培养条件对马铃薯试管苗生长及其结薯性能的影响
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摘要
为探讨不同品种、不同光照强度和不同苗龄对试管结薯的影响,本试验设置了三个方面的研究内容,研究结果如下:
1、培养筛选壮苗培养基研究
以西洋为试验材料,采用正交试验设计,研究了蔗糖和外源激素(6-BA、B_9、NM)对脱毒试管苗生长状况的影响。研究结果表明,对于试管苗的不同指标,其最优组合不尽相同。有利于株高的组合:3%蔗糖+0.01 mg.L~(-1)6-BA+20 mg.L~(-1)B9+0.1 mg.L~(-1)NAA;有利于茎粗的组合:12%蔗糖+1mg.L~(-1)6-BA+20 mg.L~(-1)B9+0.02mg.L~(-1)NAA;有利于茎节数的组合:3%蔗糖+0.1 mg.L~(-1)6-BA+35 mg.L~(-1)B9+0.1 mg.L~(-1)NAA;有利于叶片数的组合:3%蔗糖+0.1mg.L~(-1)6-BA+20 mg.L~(-1)B9+0.02 mg.L~(-1)NAA。
2、光照强度对试管苗壮苗的研究
以渝薯1号、大西洋、鄂薯3号为材料,采用光照强度、品种、赤霉素三因素进行裂区设计,考察不同光照强度和不同品种对脱毒试管苗生长状况的影响,研究结果表明,即不遮光(光照强度在3000Lux)、不加赤霉素条件下的脱毒试管苗的长势显著优于遮光的脱毒试管苗。
3、光照强度对试管薯诱导研究
以渝薯1号、大西洋、鄂薯3号为材料,以前试验为基础,壮苗培养一个月后,转入试管薯诱导培养,采用光照强度、苗质、品种三因素进行裂区设计,研究不同光照强、不同品种、不同苗质下的某些生理性状的差异和试管结薯能力的差异。研究结果表明:黑暗条件下、接种苗龄5-7片叶时,其试管薯的平均薯径、结薯数、试管薯产量均显著高于其他处理。即黑暗条件下、接种苗龄为5-7片叶时,可以提高试管薯产量。
To probe into the effect of different potato varieties,light intensity and plantlet age on microtuberization,three aspects were investigated,and the results were as follows.
1.Study on screening of culture medium for stringing plantlet
Potato(Solanum tuberosum L.) cv.Atlantic being as the trial material,the experiment through orthogonalty design researched the effects of sucrose and exogenous hormones(6-BA, B_9,and NAA) on growth of virus free plantlet in vitro.The results suggested that,for different traits of plantlet,the optimum combination was not same.As for the plantlet height,it was the best combination that MS was supplemented with 3%sucrose,0.01mg/L 6-BA,20 mg/L B_9 and 0.1 mg/L NAA.As for the plantlet stem diameter,it was the best combination that MS was supplemented with 12%sucrose,0.1mg/L 6-BA,20 mg/L B_9 and 0.02 mg/L NAA.As for the plantlet stem nodes,it was the best combination that MS was supplemented with 3% sucrose,0.1mg/L 6-BA,35 mg/L B_9 and 0.1 mg/L NAA.And as for the plantlet leaves,the best was the MS supplemented with 3%sucrose,0.1mg/L 6-BA,20 mg/L B_9 and 0.02 mg/L NAA.
2.Study on effect of lighting intensity on plantlet in vitro
Potato cv.Yushu-1,Atlantic,and Eshu-3 as materials were used in the experiment about lighting intensity,variety and gibberellin,and it was designed through crack design to investigate the effect of different lighting intensity and gibberellin concentration on plantlet growth of different potato varieties.The results indicated that the plantlet growing vigor of plantlets on the circumstances of 3000Lux lighting markedly outwent the plantlets under the weak lighting intensity.
3.Study on the inducement of lighting intensity for tuberization of potato in vitro
Potato cv.Yushu-1,Atlantic,and Eshu-3 being as the trial materials,the culture medium for strong plantlet was substituted with that for inducement after the plantlets was cultured for a month.The trial was carried out with crack design concerned with lighting,plantlet quality and variety to study the difference of physiological traits and microtuberization at the different lighting intensity,variety and plantlet quality.The result suggested that under the circumstances of darkness and at the age of plantlet with 5-7 leaves,the microtuber diameter, tuberizing rate and microtuber yield were markedly higher than other transactions.
1、培养筛选壮苗培养基研究
以西洋为试验材料,采用正交试验设计,研究了蔗糖和外源激素(6-BA、B_9、NM)对脱毒试管苗生长状况的影响。研究结果表明,对于试管苗的不同指标,其最优组合不尽相同。有利于株高的组合:3%蔗糖+0.01 mg.L~(-1)6-BA+20 mg.L~(-1)B9+0.1 mg.L~(-1)NAA;有利于茎粗的组合:12%蔗糖+1mg.L~(-1)6-BA+20 mg.L~(-1)B9+0.02mg.L~(-1)NAA;有利于茎节数的组合:3%蔗糖+0.1 mg.L~(-1)6-BA+35 mg.L~(-1)B9+0.1 mg.L~(-1)NAA;有利于叶片数的组合:3%蔗糖+0.1mg.L~(-1)6-BA+20 mg.L~(-1)B9+0.02 mg.L~(-1)NAA。
2、光照强度对试管苗壮苗的研究
以渝薯1号、大西洋、鄂薯3号为材料,采用光照强度、品种、赤霉素三因素进行裂区设计,考察不同光照强度和不同品种对脱毒试管苗生长状况的影响,研究结果表明,即不遮光(光照强度在3000Lux)、不加赤霉素条件下的脱毒试管苗的长势显著优于遮光的脱毒试管苗。
3、光照强度对试管薯诱导研究
以渝薯1号、大西洋、鄂薯3号为材料,以前试验为基础,壮苗培养一个月后,转入试管薯诱导培养,采用光照强度、苗质、品种三因素进行裂区设计,研究不同光照强、不同品种、不同苗质下的某些生理性状的差异和试管结薯能力的差异。研究结果表明:黑暗条件下、接种苗龄5-7片叶时,其试管薯的平均薯径、结薯数、试管薯产量均显著高于其他处理。即黑暗条件下、接种苗龄为5-7片叶时,可以提高试管薯产量。
To probe into the effect of different potato varieties,light intensity and plantlet age on microtuberization,three aspects were investigated,and the results were as follows.
1.Study on screening of culture medium for stringing plantlet
Potato(Solanum tuberosum L.) cv.Atlantic being as the trial material,the experiment through orthogonalty design researched the effects of sucrose and exogenous hormones(6-BA, B_9,and NAA) on growth of virus free plantlet in vitro.The results suggested that,for different traits of plantlet,the optimum combination was not same.As for the plantlet height,it was the best combination that MS was supplemented with 3%sucrose,0.01mg/L 6-BA,20 mg/L B_9 and 0.1 mg/L NAA.As for the plantlet stem diameter,it was the best combination that MS was supplemented with 12%sucrose,0.1mg/L 6-BA,20 mg/L B_9 and 0.02 mg/L NAA.As for the plantlet stem nodes,it was the best combination that MS was supplemented with 3% sucrose,0.1mg/L 6-BA,35 mg/L B_9 and 0.1 mg/L NAA.And as for the plantlet leaves,the best was the MS supplemented with 3%sucrose,0.1mg/L 6-BA,20 mg/L B_9 and 0.02 mg/L NAA.
2.Study on effect of lighting intensity on plantlet in vitro
Potato cv.Yushu-1,Atlantic,and Eshu-3 as materials were used in the experiment about lighting intensity,variety and gibberellin,and it was designed through crack design to investigate the effect of different lighting intensity and gibberellin concentration on plantlet growth of different potato varieties.The results indicated that the plantlet growing vigor of plantlets on the circumstances of 3000Lux lighting markedly outwent the plantlets under the weak lighting intensity.
3.Study on the inducement of lighting intensity for tuberization of potato in vitro
Potato cv.Yushu-1,Atlantic,and Eshu-3 being as the trial materials,the culture medium for strong plantlet was substituted with that for inducement after the plantlets was cultured for a month.The trial was carried out with crack design concerned with lighting,plantlet quality and variety to study the difference of physiological traits and microtuberization at the different lighting intensity,variety and plantlet quality.The result suggested that under the circumstances of darkness and at the age of plantlet with 5-7 leaves,the microtuber diameter, tuberizing rate and microtuber yield were markedly higher than other transactions.
引文
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