sICAM-1、sTM在抗磷脂综合征自然流产患者中的检测及意义
摘要
目的:通过检测抗磷脂综合征自然流产患者血清可溶性细胞间粘附分子—1(sICAM-1)、可溶性血栓调节蛋白(sTM)的浓度,探讨二者在抗磷脂综合征发病机制中的作用。
方法:
将40例有自然流产史的APS患者作为研究组,33例有自然流产史的非APS患者作为对照Ⅰ组,20例正常无自然流产史的妇女作为对照Ⅱ组。采用酶联免疫吸附试验(ELISA)检测血清ACA、aβ2GPⅠ、sICAM-1、sTM浓度,自动血液流变仪检测血浆粘度。
结果:
1、研究组、对照Ⅰ组、Ⅱ组sICAM-1浓度分别为338.49±14.88ng/ml,259.50±14.17ng/ml,253.51±13.12ng/ml;sTM浓度分别为22.07±2.28ng/ml、14.15±0.90ng/ml、14.06±1.30 ng/ml,研究组sICAM-1、sTM浓度均显著高于对照Ⅰ组及Ⅱ组(P＜0.001),对照Ⅰ组与Ⅱ组比较,差异均无统计学意义(P＞0.05)。
2、研究组ACA阳性患者ACA、sICAM-1、sTM浓度分别为16.27±2.93u/ml,341.45±16.65ng/ml、22.60±2.58 ng/ml,ACA与sICAM-1、sTM浓度的相关系数分别为0.467(P＜0.05)、0.503(P＜0.01),ACA与sICAM-1、sTM浓度均呈正相关。
aβ2GPⅠ阳性患者aβ2GPⅠ、sICAM-1、sTM浓度分别为11.40±1.56 u/ml、343.69±14.67 ng/ml、22.67±2.15 ng/ml,aβ2GPⅠ与sICAM-1、sTM浓度的相关系数分别为0.835(P＜0.001)、0.859(P＜0.001),aβ2GPⅠ与sICAM-1、sTM浓度均呈正相关。
3、研究组sICAM-1与sTM浓度相关系数为0.799(P＜0.001),二者呈正相关。研究组血浆粘度为1.86±0.16 mPa·s,高于对照Ⅰ组及Ⅱ组(P＜0.001)。sICAM-1与血浆粘度的相关系数为0.669(P＜0.001),二者呈正相关。
4、研究组治疗前ACA、aβ2GPⅠ、sICAM-1、sTM浓度、血浆粘度分别为13.08±5.04 u/ml、9.59±8.7 u/ml、338.49±14.88 ng/ml、22.07±2.28ng/ml、1.86±0.16 mPa·s;治疗后各指标分别为6.93±2.10 u/ml、5.04±1.74 u/ml、276.91±7.35 ng/ml、16.13±1.76ng/ml、1.63±0.05mPa·s。治疗后各指标浓度均显著低于治疗前(P＜0.001)。
结论:
1、sICAM-1及sTM与APS有关,可引起血液高凝。
2、sICAM-1及sTM可作为判断APS病情程度的指标。
Objects:To detect the concentration of soluble intercellular adhesion molecule-1(sICAM-1)and soluble thrombomodulin protein (sTM)in patients of antiphospholipid syndrome with spontaneous abortion,research their role of the pathogenesis of antiphospholipid syndrome.
Methods:The serum samples,which were obtained from the study group,composed of 40 APS patients with spontaneous abortion,from the control groupⅠ,composed of 33 non- APS patients with spontaneous abortion,and from the control groupⅡ,composed of 20 normal women, were measured by Enzyme-Linked immunosorbant assay for ACA、aβ2GPⅠ、sICAM-1 and sTM.Plasma viscosity was measured by automatic-hemorheologic machine.
Results:
(1)SICAM-1 concentration of the study group,the control groupⅠandⅡwere 338.49±14.88 ng/ml,259.50±14.17ng/ml and 253.51±13.12ng/ml,sTM concentration in these three groups were 22.07±2.28ng/ml、14.15±0.90ng/ml and 14.06±1.30 ng/ml.The study group demonstrated significantly higher concentration of sICAM-1 and sTM than that of the control groupⅠandⅡ(P＜0.001).Between the control groupⅠandⅡ,there have no significant difference(p＞0.05)
(2)In ACA positive patients of the Study Group,ACA、sICAM-1 and sTM concentration was 16.27±2.93u/ml、341.45±16.65ng/ml and 22.60±2.58 ng/ml.The Pearson correlation coefficient between ACA and sICAM-1、sTM was 0.467(P＜0.05)、0.503 (P＜0.01).The relationship of ACA and sICAM-1、sTM is positive correlation.
In aβ2GPⅠpositive patients,aβ2GPⅠ、sICAM-1 and sTM concentration was 11.40±1.56 u/ml、343.69±14.67 ng/ml and 22.67±2.15 ng/ml.The Pearson correlation coefficient between aβ2GPⅠand sICAM-1、sTM was 0.835(P＜0.001)、0.859(P＜0.001)The relationship of aβ2GPⅠand sICAM-1、sTM is positive correlation.
(3)In the study group,the Pearson correlation coefficient between sICAM-1 and sTM concentration was 0.799(P＜0.001),the two are positive correlated.The plasma viscosity was 1.86±0.16 mPa-s,which demonstrated higher concentration than that of the control groupⅠandⅡ(P＜0.001).The Pearson correlation coefficient between sICAM-1 and plasma viscosity was 0.669(P＜0.001),the two are positive correlated.
(4)In the study group,ACA、aβ2GPⅠ、sICAM-1、sTM concentration and plasma viscosity of before treatment was 13.08±5.04 u/ml、9.59±8.7 u/ml、338.49±14.88 ng/ml、22.07±2.28ng/ml、1.86±0.16 mPa·s;,and of after treatment was 6.93±2.10 u/ml、5.04±1.74 u/ml、276.91±7.35 ng/ml、16.13±1.76 ng/ml、1.63±0.05mPa·s.The group after treatment demonstrated significantly lower concentration of each than the the group before treatment(P＜0.001).
Conclusions:
(1)SICAM-1 and sTM correlate with APS.They may cause blood hypercoagulability.
(2)SICAM-1 and sTM can be used as a severity index of APS.
方法:
将40例有自然流产史的APS患者作为研究组,33例有自然流产史的非APS患者作为对照Ⅰ组,20例正常无自然流产史的妇女作为对照Ⅱ组。采用酶联免疫吸附试验(ELISA)检测血清ACA、aβ2GPⅠ、sICAM-1、sTM浓度,自动血液流变仪检测血浆粘度。
结果:
1、研究组、对照Ⅰ组、Ⅱ组sICAM-1浓度分别为338.49±14.88ng/ml,259.50±14.17ng/ml,253.51±13.12ng/ml;sTM浓度分别为22.07±2.28ng/ml、14.15±0.90ng/ml、14.06±1.30 ng/ml,研究组sICAM-1、sTM浓度均显著高于对照Ⅰ组及Ⅱ组(P＜0.001),对照Ⅰ组与Ⅱ组比较,差异均无统计学意义(P＞0.05)。
2、研究组ACA阳性患者ACA、sICAM-1、sTM浓度分别为16.27±2.93u/ml,341.45±16.65ng/ml、22.60±2.58 ng/ml,ACA与sICAM-1、sTM浓度的相关系数分别为0.467(P＜0.05)、0.503(P＜0.01),ACA与sICAM-1、sTM浓度均呈正相关。
aβ2GPⅠ阳性患者aβ2GPⅠ、sICAM-1、sTM浓度分别为11.40±1.56 u/ml、343.69±14.67 ng/ml、22.67±2.15 ng/ml,aβ2GPⅠ与sICAM-1、sTM浓度的相关系数分别为0.835(P＜0.001)、0.859(P＜0.001),aβ2GPⅠ与sICAM-1、sTM浓度均呈正相关。
3、研究组sICAM-1与sTM浓度相关系数为0.799(P＜0.001),二者呈正相关。研究组血浆粘度为1.86±0.16 mPa·s,高于对照Ⅰ组及Ⅱ组(P＜0.001)。sICAM-1与血浆粘度的相关系数为0.669(P＜0.001),二者呈正相关。
4、研究组治疗前ACA、aβ2GPⅠ、sICAM-1、sTM浓度、血浆粘度分别为13.08±5.04 u/ml、9.59±8.7 u/ml、338.49±14.88 ng/ml、22.07±2.28ng/ml、1.86±0.16 mPa·s;治疗后各指标分别为6.93±2.10 u/ml、5.04±1.74 u/ml、276.91±7.35 ng/ml、16.13±1.76ng/ml、1.63±0.05mPa·s。治疗后各指标浓度均显著低于治疗前(P＜0.001)。
结论:
1、sICAM-1及sTM与APS有关,可引起血液高凝。
2、sICAM-1及sTM可作为判断APS病情程度的指标。
Objects:To detect the concentration of soluble intercellular adhesion molecule-1(sICAM-1)and soluble thrombomodulin protein (sTM)in patients of antiphospholipid syndrome with spontaneous abortion,research their role of the pathogenesis of antiphospholipid syndrome.
Methods:The serum samples,which were obtained from the study group,composed of 40 APS patients with spontaneous abortion,from the control groupⅠ,composed of 33 non- APS patients with spontaneous abortion,and from the control groupⅡ,composed of 20 normal women, were measured by Enzyme-Linked immunosorbant assay for ACA、aβ2GPⅠ、sICAM-1 and sTM.Plasma viscosity was measured by automatic-hemorheologic machine.
Results:
(1)SICAM-1 concentration of the study group,the control groupⅠandⅡwere 338.49±14.88 ng/ml,259.50±14.17ng/ml and 253.51±13.12ng/ml,sTM concentration in these three groups were 22.07±2.28ng/ml、14.15±0.90ng/ml and 14.06±1.30 ng/ml.The study group demonstrated significantly higher concentration of sICAM-1 and sTM than that of the control groupⅠandⅡ(P＜0.001).Between the control groupⅠandⅡ,there have no significant difference(p＞0.05)
(2)In ACA positive patients of the Study Group,ACA、sICAM-1 and sTM concentration was 16.27±2.93u/ml、341.45±16.65ng/ml and 22.60±2.58 ng/ml.The Pearson correlation coefficient between ACA and sICAM-1、sTM was 0.467(P＜0.05)、0.503 (P＜0.01).The relationship of ACA and sICAM-1、sTM is positive correlation.
In aβ2GPⅠpositive patients,aβ2GPⅠ、sICAM-1 and sTM concentration was 11.40±1.56 u/ml、343.69±14.67 ng/ml and 22.67±2.15 ng/ml.The Pearson correlation coefficient between aβ2GPⅠand sICAM-1、sTM was 0.835(P＜0.001)、0.859(P＜0.001)The relationship of aβ2GPⅠand sICAM-1、sTM is positive correlation.
(3)In the study group,the Pearson correlation coefficient between sICAM-1 and sTM concentration was 0.799(P＜0.001),the two are positive correlated.The plasma viscosity was 1.86±0.16 mPa-s,which demonstrated higher concentration than that of the control groupⅠandⅡ(P＜0.001).The Pearson correlation coefficient between sICAM-1 and plasma viscosity was 0.669(P＜0.001),the two are positive correlated.
(4)In the study group,ACA、aβ2GPⅠ、sICAM-1、sTM concentration and plasma viscosity of before treatment was 13.08±5.04 u/ml、9.59±8.7 u/ml、338.49±14.88 ng/ml、22.07±2.28ng/ml、1.86±0.16 mPa·s;,and of after treatment was 6.93±2.10 u/ml、5.04±1.74 u/ml、276.91±7.35 ng/ml、16.13±1.76 ng/ml、1.63±0.05mPa·s.The group after treatment demonstrated significantly lower concentration of each than the the group before treatment(P＜0.001).
Conclusions:
(1)SICAM-1 and sTM correlate with APS.They may cause blood hypercoagulability.
(2)SICAM-1 and sTM can be used as a severity index of APS.
引文
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[2]Simantov E,Lasala J,Lo SK,et al.Activation of cultured vascular endothelial cells by antiphospholipid antibodies[J].J Clin Invest,1995,96:2211-2219
[3]Del Papa N,Guidali L,Spatola L,et al.Relationship between anti-phospholipid and anti-endothelial cell antibodies:β_2 glycoprotein I mediates the antibody binding to endothelial membranes and induces the expression of adhesion molecules[J].Clin Exp Rheumatol.1995,13:179-185
[4]Silvia S.Pierangeli,Ricardo G,et al.Thrombogenic Effects of Antiphospholipid Antibodies Are Mediated by Intercellular Cell Adhesion Molecule-1,Vascular Cell Adhesion Molecule-1,and P-Selectin[J].Circulation Research,2001,88:245
[5]Kotajima L,Aotsuka S,Sato T.Clinical significance of serum thrombomodulin levels in patients with systemic rheumatic diseases.Clin Exp Rheumatol 1997;15:59-65
[6]Karmochkine M,Boffa MC,Piette JC,Cacoub P,Wechsler B,Godeau P,et al.Increase in plasma thrombomodulin in lupus erythematosus with antiphospholipid antibodies.Blood 1992;79:837-838
[7]López-Lira F,Rosales-León L,Martínez VM,et al.The role of beta2-glycoprotein I(beta2GPI)in the activation of plasminogen[J].Biochim Biophys Acta,2006,1764(4):815-823
[8]Jang Y,Lincoff AM,plow EF,et al.Cell adhesion molecules in coronary artery disease,TAcc,1 994,24(7):1591-1601
[9]Szekanecz Z,Shah MR,Pearce WH· Intercellular adhesion molecule- 1(ICAM-1)expression and soluble ICAM-1(sICAM-1)production by cytokine activated human aortic endothelial cells:a possible role for ICAM-1 and sICAM-1 in a therosclerotic aortic aneurysms,Clin Exp Immuno,1994,98:337-343
[10]Lasky LA,Selections:interpreters of cell specific carbohydrate information,Science,1992,258:964
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