尿畅舒胶囊对女性绝经后尿道综合征的临床与实验研究
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摘要
目的:本课题拟通过对尿畅舒胶囊的临床疗效观察和实验动物子宫重量、血清雌二醇及尿道平滑肌的影响,为探讨尿畅舒胶囊治疗绝经后尿道综合征提供可能的作用机制。
方法:1.2005年3月至2005年9月在成都中医药大学附属医院就诊的绝境后尿道综合征的病人40例随机分为治疗组和阳性组,分别予以尿畅舒胶囊和尼尔雌醇片口服4个月后临床疗效观察。2.将幼年昆明雌性小鼠60只随机分成5组:空白组、阳性对照组、尿畅舒高、中和低剂量组,分别对高、中、低剂量组,选用尼尔雌醇片、尿畅舒胶囊混悬液灌胃,均连续给药7天.末次给药24小时后处死,测子宫重量。3.将SD雌性大鼠80只筛选出动情期大鼠20只作为空白组,将剩余的造模后筛选出间情期大鼠50只随机分为5组:模型组、阳性对照组、尿畅舒高、中和低剂量组。分选用尿畅舒胶囊和戊酸雌二醇片的混悬液灌胃,连续给药10天。空白组筛选出动情前期的大鼠10只和其余各组50只于末次给药后1小时,由眼眶静脉丛取血测定血清中雌二醇的浓度。将各组大鼠处死取尿道组织HE染色和光镜观测其尿道平滑肌纤维的改变情况。
结果:1.尿畅舒胶囊能缓解患者的排尿症状。2.尿畅舒高剂量组对幼年小鼠子宫重量有增加作用。3.尿畅舒高剂量组能显著升高去势大鼠血清中的雌二醇浓度,与模型组比较差异有统计学意义4.尿畅舒高剂量组对去势大鼠尿道平滑肌纤维可能促进其修复作用。
结论:尿畅舒胶囊升高实验性雌鼠的雌激素水平,但作用较为缓和;尿畅舒胶囊缓解排尿症状可能作用于尿道粘膜,促进平滑肌纤维的修复。
Objective: This trial is to observe the influence of the capsule called Niaochangshu on womb' s weight、the Estradiol in blood serum and urethra smooth muscle of female rat, as well as to explore its possible role in the mechanism further.
Methods: 1.40 post-menopause urethral syndrome female of Chengdu University of TCM from March 2005 to September 2005 were divided into 2 groups randomly: treated group and control group, with were taken Niaochangshu collocyst and Nilestriol pill respectively, and after a month observe the clinic syndrome. 2. The sixty childhood female rats of the Kunming special will be divided into five groups: the blank group, the masculine group, and the highest dose group, the middle dose group, the lowest dose group of the Niaochangshu. Take Nilestriol pills and theNiaochangshu collocyst for intragastric administration. Then give theabove rat experimental medication per day a week. Last sacrifice the ratsand measure the wombs' weight. 3. From the eighty SD female rats sieve twenty estrus rats for blank group. The rest rats will be modeled and sieved fifty diestrus rats, which will be divided into five groups:the modle group, the masculine group, And the Niaochangshu highest dose group, middle dose group, lowest dose group. Use Estradiol Valerate pills and Niaochangshu collocystis for intragastric administration. Then give the above rat experimental medication per day in ten days. Then from the twenty rats sieves the proestrus rats as a blank group. Last from the fossa orbitalis of rats measure the Estradiol in blood serum. Ending sacrifice the rats and take urethra tissue, following HE staining and using the light microscope observe the urethra smooth muscle.
Results: 1. Niaochangshu capsule can relieve the patient of urination syndrome. 2.Niaochangshu high dose group increase childhood female rat 's womb's weight. 3. Niaochangshu high dose group raise the estradiol in blood serum of castration rats. There is a statistical difference between the Niaochangshu high dose group and the model group. Niaochangshu high dose group may has effects on urethra smooth muscle and promote it reparation of castration rats.
Conclusion: Niaochangshu collocyst boost the estrogen level of the female rats in experiment. Niaochangshu collocyst which can relieve the patient of urination syndrome has effects Mucous membrane of urethra and promote smooth muscle fiber reparation.
方法:1.2005年3月至2005年9月在成都中医药大学附属医院就诊的绝境后尿道综合征的病人40例随机分为治疗组和阳性组,分别予以尿畅舒胶囊和尼尔雌醇片口服4个月后临床疗效观察。2.将幼年昆明雌性小鼠60只随机分成5组:空白组、阳性对照组、尿畅舒高、中和低剂量组,分别对高、中、低剂量组,选用尼尔雌醇片、尿畅舒胶囊混悬液灌胃,均连续给药7天.末次给药24小时后处死,测子宫重量。3.将SD雌性大鼠80只筛选出动情期大鼠20只作为空白组,将剩余的造模后筛选出间情期大鼠50只随机分为5组:模型组、阳性对照组、尿畅舒高、中和低剂量组。分选用尿畅舒胶囊和戊酸雌二醇片的混悬液灌胃,连续给药10天。空白组筛选出动情前期的大鼠10只和其余各组50只于末次给药后1小时,由眼眶静脉丛取血测定血清中雌二醇的浓度。将各组大鼠处死取尿道组织HE染色和光镜观测其尿道平滑肌纤维的改变情况。
结果:1.尿畅舒胶囊能缓解患者的排尿症状。2.尿畅舒高剂量组对幼年小鼠子宫重量有增加作用。3.尿畅舒高剂量组能显著升高去势大鼠血清中的雌二醇浓度,与模型组比较差异有统计学意义4.尿畅舒高剂量组对去势大鼠尿道平滑肌纤维可能促进其修复作用。
结论:尿畅舒胶囊升高实验性雌鼠的雌激素水平,但作用较为缓和;尿畅舒胶囊缓解排尿症状可能作用于尿道粘膜,促进平滑肌纤维的修复。
Objective: This trial is to observe the influence of the capsule called Niaochangshu on womb' s weight、the Estradiol in blood serum and urethra smooth muscle of female rat, as well as to explore its possible role in the mechanism further.
Methods: 1.40 post-menopause urethral syndrome female of Chengdu University of TCM from March 2005 to September 2005 were divided into 2 groups randomly: treated group and control group, with were taken Niaochangshu collocyst and Nilestriol pill respectively, and after a month observe the clinic syndrome. 2. The sixty childhood female rats of the Kunming special will be divided into five groups: the blank group, the masculine group, and the highest dose group, the middle dose group, the lowest dose group of the Niaochangshu. Take Nilestriol pills and theNiaochangshu collocyst for intragastric administration. Then give theabove rat experimental medication per day a week. Last sacrifice the ratsand measure the wombs' weight. 3. From the eighty SD female rats sieve twenty estrus rats for blank group. The rest rats will be modeled and sieved fifty diestrus rats, which will be divided into five groups:the modle group, the masculine group, And the Niaochangshu highest dose group, middle dose group, lowest dose group. Use Estradiol Valerate pills and Niaochangshu collocystis for intragastric administration. Then give the above rat experimental medication per day in ten days. Then from the twenty rats sieves the proestrus rats as a blank group. Last from the fossa orbitalis of rats measure the Estradiol in blood serum. Ending sacrifice the rats and take urethra tissue, following HE staining and using the light microscope observe the urethra smooth muscle.
Results: 1. Niaochangshu capsule can relieve the patient of urination syndrome. 2.Niaochangshu high dose group increase childhood female rat 's womb's weight. 3. Niaochangshu high dose group raise the estradiol in blood serum of castration rats. There is a statistical difference between the Niaochangshu high dose group and the model group. Niaochangshu high dose group may has effects on urethra smooth muscle and promote it reparation of castration rats.
Conclusion: Niaochangshu collocyst boost the estrogen level of the female rats in experiment. Niaochangshu collocyst which can relieve the patient of urination syndrome has effects Mucous membrane of urethra and promote smooth muscle fiber reparation.
引文
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[5]卢子杰,黄树纲.女性尿道综合征病机与辨证分型相关性的临床研究.新中医,2001,33(7):25-26
[6]李建生,赵巧荣,晋耐砚.“补肾缓急方”治疗中老年女性非微生物性尿道综合征21例.河南中医药学刊,1995,10(3):55-56
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[2]中国妇产学专家经验文集.沈阳出版社,1994.468
[3]吴阶平.泌尿外科.济南:山东科学技术出版社,1993.778
[4]邓小虹.更年期老年妇女疾病.载顾美蛟主编《现代妇产科学》第一版,北京人民军医出版社,2002,1218-1244
[5]章婷婷,刚君,方小玲.雌激素替代疗法的利与弊.医学与哲学,2006,27(1):46-48
[6]张亚大,卢子杰.女性尿道综合征中医证候学临床研究附265例临床资料分析[J].中华实用中西医杂志,2001,1(14),(7.19):1655.
[7]陈贵延,《最新国内外疾病诊疗标准》[M].北京:学苑出版社,1991
[8]徐叔云,主编:药理实验方法学.北京:人民卫生出版社.1991.1293
[9]许振燕,王钦茂.中西医结合治疗非感染性尿道综合征临床观察.实用中西医结合临床,2002,2(2):11-12
[10]卢子杰,黄树纲.女性尿道综合征病机与辨证分型相关性的临床研究.新中医,2001,33(7):25-26
[11]李建生,赵巧荣,晋耐砚.“补肾缓急方”治疗中老年女性非微生物性尿道综合征21例.河南中医药学刊,1995,10(3):55-56
[12]张殿延,刘珍.绝经后妇女尿道综合征雌激素补充治疗的临床研究.中国综合临床,2001,17(8):631-632
[13]康亚波,段源辉.尼尔雌醇治疗老年尿道综合征32例疗效观察.中国医师杂志,2000,增刊:66
[14]云曼丽,陈裕盛.尿道综合征诊断及治疗体会-附15例报告.湖南医学院学报,1998,1(4):23-24
[15]吴阶平.泌尿外科学.山东:山东科学技术出版社,2004,1347-1402
[16]卢子杰,张亚大.女性尿道综合征中医辨证分型临床研究.安徽中医临床杂志,2001,13(2):106-107
[17]Bkies AL,Lombard C,Strauss BJG,Wilcox G,Burger HG.Dietary flour supplementation decreases post-menopausal Effect of soy and wheat[J].Maturitas,1995,21:189-195
[18]Yan Z,Tong TS,Joan EC,Patricia AM,Suzanne H.Daidzein and genistein glucuronides in vitro are weakly estrogenic and activate human natural killer cells at nutritionally relevant concentrations[J].J Nutr,1999;129(2):399-405
[19]Scheiber MD,Rebar RW.Isoflavones and postmenopausal bone health:A viable alternative to estrogen therapy[J].Menopause,1999,6(3):233-241
[20]Eden J.Phytoestrogens and the menopause[J].Baillieres Clin Endocrinol Metab,1998,12(4)4581-587
[21]Kass-Annese B.A lternative therapies for menopause [J].Clin Obstet Gynecol,2000,43(1):162-183
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