蛇根木中两个重要酶的表达，纯化，结晶，复合物三维结构表征和底物扩展研究
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摘要
蛇根木(Rauvolfia serpentina Benth. Ex Kurz)为夹竹桃科萝芙木属药用植物,富含生物碱类化合物,其主要活性成分为阿吗灵、阿吗碱、利血平和熊果苷等。其有降血压、抗心律失常、退热、抗癫、消炎及治虫蛇咬伤等功效。对其次生代谢产物生物合成途径关键酶的研究是进一步开发利用该药用植物、调控生物代谢方向乃至体外生物-化学合成活性化合物的基础。本论文对参与蛇根木生物碱阿吗灵生物合成途径的异胡豆苷合成酶(STR1)进行了底物扩展及结构生物学研究。除了基于结构信息的应用研究外,本论文还对蛇根木中熊果苷合成酶(AS)的结晶条件进行了系统的考察。研究主要涉及酶的表达、纯化、结晶条件筛选、三维结构表征及催化机理研究等。
为进一步扩展STR1可接受底物数量,拓展其在生物-化学合成应用领域的研究口径。本论文在基于酶STR1结构信息的基础上,考察STR1对理性设计候选底物的活性。首次发现了通过STR1酶可催化合成具有新型哌嗪吲哚骨架核心的异胡豆苷类似物。并通过晶体浸泡和共结晶相结合的方法获得了STR1与各配体的复合物晶体X-射线衍射结构。详细探讨了STR1与各配体间的构效关系。阐明了各候选底物影响该酶活性的关键结构及构象因素。为理性底物扩展研究提供了极好的事实范例和结构信息基础。
作为底物适应性出色的糖基转移酶,AS的活性位点、催化机理及多底物适应性机理不明。因此对AS酶进行晶体X-射线衍射三维结构研究显得尤为重要。本论文对酶AS的表达体系构建、表达、纯化及气相扩散法结晶条件筛选进行了系统的研究。通过配体诱导蛋白稳定性和高通量筛选方法获得酶AS晶体。为酶AS三维结构的获得及理性生物工程改造研究奠定了坚实的基础。
Rauvolfia serpentina Benth-. Ex Kurz (Apocynaceae) has been used as medicinal plant in India for thousands of years, mainly treating hypertension, antiarrhythmia, fevers, insanity, anti-inflammation and snake bites. The major active constituents are ajmaline, ajmalicine, reserpine and arbutin. Research on key enzymes involved in biosynthesis of activity compounds in plant allows better understanding and utilizing of Rauvolfia, steering the pathway into the direction of a desired product by pathway blocking and gene manipulation, in vitro chemoenzymatic synthesis of complex skeleton compounds. This thesis is carried out for rational substrate exploring and structure biology research of Rauvolfia enzyme Strictosidine Synthase (STRl). Besides above, The expression, purification and crystallization condition of Arbutin Synthesis (AS) in Rauvolfia is also investigated in detail.
For exploring accepted novel substrates of STRl, expanding the way to various chemoenzymatic strategies, Novel substrates were rational designed basing structure information. A novel function of STRl has been detected which allows for the first time a simple enzymatic synthesis of strictosidine analogue harboring the piperazino[1,2-a]indole scaffold. Insight into the relationship between activity and conformation of substrates in STRl is provided by using state of art X-ray crystal analysis and modeling of STRl ligand complexes. Substituent effect of accepted compounds on activity loss is also discussed here. The results provide example and structural information for further STRl substrate expanding and activity mechanism research.
In order to clarify the3D-structure, activity center and mechanism of reaction catalyzed by AS which perform extraordinary in substrate acceptance abitity, expression, purification and crystallization condition of AS were investigated in detail. Crystals of AS were got by regular high-throughput screening and further optimization method, which provides first example of crystal stability induced by ligand binding and opens the gate for preliminary X-ray analysis of AS.
为进一步扩展STR1可接受底物数量,拓展其在生物-化学合成应用领域的研究口径。本论文在基于酶STR1结构信息的基础上,考察STR1对理性设计候选底物的活性。首次发现了通过STR1酶可催化合成具有新型哌嗪吲哚骨架核心的异胡豆苷类似物。并通过晶体浸泡和共结晶相结合的方法获得了STR1与各配体的复合物晶体X-射线衍射结构。详细探讨了STR1与各配体间的构效关系。阐明了各候选底物影响该酶活性的关键结构及构象因素。为理性底物扩展研究提供了极好的事实范例和结构信息基础。
作为底物适应性出色的糖基转移酶,AS的活性位点、催化机理及多底物适应性机理不明。因此对AS酶进行晶体X-射线衍射三维结构研究显得尤为重要。本论文对酶AS的表达体系构建、表达、纯化及气相扩散法结晶条件筛选进行了系统的研究。通过配体诱导蛋白稳定性和高通量筛选方法获得酶AS晶体。为酶AS三维结构的获得及理性生物工程改造研究奠定了坚实的基础。
Rauvolfia serpentina Benth-. Ex Kurz (Apocynaceae) has been used as medicinal plant in India for thousands of years, mainly treating hypertension, antiarrhythmia, fevers, insanity, anti-inflammation and snake bites. The major active constituents are ajmaline, ajmalicine, reserpine and arbutin. Research on key enzymes involved in biosynthesis of activity compounds in plant allows better understanding and utilizing of Rauvolfia, steering the pathway into the direction of a desired product by pathway blocking and gene manipulation, in vitro chemoenzymatic synthesis of complex skeleton compounds. This thesis is carried out for rational substrate exploring and structure biology research of Rauvolfia enzyme Strictosidine Synthase (STRl). Besides above, The expression, purification and crystallization condition of Arbutin Synthesis (AS) in Rauvolfia is also investigated in detail.
For exploring accepted novel substrates of STRl, expanding the way to various chemoenzymatic strategies, Novel substrates were rational designed basing structure information. A novel function of STRl has been detected which allows for the first time a simple enzymatic synthesis of strictosidine analogue harboring the piperazino[1,2-a]indole scaffold. Insight into the relationship between activity and conformation of substrates in STRl is provided by using state of art X-ray crystal analysis and modeling of STRl ligand complexes. Substituent effect of accepted compounds on activity loss is also discussed here. The results provide example and structural information for further STRl substrate expanding and activity mechanism research.
In order to clarify the3D-structure, activity center and mechanism of reaction catalyzed by AS which perform extraordinary in substrate acceptance abitity, expression, purification and crystallization condition of AS were investigated in detail. Crystals of AS were got by regular high-throughput screening and further optimization method, which provides first example of crystal stability induced by ligand binding and opens the gate for preliminary X-ray analysis of AS.
引文
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