山羊痘病毒某些生物学特性及其主要结构蛋白P32基因的研究
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摘要
山羊痘是由羊痘病毒属(Capripoxvirus)山羊痘病毒(Goat poxvirus,GPV)引起的一种高度接触性传染病,临床上以体温升高,全身皮肤、呼吸道和消化道黏膜出现痘疹为主要特征。本病被世界动物卫生组织(OIE)列为A类重大动物传染病,我国也将其列为一类动物疫病。2002年10月以来,贵州省羊群中首次暴发疑似山羊痘病例,并迅速波及到8个养羊较为集中的地区,对我省养羊业的健康发展构成了巨大的威胁。为了防制本病,本研究对我省两株山羊痘病毒分离株进行了某些生物学特性及其主要结构蛋白P32基因进行了详细研究。
将贵州省山羊痘病毒分离株GPV-LD和GPV-QL接种Vero-E6、BHK-21细胞,3～7d感染细胞显现圆缩、聚集成簇等细胞病变效应;以GPV荧光抗体对感染细胞飞片染色,在细胞浆中检测到特异性的黄绿色荧光;取感染细胞超薄切片进行电子显微镜观察,细胞浆中发现大量成熟和未成熟的痘病毒颗粒;将感染细胞培养物提取总DNA样本,采用针对P32结构蛋白基因和ITR非编码区基因的两对引物进行PCR鉴定,分别扩增出969bp和289bp的DNA片段,对目的DNA片段的测序结果证实感染细胞培养物中存在GPV特异性的病原核酸;采用2000TCID_(50)的病毒感染细胞培养物通过皮下接种3月龄健康山羊,在14～45d成功复制出与山羊痘自然病例相类似的临床症状和剖检病变,并从感染组织材料中回收到接种的病毒。
对GPV结构蛋白P32基因和非编码区ITR基因的两对引物进行比较,以期开展临床山羊痘病例的定性PCR检测。研究结果表明两对引物具有羊痘病毒属特异性,不与副痘病毒属羊传染性脓疱病毒、禽痘病毒属鸡痘病毒、健康山羊皮肤样本发生交叉反应。贵州分离毒P32基因和ITR基因与国内外参考毒株的核苷酸同源性分别达99.5%～100%和100%;PCR最小检出量分别为19.06ng和24.40ng。为此,ITR基因引物更适合于临床山羊痘病例的诊断,而P32基因则有利于病毒囊膜表面蛋白的深入研究。
根据GPV参考毒株的全基因序列,针对gp064基因分别设计与合成了TaqMan-MGB探针和引物,应用实时荧光PCR方法开展对山羊痘病例的定量检测。一般PCR方法只能检测患羊出现痘疹病变的皮肤和黏膜材料,而FQ-PCR方法则能够从患羊鼻腔拭子、血液样本、皮肤、肺、胃、淋巴结等组织材料中检出GPV病原核酸。按照本试验构建的标准曲线,TaqMan-MGB-FQ-PCR方法的检测极限为0.1TCID_(50)的病毒量。检测结果表明在人工复制山羊痘病例中,不同组织的含毒量呈现皮肤＞瘤胃＞肺＞淋巴结的趋势;接种山羊从第9～15d形成病毒血症,持续时间较短;鼻拭子中从第7～22d均能检测到病毒的存在,表明患羊痘疹痂皮和鼻分泌物是山羊痘蔓延扩散的主要传染源。应用FQ-PCR方法在皮肤痘疹病变出现之前3～5d即可检测病毒的增殖动态,为山羊痘的早期诊断提供了实用的技术。
对山羊痘现场自然病例和人工复制病例进行病理形态学观察,患病羊群的大体病变以皮肤、呼吸道和消化道黏膜出现痘疹为特征;病变部位上皮细胞增生、变性,同时出现巨噬细胞、淋巴细胞和嗜中性白细胞等炎性细胞浸润;受害细胞胞浆内观察到线粒体肿胀、内质网扩张、高尔基复合体扩张甚至破裂。在感染细胞的胞浆中观察到大量成熟和未成熟的痘病毒颗粒,包括形态较大,被膜完整或不完整,呈C形或花瓣状的初期病毒颗粒;在中央或偏中央部位开始形成致密类核体的中期病毒颗粒;和形态较小,有囊膜包裹,中央可见两面凹陷呈哑铃形核酸芯髓的成熟期痘病毒颗粒。
将GPV-QL、GPV-LD、GPV-Y和GPV-B毒株的P32基因克隆至pMD18-T载体,重组质粒测序和序列分析显示,GPV贵州分离株之间核苷酸同源性高达99.9%,与国内其它GPV分离株的核苷酸同源性达到99.7%～100%,与国外GPV分离株核苷酸同源性达到99.5%～99.6%。系统发生树分析显示羊痘病毒属中SPV与LSDV之间亲缘关系较近,聚为一类,而它们与GPV亲缘关系较远,后者聚为一类,不同毒株之间表现出明显的种间差异和地域关系。P32蛋白的跨膜结构、亲水性、抗原指数、柔韧性、表面可及性和二级结构分析结果表明,GPV P32蛋白基因在肽段227-251区域存在一个潜在的优势抗原表位。
以pMD18-T-P32/LD质粒为模板,扩增P32基因不同长度的片段并克隆至表达载体,构建原核重组表达载体和真核重组表达载体,转化至相应的宿主菌中进行诱导表达。结果表明,P32基因N端1/3片段和中间1/3片段未能通过原核表达载体pET-28a、毕赤酵母真核表达载体pPICZaA在各自宿主菌BL21(DE3)、X-33中获得表达;而P32全基因在两种表达系统中均获得了良好的表达,SDS-PAGE和Western-Blotting分析显示表达蛋白分子量分别为35.5KD和64KD。表达蛋白通过Ni柱亲和层析和Sephadex G-100层析获得纯化,该纯化蛋白在琼脂扩散试验中与山羊痘阳性血清出现特异性的沉淀线,而与阴性血清不发生反应;采用纯化蛋白接种家兔制备免疫血清,该免疫血清使山羊痘病毒感染力减少50%的中和指数达到1:123.07。采用纯化的P32蛋白建立了检测山羊痘血清抗体的间接ELISA方法。
应用H.E染色法、凋亡试剂盒检测法、流式细胞仪检测法和DNA Ladder检测法证实GPV能够诱导BHK-21细胞发生凋亡,结果显示,GPV能引起BHK-21细胞凋亡。
Goat pox, which is listed in Group A diseases of the OIE and in Group one diseases of China, is a highly contagious viral disease of goats, characterized by fever, ocular and nasal discharges. Pox lesions appear on the skin, the respiratory and gastro-intestinal mucosae. The disease is caused mainly by goat pox virus (GPV) which is one of pox viruses, classified in the genus Capripoxvirus of the family Poxviridae. Since October 2002, the disease firstly outbroke in Guizhou province and spreaded quickly. The disease inflicts the production of goat cultivation in Guizhou province. We have isolated and verified the pathogen of the doubtful goat pox disease in Guizhou province. Then we studied the major structural protein gene P32 of GPV and the pathomorphology of clinical goat pox disease cases and artificial infection cases were observed. The mainly study content are followed.
The Vero-E6, BHK-21 cells were infected by GPV-LD and GPV-QL strains , then they showed some cytopathic effects after 3~7 days, such as getting to round, gathering to clustering. The fluorescent antibody test showed some specific flavovirens fluorescence in the cytoplasm. There were lots of mature and immature particles in the cytoplasm of infected cells under the transmission electron microscope(TEM). The genus special fragments P32 gene(969bp) and ITR gene(289) were amplified from the DNA samples which were extracted from the infected cell cultures by PCR, the results of sequencing showed the fragments were the special fragments in the GPV genome. The 2000TCID_(50) GPV-LD strain infected cell cultures were used to infect the 3 months old healthy goats through subcutaneous inoculation. The infected goats showed the same clinical symptom and pathological changes as the clinical cases of goat pox and the goat pox viruses were retrieved from the infected goats.
Two specific primers were designed according to the P32 gene and ITR gene sequences of goat poxvirus, the sensitivity and specificity were compared with each other. The results showed the primers have the specificity of Capripoxvirus and do not have consensual reaction with the Orf virus of parapoxvirus, Fowl pox virus of Avipoxvirus and the skin samples of healthy goats. The homologies of nucleotide were above 99.5% and 100% between P32 gene or ITR gene sequences of goat pox viruses. The minimum DNA detection amount of P32 gene primers and ITR gene primers were 19.06ng and 24.40pg. On the whole, the ITR primers are more fit used to diagnose the clinical goat pox disease, and the P32 primers are more fit used to research the virus membrane protein.
According to the goat pox virus complete gene sequence, a size of 64 bp gene fragment which locates in gp064 region of goat pox virus (GPV) genome was selected and a pair of primers and a TaqMan-MGB probe against the gene fragment were designed with Primer Express 2.0 software. Then, the fluorescence quantitative PCR (FQ-PCR) assay was developed for quantitative detection of goat pox cases. The FQ-PCR can detect the goat pox virus from the nose swabs, blood samples, skin samples, lung samples, stomach samples, et al., which were selected from GPV infected goats, while the common PCR can detect the GPV only from the skin samples and mucous membrane samples with exanthema variolosum. The minimum DNA detection amount of the FQ-PCR is 0.1 TCID_(50) according to the standard curve of this test. And the FQ-PCR can detect the exist of GPV before 3～5 days of the skin exanthema variolosum appearance. So the FQ-PCR is a useful technology for the technology of goat pox.
The pathomorphology of clinical goat pox cases and artificial infection cases in Guizhou province were observed. The major pathological changes were pox lesions on the skin, respiratory tract and gastro-intestinal tract. And there were hyperplasia and degeneration in epithelial cell and inflammatory cell in diseased regions, such as macrophages, leukomonocyte, leucocyte neutrophils, et al. The cytopathic effects such as cytochondriome tumentia, endocytoplasmic reticulum expansion and Golgi's complex expansion were also observed under the TEM. A lots of mature and immature particles in the cytoplasm of affected cells were observed under TEM, including initial stage particles, intermediate stage particles and mature particles. The phenomena that GPV could damage the vascular endothelial cells and medullated nerve fibers were finded under TEM. The test results remain that mature particles got the peplos through the cell organ membrane perhaps was one of the way for particles getting the peplos.
The P32 genes of GPV-QL, GPV-LD, GPV-Y and GPV-B were inserted into the pMD18-T vector. The sequence analysis of recombinant plasmids showed that the P32 gene of GPV strains isolated from Guizhou shared 99.9% nucleotides with each other, 99.5%～100% with the GPV strains of Chinese and 99.5%-99.6% with the GPV strains of foreign country. Amino acids homology is similar to the nucleotide homology. The Phylogenetic analysis showed the relationship of Guizhou strains were nearer with Chinese other strains than that of foreign strains. The research indicated the P32 gene of capripoxvirus is very conservative and the heredity of capripoxvirus has some relation with the geographic setting. The bioinformatics analysis showed that P32 protein was a kind of membrane protein which had a larvaceous dominant epitope in the 227-251 segment of the amino acid sequence.
The different P32 gene fragments were amplified by PCR method from pMD18-T-P32/LD recombinant plasmids. The different P32 gene fragments were cloned into prokaryotic expression vector and eukaryotic expression vector. The proteins were induced to express in their host bacterium and their antigenicity were researched. Then the ELISA for detecting the serum antibody of goat pox were established against the pure P32 protein. The results showed the P32/N_(1/3) and P32/M_(1/3) gene fragments did not express in both eukaryotic expression system and prokaryotic expression system, while the complete P32 gene expressed well in both eukaryotic expression system and prokaryotic expression system. The results of SDS-PAGE and Western-Blotting showed a specific protein band about 35.5KD in eukaryotic expression products and a specific protein band about 64KD in prokaryotic expression products were detected. The expression protein can be purified by Ni~+ affinity chromatograph and G-100 chromotography techniques. The agar diffusion reaction and animal experiment identified that the pure P32 protein had good antigenicity. The clinical experiment verified the indirect ELISA assay which made use of the pure P32 protein was useful for detecting the goat pox serum antibody.
For detecting the BHK-21 cell apoptosis caused by goat pox virus, the H.E staining method, apoptosis detection kit, flow cytometry and DNA Ladder test were used to detect the BHK-21 cell cultures which infected by the goat pox virus. The results showed that the goat pox virus could cause the BHK-21 cell apoptosis. The research consequences provide some evidences to explain some pathological changes of goat pox.
将贵州省山羊痘病毒分离株GPV-LD和GPV-QL接种Vero-E6、BHK-21细胞,3～7d感染细胞显现圆缩、聚集成簇等细胞病变效应;以GPV荧光抗体对感染细胞飞片染色,在细胞浆中检测到特异性的黄绿色荧光;取感染细胞超薄切片进行电子显微镜观察,细胞浆中发现大量成熟和未成熟的痘病毒颗粒;将感染细胞培养物提取总DNA样本,采用针对P32结构蛋白基因和ITR非编码区基因的两对引物进行PCR鉴定,分别扩增出969bp和289bp的DNA片段,对目的DNA片段的测序结果证实感染细胞培养物中存在GPV特异性的病原核酸;采用2000TCID_(50)的病毒感染细胞培养物通过皮下接种3月龄健康山羊,在14～45d成功复制出与山羊痘自然病例相类似的临床症状和剖检病变,并从感染组织材料中回收到接种的病毒。
对GPV结构蛋白P32基因和非编码区ITR基因的两对引物进行比较,以期开展临床山羊痘病例的定性PCR检测。研究结果表明两对引物具有羊痘病毒属特异性,不与副痘病毒属羊传染性脓疱病毒、禽痘病毒属鸡痘病毒、健康山羊皮肤样本发生交叉反应。贵州分离毒P32基因和ITR基因与国内外参考毒株的核苷酸同源性分别达99.5%～100%和100%;PCR最小检出量分别为19.06ng和24.40ng。为此,ITR基因引物更适合于临床山羊痘病例的诊断,而P32基因则有利于病毒囊膜表面蛋白的深入研究。
根据GPV参考毒株的全基因序列,针对gp064基因分别设计与合成了TaqMan-MGB探针和引物,应用实时荧光PCR方法开展对山羊痘病例的定量检测。一般PCR方法只能检测患羊出现痘疹病变的皮肤和黏膜材料,而FQ-PCR方法则能够从患羊鼻腔拭子、血液样本、皮肤、肺、胃、淋巴结等组织材料中检出GPV病原核酸。按照本试验构建的标准曲线,TaqMan-MGB-FQ-PCR方法的检测极限为0.1TCID_(50)的病毒量。检测结果表明在人工复制山羊痘病例中,不同组织的含毒量呈现皮肤＞瘤胃＞肺＞淋巴结的趋势;接种山羊从第9～15d形成病毒血症,持续时间较短;鼻拭子中从第7～22d均能检测到病毒的存在,表明患羊痘疹痂皮和鼻分泌物是山羊痘蔓延扩散的主要传染源。应用FQ-PCR方法在皮肤痘疹病变出现之前3～5d即可检测病毒的增殖动态,为山羊痘的早期诊断提供了实用的技术。
对山羊痘现场自然病例和人工复制病例进行病理形态学观察,患病羊群的大体病变以皮肤、呼吸道和消化道黏膜出现痘疹为特征;病变部位上皮细胞增生、变性,同时出现巨噬细胞、淋巴细胞和嗜中性白细胞等炎性细胞浸润;受害细胞胞浆内观察到线粒体肿胀、内质网扩张、高尔基复合体扩张甚至破裂。在感染细胞的胞浆中观察到大量成熟和未成熟的痘病毒颗粒,包括形态较大,被膜完整或不完整,呈C形或花瓣状的初期病毒颗粒;在中央或偏中央部位开始形成致密类核体的中期病毒颗粒;和形态较小,有囊膜包裹,中央可见两面凹陷呈哑铃形核酸芯髓的成熟期痘病毒颗粒。
将GPV-QL、GPV-LD、GPV-Y和GPV-B毒株的P32基因克隆至pMD18-T载体,重组质粒测序和序列分析显示,GPV贵州分离株之间核苷酸同源性高达99.9%,与国内其它GPV分离株的核苷酸同源性达到99.7%～100%,与国外GPV分离株核苷酸同源性达到99.5%～99.6%。系统发生树分析显示羊痘病毒属中SPV与LSDV之间亲缘关系较近,聚为一类,而它们与GPV亲缘关系较远,后者聚为一类,不同毒株之间表现出明显的种间差异和地域关系。P32蛋白的跨膜结构、亲水性、抗原指数、柔韧性、表面可及性和二级结构分析结果表明,GPV P32蛋白基因在肽段227-251区域存在一个潜在的优势抗原表位。
以pMD18-T-P32/LD质粒为模板,扩增P32基因不同长度的片段并克隆至表达载体,构建原核重组表达载体和真核重组表达载体,转化至相应的宿主菌中进行诱导表达。结果表明,P32基因N端1/3片段和中间1/3片段未能通过原核表达载体pET-28a、毕赤酵母真核表达载体pPICZaA在各自宿主菌BL21(DE3)、X-33中获得表达;而P32全基因在两种表达系统中均获得了良好的表达,SDS-PAGE和Western-Blotting分析显示表达蛋白分子量分别为35.5KD和64KD。表达蛋白通过Ni柱亲和层析和Sephadex G-100层析获得纯化,该纯化蛋白在琼脂扩散试验中与山羊痘阳性血清出现特异性的沉淀线,而与阴性血清不发生反应;采用纯化蛋白接种家兔制备免疫血清,该免疫血清使山羊痘病毒感染力减少50%的中和指数达到1:123.07。采用纯化的P32蛋白建立了检测山羊痘血清抗体的间接ELISA方法。
应用H.E染色法、凋亡试剂盒检测法、流式细胞仪检测法和DNA Ladder检测法证实GPV能够诱导BHK-21细胞发生凋亡,结果显示,GPV能引起BHK-21细胞凋亡。
Goat pox, which is listed in Group A diseases of the OIE and in Group one diseases of China, is a highly contagious viral disease of goats, characterized by fever, ocular and nasal discharges. Pox lesions appear on the skin, the respiratory and gastro-intestinal mucosae. The disease is caused mainly by goat pox virus (GPV) which is one of pox viruses, classified in the genus Capripoxvirus of the family Poxviridae. Since October 2002, the disease firstly outbroke in Guizhou province and spreaded quickly. The disease inflicts the production of goat cultivation in Guizhou province. We have isolated and verified the pathogen of the doubtful goat pox disease in Guizhou province. Then we studied the major structural protein gene P32 of GPV and the pathomorphology of clinical goat pox disease cases and artificial infection cases were observed. The mainly study content are followed.
The Vero-E6, BHK-21 cells were infected by GPV-LD and GPV-QL strains , then they showed some cytopathic effects after 3~7 days, such as getting to round, gathering to clustering. The fluorescent antibody test showed some specific flavovirens fluorescence in the cytoplasm. There were lots of mature and immature particles in the cytoplasm of infected cells under the transmission electron microscope(TEM). The genus special fragments P32 gene(969bp) and ITR gene(289) were amplified from the DNA samples which were extracted from the infected cell cultures by PCR, the results of sequencing showed the fragments were the special fragments in the GPV genome. The 2000TCID_(50) GPV-LD strain infected cell cultures were used to infect the 3 months old healthy goats through subcutaneous inoculation. The infected goats showed the same clinical symptom and pathological changes as the clinical cases of goat pox and the goat pox viruses were retrieved from the infected goats.
Two specific primers were designed according to the P32 gene and ITR gene sequences of goat poxvirus, the sensitivity and specificity were compared with each other. The results showed the primers have the specificity of Capripoxvirus and do not have consensual reaction with the Orf virus of parapoxvirus, Fowl pox virus of Avipoxvirus and the skin samples of healthy goats. The homologies of nucleotide were above 99.5% and 100% between P32 gene or ITR gene sequences of goat pox viruses. The minimum DNA detection amount of P32 gene primers and ITR gene primers were 19.06ng and 24.40pg. On the whole, the ITR primers are more fit used to diagnose the clinical goat pox disease, and the P32 primers are more fit used to research the virus membrane protein.
According to the goat pox virus complete gene sequence, a size of 64 bp gene fragment which locates in gp064 region of goat pox virus (GPV) genome was selected and a pair of primers and a TaqMan-MGB probe against the gene fragment were designed with Primer Express 2.0 software. Then, the fluorescence quantitative PCR (FQ-PCR) assay was developed for quantitative detection of goat pox cases. The FQ-PCR can detect the goat pox virus from the nose swabs, blood samples, skin samples, lung samples, stomach samples, et al., which were selected from GPV infected goats, while the common PCR can detect the GPV only from the skin samples and mucous membrane samples with exanthema variolosum. The minimum DNA detection amount of the FQ-PCR is 0.1 TCID_(50) according to the standard curve of this test. And the FQ-PCR can detect the exist of GPV before 3～5 days of the skin exanthema variolosum appearance. So the FQ-PCR is a useful technology for the technology of goat pox.
The pathomorphology of clinical goat pox cases and artificial infection cases in Guizhou province were observed. The major pathological changes were pox lesions on the skin, respiratory tract and gastro-intestinal tract. And there were hyperplasia and degeneration in epithelial cell and inflammatory cell in diseased regions, such as macrophages, leukomonocyte, leucocyte neutrophils, et al. The cytopathic effects such as cytochondriome tumentia, endocytoplasmic reticulum expansion and Golgi's complex expansion were also observed under the TEM. A lots of mature and immature particles in the cytoplasm of affected cells were observed under TEM, including initial stage particles, intermediate stage particles and mature particles. The phenomena that GPV could damage the vascular endothelial cells and medullated nerve fibers were finded under TEM. The test results remain that mature particles got the peplos through the cell organ membrane perhaps was one of the way for particles getting the peplos.
The P32 genes of GPV-QL, GPV-LD, GPV-Y and GPV-B were inserted into the pMD18-T vector. The sequence analysis of recombinant plasmids showed that the P32 gene of GPV strains isolated from Guizhou shared 99.9% nucleotides with each other, 99.5%～100% with the GPV strains of Chinese and 99.5%-99.6% with the GPV strains of foreign country. Amino acids homology is similar to the nucleotide homology. The Phylogenetic analysis showed the relationship of Guizhou strains were nearer with Chinese other strains than that of foreign strains. The research indicated the P32 gene of capripoxvirus is very conservative and the heredity of capripoxvirus has some relation with the geographic setting. The bioinformatics analysis showed that P32 protein was a kind of membrane protein which had a larvaceous dominant epitope in the 227-251 segment of the amino acid sequence.
The different P32 gene fragments were amplified by PCR method from pMD18-T-P32/LD recombinant plasmids. The different P32 gene fragments were cloned into prokaryotic expression vector and eukaryotic expression vector. The proteins were induced to express in their host bacterium and their antigenicity were researched. Then the ELISA for detecting the serum antibody of goat pox were established against the pure P32 protein. The results showed the P32/N_(1/3) and P32/M_(1/3) gene fragments did not express in both eukaryotic expression system and prokaryotic expression system, while the complete P32 gene expressed well in both eukaryotic expression system and prokaryotic expression system. The results of SDS-PAGE and Western-Blotting showed a specific protein band about 35.5KD in eukaryotic expression products and a specific protein band about 64KD in prokaryotic expression products were detected. The expression protein can be purified by Ni~+ affinity chromatograph and G-100 chromotography techniques. The agar diffusion reaction and animal experiment identified that the pure P32 protein had good antigenicity. The clinical experiment verified the indirect ELISA assay which made use of the pure P32 protein was useful for detecting the goat pox serum antibody.
For detecting the BHK-21 cell apoptosis caused by goat pox virus, the H.E staining method, apoptosis detection kit, flow cytometry and DNA Ladder test were used to detect the BHK-21 cell cultures which infected by the goat pox virus. The results showed that the goat pox virus could cause the BHK-21 cell apoptosis. The research consequences provide some evidences to explain some pathological changes of goat pox.
引文
1.才旦.绵羊痘的诊断与防治[J].中国兽医杂志,2004,3:53-54.
2.程振涛,徐春志,岳筠,李永明,许乐仁,周碧君,文明.山羊痘病毒不同基因片段PCR诊断方法的研究[J].黑龙江畜牧兽医,2008,33(3):63-64.
3.程振涛,岳筠,李永明,许乐仁,王开功,周碧君,陈军义,李俊,江楠.山羊痘病毒TaqMan-MGB荧光定量PCR检测方法的建立与应用.生物工程学报,2009,25(3):464-472.
4.邓继军,周霞.山羊痘的诊治[J].吉林畜牧兽医,2004,(11):21.
5.丁打海.中国进出口动物检疫规范[M].中国农业出版社,1997,502-507.
6.丁选亚,乔传玲,陈艳,杨焕良,辛晓光,韩庆功,陈化兰.H3N2亚型猪流感病毒重组HA1蛋白间接ELISA诊断方法的建立[J].畜牧兽医学报,2008,39(9):1230-1234.
7.郭爱珍.山羊痘暴发流行的诊断[J].中国兽医杂志.2001:9.
8.郭建刚,郑敏,刘棋,李华明,邓朝阳,邹联斌,姚火春.山羊痘病毒P32基因在杆状病毒中的表达[J].中国兽医科技,2005,35(12):950-953.
9.郭巍,陈杰,黄保续,李晓成,吴发兴,张燕霞,李一经.应用PCR检测山羊痘病毒[J].中国兽医科技,2003,33(12):50-52.
10.郭巍,相文华,李一经.羊痘病毒P32蛋白的表达[J].中国兽医杂志,2005,41(3):8-10.
11.郭艳,付朝阳,宋素泉,高宏雷,王笑梅,王英,张厚双,王晓艳,胡建民.J亚群禽白血病ELISA抗体检测方法的建立.中国预防兽医学报,2005,27(4):304-307.
12.韩剑锋,宁宜宝,宋立.实时荧光PCR技术快速鉴别检测H5、H9、H7亚型禽流感灭活疫苗的研究[J]_生物工程学报,2007,23(5):953-957.
13.何光志,周碧君,虞天德,许乐仁.贵州山羊痘的病原学研究与血清学检测.山地农业生物学报,2004,23(4):300-304.
14.洪燕,谢忠文,施恒豫.人感染山羊痘五例[J].中华传染病杂志,2005,23(2):143.
15.金洪峰,韩梅.绵羊痘对养羊业的危害及防治基本对策[J].黑龙江畜牧兽医,2002,11:30-31.
16.康文玉,徐自忠,花群义,杨云庆,周晓黎,董俊,尹尚莲,高洪.羊痘病毒P32蛋白编码基因的克隆及表达[J].中国兽医科学,2006,36(6):454-459.
17.康文玉,徐自忠,高洪,花群义,周晓黎,杨云庆,董俊.羊痘病毒.中国畜牧兽医.2004,12:33-36.
18.李普森编著 食用动物疾病病理学[M]吉林科学技术出版社1989.5
19.李振刚主编,分子遗传学.北京,科学出版社,2000.
20.廖振续.山羊痘的暴发与防制[J].中国兽医杂志,1997,3:31-32.
21.林敏,倪凤娥.绵羊痘的诊治[J].河北畜牧兽医,2002,(10):33.
22.刘国英,乌日娜,李荣,等.牛睾丸原代细胞生产羊痘疫苗的试验[J].中国兽药杂志,2002,36(10):41.
23.刘棋,黄夏,郭建刚,陈义祥,郑敏,邓朝阳,李华明,邹联斌.山羊痘病毒的分离鉴定及生物学特性的研究[J].中国预防兽医学报,2006,(5):494-498.
24.龙沛然,周卫国,覃敏.山羊痘病毒的电子显微镜观察[J].中国兽医杂志,2004,40(8):23-24.
25.卢圣栋主编.现代分子生物学实验技术.北京,中国协和医科大学出版社,1999.
26.陆承平主编.兽医微生物学.北京,中国农业出版社,2001.
27.罗辽复,李晓琴.tRNA丰度是影响蛋白质二级结构形成的一个因素[J].内蒙古大学学报,2003,34(5):519-529.
28.马世东.非洲猪瘟的间接ELISA诊断试剂盒的研究[D].新疆农业大学硕士论文.
29.娜仁.绵羊痘的防治[J].中国动物保健,2002,5:49.
30.彭毅,步威,康良仪.甲醇酵母表达系统.生物技术通报,2000.(1):38-41.
31.瞿礼嘉等著.现代生物技术导论(第一版).高等教育出版社,北京,1998.
32.任增亮,堵国成,陈坚.大肠杆菌高效表达重组蛋白策略[J].中国生物工程杂志,2007,27(9):103-109.
33.萨姆布鲁克J,拉赛尔D W.分子克隆实验指南.[M].科学出版社.2002.
34.时成波,吕安国,吴文芳,杨立泉,冯家勋,柏学亮.改造稀有密码子提高SEA蛋白表达量[J].生物工程学报,2002,18(4):477-480.
35.世界动物卫生组织.哺乳动物、禽、蜜蜂A和B类疾病诊断试验和疫苗标准手册[M].农业部畜牧兽医局.中国农业科学技术出版社,2002:156-166.
36.孙桂玲,李志刚,宋明,林森.羊痘病的防治[J].吉林畜牧兽医,2004,6:42-43.
37.索效军,张年,李晓峰,付忠燕,陈明新.羊痘的诊断与防治[J].湖北畜牧兽医,2004,6:29-30.
38.王殿军.绵羊痘的防治[J].中兽医医药杂志.1995,1:47.
39.王开功,虞天德,何光志,周碧君,许乐仁,孙道忠.应用反向间接血凝试验检测山羊痘病毒抗原.山地农业生物学报,2005,24(1):29-32.
40.王开功,虞天德,张大权,田志敬,阴正兴,许乐仁,周碧君.贵州省首次暴发山羊痘的诊断研究.动物医学进展,2003,24(4):116-118.
41.王效田,兰朋云,夏明龙,等.山羊痘的诊断与防制[J].中国兽医杂志,2001,37(3):28-29.
42.文明,程振涛,岳筠,李永明,周碧君.山羊痘病毒P32基因序列分析及其B细胞表位预测.生 物技术,2007,17(5):12-14.
44.吴乃虎主编.基因工程原理(第2版,上册).北京,科学出版社,2001
45.许乐仁,周碧君,阴正兴.山羊痘病变的超微病理学研究[J].畜牧兽医学报,2005,36(5):492-497.
46.许乐仁,周碧君.山羊痘的研究概况[J].内蒙古畜牧科学,2003,4:13-15.
47.阴正兴,许乐仁,周碧君,虞天德,乐正中.山羊痘的病理形态学观察[J].中国预防兽医学报,2005,27(5):402-407.
48.殷震,刘景华主编.动物病毒学(第二版)[M].北京:科学出版社,1997,960-963.
49.俞乃胜.山羊疾病学[M].云南科技出版社,2000.
50.张端阳,周永安,王俊民.羊痘一家4例报告[J].中国皮肤性病学杂志,2003,3:196-197.
51.张前群,马承俊.山羊痘暴发流行的防制与思考[J].畜禽业,2004,9:20.
52.张小青,丁明孝.痘苗病毒诱导HeLa细胞的凋亡[J].中国病毒学,1998,13(4):364-368.
53.张振岚,夏明龙.南京部分地区暴发流行山羊痘的诊断与防制[J]畜牧与兽医2000,32(4)23-24.
54.周碧君,虞天德,阴正兴,许乐仁.山羊痘病毒粒子的电子显微镜观察[J].中国预防兽医学报,28(3):316-319.
55.周锦萍.山羊痘病毒的分离与鉴定[J].上海畜牧兽医通讯,2002,(3):44.
56.周则迅,袁汉英,何炜.乙肝病毒表面抗原SA-28融合基因在酵母中的组成型表达[J].复旦学报(自然科学版),2000,39(3):264-268.
57.Adlakha S C,Bansal M P and Malik B S.Studies on sheep and goat-poxviruses I.Adaptation of the viruses in laboratory animals and tissue culture[J].Indian J Anim Sci,1971,41:171-175.
58.Allerson CR,Martinez A,Yikilmaz E,Rouault TA.A high-capacity RNA affinity column for the purification of human IRP1 and IRP2 overexpressed in Pichia pastoris[J].Rna-A Publication of the Rna Society,2003,9(3):364-374.
59.Annadana S,Schipper B,Beekwilder J,Outchkourov N,Udayakumar M,Jongsma MA.Cloning.functional expression in Pichia pastoris,and purification of potato cystatin and multicystatin[J].Journal of Bioscience & Bioengineering,2003,95(2):118-123.
60.Arnau J,Lauritzen C,Petersen GE,Pedersen J.Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins[J].Protein Expr Purif,,2006,48(1):1-13.
61.Baixeras E.,Cebrian,A.Vaccinia virus-induced apoptosis in immature B lymphocytes:role of cellular Bcl-2[J].Virus Research,1998,58:107-113
62. Bei JL, Wang JW, Wang XZ, Long QX, Yang L, Deng YY. High expression of LIP1 in Pichia pastoris[J]. Acta Biochimica & Biophysica Sinica, 2003, 35 (4): 366-370.
63. Bowers LM, Lapoint K, Ant hony L, Pluciennik A, Filutowicz M.Bacterial expression system with tightly regulated gene expression and plasmid copy number [J] .Gene, 2004, 340 (1):11-18.
64. Cam V M, Kitching R P, Hammond J M., Chand P. Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus[J]. J. Virol. Methods, 1994,49:285-294.
65. Cam V M. An antigen trapping ELISA for the detection of capripoxvirus in tissue culture supernatant and biopsy samples[J]. J. Virol. Methods, 1995, 51: 95-102.
66. Cam V M. Control of capripoxvirus infections[J]. Vaccine, 1993,11:1275-1279.
67. Cam V. M., Kitching R. P., Hammond J. M., et al. Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus[J]. J. Virol. Methods, 1994,49:285-294.
68. Chadd H E , Chamow S M. Therapeutic antibody expression technology [J]. Curr Opin Biotechnol, 2001,12: 188-194.
69. Chand P, Kitching R P & Black D N. Western blot analysis of virus-specific antibody responses to capripoxvirus and contagious pustular dermatitis infections in sheep[J]. Epidemiol. Infect,1994,113:377-385.
70. Chen H , Xu Z, Cen P. High-level expression of human beta-defensin-2 gene with rare codons in E.coli cell-free system [J].Protein Pept Lett, 2006, 13 (2) : 155-162.
71. Chou P. Y, Fasman G.D. Prediction of the secondary structure of protein conformation[M].New York: Plenum Press, 1990, 549-589.
72. Chumpolkulwong N , Aakamoto K, Hayashi A , Iraha F, Shinya N, Matsuda N, Kiga D,Urushibata A, Shirouzu M, Oki K, Kigawa T, Yokoyama S. Translation of 'rare' codons in a cell-free protein synthesis system from Escherichia coli [J]. J Struct Funct Genomics, 2006, 7(1): 31-36.
73. Cran V M. Control of capripoxvirus infection[J].Faccine,1993,11(3):1275-1279.
74. Cregg J M, Madden K R, Barringer K J, Thill GP, Stillman CA. Functional characterization of the two alcohol oxidase genes from the yeast pichia pastoris[J]. Mol Cell Biol, 1989, 9:1316-1323.
75. Cregg J M, Vedvick T S, Raschke W C. Resent advances in the expression of foreign genes in pichia pastoris[J]. Bio/Technology, 1993, 11:905-910.
76. David R, Higgins, James M. Pichia Protocols. New jersey, Humana Press Inc, 1998:193-200
77. Davies F G, Krauss H, Lund L J, Taylor M. The laboratory of lumpy skin disease[J]. Res Vet Sci, 1971,12:123-127.
78.Davies F G.& Atema C.The antibody response in sheep infected with a Kenyan sheep and goat pox virus[J].J Comp.Pathol.,1978,88:205-210.
79.Davies F G.& Mbugwa G.The alterations in pathogenicity and immunogenicity of a Kenya sheep and goat pox virus on serial passage in bovine fontal muscle cell cultures[J].J.Comp.Pathol.,1985,95:565-576.
80.Davies FG.Characteristics of a virus causing a pox disease in sheep and goats in Kenya with observations on the epidemilogy and control[J].Journal of Hygiene,Cambridge,1976,76:163-171.
81.Dong X,Tang B,Li J,Xu Q,Fang S,Hua Z.Expression and Purification of Intact and Functional Soybean(Glycine max) Seed Ferritin Complex in Escherichia coli[J].J Microbiol Biotechnol,2008,18(2):299-307.
82.Ellis S B,Brust P F,Koutz P J,Waters AF,Harpold MM,Gingeras TR.Isolation of alcohol oxidase and two other methanol requlatable genes from the yeast pichia pastoris[J].Mol Cell Biol,1985,5:1111-1121.
83.Emini E.A,Hughes J.V,Perlow D.S,Boger J.Induction of hepatitis a virus-neutralizing antibody by a virus-specific synthetic peptide[J].J Virol.,1985,55:836-839.
84.Esposito J.P and F.Fenner.Poxviruses.2001.2885-2921
85.Fidler A E,Lin J S,Chie W N,Ng Chie W,Western A,Stent V,McNatty KP.Production of biologically active tethered ovine FSH beta alpha by the methylotrophic yeast Pichia pastoris[J].J Mol Endocrinol,2003,30(2):213-225.
86.Garner M G,Sawarkar S D,Brett E K,Edwards JR,Kulkarni VB,Boyle DB,Singh SN.The extent and impact of sheep pox and goat pox in the state of Maharashtra,India[J].Trop Anim Health Prod,2000,32(4):205-23.
87.Garnier J,Osguthorpe D.J and Robson B.Analysis of the accuracy and implications of simple method for prediction the secondary structure of globular proteins[J].J..Mol.Biol.,1978,12:97-120.
88.Gen Wang,John W.Myxoma Virus M11L Prevents Apoptosis through Constitutive Interaction with Bake[J].Journal of Virology,2004,(7):7097-7111
89.Georgiou G,Segatori L.Preparative expression of secreted proteins in bacteria:status report and future prospects[J].Curr Opin Biotechnol,2005,16(5):538-545.
90.Gershon P.D.,Ansell D.M.and Black D.N.A comparison of the genome organization of capripoxvirus with that of the orthopoxviruses[J].J Virol.,1989,63(11):4703-4708.
91. Gershon P.D., and Black D.N. The nucleotide sequence around the capripoxvirus thymidine kinase gene reveals a gene shared specifically with leporipoxvirus[J]. J Gen Virol., 1989,70(3):525-533.
92. Gershon, P.D., Black.A. Capripoxvirus pseudogene whose only intact homologys are in other poxvirus genomes[J]. Virology, 1989, 172:350-354.
93. Hanquier J, Sorlet Y, Desplancq D, Baroche L, Ebtinger M, Lefevre JF, Pattus F, Hershberger CL, Vertes AA. A single mutation in the activation site of bovine trypsinogen enhances its accumulation in the fermentation broth of the yeast Pichia pastoris[J]. Applied & Environmental Microbiology, 2003,69 (2): 1108-1113.
94. Hay S., Kannourakis G. A time to kill: viral manipulation of the cell death program[J]. J Gen Virol, 2002, 83: 1547-1564.
95. Heine H. G., Stevens M. P., Foord AJ. A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog cf the vaccine virus H3L gene[J]. Journal of Immunological Methods, 1999, 227:187-196.
96. Heine H.G, Lin C.L., Chung C.S. Vaccine virus envelope H3L protein binds to cell surface heparan sulfate and is important for intracellular mature virion morphogenesis and virus infection in vivo[J].Virol.2000,74(4):3353-3365.
97. Hirokawa T, Boon-Chieng S. and Mitaku S. SOSUI: Classification and secondary structure prediction system for membrane proteins [J]. Bioinformatics, 1998,14(4): 378-379.
98. Hoffman SK, Susani M, Ferreira F, Jertschin P, Ahorn H, Steiner R, Kraft D, Scheiner O,Breiteneder H. High-level expression and purification of the major birch pollen allerge, BetV [J].Protein Expr Purif., 1997, 9 (1): 33-39.
99. Hopp T.P, Woods K. R. Prediction of protein antigenic determinants from amino acid sequence[J].Proc. Natl. Acad Sci USA,1981,78(6): 3824-3828.
100. Hosamani M, Nandi S, Mondal B, Singh RK, Rasool TJ, Bandyopadhyay SK. A Vero cell-attenuated Goatpox virus provides protection against virulent virus challenge[J]. Acta Virol.,2004,1(48):15-21.
101. Hosamni M, Mondal B, Tembhurne P A, Bandyopadhyay SK, Singh RK, Rasool TJ.Differentiation of Sheep Pox and Goat Poxviruses by Sequence Analysis and PCR-RFLP of P32 Gene[J].Virus Genes, 2004,29(1):73-80.
102. Inouye M. The discovery of mRNA interferases: implication in bacterial physiology and application to biotechnology [J] J Cell Physi-ol., 2006, 209 (3): 670-676.
103. Ireland D.C, Binepal Y.S. Improved detection of capripoxvirus in biopsy samples by PCR[J].Journal of Virological Methods, 1998, 74:1-7.
104.Ivanov A V,Korovina A N,Tunit skaya,Kostyuk DA,Rechinsky VO,Kukhanova MK,Kochetkov SN.Development of the system ensuring a high-level expression of hepatitis C virus non-structural NS5B and NS5A proteins[J].Protein Expr Purif,2006,48(1):14-23.
105.Jahic M,Gustavsson M,Jansen A K,Martinelle M,Enfors SO.Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes[J].J Biotechnol,2003,102(1):45-53.
106.Jameson B.A,Wolf H.The antigenic index:A novel algorithm for prediction antigenic determinants[J].Comput.Appl.Biosci.,1988,4:181-186.
107.Jin Su,Gen Wang.Myxoma Virus M11L Blocks Apoptosis through Inhibition of Conformational Activation of Bax at the Mitochondria[J].Journal of Virology,2006,80(3):1140-1151
108.Jinbiao L,Dong W,Guozhi W,Wang G.High-level expression and single-step purification of recombinant Bacillus anthracis protective antigen from Escherichia coli[J].Biotechnol Appl Biochem,2008,4(7):102-110.
109.Johnson K L.,Vaillant F.,Lawen A.Protein tyrosine kinase inhibitors prevent didemnin B-induced apoptosis in HL-60 cells[J].FEBS Lett.,1996,383:1-5.
110.Jonasson P.,Liljeqvist S.,Nygren P A.,Stahl S.Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli[J].Biotechnology and Applied Biochemistry,2002,35(2):91-105.
111.Joseph M.,Fernandez,James P.Gene Expression Systems[M].New-york,Academic Press,1999.
112.Joshi R K,Chandra R,Rao V D P.Cytopathogenicity of goat poxvirus[J].Indian Journal of Comparative Microbiology Immunology and Infectious Diseases,1994,15:35-36.
113.Joshi R K.Growth behavior of sambalpur strain of goat poxvirus on chorioallantoic membrane of developing chicken embryo[J].Indian Journal of Virology,1995,12:109-111.
114.Julius D,Brake A,Blair L,Kunisawa R,Thorner J.Isolation of putative structural gene for the lysine-arginine-cleaving endopeptidese required for processing of yeast prepro-a-factor[J].Cell,1985,37:1075.
115.Kang Y,Son M S.,Hoang TT.One step engineering of T7-expression strains for protein production:increasing the host-range of the T7-expression system[J].Protein Expr Purif.,2007,55(2):325-333.
116.Karplus P.A,Schultz G.Prediction of chain flexibility in proteins[M].Naturwissenschaften,1985,72:212-213.
117.Kawai R,Yoshida M,Tani T,Igarashi K,Ohira T,Nagasawa H,Samejima M.Production and characterization of recombinant Phanerochaete chrysosporium beta-glucosadase in the methylotrophic yaest Pichia pastoris[J].Bioscience Biotechnology & Biochemistry,2003,67(1): 1-71
118.Kitching R P.Progress towards sheep and goat pox vaccines[J].Vaccine,1983,10(1):4-9.
119.Kitching R P,Hammond J M,Black D N.Studies on the major common precipitating antigen of capripoxvirus[J].J Gen Virol,1986,67(Pt1):139-148.
120.Kitching R P,Taylor W P.Clinical and antigenic relationship between isolates of sheep and goat pox viruses[J].Tropical Animal Health and Production,1985,17(2):64-74121.Kitching R P.Sheep and goat poxviruses[J].In:Webster RG,Granoff A,Encylopedia of Virology.London:Academic Press,1994,1160-1165
122.Koutz P,Davis G R,Stillman C,Barringer K,Cregg J,Thill G.Structural comparison of the Pichia Pastoris alcohol oxidase genes[J].Yeast,1989,5:167-177.
123.Kuba H,Furukawa A,Okajima T,Furukawa K.Efficient bacterial production of functional antibody fragments using a phagemid vector[J].Protein Expr Purif,2008,58(2):292-300.
124.Kyte J,Doolittle R.F.A simple method for displaying the hydropathic character of a protein[J].J Mol.Biol.,1982,157:105-132.
125.Li Y H.,Bai J J.,Jian Q..Expression of common carp growth hormone in the yeast Pichia pastoris and growth stimulation of juvenile tilapia(Oreochromis niloticus)[J].Aquaculture,2003,216(1-4):329-341.
126.Liang R.,Liu J.,Ren Q.,Ren Q,Liang P,Lin Z,Xie X.A T7-expression system under temperature control could create temperature-sensitive phenotype of target gene in Escherichia coli[J].J Microbiol Methods,2007,68(3):497-506.
127.Mac W T.High-Yield Secretion of recombinant geltions by Pichia Pastris[J].Yeast,1999,15:1087-1096.
128.Madhusudan hosamani,Bimalendu mondal,Prabhakar a.tembhume.Differentiation of Sheep Pox and Goat Poxviruses by Sequence Analysis and PCR-RFLP of P32 Gene[J].Virus Genes,2004,29(1):73-80.
129.Manganal-Vougiouka O.,Markoulatos P.,Koptopoulos G.,Nomikou K,Bakandritsos N,Papadopoulos P.Sheep poxvirus identification from clinical specimens by PCR,cell culture,Immunofluorescence and agar gel immunoprecipitation assay[J].Molecular and Cellular Probes,2000,14:305-310.
130.Mangana-Vougiouka O.,Markoulatos P.,Koptopoulos G.,Koptopoulos G,Nomikou K,Bakandritsos N,Papadopoulos O..Sheep poxvirus identification by PCR in cell cultures[J].J Virol Methods,1999,77(1):75-79.
131.Markoulatos P.,Manganal-Vougiouka O.Detection of sheep poxviruse skin biopsy samples by a multiplex polymerase chain reaction[J].Journal of Virological Methods,2000,84(2):161-167.
132.Marc W T.High-yield secretion of recombinant gelation by pichia pastoris[J].yeast,1999,15:108-109.
133.Matthews R E F.Classification and nomenclature of viruses[M].Intervirology,1982,17:1-199.
134.Michael J O.Donbue.Over expression in Pichia Pastries and crystallization of an elicidor protein secreted by the phytopathogenic fungus[J].Protein Experessionand Purification,1996,8:254-261.
135.Nagpal A K,Vihan V S,Singh D.,Edwards JR,Kulkarni VB,Boyle DB,Singh SN.Epidemiological study of an outbreak of goat-pox and its effect on the performance of kids[J].Indian Journal of Animal Sciences,1990,60(5):544-546
136.Nomura S I,Inamori K I,Muta T,Yamazaki S,Sunakawa Y,Iwanaga S,Takeshige K.Purification and characterization of human soluble CD14 expressed in Pichia pastoris[J].Protein Expression & Purification,2003,28(2):310-320.
137.Nourizad N.,Ehn M.,Gharizadeh B.,Hober S,Nyren P.Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase(apyrase) from potato tubers(Solanum tuberosum)[J].Protein Expression & Purification,2003,27(2):229-237.
138.Nuc P.,Nuc K.Recombinant protein production in Escherichia coli[J].Postepy Biochem,2006,52(4):448-456.
139.Ou J,Yamada Y,Nagahisa K,Hirasawa T,Furusawa C,Yomo T,Shimizu H.Dynamic change in promoter activation during lysine biosynthesis in Escherichia coli cells[J].Mol Biosyst,2008,4(2):128-134.
140.Plowright W.,Ferris P.D.The growth and cytopathogenicity of sheep poxvirus in tissue culture[J].Br.J..Exp.Pathol.,1958,39:424-435.
141.Quyen D T.,Schmidt-Dannert C.,Schmid RD.High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichia pastoris and some properties of the recombinant lipase[J].Protein Expression & Purification,2003,28(1):102-110.
142.Rafyi A,Ramyar H.Goat pox in Iran,serial passage in goats and the developing eggs and relationship with sheep pox[J].J Comp Pathol,1959,69(2):141-147.
143.Ran T V,Bandyopadhyay S K.A comprehensive review of goat pox and sheep pox and their diagnosis[J].Anim Health Res Rev,2000,1(2):127-136.
144.Rao T V,Malik P,Nandi S,Negi BS.Evaluation of immunocapture ELISA for diagnosis of goat pox[J].Acta Virol,1997,41(6):345-348.
145.Rao T V,Negi B S.Evaluation of different serological tests for the diagnosis of goat pox using soluble antigen[J].Trop Anim Health Prod.,1997,29(4):235-239.
146.Rao T.V.S.and Bandyopadhyay S.K.A comprehensive review of goat pox and sheep pox and their diagnosis[J].Animal Health Research Reviews,2000,1(2);127-136
147.Romanos M A,Scorer C A,Clare J J.Foreign gene expression in yeast:a review[J].Yeast,1992,8(6):423-488.
148.Romanos M A.Advances in the use of Pichia pastoris for high-level gene expression[J].Current Opinion in Biotechnology,1995,6:527-533.
149.Roulston A,Marcellus R C,Branton P E.Viruses and apoptosis[J].Annual Rev Microbiol,1999,53:577-628.
150.Sato T,Terabe M,Watanabe H.Codon and base biases after the initiation codon of the open reading frames in the Escherichia coli genome and their influence on the translation efficiency[J].J Biochem(Tokyo),2001,129(6):851-860.
151.Scorer C A,Clare J J,Mccombie W R.,Romanos MA,Sreekrishna K.Rapid selection using G418 of high copy number tramsformants of pichia pastoris for high-level foreign gene expression[J].Bio/Technology,1994,12:181-174.
152.Sedhukhan T,Nag NCl.Application of dot-ELISA in the diagnosis of goat pox outbreak[J].Indian Veterinary Journal,1998,75:841-842.
153.Shchelkunov S N.The orthopoxviral genome[M].Mol Biol.,1996,30:1-16.
154.Shi X Z,Karkut T,Chamankhah M,Alting-Mees M,Hemmingsen SM,Hegedus D.Optimal conditions for the expression of a single-chain antibody(scFv) gene in Pichia pasitoris[J].Protein Expression & Purification,2003,28(2) 321-330.
155.Siegei R S.Methylotroyphic yeast Pichia pastoris produced in high-cell-density fementation with high cell yields as vehide for recombinant protein production[J].Biotechnology and Bioegineering,1989,34:403-404.
156.Siegel R S,Buckholz R G,Thill GP.Production of epidermal growth factor in methylogrophic yeast cells[M].European Patent Application,1990.
157.Singh B,Rai A.Virion polypeptides of sheep poxvirus[J].Indian Vet Med J,1991,15(4):239-244.
158.Tantawi H.H,Shony M.O,Hassan F.K.Isolation and identification of the Sersenk strain of goat pox virus in Iraq[J].Trop Anim Health Prod,1979,11(4):208-210.
159.Terpe K.Overview of bacterial expression systems for heterologous protein production:from molecular and biochemical fundamentals to commercial systems[J].Appl Microbiol Biotechnol,2006,72(2):211-222.
160.Trinh L B.,Phue J N.,Shiloach J.Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris[J].Biotechnology & Bioengineering,2003,82 (4):438-444.
161.Tschopp J F,Sverlow G,Kosson R.High-level secretion of glycosylated invertase in the methylotrophic yeast,Pichia pastoris[J].Bio Technology,1987,5:1305-1308.
162.Tulman E R,Afonso C.L,Lu Z,Zsak L,Sur JH,Sandybaev NT,Kerembekova UZ,Zaitsev VL,Kutish GF,Rock DL.The genomes of sheeppox and goatpox viruses[J].J.Virol.,2002,76(12):6054-6061.
163.Tulman E R,Afonso C L.,Kutish G F,Zsak L,Sur JH,Sandybaev NT,Kerembekova UZ,Zaitsev VL,Kutish GF,Rock DL.Genome of lumpy skin disease virus[J].J.Virol,2001,75:7122-7130.
164.Ved Prakash,Chandra R,Rao V D P.Cultivation of goat pox virus in establish cell line[J].Indian J Virol,1994,10:60-63.
165.Wang W,Sun Y H,Wang Y P,Zhu ZY.Expression of grass carp growth hormone in the yeast Pichia pastoris[J].Acta Genetica Sinica,2003,30(4):301-306.
166.Waterham H R,Digan M E,Koutz P J,Lair SV,Cregg JM.Isolation of the Pichia pastoris glyceraldehyde-3-phosphate deydyogenase gene and regulation and use of its promoter[J].Gene,1997,186:37-44.
167.Wegner G.Emerging applications of methytrophic yeasts FEMS[J].Microbiol Rev,1990,87:279-284.
168.Wei HY,Jiang L F,Xue YH,Fang DY,Guo HY.Secreted expression of dengue virus type2full-length envelope glycoprotein in Pichia pastoris[J].J Viro Methods,2003,109(1):17-23.
169.White C E,Hunter M J,Meininger D P,White LR,Komives EA.Large-scale expression,purification and charaterization of small fragments of thrombomodulinahe roles of the sixth domain and of methionine[J].protein Eng,1995,8:1177-1187
170.Wilson C J,Zhan H,Swint Kruse,Matthews KS.The lactose repressor system:paradigms for regulation,allosteric behavior and protein folding[J].Cell Mol Life Sci.,2007,64(1):3-16.
171.World Animal Health,Reports on the animal health status and disease control methods and list A disease outbreaks,1996-1997.Paris,France:Statistics OIE.1997.
172.Xie J L,Zhang L,Ye Q,Xin L,Du P,Gan R.Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources[J].Biotechnol Lett,2003,25(2):173-177.
173.Xiong A S,Peng R H,Li X,Fan HQ,Yao QH,Guo M J,Zhang SL.Influence of signal peptide sequences on the expression of heterogeneous proteins in Pichia pastoris[J].Acta Bio-chimica &Biophysica Sinica,2003,35(2):154-160.
174.Yu Z L,Wu X J,Li DY,Yang S,Zhou Z,Cai J,Yuan ZY.Enhancement of the production of SAM by over expression of SAM synthetase in Pichia pastoris[J].Acta Biochimica & Biophysica Sinica,2003,35(2):127-132.
175.Yun C H.,Yim S K,Kim DH..Functional expression of human cytochrome P450 enzymes in Escherichia coli[J].Curt Drug Metab,2006,7(4):411-429.
176.Zhang Y,Guo YJ,Sun SH 1.Non-fusion expression in Escherichia coli,purification,and characterization of a novel Ca2+and phospholipids-binding protein annexin B 1[J].Protein Expr Purif.,2004,34(1):68-74.
177.Zinoview V.V,Tchikaev N.A,Chertov O.Y..Identification of the gene encoding vaccine virus immunodominant protein P35[J].Gene.1994,147:209-214.
2.程振涛,徐春志,岳筠,李永明,许乐仁,周碧君,文明.山羊痘病毒不同基因片段PCR诊断方法的研究[J].黑龙江畜牧兽医,2008,33(3):63-64.
3.程振涛,岳筠,李永明,许乐仁,王开功,周碧君,陈军义,李俊,江楠.山羊痘病毒TaqMan-MGB荧光定量PCR检测方法的建立与应用.生物工程学报,2009,25(3):464-472.
4.邓继军,周霞.山羊痘的诊治[J].吉林畜牧兽医,2004,(11):21.
5.丁打海.中国进出口动物检疫规范[M].中国农业出版社,1997,502-507.
6.丁选亚,乔传玲,陈艳,杨焕良,辛晓光,韩庆功,陈化兰.H3N2亚型猪流感病毒重组HA1蛋白间接ELISA诊断方法的建立[J].畜牧兽医学报,2008,39(9):1230-1234.
7.郭爱珍.山羊痘暴发流行的诊断[J].中国兽医杂志.2001:9.
8.郭建刚,郑敏,刘棋,李华明,邓朝阳,邹联斌,姚火春.山羊痘病毒P32基因在杆状病毒中的表达[J].中国兽医科技,2005,35(12):950-953.
9.郭巍,陈杰,黄保续,李晓成,吴发兴,张燕霞,李一经.应用PCR检测山羊痘病毒[J].中国兽医科技,2003,33(12):50-52.
10.郭巍,相文华,李一经.羊痘病毒P32蛋白的表达[J].中国兽医杂志,2005,41(3):8-10.
11.郭艳,付朝阳,宋素泉,高宏雷,王笑梅,王英,张厚双,王晓艳,胡建民.J亚群禽白血病ELISA抗体检测方法的建立.中国预防兽医学报,2005,27(4):304-307.
12.韩剑锋,宁宜宝,宋立.实时荧光PCR技术快速鉴别检测H5、H9、H7亚型禽流感灭活疫苗的研究[J]_生物工程学报,2007,23(5):953-957.
13.何光志,周碧君,虞天德,许乐仁.贵州山羊痘的病原学研究与血清学检测.山地农业生物学报,2004,23(4):300-304.
14.洪燕,谢忠文,施恒豫.人感染山羊痘五例[J].中华传染病杂志,2005,23(2):143.
15.金洪峰,韩梅.绵羊痘对养羊业的危害及防治基本对策[J].黑龙江畜牧兽医,2002,11:30-31.
16.康文玉,徐自忠,花群义,杨云庆,周晓黎,董俊,尹尚莲,高洪.羊痘病毒P32蛋白编码基因的克隆及表达[J].中国兽医科学,2006,36(6):454-459.
17.康文玉,徐自忠,高洪,花群义,周晓黎,杨云庆,董俊.羊痘病毒.中国畜牧兽医.2004,12:33-36.
18.李普森编著 食用动物疾病病理学[M]吉林科学技术出版社1989.5
19.李振刚主编,分子遗传学.北京,科学出版社,2000.
20.廖振续.山羊痘的暴发与防制[J].中国兽医杂志,1997,3:31-32.
21.林敏,倪凤娥.绵羊痘的诊治[J].河北畜牧兽医,2002,(10):33.
22.刘国英,乌日娜,李荣,等.牛睾丸原代细胞生产羊痘疫苗的试验[J].中国兽药杂志,2002,36(10):41.
23.刘棋,黄夏,郭建刚,陈义祥,郑敏,邓朝阳,李华明,邹联斌.山羊痘病毒的分离鉴定及生物学特性的研究[J].中国预防兽医学报,2006,(5):494-498.
24.龙沛然,周卫国,覃敏.山羊痘病毒的电子显微镜观察[J].中国兽医杂志,2004,40(8):23-24.
25.卢圣栋主编.现代分子生物学实验技术.北京,中国协和医科大学出版社,1999.
26.陆承平主编.兽医微生物学.北京,中国农业出版社,2001.
27.罗辽复,李晓琴.tRNA丰度是影响蛋白质二级结构形成的一个因素[J].内蒙古大学学报,2003,34(5):519-529.
28.马世东.非洲猪瘟的间接ELISA诊断试剂盒的研究[D].新疆农业大学硕士论文.
29.娜仁.绵羊痘的防治[J].中国动物保健,2002,5:49.
30.彭毅,步威,康良仪.甲醇酵母表达系统.生物技术通报,2000.(1):38-41.
31.瞿礼嘉等著.现代生物技术导论(第一版).高等教育出版社,北京,1998.
32.任增亮,堵国成,陈坚.大肠杆菌高效表达重组蛋白策略[J].中国生物工程杂志,2007,27(9):103-109.
33.萨姆布鲁克J,拉赛尔D W.分子克隆实验指南.[M].科学出版社.2002.
34.时成波,吕安国,吴文芳,杨立泉,冯家勋,柏学亮.改造稀有密码子提高SEA蛋白表达量[J].生物工程学报,2002,18(4):477-480.
35.世界动物卫生组织.哺乳动物、禽、蜜蜂A和B类疾病诊断试验和疫苗标准手册[M].农业部畜牧兽医局.中国农业科学技术出版社,2002:156-166.
36.孙桂玲,李志刚,宋明,林森.羊痘病的防治[J].吉林畜牧兽医,2004,6:42-43.
37.索效军,张年,李晓峰,付忠燕,陈明新.羊痘的诊断与防治[J].湖北畜牧兽医,2004,6:29-30.
38.王殿军.绵羊痘的防治[J].中兽医医药杂志.1995,1:47.
39.王开功,虞天德,何光志,周碧君,许乐仁,孙道忠.应用反向间接血凝试验检测山羊痘病毒抗原.山地农业生物学报,2005,24(1):29-32.
40.王开功,虞天德,张大权,田志敬,阴正兴,许乐仁,周碧君.贵州省首次暴发山羊痘的诊断研究.动物医学进展,2003,24(4):116-118.
41.王效田,兰朋云,夏明龙,等.山羊痘的诊断与防制[J].中国兽医杂志,2001,37(3):28-29.
42.文明,程振涛,岳筠,李永明,周碧君.山羊痘病毒P32基因序列分析及其B细胞表位预测.生 物技术,2007,17(5):12-14.
44.吴乃虎主编.基因工程原理(第2版,上册).北京,科学出版社,2001
45.许乐仁,周碧君,阴正兴.山羊痘病变的超微病理学研究[J].畜牧兽医学报,2005,36(5):492-497.
46.许乐仁,周碧君.山羊痘的研究概况[J].内蒙古畜牧科学,2003,4:13-15.
47.阴正兴,许乐仁,周碧君,虞天德,乐正中.山羊痘的病理形态学观察[J].中国预防兽医学报,2005,27(5):402-407.
48.殷震,刘景华主编.动物病毒学(第二版)[M].北京:科学出版社,1997,960-963.
49.俞乃胜.山羊疾病学[M].云南科技出版社,2000.
50.张端阳,周永安,王俊民.羊痘一家4例报告[J].中国皮肤性病学杂志,2003,3:196-197.
51.张前群,马承俊.山羊痘暴发流行的防制与思考[J].畜禽业,2004,9:20.
52.张小青,丁明孝.痘苗病毒诱导HeLa细胞的凋亡[J].中国病毒学,1998,13(4):364-368.
53.张振岚,夏明龙.南京部分地区暴发流行山羊痘的诊断与防制[J]畜牧与兽医2000,32(4)23-24.
54.周碧君,虞天德,阴正兴,许乐仁.山羊痘病毒粒子的电子显微镜观察[J].中国预防兽医学报,28(3):316-319.
55.周锦萍.山羊痘病毒的分离与鉴定[J].上海畜牧兽医通讯,2002,(3):44.
56.周则迅,袁汉英,何炜.乙肝病毒表面抗原SA-28融合基因在酵母中的组成型表达[J].复旦学报(自然科学版),2000,39(3):264-268.
57.Adlakha S C,Bansal M P and Malik B S.Studies on sheep and goat-poxviruses I.Adaptation of the viruses in laboratory animals and tissue culture[J].Indian J Anim Sci,1971,41:171-175.
58.Allerson CR,Martinez A,Yikilmaz E,Rouault TA.A high-capacity RNA affinity column for the purification of human IRP1 and IRP2 overexpressed in Pichia pastoris[J].Rna-A Publication of the Rna Society,2003,9(3):364-374.
59.Annadana S,Schipper B,Beekwilder J,Outchkourov N,Udayakumar M,Jongsma MA.Cloning.functional expression in Pichia pastoris,and purification of potato cystatin and multicystatin[J].Journal of Bioscience & Bioengineering,2003,95(2):118-123.
60.Arnau J,Lauritzen C,Petersen GE,Pedersen J.Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins[J].Protein Expr Purif,,2006,48(1):1-13.
61.Baixeras E.,Cebrian,A.Vaccinia virus-induced apoptosis in immature B lymphocytes:role of cellular Bcl-2[J].Virus Research,1998,58:107-113
62. Bei JL, Wang JW, Wang XZ, Long QX, Yang L, Deng YY. High expression of LIP1 in Pichia pastoris[J]. Acta Biochimica & Biophysica Sinica, 2003, 35 (4): 366-370.
63. Bowers LM, Lapoint K, Ant hony L, Pluciennik A, Filutowicz M.Bacterial expression system with tightly regulated gene expression and plasmid copy number [J] .Gene, 2004, 340 (1):11-18.
64. Cam V M, Kitching R P, Hammond J M., Chand P. Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus[J]. J. Virol. Methods, 1994,49:285-294.
65. Cam V M. An antigen trapping ELISA for the detection of capripoxvirus in tissue culture supernatant and biopsy samples[J]. J. Virol. Methods, 1995, 51: 95-102.
66. Cam V M. Control of capripoxvirus infections[J]. Vaccine, 1993,11:1275-1279.
67. Cam V. M., Kitching R. P., Hammond J. M., et al. Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus[J]. J. Virol. Methods, 1994,49:285-294.
68. Chadd H E , Chamow S M. Therapeutic antibody expression technology [J]. Curr Opin Biotechnol, 2001,12: 188-194.
69. Chand P, Kitching R P & Black D N. Western blot analysis of virus-specific antibody responses to capripoxvirus and contagious pustular dermatitis infections in sheep[J]. Epidemiol. Infect,1994,113:377-385.
70. Chen H , Xu Z, Cen P. High-level expression of human beta-defensin-2 gene with rare codons in E.coli cell-free system [J].Protein Pept Lett, 2006, 13 (2) : 155-162.
71. Chou P. Y, Fasman G.D. Prediction of the secondary structure of protein conformation[M].New York: Plenum Press, 1990, 549-589.
72. Chumpolkulwong N , Aakamoto K, Hayashi A , Iraha F, Shinya N, Matsuda N, Kiga D,Urushibata A, Shirouzu M, Oki K, Kigawa T, Yokoyama S. Translation of 'rare' codons in a cell-free protein synthesis system from Escherichia coli [J]. J Struct Funct Genomics, 2006, 7(1): 31-36.
73. Cran V M. Control of capripoxvirus infection[J].Faccine,1993,11(3):1275-1279.
74. Cregg J M, Madden K R, Barringer K J, Thill GP, Stillman CA. Functional characterization of the two alcohol oxidase genes from the yeast pichia pastoris[J]. Mol Cell Biol, 1989, 9:1316-1323.
75. Cregg J M, Vedvick T S, Raschke W C. Resent advances in the expression of foreign genes in pichia pastoris[J]. Bio/Technology, 1993, 11:905-910.
76. David R, Higgins, James M. Pichia Protocols. New jersey, Humana Press Inc, 1998:193-200
77. Davies F G, Krauss H, Lund L J, Taylor M. The laboratory of lumpy skin disease[J]. Res Vet Sci, 1971,12:123-127.
78.Davies F G.& Atema C.The antibody response in sheep infected with a Kenyan sheep and goat pox virus[J].J Comp.Pathol.,1978,88:205-210.
79.Davies F G.& Mbugwa G.The alterations in pathogenicity and immunogenicity of a Kenya sheep and goat pox virus on serial passage in bovine fontal muscle cell cultures[J].J.Comp.Pathol.,1985,95:565-576.
80.Davies FG.Characteristics of a virus causing a pox disease in sheep and goats in Kenya with observations on the epidemilogy and control[J].Journal of Hygiene,Cambridge,1976,76:163-171.
81.Dong X,Tang B,Li J,Xu Q,Fang S,Hua Z.Expression and Purification of Intact and Functional Soybean(Glycine max) Seed Ferritin Complex in Escherichia coli[J].J Microbiol Biotechnol,2008,18(2):299-307.
82.Ellis S B,Brust P F,Koutz P J,Waters AF,Harpold MM,Gingeras TR.Isolation of alcohol oxidase and two other methanol requlatable genes from the yeast pichia pastoris[J].Mol Cell Biol,1985,5:1111-1121.
83.Emini E.A,Hughes J.V,Perlow D.S,Boger J.Induction of hepatitis a virus-neutralizing antibody by a virus-specific synthetic peptide[J].J Virol.,1985,55:836-839.
84.Esposito J.P and F.Fenner.Poxviruses.2001.2885-2921
85.Fidler A E,Lin J S,Chie W N,Ng Chie W,Western A,Stent V,McNatty KP.Production of biologically active tethered ovine FSH beta alpha by the methylotrophic yeast Pichia pastoris[J].J Mol Endocrinol,2003,30(2):213-225.
86.Garner M G,Sawarkar S D,Brett E K,Edwards JR,Kulkarni VB,Boyle DB,Singh SN.The extent and impact of sheep pox and goat pox in the state of Maharashtra,India[J].Trop Anim Health Prod,2000,32(4):205-23.
87.Garnier J,Osguthorpe D.J and Robson B.Analysis of the accuracy and implications of simple method for prediction the secondary structure of globular proteins[J].J..Mol.Biol.,1978,12:97-120.
88.Gen Wang,John W.Myxoma Virus M11L Prevents Apoptosis through Constitutive Interaction with Bake[J].Journal of Virology,2004,(7):7097-7111
89.Georgiou G,Segatori L.Preparative expression of secreted proteins in bacteria:status report and future prospects[J].Curr Opin Biotechnol,2005,16(5):538-545.
90.Gershon P.D.,Ansell D.M.and Black D.N.A comparison of the genome organization of capripoxvirus with that of the orthopoxviruses[J].J Virol.,1989,63(11):4703-4708.
91. Gershon P.D., and Black D.N. The nucleotide sequence around the capripoxvirus thymidine kinase gene reveals a gene shared specifically with leporipoxvirus[J]. J Gen Virol., 1989,70(3):525-533.
92. Gershon, P.D., Black.A. Capripoxvirus pseudogene whose only intact homologys are in other poxvirus genomes[J]. Virology, 1989, 172:350-354.
93. Hanquier J, Sorlet Y, Desplancq D, Baroche L, Ebtinger M, Lefevre JF, Pattus F, Hershberger CL, Vertes AA. A single mutation in the activation site of bovine trypsinogen enhances its accumulation in the fermentation broth of the yeast Pichia pastoris[J]. Applied & Environmental Microbiology, 2003,69 (2): 1108-1113.
94. Hay S., Kannourakis G. A time to kill: viral manipulation of the cell death program[J]. J Gen Virol, 2002, 83: 1547-1564.
95. Heine H. G., Stevens M. P., Foord AJ. A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog cf the vaccine virus H3L gene[J]. Journal of Immunological Methods, 1999, 227:187-196.
96. Heine H.G, Lin C.L., Chung C.S. Vaccine virus envelope H3L protein binds to cell surface heparan sulfate and is important for intracellular mature virion morphogenesis and virus infection in vivo[J].Virol.2000,74(4):3353-3365.
97. Hirokawa T, Boon-Chieng S. and Mitaku S. SOSUI: Classification and secondary structure prediction system for membrane proteins [J]. Bioinformatics, 1998,14(4): 378-379.
98. Hoffman SK, Susani M, Ferreira F, Jertschin P, Ahorn H, Steiner R, Kraft D, Scheiner O,Breiteneder H. High-level expression and purification of the major birch pollen allerge, BetV [J].Protein Expr Purif., 1997, 9 (1): 33-39.
99. Hopp T.P, Woods K. R. Prediction of protein antigenic determinants from amino acid sequence[J].Proc. Natl. Acad Sci USA,1981,78(6): 3824-3828.
100. Hosamani M, Nandi S, Mondal B, Singh RK, Rasool TJ, Bandyopadhyay SK. A Vero cell-attenuated Goatpox virus provides protection against virulent virus challenge[J]. Acta Virol.,2004,1(48):15-21.
101. Hosamni M, Mondal B, Tembhurne P A, Bandyopadhyay SK, Singh RK, Rasool TJ.Differentiation of Sheep Pox and Goat Poxviruses by Sequence Analysis and PCR-RFLP of P32 Gene[J].Virus Genes, 2004,29(1):73-80.
102. Inouye M. The discovery of mRNA interferases: implication in bacterial physiology and application to biotechnology [J] J Cell Physi-ol., 2006, 209 (3): 670-676.
103. Ireland D.C, Binepal Y.S. Improved detection of capripoxvirus in biopsy samples by PCR[J].Journal of Virological Methods, 1998, 74:1-7.
104.Ivanov A V,Korovina A N,Tunit skaya,Kostyuk DA,Rechinsky VO,Kukhanova MK,Kochetkov SN.Development of the system ensuring a high-level expression of hepatitis C virus non-structural NS5B and NS5A proteins[J].Protein Expr Purif,2006,48(1):14-23.
105.Jahic M,Gustavsson M,Jansen A K,Martinelle M,Enfors SO.Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes[J].J Biotechnol,2003,102(1):45-53.
106.Jameson B.A,Wolf H.The antigenic index:A novel algorithm for prediction antigenic determinants[J].Comput.Appl.Biosci.,1988,4:181-186.
107.Jin Su,Gen Wang.Myxoma Virus M11L Blocks Apoptosis through Inhibition of Conformational Activation of Bax at the Mitochondria[J].Journal of Virology,2006,80(3):1140-1151
108.Jinbiao L,Dong W,Guozhi W,Wang G.High-level expression and single-step purification of recombinant Bacillus anthracis protective antigen from Escherichia coli[J].Biotechnol Appl Biochem,2008,4(7):102-110.
109.Johnson K L.,Vaillant F.,Lawen A.Protein tyrosine kinase inhibitors prevent didemnin B-induced apoptosis in HL-60 cells[J].FEBS Lett.,1996,383:1-5.
110.Jonasson P.,Liljeqvist S.,Nygren P A.,Stahl S.Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli[J].Biotechnology and Applied Biochemistry,2002,35(2):91-105.
111.Joseph M.,Fernandez,James P.Gene Expression Systems[M].New-york,Academic Press,1999.
112.Joshi R K,Chandra R,Rao V D P.Cytopathogenicity of goat poxvirus[J].Indian Journal of Comparative Microbiology Immunology and Infectious Diseases,1994,15:35-36.
113.Joshi R K.Growth behavior of sambalpur strain of goat poxvirus on chorioallantoic membrane of developing chicken embryo[J].Indian Journal of Virology,1995,12:109-111.
114.Julius D,Brake A,Blair L,Kunisawa R,Thorner J.Isolation of putative structural gene for the lysine-arginine-cleaving endopeptidese required for processing of yeast prepro-a-factor[J].Cell,1985,37:1075.
115.Kang Y,Son M S.,Hoang TT.One step engineering of T7-expression strains for protein production:increasing the host-range of the T7-expression system[J].Protein Expr Purif.,2007,55(2):325-333.
116.Karplus P.A,Schultz G.Prediction of chain flexibility in proteins[M].Naturwissenschaften,1985,72:212-213.
117.Kawai R,Yoshida M,Tani T,Igarashi K,Ohira T,Nagasawa H,Samejima M.Production and characterization of recombinant Phanerochaete chrysosporium beta-glucosadase in the methylotrophic yaest Pichia pastoris[J].Bioscience Biotechnology & Biochemistry,2003,67(1): 1-71
118.Kitching R P.Progress towards sheep and goat pox vaccines[J].Vaccine,1983,10(1):4-9.
119.Kitching R P,Hammond J M,Black D N.Studies on the major common precipitating antigen of capripoxvirus[J].J Gen Virol,1986,67(Pt1):139-148.
120.Kitching R P,Taylor W P.Clinical and antigenic relationship between isolates of sheep and goat pox viruses[J].Tropical Animal Health and Production,1985,17(2):64-74121.Kitching R P.Sheep and goat poxviruses[J].In:Webster RG,Granoff A,Encylopedia of Virology.London:Academic Press,1994,1160-1165
122.Koutz P,Davis G R,Stillman C,Barringer K,Cregg J,Thill G.Structural comparison of the Pichia Pastoris alcohol oxidase genes[J].Yeast,1989,5:167-177.
123.Kuba H,Furukawa A,Okajima T,Furukawa K.Efficient bacterial production of functional antibody fragments using a phagemid vector[J].Protein Expr Purif,2008,58(2):292-300.
124.Kyte J,Doolittle R.F.A simple method for displaying the hydropathic character of a protein[J].J Mol.Biol.,1982,157:105-132.
125.Li Y H.,Bai J J.,Jian Q..Expression of common carp growth hormone in the yeast Pichia pastoris and growth stimulation of juvenile tilapia(Oreochromis niloticus)[J].Aquaculture,2003,216(1-4):329-341.
126.Liang R.,Liu J.,Ren Q.,Ren Q,Liang P,Lin Z,Xie X.A T7-expression system under temperature control could create temperature-sensitive phenotype of target gene in Escherichia coli[J].J Microbiol Methods,2007,68(3):497-506.
127.Mac W T.High-Yield Secretion of recombinant geltions by Pichia Pastris[J].Yeast,1999,15:1087-1096.
128.Madhusudan hosamani,Bimalendu mondal,Prabhakar a.tembhume.Differentiation of Sheep Pox and Goat Poxviruses by Sequence Analysis and PCR-RFLP of P32 Gene[J].Virus Genes,2004,29(1):73-80.
129.Manganal-Vougiouka O.,Markoulatos P.,Koptopoulos G.,Nomikou K,Bakandritsos N,Papadopoulos P.Sheep poxvirus identification from clinical specimens by PCR,cell culture,Immunofluorescence and agar gel immunoprecipitation assay[J].Molecular and Cellular Probes,2000,14:305-310.
130.Mangana-Vougiouka O.,Markoulatos P.,Koptopoulos G.,Koptopoulos G,Nomikou K,Bakandritsos N,Papadopoulos O..Sheep poxvirus identification by PCR in cell cultures[J].J Virol Methods,1999,77(1):75-79.
131.Markoulatos P.,Manganal-Vougiouka O.Detection of sheep poxviruse skin biopsy samples by a multiplex polymerase chain reaction[J].Journal of Virological Methods,2000,84(2):161-167.
132.Marc W T.High-yield secretion of recombinant gelation by pichia pastoris[J].yeast,1999,15:108-109.
133.Matthews R E F.Classification and nomenclature of viruses[M].Intervirology,1982,17:1-199.
134.Michael J O.Donbue.Over expression in Pichia Pastries and crystallization of an elicidor protein secreted by the phytopathogenic fungus[J].Protein Experessionand Purification,1996,8:254-261.
135.Nagpal A K,Vihan V S,Singh D.,Edwards JR,Kulkarni VB,Boyle DB,Singh SN.Epidemiological study of an outbreak of goat-pox and its effect on the performance of kids[J].Indian Journal of Animal Sciences,1990,60(5):544-546
136.Nomura S I,Inamori K I,Muta T,Yamazaki S,Sunakawa Y,Iwanaga S,Takeshige K.Purification and characterization of human soluble CD14 expressed in Pichia pastoris[J].Protein Expression & Purification,2003,28(2):310-320.
137.Nourizad N.,Ehn M.,Gharizadeh B.,Hober S,Nyren P.Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase(apyrase) from potato tubers(Solanum tuberosum)[J].Protein Expression & Purification,2003,27(2):229-237.
138.Nuc P.,Nuc K.Recombinant protein production in Escherichia coli[J].Postepy Biochem,2006,52(4):448-456.
139.Ou J,Yamada Y,Nagahisa K,Hirasawa T,Furusawa C,Yomo T,Shimizu H.Dynamic change in promoter activation during lysine biosynthesis in Escherichia coli cells[J].Mol Biosyst,2008,4(2):128-134.
140.Plowright W.,Ferris P.D.The growth and cytopathogenicity of sheep poxvirus in tissue culture[J].Br.J..Exp.Pathol.,1958,39:424-435.
141.Quyen D T.,Schmidt-Dannert C.,Schmid RD.High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichia pastoris and some properties of the recombinant lipase[J].Protein Expression & Purification,2003,28(1):102-110.
142.Rafyi A,Ramyar H.Goat pox in Iran,serial passage in goats and the developing eggs and relationship with sheep pox[J].J Comp Pathol,1959,69(2):141-147.
143.Ran T V,Bandyopadhyay S K.A comprehensive review of goat pox and sheep pox and their diagnosis[J].Anim Health Res Rev,2000,1(2):127-136.
144.Rao T V,Malik P,Nandi S,Negi BS.Evaluation of immunocapture ELISA for diagnosis of goat pox[J].Acta Virol,1997,41(6):345-348.
145.Rao T V,Negi B S.Evaluation of different serological tests for the diagnosis of goat pox using soluble antigen[J].Trop Anim Health Prod.,1997,29(4):235-239.
146.Rao T.V.S.and Bandyopadhyay S.K.A comprehensive review of goat pox and sheep pox and their diagnosis[J].Animal Health Research Reviews,2000,1(2);127-136
147.Romanos M A,Scorer C A,Clare J J.Foreign gene expression in yeast:a review[J].Yeast,1992,8(6):423-488.
148.Romanos M A.Advances in the use of Pichia pastoris for high-level gene expression[J].Current Opinion in Biotechnology,1995,6:527-533.
149.Roulston A,Marcellus R C,Branton P E.Viruses and apoptosis[J].Annual Rev Microbiol,1999,53:577-628.
150.Sato T,Terabe M,Watanabe H.Codon and base biases after the initiation codon of the open reading frames in the Escherichia coli genome and their influence on the translation efficiency[J].J Biochem(Tokyo),2001,129(6):851-860.
151.Scorer C A,Clare J J,Mccombie W R.,Romanos MA,Sreekrishna K.Rapid selection using G418 of high copy number tramsformants of pichia pastoris for high-level foreign gene expression[J].Bio/Technology,1994,12:181-174.
152.Sedhukhan T,Nag NCl.Application of dot-ELISA in the diagnosis of goat pox outbreak[J].Indian Veterinary Journal,1998,75:841-842.
153.Shchelkunov S N.The orthopoxviral genome[M].Mol Biol.,1996,30:1-16.
154.Shi X Z,Karkut T,Chamankhah M,Alting-Mees M,Hemmingsen SM,Hegedus D.Optimal conditions for the expression of a single-chain antibody(scFv) gene in Pichia pasitoris[J].Protein Expression & Purification,2003,28(2) 321-330.
155.Siegei R S.Methylotroyphic yeast Pichia pastoris produced in high-cell-density fementation with high cell yields as vehide for recombinant protein production[J].Biotechnology and Bioegineering,1989,34:403-404.
156.Siegel R S,Buckholz R G,Thill GP.Production of epidermal growth factor in methylogrophic yeast cells[M].European Patent Application,1990.
157.Singh B,Rai A.Virion polypeptides of sheep poxvirus[J].Indian Vet Med J,1991,15(4):239-244.
158.Tantawi H.H,Shony M.O,Hassan F.K.Isolation and identification of the Sersenk strain of goat pox virus in Iraq[J].Trop Anim Health Prod,1979,11(4):208-210.
159.Terpe K.Overview of bacterial expression systems for heterologous protein production:from molecular and biochemical fundamentals to commercial systems[J].Appl Microbiol Biotechnol,2006,72(2):211-222.
160.Trinh L B.,Phue J N.,Shiloach J.Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris[J].Biotechnology & Bioengineering,2003,82 (4):438-444.
161.Tschopp J F,Sverlow G,Kosson R.High-level secretion of glycosylated invertase in the methylotrophic yeast,Pichia pastoris[J].Bio Technology,1987,5:1305-1308.
162.Tulman E R,Afonso C.L,Lu Z,Zsak L,Sur JH,Sandybaev NT,Kerembekova UZ,Zaitsev VL,Kutish GF,Rock DL.The genomes of sheeppox and goatpox viruses[J].J.Virol.,2002,76(12):6054-6061.
163.Tulman E R,Afonso C L.,Kutish G F,Zsak L,Sur JH,Sandybaev NT,Kerembekova UZ,Zaitsev VL,Kutish GF,Rock DL.Genome of lumpy skin disease virus[J].J.Virol,2001,75:7122-7130.
164.Ved Prakash,Chandra R,Rao V D P.Cultivation of goat pox virus in establish cell line[J].Indian J Virol,1994,10:60-63.
165.Wang W,Sun Y H,Wang Y P,Zhu ZY.Expression of grass carp growth hormone in the yeast Pichia pastoris[J].Acta Genetica Sinica,2003,30(4):301-306.
166.Waterham H R,Digan M E,Koutz P J,Lair SV,Cregg JM.Isolation of the Pichia pastoris glyceraldehyde-3-phosphate deydyogenase gene and regulation and use of its promoter[J].Gene,1997,186:37-44.
167.Wegner G.Emerging applications of methytrophic yeasts FEMS[J].Microbiol Rev,1990,87:279-284.
168.Wei HY,Jiang L F,Xue YH,Fang DY,Guo HY.Secreted expression of dengue virus type2full-length envelope glycoprotein in Pichia pastoris[J].J Viro Methods,2003,109(1):17-23.
169.White C E,Hunter M J,Meininger D P,White LR,Komives EA.Large-scale expression,purification and charaterization of small fragments of thrombomodulinahe roles of the sixth domain and of methionine[J].protein Eng,1995,8:1177-1187
170.Wilson C J,Zhan H,Swint Kruse,Matthews KS.The lactose repressor system:paradigms for regulation,allosteric behavior and protein folding[J].Cell Mol Life Sci.,2007,64(1):3-16.
171.World Animal Health,Reports on the animal health status and disease control methods and list A disease outbreaks,1996-1997.Paris,France:Statistics OIE.1997.
172.Xie J L,Zhang L,Ye Q,Xin L,Du P,Gan R.Angiostatin production in cultivation of recombinant Pichia pastoris fed with mixed carbon sources[J].Biotechnol Lett,2003,25(2):173-177.
173.Xiong A S,Peng R H,Li X,Fan HQ,Yao QH,Guo M J,Zhang SL.Influence of signal peptide sequences on the expression of heterogeneous proteins in Pichia pastoris[J].Acta Bio-chimica &Biophysica Sinica,2003,35(2):154-160.
174.Yu Z L,Wu X J,Li DY,Yang S,Zhou Z,Cai J,Yuan ZY.Enhancement of the production of SAM by over expression of SAM synthetase in Pichia pastoris[J].Acta Biochimica & Biophysica Sinica,2003,35(2):127-132.
175.Yun C H.,Yim S K,Kim DH..Functional expression of human cytochrome P450 enzymes in Escherichia coli[J].Curt Drug Metab,2006,7(4):411-429.
176.Zhang Y,Guo YJ,Sun SH 1.Non-fusion expression in Escherichia coli,purification,and characterization of a novel Ca2+and phospholipids-binding protein annexin B 1[J].Protein Expr Purif.,2004,34(1):68-74.
177.Zinoview V.V,Tchikaev N.A,Chertov O.Y..Identification of the gene encoding vaccine virus immunodominant protein P35[J].Gene.1994,147:209-214.