the rats in the administration groups are significantly lowered, and SOD content in serum is significantly raised.
3. There are no statistically significant difference in accordance with the comparison of blood sugar, cardiac function, blood fat and NO, SOD contents in serum of the rats among the administration groups.
Conclusion:
1. The experimental rat DCM (dilated cardiomyopathy) model, established based on the method for feeding high fat diet to rats (GK rats) with spontaneous non-obesity Type 2 diabetes mellitus, could aggravate their myocardial tissue injury, which is similar to the onset and development of human DCM.
2. The qi-supplementing and Yang-warming compound can suppress NO content in serum of the rats (GK rats) with spontaneous non-obesity Type 2 diabetes mellitus and lower the level of blood sugar and blood fat of the rats, and can also enhance the expression of SOD content in serum. Prompt: the qi-supplementing and Yang-warming compound can used for the prevention and treatment of DCM.
3. The DCM traditional Chinese medicine disease could cause blood vessel blockage and heart vessel blockage stasis after a long time because of long-term delay of thirst relieving, viscera dysfunction and yin and yang qi-blood injury, further turning into this disease. The key of its pathogenesis lies in deficiency of qi and yang, so the fundamental rule for the treatment of this disease shall fall upon qi supplementing and yang warming.
材料与方法:取健康体重300～350克的雄性GK大鼠30只及普通级雄性大鼠10只;普通级大鼠作为正常对照组(n=10),喂以普通饲料8周;GK大鼠(n=30)喂以高脂饲料4周后,随机分为三组,即模型组(n=10)、中药组(益气温阳复方)(n=10)、西药组(雷米普利组)(n=10)。模型组、中药组、西药组继续喂以高脂饲料;给药4周后实验结束。于第2、4、8周,检测各组大鼠血糖变化情况,第8周末检测各组大鼠血脂和心功能的变化,处死各组大鼠,采用硝酸还原酶法及黄嘌呤氧化酶法测定检测大鼠血清中NO、SOD的含量表达情况。
结果:
1.与正常组相比,模型组大鼠的血糖、心功能、血脂及血清中NO含量明显升高,血清中SOD含量明显降低,具有统计学意义。
2.与模型组相比,各给药组大鼠血糖、心功能、血脂及血清中NO含量明显降低,血清中SOD含量明显升高,具有统计学意义。
3.各给药组的大鼠血糖、心功能、血脂、血清中NO、SOD含量的组间比较,无明显统计学差异。
结论:
1.用自发性非肥胖Ⅱ型糖尿病大鼠(GK大鼠)配以高脂饲料的喂养的方法建立的实验性大鼠DCM模型可以加重其心肌组织损伤,与人类DCM发生发展相近似。
2.益气温阳复方能够抑制自发性非肥胖Ⅱ型糖尿病大鼠(GK大鼠)血清中NO的水平含量并降低大鼠血糖、血脂水平,并能提高血清中SOD含量的表达,提示益气温阳复方可防治DCM。
3. DCM中医病因为消渴久延,脏腑功能失调,阴阳气血受损,导致血脉通行受阻,日久心脉痹阻发为本病。其病机关键为气虚、阳衰,故以益气、温阳为治疗本病的基本治则。
Purpose:To investigate and explore the effect of the qi-supplementing and Yang-warming compound recipe in GK rats, measuring the changes of nitric oxide( NO)、and superoxide dismutase (SOD)content in serum of the rats (GK rats), to explore action mechanism and its targets for TCM therapy for diabetes cardiomyopathy develop new ideas and provide objective, realistic and scientific experimental data.
Material and method:10 male rats were divided into normal group,as a normal tolerances rats (n = 10 ),fed with normal diet for eight weeks ;and 30 male GK rats were feed with high fat diet and then were randomly divided into three groups:the model group(n = 10 ), chinese medicine,the temperature of the group and its(n = 10),western medicine group ,ramipril(n = 10). Model groups, traditional chinese medicine, and western medicine group continue to feed with high fat feed .Experiment end in drug intervention 4 weeks later.
Results:
1. Compared with the normal group, blood sugar, cardiac function, blood fat and NO content in serum of the rats in the model group are significantly raised, SOD content in serum is significantly lowered, there are statistical significances.
2. Compared with the model group, blood sugar, cardiac function, blood fat and NO content in serum of the rats in the administration groups are significantly lowered, and SOD content in serum is significantly raised.
3. There are no statistically significant difference in accordance with the comparison of blood sugar, cardiac function, blood fat and NO, SOD contents in serum of the rats among the administration groups.
Conclusion:
1. The experimental rat DCM (dilated cardiomyopathy) model, established based on the method for feeding high fat diet to rats (GK rats) with spontaneous non-obesity Type 2 diabetes mellitus, could aggravate their myocardial tissue injury, which is similar to the onset and development of human DCM.
2. The qi-supplementing and Yang-warming compound can suppress NO content in serum of the rats (GK rats) with spontaneous non-obesity Type 2 diabetes mellitus and lower the level of blood sugar and blood fat of the rats, and can also enhance the expression of SOD content in serum. Prompt: the qi-supplementing and Yang-warming compound can used for the prevention and treatment of DCM.
3. The DCM traditional Chinese medicine disease could cause blood vessel blockage and heart vessel blockage stasis after a long time because of long-term delay of thirst relieving, viscera dysfunction and yin and yang qi-blood injury, further turning into this disease. The key of its pathogenesis lies in deficiency of qi and yang, so the fundamental rule for the treatment of this disease shall fall upon qi supplementing and yang warming.
引文
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