脂多糖对人牙周膜干细胞一氧化氮表达的影响
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摘要
目的：
研究人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)在脂多糖(Lipopolysaccharides,LPS)刺激下产生一氧化氮(Nitric oxide,NO)的能力及一氧化氮合酶(Nitric Oxide Synthase,NOS),干扰素诱导蛋白10(IP-10)的表达情况,为探讨牙周膜干细胞在免疫调节中的作用机制提供前期研究基础。
方法：
收集临床上12-18岁因正畸拔除的健康无龋的前磨牙50例,刮取根中部的牙周膜,采用组织块直接贴壁培养法体外分离培养hPDLSCs,并进行牙周膜干细胞的鉴定。取对数生长期的4-8代细胞用于后续实验。实验分为对照组、LPS组(1ng/ml、10ng/ml、100ng/ml、1000ng/ml、10000ng/ml)及L-单甲基精氨酸(NG-Monomethyl-L-arginine,Monoacetate Salt, L-NMMA)组(10ng/ml LPS+100μmol/L L-NMMA)。细胞在37℃、5%CO2培养3h、6h、12h、24h后硝酸还原酶法检测细胞培养上清液中NO含量,ELISA法检测细胞培养上清液中IP-10的表达；免疫组化检测刺激前后牙周膜干细胞iNOS、nNOS、eNOS的表达,RT-PCR检测细胞中iNOS mRNA的表达。
结果：
1.组织块法培养人牙周膜细胞成功率高,表达人牙周膜干细胞标志STRO-1和CD146,具有骨向和脂肪向等多向分化的潜能,具备干细胞的特性。
2.不同浓度LPS处理hPDLSCs后,hPDLSCs产生的NO的浓度随时间增加而增加,在24h时达到高峰；hPDLSCs产生的NO的量随着LPS浓度升高而增加；加入L-NMMA可使NO产生浓度降低(P<0.05)。
3.免疫组化显示,不同浓度LPS处理hPDLSCs后,iNOS的中度表达,主要表达在hPDLSCs胞浆中,而eNOS、nNOS在未处理组和处理组均见中等程度表达,无显著差异。L-NMMA和LPS共处理组仍表达iNOS.
4. RT-PCR结果显示LPS刺激3h时iNOS mRNA增多。
5. ELISA显示,不同浓度LPS处理hPDLSCs后,上清液中IP-10的含量随时间增加而增加,在48h时达到高峰,随着LPS浓度的增加而降低。
结论：
1.LPS作用下,hPDLSCs产生NO的能力在一定时间内逐渐增强。
2. hPDLSCs产生NO主要是因为hPDLSCs中iNOS表达增多。
3. L-NMMA不抑制iNOS mRNA的表达,但可能抑制iNOS的活性。
4.LPS刺激hPDLSCs产生IP-10,含量随时间增加而增加。
Objective:
To investigate the immunological effects of human periodontal ligament stem cells(hPDLSCs) on NO expression in lipopolysaccharides(LPS)/and L-NMMA.
Methods:
Fifty health premolars from patients between 12 to 18 years old were extracted for orthodontic reasons, periodontal tissues were isolated and cultured in vitro to obtain hPDLSCs. Cells in 4th-8th passage cells were used for the following experiment. Several groups was devided as follow:control group with none LPS, LPS groups(lng/ml、10ng/ml、100ng/ml、1000ng/ml、10000ng/ml)and L-NMMA group(10ngLPS+100μmol/L L-NMMA).Cells were cultured at 37℃in 5% CO2 for 3hours、6 hours、12 hours、24 hours and 28 hours. The release of NO was measured by nitric acid deoxidize enzyme in the medium.Then the amounts of IP-10 in the supernatant were determined by an enzyme-linked immunosorbent assay (ELISA) respectively. The expression of iNOS, nNOSand eNOS was determined with immunohistochemistry. The mRNA of iNOS were analyzed by RT-PCR assay.
Results:
1. hPDLSCs were obtained in vitro successfully by tissue culture, hPDLSCs can espress STRO-1 and CD146. hPDLSCs had the potential of osteogenesis and adipogenic under some appropriate culture situation.
2. The release of NO increased along with the concentration of LPS. The release of NO increased with time, arrived the peak level at 24h, and then decreased at 28h.The release of NO in L-NMMA group decreased compared with the LPS group(10μg/ml) absolutely(P<0.05).
3. The expression of nNOSand eNOS was obvious and there is no significant difference before and after LPS stimulation. The expression of iNOS was observed with LPS stimulated. The L-NMMA group also express iNOS.
4. The mRNA of iNOS increased 3h after LPS stimulated, before the result of IHC.
5. By ELISA, The release of IP-10 increased with time, arrived the mountain level at 48h, but decreased along with the concentration of LPS.
Conclusions:
1. The ability of NO production in hPDLSCs increased with LPS stimulated in 24 hours.
2. The release of NO in hPDLSCs was due to the expression of inducible NOS with LPS stimulated.
3. L-NMMA could not inhibit it's mRNA expression, but might inhibited the activity of iNOS.
4. hPDLSCs stimulated by LPS could release IP-10, and IP-10 increased with time.
研究人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)在脂多糖(Lipopolysaccharides,LPS)刺激下产生一氧化氮(Nitric oxide,NO)的能力及一氧化氮合酶(Nitric Oxide Synthase,NOS),干扰素诱导蛋白10(IP-10)的表达情况,为探讨牙周膜干细胞在免疫调节中的作用机制提供前期研究基础。
方法：
收集临床上12-18岁因正畸拔除的健康无龋的前磨牙50例,刮取根中部的牙周膜,采用组织块直接贴壁培养法体外分离培养hPDLSCs,并进行牙周膜干细胞的鉴定。取对数生长期的4-8代细胞用于后续实验。实验分为对照组、LPS组(1ng/ml、10ng/ml、100ng/ml、1000ng/ml、10000ng/ml)及L-单甲基精氨酸(NG-Monomethyl-L-arginine,Monoacetate Salt, L-NMMA)组(10ng/ml LPS+100μmol/L L-NMMA)。细胞在37℃、5%CO2培养3h、6h、12h、24h后硝酸还原酶法检测细胞培养上清液中NO含量,ELISA法检测细胞培养上清液中IP-10的表达；免疫组化检测刺激前后牙周膜干细胞iNOS、nNOS、eNOS的表达,RT-PCR检测细胞中iNOS mRNA的表达。
结果：
1.组织块法培养人牙周膜细胞成功率高,表达人牙周膜干细胞标志STRO-1和CD146,具有骨向和脂肪向等多向分化的潜能,具备干细胞的特性。
2.不同浓度LPS处理hPDLSCs后,hPDLSCs产生的NO的浓度随时间增加而增加,在24h时达到高峰；hPDLSCs产生的NO的量随着LPS浓度升高而增加；加入L-NMMA可使NO产生浓度降低(P<0.05)。
3.免疫组化显示,不同浓度LPS处理hPDLSCs后,iNOS的中度表达,主要表达在hPDLSCs胞浆中,而eNOS、nNOS在未处理组和处理组均见中等程度表达,无显著差异。L-NMMA和LPS共处理组仍表达iNOS.
4. RT-PCR结果显示LPS刺激3h时iNOS mRNA增多。
5. ELISA显示,不同浓度LPS处理hPDLSCs后,上清液中IP-10的含量随时间增加而增加,在48h时达到高峰,随着LPS浓度的增加而降低。
结论：
1.LPS作用下,hPDLSCs产生NO的能力在一定时间内逐渐增强。
2. hPDLSCs产生NO主要是因为hPDLSCs中iNOS表达增多。
3. L-NMMA不抑制iNOS mRNA的表达,但可能抑制iNOS的活性。
4.LPS刺激hPDLSCs产生IP-10,含量随时间增加而增加。
Objective:
To investigate the immunological effects of human periodontal ligament stem cells(hPDLSCs) on NO expression in lipopolysaccharides(LPS)/and L-NMMA.
Methods:
Fifty health premolars from patients between 12 to 18 years old were extracted for orthodontic reasons, periodontal tissues were isolated and cultured in vitro to obtain hPDLSCs. Cells in 4th-8th passage cells were used for the following experiment. Several groups was devided as follow:control group with none LPS, LPS groups(lng/ml、10ng/ml、100ng/ml、1000ng/ml、10000ng/ml)and L-NMMA group(10ngLPS+100μmol/L L-NMMA).Cells were cultured at 37℃in 5% CO2 for 3hours、6 hours、12 hours、24 hours and 28 hours. The release of NO was measured by nitric acid deoxidize enzyme in the medium.Then the amounts of IP-10 in the supernatant were determined by an enzyme-linked immunosorbent assay (ELISA) respectively. The expression of iNOS, nNOSand eNOS was determined with immunohistochemistry. The mRNA of iNOS were analyzed by RT-PCR assay.
Results:
1. hPDLSCs were obtained in vitro successfully by tissue culture, hPDLSCs can espress STRO-1 and CD146. hPDLSCs had the potential of osteogenesis and adipogenic under some appropriate culture situation.
2. The release of NO increased along with the concentration of LPS. The release of NO increased with time, arrived the peak level at 24h, and then decreased at 28h.The release of NO in L-NMMA group decreased compared with the LPS group(10μg/ml) absolutely(P<0.05).
3. The expression of nNOSand eNOS was obvious and there is no significant difference before and after LPS stimulation. The expression of iNOS was observed with LPS stimulated. The L-NMMA group also express iNOS.
4. The mRNA of iNOS increased 3h after LPS stimulated, before the result of IHC.
5. By ELISA, The release of IP-10 increased with time, arrived the mountain level at 48h, but decreased along with the concentration of LPS.
Conclusions:
1. The ability of NO production in hPDLSCs increased with LPS stimulated in 24 hours.
2. The release of NO in hPDLSCs was due to the expression of inducible NOS with LPS stimulated.
3. L-NMMA could not inhibit it's mRNA expression, but might inhibited the activity of iNOS.
4. hPDLSCs stimulated by LPS could release IP-10, and IP-10 increased with time.
引文
[1]Dkuda E, Kaxot, Ishihara K.Involvement of periodontal pathic biofilm in vascular disease.Oral D.S,2004,10(1):5-12.
[2]Seo B M, Miura M, Gronthos S, et al.Investigation of multipotent postnatal stem cells from Human periodontal ligament.Lancet,2004,364(9429):149-155.
[3]Liu Yi, Zheng Ying, Ding Gang, et al.Periodontal Ligament Stem Cell-Mediated Treatment for Periodontitis in Miniature Swine.Stem Cells,2008,26(4):1065-1073.
[4]Anggard E. Nitric oxide:mediator, murderer and medicine. Lancet,1994,343 (8907):1199-1206.
[5]陈桂珍,孙伟莲.牙周炎患者唾液一氧化氮的含量及其与牙周病指标相关性的研究.华西口腔医学杂志,1999,17(2)：140-141.
[6]陈桂珍,孙伟莲.牙周病患者唾液中NO含量的研究.科技通报,1999,15(2)：149-151.
[7]Huang Shi-guang, Xie Min, Lv Fang-li.Change of nitric oxide concentration in ligature-induced periodontitis in rats中国病理生理杂志,2005,21(12)：2470-2473.
[8]林绿标,章翔,吴景文,等.小鼠神经干细胞中一氧化氮合成酶的表达.第四军医大学学报,2002,23(23)：2160-2163.
[9]Peunova N, Scheinker V, Cline H, et al.Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain.J Neurosci,2001,21(22):8809-8818.
[10]Mais A, Klein T, Ullrich V, et al.Prostanoid pattern and iNOS expression during chondrogenic differentiation of human mesenchymal stem cells.J Cell Biochem,2006,98(4):798-809.
[11]Wei Pan, Zhou-Sheng xiao, Quarles L D, et al.Genistein stimulates the osteoblastic differentiation via NO/cGMP in bone marrow culture.J Cell Biochem,2005,94(2):307-316.
[12]Takeichi O, Saito I, Okmnoto Y, et al. Cytokine regulation on the synthesis of nitric oxide in vivo by chronically infected human polymorphonuclear leucocytes. Immunology,1998,93(2):275-280.
[13]Ren G, Zhang L, Zhao X, et al.Mesenchymal Stem Cell-Mediated Immunos- uppression Occurs via Concerted Action of Chemokines and Nitric Oxide.Cell Stem Cell,2008,2(2):141-150.
[14]Zhang Qunzhou, Shi Shihong, Liu Yi, et al.Mesenchymal Stem Cells Derived from Human Gingiva Are Capable of Immunomodulatory Functions and Ameliorate Inflammation-Related Tissue Destruction in Experimental Colitis. J. Immunol,2009,183(12):7787-7798.
[15]陈希哲,林云锋,乔鞠,等.人体脂肪基质细胞分离培养及其成骨潜能.实用口腔医学杂志,2004,20(1)：12-15.
[16]Meirelles LS, Nardi NB.Murine marrowderived mesenchymal stem cell:isolati-on,in vitro expansion,and characterization.Br J Haematol,2003,123(4):702-711.
[17]Kawada H, Fujita J, Kinjo K, et al.Nonhematopoietic mesenchymal stem cells can be mobilized and differentiate into cardiomyocytes after myocardial infarction. Blood,2004,104(12):3581-3587.
[18]Gronthos S., Mankani M., Brahim J., et al.Postnatal human dental pulp stem cells (DPSCs) in vitro and invivo.Proc Natl Acad Sci USA,2000,97(25):13625-13630.
[19]Sun Lingyun, Kentaro A, Zhang Huayong, et al. Mesenchymal Stem Cell Transplantation Reverses Multiorgan Dysfunction in Systemic Lupus Erythemato-sus Mice and Humans.Stem Cells,2009,27(6):1421-1432.
[20]章翔,林绿标,姬西团,等.外源性一氧化氮对小鼠神经干细胞增殖的抑制作用.第四军医大学学报,2004,25(4)：297-300.
[21]Sato K, K Ozaki, I Oh, et al.Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells.Blood,2007,109(1):228-234.
[22]Orciania M, Trubianib O, Vigninic A, et al.Nitric oxide production during the osteogenic differentiation of human periodontal ligament mesenchymal stem cells.Acta histochemica,2009,111(1):15-24.
[23]Takeichi O, Saito I, Okmnoto Y, et al. Cytokine regulation on the synthesis of nitric oxide in vivo by chronically infected human polymorphonuclear leucocytes. Immunology,1998,93(2):275-280.
[24]Gabor G, Allon N.Spectrofluorometric Method For NO Determination. Anal Bioc-hem,1994,220(1):16-19.
[25]Stone JR, Marletta MA.Spectral and kinetic studies on the activation of soluble guanylate cyclase by nitric oxide.Biochemistry,1996 Jan 30,35(4):1093-1099.
[26]陈桂珍,孙伟莲.牙周炎患者唾液一氧化氮的含量及其与牙周病指标相关性的研究.华西口腔医学杂志,1999,17(2)：140-141.
[27]刘建国,王宪,陈明哲.一氧化氮和细胞因子之间的相互调节作用.生理科学进展,2000,31(1)：61-64.
[28]邱洪晟,刘漪,,伏建峰,等.127名汉族青年口腔唾液一氧化氮含量检测.牙体牙髓牙周病学杂志,2000,10(2)：76-77.
[29]段慧琴,乔健,张卫东,等.LPS对大鼠肠黏膜微血管内皮细胞分泌NO的影响.畜牧兽医学报,2005,36(9)：974-976.
[30]王海波,秦永伟,程纯,等.LPS对大鼠雪旺氏细胞iNOS表达的影响.中国免疫学杂志,2007,23,(9)：815-818.
[31]Kerezoudis NP, Olgart L, Fried K.Localization of NADPH-diaphorase activity in the dental pulp, periodontium and alveolar bone of the rat.Histochem J,1993,100(4):319-322.
[32]佟丹,陈世璋.健康人牙龈组织中一氧化氮的分布.北京口腔医学,1997,5(3)：130-130.
[33]尚政军,李金荣,李祖兵,等.口腔鳞状细胞癌中内皮型一氧化氮合酶基因表达的体外研究.中国口腔颌面外科杂志,2003,1(3)：175-178.
[34]杨萍,高文信,魏秀峰,等.口腔癌前病变、口腔鳞癌中诱导型一氧化氮合酶与p53蛋白表达及细胞增殖相互关系的研究.临床口腔医学杂志,2004,20(3)：143-145.
[35]曾丽苹,韩梅,温进坤.脂多糖对血管平滑肌细胞一氧化氮合酶基因表达的影响.河北医科大学学报,1999,3(20)：137-139.
[36]闻平,戴庚孙.细菌脂多糖对成纤维细胞iNOS活性及细胞增殖的影响.中国人兽共患病杂志,1998,14(6)：19-22.
[37]Jonsson D, Nebel D, Bratthall G, et al.LPS-induced MCP-1 and IL-6 production is not reversed by oestrogen in human periodontal ligament cells.Archives of Oral Biology,2008,9(53):896-902.
[38]王海波,秦永伟,程纯,等.LPS对大鼠雪旺氏细胞iNOS表达的影响.中国免疫学杂志,2007,23(9)：815-818.
[39]邹淑娟,胡静,高占巍,等.IL-1及L-NMMA对兔裸状突软骨细胞NO生成和 iNOS表达的影响.临床口腔医学杂志,2002,18(5)：345-346
[40]Bodis S., Haregewoin A.Evidence for the Release and Possible Neural Regulation of Nitric Oxide in Human Saliva.Biochem Biophys Res Commun,1993,194(1): 347-350.
[41]Miyamaru M, Kato C, Saito K, et al.Nitric oxide production of normal and immune mice macrophages against periodontopathic bacteria, and bactericide effect of SNAP generated nitric oxide.Chemical Abstracts,1999,131(3):458-460.
[42]Komatsu T, Srivastava N, Revzin M, et al.Mechanisms of Cytokine-Mediated Inhibition of Viral Replication. Virology,1999,259 (2):334-341.
[43]Moncada S, Palmer RM, Higgs EA.Nitric oxideL:physiology, pathophysiology, and pharmacol-ogy. Pharmacol Rev,1991,43(2):109-142.
[44]陶人川.一氧化氮在口腔疾病中的研究现状与展望.广东牙病防治,1999,7(3)：236-237.
[45]Takeichi O, Saito I, Okmnoto Y, et al. Cytokine regulation on the synthesis of nitric oxide in vivo by chronically infected human polymorphonuclear leucocytes. Immunology,1998,93(2):275-280.
[46]毛钊,杨俭,曹永安,等.老年牙周炎患者牙龈组织NO水平研究.实用口腔医学杂志,2001,17(5)：397-398.
[47]毛钊,杨俭,吴织芬,等.老年牙周炎牙龈组织诱导型一氧化氮合酶分布研究.实用口腔医学杂志,2003,19(4)：329-332.
[48]毛钊,杨俭,赖仁胜.老年人牙周炎龈组织内T和B淋巴细胞免疫组织化学研究.牙体牙髓牙周病学杂志,1998,8(4)：240-240.
[49]Demetris AJ, Murase N, Lee RG, et al.Chronic rejection.A general overview of histopathology and pathophysiology with emphasis on liver, heart and intestinal allografts.Ann Transplant,1997,2(2):27-44.
[50]Luster A D.Gamma-interferon transcriptionally regulates an early-response gene containing homology toplateletproteins[J].Nature,1985,315(6021):672-676.
[51]喻三红,熊思东.干扰素诱导蛋白-10介导的Thl型炎症反应.生命的化学,2001,22(5)：432-435.
[52]Knechtle SJ.Rejection of the liver trnasplant.Semin Gastorintest Dis,1998,9(3): 126-135.
[53]Aggarwal S, M. F. Pittenger. Human mesenchymal stem cells modulate allogeneic immune cell responses.Blood,2005,105(4):1815-1822.
[54]Ryan, J M, F Barry, J M Murphy, et al. Interferon-y does not break, but promotes the immunosuppressive capacity of adult human mesenchymal stem cells. Clin Exp Immunol,2007,149(2):353-363.
[55]Krampera M, L Cosmi, R Angeli, et al.Role for interferon-y in the immunomodulatory activity of human bone marrow mesenchymal stem cells. Stem Cells,2006,24(2):386-398.
[56]Delarosa O, E Lombardo, A Beraza, et al.Requirement of IFN-y-mediated indoleamine 2,3-dioxygenase expression in the modulation of lymphocyte proliferation by human adiposederived stem cells.Tissue Eng Part A,October 2009,15(10):2795-2806.
[57]Spaggiari G. M, A Capobianco, H Abdelrazik, et al.Mesenchymal stem cells inhibit natural killer-cell proliferation, cytotoxicity, and cytokine production:role of indoleamine 2,3-dioxygenase and prostaglandin E2.Blood,2008 Febl,111(3): 1327-1333.
[58]Sato K, K Ozaki, I Oh, et al.Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells.Blood,2007,109(1):228-234.
[59]E Gemmell, C L Carter, Seymour GJ.Chemokines in human periodontal disease tissues.Clin Exp Immunol,2001,125(1):134-141.
[60]Simpson JE, Newcombe J, Cuzner ML, et al.Expression of the interferon-gamma-inducible chemokines IP-10 and Mig and their receptor, CXCR3, in multiple sclerosis lesions. Neuropatholo Appl Neurobiol,2000,26(2):133-142.
[61]Hosokawa Y, Hosokawa I, Ozaki K, et al.Cytokines differentially regulate CXC L 10 production by interferon-y-stimulated or tumor necrosis factor-a-stimulated human gingival fibroblasts.J Periodont Res,2009,44(2):225-231.
[62]Ovstebo R, Olstad OK, Brusletto B, et al.Identification of Genes Particularly Sensitive to Lipopolysaccharide (LPS) in Human Monocytes Induced by Wild-Type versus LPS-Deficient Neisseria meningitidis Strains.INFECTION AND IMMUNITY,June 2008,76(6):2685-2695.
[1]Snysder SH, Bredt DS.Sci Am,1992;may:68-77.
[2]朱涛.一氧化氮——新的细胞信使.国外医学生理、病理科学和临床分册,1994,14(4)：212-214.
[3]Palmer RM, Ferrige AG, Moncada.Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor.Nature,1987,327(6122):524-526.
[4]Anggard E. Nitric oxide:mediator, murderer and medicine.Lancet,1994,343 (8907):1199-1206.
[5]Palmer RMJ, et al.Nature,1988,333:664-666.
[6]Nicotera P, Brune, Bagetta GNitric oxide:inducer or suppressor of apoptosis? Trends Pharmacol Sci,1997,18(6):189-190.
[7]Bates JN, Baker MT, Guerra R Jr, et al.Nitric oxide generation from nitr-oprusside by vascular tissue:Evidence that reduction of the nitropruss-ide anion and cyanide loss are required.Biochem Pharmacol,1991,42(suppl):s157-s165.
[8]Nathan c, Xie QW.Nitric oxide synthase:roles, tolls, and controls.Cell,1994,78(6): 915-918.
[9]刘建国,王宪,陈明哲.一氧化氮和细胞因子之间的相互调节作用.生理科学进展,2000,31(1)：61-64.
[10]Stone JR, marletta MA. Spectral and kinetil studies on the activation of solube guanglate cyelase by nitrix oxide.Biochemistry,1996,35:1093-1099.
[11]杜佳梅,陶智潞,张松林.组织细胞中一氧化氮含量测定的现况.中国煤炭工业医学杂志,1998,1(6)：484-485.
[12]Gabor G., Allon N.Spectrofluorometric Method for NO Determination.Anal Biochem,1994,220(1):16-19.
[13]Carberg M. Assay of neuronal nitric oxide synthase by HPLC determination of citrulline.J Neurosci methods,1994,52(2):165-167.
[14]Kaks S, Tanaka M, Muramatsu M, et al.Determination of nitrite by high-performance liquid chromatography system with electrochemical detector: Measurement of nitric oxide synthase activity in rat cerebellum cytosol.Biomed Chromatogr,1994,8(1):14-18.
[15]严焱,刘正.大鼠实验性牙髓炎组织中诱导型一氧化氮合成酶的mRNA表达.牙体牙髓牙周病杂志,1998,8(4)：234-236.
[16]包柳郁.诱导型一氧化氮合成酶与尖周病变.国外医学口腔医学分册,2000,27(5)：275-278.
[17]Kerezoudis NP, Olgart L, Fried K.Localization of NADPH-diaphorase activity in the dental pulp, periodontium and alveolar bone of the rat.Histochem J,1993, 100(4):319-322.
[18]佟丹,陈世璋.健康人牙龈组织中一氧化氮的分布.北京口腔医学,1997,5(3)：130-130.
[19]陈世障,吴敬,王石麟,等.正常人与慢性牙周炎患者牙龈组织中免疫活性物质的观察,Chin J Pathol,1996,25(6)：340-342.
[20]Green SJ.Nitric oxide in mucosal immunity.Nat Med,1995,1(6):515-517.
[21]Bodis S, Haregewoin A.Evidence for the Release and Possible Neural Regulation of Nitric Oxide in Human Saliva.Biochem Biophys Res Commun,1993,194(1): 347-350.
[22]Miyamaru M, Kato M, Saito K, et al.Nitric oxide production of normal and immune mice macrophages against periodontopathic bacteria, and bactericide effect of SNAP generated nitric oxide.Chemical Abstracts.1999,131(3):458-460.
[23]Komatsu T, Srivastava N, Revzin M, et al.Mechanisms of Cytokine-Mediated Inhibition of Viral Replication.Virology,1999,259 (2):334-341.
[24]Moncada S, Palmer RM, Higgs EA.Nitric oxideL:physiology, pathophysiology, and pharmacol-ogy. Pharmacol Rev,1991,43(2):109-142.
[25]陶人川.一氧化氮在口腔疾病中的研究现状与展望.广东牙病防治,1999,7(3)：236-237.
[26]毛钊,杨俭,曹永安,等.老年牙周炎患者牙龈组织NO水平研究.实用口腔医学杂志,2001,17(5)：397-398.
[27]毛钊,杨俭,吴织芬,等.老年牙周炎牙龈组织诱导型一氧化氮合酶分布研究.实用口腔医学杂志,2003,19(4)：329-332.
[28]毛钊,杨俭,赖仁胜.老年人牙周炎龈组织内T和B淋巴细胞免疫组织化学研究.牙体牙髓牙周病学杂志,1998,8(4)：240-240.
[29]张听,陈世璋.人牙龈组织中内皮素及一氧化氮合成酶的异常与牙周病相关 性的研究.北京口腔医学,1999,7(2)：63-65.
[30]徐立平,任萍,张丽琨,等.硝酸钾在口腔微环境中的降解.第三届国际天然药与微生态学术研讨会论文集,2000,7：254-255.
[31]陈桂珍,孙伟莲.牙周炎患者唾液一氧化氮的含量及其与牙周病指标相关性的研究.华西口腔医学杂志,1999,17(2)：140-141.
[32]陈桂珍,孙伟莲.牙周病患者唾液中NO含量的研究.科技通报,1999,15(2)：149-151.
[33]Huang Shi-guang, Xie Min, Lv FangG-li.Change of nitric oxide concentration in ligature-induced periodontitis in rats中国病理生理杂志,2005,21(12)：2470-2473.
[34]邱洪晟,刘漪,伏建峰,等.127名汉族青年口腔唾液一氧化氮含量检测.牙体牙髓牙周病学杂志,2000,10(2)：76-77.
[35]Kankanian AL, Akopov SE.The stimulation of nitric oxide synthesis as a possible protective function of the saliva and its disorder in periodontal diseases.Stomat-ologiia,1996,75(3):19-21.
[36]Takeichi O, Saito I, Okmnoto Y, et al.Lear leukocytes as a mechamism for the development of periodontal lesions.Stomatologiia,1996,75(2):12-14.
[37]Takeichi O, Saito I, Okmnoto Y, et al.Cytokine regulation on the synthesis of nitric oxide in vivo by chronically infected human polymorphonuclear leucocytes. Immunology,1998,93(2):275-280.
[38]许强.一氧化氮及其合成酶在颞下颌关节紊乱病中的作用.国外医学口腔医学分册,2001,28(2)：90-93.
[39]徐芸主译.口腔正畸学—现代原理与技术[M].天津：天津科技翻译出版公司,1997,365-374.
[40]裴秀洁.一氧化氮在口腔正畸学中的研究进展.口腔医学研究,2004,5：558-559.
[41]Chae HJ, Park PK, Chung HT, et al. Nitric oxide is a regulator of bone remodelling.J Pharm Pharmacol,1997,49(5):897-902.
[42]傅民魁.口腔正畸学[M].第4版.北京：人民卫生出版社,2003,165-172.
[43]Shirazi M, Nilforoushan D, Alghasi H, et al.The role of nitric oxide in orthodontic tooth movement in rats. Angle Orthod,2002,72(3):211-215.
[44]裴秀洁,林桂书.大鼠正畸过程牙周组织中NOS三种同形异构体的表达及意义.河北医科大学学报,2005,26(6)：436-439.
[45]尚政军,李金荣,李祖兵,等.口腔鳞状细胞癌中内皮型一氧化氮合酶基因表达的体外研究.中国口腔颌面外科杂志,2003,1(3)：175-178.
[46]杨萍,高文信,魏秀峰,等.口腔癌前病变、口腔鳞癌中诱导型一氧化氮合酶与p53蛋白表达及细胞增殖相互关系的研究.临床口腔医学杂志,2004,20(3)：143-145.
[47]Hilbig H, Franke H, Bidmon H, et al.Nitric oxide synthase isoenzymes during in vitro development of rat neuronal and human non-neuronal cells.Neurosci Lett, 2001,297(1):9-12.
[48]Peunova N, Enikolopov GNitric oxide triggers a switch to growth arrest during differentiation of neuronal cells. Nature,1995,375(6526):68-73.
[49]林绿标,章翔,吴景文,等.小鼠神经干细胞中一氧化氮合成酶的表达.第四军医大学学报,2002,23(23)：2160-2163.
[50]Peunova N, Scheinker V, Cline H, et al.Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain.J Neurosci,2001,21 (22):8809-8818.
[51]Mais A, Klein T, Ullrich V, et al.Prostanoid pattern and iNOS expression during chondrogenic differentiation of human mesenchymal stem cells.J Cell Biochem, 2006,98(4):798-809.
[52]章翔,林绿标,姬西团,等.外源性一氧化氮对小鼠神经干细胞增殖的抑制作用,第四军医大学学报,2004,25(4)：297-300.
[53]林绿标,章翔,姬西团,等.外源性一氧化氮对神经干细胞分化的促进作用.第四军医大学学报,2004,25(4)：301-303.
[54]Wei Pan, Quarles L D, Song LH, et al.Genistein stimulates the osteoblastic differentiation via NO/cGMP in bone marrow culture. J Cell Biochem,2005,94(2): 307-316.
[55]Evans DM, Ralston SH.Nitric oxide and bone.J Bone Miner Res,1996,11(3): 300-305.
[56]金晓明,李雅馨,李金荣,等.外源性转化生长因子β1对口腔鳞状细胞癌细胞系一氧化氮含量的影响.现代口腔医学杂志,2003,17(3)：206-208.
[2]Seo B M, Miura M, Gronthos S, et al.Investigation of multipotent postnatal stem cells from Human periodontal ligament.Lancet,2004,364(9429):149-155.
[3]Liu Yi, Zheng Ying, Ding Gang, et al.Periodontal Ligament Stem Cell-Mediated Treatment for Periodontitis in Miniature Swine.Stem Cells,2008,26(4):1065-1073.
[4]Anggard E. Nitric oxide:mediator, murderer and medicine. Lancet,1994,343 (8907):1199-1206.
[5]陈桂珍,孙伟莲.牙周炎患者唾液一氧化氮的含量及其与牙周病指标相关性的研究.华西口腔医学杂志,1999,17(2)：140-141.
[6]陈桂珍,孙伟莲.牙周病患者唾液中NO含量的研究.科技通报,1999,15(2)：149-151.
[7]Huang Shi-guang, Xie Min, Lv Fang-li.Change of nitric oxide concentration in ligature-induced periodontitis in rats中国病理生理杂志,2005,21(12)：2470-2473.
[8]林绿标,章翔,吴景文,等.小鼠神经干细胞中一氧化氮合成酶的表达.第四军医大学学报,2002,23(23)：2160-2163.
[9]Peunova N, Scheinker V, Cline H, et al.Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain.J Neurosci,2001,21(22):8809-8818.
[10]Mais A, Klein T, Ullrich V, et al.Prostanoid pattern and iNOS expression during chondrogenic differentiation of human mesenchymal stem cells.J Cell Biochem,2006,98(4):798-809.
[11]Wei Pan, Zhou-Sheng xiao, Quarles L D, et al.Genistein stimulates the osteoblastic differentiation via NO/cGMP in bone marrow culture.J Cell Biochem,2005,94(2):307-316.
[12]Takeichi O, Saito I, Okmnoto Y, et al. Cytokine regulation on the synthesis of nitric oxide in vivo by chronically infected human polymorphonuclear leucocytes. Immunology,1998,93(2):275-280.
[13]Ren G, Zhang L, Zhao X, et al.Mesenchymal Stem Cell-Mediated Immunos- uppression Occurs via Concerted Action of Chemokines and Nitric Oxide.Cell Stem Cell,2008,2(2):141-150.
[14]Zhang Qunzhou, Shi Shihong, Liu Yi, et al.Mesenchymal Stem Cells Derived from Human Gingiva Are Capable of Immunomodulatory Functions and Ameliorate Inflammation-Related Tissue Destruction in Experimental Colitis. J. Immunol,2009,183(12):7787-7798.
[15]陈希哲,林云锋,乔鞠,等.人体脂肪基质细胞分离培养及其成骨潜能.实用口腔医学杂志,2004,20(1)：12-15.
[16]Meirelles LS, Nardi NB.Murine marrowderived mesenchymal stem cell:isolati-on,in vitro expansion,and characterization.Br J Haematol,2003,123(4):702-711.
[17]Kawada H, Fujita J, Kinjo K, et al.Nonhematopoietic mesenchymal stem cells can be mobilized and differentiate into cardiomyocytes after myocardial infarction. Blood,2004,104(12):3581-3587.
[18]Gronthos S., Mankani M., Brahim J., et al.Postnatal human dental pulp stem cells (DPSCs) in vitro and invivo.Proc Natl Acad Sci USA,2000,97(25):13625-13630.
[19]Sun Lingyun, Kentaro A, Zhang Huayong, et al. Mesenchymal Stem Cell Transplantation Reverses Multiorgan Dysfunction in Systemic Lupus Erythemato-sus Mice and Humans.Stem Cells,2009,27(6):1421-1432.
[20]章翔,林绿标,姬西团,等.外源性一氧化氮对小鼠神经干细胞增殖的抑制作用.第四军医大学学报,2004,25(4)：297-300.
[21]Sato K, K Ozaki, I Oh, et al.Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells.Blood,2007,109(1):228-234.
[22]Orciania M, Trubianib O, Vigninic A, et al.Nitric oxide production during the osteogenic differentiation of human periodontal ligament mesenchymal stem cells.Acta histochemica,2009,111(1):15-24.
[23]Takeichi O, Saito I, Okmnoto Y, et al. Cytokine regulation on the synthesis of nitric oxide in vivo by chronically infected human polymorphonuclear leucocytes. Immunology,1998,93(2):275-280.
[24]Gabor G, Allon N.Spectrofluorometric Method For NO Determination. Anal Bioc-hem,1994,220(1):16-19.
[25]Stone JR, Marletta MA.Spectral and kinetic studies on the activation of soluble guanylate cyclase by nitric oxide.Biochemistry,1996 Jan 30,35(4):1093-1099.
[26]陈桂珍,孙伟莲.牙周炎患者唾液一氧化氮的含量及其与牙周病指标相关性的研究.华西口腔医学杂志,1999,17(2)：140-141.
[27]刘建国,王宪,陈明哲.一氧化氮和细胞因子之间的相互调节作用.生理科学进展,2000,31(1)：61-64.
[28]邱洪晟,刘漪,,伏建峰,等.127名汉族青年口腔唾液一氧化氮含量检测.牙体牙髓牙周病学杂志,2000,10(2)：76-77.
[29]段慧琴,乔健,张卫东,等.LPS对大鼠肠黏膜微血管内皮细胞分泌NO的影响.畜牧兽医学报,2005,36(9)：974-976.
[30]王海波,秦永伟,程纯,等.LPS对大鼠雪旺氏细胞iNOS表达的影响.中国免疫学杂志,2007,23,(9)：815-818.
[31]Kerezoudis NP, Olgart L, Fried K.Localization of NADPH-diaphorase activity in the dental pulp, periodontium and alveolar bone of the rat.Histochem J,1993,100(4):319-322.
[32]佟丹,陈世璋.健康人牙龈组织中一氧化氮的分布.北京口腔医学,1997,5(3)：130-130.
[33]尚政军,李金荣,李祖兵,等.口腔鳞状细胞癌中内皮型一氧化氮合酶基因表达的体外研究.中国口腔颌面外科杂志,2003,1(3)：175-178.
[34]杨萍,高文信,魏秀峰,等.口腔癌前病变、口腔鳞癌中诱导型一氧化氮合酶与p53蛋白表达及细胞增殖相互关系的研究.临床口腔医学杂志,2004,20(3)：143-145.
[35]曾丽苹,韩梅,温进坤.脂多糖对血管平滑肌细胞一氧化氮合酶基因表达的影响.河北医科大学学报,1999,3(20)：137-139.
[36]闻平,戴庚孙.细菌脂多糖对成纤维细胞iNOS活性及细胞增殖的影响.中国人兽共患病杂志,1998,14(6)：19-22.
[37]Jonsson D, Nebel D, Bratthall G, et al.LPS-induced MCP-1 and IL-6 production is not reversed by oestrogen in human periodontal ligament cells.Archives of Oral Biology,2008,9(53):896-902.
[38]王海波,秦永伟,程纯,等.LPS对大鼠雪旺氏细胞iNOS表达的影响.中国免疫学杂志,2007,23(9)：815-818.
[39]邹淑娟,胡静,高占巍,等.IL-1及L-NMMA对兔裸状突软骨细胞NO生成和 iNOS表达的影响.临床口腔医学杂志,2002,18(5)：345-346
[40]Bodis S., Haregewoin A.Evidence for the Release and Possible Neural Regulation of Nitric Oxide in Human Saliva.Biochem Biophys Res Commun,1993,194(1): 347-350.
[41]Miyamaru M, Kato C, Saito K, et al.Nitric oxide production of normal and immune mice macrophages against periodontopathic bacteria, and bactericide effect of SNAP generated nitric oxide.Chemical Abstracts,1999,131(3):458-460.
[42]Komatsu T, Srivastava N, Revzin M, et al.Mechanisms of Cytokine-Mediated Inhibition of Viral Replication. Virology,1999,259 (2):334-341.
[43]Moncada S, Palmer RM, Higgs EA.Nitric oxideL:physiology, pathophysiology, and pharmacol-ogy. Pharmacol Rev,1991,43(2):109-142.
[44]陶人川.一氧化氮在口腔疾病中的研究现状与展望.广东牙病防治,1999,7(3)：236-237.
[45]Takeichi O, Saito I, Okmnoto Y, et al. Cytokine regulation on the synthesis of nitric oxide in vivo by chronically infected human polymorphonuclear leucocytes. Immunology,1998,93(2):275-280.
[46]毛钊,杨俭,曹永安,等.老年牙周炎患者牙龈组织NO水平研究.实用口腔医学杂志,2001,17(5)：397-398.
[47]毛钊,杨俭,吴织芬,等.老年牙周炎牙龈组织诱导型一氧化氮合酶分布研究.实用口腔医学杂志,2003,19(4)：329-332.
[48]毛钊,杨俭,赖仁胜.老年人牙周炎龈组织内T和B淋巴细胞免疫组织化学研究.牙体牙髓牙周病学杂志,1998,8(4)：240-240.
[49]Demetris AJ, Murase N, Lee RG, et al.Chronic rejection.A general overview of histopathology and pathophysiology with emphasis on liver, heart and intestinal allografts.Ann Transplant,1997,2(2):27-44.
[50]Luster A D.Gamma-interferon transcriptionally regulates an early-response gene containing homology toplateletproteins[J].Nature,1985,315(6021):672-676.
[51]喻三红,熊思东.干扰素诱导蛋白-10介导的Thl型炎症反应.生命的化学,2001,22(5)：432-435.
[52]Knechtle SJ.Rejection of the liver trnasplant.Semin Gastorintest Dis,1998,9(3): 126-135.
[53]Aggarwal S, M. F. Pittenger. Human mesenchymal stem cells modulate allogeneic immune cell responses.Blood,2005,105(4):1815-1822.
[54]Ryan, J M, F Barry, J M Murphy, et al. Interferon-y does not break, but promotes the immunosuppressive capacity of adult human mesenchymal stem cells. Clin Exp Immunol,2007,149(2):353-363.
[55]Krampera M, L Cosmi, R Angeli, et al.Role for interferon-y in the immunomodulatory activity of human bone marrow mesenchymal stem cells. Stem Cells,2006,24(2):386-398.
[56]Delarosa O, E Lombardo, A Beraza, et al.Requirement of IFN-y-mediated indoleamine 2,3-dioxygenase expression in the modulation of lymphocyte proliferation by human adiposederived stem cells.Tissue Eng Part A,October 2009,15(10):2795-2806.
[57]Spaggiari G. M, A Capobianco, H Abdelrazik, et al.Mesenchymal stem cells inhibit natural killer-cell proliferation, cytotoxicity, and cytokine production:role of indoleamine 2,3-dioxygenase and prostaglandin E2.Blood,2008 Febl,111(3): 1327-1333.
[58]Sato K, K Ozaki, I Oh, et al.Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells.Blood,2007,109(1):228-234.
[59]E Gemmell, C L Carter, Seymour GJ.Chemokines in human periodontal disease tissues.Clin Exp Immunol,2001,125(1):134-141.
[60]Simpson JE, Newcombe J, Cuzner ML, et al.Expression of the interferon-gamma-inducible chemokines IP-10 and Mig and their receptor, CXCR3, in multiple sclerosis lesions. Neuropatholo Appl Neurobiol,2000,26(2):133-142.
[61]Hosokawa Y, Hosokawa I, Ozaki K, et al.Cytokines differentially regulate CXC L 10 production by interferon-y-stimulated or tumor necrosis factor-a-stimulated human gingival fibroblasts.J Periodont Res,2009,44(2):225-231.
[62]Ovstebo R, Olstad OK, Brusletto B, et al.Identification of Genes Particularly Sensitive to Lipopolysaccharide (LPS) in Human Monocytes Induced by Wild-Type versus LPS-Deficient Neisseria meningitidis Strains.INFECTION AND IMMUNITY,June 2008,76(6):2685-2695.
[1]Snysder SH, Bredt DS.Sci Am,1992;may:68-77.
[2]朱涛.一氧化氮——新的细胞信使.国外医学生理、病理科学和临床分册,1994,14(4)：212-214.
[3]Palmer RM, Ferrige AG, Moncada.Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor.Nature,1987,327(6122):524-526.
[4]Anggard E. Nitric oxide:mediator, murderer and medicine.Lancet,1994,343 (8907):1199-1206.
[5]Palmer RMJ, et al.Nature,1988,333:664-666.
[6]Nicotera P, Brune, Bagetta GNitric oxide:inducer or suppressor of apoptosis? Trends Pharmacol Sci,1997,18(6):189-190.
[7]Bates JN, Baker MT, Guerra R Jr, et al.Nitric oxide generation from nitr-oprusside by vascular tissue:Evidence that reduction of the nitropruss-ide anion and cyanide loss are required.Biochem Pharmacol,1991,42(suppl):s157-s165.
[8]Nathan c, Xie QW.Nitric oxide synthase:roles, tolls, and controls.Cell,1994,78(6): 915-918.
[9]刘建国,王宪,陈明哲.一氧化氮和细胞因子之间的相互调节作用.生理科学进展,2000,31(1)：61-64.
[10]Stone JR, marletta MA. Spectral and kinetil studies on the activation of solube guanglate cyelase by nitrix oxide.Biochemistry,1996,35:1093-1099.
[11]杜佳梅,陶智潞,张松林.组织细胞中一氧化氮含量测定的现况.中国煤炭工业医学杂志,1998,1(6)：484-485.
[12]Gabor G., Allon N.Spectrofluorometric Method for NO Determination.Anal Biochem,1994,220(1):16-19.
[13]Carberg M. Assay of neuronal nitric oxide synthase by HPLC determination of citrulline.J Neurosci methods,1994,52(2):165-167.
[14]Kaks S, Tanaka M, Muramatsu M, et al.Determination of nitrite by high-performance liquid chromatography system with electrochemical detector: Measurement of nitric oxide synthase activity in rat cerebellum cytosol.Biomed Chromatogr,1994,8(1):14-18.
[15]严焱,刘正.大鼠实验性牙髓炎组织中诱导型一氧化氮合成酶的mRNA表达.牙体牙髓牙周病杂志,1998,8(4)：234-236.
[16]包柳郁.诱导型一氧化氮合成酶与尖周病变.国外医学口腔医学分册,2000,27(5)：275-278.
[17]Kerezoudis NP, Olgart L, Fried K.Localization of NADPH-diaphorase activity in the dental pulp, periodontium and alveolar bone of the rat.Histochem J,1993, 100(4):319-322.
[18]佟丹,陈世璋.健康人牙龈组织中一氧化氮的分布.北京口腔医学,1997,5(3)：130-130.
[19]陈世障,吴敬,王石麟,等.正常人与慢性牙周炎患者牙龈组织中免疫活性物质的观察,Chin J Pathol,1996,25(6)：340-342.
[20]Green SJ.Nitric oxide in mucosal immunity.Nat Med,1995,1(6):515-517.
[21]Bodis S, Haregewoin A.Evidence for the Release and Possible Neural Regulation of Nitric Oxide in Human Saliva.Biochem Biophys Res Commun,1993,194(1): 347-350.
[22]Miyamaru M, Kato M, Saito K, et al.Nitric oxide production of normal and immune mice macrophages against periodontopathic bacteria, and bactericide effect of SNAP generated nitric oxide.Chemical Abstracts.1999,131(3):458-460.
[23]Komatsu T, Srivastava N, Revzin M, et al.Mechanisms of Cytokine-Mediated Inhibition of Viral Replication.Virology,1999,259 (2):334-341.
[24]Moncada S, Palmer RM, Higgs EA.Nitric oxideL:physiology, pathophysiology, and pharmacol-ogy. Pharmacol Rev,1991,43(2):109-142.
[25]陶人川.一氧化氮在口腔疾病中的研究现状与展望.广东牙病防治,1999,7(3)：236-237.
[26]毛钊,杨俭,曹永安,等.老年牙周炎患者牙龈组织NO水平研究.实用口腔医学杂志,2001,17(5)：397-398.
[27]毛钊,杨俭,吴织芬,等.老年牙周炎牙龈组织诱导型一氧化氮合酶分布研究.实用口腔医学杂志,2003,19(4)：329-332.
[28]毛钊,杨俭,赖仁胜.老年人牙周炎龈组织内T和B淋巴细胞免疫组织化学研究.牙体牙髓牙周病学杂志,1998,8(4)：240-240.
[29]张听,陈世璋.人牙龈组织中内皮素及一氧化氮合成酶的异常与牙周病相关 性的研究.北京口腔医学,1999,7(2)：63-65.
[30]徐立平,任萍,张丽琨,等.硝酸钾在口腔微环境中的降解.第三届国际天然药与微生态学术研讨会论文集,2000,7：254-255.
[31]陈桂珍,孙伟莲.牙周炎患者唾液一氧化氮的含量及其与牙周病指标相关性的研究.华西口腔医学杂志,1999,17(2)：140-141.
[32]陈桂珍,孙伟莲.牙周病患者唾液中NO含量的研究.科技通报,1999,15(2)：149-151.
[33]Huang Shi-guang, Xie Min, Lv FangG-li.Change of nitric oxide concentration in ligature-induced periodontitis in rats中国病理生理杂志,2005,21(12)：2470-2473.
[34]邱洪晟,刘漪,伏建峰,等.127名汉族青年口腔唾液一氧化氮含量检测.牙体牙髓牙周病学杂志,2000,10(2)：76-77.
[35]Kankanian AL, Akopov SE.The stimulation of nitric oxide synthesis as a possible protective function of the saliva and its disorder in periodontal diseases.Stomat-ologiia,1996,75(3):19-21.
[36]Takeichi O, Saito I, Okmnoto Y, et al.Lear leukocytes as a mechamism for the development of periodontal lesions.Stomatologiia,1996,75(2):12-14.
[37]Takeichi O, Saito I, Okmnoto Y, et al.Cytokine regulation on the synthesis of nitric oxide in vivo by chronically infected human polymorphonuclear leucocytes. Immunology,1998,93(2):275-280.
[38]许强.一氧化氮及其合成酶在颞下颌关节紊乱病中的作用.国外医学口腔医学分册,2001,28(2)：90-93.
[39]徐芸主译.口腔正畸学—现代原理与技术[M].天津：天津科技翻译出版公司,1997,365-374.
[40]裴秀洁.一氧化氮在口腔正畸学中的研究进展.口腔医学研究,2004,5：558-559.
[41]Chae HJ, Park PK, Chung HT, et al. Nitric oxide is a regulator of bone remodelling.J Pharm Pharmacol,1997,49(5):897-902.
[42]傅民魁.口腔正畸学[M].第4版.北京：人民卫生出版社,2003,165-172.
[43]Shirazi M, Nilforoushan D, Alghasi H, et al.The role of nitric oxide in orthodontic tooth movement in rats. Angle Orthod,2002,72(3):211-215.
[44]裴秀洁,林桂书.大鼠正畸过程牙周组织中NOS三种同形异构体的表达及意义.河北医科大学学报,2005,26(6)：436-439.
[45]尚政军,李金荣,李祖兵,等.口腔鳞状细胞癌中内皮型一氧化氮合酶基因表达的体外研究.中国口腔颌面外科杂志,2003,1(3)：175-178.
[46]杨萍,高文信,魏秀峰,等.口腔癌前病变、口腔鳞癌中诱导型一氧化氮合酶与p53蛋白表达及细胞增殖相互关系的研究.临床口腔医学杂志,2004,20(3)：143-145.
[47]Hilbig H, Franke H, Bidmon H, et al.Nitric oxide synthase isoenzymes during in vitro development of rat neuronal and human non-neuronal cells.Neurosci Lett, 2001,297(1):9-12.
[48]Peunova N, Enikolopov GNitric oxide triggers a switch to growth arrest during differentiation of neuronal cells. Nature,1995,375(6526):68-73.
[49]林绿标,章翔,吴景文,等.小鼠神经干细胞中一氧化氮合成酶的表达.第四军医大学学报,2002,23(23)：2160-2163.
[50]Peunova N, Scheinker V, Cline H, et al.Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain.J Neurosci,2001,21 (22):8809-8818.
[51]Mais A, Klein T, Ullrich V, et al.Prostanoid pattern and iNOS expression during chondrogenic differentiation of human mesenchymal stem cells.J Cell Biochem, 2006,98(4):798-809.
[52]章翔,林绿标,姬西团,等.外源性一氧化氮对小鼠神经干细胞增殖的抑制作用,第四军医大学学报,2004,25(4)：297-300.
[53]林绿标,章翔,姬西团,等.外源性一氧化氮对神经干细胞分化的促进作用.第四军医大学学报,2004,25(4)：301-303.
[54]Wei Pan, Quarles L D, Song LH, et al.Genistein stimulates the osteoblastic differentiation via NO/cGMP in bone marrow culture. J Cell Biochem,2005,94(2): 307-316.
[55]Evans DM, Ralston SH.Nitric oxide and bone.J Bone Miner Res,1996,11(3): 300-305.
[56]金晓明,李雅馨,李金荣,等.外源性转化生长因子β1对口腔鳞状细胞癌细胞系一氧化氮含量的影响.现代口腔医学杂志,2003,17(3)：206-208.